CN109846113A - One kind can be from the nitric oxide production mouth and nose amenities of offer - Google Patents
One kind can be from the nitric oxide production mouth and nose amenities of offer Download PDFInfo
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- CN109846113A CN109846113A CN201811445474.1A CN201811445474A CN109846113A CN 109846113 A CN109846113 A CN 109846113A CN 201811445474 A CN201811445474 A CN 201811445474A CN 109846113 A CN109846113 A CN 109846113A
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- nitric oxide
- nose
- amenities
- oxide donors
- mouth
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- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 title claims abstract description 222
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 27
- 239000002840 nitric oxide donor Substances 0.000 claims abstract description 91
- 239000000463 material Substances 0.000 claims abstract description 62
- 239000007787 solid Substances 0.000 claims abstract description 38
- 239000000022 bacteriostatic agent Substances 0.000 claims abstract description 35
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 25
- 239000000758 substrate Substances 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000004744 fabric Substances 0.000 claims description 26
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 19
- 230000004927 fusion Effects 0.000 claims description 19
- 239000007921 spray Substances 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 14
- HYHSBSXUHZOYLX-WDSKDSINSA-N S-nitrosoglutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CSN=O)C(=O)NCC(O)=O HYHSBSXUHZOYLX-WDSKDSINSA-N 0.000 claims description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical group C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 12
- -1 azo alkene Chemical class 0.000 claims description 10
- ZIIQCSMRQKCOCT-YFKPBYRVSA-N S-nitroso-N-acetyl-D-penicillamine Chemical group CC(=O)N[C@@H](C(O)=O)C(C)(C)SN=O ZIIQCSMRQKCOCT-YFKPBYRVSA-N 0.000 claims description 9
- 239000003960 organic solvent Substances 0.000 claims description 9
- 229960003180 glutathione Drugs 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- 235000010323 ascorbic acid Nutrition 0.000 claims description 6
- 229960005070 ascorbic acid Drugs 0.000 claims description 6
- 239000011668 ascorbic acid Substances 0.000 claims description 6
- 238000001548 drop coating Methods 0.000 claims description 6
- 230000001629 suppression Effects 0.000 claims description 6
- 230000008961 swelling Effects 0.000 claims description 6
- 108010024636 Glutathione Proteins 0.000 claims description 5
- 108010001742 S-Nitrosoglutathione Proteins 0.000 claims description 5
- 150000001298 alcohols Chemical class 0.000 claims description 5
- 229960004106 citric acid Drugs 0.000 claims description 5
- 235000015165 citric acid Nutrition 0.000 claims description 5
- 239000002068 microbial inoculum Substances 0.000 claims description 4
- 230000003647 oxidation Effects 0.000 claims description 4
- 238000007254 oxidation reaction Methods 0.000 claims description 4
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 4
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 238000007385 chemical modification Methods 0.000 claims description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 3
- 229960002433 cysteine Drugs 0.000 claims description 3
- 235000018417 cysteine Nutrition 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000003969 glutathione Nutrition 0.000 claims description 2
- 238000001802 infusion Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims 1
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 25
- 241000894006 Bacteria Species 0.000 abstract description 17
- 238000013268 sustained release Methods 0.000 abstract description 8
- 239000012730 sustained-release form Substances 0.000 abstract description 8
- 238000000354 decomposition reaction Methods 0.000 abstract description 7
- 230000003115 biocidal effect Effects 0.000 abstract description 5
- 230000000241 respiratory effect Effects 0.000 abstract description 4
- 206010028748 Nasal obstruction Diseases 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 230000005855 radiation Effects 0.000 abstract description 3
- 229960003753 nitric oxide Drugs 0.000 description 35
- 210000001331 nose Anatomy 0.000 description 25
- 239000000243 solution Substances 0.000 description 18
- 239000007789 gas Substances 0.000 description 14
- 238000010276 construction Methods 0.000 description 12
- 239000003795 chemical substances by application Substances 0.000 description 9
- 230000029058 respiratory gaseous exchange Effects 0.000 description 9
- 235000019441 ethanol Nutrition 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000001408 fungistatic effect Effects 0.000 description 5
- 239000011229 interlayer Substances 0.000 description 5
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 4
- 229910001431 copper ion Inorganic materials 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000002459 sustained effect Effects 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
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- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 238000007711 solidification Methods 0.000 description 3
- 230000008023 solidification Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000012237 artificial material Substances 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 210000003928 nasal cavity Anatomy 0.000 description 2
- 239000005445 natural material Substances 0.000 description 2
- 235000019391 nitrogen oxide Nutrition 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108010078321 Guanylate Cyclase Proteins 0.000 description 1
- 102000014469 Guanylate cyclase Human genes 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000003421 catalytic decomposition reaction Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- ZOOGRGPOEVQQDX-KHLHZJAASA-N cyclic guanosine monophosphate Chemical compound C([C@H]1O2)O[P@](O)(=O)O[C@@H]1[C@H](O)[C@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-KHLHZJAASA-N 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000066 endothelium dependent relaxing factor Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000027928 long-term synaptic potentiation Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000002464 muscle smooth vascular Anatomy 0.000 description 1
- 210000001640 nerve ending Anatomy 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000002856 peripheral neuron Anatomy 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides one kind can be from the nitric oxide production mouth and nose amenities of offer, the mouth and nose amenities includes base material and can be from nitric oxide production solid bacteriostatic agent is provided, which is the mixture of nitric oxide donors or nitric oxide donors and reducing agent；The solid bacteriostatic agent is supported on substrate material surface, or is encapsulated in inside base material.The mouth and nose amenities can be mask, nasal obstruction etc., when being used for nose and mouth, the steam or solar radiation contained in characteristics of contaminated respiratory droplets gas can excite nitric oxide donors to generate endogenous and the nitric oxide gas with bactericidal effect, and with certain concentration sustained release during use, effectively kill the existing bacterium of surface of sanitary, established bacterial biof iotalm is destroyed, while forming antibiotic layer in surface of sanitary.When solid bacteriostatic agent further includes reducing agent, reducing agent can further excite the decomposition of nitric oxide donors, accelerate nitric oxide donors and decompose the rate for generating NO.
Description
Technical field
The present invention, which provides one kind, to belong to medical and hygiene article field from nitric oxide production mouth and nose amenities is provided.
Background technique
Filtering enters mouth and nose air or cleans the amenities of nasal cavity, such as mask, be worn over nose and mouth for filter into
Enter the air of mouth and nose, to reach the apparatus of gas, smell, droplet disengaging wearer's mouth and nose of blocking harmful, with gauze or paper etc.
It is made.Mask itself does not have anti-(suppression) bacterium function of initiative.The layer structure of mask often gathers many outside airs
In the dirts such as dust, bacterium, microorganism, and inner layer blocks the bacterium of exhalation, saliva.Therefore, two sides cannot be used alternatingly,
Otherwise the dirt that outer layer is infected with can be sucked when abutting directly on face to human body, and become the infection sources.If the heat that mask is breathed out
Gas or saliva are got wet, and the effect of barrier germ also will be greatly reduced.
Nitric oxide is widely present in the various tissues of human body and cell, has important physiological function.Nitric oxide is again
Claim " endothelium-derived relaxing factor ", can relax vascular smooth muscle, and inhibit platelet aggregation, adjust blood pressure, prevent thrombosis.
In addition, nitric oxide can act on such as prominent preceding nerve endings of adjacent peripheral neurons and starlike colloid is thin by diffusion
Born of the same parents, reactivation guanylate cyclase such as induce and study, memory to improve cyclic guanosine monophosphate level to generate physiological effect
Related long term potentiation.
Nitric oxide is also a kind of effective broad spectrum antibiotic and fungal agents simultaneously.Normal Nasal Epithelial Cells can
High concentration nitric oxide is generated to prevent respiratory tract infection.The nitric oxide and ONOO- that macrophage generates can be killed a variety of
Pathogen, including common medical instrument infect relevant Escherichia coli and staphylococcus aureus.Studies have shown that the one of nM rank
Nitrogen oxide is just enough effectively to destroy the biomembrane that a variety of bacterial strains are formed, and the high molecular material containing nitric oxide donors can also be with
Effectively control bacterium infection.Since nitric oxide is the hydroxyl radical gas molecule of half-life period extremely short (3~5s), in mechanism not
It is also easy to produce drug resistance and toxic side effect, still, the property of nitric oxide itself gas and half-life short is unfavorable for storage and reality
It uses, this makes nitric oxide be very limited as the application of amenities bacteriostatic agent or antibacterial agent.
Summary of the invention
Goal of the invention: it is unfavorable for storing and actually using, be difficult to be used as amenities bacteriostatic agent or resist for nitric oxide
The problem of microbial inoculum, the present invention provides one kind can be from the nitric oxide production mouth and nose amenities of offer.
Technical solution: it is of the present invention can from providing nitric oxide production mouth and nose amenities, including base material and
Can be from nitric oxide production solid bacteriostatic agent be provided, which is nitric oxide donors or nitric oxide donors and reduction
The mixture of agent；Solid bacteriostatic agent is supported on substrate material surface, or is encapsulated in inside base material, forms clip type knot
Structure.
It can be mask, nasal obstruction etc. by the mouth and nose amenities, when being used for nose and mouth, contain in characteristics of contaminated respiratory droplets gas
Steam or solar radiation can excite nitric oxide donors to generate endogenous and the nitric oxide gas with bactericidal effect, and with one
It is raw to destroy established bacterium to fixed concentration for sustained release, effective killing existing bacterium of surface of sanitary during use
Object film, while antibiotic layer is formed in surface of sanitary.When solid bacteriostatic agent further includes reducing agent, reducing agent can further swash
The decomposition of nitric oxide donors is sent out, accelerates nitric oxide donors and decomposes the rate for generating NO.
Preferably, nitric oxide donors are selected from s-nitroso-N-acetylpenicillamine, s-nitrosoglutathione, azo alkene
One or more of father-in-law's glycol series compound, azo alkene father-in-law's glycol carried polymer.Base material can be natural or artificial
Material, including fiber felt pan, high-molecular fabric, flannelette, fusion spray cloth, cellulose membrane etc..
In terms of the area of basis material, when nitric oxide donors select s-nitroso-N-acetylpenicillamine, load capacity
Or the dosage being encapsulated in inside basis material is preferably equal to or greater than 6.3mg/cm2；When nitric oxide donors select S-nitrosoglutathione paddy
When the sweet peptide of Guang, load capacity or the dosage being encapsulated in inside basis material are preferably equal to or greater than 10.6mg/cm2.At this point, an oxygen
Change release nitric oxide production amount of the nitrogen donor within the unit time and release is held time can satisfy mouth and nose amenities and make
With while, it may have excellent fungistatic effect, fungistatic effect is better than common anti-biotic materials such as conventional silver ions or copper ion
Fungistatic effect.
When solid bacteriostatic agent is nitric oxide donors, the method on basis material is loaded to are as follows: by nitric oxide
Donor is dissolved in organic solvent or water, and nitric oxide donors solution is made, and then passes through solution swelling method, spraying, drop coating, leaching
Stain method loads nitric oxide donors in substrate material surface；Or nitric oxide donors are fixed on substrate material by chemical modification
On material.Preferably, organic solvent is tetrahydrofuran or alcohols；Further, alcohols can be ethyl alcohol or isopropanol.
When solid bacteriostatic agent is the mixture of nitric oxide donors and reducing agent, the side on basis material is loaded to
Method are as follows: nitric oxide donors and reducing agent mixture are obtained into solid bacteriostatic agent, solid bacteriostatic agent is then dissolved in organic solvent
In, substrate material surface is supported on by solution swelling method, drop coating, infusion process, obtains the substrate material that load has solid bacteriostatic agent
Material.Preferably, in solid bacteriostatic agent, the molar ratio of nitric oxide donors and reducing agent is 1:0.1~10.Reducing agent can be paddy Guang
One or more of sweet peptide, citric acid, ascorbic acid, cysteine.
The utility model has the advantages that compared with the prior art, the advantages of the present invention are as follows: (1) mouth and nose amenities of the invention can be certainly
Nitric oxide is provided, to have the function of initiative bactericidal, during use, to the microorganisms such as bacterium in air and exhalation
The bacterium carried in gas all has wide spectrum suppression and kills effect, securely and reliably, can be used for profession protection in routine use and hospital；(2)
Need to be with exciting agent simultaneously using just can be compared with provide nitric oxide with nitric oxide donors in the prior art, the present invention is by an oxygen
Change nitrogen donor directly to load on base material, nitric oxide production sustained release can be realized without adding other substances or exciting agent；
Mouth and nose amenities of the invention in dry conditions, can save steadily in the long term, and under human body respiration or illumination condition, one
Nitric oxide donor can be sustained nitric oxide gas, reach 4 × 10-10mol·cm-2·min-1Above concentration, and can persistently release
It puts 5 hours or more；(3) by combining reducing agent to use on the basis of nitric oxide donors, an oxidation can be accelerated to a certain degree
The decomposition rate of nitrogen donor can enable the NO in amenities when remaining long by controlling the type and ratio of reducing agent
Between discharge on the basis of discharge more quickly.
Detailed description of the invention
Fig. 1 is the release conditions that NO donor flannelette NO under the conditions of simulated respiration is loaded in embodiment 1, wherein (A) is NO
And NO2Concentration levels；It (B) is NO Flux variation tendency；
Fig. 2 is to load different NO for the sample drawing of the flannelette material of the scale of construction in embodiment 2；
Fig. 3 be different NO are loaded in embodiment 2 for the scale of construction flannelette material to the inhibitory effect figures of Escherichia coli；
Fig. 4 is that the fusion spray cloth that different bacteriostatic agents and difference NO are loaded in embodiment 3 for the scale of construction imitates the inhibition of Escherichia coli
Fruit figure；
Antibacterial effect figure when sandwich type structure is made for nitric oxide donors in embodiment 4 by Fig. 5；
Fig. 6 is in embodiment 5, and NO under the conditions of simulating human body respiration of the solid bacteriostatic agent comprising different reducing agents discharges feelings
Condition.
Specific embodiment
Technical solution of the present invention is described further with reference to the accompanying drawing.
Mouth and nose amenities refers to that filtering enters mouth and nose air or cleans the amenities, such as mask, nasal obstruction etc. of nasal cavity,
Of the invention can have following two technical solution from nitric oxide production mouth and nose amenities is provided.
The first technical solution:
It can include base material from nitric oxide production mouth and nose amenities is provided, substrate material surface load has nitric oxide
Donor or nitric oxide donors are encapsulated in inside base material, form clip type structure.Wherein, substrate material surface loads
The method of nitric oxide donors specifically: nitric oxide donors are dissolved in organic solvent or water, nitric oxide donors are made
Then solution loads nitric oxide donors in substrate material surface by the methods of solution swelling method, drop coating, dipping；Or it will
Nitric oxide donors are fixed on base material by chemical modification.Organic solvent can be tetrahydrofuran or alcohols, such as ethyl alcohol or
Isopropanol.
Second of technical solution are as follows:
The nitric oxide production mouth and nose amenities of offer it can include base material and can be from the nitric oxide production solid of offer certainly
Bacteriostatic agent.The solid bacteriostatic agent includes nitric oxide donors and reducing agent, and the molar ratio of the two is 1:0.1~10；Wherein, it restores
Agent can be glutathione, citric acid, ascorbic acid and cysteine.
Solid bacteriostatic agent can be supported on substrate material surface, or be encapsulated in inside base material, form clip type structure.
The method that solid bacteriostatic agent is supported on substrate material surface are as follows: it is antibacterial that nitric oxide donors and reducing agent mixture are obtained into solid
Agent is dissolved by organic solvent, is then supported on substrate material surface by the methods of solution swelling method, drop coating, dipping, is obtained
Load has the base material of solid bacteriostatic agent.Organic solvent can be tetrahydrofuran or alcohols, such as ethyl alcohol or isopropanol.
In above-mentioned two technical solution, nitric oxide donors can be s-nitroso-N-acetylpenicillamine (SNAP), S- nitrous
Base glutathione (GSNO), azo alkene father-in-law's glycol series compound (DBHD-NONO etc.), azo alkene father-in-law's glycol carried polymer
One or more of (PEI-NONO etc.)；Base material can be knitted for natural or artificial material, including fiber felt pan, macromolecule
Object, flannelette, fusion spray cloth, cellulose membrane etc..
In dry conditions, mouth and nose amenities of the invention can save steadily in the long term, and by the mouth and nose amenities
For nose and mouth, the steam or solar radiation contained in characteristics of contaminated respiratory droplets gas can excite nitric oxide donors sustained release endogenous and tool
There is a nitric oxide gas of bactericidal effect, and with certain concentration sustained release during use, effectively kills amenities table
The existing bacterium in face destroys established bacterial biof iotalm, while forming antibiotic layer in surface of sanitary.
Nitric oxide donors release nitric oxide usually require just to be able to achieve under given conditions, as reducing agent, illumination or
Under acid condition.Therefore, there are also the application examples of nitric oxide donors in the prior art, but it is in application, usually will be with
Exciting agent uses simultaneously, is just able to achieve the decomposition of nitric oxide donors, nitric oxide donors point under room temperature and general damp condition
Solution is very slow seldom, it is difficult to reach requirement.But inventor is the study found that under high humidity conditions, nitric oxide donors can
To be directly sustained nitric oxide, and the humidity of the gas of characteristics of contaminated respiratory droplets can satisfy this condition just, is based on this, forms this hair
Bright technology, i.e., it is of the invention can be from providing nitric oxide production mouth and nose amenities, without adding other substances or exciting agent
In the case of nitric oxide production sustained release can be achieved, during use, nitric oxide donors sustained release nitric oxide gas can reach 4 ×
10-10mol·cm-2·min-1Above concentration, and can be sustained release 5 hours or more, to microorganisms such as bacteriums in air and exhale
The bacterium carried in outlet all has wide spectrum suppression and kills effect.
In terms of the area of basis material, when nitric oxide donors select s-nitroso-N-acetylpenicillamine, load capacity
Or the dosage being encapsulated in inside basis material is preferably equal to or greater than 6.3mg/cm2；When nitric oxide donors select S-nitrosoglutathione paddy
When the sweet peptide of Guang, load capacity or the dosage being encapsulated in inside basis material are preferably equal to or greater than 10.6mg/cm2.In this dosage
Under, mouth and nose amenities of the invention during use, not only can guarantee that nitric oxide donors continue 5 hours or more to be sustained an oxygen
Change nitrogen and reaches 4 × 10-10mol·cm-2·min-1Above concentration, simultaneously, it may have excellent fungistatic effect.
Compared with the first technical solution, it is added to reducing agent in second of technical solution, which can be further
It excites nitric oxide donors to decompose and generates NO, accelerate the rate that nitric oxide donors decompose, facilitate NO in mouth and nose amenities
Quick release.
Embodiment 1
Using flannelette as base material, area load nitric oxide donors simultaneously study its nitric oxide (NO) release conditions.
Select flannelette as base material, s-nitrosoglutathione (GSNO) is used as NO donor, and NO donor passes through physics
Mode is carried on flannelette substrate surface.Specifically, 764mg NO donor powder is dispersed in 3mL aqueous solution, by the solution
It is dispersed in the flannelette surface having a size of 6cm × 6cm, is quickly dried up by cold wind, is obtained to surface and be attached with the suede of NO donor
Cloth.
There are the flannelette of NO donor, simulated respiration condition based on the above-mentioned load being prepared, research support materials discharge NO
Substantially situation.Specifically, being crossed aqueous solution (flow velocity: 0.7L/min) by air pump blow air, gas enters equipped with load
There is the closed container of NO donor material, is detected by nitrogen-oxide analyzer, (NOA).
NO donor flannelette is loaded under the conditions of simulated respiration, NO release conditions such as Fig. 1.
By Figure 1A it is observed that under the conditions of simulated respiration, load NO donor material can be released constantly for a long time
NO gas, and NO2Concentration is extremely low, can almost ignore；In addition, it is converted into NO Flux, it is known that within the testing time,
Nitric oxide donors can be sustained NO for a long time, at concentrations up to 1.1 × 10-9mol·cm-2·min-1(Figure 1B) above.Existing text
Middle record is offered, is with Escherichia coli, staphylococcus aureus, staphylococcus epidermis, Klebsiella Pneumoniae and pseudomonas aeruginosa
Example, material surface maintain 4 × 10-10mol·cm-2·min-1Nitric oxide releasing amount 3 hours, it can significantly inhibition bacterium
Growth, count of bacteria value show 106The reduction of power times；As it can be seen that the material of load NO donor of the invention is for mouth and nose health
In apparatus, under normal breathing conditions, it can be realized NO from providing, effectively realize good bacteriostatic activity.
The antibacterial activity of 2 area load nitric oxide donors material of experimental example
Select GSNO to be used as NO donor, flannelette as base material, be prepared load different NO for the scale of construction (382mg,
573mg, 764mg) flannelette material (specific the preparation method is the same as that of Example 1), such as Fig. 2, with increasing for GSNO load capacity, sample
Surface color is deepened.The round flannelette material that diameter is 6mm is prepared by punch.
The LB solid culture that 15~20mL melts is added to be based in sterilizes culture dish, after solidification to be cooled, takes 0.2mL concentration
About 1 × 108The suspension bacteria liquid of CFU/mL utilizes aseptic cotton carrier even spread.After 2~3 minutes, it will be attached with not by tweezers
It is gently affixed on culture dish surface with the flannelette disk for the scale of construction, selects the flannelette without any processing as a control group.It will finally attach
There is the culture dish of sample to be placed in 37 DEG C of climatic chambers and is inverted culture.The inhibition zone that each flannelette original on piece occurs after 16 hours is big
It is small such as Fig. 3, wherein 1 is represented as flannelette of the surface without any processing, 2,3,4 respectively represent area load have 382mg, 573mg,
The flannelette disk of 764mg NO donor measures the antibacterial circle diameter occurred on each flannelette disk, and final result is averaged
Value ± standard deviation (n=3), test result is as follows table 1.
The antibacterial circle diameter size of 1 area load of the table not flannelette material of same amount NO donor
By Fig. 3 it is observed that area load has the flannelette of NO donor to have significant fungistatic effect, and combine table 1
Data, it is known that antibacterial circle diameter has NO for scale of construction dependence, with the increase for the scale of construction, antibacterial circle diameter becomes larger, when
Nitric oxide donors attachment density is 21.2mg/cm on flannelette surface2When, antibacterial circle diameter is up to 16.8mm, shows excellent
Antibacterial effect.
The fusion spray cloth that experimental example 3 is attached with different bacteriostatic agents tests the inhibition of Escherichia coli
The round fusion spray cloth that diameter is 4.5mm is prepared by punch, prepares s-nitroso-N-acetylpenicillamine
(SNAP) concentration is the THF solution of 100mg/mL, and the donor solution of different volumes is added dropwise on muti-piece fusion spray cloth respectively, and THF is kept away
Photoablation, so that fusion spray cloth surface is attached with 0.5mg, 1.0mg, 1.5mg and 2.0mg donor respectively.15~20mL is added to melt
LB solid culture be based on sterilizes culture dish in, after solidification to be cooled, taking 0.2mL concentration is about 1 × 108The suspended bacteria of CFU/mL
Liquid utilizes aseptic cotton carrier even spread.After 2~3 minutes, the different fusion spray cloth disks for the scale of construction will be attached with by tweezers and are gently pasted
In culture dish surface, wherein selecting the fusion spray cloth without any processing, DC99 copper ion fusion spray cloth and silver ion cloth as a control group.
Finally, culture dish to be inverted to culture in 37 DEG C of climatic chambers.After 16 hours, the inhibition zone size occurred on each fusion spray cloth is such as
Fig. 4, wherein C is represented as fusion spray cloth of the surface without any processing, and 1 represents DC99 copper ion fusion spray cloth, and 2 represent silver ion cloth, and 3
~6 respectively represent the fusion spray cloth for being attached with 0.5mg, 1.0mg, 1.5mg and 2.0mg NO donor, by vernier caliper to each melt-blown
The antibacterial circle diameter occurred on cloth disk measures, and final result is averaged ± standard deviation (n=3), and test result is such as
The following table 2.
2 surface of table is attached with the antibacterial circle diameter (mm) of the fusion spray cloth of different bacteriostatic agents
By Fig. 4 it is observed that surface is attached with the fusion spray cloth sequin of donor in the culture dish for being coated with Escherichia coli
In have significant inhibition zone, and combine 2 data of table, it is known that antibacterial circle diameter have for the scale of construction dependence, with
Surface attachment increases for the scale of construction, and antibacterial circle diameter becomes larger.It is worth noting that, when 1mg donor is adhered on fusion spray cloth surface,
Antibacterial circle diameter is up to 12.4mm, and being higher than silver ion cloth and DC99 copper ion fusion spray cloth, (antibacterial circle diameter is respectively 9.9 Hes
0mm)。
The antibacterial activity of 4 nitric oxide donors interlayer of embodiment
Select GSNO as nitric oxide donors, wherein substrate is flannelette.Specifically, weigh respectively quality be 3.0mg,
The GSNO solid powder of 4.5mg, 6.0mg, solid powder are laid in the circle flannelette surface that diameter is 6mm, and upper layer is stamped same big
The sandwich for including nitric oxide donors is prepared in small flannelette.The LB solid culture that 15~20mL melts is added to be based on
In sterilizes culture dish, after solidification to be cooled, taking 0.2mL concentration is about 1 × 108The suspension bacteria liquid of CFU/mL, utilizes aseptic cotton carrier
Even spread.The interlayer disk containing different nitric oxide donors amounts is gently affixed on culture dish surface by tweezers, in 37 DEG C of perseverances
Culture is inverted in constant temperature and humidity case.After 16 hours, observes and measure antibacterial circle diameter size in culture dish, such as Fig. 5, wherein 1,2,3
Respectively represent the flannelette disk that area load has 3.0mg, 4.5mg, 6.0mg GSNO.Measure occur on each flannelette disk it is antibacterial
Loop diameter, final result are expressed as average value ± standard deviation (n=3), test result is as follows table 3.
The antibacterial activity of 3 nitric oxide donors interlayer of table
As shown in Figure 5, different amounts of NO donor is adhered in interlayer and shows different bacteriostatic activities, there is concentration dependant
Property, as NO increases for the scale of construction, antibacterial situation is more obvious.When in interlayer NO for the scale of construction be 6.0mg when, antibacterial circle diameter can reach
12.5mm。
5 solid bacteriostatic agent of embodiment NO release conditions under the effect of different reducing agents
Weigh respectively GSNO (0.012mmol) and reducing agent (0.012mmol) (glutathione (GSH)/citric acid (CA)/
Ascorbic acid (VC)), it is uniformly mixed, sheet bacteriostatic agent is made by infrared tablet press machine.Human body respiration condition is simulated, it is solid to test this
The nitric oxide releasing situation of body bacteriostatic agent.The air that flow velocity is 0.7L/min, through solid bacteriostatic agent, is examined by steam by NOA
NO donor rate of release is surveyed, wherein nitric oxide donors are as experiment contrast.
Human body respiration condition is simulated, solid bacteriostatic agent has different rates of release, such as scheme under the effect of different reducing agents
5.Reducing agent can be used as the exciting agent of nitric oxide donors, can accelerate nitric oxide donors and decompose the rate for generating NO.Addition
After different reducing substances, NO donor decomposition rate has different degrees of promotion in solid bacteriostatic agent.Wherein citric acid (CA) plus
Enter, the decomposition rate of NO donor only has slight growth；Solid bacteriostatic agent added with ascorbic acid (VC), greatly improves
The decomposition rate of NO donor, NO concentration reach as high as 1600ppb；After adding glutathione (GSH), NO concentration in solid bacteriostatic agent
As the time gradually increases, and NO is able to maintain that and discharges for a long time that catalytic decomposition efficiency is lower than ascorbic acid.
Claims (10)
1. one kind can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that the mouth and nose amenities includes substrate material
Expect and can provide certainly nitric oxide production solid bacteriostatic agent, which is nitric oxide donors or nitric oxide donors
With the mixture of reducing agent；The solid bacteriostatic agent is supported on substrate material surface, or is encapsulated in inside base material.
2. according to claim 1 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that an oxidation
Nitrogen donor is s-nitroso-N-acetylpenicillamine, s-nitrosoglutathione, azo alkene father-in-law's glycol series compound, azo alkene
One or more of father-in-law's glycol carried polymer.
3. according to claim 1 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that the substrate material
Material is fiber felt pan, high-molecular fabric, flannelette, fusion spray cloth or cellulose membrane.
4. according to claim 1 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that the reducing agent
For one or more of glutathione, citric acid, ascorbic acid, cysteine.
5. according to claim 2 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that an oxidation
Nitrogen donor is s-nitroso-N-acetylpenicillamine, and dosage is more than or equal to 6.3mg/cm2。
6. according to claim 2 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that an oxidation
Nitrogen donor is s-nitrosoglutathione, and dosage is more than or equal to 10.6mg/cm2。
7. according to claim 1 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that the solid suppression
Microbial inoculum be nitric oxide donors and reducing agent mixture, wherein the molar ratio of nitric oxide donors and reducing agent be 1:0.1~
10。
8. according to claim 1 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that the solid suppression
Microbial inoculum is nitric oxide donors, and the nitric oxide donors are loaded to the method on basis material are as follows: by nitric oxide donors
It is dissolved in organic solvent or water, nitric oxide donors solution is made, then pass through solution swelling method, spraying, drop coating or dipping
Method loads nitric oxide donors in substrate material surface；Or nitric oxide donors are fixed on base material by chemical modification
On.
9. according to claim 1 can be from the nitric oxide production mouth and nose amenities of offer, which is characterized in that the solid suppression
Microbial inoculum is the mixture of nitric oxide donors and reducing agent, is loaded to the method on basis material are as follows: by solid bacteriostatic agent
It is dissolved by organic solvent, substrate material surface is then supported on by solution swelling method, drop coating, infusion process, obtaining load has
The base material of solid bacteriostatic agent.
10. according to claim 8 or claim 9 can be from providing nitric oxide production mouth and nose amenities, which is characterized in that it is described to have
Solvent is tetrahydrofuran or alcohols.
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| CN111838841A (en) * | 2020-08-06 | 2020-10-30 | 南京诺全生物医疗科技有限公司 | Antibacterial functional material and preparation method and application thereof |
| CN111910441A (en) * | 2020-08-06 | 2020-11-10 | 南京诺全生物医疗科技有限公司 | A kind of antibacterial chip and its preparation method and application |
| CN111962307A (en) * | 2020-08-06 | 2020-11-20 | 南京诺全生物医疗科技有限公司 | Antibacterial material capable of providing nitric oxide by itself and preparation method and application thereof |
| CN114667166A (en) * | 2019-08-23 | 2022-06-24 | 诺塔实验室有限责任公司 | Nitric Oxide Generation System |
| CN115944584A (en) * | 2023-01-18 | 2023-04-11 | 南京诺令生物科技有限公司 | Gel product for gynecology and preparation method and application thereof |
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Application publication date: 20190607 |