CN109970536A - A kind of extracting method of rhizoma zingiberis effective component - Google Patents
A kind of extracting method of rhizoma zingiberis effective component Download PDFInfo
- Publication number
- CN109970536A CN109970536A CN201910325173.3A CN201910325173A CN109970536A CN 109970536 A CN109970536 A CN 109970536A CN 201910325173 A CN201910325173 A CN 201910325173A CN 109970536 A CN109970536 A CN 109970536A
- Authority
- CN
- China
- Prior art keywords
- rhizoma zingiberis
- gingerol
- effective component
- extracting method
- shine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 26
- NLDDIKRKFXEWBK-AWEZNQCLSA-N gingerol Chemical compound CCCCC[C@H](O)CC(=O)CCC1=CC=C(O)C(OC)=C1 NLDDIKRKFXEWBK-AWEZNQCLSA-N 0.000 claims abstract description 26
- JZLXEKNVCWMYHI-UHFFFAOYSA-N gingerol Natural products CCCCC(O)CC(=O)CCC1=CC=C(O)C(OC)=C1 JZLXEKNVCWMYHI-UHFFFAOYSA-N 0.000 claims abstract description 26
- 235000002780 gingerol Nutrition 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000010025 steaming Methods 0.000 claims abstract description 11
- 235000019441 ethanol Nutrition 0.000 claims abstract description 8
- SRCZQMGIVIYBBJ-UHFFFAOYSA-N ethoxyethane;ethyl acetate Chemical compound CCOCC.CCOC(C)=O SRCZQMGIVIYBBJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000003208 petroleum Substances 0.000 claims abstract description 8
- 238000004440 column chromatography Methods 0.000 claims abstract description 7
- 241000234314 Zingiber Species 0.000 claims description 16
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 16
- 235000008397 ginger Nutrition 0.000 claims description 16
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 3
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 3
- 239000011425 bamboo Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 238000010828 elution Methods 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 238000009210 therapy by ultrasound Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- YKPUWZUDDOIDPM-SOFGYWHQSA-N capsaicin Chemical compound COC1=CC(CNC(=O)CCCC\C=C\C(C)C)=CC=C1O YKPUWZUDDOIDPM-SOFGYWHQSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 208000006820 Arthralgia Diseases 0.000 description 1
- 241000050051 Chelone glabra Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000000489 anti-atherogenic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002504 capsaicin Drugs 0.000 description 1
- 235000017663 capsaicin Nutrition 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 235000020965 cold beverage Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 210000001034 respiratory center Anatomy 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000001256 steam distillation Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000001457 vasomotor Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/78—Separation; Purification; Stabilisation; Use of additives
- C07C45/79—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of extracting methods of rhizoma zingiberis effective component, include the following steps: that nine steamings nine is taken to shine rhizoma zingiberis, the ethyl alcohol that mass fraction is 80% is added, it is ultrasonically treated at least 30min, obtains gingerol medicinal extract, the effective component of gingerol is separated in a manner of column chromatography, using petroleum ether-ethyl acetate system as mobile phase, using gradient elution, petroleum ether-ethyl acetate system gradient is 7:1,3:1,1:1 (v/v), recycles mobile phase using the method for vacuum rotary steam.Method uses nine steamings nine to shine rhizoma zingiberis for raw material, first obtains gingerol medicinal extract using ultrasonic treatment, then extract the effective component in gingerol using column chromatography, and extracted rhizoma zingiberis main component is mainly 6-gingerol, and this method is simple and easy, is suitable for promoting.
Description
Technical field
The invention belongs to technical field of plant extraction, are related to a kind of extracting method of rhizoma zingiberis effective component.
Background technique
The medical value of ginger is big, and range is wide, and ginger is medicinal to divide fresh ginger, rhizoma zingiberis and bubble ginger；Rhizoma zingiberis also known as old ginger piece etc. are
Clean impurity slice dries the perennial root of ginger after digging out or small fire is dried." middle benefit gas returns positive first key medicine " is known as by Chinese medicine.
Rhizoma zingiberis property heat and it is partially dry, with warming spleen and stomach for dispelling cold, recover Yang and smooth venation, it is eliminating dampness and eliminating phlegm based on, can keep can walk, therefore centering coke cold-evil wins and simultaneous partially
Wet person and breathing with cough for cold drink volt lung are especially suitable for；Again because power speed due to act on it is stronger, therefore be used for Hui Yangfu arteries and veins, effect it is favourable.Ginger
Capsaicin is one of the active skull cap components being rich in ginger, and gingerol not only has the especially fragrant peppery flavor of ginger, but also has
There are wind dispelling, sweating, treatment cold.American scientist is research shows that " natural member " gingerol can promote blood to follow
Ring, anti-inflammatory, analgesia, are especially significantly improved arthralgia.Gingerol has vasomotor center and respiratory center bright
Aobvious excitation, also has reducing blood lipid and study of anti-atherogenic effect.
The processing method that gingerol is produced currently with ginger mainly have steam distillation, solvent extraction method, squeezing method,
Supercritical ultrasonics technology, extraction etc., the present invention provide a kind of extracting method of new rhizoma zingiberis effective component.
Summary of the invention
The purpose of the present invention is to provide a kind of extracting method of rhizoma zingiberis effective component, the rhizoma zingiberis steams nine using nine and shines
Rhizoma zingiberis.
The technical solution adopted by the invention is as follows:
A kind of extracting method of rhizoma zingiberis effective component, includes the following steps:
It takes nine steamings nine to shine rhizoma zingiberis, the ethyl alcohol that mass fraction is 80% is added, be ultrasonically treated at least 30min, obtain gingerol leaching
Cream separates the effective component of gingerol, using petroleum ether-ethyl acetate system as mobile phase, using ladder in a manner of column chromatography
Degree elution, petroleum ether-ethyl acetate system gradient are 7:1,3:1,1:1 (v/v), recycle flowing using the method for vacuum rotary steam
Phase.
In above-mentioned technical proposal, nine steamings, the nine solarization rhizoma zingiberis can be used following method and simply be made:
Storage 2 years or more old mother ginger is cleaned removal ginger peel slice and is placed on bamboo drawer and steams 50min, taking-up is placed on
60min is dried at 60 DEG C.
Further, the dosage for the ethyl alcohol that the mass fraction is 80% is 10 times that rhizoma zingiberis is shone in nine steamings nine.
The beneficial effects of the present invention are:
Extracting method of the invention uses nine steamings nine to shine rhizoma zingiberis for raw material, first obtains gingerol medicinal extract using ultrasonic treatment,
The effective component in gingerol is extracted using column chromatography again, extracted rhizoma zingiberis main component is mainly 6-gingerol, this method
It is simple and easy, it is suitable for promoting.
Specific embodiment
With specific example, the present invention will be further described below.
It takes nine steamings nine to shine rhizoma zingiberis, the ethyl alcohol that the mass fraction of 10 times of its quality is 80% is added, is ultrasonically treated 30min, obtains
Gingerol medicinal extract separates the effective component of gingerol in a manner of column chromatography, is flowing with petroleum ether-ethyl acetate system
Phase, and by the way of gradient elution, it is respectively 7:1,3:1,1:1 (v/v) time-division in petroleum ether-ethyl acetate system gradient
From through thin-layer chromatography experiments have shown that gingerol substance main component 6-gingerol is present under 3:1 gradient in elution gained liquid.
And under eluting it is the dilution of effective component, wherein mostly mobile phase, therefore the method for using vacuum rotary steam recycles
The concentration of effective component had not only can be improved in mobile phase, but also can reduce or remit mobile phase loss；
Raw material used by the method for the present invention is that rhizoma zingiberis are shone in nine steamings nine, is also possible to nine steaming nine obtained with the following method
The substitute of rhizoma zingiberis is shone, i.e., storage 2 years or more old mother ginger is cleaned removal ginger peel slice and is placed on bamboo drawer and steam 50min, taken out
It is placed on the rhizoma zingiberis that continuous drying 60min is obtained at 60 DEG C.
By the study found that having extremely significant influence to 6-gingerol yield during ultrasound obtains gingerol medicinal extract
Each factor is liquid-to-solid ratio > ultrasonic time > concentration of alcohol on the size order that 6-gingerol yield influences；Concentration of alcohol and liquid-to-solid ratio
Influence of the reciprocation to 6-gingerol yield it is the most significant, utilized from gingerol medicinal extract column chromatography method separation effectively at
Timesharing is chromatographed using multiple column, is tested with thin layer chromatography, only detected 6-gingerol in 3:1 gradient, and other
Do not have in gradient.To sum up the present invention obtains the optimum extraction process of rhizoma zingiberis effective component by series of studies.
Claims (3)
1. a kind of extracting method of rhizoma zingiberis effective component, which comprises the steps of:
It takes nine steamings nine to shine rhizoma zingiberis, the ethyl alcohol that mass fraction is 80% is added, be ultrasonically treated at least 30min, obtain gingerol medicinal extract, with
The mode of column chromatography is washed using petroleum ether-ethyl acetate system as mobile phase using gradient to separate the effective component of gingerol
De-, petroleum ether-ethyl acetate system gradient is 7:1,3:1,1:1 (v/v), recycles mobile phase using the method for vacuum rotary steam.
2. the extracting method of rhizoma zingiberis effective component according to claim 1, which is characterized in that described nine steam nine and shine rhizoma zingiberis
Following method can be used to be made:
Storage 2 years or more old mother ginger is cleaned removal ginger peel slice and is placed on bamboo drawer and steams 50min, taking-up is placed on 60 DEG C
Lower continuous drying 60min.
3. the extracting method of rhizoma zingiberis effective component according to claim 1, which is characterized in that the mass fraction is
The dosage of 80% ethyl alcohol is nine 10 times for steaming nine solarization rhizoma zingiberis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910325173.3A CN109970536A (en) | 2019-04-22 | 2019-04-22 | A kind of extracting method of rhizoma zingiberis effective component |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910325173.3A CN109970536A (en) | 2019-04-22 | 2019-04-22 | A kind of extracting method of rhizoma zingiberis effective component |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109970536A true CN109970536A (en) | 2019-07-05 |
Family
ID=67085657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910325173.3A Pending CN109970536A (en) | 2019-04-22 | 2019-04-22 | A kind of extracting method of rhizoma zingiberis effective component |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109970536A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110801503A (en) * | 2019-09-25 | 2020-02-18 | 温州市人民医院 | Dried ginger capsule and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101085727A (en) * | 2007-07-05 | 2007-12-12 | 中国人民解放军第四一一医院 | Method for preparing 6-gingerol and 8-gingerol from ginger |
| CN102091314A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Composition of dried ginger and evodia rutaecarpa, preparation method thereof and application thereof in preparing attenuating synergistic medicament in radiotherapy and chemotherapy of cancer |
| CN102976909A (en) * | 2012-12-19 | 2013-03-20 | 中国科学院西北高原生物研究所 | Method for extracting and purifying 6-gingerol from ginger |
| CN103479987A (en) * | 2013-10-07 | 2014-01-01 | 大连杰信生物科技有限公司 | Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger |
| CN105942317A (en) * | 2016-04-29 | 2016-09-21 | 安徽俊达食品有限公司 | Production technology of pure ginger slices |
| CN107298642A (en) * | 2017-07-19 | 2017-10-27 | 重庆大学 | A kind of method for extraction and purification of 6 salad oil |
-
2019
- 2019-04-22 CN CN201910325173.3A patent/CN109970536A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101085727A (en) * | 2007-07-05 | 2007-12-12 | 中国人民解放军第四一一医院 | Method for preparing 6-gingerol and 8-gingerol from ginger |
| CN102091314A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Composition of dried ginger and evodia rutaecarpa, preparation method thereof and application thereof in preparing attenuating synergistic medicament in radiotherapy and chemotherapy of cancer |
| CN102976909A (en) * | 2012-12-19 | 2013-03-20 | 中国科学院西北高原生物研究所 | Method for extracting and purifying 6-gingerol from ginger |
| CN103479987A (en) * | 2013-10-07 | 2014-01-01 | 大连杰信生物科技有限公司 | Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger |
| CN105942317A (en) * | 2016-04-29 | 2016-09-21 | 安徽俊达食品有限公司 | Production technology of pure ginger slices |
| CN107298642A (en) * | 2017-07-19 | 2017-10-27 | 重庆大学 | A kind of method for extraction and purification of 6 salad oil |
Non-Patent Citations (1)
| Title |
|---|
| 叶定江等主编: "《中药炮制学辞典》", 30 June 2005, 上海科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110801503A (en) * | 2019-09-25 | 2020-02-18 | 温州市人民医院 | Dried ginger capsule and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100590109C (en) | Extraction Technology of Active Components of Herb Cistanche | |
| CN102453601B (en) | Method for Extracting Camphor Seed Oil with Ultrasonic Assisted Composite Enzyme | |
| CN105294876B (en) | A kind of method that polysaccharide and gingerol are extracted in the accessory substance CORTEX ZINGIBERIS RHIZOMAE from zingiber processes | |
| CN106490608A (en) | A kind of Herba Dendrobii enhancing immunity oral liquid, electuary, capsule, tablet | |
| CN105901719A (en) | Donkey-hide gelatin with effects of invigorating qi, nourishing blood, beautifying face and losing weight and preparation method thereof | |
| CN104082725B (en) | A kind of snake gall honey freeze-dried powder and preparation method thereof | |
| KR101341755B1 (en) | The effective isolation method of panaxadiol saponin fraction from ginseng using pectinase | |
| CN113831230B (en) | Method for extracting and separating zingerone from ginger | |
| CN109970536A (en) | A kind of extracting method of rhizoma zingiberis effective component | |
| CN106867824A (en) | Male silkworm essence wine and preparation method thereof | |
| CN109134685A (en) | A method of extracting polysaccharide from beautiful millettia root | |
| CN105815703A (en) | Method for processing trichosanthes peel, cortex albiziae and hawthorn fruit healthcare powder | |
| CN106377567A (en) | Extraction method for small molecule active substances of traditional Chinese medicine material | |
| CN106317249A (en) | Extraction and purification method of garlic polysaccharide | |
| CN104004048A (en) | High-utilization-rate extraction and processing method of wrinkled papaya | |
| CN108157964A (en) | The preparation method of component for reducing blood fat in a kind of Laoshan celery | |
| CN105061281B (en) | A kind of method that ultrasonic wave added counter-current extraction extracts carrotene | |
| CN108969580B (en) | Preparation method and application of total tannin of blue cloth | |
| CN104173510B (en) | A kind of preparation method of Maxingshigan oral liquid | |
| CN105884925A (en) | Pumpkin peel polysaccharide and preparation method and application thereof | |
| CN110305734A (en) | A kind of extraction process of root of Dahurain angelica leaf volatile oil | |
| CN110772549A (en) | Preparation method of brucea javanica total antitumor new component | |
| CN105237608B (en) | Method for extracting hederacoside from ivy | |
| CN107041903B (en) | Integrated new method for processing and concocting radix Polygoni Multiflori Preparata in producing area | |
| CN103897951B (en) | One class kostelezkya virginica is dried leaf extract Processing wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190705 |