CN110007102A - An accurate and quantitative sampling device for pipetting - Google Patents

Abstract

The invention discloses an accurate quantitative sampling device by pipetting. The exhaust port of the liquid pipe and the connecting pipe of the pump, the two-position three-way valve connecting the digestion reactor and the multi-channel valve group, the photoelectric detector for judging whether the liquid is discharged, the valve for controlling the liquid discharge switch, the pipette and the pump. The buffer pipe between the two, the photoelectric detector for judging the on-off of the liquid at the exhaust port, the control module for automatic control of the joint work of each module, and the convergence point of the multi-channel valve group connected to the normally closed end of the two-position three-way valve.

Description

An accurate and quantitative sampling device for pipetting

technical field

The invention relates to the technical field of accurate quantitative liquid transfer, in particular to a device for accurate quantitative liquid transfer sampling.

Background technique

In the automatic combination of analytical chemistry technology and instrumentation, stable and accurate pipetting quantitative technology methods and systems are required as extended applications of laboratory analysis, such as online analyzers.

At present, the online analyzers on the market have not adopted this kind of pipetting quantitative sampling method and system equivalent to the chemical laboratory pipette operation. At the same time, the multi-port valve is susceptible to reagent contamination and errors caused by its own dead volume and liquid leakage. The traditional photoelectric metering speed is slow and time-consuming, so this injection method brings great inconvenience to the product.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide an accurate quantitative sample introduction device by pipetting, so as to solve the problems raised in the above-mentioned background art.

To achieve the above object, the present invention provides the following technical solutions:

An accurate quantitative sampling device for pipetting, including a pipette with an exhaust port and a liquid discharge port, a pump capable of forward and reverse rotation, a multi-channel valve group capable of injecting different liquids, and a pipette connected to exhaust The connecting pipe between the port and the pump, the two-position three-way valve connecting the digestion reactor and the multi-channel valve group, the photoelectric detector for judging whether the liquid is discharged or not, the valve for controlling the discharge switch, the buffer tube between the pipette and the pump , a photoelectric detector for judging the liquid on/off of the exhaust port, a control module for automatic control of the joint work of each module, and a convergence point for a multi-channel valve group connected to the normally closed end of the two-position three-way valve.

Preferably, the multi-channel valve set communicates with a first sampling channel, a second sampling channel, a third sampling channel, a fourth sampling channel and a fifth sampling channel.

The control module is respectively connected with the pump, the detector, the photoelectric detector, the valve and the two-position three-way valve.

The output end of the pump is connected to the buffer pipe.

The first sampling channel, the second sampling channel, the third sampling channel, the fourth sampling channel and the fifth sampling channel are all connected to the convergence point.

The output end of the photodetector is connected with the input end of the detector, and the output end of the detector is connected with the signal end of the two-position three-way valve.

The output end of the photodetector is connected with the signal end of the valve.

This method and system for precise quantitative injection by pipetting is used as a module or component of an online analyzer to complete the pipetting injection of water samples, volume standards, zero standards, pure water, and reagents, and is suitable for chemical laboratory pipette operations. , to avoid cross-contamination, it can perform pipetting and washing before pipetting and injection, and can also perform pipetting cleaning. The different dilution ratios of the measured water samples improve the field adaptability of the water quality online analyzer and the range of the online analyzer.

Description of drawings

Fig. 1 is the front structure schematic diagram of the present invention;

In the figure: 1-pipette, 2-pump, 31-first injection channel, 32-second injection channel, 33-third injection channel, 34-fourth injection channel, 35-fifth injection channel Sample channel, 4-2/3-way valve, 5-photoelectric detector, 6-valve, 7-control module, 8-buffer tube, 9-detector, 10-convergence point.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inside", " The orientation or positional relationship indicated by "outside" is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation, so as to The specific orientation configuration and operation are therefore not to be construed as limitations of the present invention.

Referring to FIG. 1, the present invention provides a technical solution: an accurate and quantitative sample introduction device for pipetting, including a pipette 1 with an exhaust port and a liquid discharge port, a pump 2 that can be reversible, and can achieve different Multi-channel valve group for liquid sampling, connecting pipe connecting pipette exhaust port and pump, two-position three-way valve connecting digestion reactor and multi-channel valve group 4, photoelectric detector 5 for judging liquid discharge, The valve 6 for controlling the discharge switch, the buffer tube 8 between the pipette and the pump, the detector 9 for judging the on-off of the liquid at the exhaust port, and the control module 7 for automatically controlling the joint work of each module, and the two-position three-way valve 4. The convergence point 10 of the multi-channel valve group connected to the normally closed end.

The multi-channel valve group communicates with a first sampling channel 31 , a second sampling channel 32 , a third sampling channel 33 , a fourth sampling channel 34 and a fifth sampling channel 36 .

The control module 7 is respectively connected with the pump 2 , the detector 9 , the photodetector 5 , the valve 6 and the two-position three-way valve 4 .

The output of the pump 2 is connected to a buffer pipe 8 .

The first sampling channel 31 , the second sampling channel 32 , the third sampling channel 33 , the fourth sampling channel 34 and the fifth sampling channel 36 are all connected to the convergence point 10 .

The output end of the photodetector 5 is connected to the input end of the detector 9 , and the output end of the detector 9 is connected to the signal end of the two-position three-way valve 4 .

The output end of the photodetector 5 is connected to the signal end of the valve 6

An accurate and quantitative sampling method by pipetting and a system's pipetting and rinsing process. The pipetting and rinsing operation is divided into two steps: extracting and quantifying, extracting and discharging, and extracting and quantifying through the above-mentioned precise and quantitative sampling method and system by pipetting. The module 7 controls the valve 6 to open, the normally closed end of the two-position three-way valve 4 opens, closes the valve 6, the pump 2 rotates clockwise, and the liquid will be sucked by the negative pressure through the first sampling channel 31, the second sampling channel 32, and the first sampling channel 32. The three sampling channels 33, the fourth sampling channel 34, the fifth sampling channel 35, and the two-position three-way valve 4 enter the pipette, and the liquid overflowing the pipette will enter the liquid discharge port by gravity and be detected by the photodetector 5 If it is detected, the extraction and quantitative step is completed, and the extraction and discharge of liquid is immediately switched by closing the valve of the multi-channel valve group 3a to the valve 6 opening, the second sampling channel 32 is connected to the air, and the liquid continues to overflow out of the pipette and enter through the detector 9. Buffer tube, when the detector 9 detects that there is no liquid in the liquid pipeline, the valve 6 is opened at the same time, the pump 2 rotates clockwise, the liquid passes through the buffer tube 8, the detector 9, the photoelectric detector 5, and the valve 6 is emptied until the detector 9. If the detector 9 cannot detect the liquid, stop the pump 2, that is, the pumping and discharging step is completed, and the pipetting and rinsing operation is realized.

The pipetting precise and quantitative sampling device is divided into two steps for the pipetting and sampling operation, namely, extraction and quantitative injection, and pipetting and injection. The closed end is opened, the valve 6 is closed, the pump 2 is rotated clockwise, and the liquid will be sucked by negative pressure through the first sampling channel 31, the second sampling channel 32, the third sampling channel 33, the fourth sampling channel 34, and the first sampling channel 31. Five sampling channels 35, two-position three-way valve 4, enter the pipette, the liquid overflowing the pipette will enter the liquid discharge port by gravity and be detected by the photoelectric detector 5, that is, the quantitative extraction step is completed, and the pipette injection passes through Immediately stop the pump 2, at the same time switch the 3/2-way valve 4 to the normally open end connection, open the valve 6, and force the liquid from the pipette through the low end of the pipette 1 to the common end of the 3/2-way valve 4 through the normally open end by gravity Entering the digestion reaction tank, the pipetting and sampling steps are completed, and the precise sampling operation of pipetting is realized.

An accurate and quantitative sampling method by pipetting and a system's pipetting cleaning process. The pipetting cleaning operation is divided into three steps: extracting and quantifying, extracting and cleaning, and extracting and discharging. The control module 7 controls the valve 6 (such as connecting pure water) to open, the two-position three-way valve 4 is normally open, closes the valve 6, the pump 2 rotates clockwise, and the pump 2 continues to rotate clockwise for extraction and cleaning, and the photodetector 5 detects the for a certain cleaning time, that is, the extraction and cleaning step is completed. The liquid continues to overflow out of the pipette and enter the buffer tube through the photodetector 5 and the two-position three-way valve 4 from the normally open end to the common end. When the photodetector 5 detects the liquid tube When there is no liquid in the circuit, the pump 2 rotates counterclockwise, and the liquid passes through the buffer tube 8, the two-position three-way valve 4, the exhaust port of the pipette 1 and the photoelectric detector 5, the liquid outlet of the pipette 1 and the photoelectric detector 5, The valve 6 is emptied until the photoelectric detector 5 and the photoelectric detector 5 cannot detect the liquid, and the pump 2 is stopped, that is, the extraction and drainage steps are completed, and the pipetting cleaning operation is realized. The cleaning time can be set and controlled.

The glass cover of the pipette 1 of the above-mentioned method and system for accurate and quantitative pipetting includes an exhaust port (the exhaust port on the glass cover passes through the photoelectric detector 5), and the liquid discharge port (the liquid discharge port under the glass cover passes through the photoelectric detector) 5), in the vertical direction, the liquid discharge port is lower than the exhaust port, and the exhaust port is lower than the overflow top of pipette 1. When pump 2 is forward clockwise, the liquid flows from the overflow top of pipette 1. The outflow does not touch the top of the glass cover. When the pump 2 continues to rotate clockwise, the liquid flowing out of the overflow top of the pipette 1 will pass through the exhaust port of the pipette 1 and the two-position three-way due to the closing of the valve 6. The liquid fills the space of the discharge port due to gravity. Valve 4, buffer pipe 8, and pump 2 continue to discharge. When the pump is reversed counterclockwise and the valve 6 is opened, the liquid from the buffer tube will enter the glass cover through the two-position three-way valve 4, the exhaust port of the pipette 1 and the upper photodetector 5, and will not enter the overflow of the pipette 1. The top of the flow passes through the lower photodetector 5 and enters the liquid discharge port, and the valve 6 discharges.

The above-mentioned method and system for precise quantitative sampling by pipetting can also use a drain port to discharge the cleaning waste liquid. , that is, a method of pipetting accurate quantitative sampling and the pipetting cleaning operation of the system is controlled by the control module 7 to control the valve 6 (such as connecting pure water) to open, the two-position three-way valve 4 is normally open, and the valve 6 is closed. Turn the hour hand to enter the pipette 1, the liquid overflowing into the pipette 1 will enter the discharge port by gravity and be detected by the photoelectric detector 5, that is, the quantitative extraction step is completed, and the liquid continues to overflow out of the pipette 1, and then pass the pipetting. The exhaust port of pipe 1 passes through the photodetector 5 and the two-position three-way valve 4 from the normally open end to the common end and enters the buffer pipe 8. When the photoelectric detector 5 detects that there is no liquid in the liquid pipeline, the valve 3b is closed at the same time, and the valve is opened. 6. The pump 2 rotates counterclockwise, the liquid flows from the buffer tube 8, the two-position three-way valve 4, the exhaust port of the pipette 1 and the photoelectric detector 5, the liquid discharge port of the pipette 1 and the photoelectric detector 5, the valve 6. Evacuate until the photodetector 5 and the photodetector 5 cannot detect the liquid, and stop the pump 2, that is, the extraction and drainage steps are completed, and the pipetting cleaning operation is realized. The number of times the pipetting cleaning operation is performed can be set to control the number of cleaning operations.

The above-mentioned method and system for precise quantitative sampling by pipetting can be combined with the liquid evacuated by the pump 2 and the liquid evacuated by the valve 6 and selectively collected through the two-position three-way valve, which has reduced the need for harmless treatment. To deal with the amount of waste liquid generated, the waste liquid from pipetting rinsing and pipetting cleaning can be combined and collected to be directly discharged into the environment, and the reagent waste liquid for pipetting and sampling is collected separately for harmless treatment.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, and substitutions can be made in these embodiments without departing from the principle and spirit of the invention and modifications, the scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

1. a kind of liquid relief accurate quantification sampling device, it is characterised in that: including with exhaust outlet and leakage fluid dram pipette (1), It can the pump (2) of positive and negative rotation, the multichannel valve group for being able to achieve different liquids sample introduction, connection pipette (1) exhaust outlet and the company for pumping (2) Adapter tube, the photoelectric detector (5) whether judge drain, is controlled the two-position three-way valve (4) of connection resolution reactor and multichannel valve group Separator tube (8) between the valve (6) of draining switch processed, pipette and pump (2), the detector for judging exhaust outlet liquid on-off (9) and the control module (7) of automation control each module associated working, the multi-pass that is connected with two-position three-way valve (4) normal-closed end The convergent point (10) of road valve group.

2. a kind of liquid relief accurate quantification sampling device according to claim 1, it is characterised in that: the multichannel valve group connects It is connected with the first sample intake passage (31), the second sample intake passage (32), third sample intake passage (33), the 4th sample intake passage (34) and the 5th Sample intake passage (36).

3. a kind of liquid relief accurate quantification sampling device according to claim 1, it is characterised in that: the control module (7) It is connect respectively with pump (2), detector (9), photoelectric detector (5), valve (6) and two-position three-way valve (4).

4. a kind of liquid relief accurate quantification sampling device according to claim 1, it is characterised in that: the output of the pump (2) End is connect with separator tube (8).

5. a kind of liquid relief accurate quantification sampling device according to claim 2, it is characterised in that: first sample intake passage (31), the second sample intake passage (32), third sample intake passage (33), the 4th sample intake passage (34) and the 5th sample intake passage (36) with Convergent point (10) connection.

6. a kind of liquid relief accurate quantification sampling device according to claim 1, it is characterised in that: the photoelectric detector (5) output end is connect with the input terminal of detector (9), the output end of the detector (9) and the signal of two-position three-way valve (4) End connection.

7. a kind of liquid relief accurate quantification sampling device according to claim 1, it is characterised in that: the photoelectric detector (5) output end is connect with the signal end of valve (6).