CN109845500B - Method for raising seedlings of populus tomentosa by cutting tender branches - Google Patents
Method for raising seedlings of populus tomentosa by cutting tender branches Download PDFInfo
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Abstract
The invention belongs to the technical field of plant seedling raising, and particularly relates to a method for promoting the cutting seedling raising of populus tomentosa twigs. The method comprises the following steps: (1) collecting the young branches of the annual strong and semi-lignified male Chinese white poplar as cuttings, and cutting the young branches into cuttings; soaking the lower end of the cutting shoot for 0.5 to 3 hours by using a rooting agent; (2) sterilizing the cutting substrate; (3) inserting the cutting slips obtained in the step (1) into a matrix, controlling the temperature at 37-45 ℃ in the daytime and 27-30 ℃ at night, and controlling the humidity at 85-95% (4) to spray diluted carbendazim solution on the leaf surfaces for disinfection; (5) spraying a leaf fertilizer 9-10 days after cuttage; (6) hardening off seedlings 15-20 days after cuttage, shading the seedlings, and controlling the temperature and humidity in the greenhouse. The method combines the steps with each other, and greatly improves the survival rate and the propagation efficiency of the male Chinese white poplar.
Description
Technical Field
The invention belongs to the technical field of plant seedling raising, and particularly relates to a method for promoting the cutting seedling raising of populus tomentosa twigs.
Background
Populus tomentosa (Populus tomentosa Carr.) belongs to larch leaves and big trees of Populus of Salicaceae, has quick growth, straight and tall trunk, strong adaptability, developed main roots and side roots and dense branches and leaves, is one of the main tree species for urban and rural greening and four-side tree planting in North China, and is widely applied to construction of fast-growing timberland forests, shelter forests and the like. The Chinese white poplar is a male and female variant plant, the female plant is fruited and flying, and the Chinese white poplar flies all over the sky in big and medium cities such as Beijing, Tianjin, Shijiazhuang and the like in the beginning of 5 months every year, and becomes a main pollution source influencing urban environment. For this purpose, a large number of female Chinese white poplar was replaced with male Chinese white poplar.
The fine variety Lumao 50 of the populus tomentosa is an important variety popularized and applied in the current forestry production, is a male plant, and has the excellent characteristics of fast growth, straight and full dry shape, beautiful tree shape, no flying wadding and the like. The nursery stock breeding of the lumao 50 mainly depends on grafting breeding, and has the defects of labor and time consumption, low grafting survival rate, complicated seedling culture process, difficult nursery stock management and the like. However, due to the genetic characteristics and the like, the populus tomentosa belongs to the tree species with difficult rooting, the populus tomentosa is difficult to root without special treatment and cuttage, and the survival rate is low. Therefore, the method has important production practice significance for solving the problem of cutting rooting and improving the survival rate of the Chinese white poplar.
Disclosure of Invention
Aiming at the problems in the background technology, the invention provides a method for promoting the cutting seedling raising of populus tomentosa twigs. The method can promote the 50-twig Chinese white poplar root quickly and improve the survival rate of cuttage.
The invention adopts the following technical scheme:
the invention mainly provides a method for promoting rooting of cottage twig of populus tomentosa, which comprises the following steps:
(1) collecting the young branches of the annual strong and semi-lignified male Chinese white poplar as cuttings, and cutting the young branches into cuttings; soaking the lower end of the cutting with a rooting agent;
(2) sterilizing the cutting substrate;
(3) inserting the cutting slips obtained in the step (1) into a sterilized cutting medium, and spraying water thoroughly for one time to ensure that the cutting slips are fully contacted with the medium; controlling the temperature at 37-45 ℃ in the daytime, controlling the temperature at 27-30 ℃ at night, and controlling the humidity at 85-90%;
(4) disinfecting the leaf surfaces every 2-3 days; the spraying of the disinfectant can prevent the infection and the disease of the leaf surfaces;
(5) after 9-10 days of cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 2-3 days; the foliar fertilizer is sprayed to provide sufficient nutrients for rooting and growth of the cuttings;
(6) hardening seedlings after 15-20 days of cuttage, shading the rooted seedlings, gradually performing ventilation and light treatment after 3-5 days of culture, controlling the temperature at 25-30 ℃ and the humidity at 50-70%; disinfecting the leaf surfaces every 3-4 days, and spraying leaf fertilizer every 2-3 days; and (5) transplanting the seedlings after 8-12 days of cultivation.
In the rooting stage of the young branches of the Chinese white poplar, the larger the humidity is, the more favorable the rooting is, however, the invention has the unexpected discovery that when the humidity of a greenhouse is more than 90 percent, the Chinese white poplar is cultivated to root for about twenty days, although the young branches of the Chinese white poplar have rooted in a large quantity, the lower part of the cutting shoot begins to rot, and the cutting shoot is not easy to survive; according to the method, the humidity of the greenhouse is controlled to be 85-90%, so that the rooting rate of the Chinese white poplar can be improved, and meanwhile, the rotting phenomenon at the bottom of the cutting slips can be effectively prevented, and the survival rate of the transplanted Chinese white poplar is improved.
The invention finds that the effect is best when hardening seedlings after cuttage is carried out for 15-20 days in a high-temperature and high-humidity environment; less than 15 days, the root system of the seedling is not completely developed, which is not beneficial to transplanting; if the seedlings are acclimated after 20 days, the lower parts of the seedlings can be rotted, so that the seedlings are not beneficial to subsequent growth; after 3-5 days of hardening-seedling culture, gradually ventilating and exposing to light to ensure that the seedlings gradually adapt to the field environment; the temperature is controlled to be 25-30 ℃, the humidity is 50-70%, the seedlings can be promoted to adapt to the field environment more quickly, and the survival rate of seedling transplanting is improved.
Further, the twig is cut into 12-20 cm cuttings in the step (1), the rooting agent is indolebutyric acid solution, and the lower end of each cutting is soaked in the rooting agent for 0.5-3 hours. The length range of the cutting is more favorable for the shoot to root; the rooting rate of the young branches of the Chinese white poplar can be improved by adopting hormone treatment, and the rooting rate of the lufa 50 is improved most obviously by adopting an indolebutyric acid solution as a rooting agent; the method adopts the rooting agent to soak for 0.5-3 hours, so that long-time soaking is not needed, and the processing time of the cuttings is shortened.
Further, the concentration of the indolebutyric acid solution in the step (1) is 90-150 mg/L, preferably 95-110 mg/L, and more preferably 100 mg/L. Under the condition of the concentration, the rooting efficiency is optimal.
Further, half leaf is reserved for each cutting shoot in the step (1). In the experimental research process, the male Chinese white poplar, particularly the lumao 50, is found that half leaves of each cutting shoot are reserved, so that the number of the leaves and the area of the leaves are reduced, and the consumption of water and nutrients in the cutting shoot is reduced; the number of leaves carried by the cutting has influence on the rooting rate, and the excessive number of leaves can increase the consumption of nutrient components and water of the tender branches and is not beneficial to the rooting of the tender branches.
Further, the matrix in the step (2) is composed of coconut coir, perlite and vermiculite according to a mass ratio of 60-80: 15: 15; and sterilizing by using a carbendazim solution. The good substrate is the foundation for promoting the rooting and survival of the young branches of the populus tomentosa. The substrate of the invention takes coconut chaff as a main material, is matched with a small amount of perlite and vermiculite, has the characteristics of looseness, air permeability, good water retention performance and the like after being mixed according to a specific proportion, and has the substrate condition required by the rooting of the young branches of Chinese white poplar.
Further, the matrix consists of coconut coir, perlite and vermiculite according to a mass ratio of 70:15: 15. Under the condition of the mixture ratio, the effect of promoting the shoot to take root by the substrate is optimal.
Further, the daytime temperature of the step (3) is controlled to be 40-45 ℃, preferably 43 ℃.
Further, the temperature was controlled at 28 ℃ in the night in step (3).
Further, the male Chinese white poplar is lumao 50.
The high-temperature and high-humidity environment is favorable for rooting of the cutting tender branches, and for the lufa 50, the rooting is optimal when the temperature is controlled to be 40-45 ℃ in the daytime and 27-30 ℃ at night.
Furthermore, the time for raising seedlings by cutting the tender branches is from 6 to 7 in the middle of the month.
The invention has the following beneficial effects:
the invention finds that in the cutting seedling process of the lufa 50, the temperature in the daytime is controlled to be 37-45 ℃, the temperature at night is controlled to be 27-30 ℃, and the humidity of a greenhouse is 85-90%, so that the rooting of the lufa 50 tender branches is facilitated, the rooting time is shortened, and the propagation efficiency of the lufa 50 is improved.
The method combines the steps with each other, and improves the survival rate of the cottage seedling of the male Chinese white poplar twig.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 shows the rooting of lufa 50 scions after 20 days of culture in the methods for rooting by cutting in examples 1, 3 and 5 [ Steps (1) - (5) ].
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, and/or combinations thereof, unless the context clearly indicates otherwise.
In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention will be described in detail below with reference to specific embodiments.
Example 1 method for raising seedlings of populus tomentosa by cutting
The method comprises the following steps:
(1) collecting 50 robust and semi-lignified Lumao twigs growing in the current year as cuttings, cutting the tender twigs into cuttings with the length of 16cm, and reserving half leaves of each cutting; soaking the lower end of the cutting shoot for 3 hours by using 90mg/L indolebutyric acid solution;
(2) filling the cuttage substrate into a seedling raising plug tray, wherein the height of the plug tray is 15 cm; the matrix consists of crushed coconut coir, perlite and vermiculite according to a mass ratio of 60:15: 15; spraying a cutting substrate with 800-time diluted carbendazim solution to sterilize the cutting substrate, and starting cutting after 1-2 days;
(3) inserting the cutting slips obtained in the step (1) into a matrix, and spraying water for one time to ensure that the cutting slips are fully contacted with the matrix; the temperature in the daytime is controlled at 37 ℃, the temperature at night is controlled at 27 ℃, and the humidity is controlled at 85%;
(4) spraying a 800-time diluted carbendazim solution on the leaf surfaces every 2 days for disinfection;
(5) 9 days after cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 2 days;
(6) 15 days after cuttage, moving the seedling raising hole tray to another greenhouse, shading by using a shading net, gradually ventilating and exposing to light after 3 days, controlling the temperature at 30 ℃ and the humidity at 50%; spraying a carbendazim solution diluted by 1000 times on the leaf surfaces every 3 days for disinfection, and spraying a leaf fertilizer every 3 days; and culturing for 10 days to transplant the seedlings.
The seedling raising and cutting time is from the first ten days of 6 months to the middle ten days of 7 months.
Embodiment 2A method for raising seedlings of Populus tomentosa by cutting
The method comprises the following steps:
(1) collecting 50 robust and semi-lignified Lumao twigs growing in the current year as cuttings, cutting the tender twigs into 20 cm-long cuttings, and keeping half leaves of each cutting; soaking the lower end of the cutting shoot for 0.5 hour by using 150mg/L indolebutyric acid solution;
(2) filling the cuttage substrate into a seedling raising plug tray, wherein the height of the plug tray is 15 cm; the matrix consists of crushed coconut coir, perlite and vermiculite according to a mass ratio of 80:15: 15; spraying a cutting substrate with 800-time diluted carbendazim solution to sterilize the cutting substrate, and starting cutting after 1-2 days;
(3) inserting the cutting slips obtained in the step (1) into a matrix, and spraying water for one time to ensure that the cutting slips are fully contacted with the matrix; the temperature in the daytime is controlled at 45 ℃, the temperature at night is controlled at 30 ℃, and the humidity is controlled at 90%;
(4) spraying and diluting 1000 times carbendazim solution to the leaf surfaces every 3 days for disinfection;
(5) 10 days after cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 3 days;
(6) 20 days after cuttage, moving the seedling raising hole tray to another greenhouse, shading by using a shading net, gradually ventilating and exposing to light after 5 days, controlling the temperature at 25 ℃ and the humidity at 70%; spraying a carbendazim solution diluted by 1000 times on the leaf surfaces every 4 days for disinfection, and spraying a leaf fertilizer once every 3 days; and culturing for 10 days to transplant the seedlings.
The seedling raising and cutting time is from the first ten days of 6 months to the middle ten days of 7 months.
Embodiment 3A method for raising seedlings of Populus tomentosa by cutting
The method comprises the following steps:
(1) collecting 50 robust and semi-lignified Lumao twigs growing in the current year as cuttings, cutting the tender twigs into 12 cm-long cuttings, and keeping half leaves of each cutting; soaking the lower end of the cutting shoot for 3 hours by using 95mg/L indolebutyric acid solution;
(2) filling the cuttage substrate into a seedling raising plug tray, wherein the height of the plug tray is 9. cm; the matrix consists of coconut chaff, perlite and vermiculite according to the mass ratio of 70:15: 15; spraying a cutting substrate with 800-time diluted carbendazim solution to sterilize the cutting substrate, and starting cutting after 1-2 days;
(3) inserting the cutting slips obtained in the step (1) into a matrix, and spraying water for one time to ensure that the cutting slips are fully contacted with the matrix; the temperature in the daytime is controlled at 40 ℃, the temperature at night is controlled at 30 ℃, and the humidity is controlled at 90%;
(4) spraying and diluting 1000 times carbendazim solution to the leaf surfaces every 3 days for disinfection;
(5) 10 days after cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 3 days;
(6) 20 days after cuttage, moving the seedling raising hole tray to another greenhouse, shading by using a shading net, gradually ventilating and exposing to light after 3 days, controlling the temperature at 25 ℃ and the humidity at 50%; spraying a carbendazim solution diluted by 1000 times on the leaf surfaces every 4 days for disinfection, and spraying a leaf fertilizer once every 3 days; and culturing for 10 days to transplant the seedlings.
The seedling raising and cutting time is from the first ten days of 6 months to the middle ten days of 7 months.
Embodiment 4A method for raising seedlings of Populus tomentosa by cutting
The method comprises the following steps:
(1) collecting 50 robust and semi-lignified Lumao twigs growing in the current year as cuttings, cutting the tender twigs into 12 cm-long cuttings, and keeping half leaves of each cutting; soaking the lower end of the cutting shoot for 0.5 hour by using 110mg/L indolebutyric acid solution;
(2) filling the cuttage substrate into a seedling raising plug tray, wherein the height of the plug tray is 15 cm; the matrix consists of coconut chaff, perlite and vermiculite according to the mass ratio of 70:15: 15;
(3) inserting the cutting slips obtained in the step (1) into a matrix, and spraying water for one time to ensure that the cutting slips are fully contacted with the matrix; controlling the temperature at 45 ℃ in the daytime and 27 ℃ at night, and controlling the humidity in the greenhouse to be 90%;
(4) spraying a 800-time diluted carbendazim solution on the leaf surfaces every 2 days for disinfection;
(5) 10 days after cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 3 days;
(6) 20 days after cuttage, moving the seedling raising hole tray to another greenhouse, shading by using a shading net, gradually ventilating and exposing to light after 5 days, controlling the temperature at 25 ℃ and the humidity at 50%; spraying a carbendazim solution diluted by 1000 times on the leaf surfaces every 4 days for disinfection, and spraying a leaf fertilizer once every 3 days; and culturing for 10 days to transplant the seedlings.
Example 5 method for raising seedlings of Populus tomentosa by cutting
The method comprises the following steps:
(1) collecting 50 robust and semi-lignified Lumao twigs growing in the current year as cuttings, cutting the tender twigs into 20 cm-long cuttings, and keeping half leaves of each cutting; soaking the lower end of the cutting shoot for 1 hour by using 100mg/L indolebutyric acid solution;
(2) filling the cuttage substrate into a seedling raising plug tray, wherein the height of the plug tray is 15 cm; the matrix consists of coconut chaff, perlite and vermiculite according to the mass ratio of 70:15: 15; spraying a cutting substrate with 800-time diluted carbendazim solution to sterilize the cutting substrate, and starting cutting after 1-2 days;
(3) inserting the cutting slips obtained in the step (1) into a matrix, and spraying water for one time to ensure that the cutting slips are fully contacted with the matrix; controlling the temperature at 43 ℃ in the daytime and 28 ℃ at night, and controlling the humidity in the greenhouse to be 90%;
(4) spraying and diluting 1000 times carbendazim solution to the leaf surfaces every 3 days for disinfection;
(5) 9 days after cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 2 days;
(6) moving the seedling raising hole tray to another greenhouse 18 days after cuttage, shading by using a shading net, gradually ventilating and exposing to light after 4 days, controlling the temperature at 28 ℃ and the humidity at 60%; spraying a carbendazim solution diluted by 1000 times on the leaf surfaces every 4 days for disinfection, and spraying a leaf fertilizer once every 3 days; and culturing for 10 days to transplant the seedlings.
The seedling raising and cutting time is from the first ten days of 6 months to the middle ten days of 7 months.
Test examples
(1) Influence of high-temperature and high-humidity environment on cutting rooting of 50 Rumao twigs
Inserting 50 cutting slips of the luwools into a matrix, respectively controlling the temperature to be 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃ and 50 ℃, respectively having 20 cutting slips of the luwools under each temperature condition, and arranging three groups of the cutting slips in parallel; after 20 days, the survival rate and the rooting number of the single plant are counted, and the experimental results of each group are shown in the following table 1.
TABLE 1 survival rate of 50-shoot cutting and root number of single plant at different temperatures
| Temperature of | 25℃ | 30℃ | 35℃ | 40℃ | 45℃ | 50℃ |
| Survival rate/% | 30.2 | 45.6 | 50.4 | 61.3 | 56.8 | 38.5 |
| Number of roots of single plant | 4.2 | 5.1 | 5.8 | 13.4 | 8.3 | 3.8 |
As can be seen from the above table, when the temperature in the greenhouse is controlled to be 30-45 ℃, the cuttage survival rate is high, and the rooting quantity of the single plant is large.
Further, the influence of different humidity on the cutting rooting of 50 young branches of luwoo is researched
Controlling the temperature at 40 ℃, respectively controlling the humidity in the greenhouse to be 70%, 75%, 80%, 85%, 90% and 95%, wherein 50 cutting hairs are respectively arranged at 20 under each humidity condition, and three groups are arranged in parallel; after 20 days, the survival rate and the rooting number of the single plant are counted, and the experimental results of each group are shown in the following table 2.
TABLE 2 survival rate of cutting of 50 shoots of lumao under different humidity and number of roots of single plant
| Humidity | 70％ | 75％ | 80％ | 85％ | 90％ | 95％ |
| Survival rate/% | 59.4 | 64.5 | 69.6 | 71.8 | 80.4 | 68.9 |
| Number of roots of single plant | 5.2 | 6.3 | 6.9 | 7.6 | 8.5 | 8.8 |
As can be seen from Table 2, the cutting survival rate of the lufa 50 is improved in the high-temperature and high-humidity environment, and the rooting number of the single plant is increased. The invention discovers that when the humidity in a greenhouse is more than 90%, the root number of a single plant is increased, but the bottom of the cutting shoot is rotten due to a high-humidity environment, and finally the survival rate is obviously reduced.
(2) Influence of day and night temperature difference on cutting rooting of 50 Rumao twigs
The invention researches the influence of temperature difference between day and night on the rooting of the tender branches of the Lumao 50 cutting under the high-temperature and high-humidity environment condition, controls the humidity of the greenhouse to be kept at 90%, and respectively sets the temperature in the day/night as follows: 35/25 deg.C, 40/25 deg.C, 45/25 deg.C, 40/30 deg.C, 45/35 deg.C, 45/25 deg.C, respectively having 50 pieces of Lumao cutting spike, and arranging three groups in parallel; after 20 days, the survival rate is counted, and the experimental results of each group are shown in the following table 3.
TABLE 3 survival rate of 50-twig cutting of lumao at different temperatures day and night
| Day and night temperature | 35/25℃ | 40/25℃ | 45/25℃ | 40/30℃ | 45/30℃ | 45/35℃ |
| Survival rate/% | 50.3 | 70.2 | 63.6 | 88.5 | 80.4 | 70.2 |
As can be seen from table 3, when the temperature was set to 40/30 ℃ during the day/night, the survival rate of cutting with the lufa 50 was greatly increased.
(3) Effect of different hormones on rooting of cutting of 50 shoots of luwools
The invention researches the influence of different hormones on the rooting of the cutting twigs of the 50 rufa in greenhouse, controls the humidity in the greenhouse to be kept at 90 percent, sets the temperature in the day/night to be 40/30 ℃, and immerses the cutting slips in hormone solutions of cytokinin, indoleacetic acid, indolebutyric acid and naphthylacetic acid with the concentrations of 90mg/L, 120mg/L and 150mg/L respectively for 1h, wherein each group has 20 cutting slips of the 50 rufa respectively, and three groups are arranged in parallel; and after 20 days, counting the survival rate. Specific results are shown in table 4 below.
TABLE 4 survival rate of 50 twig cuttings of lumao treated with different hormones
As can be seen from Table 4 above, the survival rate of pilus 50 is significantly improved by indolebutyric acid.
(4) Influence of different matrixes on cutting rooting of 50 Rumao twigs
The invention researches the influence of different hormones on the rooting of the Rufa 50 cutting twigs, controls the humidity of a greenhouse to be kept at 90 percent, sets the temperature in the day/night to be 40/30 ℃, respectively soaks the cutting slips into indolebutyric acid solution with the concentration of 120mg/L for 1h, respectively inserts the cutting slips into the following substrates, and each group respectively has 20 cutting slips of the Rufa 50, and three groups are arranged in parallel; and after 20 days, counting the survival rate. Specific results are shown in table 5 below.
Substrate 1: perlite
Matrix 2: 1:1 ratio of perlite to vermiculite
Matrix 3: coconut husk, pearlite and vermiculite (70: 15)
Matrix 4: coconut husk
TABLE 5 survival rate of 50 twig cutting of lumao with different substrates
As shown in Table 5, the cutting survival rate of the lufa 50 is high by using the coconut coir, perlite and vermiculite as the matrix in a ratio of 70:15: 15.
(5) Different examples cutting rooting of luohao 50
On the basis of the above experiment, the parameters are further optimized to obtain the twig cutting rooting method [ steps (1) - (5) ] of the embodiments 1-5 of the invention, and the twig cutting rooting method of the embodiments 1-5 is adopted for cutting.
The control group (CK) was: collecting 50 young branches of the current-year-grown strong and semi-lignified lufa as cuttings, cutting the young branches into 12 cm-long cuttings, taking perlite as a matrix, treating the cuttings without adding hormones, controlling the culture temperature to be constant day and night, controlling the temperature to be 40 ℃ and the humidity to be 90%, spraying a carbendazim solution diluted 1000 times on the leaf surfaces every 3 days for disinfection, and spraying a leaf fertilizer every 3 days. Each group of 50 cutting shoots with rufa is 20 respectively, and three groups of 50 cutting shoots are arranged in parallel; the average values of rooting time, survival rate and seedling time of each group were observed, as shown in table 6 below.
TABLE 6 rooting of lufa 50 cuttings in different examples
| Method | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | CK |
| Rooting time/day | 7.5 | 8.6 | 7.7 | 7.2 | 6.5 | 15.3 |
| Survival rate | 92.6 | 94.4 | 97.3 | 95.2 | 99.1 | 60.4 |
| Seedling time/day | 23.4 | 25.8 | 22.6 | 20.7 | 18.3 | 80.6 |
As can be seen from the above table 6, the method provided by the embodiment of the invention can improve the cutting survival rate of the lumao 50 twig, is short in rooting time, greatly shortens the seedling time, and improves the propagation efficiency of the lumao 50.
(6) Influence of different conditions on survival rate of transplanted RuMao 50
Selecting 50 lumao twig cuttings, and performing cutting seedling culture according to the methods of the embodiments 1-5 and the control groups 1-4 respectively, wherein each group comprises 20 cuttings, and three groups are arranged in parallel; the survival rate of the lumao 50 after each group of transplantation was observed.
The control group was as follows:
control group 1: after 18 days of cutting, transplanting was carried out directly, and the other steps were the same as in example 5.
Control group 2: after 10 days of cutting, the seedling raising tray was moved to another greenhouse, and the other steps were the same as in example 5.
Control group 3: after the transfer to another greenhouse, the temperature of the greenhouse was controlled at 35 ℃, and the other steps were the same as in example 5.
Control group 4: and (3): inserting the cutting slips obtained in the step (1) into a matrix, and spraying water for one time to ensure that the cutting slips are fully contacted with the matrix; the temperature was maintained at 43 ℃ and the humidity in the greenhouse was controlled at 90%, and the other steps were the same as in example 5.
The specific results are shown in table 7 below.
TABLE 7 survival rates after transplantation of luggu 50 of various examples and controls
As can be seen from table 7 above, the survival rate of the rooted seedlings of the lufa 50 twigs transplanted by the methods of examples 1 to 5 is much higher than that of the control group, which indicates that the direct transplantation is not favorable for the survival of the lufa 50 after the cutting rooting of the lufa 50 twigs is promoted under the high-temperature and high-humidity environment condition; the survival rate of the transplanted rufa 50 can be influenced by the greenhouse turning time and the temperature and humidity in the greenhouse after the greenhouse is turned.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (12)
1. A method for raising seedlings of Chinese white poplar twig by cutting is characterized by comprising the following steps:
(1) collecting the young branches of the annual strong and semi-lignified male Chinese white poplar as cuttings, and cutting the young branches into cuttings; soaking the lower end of the cutting with a rooting agent;
the rooting agent is an indolebutyric acid solution, and the lower end of the cutting is soaked in the rooting agent for 0.5-3 hours;
the concentration of the indolebutyric acid solution is 90-150 mg/L;
(2) sterilizing the cutting substrate;
the matrix consists of crushed coconut coir, perlite and vermiculite according to a mass ratio of 60-80: 15: 15;
(3) inserting the cutting slips obtained in the step (1) into a sterilized cutting medium, and spraying water thoroughly for one time to ensure that the cutting slips are fully contacted with the medium; controlling the temperature at 37-45 ℃ in the daytime and 27-30 ℃ at night, and controlling the humidity in the shed at 85-90%;
(4) disinfecting the leaf surfaces every 2-3 days;
(5) after 9-10 days of cuttage, the cutting shoots begin to root, and foliar fertilizer is sprayed once every 2-3 days;
(6) hardening off seedlings 15-20 days after cuttage, shading the rooted seedlings, culturing for 3-5 days, gradually ventilating and exposing to light, controlling the temperature at 25-30 ℃ and the humidity at 50-70%; disinfecting the leaf surfaces every 3-4 days, and spraying leaf fertilizer every 2-3 days; and (5) transplanting the seedlings after 8-12 days of cultivation.
2. The method as claimed in claim 1, wherein the step (1) is to cut the shoots into 12-20 cm cuttings.
3. The method according to claim 1, wherein the concentration of the indolebutyric acid solution of step (1) is 95-110 mg/L.
4. The method according to claim 3, wherein the concentration of the solution of indole butyric acid of step (1) is 100 mg/L.
5. The method of claim 1, wherein step (1) retains half a leaf per cutting.
6. The method of claim 1, wherein the substrate of step (2) is sterilized with a carbendazim solution.
7. The method according to claim 6, wherein the matrix consists of coconut coir, perlite and vermiculite in a mass ratio of 70:15: 15.
8. The method according to claim 1, wherein the temperature of step (3) during the day is controlled to be 40-45 ℃.
9. The method of claim 8, wherein the temperature of step (3) is controlled at 43 ℃ during the day.
10. The method as claimed in claim 1, wherein the night temperature in step (3) is controlled at 28 ℃.
11. The method of claim 1, wherein the male aspen is luvit 50.
12. The method according to any one of claims 1 to 11, wherein the seedling cutting time is from 6 to 7 months.
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| CN113785707A (en) * | 2021-09-26 | 2021-12-14 | 连云港西诺花卉种业有限公司 | Method for increasing summer cuttage survival rate of cerasus sessilifolia |
| CN114190176A (en) * | 2021-12-09 | 2022-03-18 | 李玉晏 | Cuttage method for larch |
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| CN107371706A (en) * | 2017-08-01 | 2017-11-24 | 四川百绿盛三生农业科技有限公司 | A kind of cinnamomum kanehirai cuttage and seedling culture method |
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