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CN110047562B - Information structure and method for titer detection based on enzyme activity determination method - Google Patents

Information structure and method for titer detection based on enzyme activity determination method Download PDF

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CN110047562B
CN110047562B CN201910399574.3A CN201910399574A CN110047562B CN 110047562 B CN110047562 B CN 110047562B CN 201910399574 A CN201910399574 A CN 201910399574A CN 110047562 B CN110047562 B CN 110047562B
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data
original
titer
module
detection
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CN110047562A (en
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郝士婧
朱敏莹
常超
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Shanghai Shangyao First Biochemical Pharmaceutical Co ltd
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Shanghai Shangyao First Biochemical Pharmaceutical Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16CCOMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/90Programming languages; Computing architectures; Database systems; Data warehousing

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses an information structure and a method for titer detection based on an enzyme activity determination method, which comprises an ultraviolet-visible spectrophotometer dynamics detection system and a Laboratory Information Management System (LIMS), wherein the ultraviolet-visible spectrophotometer dynamics detection system provides raw data acquisition and raw data storage records and provides a raw data report, and the Laboratory Information Management System (LIMS) captures a test data storage raw map provided by the raw data report and obtains a detection result. The information structure and the method for titer detection based on the enzyme activity determination method have the advantages of simple structure and high intelligent degree, adopt more scientific means to analyze and process data, improve the detection efficiency, reduce the deviation probability and ensure the integrity of the detection data.

Description

Information structure and method for titer detection based on enzyme activity determination method
Technical Field
The invention relates to the technical field of enzyme drug potency measurement, in particular to an information structure and a method for potency detection based on an enzyme activity measurement method.
Background
The laboratory informatization management system brings new possibility for medicine detection, and can provide a more efficient experiment mode and more accurate data processing conditions while effectively ensuring the integrity of laboratory data. The enzyme activity detection integrates the influences of constant temperature control, enzyme and substrate reaction, linear absorbance change, data straight line fitting and the like, the test consumes more time, and the occurrence probability of deviation is higher. At present, a stopwatch is used for timing in the test, data of corresponding time points are manually recorded, and a titer result is obtained through simple analysis after original data are obtained. This method is highly demanding for the inspector, and requires years of experience from related experiments and skilled operators to obtain reliable data. Laboratories hope to adopt an informatization means, more systematically eliminate the uncertainty in the inspection, reduce the difficulty of experimental analysis, and obtain more reliable and accurate data.
According to the specification of the Chinese pharmacopoeia 2015 year edition, under the condition of excessive substrate and specified buffer solution and solution, the ultraviolet-visible spectrophotometry is adopted, and the measurement is carried out at a specific wavelength under the condition of a certain temperature. The absorbance is read every 30 seconds, the change rate of the absorbance is constant, and the constant time is not less than 3 minutes. The change rate of absorbance every 30 seconds is controlled in a certain area, the absorbance is used as a vertical coordinate, the time is used as a horizontal coordinate to plot, the absorbance of a straight line part formed in 3 minutes is taken, and finally, the efficacy value is calculated.
The informatization method adopts an automatic reading method of an ultraviolet-visible spectrophotometer, and because the automatic reading of the instrument can not identify interference data in time, the absorbance value read in the enzyme reaction process has certain fluctuation probability, and the fluctuation data can bring immeasurable influence to the detection. The establishment of methods for effectively identifying and eliminating interference data in the testing process and statistical methods for obtaining titer results is imperative.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an information structure and a method for titer detection based on an enzyme activity determination method, which solve the problems in the background technology.
In order to achieve the purpose, the invention is realized by the following technical scheme:
an information structure and method for titer detection based on enzyme activity determination method comprises an ultraviolet-visible spectrophotometer dynamics detection system and a Laboratory Information Management System (LIMS), wherein the ultraviolet-visible spectrophotometer dynamics detection system provides raw data acquisition and raw data storage records and provides a raw data report, the Laboratory Information Management System (LIMS) grabs a test data storage raw map provided by the raw data report and obtains a detection result, the ultraviolet-visible spectrophotometer dynamics detection system comprises an ultraviolet-visible spectrophotometer and ultraviolet-visible spectrophotometer control application software, the Laboratory Information Management System (LIMS) comprises a PDF file analysis module and a laboratory information management system electronic raw record (ELN) module, and the laboratory information management system electronic raw record (ELN) module comprises a test record module, The system comprises a raw data recording module, a data analysis module, a change rate straight line fitting graph module and an accessory module.
Optionally, the method comprises the following steps:
A. the ultraviolet-visible spectrophotometer is used for controlling application software to set the automatic reading interval of the dynamic detection parameters, the reading time interval is shortened, and the data acquisition quantity is increased;
B. filling test records in the electronic original records;
C. the acquired original data is given by a report file provided by ultraviolet visible spectrophotometer control application software, a PDF file analysis module is called in a laboratory information management system, and the original data and map records are stored in the system;
D. the original data is recorded and displayed by an original data recording module in the electronic original record, and an original map is stored in an attachment module in the electronic original record in an attachment form;
E. the data analysis module in the electronic original record groups the original data, and respectively judges the conformity so as to eliminate interference data;
F. and performing linear fitting analysis and titer calculation on the effective data group to obtain reference data such as linear fitting correlation coefficient, titer mean value, titer median and the like, and obtaining a titer determination result to form a change rate linear fitting graph.
Optionally, in step a, the kinetic parameters set by the uv-visible spectrophotometer control application software specifically include: the automatic reading time interval is 5 seconds, total 330 seconds, and the data acquisition amount is 67.
Optionally, in the step C, a parsing script of a PDF file is written according to a PDF report file format by using a parsing script module in labware7.0, and the original data is captured into the system.
Optionally, in the step D, an electronic original record is created, the original data is recorded in the original data recording module, and the original map attachment is uploaded to the corresponding electronic original record.
Optionally, in the step E, data points that are one every 30 seconds are grouped into data groups, and a formula is written using an electronic original recording standard function to determine whether the data level difference meets the specification, and if the level difference does not meet the specification, the data groups are removed, and the data groups are not subjected to straight line fitting analysis.
Optionally, in the step F, the correlation coefficient, the titer mean and the titer median of each group of data are obtained according to a statistical method, and the titer result, the used original data and the important reference data are all displayed in the test recording module.
The invention provides an information structure and a method for titer detection based on an enzyme activity determination method, which have the following beneficial effects: the information structure and the method for titer detection based on the enzyme activity determination method have the advantages of simple structure and high intelligent degree, adopt more scientific means to analyze and process data, improve the detection efficiency, reduce the deviation probability and ensure the integrity of the detection data.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
An information structure and method for titer detection based on enzyme activity determination method comprises an ultraviolet-visible spectrophotometer dynamics detection system and a Laboratory Information Management System (LIMS), wherein the ultraviolet-visible spectrophotometer dynamics detection system provides raw data acquisition and raw data storage records and provides a raw data report, the Laboratory Information Management System (LIMS) grabs a test data storage raw map provided by the raw data report and obtains a detection result, the ultraviolet-visible spectrophotometer dynamics detection system comprises an ultraviolet-visible spectrophotometer and ultraviolet-visible spectrophotometer control application software, the Laboratory Information Management System (LIMS) comprises a PDF file analysis module and a laboratory information management system electronic raw record (ELN) module, and the laboratory information management system electronic raw record (ELN) module comprises a test record module, The system comprises a raw data recording module, a data analysis module, a change rate straight line fitting graph module and an accessory module, and further comprises the following steps:
A. the method comprises the following steps of setting an automatic reading interval of dynamic detection parameters by using ultraviolet-visible spectrophotometer control application software, shortening the reading time interval, and increasing the data acquisition amount, wherein the dynamic parameters set by the ultraviolet-visible spectrophotometer control application software are specifically as follows: the automatic reading time interval is 5 seconds, totally 330 seconds, and the data acquisition amount is 67;
B. filling test records in the electronic original records;
C. the collected original data is given by a report file provided by ultraviolet visible spectrophotometer control application software, a PDF file analysis module is called in a laboratory information management system, the original data and map records are stored in the system, specifically, in the step, a parsing script of the PDF file is compiled by using a parsing script module in LabWare7.0 according to a PDF report file format, and the original data is captured into the system;
D. the method comprises the following steps that original data are recorded and displayed by an original data recording module in an electronic original record, an original map is stored in an attachment module in the electronic original record in an attachment form, specifically, in the step, the electronic original record is created, the original data are recorded in the original data recording module, and meanwhile, the attachment of the original map is uploaded to the corresponding electronic original record;
E. the data analysis module in the electronic original record groups the original data, and respectively performs conformance judgment to remove interference data, specifically, in the step, data points of which one data point is formed every 30 seconds are grouped into data groups, a formula is compiled by using the standard function of the electronic original record to judge whether the data level difference meets the regulation, if the level difference does not meet the regulation, the data group is removed, and the data group is not subjected to linear fitting analysis;
F. and performing linear fitting analysis and valence calculation on the effective data group to obtain reference data such as linear fitting correlation coefficient, valence mean value, valence median and the like, and obtaining a valence measurement result to form a change rate linear fitting graph.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. A method of constructing an information structure for titer detection based on enzyme activity assay, the structure comprising an ultraviolet-visible spectrophotometer dynamics detection system and a Laboratory Information Management System (LIMS), characterized in that: the dynamics detection system of the ultraviolet-visible spectrophotometer provides original data acquisition and original data storage records and provides an original data report, the Laboratory Information Management System (LIMS) captures a test data storage original map provided by the original data report and obtains a detection result, the dynamics detection system of the ultraviolet-visible spectrophotometer comprises the ultraviolet-visible spectrophotometer and ultraviolet-visible spectrophotometer control application software, the Laboratory Information Management System (LIMS) comprises a PDF file analysis module and a laboratory information management system electronic original record (ELN) module, and the laboratory information management system electronic original record (ELN) module comprises a test record module, an original data record module, a data analysis module, a change rate straight line fitting map module and an accessory module; wherein, the method comprises the following steps:
A. the ultraviolet-visible spectrophotometer is used for controlling application software to set the automatic reading interval of the dynamic detection parameters, the reading time interval is shortened, and the data acquisition quantity is increased;
B. filling test records in the electronic original records;
C. the acquired original data is given by a report file provided by ultraviolet visible spectrophotometer control application software, a PDF file analysis module is called in a laboratory information management system, and the original data and map records are stored in the system;
D. the original data is recorded and displayed by an original data recording module in the electronic original record, and an original map is stored in an attachment module in the electronic original record in an attachment form;
E. the data analysis module in the electronic original record groups the original data, and respectively judges the conformity so as to eliminate interference data;
F. and performing linear fitting analysis and titer calculation on the effective data group to obtain reference data of linear fitting correlation coefficients, titer mean values and titer median values, and obtaining titer measurement results to form a change rate linear fitting graph.
2. The method of constructing an information structure based on titer detection of enzyme activity assay according to claim 1, wherein: in the step A, the kinetic parameters set by the ultraviolet-visible spectrophotometer control application software are specifically as follows: the automatic reading time interval is 5 seconds, total 330 seconds, and the data acquisition amount is 67.
3. The method of constructing an information structure based on titer detection of enzyme activity assay according to claim 1, wherein: and step C, compiling a PDF file analysis script according to a PDF report file format by using a matching script module in LabWare7.0, and capturing the original data into the system.
4. The method of constructing an information structure based on titer detection of enzyme activity assay according to claim 1, wherein: and D, creating an electronic original record, recording the original data in an original data recording module, and uploading the original map attachment to the corresponding electronic original record.
5. The method of constructing an information structure based on titer detection of enzyme activity assay according to claim 1, wherein: and step E, grouping the data points of which the data points are one every 30 seconds into data groups, using an electronic original recording standard function writing formula to judge whether the data level difference meets the specification, and if the data level difference does not meet the specification, rejecting the data groups without performing linear fitting analysis on the data groups.
6. The method of constructing an information structure based on titer detection of enzyme activity assay according to claim 1, wherein: and step F, obtaining the correlation coefficient, the titer mean value and the titer median of each group of data according to a statistical method, and displaying the titer result, the used original data and the important reference data in a test recording module.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1394234A (en) * 2000-09-06 2003-01-29 蒂莫西·A·霍奇 Systems, methods and devices for transgene and directed mutagenesis screening
CN102336816A (en) * 2011-10-09 2012-02-01 江苏省血吸虫病防治研究所 Schistosoma japonicum thioredoxin glutathione reductase (SjTGR) functional inhibitory peptides and application thereof
CN104412270A (en) * 2012-05-02 2015-03-11 迪亚肿瘤技术公司 System and method for automated determination of the relative effectiveness of anti-cancer drug candidates
CN105893340A (en) * 2016-05-23 2016-08-24 深圳翔宇信息技术有限公司 Efficient data processing system used during detection and analysis
CN107463603A (en) * 2017-06-16 2017-12-12 中国科学院计算机网络信息中心 It is a kind of that control method and system are customized based on the scientific research project life cycle data management for quantifying DMP

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101776586A (en) * 2009-01-12 2010-07-14 中国水产科学研究院东海水产研究所 Method for identifying sperm viability of large yellow croaker
CN105628636B (en) * 2015-12-24 2018-08-03 安徽瑞达健康产业有限公司 A kind of chymotrypsin vigor testing methods
US11456059B2 (en) * 2017-08-22 2022-09-27 Geospatial Technology Associates Llc System, apparatus and method for hierarchical identification, multi-tier target library processing, and two-stage identification

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1394234A (en) * 2000-09-06 2003-01-29 蒂莫西·A·霍奇 Systems, methods and devices for transgene and directed mutagenesis screening
CN102336816A (en) * 2011-10-09 2012-02-01 江苏省血吸虫病防治研究所 Schistosoma japonicum thioredoxin glutathione reductase (SjTGR) functional inhibitory peptides and application thereof
CN104412270A (en) * 2012-05-02 2015-03-11 迪亚肿瘤技术公司 System and method for automated determination of the relative effectiveness of anti-cancer drug candidates
CN105893340A (en) * 2016-05-23 2016-08-24 深圳翔宇信息技术有限公司 Efficient data processing system used during detection and analysis
CN107463603A (en) * 2017-06-16 2017-12-12 中国科学院计算机网络信息中心 It is a kind of that control method and system are customized based on the scientific research project life cycle data management for quantifying DMP

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Laboratory measurement: Chlorophyll-a concentration measurement with acetone method using spectrophotometer;F. Johan等;《2014 IEEE International Conference on Industrial Engineering and Engineering Management》;20150312;第744-748页 *
药品实验室自动化及无纸化检测的设计与实现——基于 LIMS 系统;李珺婵;《中国优秀硕士学位论文全文数据库 信息科技辑》;20170215;第2017年卷(第2期);第1.3节第2段、第2.1.1节第1段、第2.1.2节第2段、第3.2.2节第2段、第3.2.3节第1-10段、第4.2.2节第1段、第4.4.2.1第2段 *
质检实验室数据自动化处理系统的设计与研究;郑毅等;《信息化建设》;20140320;第134-135页 *

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