CN109777736B - Living cell analysis equipment and method - Google Patents
Living cell analysis equipment and method Download PDFInfo
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- CN109777736B CN109777736B CN201910087095.8A CN201910087095A CN109777736B CN 109777736 B CN109777736 B CN 109777736B CN 201910087095 A CN201910087095 A CN 201910087095A CN 109777736 B CN109777736 B CN 109777736B
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- 238000004458 analytical method Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 62
- 239000001301 oxygen Substances 0.000 claims abstract description 62
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 62
- 230000004083 survival effect Effects 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims abstract description 8
- 230000003204 osmotic effect Effects 0.000 claims abstract description 6
- 238000002474 experimental method Methods 0.000 claims description 119
- 238000012360 testing method Methods 0.000 claims description 48
- 239000003153 chemical reaction reagent Substances 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 16
- 230000035899 viability Effects 0.000 claims description 16
- 230000007246 mechanism Effects 0.000 claims description 7
- 238000004113 cell culture Methods 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims 1
- 230000003712 anti-aging effect Effects 0.000 abstract description 16
- 239000002537 cosmetic Substances 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 61
- 230000008439 repair process Effects 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000004623 platelet-rich plasma Anatomy 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 230000001737 promoting effect Effects 0.000 description 1
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- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses living cell analysis equipment and a living cell analysis method, and particularly relates to the field of living cell analysis. The invention utilizes the PLC to control the work of the pressure pump and the opening of the flow control valve, respectively detects the optimal living environment of the autologous living cells under different osmotic pressure, oxygen and temperature environments, cultures the autologous living cells with complete functions and highest survival rate, is used for the cosmetic industry, can ensure that the anti-aging effect is better, and the cells with strong anti-aging and repairing capability can improve the anti-aging and repairing effects of users.
Description
Technical Field
The present invention relates to the field of living cell analysis technology, and more particularly, to a living cell analysis apparatus and method.
Background
The autologous living cells are based on PRP, the PRP is an abbreviation of Platelet-rich plasma, the name of the PRP is high-concentration Platelet plasma, the PRP is high-concentration Platelet-rich plasma prepared by utilizing self blood, the principle of PRP anti-aging repair is realized, and the PRP is formed by promoting the skin to regenerate according to the strong regeneration force of growth factors;
the American Essen corporation developed a third generation of Long-time real-time dynamic living cell imaging analyzer, IncuCyte S3, which was used to record the real-time growth status of cells by a non-invasive method. This Imaging method, known as "real-time cellular Content Imaging," extends the way users record and understand cell growth, cell behavior, and cell morphology.
However, this instrument also has the following disadvantages: the volume is little, and the environment is single, and the board, the ware, bottle and the slide glass that detect living cell place limitedly, can not observe a plurality of living cells survival state under different living environment simultaneously, and the cell contrasts in unable a plurality of cultivators, and contrast result can not audio-visually clear show, is unfavorable for the later stage and cultivates.
Disclosure of Invention
In order to overcome the above defects in the prior art, embodiments of the present invention provide a living cell analysis apparatus and method, in which a PLC controller is used to control the operation of a pressure pump and the opening of a flow control valve, so as to respectively change the culture medium concentration in a culture medium concentration test box, the oxygen concentration in an oxygen concentration test box, and the temperature in a temperature test box, respectively detect the optimal environments in which living autologous cells live under different osmotic pressure, oxygen, and temperature environments, respectively, and culture autologous living cells with complete functions and the highest survival rate, for use in the cosmetic industry, which can improve the anti-aging effect and the repairing effect of users with strong anti-aging repairing ability.
In order to achieve the purpose, the invention provides the following technical scheme: a living cell analysis device comprises a machine body, wherein a feeding bin and an experiment bin are arranged in the machine body, the experiment bin is arranged at the bottom of the feeding bin, a water tank, a culture solution tank, a fluorescent reagent tank and an oxygen tank are arranged in the feeding bin, a rotary experiment box is arranged in the experiment bin, the rotary experiment box is arranged into three layers, a driving mechanism is arranged outside each layer of rotary experiment box, a conveying main pipe is fixedly sleeved at the center inside each layer of rotary experiment box, a plurality of experiment cabinets are uniformly arranged inside the rotary experiment box corresponding to the outer side of the conveying main pipe in a surrounding manner, a movable sealing door is arranged at the front side of each experiment cabinet, and a culture dish, a camera, an ultraviolet irradiation lamp and a temperature control lamp are arranged in each experiment cabinet;
conveying house steward top and bottom all are equipped with ball bearing, the inside distributing pipe that is equipped with of conveying house steward, water tank, culture solution jar, fluorescence reagent jar and oxygen cylinder all loop through force pump, defeated material multi-ported tube and run through ball bearing and distributing pipe and be connected, the distributing pipe input runs through conveying house steward top and extends to inside the ball bearing, the distributing pipe output extends to the inside culture dish top of experiment cabinet, and is connected with flow control valve.
In a preferred embodiment, the water tank, the culture solution tank, the fluorescent reagent tank and the oxygen tank are arranged in the upper storage bin in a surrounding mode, and the top ends of the water tank, the culture solution tank, the fluorescent reagent tank and the oxygen tank penetrate through the machine body to be connected with the feeding top cover.
In a preferred embodiment, the driving mechanism comprises a stepping motor, a driving bevel gear and a driven bevel gear, the stepping motor is fixedly connected with the inner wall of the machine body, the driven bevel gear is fixedly arranged at the bottom of the rotary experiment box, and the driven bevel gear is perpendicular to the driving bevel gear and is in meshed connection with the driving bevel gear.
In a preferred embodiment, the three rotary test boxes are specifically a culture solution concentration test box, an oxygen concentration test box and a temperature test box from top to bottom, and the ball bearing between two adjacent rotary test boxes is fixedly connected with the machine body through a transverse plate.
In a preferred embodiment, an opening is formed in the machine body corresponding to the front side of each layer of the rotary experiment box, the length and the width of the opening are both greater than those of a movable sealing door on the experiment cabinet, the opening corresponds to the movable sealing door, and a handle is arranged on the movable sealing door.
In a preferred embodiment, the culture dish is arranged at the bottom of the inner cavity of the experiment cabinet, the camera is arranged at the top of the inner cavity of the experiment cabinet, and the ultraviolet irradiation lamp and the temperature control lamp are respectively arranged at two sides of the camera and fixedly connected with the experiment cabinet.
In a preferred embodiment, the distributing pipe comprises a vertical pipe and a plurality of transverse pipes connected to the bottom of the vertical pipe, the transverse pipes penetrate through the rear side wall of the experiment cabinet and extend to the upper side of the culture dish, the transverse pipes correspond to the experiment cabinets in the rotary experiment box respectively, the flow control valve is fixedly arranged on the transverse pipes, and spray heads are arranged at the end parts of the transverse pipes.
In a preferred embodiment, a touch display screen and a PLC controller are arranged on one side of the front side wall of the machine body, a display is arranged on the front side of the machine body corresponding to one side of each layer of the rotary experiment box, the PLC controller is connected with the display through an a/D converter, an image recognition module is arranged at the input end of the PLC controller, and the input end of the image recognition module is connected with the output end of the camera.
In a preferred embodiment, a rotary encoder is sleeved outside the conveying main pipe and is connected with a PLC controller through an A/D converter, and the PLC controller is connected with a stepping motor through a D/A converter.
The invention also provides a living cell analysis method, which specifically comprises the following steps:
firstly, respectively adding water, culture solution, a fluorescent reagent and oxygen into a water tank, a culture solution tank, a fluorescent reagent tank and an oxygen tank, then putting the culture solution with moderate concentration and an equivalent amount of autologous living cells into a culture dish, operating through a touch display screen, detecting a rotation angle by a rotary encoder, controlling a stepping motor to respectively drive a rotary experiment box on each layer to rotate, enabling each experiment cabinet to sequentially correspond to an opening, opening a movable sealing door, putting the culture dish, opening a temperature control lamp, ensuring certain illumination and keeping constant temperature;
in the second and third layers of rotary experiment boxes, a PLC controller controls a pressure pump on one side of an oxygen tank to work, a flow control valve is opened, oxygen is simultaneously conveyed into ball bearings in the third layer of rotary experiment boxes through a conveying multi-way pipe, and oxygen with proper concentration is conveyed into a plurality of experiment cabinets in each layer of rotary experiment box through a distributing pipe for cell culture;
in the oxygen concentration test box, a PLC controller is used for controlling a flow control valve on a distribution pipe in the oxygen concentration test box, so that the supply of different amounts of oxygen in each test cabinet in the oxygen concentration test box is realized, and the oxygen concentration in each test cabinet is ensured to be different;
in the culture solution concentration test box, a PLC controller controls a pressure pump on one side of a water tank to work, controls the opening time of a flow control valve, conveys water with different quantities to culture dishes in half of the experiment cabinets in the culture solution concentration test box through a distributing pipe, controls the pressure pump on one side of a culture solution tank to work after the conveyance is finished, conveys culture solution with different quantities to the culture dishes in the other half of the experiment cabinets, and ensures that the culture solution concentration in each experiment cabinet in the whole culture solution concentration test box is different;
in the temperature test box, a PLC controller controls a temperature control lamp in each test cabinet to adjust the temperature, so that different temperatures in each test cabinet are ensured;
step six, after autologous living cells are cultured for a period of time according to the environment in each layer of rotary experiment box, the PLC controls a pressure pump on one side of a fluorescent reagent tank to work, equal amounts of fluorescent reagents are conveyed into a culture dish in each experiment cabinet in each layer of rotary experiment box, then a camera and an ultraviolet irradiation lamp are turned on, a temperature control lamp is turned off, at the moment, the cells with strong viability can emit strong yellow-green fluorescence, and the cells with weak viability emit weaker fluorescence; dead cells do not have fluorescence, and according to the principle, the camera shoots the growth condition of the cells in each culture dish;
seventhly, pictures shot by the camera are sent to an image recognition module for recognition, the number of the culture dishes with strong viability and the number of the culture dishes with weak viability are calculated according to the fluorescence brightness, the obtained results are displayed by a display, the culture dishes with the highest number of cells with strong viability and the cell analysis data of the culture dishes are sent to a touch display screen through a PLC (programmable logic controller), the culture dish data displayed on the touch display screen and the data of the cell living environment where the culture dishes are located are called out by a worker, and the autologous living cells are cultured in the later period according to the data;
and step eight, before the culture, taking out and cleaning each culture dish, operating an ultraviolet irradiation lamp, sterilizing and disinfecting each experiment cabinet, and then culturing autologous living cells according to the data obtained in the step seven.
The invention has the technical effects and advantages that:
1. the invention controls the pressure pump connected with the water tank, the culture solution tank, the fluorescent reagent tank and the oxygen tank to work by utilizing the PLC controller and controls the opening of the flow control valve to respectively change the culture solution concentration in the culture solution concentration test box, the oxygen concentration in the oxygen concentration test box and the temperature in the temperature test box, respectively detect the optimal environment for the survival of autologous living cells under different osmotic pressure, oxygen and temperature environments, culture the autologous living cells with complete functions and highest survival rate for the cosmetic industry, the autologous living cells with strong vitality have better anti-aging effect, the cells with strong anti-aging repair capability can improve the anti-aging and repair effects of users, greatly improve the anti-aging capability, have strong vitality of the autologous living cells, and promote the skin to be regenerated according to the strong regeneration force of growth factors after the use, the later anti-aging effect time is long, and the maintenance time is longer;
2. the multiple experiment cabinets are arranged on each layer of the rotary experiment box, so that the osmotic pressure regulation, the oxygen concentration regulation and the temperature regulation variables are more, the experimental data are more detailed, the data comparison result is more reliable, the optimal environment for the survival of autologous living cells can be accurately obtained, a large number of cells can be conveniently cultured in the later period, the cell survival rate is highest, and when the anti-aging effect is used in the cosmetic industry, the anti-aging effect is more guaranteed and more reliable;
3. whole equipment intelligence operation is cultivateed and data detection, and intelligent accurate control culture solution, oxygen concentration and temperature avoid the error that manual operation leads to, influence the later stage and cultivate the result, utilize fluorescence labeling's method, the camera cooperates with image recognition module to use ultraviolet radiation lamp to provide the light source, detect the viability of cell according to the fluorescence luminance of cell, once measure and draw accurate result, data are reliable, reduce the experiment cost, provide the guarantee for the cultivation in a large number of later stages.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a top view of the upper bin of the present invention.
FIG. 3 is a top view of the rotary experimental box of the present invention.
FIG. 4 is a schematic structural diagram of the experimental cabinet of the present invention.
FIG. 5 is a schematic diagram of the system of the present invention.
The reference signs are: 1 organism, 2 feed bins, 3 experimental bins, 4 water tanks, 5 culture solution jars, 6 fluorescence reagent jar, 7 oxygen jar, 81 culture solution concentration test box, 82 oxygen concentration test box, 83 temperature test box, 9 actuating mechanism, 91 step motor, 92 initiative bevel gear, 93 driven bevel gear, 10 total conveying pipe, 11 experiment cabinets, 12 movable sealing door, 13 culture dishes, 14 cameras, 15 ultraviolet irradiation lamp, 16 temperature control lamp, 17 ball bearing, 18 distributing pipes, 19 force pumps, 20 conveying multi-way pipe, 21 flow control valve, 22 openings, 23 standpipe, 24 violently pipe, 25 touch display screen, 26 PLC controller, 27 display, 28 image recognition module, 29 rotary encoder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a living cell analysis apparatus shown in fig. 1-3, which includes a machine body 1, wherein an upper bin 2 and an experiment bin 3 are arranged inside the machine body 1, the experiment bin 3 is arranged at the bottom of the upper bin 2, a water tank 4, a culture solution tank 5, a fluorescent reagent tank 6 and an oxygen tank 7 are arranged inside the upper bin 2, a rotary experiment box is arranged inside the experiment bin 3, the rotary experiment box is arranged into three layers, a driving mechanism 9 is arranged outside each layer of the rotary experiment box, a main material conveying pipe 10 is fixedly sleeved in the center inside each layer of the rotary experiment box, a plurality of experiment cabinets 11 are uniformly arranged inside the rotary experiment box corresponding to the outside of the main material conveying pipe 10 in a surrounding manner, a movable sealing door 12 is arranged at the front side of each experiment cabinet 11, and a culture dish 13, a camera 14, an ultraviolet irradiation lamp 15 and a temperature control lamp 16 are arranged inside each experiment cabinet 11;
the three layers of the rotary experiment boxes are specifically a culture solution concentration test box 81, an oxygen concentration test box 82 and a temperature test box 83 from top to bottom, and the ball bearing 17 between two adjacent rotary experiment boxes is fixedly connected with the machine body 1 through a transverse plate;
the distributing pipe 18 comprises a vertical pipe 23 and a plurality of transverse pipes 24 connected to the bottom of the vertical pipe 23, the transverse pipes 24 penetrate through the rear side wall of the experiment cabinet 11 and extend to the upper part of the culture dish 13, the plurality of transverse pipes 24 respectively correspond to the plurality of experiment cabinets 11 in the rotary experiment box, the flow control valve 21 is fixedly arranged on the transverse pipes 24, and spray heads are arranged at the end parts of the transverse pipes 24;
the invention also provides a living cell analysis method, which specifically comprises the following steps:
firstly, respectively adding water, culture solution, fluorescent reagent and oxygen into a water tank 4, a culture solution tank 5, a fluorescent reagent tank 6 and an oxygen tank 7, then putting the culture solution with moderate concentration and an equal amount of autologous living cells into a culture dish 13, operating through a touch display screen 25, detecting a rotation angle by a rotary encoder 29, so as to control a stepping motor 91 to respectively drive a rotary experiment box on each layer to rotate, enabling each experiment cabinet 11 to sequentially correspond to an opening 22, opening a movable sealing door 12, putting the culture dish 13 in, opening a temperature control lamp 16, ensuring certain illumination and keeping constant temperature;
in the second and third layers of rotary experiment boxes, a PLC (programmable logic controller) 26 controls a pressure pump 19 on one side of an oxygen tank 7 to work, a flow control valve 21 is opened, oxygen is simultaneously conveyed into a ball bearing 17 in the third layer of rotary experiment box through a conveying multi-way pipe 20, and oxygen with proper concentration is conveyed into a plurality of experiment cabinets 11 in each layer of rotary experiment box through a distributing pipe 18 for cell culture;
step three, in the oxygen concentration test box 82, the flow control valve 21 on the distributing pipe 18 in the oxygen concentration test box 82 is controlled by the PLC 26, so that the supply of different amounts of oxygen in each experiment cabinet 11 in the oxygen concentration test box 82 is realized, and the oxygen concentration in each experiment cabinet 11 is ensured to be different;
in the culture solution concentration test box 81, the PLC 26 controls the pressure pump 19 on one side of the water tank 4 to work and controls the opening time of the flow control valve 21, water with different amounts is conveyed to the culture dishes 13 in half of the experiment cabinets 11 in the culture solution concentration test box 81 through the distributing pipe 18, after the conveyance is finished, the pressure pump 19 on one side of the culture solution tank 5 is controlled to work, culture solutions with different amounts are conveyed to the culture dishes 13 in the other half of the experiment cabinets 11, and the culture solution concentration in the culture dishes 13 in each experiment cabinet 11 in the whole culture solution concentration test box 81 is ensured to be different;
in the temperature test box 83, the PLC controller 26 controls the temperature control lamp 16 in each experiment cabinet 11 to adjust the temperature, so as to ensure that the temperature in each experiment cabinet 11 is different;
step six, after autologous living cells are cultured for a period of time according to the environment in each layer of rotary experiment box, the PLC 26 controls the pressure pump 19 on one side of the fluorescent reagent tank 6 to work, equal amount of fluorescent reagent is conveyed into the culture dish 13 in each experiment cabinet 11 in each layer of rotary experiment box, then the camera 14 and the ultraviolet irradiation lamp 15 are turned on, the temperature control lamp 16 is turned off, at this time, the cells with strong viability can emit strong yellow-green fluorescence, and the cells with weak viability emit weak fluorescence; dead cells are free of fluorescence, and according to the principle, the camera 14 shoots the growth condition of the cells in each culture dish 13;
seventhly, the photos shot by the camera 14 are sent to an image recognition module 28 for recognition, the number of strong viability and weak viability in each culture dish 13 is calculated according to the fluorescence brightness, the obtained results are displayed by a display 27, the culture dish 13 with the largest number of cells with strong viability and the cell analysis data of the culture dish are finally sent to a touch display screen 25 through a PLC (programmable logic controller) 26, a worker calls out the data of the culture dish 13 displayed on the touch display screen 25 and the data of the cell living environment where the culture dish 13 is located, and the autologous living cells are cultured in the later period according to the data;
and step eight, before the culture, taking out and cleaning each culture dish 13, operating the ultraviolet irradiation lamp 15, sterilizing and disinfecting each experiment cabinet 11, and then culturing autologous living cells according to the data obtained in the step seven.
Through the work of utilizing PLC controller 26 to control force pump 19 and the opening of flow control valve 21, change the culture solution concentration in culture solution concentration test case 81 respectively, the temperature in oxygen concentration and the temperature test case 83 in the oxygen concentration test case 82, detect respectively under different osmotic pressure, oxygen and temperature environment, the best environment that the autologous living cell lives, cultivate each item function perfect and the highest autologous living cell of survival rate, be used for the cosmetic trade, can make anti-aging effect better, cell that anti-aging repair ability is strong can improve user's anti-aging and repair effect.
Example 2:
according to the living cell analysis device and method shown in fig. 2, the water tank 4, the culture solution tank 5, the fluorescent reagent tank 6 and the oxygen tank 7 are arranged in the upper bin 2 in a surrounding manner, and the top ends of the tanks penetrate through the machine body 1 to be connected with a feeding top cover, so that feeding is facilitated;
an opening 22 is formed in the machine body 1 corresponding to the front side of each layer of the rotary experiment box, the length and the width of the opening 22 are both larger than those of the movable sealing door 12 on the experiment cabinet 11, the opening 22 corresponds to the movable sealing door 12, and a handle is arranged on the movable sealing door 12 to facilitate opening and taking/placing of the culture dish 13;
according to the living cell analysis apparatus and method shown in fig. 3, the driving mechanism 9 includes a stepping motor 91, a driving bevel gear 92 and a driven bevel gear 93, the stepping motor 91 is fixedly connected with the inner wall of the body 1, the driven bevel gear 93 is fixedly arranged at the bottom of the rotary experiment box, the driven bevel gear 93 is perpendicular to the driving bevel gear 92 and is meshed with the driving bevel gear 92, and the stepping motor 91 drives the driving bevel gear 92 to rotate through the driven bevel gear 93, so as to drive the rotary experiment box to rotate;
according to the living cell analysis equipment and method shown in fig. 4, the culture dish 13 is arranged at the bottom of the inner cavity of the experiment cabinet 11, the camera 14 is arranged at the top of the inner cavity of the experiment cabinet 11, the ultraviolet irradiation lamp 15 and the temperature control lamp 16 are respectively arranged at two sides of the camera 14 and are fixedly connected with the experiment cabinet 11, so that illumination and temperature are provided for the culture dish 13, a light source is provided for a fluorescent agent at the later stage, and sterilization and disinfection can be realized;
according to the living cell analysis apparatus and method shown in fig. 1 and 5, a touch display screen 25 and a PLC controller 26 are provided on one side of the front side wall of the body 1, a display 27 is provided on the front side of the body 1 corresponding to one side of each layer of the rotary experiment box, the PLC controller 26 is connected to the display 27 through an a/D converter, an image recognition module 28 is provided on the input end of the PLC controller 26, the input end of the image recognition module 28 is connected to the output end of the camera 14, the camera 14 sends the photographed image to the image recognition module 28, the image recognition module 28 recognizes, and calculates the number of strong and weak cells in each culture dish 13 according to the fluorescence brightness, the obtained result is displayed on the display 27, and the culture dish 13 with the highest number of strong cells and the cell analysis data thereof are sent to the touch display screen 25 through the PLC controller 26, the staff calls out the data of the culture dish 13 displayed on the touch display screen 25 and the data of the cell living environment where the culture dish 13 is located, and according to the data, the later-stage autologous living cell culture is carried out;
the outside cover of conveying main pipe 10 is equipped with rotary encoder 29, rotary encoder 29 passes through the AD converter and is connected with PLC controller 26, PLC controller 26 passes through the DA converter and is connected with step motor 91, detects rotation angle through rotary encoder 29 to through the turned angle of the whole rotatory experimental box of PLC controller 26 control, realize the last unloading of culture dish 13, and realize the accurate unloading of every culture dish 13.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (9)
1. A living cell analysis apparatus comprising a body (1), characterized in that: an upper material bin (2) and an experimental bin (3) are arranged in the machine body (1), the experimental bin (3) is arranged at the bottom of the upper bin (2), a water tank (4), a culture solution tank (5), a fluorescent reagent tank (6) and an oxygen tank (7) are arranged in the upper bin (2), the interior of the experiment bin (3) is provided with a rotary experiment box, the rotary experiment box is provided with three layers, the outer side of each layer of the rotary experiment box is provided with a driving mechanism (9), the center of the interior of each layer of the rotary experiment box is fixedly sleeved with a main material conveying pipe (10), a plurality of experimental cabinets (11) are uniformly arranged inside the rotary experimental box corresponding to the outer side of the main material conveying pipe (10) in a surrounding manner, a movable sealing door (12) is arranged on the front side of the experiment cabinet (11), and a culture dish (13), a camera (14), an ultraviolet irradiation lamp (15) and a temperature control lamp (16) are arranged in the experiment cabinet (11);
ball bearings (17) are arranged at the top end and the bottom end of the main material conveying pipe (10), a material distribution pipe (18) is arranged inside the main material conveying pipe (10), the water tank (4), the culture solution tank (5), the fluorescent reagent tank (6) and the oxygen tank (7) sequentially penetrate through the ball bearings (17) through a pressure pump (19) and a multi-way material conveying pipe (20) to be connected with the material distribution pipe (18), the input end of the material distribution pipe (18) penetrates through the top end of the main material conveying pipe (10) and extends into the ball bearings (17), and the output end of the material distribution pipe (18) extends to the upper portion of a culture dish (13) inside the experiment cabinet (11) and is connected with a flow control valve (21);
a touch display screen (25) and a PLC (programmable logic controller) (26) are arranged on one side of the front side wall of the machine body (1), a display (27) is arranged on the front side of the machine body (1) corresponding to one side of each layer of the rotary experiment box, the PLC (26) is connected with the display (27) through an A/D (analog/digital) converter, an image recognition module (28) is arranged at the input end of the PLC (26), and the input end of the image recognition module (28) is connected with the output end of the camera (14);
PLC controller (26) is used for controlling pressure pump (19) work that water tank (4), culture solution jar (5), fluorescence reagent jar (6) and oxygen jar (7) are connected to and control opening of flow control valve (21), change culture solution concentration in culture solution concentration test box (81) respectively, oxygen concentration in oxygen concentration test box (82) and the temperature in temperature test box (83), detect respectively under different osmotic pressure, oxygen and temperature environment, the best environment of autologous living cell survival, cultivate each item function perfect and the highest autologous living cell of survival rate.
2. The living cell analyzing apparatus according to claim 1, wherein: the water tank (4), the culture solution tank (5), the fluorescent reagent tank (6) and the oxygen tank (7) are arranged inside the upper storage bin (2) in a surrounding mode, and the top end of the fluorescent reagent tank penetrates through the machine body (1) to be connected with the feeding top cover.
3. The living cell analyzing apparatus according to claim 2, wherein: the driving mechanism (9) comprises a stepping motor (91), a driving bevel gear (92) and a driven bevel gear (93), the stepping motor (91) is fixedly connected with the inner wall of the machine body (1), the driven bevel gear (93) is fixedly arranged at the bottom of the rotary experiment box, and the driven bevel gear (93) and the driving bevel gear (92) are vertically arranged and are meshed with each other for connection.
4. The living cell analyzing apparatus according to claim 3, wherein: the three layers of the rotary experiment boxes are specifically a culture solution concentration experiment box (81), an oxygen concentration experiment box (82) and a temperature experiment box (83) from top to bottom, and a ball bearing (17) between every two adjacent rotary experiment boxes is fixedly connected with the machine body (1) through a transverse plate.
5. The living cell analyzing apparatus according to claim 4, wherein: every layer all be equipped with opening (22) on organism (1) that rotatory experimental box front side corresponds, opening (22) length and width all are greater than activity sealing door (12) length and width on experiment cabinet (11), opening (22) are corresponding with activity sealing door (12), be equipped with the handle on activity sealing door (12).
6. The living cell analyzing apparatus according to claim 5, wherein: the culture dish (13) is arranged at the bottom of an inner cavity of the experiment cabinet (11), the camera (14) is arranged at the top of the inner cavity of the experiment cabinet (11), and the ultraviolet irradiation lamp (15) and the temperature control lamp (16) are respectively arranged at two sides of the camera (14) and fixedly connected with the experiment cabinet (11).
7. The living cell analyzing apparatus according to claim 6, wherein: cloth pipe (18) include standpipe (23) and connect in a plurality of violently pipes (24) of standpipe (23) bottom, violently pipe (24) run through experiment cabinet (11) back lateral wall and extend to culture dish (13) top, and are a plurality of violently pipe (24) respectively with a plurality of experiment cabinets (11) in the rotatory experimental box corresponding, flow control valve (21) are fixed to be set up on violently pipe (24), violently pipe (24) tip is equipped with the shower nozzle.
8. The living cell analyzing apparatus according to claim 7, wherein: the outer cover of conveying main pipe (10) is equipped with rotary encoder (29), rotary encoder (29) are connected with PLC controller (26) through the AD converter, PLC controller (26) are connected with step motor (91) through the D/A converter.
9. A method for analyzing living cells, comprising: the method specifically comprises the following steps:
firstly, respectively adding water, culture solution, fluorescent reagent and oxygen into a water tank (4), a culture solution tank (5), a fluorescent reagent tank (6) and an oxygen tank (7), then putting the culture solution with moderate concentration and an equal amount of autologous living cells into a culture dish (13), operating through a touch display screen (25), detecting a rotation angle by a rotary encoder (29), controlling a stepping motor (91) to respectively drive a rotary experiment box of each layer to rotate, enabling each experiment cabinet (11) to sequentially correspond to an opening (22), opening a movable sealing door (12), putting the culture dish (13), opening a temperature control lamp (16), ensuring certain illumination and keeping constant temperature;
in the second and third layers of rotary experiment boxes, a PLC (26) controls a pressure pump (19) on one side of an oxygen tank (7) to work, a flow control valve (21) is opened, oxygen is simultaneously conveyed into a ball bearing (17) in the third layer of rotary experiment boxes through a material conveying multi-way pipe (20), and oxygen with a proper concentration is conveyed into a plurality of experiment cabinets (11) in each layer of rotary experiment box through a material distribution pipe (18) for cell culture;
in the oxygen concentration test box (82), a flow control valve (21) on a distributing pipe (18) in the oxygen concentration test box (82) is controlled through a PLC (programmable logic controller) (26), so that the supply of different amounts of oxygen in each experiment cabinet (11) in the oxygen concentration test box (82) is realized, and the oxygen concentration in each experiment cabinet (11) is ensured to be different;
in the culture solution concentration test box (81), a PLC (26) controls a pressure pump (19) on one side of a water tank (4) to work and controls the opening time of a flow control valve (21), water with different quantities is conveyed to culture dishes (13) in half of experiment cabinets (11) in the culture solution concentration test box (81) through a distributing pipe (18), after the conveying is finished, the pressure pump (19) on one side of a culture solution tank (5) is controlled to work, and culture solutions with different quantities are conveyed to the culture dishes (13) in the other half of the experiment cabinets (11), so that the culture solution concentration in each culture dish (13) in each experiment cabinet (11) in the whole culture solution concentration test box (81) is different;
in the temperature test box (83), the PLC (26) controls the temperature control lamp (16) in each experiment cabinet (11) to adjust the temperature, so as to ensure that the temperature in each experiment cabinet (11) is different;
step six, according to the environment in each layer of rotary experiment box, after autologous living cells are cultured for a period of time, a PLC (26) controls a pressure pump (19) on one side of a fluorescent reagent tank (6) to work, equal amounts of fluorescent reagents are conveyed into a culture dish (13) in each experiment cabinet (11) in each layer of rotary experiment box, then a camera (14) and an ultraviolet irradiation lamp (15) are started, a temperature control lamp (16) is turned off, at the moment, the cells with strong viability can emit strong yellow-green fluorescence, and the cells with weak viability emit weak fluorescence; dead cells are free of fluorescence, and according to the principle, a camera (14) shoots the growth condition of the cells in each culture dish (13);
seventhly, pictures shot by the camera (14) are sent to an image recognition module (28) for recognition, the number of strong viability and weak viability in each culture dish (13) is calculated according to the fluorescence brightness, the obtained results are displayed through a display (27), the culture dish (13) with the largest number of cells with strong viability and cell analysis data of the culture dish are sent to a touch display screen (25) through a PLC (programmable logic controller) (26), a worker calls out the data of the culture dish (13) displayed on the touch display screen (25) and the data of the cell living environment where the culture dish (13) is located, and autologous living cell culture in the later period is carried out according to the data;
and step eight, before the culture, taking out and cleaning each culture dish (13), operating an ultraviolet irradiation lamp (15), sterilizing and disinfecting each experiment cabinet (11), and then culturing autologous living cells according to the data obtained in the step seven.
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