CN109998055B - Hemp seed antioxidant polypeptide chewable tablet and preparation method thereof - Google Patents
Hemp seed antioxidant polypeptide chewable tablet and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a hemp seed antioxidant polypeptide chewable tablet and a preparation method thereof, belonging to the technical field of health care products. In order to overcome the defects of complex components and low functional activity of the existing polypeptide mixture, the invention provides a hemp seed antioxidant polypeptide chewable tablet, and the raw material comprises hemp seed polypeptide P2Powder, blueberry fruit superfine powder, corn starch, microcrystalline cellulose, xylitol, magnesium stearate, malic acid and flaxseed gum. The polypeptide component P with antioxidant activity is obtained by carrying out enzymolysis, separation, purification and screening on hemp seed meal2Compared with the health care product prepared by the existing polypeptide mixture, the antioxidant polypeptide chewable tablet prepared by compounding various functional active substances and auxiliary materials is mainly used as a functional component, and the polypeptide component P2The components are single, and the antioxidant activity is stronger; the blueberry seed polypeptide chewable tablet is mixed with blueberry fruit superfine powder, so that the taste and color of the hemp seed polypeptide chewable tablet are improved, and the antioxidation and aging delaying effects of the chewable tablet are enhanced.
Description
Technical Field
The invention belongs to the technical field of health care products, and particularly relates to a hemp seed antioxidant polypeptide chewable tablet and a preparation method thereof.
Background
The hemp seeds are unique in nutrition in the aspects of grease and protein, and have been developed into functional foods or health care products in developed countries such as Europe, America, Japan, Korea and the like, while in the primary processing stage of China, the development of high value-added products is needed, and the chain of the hemp industry is further extended.
The bioactive polypeptide is generated by protein through specific enzyme hydrolysis, has the characteristics of greenness, health, easy absorption, wide source and good safety, and is more and more concerned by people due to multiple health-care effects of oxidation resistance, antibiosis and the like.
The bioactive polypeptide serving as a novel functional active factor can be widely applied to special purpose foods such as low-allergy foods, nutritional curative effect foods, exercise-enhanced foods, easily digestible and absorbable foods, functional health-care foods, baby foods, antihypertensive foods, novel fermented foods and the like, and can generate huge social benefits and economic benefits.
At present, the development of polypeptide products is still in the primary stage in China, the polypeptide products mostly stay in a polypeptide mixture after proteolysis, and the polypeptide mixture has more complex components and low extraction and purification degree, so that the functional activity of polypeptide components is difficult to ensure. At the present stage, the development of polypeptide products does not have a process of sequencing polypeptide components, and the purification and product development aiming at functional polypeptides are lacked.
Disclosure of Invention
In order to overcome the defects of complex components and low functional activity of the existing polypeptide mixture, the invention provides a hemp seed antioxidant polypeptide chewable tablet and a preparation method thereof.
The technical scheme of the invention is as follows:
a hemp seed antioxidant polypeptide chewable tablet comprises the following raw materials in parts by weightMaterial preparation: hemp seed polypeptide P215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum.
Further, the feed comprises the following raw materials in parts by weight: hemp seed polypeptide P220 parts of powder, 15 parts of blueberry fruit superfine powder, 40 parts of corn starch, 1 part of microcrystalline cellulose, 18 parts of xylitol, 1.5 parts of magnesium stearate, 0.5 part of malic acid and 2 parts of flaxseed gum.
A preparation method of hemp seed antioxidant polypeptide chewable tablets comprises the following steps:
step one, preparation of hemp seed polypeptide:
weighing hemp seed meal with a certain mass, adding distilled water and papain, carrying out enzymolysis at a certain pH value and temperature, adjusting the pH value after the enzymolysis is finished, heating to inactivate the enzyme, centrifuging the enzymolysis liquid, and collecting supernatant;
step two, separating hemp seed polypeptide:
separating the supernatant collected in the first step by a membrane filtration system, separating the mixed hemp seed polypeptide in the supernatant into 2 peptide segments with different molecular weights by a filtration membrane with the molecular weight cutoff of 1KD, selecting the hemp seed peptide segment with the molecular weight of less than 1KD for freeze-drying treatment to obtain a hemp seed polypeptide component F1Pulverizing;
step three, purifying hemp seed polypeptide:
using buffer solution to separate the hemp seed polypeptide component F obtained in the step two1Dissolving the powder to prepare a sample loading solution with a certain mass concentration, eluting by adopting chromatographic resin and using 25mM Tris-HCl +0.25M NaCl with the pH value of 8.5, 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5 and 25mM Tris-HCl +0.45M NaCl with the pH value of 8.5 as eluent, collecting the elution components of 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5, and freeze-drying to obtain the hemp seed polypeptide component P2Pulverizing;
step four, preparing the hemp seed antioxidant polypeptide chewable tablet:
weighing the hemp seed polypeptide P obtained in the third step according to the following weight parts215-25 parts of powder and ultra-micro blueberry fruit10-18 parts of powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum, and fully mixing and tabletting to obtain the hemp seed antioxidant polypeptide chewable tablet.
Further, the mass ratio of the hemp seed meal to the distilled water in the first step is 1:10, the adding amount of the papain is 0.5g of the papain per 100mL of an enzymolysis system, and the enzyme activity unit of the papain is 80 ten thousand u/g.
Further, in the first step, the pH value of enzymolysis is 5.0, the enzymolysis temperature is 70 ℃, and the enzymolysis time is 1 h.
Further, after the enzymolysis is finished, the pH value is adjusted to 7.0, the temperature for heating and enzyme deactivation is 95 ℃, the enzyme deactivation time is 15min, and the centrifugation is 10000r/min and 20 min.
Further, in the second step, the membrane filtration system is a tangential flow membrane filtration system, and the freeze-drying adopts a vacuum freeze-drying machine.
Further, in the third step, the type of the chromatography resin is Capto DEAE chromatography resin, the buffer solution is 25mM Tris-HCl with the pH value of 8.5, and the mass concentration of the loading solution is 60 mg/mL.
Further, the eluent in the step three is sequentially and respectively eluted for 6 column volumes, and the elution flow rate is 2.6 mL/min.
Further, the hemp seed polypeptide component P obtained in the third step2The molecular weight of the polypeptide is 876Da, and the amino acid sequence is Ile-Asp-Lys-Phe-Ser-Arg.
The invention has the beneficial effects that:
the hemp seed antioxidant polypeptide chewable tablet provided by the invention is prepared by carrying out enzymolysis, separation, purification and screening on hemp seed meal to obtain a polypeptide component P with antioxidant activity2The tablet is prepared by compounding various functional active substances and auxiliary materials thereof as main functional components, the product does not need a disintegration process, and the tablet is quick to dissolve, quick to absorb and high in bioavailability; convenient carrying and stable quality. In the whole process flow, the raw materials are not contacted with water, so that the raw materials are not easily polluted by microorganisms, and the stability is improved; convenient to take, and is especially suitable for swallowingDifficult patients; is a functional food which is convenient to carry and preserve and is suitable for both the old and the young, and can meet the requirement of modern high-paced urban life.
The hemp seed antioxidant polypeptide chewable tablet provided by the invention is prepared from a polypeptide component P with the molecular weight of 876Da and the amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg2Is a main functional component, compared with the health product prepared from the existing polypeptide mixture, the polypeptide component P2The components are single, and the antioxidant activity is stronger; the blueberry seed polypeptide chewable tablet is mixed with blueberry fruit superfine powder, so that the taste and color of the hemp seed polypeptide chewable tablet are improved, and the antioxidation and aging delaying effects of the chewable tablet are enhanced.
Detailed Description
The technical solutions of the present invention are further described below with reference to the following examples, but the present invention is not limited thereto, and any modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Example 1
A hemp seed antioxidant polypeptide chewable tablet comprises the following raw materials in parts by weight: hemp seed polypeptide P215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum.
Example 2
A hemp seed antioxidant polypeptide chewable tablet comprises the following raw materials in parts by weight: hemp seed polypeptide P220 parts of powder, 15 parts of blueberry fruit superfine powder, 40 parts of corn starch, 1 part of microcrystalline cellulose, 18 parts of xylitol, 1.5 parts of magnesium stearate, 0.5 part of malic acid and 2 parts of flaxseed gum.
Example 3
A hemp seed antioxidant polypeptide chewable tablet comprises the following raw materials in parts by weight: hemp seed polypeptide P217 parts of powder, 12 parts of blueberry fruit superfine powder, 37 parts of corn starch, 0.8 part of microcrystalline cellulose, 16 parts of xylitol, 1.2 parts of magnesium stearate, 0.8 part of malic acid and 4 parts of flaxseed gum.
Example 4
A hemp seed antioxidant polypeptide chewable tablet comprises the following raw materials in parts by weight: hemp seed polypeptide P223 parts of powder, 17 parts of blueberry fruit superfine powder, 42 parts of corn starch, 1.2 parts of microcrystalline cellulose, 20 parts of xylitol, 2.5 parts of magnesium stearate, 1.2 parts of malic acid and 8 parts of flaxseed gum.
Example 5
A preparation method of hemp seed antioxidant polypeptide chewable tablets comprises the following steps:
step one, preparation of hemp seed polypeptide:
weighing hemp seed meal with a certain mass, adding distilled water and papain, carrying out enzymolysis at a certain pH value and temperature, adjusting the pH value after the enzymolysis is finished, heating to inactivate the enzyme, centrifuging the enzymolysis liquid, and collecting supernatant;
step two, separating hemp seed polypeptide:
separating the supernatant collected in the first step by a membrane filtration system, separating the mixed hemp seed polypeptide in the supernatant into 2 peptide segments with different molecular weights by a filtration membrane with the molecular weight cutoff of 1KD, selecting the hemp seed peptide segment with the molecular weight of less than 1KD for freeze-drying treatment to obtain a hemp seed polypeptide component F1Pulverizing;
step three, purifying hemp seed polypeptide:
using buffer solution to separate the hemp seed polypeptide component F obtained in the step two1Dissolving the powder to prepare a sample loading solution with a certain mass concentration, eluting by adopting chromatographic resin and using 25mM Tris-HCl +0.25M NaCl with the pH value of 8.5, 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5 and 25mM Tris-HCl +0.45M NaCl with the pH value of 8.5 as eluent, collecting the elution components of 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5, and freeze-drying to obtain the hemp seed polypeptide component P2Pulverizing;
step four, preparing the hemp seed antioxidant polypeptide chewable tablet:
weighing the hemp seed polypeptide P obtained in the third step according to the following weight parts215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum, and the components are fully mixedAnd tabletting to obtain the hemp seed antioxidant polypeptide chewable tablet.
Example 6
A preparation method of hemp seed antioxidant polypeptide chewable tablets comprises the following steps:
step one, preparation of hemp seed polypeptide:
weighing hemp seed meal with a certain mass, adding distilled water according to the mass ratio of the hemp seed meal to the distilled water of 1:10, adding 0.5g of papain according to an enzymolysis system of every 100mL, wherein the unit of enzyme activity of the papain is 80 ten thousand u/g, the enzymolysis pH is 5.0, the enzymolysis temperature is 70 ℃, the enzymolysis time is 1h, adjusting the pH value to 7.0 after the enzymolysis is finished, heating to 95 ℃ to inactivate enzyme for 15min, centrifuging the enzymolysis liquid for 20min at the rotation speed of 10000r/min, and collecting supernatant.
Step two, separating hemp seed polypeptide:
separating the supernatant collected in the first step by a tangential flow membrane filtration system, separating the mixed hemp seed polypeptide in the supernatant into 2 peptide segments with different molecular weights by a filtration membrane with the molecular weight cutoff of 1KD, selecting the hemp seed peptide segment with the molecular weight less than 1KD, and performing freeze-drying treatment by a vacuum freeze-dryer to obtain the hemp seed polypeptide component F1Pulverizing;
step three, purifying hemp seed polypeptide:
using buffer solution to separate the hemp seed polypeptide component F obtained in the step two1Dissolving the powder to prepare a sample loading solution with a certain mass concentration, eluting by adopting chromatographic resin and using 25mM Tris-HCl +0.25M NaCl with the pH value of 8.5, 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5 and 25mM Tris-HCl +0.45M NaCl with the pH value of 8.5 as eluent, collecting the elution components of 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5, and freeze-drying to obtain the hemp seed polypeptide component P2Pulverizing;
step four, preparing the hemp seed antioxidant polypeptide chewable tablet:
weighing the hemp seed polypeptide P obtained in the third step according to the following weight parts215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid, 1-10 parts of flaxseed gum, and the mixture is fully mixedMixing, and tabletting to obtain antioxidant polypeptide chewable tablet.
Example 7
A preparation method of hemp seed antioxidant polypeptide chewable tablets comprises the following steps:
step one, preparation of hemp seed polypeptide:
weighing hemp seed meal with a certain mass, adding distilled water according to the mass ratio of the hemp seed meal to the distilled water of 1:10, adding 0.5g of papain according to an enzymolysis system of every 100mL, wherein the unit of enzyme activity of the papain is 80 ten thousand u/g, the enzymolysis pH is 5.0, the enzymolysis temperature is 70 ℃, the enzymolysis time is 1h, adjusting the pH value to 7.0 after the enzymolysis is finished, heating to 95 ℃ to inactivate enzyme for 15min, centrifuging the enzymolysis liquid for 20min at the rotation speed of 10000r/min, and collecting supernatant.
Step two, separating hemp seed polypeptide:
separating the supernatant collected in the first step by a tangential flow membrane filtration system, separating the mixed hemp seed polypeptide in the supernatant into 2 peptide segments with different molecular weights by a filtration membrane with the molecular weight cutoff of 1KD, selecting the hemp seed peptide segment with the molecular weight less than 1KD, and performing freeze-drying treatment by a vacuum freeze-dryer to obtain the hemp seed polypeptide component F1Pulverizing;
step three, purifying hemp seed polypeptide:
subjecting the hemp seed polypeptide fraction F obtained in step two to 25mM Tris-HCl with pH of 8.51Dissolving the powder to prepare a sample loading solution with the mass concentration of 60mg/mL, adopting Capto DEAE chromatographic resin, the specification of a chromatographic column is 2.6 multiplied by 38cm, taking 25mM Tris-HCl +0.25M NaCl with the pH value of 8.5, 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5 and 25mM Tris-HCl +0.45M NaCl with the pH value of 8.5 as eluent, sequentially and respectively eluting the three eluents for 6 column volumes at the elution flow rate of 2.6mL/min, collecting the elution components of 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5, and obtaining the hemp seed polypeptide component P after freeze-drying treatment2Pulverizing; hemp seed polypeptide component P2Has a molecular weight of 876Da and is used for hemp seed polypeptide component P2The amino acid sequence of the DNA is Ile-Asp-Lys-Phe-Ser-Arg after sequencing, and the DNA has stronger antioxidant activity.
Step four, preparing the hemp seed antioxidant polypeptide chewable tablet:
weighing the hemp seed polypeptide P obtained in the third step according to the following weight parts215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum, and fully mixing and tabletting to obtain the hemp seed antioxidant polypeptide chewable tablet.
Example 8
The difference between the embodiment and the embodiment 8 is only that the raw material formula of the hemp seed antioxidant polypeptide chewable tablet in the embodiment is as follows: hemp seed polypeptide P220 parts of powder, 15 parts of blueberry fruit superfine powder, 40 parts of corn starch, 1 part of microcrystalline cellulose, 18 parts of xylitol, 1.5 parts of magnesium stearate, 0.5 part of malic acid and 2 parts of flaxseed gum.
The hemp seed polypeptide chewable tablets prepared in the embodiment are subjected to sensory characterization and physical and chemical detection, and the results are shown in table 1;
TABLE 1
| Species of | Feature(s) |
| Sensory index | Light pink color |
| Flavor index | The hemp seed has unique faint scent, is sour, sweet and delicious, and is fine and smooth when being eaten |
| Tissue state | Smooth and complete surface, no crack, no section and uniform light pink color |
| Tablet weight | 2.0±0.05g |
| Physical and chemical indexes | Lead is less than or equal to 1mg/kg, arsenic is less than or equal to 0.5mg/kg |
| Hardness of tablet | 6kg.(mm2)-1 |
| Degree of friability | ≤0.5％ |
| Disintegration property | Meets the requirements of pharmacopoeia of 2015 edition. |
| Microbiological indicator | The total number of bacteria is less than or equal to 1000CFU/g, the coliform group is less than or equal to 30MPN/100g, and pathogenic bacteria are not detected. |
Comparing the mixed peptide obtained in the first step of the present example with the hemp seed polypeptide component F obtained in the second step1And step three, obtaining the hemp seed polypeptide component P2And the antioxidant activity of the hemp seed polypeptide chewable tablet prepared in the step four, and the measurement result is shown in table 2;
TABLE 2
As shown by comparing the data in Table 2, the polypeptide fraction P of hemp seed2Mixing with mixed peptide, and hemp seed polypeptide component F with molecular weight less than 1KD1Compared with the prior art, has stronger OH inhibition capability and O resistance2-Activity, Cu2+Reducing power and ABTS+The removing capability is improved, and the antioxidant effect of the hemp seed polypeptide chewable tablet is further improved after the compound of the removing capability and the blueberry ultra-fine powderActivity, 4.2 times of OH inhibiting ability of mixed peptide, anti-O2-3.9 times the activity of the mixed peptide, Cu2+ABTS with 2-fold reduction capability of mixed peptide+The clearance capacity is 1.4 times that of the mixed peptide.
The anti-aging effect of the hemp seed polypeptide chewable tablet prepared in the example is considered:
the experimental method comprises the following steps: in the experiment, 36 mice were randomly divided into 3 groups of 12 mice each, a young group, an aging group, and an aging administration group. Except for the young group, the other 2 groups were injected with 500mg/kg of D-galactose subcutaneously in the back of the neck every morning, and the young group was injected with an equal volume of physiological saline for 8 weeks. Modeling a mouse aging model, simultaneously starting to perform corresponding treatment on each group, and feeding a basal feed to a young group and an aging group; the aging administration group is fed with 1 Chinese hemp polypeptide chewable tablet in addition to basic feed. Mice in each group had free access to water and were fed continuously for 8 weeks, weighing once a week. After the raising is finished, measuring organ coefficients of each mouse;
the organ coefficient calculation formula is as follows:
coefficient of visceral organs%1/m2X 100; in the formula, m1The weight of the mouse organ/g; m is2Is the mouse body mass/g.
The results are shown in Table 3:
TABLE 3
The data in table 3 show that all organ coefficients of the mice in the aging group without administration of the drug show a descending trend, and all organ coefficients of the mice in the aging group fed with the hemp seed polypeptide chewable tablet are obviously higher than those of the mice in the aging group, even slightly higher than those of the mice in the young group, which shows that the hemp seed polypeptide chewable tablet prepared by the embodiment has the function of resisting oxidation, can improve the organ coefficients of animals, and plays a role in delaying the organ degeneration of the aging mice.
The effect of taking the hemp seed polypeptide chewable tablets on the T-AOC and T-SOD activity of the mice with D-galactose senescence is determined, and the result is shown in Table 4;
TABLE 4
The total antioxidant capacity T-AOC is an important index for responding to the antioxidant level, and the reduction of the T-AOC is important physiological change in the aging process of organisms and is closely related to the health of the organisms. As can be seen from the data in Table 4, the T-AOC of the liver and brain tissues of the mice in the aging group without administration of the drug is obviously reduced, and the T-AOC of the mice in the aging group fed with the hemp polypeptide chewable tablet basically maintains the same level as that of the mice in the young group, which shows that the hemp seed polypeptide chewable tablet prepared by the embodiment has stronger anti-aging effect.
The total superoxide dismutase T-SOD is also one of the important indexes for evaluating whether the organism is aged, and the data in the table 4 show that the T-SOD of the serum and the brain tissue of the aged mice which are not administrated and the aged mice which are fed with the hemp polypeptide chewable tablets are obviously reduced, but the T-SOD reduction amplitude of the aged mice which are fed with the hemp polypeptide chewable tablets is obviously smaller than that of the aged mice which are not administrated, which shows that the hemp seed polypeptide chewable tablets prepared by the embodiment can delay the aging of the organism.
Determining the influence of taking the hemp seed polypeptide chewable tablets on the MDA level in serum, liver and brain tissues of mice with D-galactose senescence, and the result is shown in Table 5;
TABLE 5
Malondialdehyde MDA is one of the end products of lipid peroxidation of cell membranes, and measuring its content can indirectly estimate the degree of lipid peroxidation. As can be seen from the data in Table 5, the MDA content in the serum, liver and brain tissues of the mice in the aging group fed with the hemp seed polypeptide chewable tablet is obviously lower than that of the mice in the non-administration aging group, which shows that the MDA level in the serum, liver and brain tissues of the mice can be reduced, the lipid peroxidation level of the organism can be inhibited, the health of the organism can be maintained, and the anti-aging effect can be achieved.
SEQUENCE LISTING
<110> Daqing division of department academy of sciences of Heilongjiang province
<120> hemp seed antioxidant polypeptide chewable tablet and preparation method thereof
<130> 1
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 6
<212> PRT
<213> hemp
<400> 1
Ile Asp Lys Phe Ser Arg
1 5
Claims (10)
1. The hemp seed antioxidant polypeptide chewable tablet is characterized by comprising the following raw materials in parts by weight: hemp seed polypeptide P215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum; the obtained hemp seed polypeptide component P2The amino acid sequence of (A) is Ile-Asp-Lys-Phe-Ser-Arg.
2. The hemp seed antioxidant polypeptide chewable tablet of claim 1, which is characterized by comprising the following raw materials in parts by weight: hemp seed polypeptide P220 parts of powder, 15 parts of blueberry fruit superfine powder, 40 parts of corn starch, 1 part of microcrystalline cellulose, 18 parts of xylitol, 1.5 parts of magnesium stearate, 0.5 part of malic acid and 2 parts of flaxseed gum.
3. A preparation method of hemp seed antioxidant polypeptide chewable tablets is characterized by comprising the following steps:
step one, preparation of hemp seed polypeptide:
weighing hemp seed meal with a certain mass, adding distilled water and papain, carrying out enzymolysis at a certain pH value and temperature, adjusting the pH value after the enzymolysis is finished, heating to inactivate the enzyme, centrifuging the enzymolysis liquid, and collecting supernatant;
step two, separating hemp seed polypeptide:
separating the supernatant collected in the first step by a membrane filtration system, separating the mixed hemp seed polypeptide in the supernatant into 2 peptide segments with different molecular weights by a filtration membrane with the molecular weight cutoff of 1KD, selecting the hemp seed peptide segment with the molecular weight of less than 1KD for freeze-drying treatment to obtain a hemp seed polypeptide component F1Pulverizing;
step three, purifying hemp seed polypeptide:
using buffer solution to separate the hemp seed polypeptide component F obtained in the step two1Dissolving the powder to prepare a sample loading solution with a certain mass concentration, eluting by adopting chromatographic resin and using 25mM Tris-HCl +0.25M NaCl with the pH value of 8.5, 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5 and 25mM Tris-HCl +0.45M NaCl with the pH value of 8.5 as eluent, collecting the elution components of 25mM Tris-HCl +0.35M NaCl with the pH value of 8.5, and freeze-drying to obtain the hemp seed polypeptide component P2Pulverizing;
step four, preparing the hemp seed antioxidant polypeptide chewable tablet:
weighing the hemp seed polypeptide P obtained in the third step according to the following weight parts215-25 parts of powder, 10-18 parts of blueberry fruit superfine powder, 35-45 parts of corn starch, 0.1-1.5 parts of microcrystalline cellulose, 15-22 parts of xylitol, 1-3 parts of magnesium stearate, 0.1-1.5 parts of malic acid and 1-10 parts of flaxseed gum, and fully mixing and tabletting to obtain the hemp seed antioxidant polypeptide chewable tablets; the obtained hemp seed polypeptide component P2The amino acid sequence of (A) is Ile-Asp-Lys-Phe-Ser-Arg.
4. The preparation method of the hemp seed antioxidant polypeptide chewable tablet of claim 3, wherein in step one, the mass ratio of the hemp seed meal to the distilled water is 1:10, 0.5g of papain is added into an enzymolysis system with the addition amount of the papain being 100mL, and the enzyme activity unit of the papain is 80 wu/g.
5. The method for preparing the hemp seed antioxidant polypeptide chewable tablet according to claim 3 or 4, wherein in the first step, the enzymolysis pH is 5.0, the enzymolysis temperature is 70 ℃, and the enzymolysis time is 1 h.
6. The method for preparing the hemp seed antioxidant polypeptide chewable tablet of claim 5, wherein the pH value is adjusted to 7.0 after the completion of the enzymolysis in the step one, the temperature for raising the temperature and inactivating the enzyme is 95 ℃, the time for inactivating the enzyme is 15min, and the centrifugation is 10000r/min and 20 min.
7. The method for preparing the hemp seed antioxidant polypeptide chewable tablet of claim 6, wherein in step two the membrane filtration system is a tangential flow membrane filtration system, and the lyophilization employs a vacuum freeze dryer.
8. The method for preparing the hemp seed antioxidant polypeptide chewable tablet of claim 7, wherein the chromatography resin of step three is Capto DEAE chromatography resin, the buffer solution is 25mM Tris-HCl with pH of 8.5, and the loading solution has a mass concentration of 60 mg/mL.
9. The method for preparing the hemp seed antioxidant polypeptide chewable tablet of claim 8, wherein the eluents of step three elute 6 column volumes sequentially and respectively at an elution flow rate of 2.6 mL/min.
10. The method for preparing hemp seed antioxidant polypeptide chewable tablets of claim 9, wherein hemp seed polypeptide component P obtained in step three2Has a molecular weight of 876 Da.
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