CN109845876A - Highly purified soybean oligopeptide and preparation method and application with unique solubility for high energy - Google Patents
Highly purified soybean oligopeptide and preparation method and application with unique solubility for high energy Download PDFInfo
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Abstract
The present invention disclose it is a kind of with unique solubility for high can highly purified soybean oligopeptide and preparation method and application.The present invention is digested, enzyme deactivation, activated carbon adsorption decoloration, separation of solid and liquid, obtained liquid ultrafiltration, nanofiltration, concentration is sterilized, and drying obtains the highly purified soybean oligopeptide with unique solubility for high energy by the way that soybean separation protein white powder is dispersed aquation.Oligopeptide protein content >=94%, peptide content >=91%, the accounting that relative molecular weight is less than 1000Da peptide is 83.7%~85.6%, and the accounting of free amino acid is 0.32%~0.41%, and sodium content is 48.7~56.6mg/100g；Not conglomeration, Gly-His-Lys are presented in course of dissolution directly quickly to dissolve such as snowslide on aqueous solution surface, without the extremely strong sensory experience such as obvious bubble, and it is mouthfeel free from extraneous odour, bitter taste, soft, the quality raw materials that can be used as health food, special doctor's food, ordinary food, are suitable for various people.
Description
Technical field
The present invention relates to a kind of intensive processing techniques of soybean protein, and in particular to a kind of height with unique solubility for high energy
Purity soybean oligopeptide and preparation method and application.
Background technique
Soybean peptide is product of the soybean protein through enzymatic isolation method or Production by Microorganism Fermentation.Its compared with soybean protein,
It is absorbed with easy, human body energy can be supplemented rapidly, sensitization is low, the heat-resisting, spies such as acid resistance is strong, and solution viscosity is low, emulsibility is good
Property, in addition with more strengthen immunity, antifatigue, anti-oxidant, lowering blood pressure and blood fat, norcholesterol, promotion fat metabolism, rush
Into the unique physiological function such as mineral absorption.Therefore, soybean peptide has become the deep processing that soybean protein is novel, most potential
Product has very wide prospect in functional food (especially health food, sports food), medicine and other fields.
Commercially available soybean peptide type of merchandize is various, widely used.The technique for preparing human consumption soybean peptide at present mainly digests
Method, albumen after the heavy purification of dregs of beans (protein content >=50%) alkali soluble acid or directly by soybean protein isolate (in terms of butt,
Protein content >=90%) be raw material, then add edible protein enzyme controlled enzymatic hydrolysis, separation and purification, concentrate drying are made, product is adopted
With standard GB/T/T 22492-2008 " soy peptide powder ", including grade III Standard.But either using dregs of beans as raw material, still
There are the following problems for the soybean peptide produced using protein isolate as raw material: (1) lipidated protein is low, reaches the regulation of GB/T 22492
Primary standard soybean peptide product protein content >=90% (in terms of butt), ash content it is higher (in terms of butt, GB/T 22,492 1
Grade standard requires≤6.5%, and sodium salt content is higher, and part population such as Hypertensive Population is edible to be restricted), also containing a small amount of
Carbohydrate (1~3% differs), hygroscopicity is strong, moisture-sensitive；(2) peptide content, oligomeric peptide content are relatively low, GB/T22492 rule
Determine peptide content (in terms of butt) >=55%, relative molecular mass≤5000 of >=80% peptide fragment can be described as " peptide ", primary standard
It is required that relative molecular mass≤2000 of peptide content (in terms of butt) >=80%, >=80% peptide fragment, it is seen that soybean peptide product at present
Peptide content is relatively low, and oligopeptide (referring generally to 1000 or less relative molecular mass) content is relatively low, and product efficacy is not significant, function is not dashed forward
Out；(3) although product is completely soluble, due to soybean peptide emulsibility, foaming characteristic is preferable, hygroscopicity is strong, easy to knot groups after molten water
It bubbles through the water column and generates a large amount of blistering, dissolution time is long, sense organ is poor, limits the application of soybean peptide product.
Summary of the invention
In order to solve the difficult point and shortcoming of the above-mentioned prior art, the primary purpose of the present invention is that providing one kind has
The preparation method of the highly purified soybean oligopeptide of unique solubility for high energy.The preparation method passes through using soybean protein isolate as raw material
Disperse aquation, compound protease is added, is enzymolyzing alpha-amylase, activated carbon adsorption enzyme deactivation, centrifuge separation, film ultrafiltration, nanofiltration, dense
Contracting, drying are made.
Another object of the present invention is to provide what is obtained by above-mentioned preparation method to have the high-purity of unique solubility for high energy
Spend soybean oligopeptide, peptides proteins content >=94% (in terms of butt), peptide content >=90% (in terms of butt), in course of dissolution
Not conglomeration, Gly-His-Lys are presented directly quickly to dissolve such as snowslide on aqueous solution surface, without the extremely strong sensory experience such as obvious bubble, and
Mouthfeel free from extraneous odour, bitter taste, soft, which can be used as the raw material of health food, special doctor's food, ordinary food.
A further object of the present invention is to provide the applications of the above-mentioned highly purified soybean oligopeptide with unique solubility for high energy.
The present invention is realized by following technical proposals: a kind of high-purity soybean with unique solubility for high energy is oligomeric
The preparation method of peptide, comprising the following steps:
(1) dispersion of soybean separation protein white powder, aquation: being formulated as protein milk with deionized water for soybean separation protein white powder,
Soybean protein isolate is starched into dispersion and aquation by shearing；
(2) digest: regulatory protein starches pH value to 8~10, addition be equivalent to soybean separation protein white powder quality 0.5%~
1.5% alkali protease hydrolyzes 3h~10h in 50 DEG C~55 DEG C stirrings, adds and is equivalent to soybean separation protein white powder quality
0.2%~1% neutral proteinase and the alpha-amylase of soybean separation protein white powder quality 0.2%~1.0% is equivalent in 50 DEG C
~55 DEG C of stirrings hydrolyze 1h~2h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, heating is added active carbon powder and stirs and evenly mixs, and constant temperature stirs
It mixes, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) it is separated by solid-liquid separation: the protein milk after enzyme deactivation, activated carbon adsorption being separated by solid-liquid separation, liquid is taken；
(5) ultrafiltration, nanofiltration, concentration: the liquid that step (4) is obtained carries out ultrafiltration, and filtered solution is completed de- using nanofiltration
Salt removes free amino acid, removes small molecule carbohydrate and be concentrated, and obtains concentrate；
(6) it sterilizes, is dry: by concentrate sterilization, spray drying, controlling the Relative bulk density of finished powder, collect finished product
Powder obtains the highly purified soybean oligopeptide with unique solubility for high energy.
In step (1):
The soybean separation protein white powder must meet GB/T20371-2006 " food industry soybean protein ", it is desirable that egg
White matter content (in terms of butt) is not less than 90%.
The concentration of soyabean protein powder is mass percent 5%~15% in the soybean protein isolate slurry.
The condition of the shearing is preferably as follows: shear velocity is 2000rpm~3000rpm, and the time is 10~20min.
In step (2):
The pH value is adjusted by using sodium hydroxide solution.
The sodium hydroxide solution preferred concentration is the sodium hydroxide solution of 1mol/L~5mol/L.
The alkali protease refers to alkali protease, pig or the pancreas egg in the pancreas of ox source in microbial fermentation source
One or both of white enzyme；Preferably Novi, Denmark believes Alcalase2.4L alkali protease.
The additional amount of the alkali protease is preferably equivalent to soybean separation protein white powder quality 0.5%~1.2%.
The neutral proteinase refers to by the protease of the fermented extraction of bacillus subtilis；Preferably Novi, Denmark believes
Neutrase 0.8L。
The amylase is alpha-amylase；Preferably mesophilicα-diastase；More preferably Novi, Denmark believes medium temperature 480L
Type alpha-amylase.
The mixing speed of the stirring enzymatic hydrolysis is 20~50rpm；Preferably 36rpm.
In step (3):
The temperature of the heating rises to 80 DEG C~95 DEG C.
The active carbon is the active carbon that specification is 200 mesh or more.
The additional amount of the active carbon powder is preferably equivalent to the 5%~15% of soyabean protein powder quality.
The constant temperature is the maintained temperature of temperature that heating reaches.
The time of the constant temperature stirring is preferably 30~90min.
In step (4):
The separation of solid and liquid is realized preferably by centrifugation；It is more preferably centrifuged and is realized by horizontal centrifuge.
The condition of the centrifugation is preferably 3000rpm~6000rpm.
In step (5):
The ultrafiltration membrane ultrafiltration that it is 10kDa by molecular cut off that the ultrafiltration, which refers to,.
The nanofiltration membrane nanofiltration that it is 1nm by aperture that the nanofiltration, which refers to,.
The concentration refers to that being concentrated into solid content is 15%~35%.
In step (6):
The sterilization mode is preferably ultrahigh-temperature instant sterilizing (UHT).
The drying is preferably dry by spray drying tower.
The spray drying tower is preferably Centrafugal spray drying tower；More preferably LPG-250 type high speed centrifugation is spraying
Drying tower.
The operating parameter of the spray drying tower is preferably that inlet air temperature is 170 DEG C~190 DEG C, and leaving air temp is 70 DEG C
~90 DEG C, feed screw pump discharge is 8~13Hz, atomization frequency is 35~45Hz, and intake is 12000~18000m3/h.Its
In, intake should be adjusted with the size of spray drying tower, control Relative bulk density, realize production-scale amplification and contracting
It is small.
The Relative bulk density is preferably 0.17~0.27g/mL.
A kind of highly purified soybean oligopeptide with unique solubility for high energy, is prepared by the above method.
The high-purity is protein content (in terms of butt) >=94%, peptide content (in terms of butt) >=91%；More
Body is as follows: protein content (in terms of butt) >=94%, peptide content (in terms of butt) >=91%, and relative molecular weight is less than 1000Da
The mass percent of peptide is 83.7%~85.6%, and the mass percent of free amino acid is 0.32%~0.41%, sodium content
For 48.7~56.6mg/100g, not conglomeration, Gly-His-Lys are presented in the soybean oligopeptide course of dissolution directly on aqueous solution surface as avenged
As collapsing quickly dissolution, without the extremely strong sensory experience such as obvious bubble, and it is mouthfeel free from extraneous odour, bitter taste, soft, can be used as health care food
Product, the special quality raw materials for curing food, ordinary food, are suitable for various people.
It is described with unique solubility for high can highly purified soybean oligopeptide preparing health food, special doctor's food and common
Application in food.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) soy oligosaccharides peptide product provided by the invention, the far super GB/T22492-2008 primary standard requirement of product index,
It is substantially better than market similar product, the needs of various people can be met.
(2) present invention is by the screening of enzyme, science selection protease, alpha-amylase combination gradient enzymatic hydrolysis in enzymolysis process,
Using initial slurry pH value is adjusted to 8~10, first alkali protease enzymatic hydrolysis adds neutral protein close to 6.0 or so to pH value
On the one hand enzyme, amylase enzymolysis, combination gradient enzymatic hydrolysis can give full play to the enzymatic hydrolysis feature of single enzyme, on the other hand can play combination association
With the Action advantage of synergy, enzymolysis efficiency and product yield, enzyme dosage 1~4% are improved, and avoids introducing excessive acid
Liquid, lye, production cost substantially reduce；It is mainly the starchy carbohydrate compound in protein degradation slurry that alpha-amylase, which is added, according to
The Optimum temperature and pH of alpha-amylase, selection 1~2h before enzymatic hydrolysis terminates are added, and on the one hand ensure that amylase is efficiently made
With another aspect reduces color, flavor effect caused by enzymolysis product Maillard reaction as far as possible.
(3) it is low to assign soybean by the optimal control of enzymatic hydrolysis parameter, integrated purification system, spray drying parameters by the present invention
Not conglomeration, Gly-His-Lys are presented in poly- peptide product course of dissolution directly quickly to dissolve such as snowslide on aqueous solution surface, without obvious bubble
Etc. extremely strong sensory experience, and it is mouthfeel free from extraneous odour, bitter taste, soft.
(4) present invention is by scientific optimization technique, entire to produce that preparation process is compact, efficient energy-saving and emission-reducing, including gradient
Combination enzymatic hydrolysis, enzyme deactivation and activated carbon adsorption are decolourized, and a step is completed, centrifugal ultrafiltration nanofiltration integrates refining spearation and (ensure that each fraction
From high efficiency while, and realize concentration) efficiently preparation high-quality soy oligosaccharides peptide product, process operability is strong, non-
Often it is easy to industrialize.
The high added value comprehensive utilization of soybean intensive processing by-product can be achieved in the present invention, promotes soybean intensive processing enterprise
Economic benefit, promote soybean planting, processing, cultivation benign cycle.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Embodiment 1
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add Neutrase 0.8L (the Novi's letter biology for being equivalent to albumen powder quality 0.6%
Technology Co., Ltd.'s commercial enzyme), be equivalent to albumen powder quality 0.6% medium temperature 480L type alpha-amylase (Novi believe biotechnology
Co., Ltd's commercial enzyme), 1.5h is hydrolyzed in 53 DEG C of stirrings；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, and obtain high-purity has unique solubility for high energy
Soy oligosaccharides Gly-His-Lys.
Embodiment 2
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 5%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2000rpm with blender, aquation 20min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 1mol/L and starch pH value to 8.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 0.5% (is stirred in 50 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 10h, add Neutrase 0.8L (the biological skill of Novi's letter for being equivalent to albumen powder quality 0.2%
Art Co., Ltd commercial enzyme), be equivalent to albumen powder quality 0.2% medium temperature 480L type alpha-amylase (Novi letter biotechnology have
Limit company commercial enzyme) in 50 DEG C of stirrings hydrolysis 2h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 80 DEG C are warming up to, is added relative to soyabean protein powder quality
5% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 30min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 3000rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 15% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 170 DEG C of inlet air temperature, 70 DEG C of leaving air temp, feed screw pump discharge 8Hz, atomization frequency 35Hz, intake 12000m3/
H, the Relative bulk density for controlling finished powder is 0.17g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Embodiment 3
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 15%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 3000rpm with blender, aquation 10min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 5mol/L and starch pH value to 10.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 55 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 3h, add Neutrase 0.8L (the biological skill of Novi's letter for being equivalent to albumen powder quality 1.0%
Art Co., Ltd commercial enzyme), 1.0% medium temperature 480L type alpha-amylase (Novi believes Bioisystech Co., Ltd's commercial enzyme) is in 55
DEG C stirring hydrolysis 1h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 95 DEG C are warming up to, is added relative to soyabean protein powder quality
15% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 90min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 6000rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 35% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 190 DEG C of inlet air temperature, 90 DEG C of leaving air temp, feed screw pump discharge 13Hz, atomization frequency 45Hz, intake 18000m3/
H, the Relative bulk density for controlling finished powder is 0.27g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Embodiment 4
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2000rpm with blender, aquation 20min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 2mol/L and starch pH value to 8.5, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.2% (is stirred in 52 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 5h, add Neutrase 0.8L (the biological skill of Novi's letter for being equivalent to albumen powder quality 0.5%
Art Co., Ltd commercial enzyme), 0.5% medium temperature 480L type alpha-amylase (Novi believes Bioisystech Co., Ltd's commercial enzyme) is in 52
DEG C stirring hydrolysis 1.5h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 92 DEG C are warming up to, is added relative to soyabean protein powder quality
12% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4000rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 185 DEG C of inlet air temperature, 85 DEG C of leaving air temp, feed screw pump discharge 11Hz, atomization frequency 40Hz, intake 17000m3/
H, the Relative bulk density for controlling finished powder is 0.25g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Comparative example 1
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add medium temperature 480L type alpha-amylase (the Novi's letter for being equivalent to albumen powder quality 0.6%
Bioisystech Co., Ltd's commercial enzyme) in 53 DEG C of stirrings hydrolysis 1.5h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Comparative example 2
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add Neutrase 0.8L (the Novi's letter biology for being equivalent to albumen powder quality 0.6%
Technology Co., Ltd.'s commercial enzyme) in 53 DEG C of stirrings hydrolysis 1.5h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Comparative example 3
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add Neutrase 0.8L (the Novi's letter biology for being equivalent to albumen powder quality 0.6%
Technology Co., Ltd.'s commercial enzyme), 0.6% medium temperature 480L type alpha-amylase (Novi believes Bioisystech Co., Ltd's commercial enzyme) in
53 DEG C of stirrings hydrolyze 1.5h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
4% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) supernatant after centrifuge separation ultrafiltration, nanofiltration, concentration: is passed through into molecular cut off 10kDa ultrafiltration, filtered solution
The nanofiltration membrane nanofiltration for being 1nm using aperture completes desalination, removes free amino acid, removes small molecule carbohydrate and be concentrated into solid content
Content is 25%；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Comparative example 4
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add Neutrase 0.8L (the Novi's letter biology for being equivalent to albumen powder quality 0.6%
Technology Co., Ltd.'s commercial enzyme), 0.6% medium temperature 480L type alpha-amylase (Novi believes Bioisystech Co., Ltd's commercial enzyme) in
53 DEG C of stirrings hydrolyze 1.5h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 11000m3/
H, the Relative bulk density for controlling finished powder is 0.16g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Comparative example 5
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L. and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add Neutrase 0.8L (the Novi's letter biology for being equivalent to albumen powder quality 0.6%
Technology Co., Ltd.'s commercial enzyme), 0.6% medium temperature 480L type alpha-amylase (Novi believes Bioisystech Co., Ltd's commercial enzyme) in
53 DEG C of stirrings hydrolyze 1.5h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 19000m3/
H, the Relative bulk density for controlling finished powder is 0.28g/mL, collects finished powder, obtains the soybean of high-purity unique solubility for high energy
Oligomeric Gly-His-Lys.
Comparative example 6
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add papain (the Nanning Pang Bo biology work for being equivalent to albumen powder quality 0.6%
Company, journey Co., Ltd commercial enzyme), being equivalent to the medium temperature 480L type alpha-amylase of albumen powder quality 0.6%, (Novi believes biological skill
Art Co., Ltd commercial enzyme), 1.5h is hydrolyzed in 53 DEG C of stirrings；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, and obtain high-purity has unique solubility for high energy
Soy oligosaccharides Gly-His-Lys.
Comparative example 7
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When the Alcalase2.4L (Novi believes Bioisystech Co., Ltd's commercial enzyme) in albumen powder quality 1.0% (is stirred in 53 DEG C of stirrings
Mixing speed is 36rpm) hydrolysis 6.5h, add Neutrase 0.8L (the Novi's letter biology for being equivalent to albumen powder quality 0.6%
Technology Co., Ltd.'s commercial enzyme), be equivalent to albumen powder quality 0.2% Flavourzyme 1000L (Novi letter biotechnology have
Limit company commercial enzyme), 1.5h is hydrolyzed in 53 DEG C of stirrings；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, and obtain high-purity has unique solubility for high energy
Soy oligosaccharides Gly-His-Lys.
Comparative example 8
(1) soybean separation protein white powder the dispersion of soybean separation protein white powder, aquation: is configured to quality percentage with deionized water
Than the protein milk for 10%, soyabean protein powder shearing is uniformly dispersed with the revolving speed of 2500rpm with blender, aquation 15min；
(2) it digests: using concentration to adjust regulatory protein for the sodium hydroxide solution of 3mol/L and starch pH value to 9.0, phase is added
When in albumen powder quality 1.0% Alcalase2.4L (Novi believe Bioisystech Co., Ltd's commercial enzyme), be equivalent to albumen powder
The Neutrase 0.8L (Novi believes Bioisystech Co., Ltd's commercial enzyme) of quality 0.6%, it is equivalent to albumen powder quality 0.6%
Medium temperature 480L type alpha-amylase (Novi believe Bioisystech Co., Ltd's commercial enzyme), in 53 DEG C of stirrings, (mixing speed is
36rpm) hydrolyze 8h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, 90 DEG C are warming up to, is added relative to soyabean protein powder quality
10% active carbon powder (more than 200 mesh) stirs and evenly mixs, and constant temperature stirs 60min, realizes enzyme deactivation, activated carbon adsorption decoloration；
(4) be centrifugated: by the protein milk after enzyme deactivation, activated carbon adsorption be pumped into horizontal spiral centrifuge with 4500rpm from
The heart removes slag, and takes supernatant；
(5) ultrafiltration, nanofiltration, concentration: the supernatant after centrifuge separation is surpassed by the ultrafiltration membrane that molecular cut off is 10kDa
Filter, the nanofiltration membrane nanofiltration that filtered solution is 1nm using aperture complete desalination, remove free amino acid, remove small molecule carbohydrate and be concentrated
It is 25% to solid content；
(6) sterilize, is dry: concentrate is pumped into the drying of LPG-250 type hig h-speed centrifugal spray drying tower, choosing after UHT is sterilized
Select 180 DEG C of inlet air temperature, 80 DEG C of leaving air temp, feed screw pump discharge 10Hz, atomization frequency 40Hz, intake 15000m3/
H, the Relative bulk density for controlling finished powder is 0.22g/mL, collects finished powder, and obtain high-purity has unique solubility for high energy
Soy oligosaccharides Gly-His-Lys.
Soybean peptide made from Examples 1 to 4 and comparative example 1~8 is measured using method as defined in GB/T 22492-2008
Powder gross protein value, peptide content, gross protein middle-molecular-weihydroxyethyl≤1000Dal peptide content, content of ashes, moisture content, thick rouge
Fat content and urease activity；It is detected using GB 5009.91-2017 method big made from Examples 1 to 4 and comparative example 1~5
Beans Gly-His-Lys sodium content；" the edible analyzing food nutrition components of mirowave handbook " edited using Yang Yuexin, Wang Guangya (publish by light industry of China
Society, the first edition in 2002) it is big made from the free amino acid analysis method detection Examples 1 to 4 and comparative example 1~8 that provide
Free amino acid quality in beans Gly-His-Lys；Soy peptide powder made from Examples 1 to 4 and comparative example 1~8 is analyzed using following method
Dissolved state: 500mL beaker is used, 43 DEG C of drinking pure waters of 300mL is taken uniformly to be added to after taking 5g sample to be installed with hermetic bag
On the water surface, timing；Without stirring, the dissolved state of product is observed；After product is completely dissolved, stop timing, note
Dissolution time is recorded, solution state is observed；Above each Indexs measure equal parallel laboratory test 3 times, results are averaged, and concrete outcome is shown in Table
1, table 2, table 3.
Soy peptide powder analysis detection result made from 1 Examples 1 to 4 of table
Soy peptide powder analysis detection result made from 2 comparative example 1~5 of table
Soy peptide powder analysis detection result made from 3 comparative example 6~8 of table
As shown in Table 1, soy peptide powder protein content (in terms of butt) made from Examples 1 to 4 be 94.6%~
95.7%, peptide content (in terms of butt) is 91.4~92.8%, and gross protein middle-molecular-weihydroxyethyl≤1000Dal peptide content is 83.7%
~85.6%, content of ashes (in terms of butt) is 1.22%~1.35%, and free aminoacid content is 0.32%~0.41%, sodium
Content is 48.7~56.6mg/100g, and each index is much better than the similar production of primary standard requirement and market in GB/T22492-2008
Product, and the product is added in 43 DEG C of warm water, under not stirring condition, powder floats on solution surface and quickly dissolves, no conglomeration, without gas
Bubble does not lose powder, and 160s~182s dissolution finishes, clear after dissolution；It can be seen that being tool using the product that the present invention is prepared
There is the high-quality soybean oligopeptide of unique solubility property.
Compared with embodiment 1, during comparative example 1 is not added with neutral proteinase (Neutrase 0.8L), comparative example 2 is not added with
Warm 480L type alpha-amylase, comparative example 3 add amounts of activated carbon relatively low (not up to soyabean protein powder quality 5%~15%), comparison
In example 4 and comparative example 5 control finished powder Relative bulk density not between 0.17~0.27, comparative example 6 use papain
Substitution neutral proteinase, comparative example 7 are not added with medium temperature 480L type alpha-amylase but are added to flavor protease Flavourzyme
The synchronous addition Alcalase2.4L, Neutrase 0.8L, medium temperature 480L type alpha-amylase of 1000L, comparative example 8.By 2 result of table
It is found that using single alkali protease, under protein content of product, peptide content, molecular weight≤1000Dal peptide content are obvious
Drop, and dissolution time extends, has slight picking；Do not add alpha-amylase, protein content of product, peptide content, molecular weight≤
1000Dal peptide content is decreased obviously, and has slight picking in course of dissolution；Amounts of activated carbon additive amount is insufficient, protein content of product,
Peptide content also has certain decline, and dissolution time is obviously partially long, has that a small amount of conglomeration, bubble be more, slight picking；Finished powder it is opposite
Bulk density is relatively low, and component target influence is unobvious, but has a small amount of conglomeration, slight picking in course of dissolution；Finished powder it is opposite
Bulk density is higher, and component target influences unobvious, but bubble is more in course of dissolution, obvious picking, and solution rate is fast.By table
3 it is found that after using Papain enzymes extraction neutral proteinase, protein content of product, peptide content, molecular weight≤1000Dal peptide
Content is decreased obviously, and blister in course of dissolution it is more, have slight picking；Add flavor protease Flavourzyme
After 1000L, protein content of product difference is unobvious, but peptide content, molecular weight≤1000Dal peptide content are decreased obviously, trip
Content in bleeding sap from stem increase, while blister in course of dissolution it is more, have slight picking；Synchronous addition Alcalase2.4L,
Neutrase 0.8L, medium temperature 480L type alpha-amylase, one side protein content, peptide content, molecular weight≤1000Dal peptide contain
Amount decline, dissolution time extend, have a small amount of bubble, slight picking in course of dissolution, on the other hand due in product oligopeptide with
Carbohydrate Mei Lade long action time, finished product are still partially light yellow through decoloration color.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of preparation method of the highly purified soybean oligopeptide with unique solubility for high energy, it is characterised in that including following step
It is rapid:
(1) dispersion of soybean separation protein white powder, aquation: soybean separation protein white powder is formulated as protein milk with deionized water, is passed through
Soybean protein isolate is starched dispersion and aquation by shearing；
(2) digest: regulatory protein starches pH value to 8~10, and addition is equivalent to soybean separation protein white powder quality 0.5%~1.5%
Alkali protease hydrolyzes 3h~10h in 50 DEG C~55 DEG C stirrings, add be equivalent to soybean separation protein white powder quality 0.2%~
1% neutral proteinase and the alpha-amylase for being equivalent to soybean separation protein white powder quality 0.2%~1.0% are stirred in 50 DEG C~55 DEG C
Mix hydrolysis 1h~2h；
(3) enzyme deactivation, activated carbon adsorption decoloration: after enzymatic hydrolysis, heating is added active carbon powder and stirs and evenly mixs, constant temperature stirring；
(4) it is separated by solid-liquid separation: the protein milk after enzyme deactivation, activated carbon adsorption being separated by solid-liquid separation, liquid is taken；
(5) ultrafiltration, nanofiltration, concentration: the liquid that step (4) is obtained carries out ultrafiltration, and filtered solution is concentrated using nanofiltration
Liquid；
(6) it sterilizes, is dry: by concentrate sterilization, spray drying, controlling the Relative bulk density of finished powder, collect finished powder, obtain
There must be the highly purified soybean oligopeptide of unique solubility for high energy.
2. the preparation method of the highly purified soybean oligopeptide according to claim 1 with unique solubility for high energy, feature
It is:
The additional amount of active carbon powder described in step (3) is equivalent to the 5%~15% of soyabean protein powder quality；
Relative bulk density described in step (6) is 0.17~0.27g/mL.
3. the preparation method of the highly purified soybean oligopeptide according to claim 1 or 2 with unique solubility for high energy, special
Sign is:
Alkali protease described in step (2) is alkali protease, pig or the pancreas of the ox source in microbial fermentation source
One or both of trypsase；
Neutral proteinase described in step (2) is the protease by the fermented extraction of bacillus subtilis；
Amylase described in step (2) is alpha-amylase.
4. the preparation method of the highly purified soybean oligopeptide according to claim 1 or 2 with unique solubility for high energy, special
Sign is:
The condition of shearing described in step (1) is as follows: shear velocity is 2000rpm~3000rpm, and the time is 10~20min；
Ultrafiltration described in step (5) is the ultrafiltration membrane ultrafiltration for being 10kDa by molecular cut off；
Nanofiltration described in step (5) is the nanofiltration membrane nanofiltration for being 1nm by aperture；
Drying described in step (6) is dry by spray drying tower.
5. the preparation method of the highly purified soybean oligopeptide according to claim 4 with unique solubility for high energy, feature
It is:
The operating parameter of the spray drying tower is as follows: inlet air temperature is 170 DEG C~190 DEG C, and leaving air temp is 70 DEG C~90
DEG C, feed screw pump discharge is 8~13Hz, atomization frequency is 35~45Hz, and intake is 12000~18000m3/h。
6. the preparation method of the highly purified soybean oligopeptide according to claim 1 or 2 with unique solubility for high energy, special
Sign is:
The protein content of soybean separation protein white powder described in step (1) is not less than 90% in terms of butt；
The concentration of soyabean protein powder is mass percent 5%~15% in the slurry of soybean protein isolate described in step (1)；
Active carbon described in step (3) is the active carbon that specification is 200 mesh or more；
Concentration described in step (5) refers to that being concentrated into solid content is 15%~35%.
7. the preparation method of the highly purified soybean oligopeptide according to claim 1 or 2 with unique solubility for high energy, special
Sign is:
PH value described in step (2) is adjusted by using sodium hydroxide solution；
The mixing speed of stirring enzymatic hydrolysis described in step (2) is 20~50rpm；
The temperature of heating described in step (3) rises to 80 DEG C~95 DEG C；
The time of the stirring of constant temperature described in step (3) is 30~90min；
Separation of solid and liquid described in step (4) is realized by centrifugation；
Sterilization mode described in step (6) is ultrahigh-temperature instant sterilizing.
8. a kind of highly purified soybean oligopeptide with unique solubility for high energy, it is characterised in that: by any one of claim 1~7
The preparation method obtains.
9. the highly purified soybean oligopeptide according to claim 8 with unique solubility for high energy, it is characterised in that: described
With unique solubility for high can highly purified soybean oligopeptide in protein content in terms of butt >=94%, peptide content in terms of butt >=
91%, the mass percent that relative molecular weight is less than 1000Da peptide is 83.7%~85.6%, the quality percentage of free amino acid
Than being 0.32%~0.41%, sodium content is 48.7~56.6mg/100g.
10. the highly purified soybean oligopeptide with unique solubility for high energy described in claim 8 or 9 is preparing health food, spy
Cure the application in food and ordinary food.
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| CN110438188A (en) * | 2019-09-06 | 2019-11-12 | 广西民族大学 | A kind of preparation method of pea protein Gly-His-Lys |
| CN111202246A (en) * | 2019-12-28 | 2020-05-29 | 江苏天美健大自然生物工程有限公司 | Soybean oligopeptide powder and preparation method thereof |
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| CN114158666B (en) * | 2021-12-10 | 2023-10-13 | 厦门元之道生物科技有限公司 | Preparation method and application of soybean oligopeptide |
| CN114214380A (en) * | 2021-12-16 | 2022-03-22 | 云南文山坤七药业股份有限公司 | Preparation method and application of soybean, coffee and American ginseng bioactive peptides |
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Application publication date: 20190607 |