CN109793009A - It is a kind of to walk quickly and keep away pharmaceutical preparation for prevent and treat TYLCV infection insect - Google Patents
It is a kind of to walk quickly and keep away pharmaceutical preparation for prevent and treat TYLCV infection insect Download PDFInfo
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- CN109793009A CN109793009A CN201910228361.4A CN201910228361A CN109793009A CN 109793009 A CN109793009 A CN 109793009A CN 201910228361 A CN201910228361 A CN 201910228361A CN 109793009 A CN109793009 A CN 109793009A
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- smelly liquid
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Abstract
Pharmaceutical preparation is walked quickly and kept away for prevent and treat TYLCV infection insect the invention discloses a kind of, the following steps are included: step 1: the Tessaratoma Papillosa Drury adult of artificial breeding is immersed in physiological saline, the smelly liquid in the scent gland of Tessaratoma Papillosa Drury adult is discharged in heating, filter impurities in water, ethanol solution is added, heating and refluxing extraction obtains the smelly liquid aqueous solution of Tessaratoma Papillosa Drury；Step 2: and then paris polyphylla leaf and lobelia chinensis are added into the smelly liquid aqueous solution of Tessaratoma Papillosa Drury, by mixed solution centrifuging and taking supernatant after hyperbaric heating, removing toxic substances enzyme reaction is added into supernatant, macroreticular resin on supernatant is taken to decolourize；Step 3: sequentially adding thickener, MMB, surfactant in except the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison, be evaporated under reduced pressure after mixing, and emulsifier is added and obtains ointment, pelletizer on ointment is granulated, obtains walking quickly and keeping away drug after spray drying.
Description
Technical field
The present invention relates to technical field of biological extraction, are specifically related to a kind of for preventing and treating walking quickly and keeping away for TYLCV infection insect
Pharmaceutical preparation.
Background technique
Tomato be the most commonly used vegetable variety of the current cultivated area in China and our most important vegetable species it
One.The annual cultivated area in the whole nation reaches 800,000 hm since 2015 according to statistics2, account for about the 4.5% of national sowing of vegetable area.Kind
Eggplant tomato yellow leaf curl China virus (TYLCV) is the tomato destructive disease occurred recent years, and the virus Yangtze river basin in 2006 originates
Raw, north area sprawling occurs rapidly later, present Shandong, Hebei, Jiangsu, Henan, Beijing, Xinjiang, southern Liaoning Province
Have been found that tomato yellow leaf curl virus disease, which is to endanger disease the most serious to tomato at present.
The tomato plant for infecting tomato yellow leaf curl China viral disease is downgraded, slow growth or stagnation, top vane often slightly chlorisis jaundice,
Become smaller, blade thickness, leaf roll qualitative change is hard on edge, and leaf back vein often shows purple.Growth and development early stage infected plant is seriously short
Contracting, only upper leaf and sprouting performance symptom, number of results are reduced the growth and development later period infected plant that can not normally yield positive results, fruit
Become smaller, maturity period fruit color is uneven (red impermeable), loses commodity control measure substantially.
Studies have found that the substance splash that Bemisia tabaci is detested can be walked quickly and kept away infection insect Bemisia tabaci on tomato plant
Feeding, thus achieve the purpose that prevent and treat tomato yellow leaf curl virus disease, so, it designs a kind of for preventing and treating TYLCV infection insect
Walk quickly and keep away pharmaceutical preparation very it is necessary to.
Summary of the invention
In view of the above problems, drug system is walked quickly and kept away for prevent and treat TYLCV infection insect the present invention provides a kind of
Standby technique.
The technical scheme is that it is a kind of for preventing and treating the pharmaceutical preparation of walking quickly and keeping away of TYLCV infection insect, including with
Lower step:
Step 1: smelly liquid extracts
The Tessaratoma Papillosa Drury adult of artificial breeding is immersed in physiological saline, is risen water temperature with the heating rate of 5 DEG C/min
To 60-65 DEG C, the smelly liquid in scent gland is discharged when water temperature gradually rises for the scent gland of Tessaratoma Papillosa Drury adult, is kept for temperature 15-20 points
Clock pulls Tessaratoma Papillosa Drury adult out, filtering impurities in water, the ethanol solution of 75% concentration of addition, and heating and refluxing extraction four times, by four
Secondary extracting solution obtains the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after merging recycling ethyl alcohol；
Step 2: except poison concentration
Adjust the smelly liquid aqueous solution pH to 6.8-7.8 of Tessaratoma Papillosa Drury, then into the smelly liquid aqueous solution of Tessaratoma Papillosa Drury be added paris polyphylla leaf and
Detoxication enzyme is added into supernatant, sets by mixed solution centrifuging and taking supernatant after being cooled to room temperature by lobelia chinensis, hyperbaric heating 2h
Reaction 1-2d is stood at a temperature of 37-40 DEG C, centrifuging and taking supernatant, upper macroreticular resin are removed after decolourizing again by enzymatic hydrolysis solution
The smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison；
Step 3: medicine preparation is walked quickly and kept away
Thickener, MMB, surfactant are sequentially added in except the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison, it is mixed with mixer
Close 1-2h and obtain mixed solution, be evaporated under reduced pressure by mixed solution be evaporated to it is thick obtain ointment, pelletizer on ointment is carried out
It is granulated, after granulated drug drying, obtains walking quickly and keeping away drug.
Further, the thickener is hydroxyethyl cellulose, hydroxypropyl methyl cellulose, sodium alginate, carbomer
In two or more mixtures, thickener can improve the viscosity of solution, convenient for being granulated.
Further, the surfactant is one of lecithin, fatty glyceride, neopelex
Or two or more mixtures, surfactant, which is added, to be made to walk quickly and keep away the readily volatilized smell of drug, causes avoidance effect to Bemisia tabaci.
Further, four times being heated to reflux are followed successively by 2h, 2h, 1.5h, 1h in the step 1,.
Further, the actual conditions of the step 2 mesohigh heating are as follows: 180-200 DEG C of temperature, pressure 20-30kPa,
High temperature and pressure can both kill the noxious material in the smelly liquid aqueous solution of Tessaratoma Papillosa Drury, can also make the solution in paris polyphylla leaf and lobelia chinensis
Malicious ingredient is sufficiently reacted with the smelly liquid of Tessaratoma Papillosa Drury.
Further, the detoxication enzyme is glutathione-S-transferase, and glutathione-S-transferase is to the smelly liquid of Tessaratoma Papillosa Drury
In toxic component have good decomposition.
Further, the drug of walking quickly and keeping away can directly spread to tomato plant root or directly be sprayed to after diluting 60-70 times
On tomato plant, solid walks quickly and keeps away after drug is spread to tomato plant root and dilution and is sprayed directly on tomato plant and can persistently distribute
The smell of the smelly liquid of Tessaratoma Papillosa Drury, Bemisia tabaci is driven, and will be dispelled in the smelly liquid of Tessaratoma Papillosa Drury to the harmful ingredient of tomato plant, is avoided
Tomato plant is damaged.
The beneficial effects of the present invention are: provided by the invention a kind of drug system is walked quickly and kept away for prevent and treat TYLCV infection insect
Standby technique, compared with the existing technology in pass through and plant the plants such as celery, capsicum, the smell distributed by plant between tomato plant
It walks quickly and keeps away for the means of Bemisia tabaci, the present invention extracts except poison its smelly liquid, removing it by the smelly liquid of extraction Tessaratoma Papillosa Drury
It goes on the basis of noxious material to retain stink, Bemisia tabaci is walked quickly and kept away by the stink of the smelly liquid of Tessaratoma Papillosa Drury, passes through experimental study
It was found that the avoidance effect of the smelly liquid of Tessaratoma Papillosa Drury is significantly greater than the avoidance effect of the plants such as celery, capsicum, and keeps away drug and can directly spread
It is directly sprayed on tomato plant after to 60-70 times of tomato plant root or dilution, it is easy to use.
Specific embodiment
For convenient for the understanding to technical solution of the present invention, the present invention will be further explained combined with specific embodiments below
Illustrate, embodiment does not constitute the restriction to invention protection scope.
Embodiment 1
It is a kind of to walk quickly and keep away pharmaceutical preparation for prevent and treat TYLCV infection insect, comprising the following steps:
Step 1: smelly liquid extracts
The Tessaratoma Papillosa Drury adult of artificial breeding is immersed in the physiological saline for not crossing Tessaratoma Papillosa Drury adult, with the liter of 5 DEG C/min
Water temperature is risen to 60 DEG C by warm rate, and the smelly liquid in scent gland is discharged when water temperature gradually rises for the scent gland of Tessaratoma Papillosa Drury adult, is protected
It holds temperature 15 minutes, Tessaratoma Papillosa Drury adult is pulled out, filter impurities in water, the second of 75% concentration of physiological saline half quality is added
Alcoholic solution, heating and refluxing extraction four times, four times being heated to reflux are followed successively by 2h, 2h, 1.5h, 1h, and No. four extracting solutions are closed
And the smelly liquid aqueous solution of Tessaratoma Papillosa Drury is obtained after recycling ethyl alcohol；
Step 2: except poison concentration
The smelly liquid aqueous solution pH to 6.8 of Tessaratoma Papillosa Drury is adjusted, paris polyphylla leaf and one side of something are then added into the smelly liquid aqueous solution of Tessaratoma Papillosa Drury
Lotus, paris polyphylla leaf is identical with lobelia chinensis additional amount, paris polyphylla leaf and lobelia chinensis total quality be the smelly liquid aqueous solution of Tessaratoma Papillosa Drury ten/
One, hyperbaric heating 2h under the conditions of 180 DEG C of temperature, pressure 20kPa, by mixed solution centrifuging and taking supernatant after being cooled to room temperature, to
The detoxication enzyme with quality such as paris polyphylla leaf and lobelia chinensis is added in supernatant, reaction 1d is stood at a temperature of being placed in 37 DEG C, will be digested molten
Centrifuging and taking supernatant, upper macroreticular resin obtain after decolourizing except the Tessaratoma Papillosa Drury smelly liquid aqueous solution after poison liquid again；
Step 3: medicine preparation is walked quickly and kept away
Sequentially add thickener, MMB, surfactant in except the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison, thickener, MMB,
The mass ratio of surfactant be 2:1:3, thickener, MMB, surfactant total quality be the smelly liquid aqueous solution matter of Tessaratoma Papillosa Drury
The one third of amount obtains mixed solution with mixer mixing 1h, is evaporated under reduced pressure for mixed solution to be evaporated to and thick obtains medicine
Pelletizer on ointment is granulated by cream, by granulated drug it is dry after, obtain walking quickly and keeping away drug, walk quickly and keep away drug can directly spread to kind
It is directly sprayed on tomato plant after 60 times of eggplant plant root or dilution；
Thickener is the mixture of hydroxyethyl cellulose, hydroxypropyl methyl cellulose, sodium alginate；Surfactant is ovum
The mixture of phosphatide, fatty glyceride；Detoxication enzyme is glutathione-S-transferase.
Embodiment 2
It is a kind of to walk quickly and keep away pharmaceutical preparation for prevent and treat TYLCV infection insect, comprising the following steps:
Step 1: smelly liquid extracts
The Tessaratoma Papillosa Drury adult of artificial breeding is immersed in the physiological saline for not crossing Tessaratoma Papillosa Drury adult, with the liter of 5 DEG C/min
Water temperature is risen to 65 DEG C by warm rate, and the smelly liquid in scent gland is discharged when water temperature gradually rises for the scent gland of Tessaratoma Papillosa Drury adult, is protected
It holds temperature 18 minutes, Tessaratoma Papillosa Drury adult is pulled out, filter impurities in water, the second of 75% concentration of physiological saline half quality is added
Alcoholic solution, heating and refluxing extraction four times, four times being heated to reflux are followed successively by 2h, 2h, 1.5h, 1h, and No. four extracting solutions are closed
And the smelly liquid aqueous solution of Tessaratoma Papillosa Drury is obtained after recycling ethyl alcohol；
Step 2: except poison concentration
The smelly liquid aqueous solution pH to 7.3 of Tessaratoma Papillosa Drury is adjusted, paris polyphylla leaf and one side of something are then added into the smelly liquid aqueous solution of Tessaratoma Papillosa Drury
Lotus, paris polyphylla leaf is identical with lobelia chinensis additional amount, paris polyphylla leaf and lobelia chinensis total quality be the smelly liquid aqueous solution of Tessaratoma Papillosa Drury ten/
One, hyperbaric heating 2h under the conditions of 190 DEG C of temperature, pressure 25kPa, by mixed solution centrifuging and taking supernatant after being cooled to room temperature, to
The detoxication enzyme with quality such as paris polyphylla leaf and lobelia chinensis is added in supernatant, reaction 1.5d is stood at a temperature of being placed in 38 DEG C, will be digested
Centrifuging and taking supernatant, upper macroreticular resin obtain after decolourizing except the Tessaratoma Papillosa Drury smelly liquid aqueous solution after poison solution again；
Step 3: medicine preparation is walked quickly and kept away
Sequentially add thickener, MMB, surfactant in except the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison, thickener, MMB,
The mass ratio of surfactant be 2:1:3, thickener, MMB, surfactant total quality be the smelly liquid aqueous solution matter of Tessaratoma Papillosa Drury
The one third of amount obtains mixed solution with mixer mixing 1.5h, is evaporated under reduced pressure mixed solution being evaporated to thick obtain
Pelletizer on ointment is granulated by ointment, by granulated drug it is dry after, obtain walking quickly and keeping away drug, walk quickly and keep away drug can directly spread to
It is directly sprayed on tomato plant after 65 times of tomato plant root or dilution；
Thickener is the mixture of hydroxyethyl cellulose, hydroxypropyl methyl cellulose, carbomer；Surfactant is ovum
The mixture of phosphatide, neopelex；Detoxication enzyme is glutathione-S-transferase.
Embodiment 3
It is a kind of to walk quickly and keep away pharmaceutical preparation for prevent and treat TYLCV infection insect, comprising the following steps:
Step 1: smelly liquid extracts
The Tessaratoma Papillosa Drury adult of artificial breeding is immersed in the physiological saline for not crossing Tessaratoma Papillosa Drury adult, with the liter of 5 DEG C/min
Water temperature is risen to 65 DEG C by warm rate, and the smelly liquid in scent gland is discharged when water temperature gradually rises for the scent gland of Tessaratoma Papillosa Drury adult, is protected
It holds temperature 20 minutes, Tessaratoma Papillosa Drury adult is pulled out, filter impurities in water, the second of 75% concentration of physiological saline half quality is added
Alcoholic solution, heating and refluxing extraction four times, four times being heated to reflux are followed successively by 2h, 2h, 1.5h, 1h, and No. four extracting solutions are closed
And the smelly liquid aqueous solution of Tessaratoma Papillosa Drury is obtained after recycling ethyl alcohol；
Step 2: except poison concentration
The smelly liquid aqueous solution pH to 7.8 of Tessaratoma Papillosa Drury is adjusted, paris polyphylla leaf and one side of something are then added into the smelly liquid aqueous solution of Tessaratoma Papillosa Drury
Lotus, paris polyphylla leaf is identical with lobelia chinensis additional amount, paris polyphylla leaf and lobelia chinensis total quality be the smelly liquid aqueous solution of Tessaratoma Papillosa Drury ten/
One, hyperbaric heating 2h under the conditions of 200 DEG C of temperature, pressure 30kPa, by mixed solution centrifuging and taking supernatant after being cooled to room temperature, to
The detoxication enzyme with quality such as paris polyphylla leaf and lobelia chinensis is added in supernatant, reaction 2d is stood at a temperature of being placed in 40 DEG C, will be digested molten
Centrifuging and taking supernatant, upper macroreticular resin obtain after decolourizing except the Tessaratoma Papillosa Drury smelly liquid aqueous solution after poison liquid again；
Step 3: medicine preparation is walked quickly and kept away
Sequentially add thickener, MMB, surfactant in except the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison, thickener, MMB,
The mass ratio of surfactant be 2:1:3, thickener, MMB, surfactant total quality be the smelly liquid aqueous solution matter of Tessaratoma Papillosa Drury
The one third of amount obtains mixed solution with mixer mixing 2h, is evaporated under reduced pressure for mixed solution to be evaporated to and thick obtains medicine
Pelletizer on ointment is granulated by cream, by granulated drug it is dry after, obtain walking quickly and keeping away drug, walk quickly and keep away drug can directly spread to kind
It is directly sprayed on tomato plant after 70 times of eggplant plant root or dilution；
Thickener is the mixture of hydroxyethyl cellulose, hydroxypropyl methyl cellulose, sodium alginate, carbomer；Surface
Activating agent is the mixture of lecithin, fatty glyceride, neopelex；Detoxication enzyme is glutathione-S- transfer
Enzyme.
Experimental example 4
Experimental subjects: the planting site of five pieces of homalographics of selection plants the susceptible tomato plant of 100 same breeds respectively,
Five pieces of ground are two-by-two at a distance of being no more than 1km, and the illumination on four pieces of ground, edaphic condition are identical.
Experiment condition: by embodiment 1 prepare walk quickly and keep away 60 times of drug dilution after be uniformly sprayed at first piece of ground tomato plant
In strain, by embodiment 2 prepare walk quickly and keep away 65 times of drug dilution after be uniformly sprayed on the tomato plant on second piece of ground, by embodiment
3 preparation walk quickly and keep away 70 times of drug dilution after be uniformly sprayed on the tomato plant on third block ground, walk quickly and keep away medicine for prepared by embodiment 3
Object is directly spread to the tomato plant root on the 4th piece of ground, and the 5th piece of ground is as blank control group.
Experimental result: the tomato plant infection chrysanthemum leaf curl disease on four pieces of ground is observed after 10d, 15d, 20d, 30d
The tomato plant quantity of poison, the results are shown in Table 1.
1 tomato plant of table infects chrysanthemum leaf curl virus results table
| 10d | 15d | 20d | 30d | |
| First piece of ground | 0 | 1 | 2 | 8 |
| Second piece of ground | 0 | 1 | 1 | 4 |
| Third block | 0 | 0 | 0 | 2 |
| 4th piece of ground | 0 | 0 | 2 | 5 |
| 5th piece of ground | 0 | 39 | 91 | 100 |
Conclusion: technique preparation provided by the invention walks quickly and keeps away drug for walking quickly and keeping away Bemisia tabaci to carry out prevention and treatment tomato yellowing song
Mosaic virus is really effective, embodiment 3 prepare to walk quickly and keep away effect of drugs optimal, and drug will be walked quickly and kept away and directly spread to tomato plant root
Effect is better than being sprayed on tomato plant after walking quickly and keeping away drug dilution.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify to technical solution documented by previous embodiment or equivalent replacement of some of the technical features；And
These are modified or replaceed, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (7)
1. a kind of walk quickly and keep away pharmaceutical preparation for prevent and treat TYLCV infection insect, which comprises the following steps:
Step 1: smelly liquid extracts
The Tessaratoma Papillosa Drury adult of artificial breeding is immersed in physiological saline, water temperature is risen to by 60- with the heating rate of 5 DEG C/min
65 DEG C, the smelly liquid in scent gland is discharged when water temperature gradually rises for the scent gland of Tessaratoma Papillosa Drury adult, is kept for temperature 15-20 minutes, will
Tessaratoma Papillosa Drury adult is pulled out, and impurities in water is filtered, and the ethanol solution of 75% concentration is added, heating and refluxing extraction four times, four times are mentioned
The smelly liquid aqueous solution of Tessaratoma Papillosa Drury is obtained after taking liquid to merge recycling ethyl alcohol；
Step 2: except poison concentration
The smelly liquid aqueous solution pH to 6.8-7.8 of Tessaratoma Papillosa Drury is adjusted, paris polyphylla leaf and one side of something are then added into the smelly liquid aqueous solution of Tessaratoma Papillosa Drury
Detoxication enzyme is added into supernatant, is placed in 37- by mixed solution centrifuging and taking supernatant after being cooled to room temperature by lotus, hyperbaric heating 2h
Reaction 1-2d is stood at a temperature of 40 DEG C, centrifuging and taking supernatant, upper macroreticular resin are obtained after decolourizing except after poison again by enzymatic hydrolysis solution
The smelly liquid aqueous solution of Tessaratoma Papillosa Drury；
Step 3: medicine preparation is walked quickly and kept away
Thickener, MMB, surfactant are sequentially added in except the smelly liquid aqueous solution of Tessaratoma Papillosa Drury after poison, with mixer mixing 1-
2h obtains mixed solution, be evaporated under reduced pressure by mixed solution be evaporated to it is thick obtain ointment, pelletizer on ointment is granulated,
After granulated drug drying, obtain walking quickly and keeping away drug.
2. according to claim 1 a kind of for preventing and treating the pharmaceutical preparation of walking quickly and keeping away of TYLCV infection insect, feature exists
In, the thickener be hydroxyethyl cellulose, it is hydroxypropyl methyl cellulose, sodium alginate, two or more mixed in carbomer
Close object.
3. according to claim 1 a kind of for preventing and treating the pharmaceutical preparation of walking quickly and keeping away of TYLCV infection insect, feature exists
In the surfactant is the mixing of one or more of lecithin, fatty glyceride, neopelex
Object.
4. according to claim 1 a kind of for preventing and treating the pharmaceutical preparation of walking quickly and keeping away of TYLCV infection insect, feature exists
In four times being heated to reflux are followed successively by 2h, 2h, 1.5h, 1h in the step 1.
5. according to claim 1 a kind of for preventing and treating the pharmaceutical preparation of walking quickly and keeping away of TYLCV infection insect, feature exists
In the actual conditions of the step 2 mesohigh heating are as follows: 180-200 DEG C of temperature, pressure 20-30kPa.
6. walking quickly and keeping away drug using the preparation of preparation process described in claim 1-5 any one, which is characterized in that walked quickly and kept away described
Drug is directly sprayed on tomato plant after can directly spreading to 60-70 times of tomato plant root or dilution.
7. walking quickly and keeping away drug using the preparation of preparation process described in claim 1-5 any one, which is characterized in that walked quickly and kept away described
It is directly sprayed on tomato plant after 60-70 times of drug dilution.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016166167A (en) * | 2015-03-05 | 2016-09-15 | キッコーマン株式会社 | Control method of plant viral disease transmission |
| CN107156195A (en) * | 2017-07-13 | 2017-09-15 | 临沂市农业科学院 | A kind of botanical pesticide for preventing and treating plant insect and its application |
-
2019
- 2019-03-25 CN CN201910228361.4A patent/CN109793009A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016166167A (en) * | 2015-03-05 | 2016-09-15 | キッコーマン株式会社 | Control method of plant viral disease transmission |
| CN107156195A (en) * | 2017-07-13 | 2017-09-15 | 临沂市农业科学院 | A kind of botanical pesticide for preventing and treating plant insect and its application |
Non-Patent Citations (1)
| Title |
|---|
| 江军: "荔枝蝽臭腺提取物的杀虫活性及其熏蒸作用机理研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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