CN109769950A - A kind of preparation method of delactose skimmed milk powder based on separation membrane technology - Google Patents
A kind of preparation method of delactose skimmed milk powder based on separation membrane technology Download PDFInfo
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- CN109769950A CN109769950A CN201910058850.XA CN201910058850A CN109769950A CN 109769950 A CN109769950 A CN 109769950A CN 201910058850 A CN201910058850 A CN 201910058850A CN 109769950 A CN109769950 A CN 109769950A
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Abstract
The invention discloses a kind of demulsification sugar skimmed milk power preparation methods based on separation membrane technology, pass through coupling ultrafiltration, electrodialysis, these three membrane technologies of nanofiltration, the other macromolecular components of such as protein in milk are retained by ultra-filtration and separation first, the solution of transmission is to contain lactose, inorganic salts, the aqueous solution of small molecule cellulose, then inorganic salts are recycled by electrodialytic technique, it obtains containing lactose, the desalination solution of small molecule cellulose and a small amount of inorganic salts, the desalination solution is after journey nanofiltration separation to retain lactose, to which lactose fraction be removed, finally by ultra-filter retentate and the merger and reorganization of electrodialysis concentrate and pass through spray drying, obtain demulsification sugar skimmed milk power.The present invention is by membrane separation technique with the aperture sieve departure section of physics, chemical affect will not be generated to milk, not only realize effectively removing and recycling for lactose, simultaneously during production, waste water has also obtained effective recycling, process is not in any by-product, and the rejection of lactose is up to 96.13%.
Description
Technical field
The present invention relates to a kind of demulsification sugar skimmed milk power preparation methods based on separation membrane technology.
Background technique
Calcium rich in, vitamin D etc. in milk, including whole amino acid needed for growth in humans's development, digestibility
Up to 98%, wherein a variety of anticancer factors such as calcium, vitamin, lactoferrin and conjugated linoleic acid, there is anticancer, anti-cancer；
And vitamin A, can prevent dry skin and it is obscure, make whitening skin, it is glossy etc..However the presence of lactose intolerance makes
This some patients can not drink common milk, thus demulsification sugar-milk occur.But it is single in this complicated system of milk
Singly abjection lactose is very difficult, and widely applied demulsification sugar method is enzymatic hydrolysis lactose now, however the cost of enzyme is high
The disadvantages of expensive and free lactase difficulty recycling, enzyme easy in inactivation.
Summary of the invention
For above-mentioned technical problem of the existing technology, the purpose of the present invention is to provide one kind based on separation membrane technology
Demulsification sugar skimmed milk power preparation method.
A kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology, it is characterised in that including following step
It is rapid:
1) skim milk by ultrafiltration membrane carry out dialysis for several times retain protein, obtain containing protein, dietary fiber first
Trapped fluid and the first permeate containing lactose, inorganic salts, small molecule cellulose；
2) the first permeate obtained by step 1) carries out desalting processing by electrodialysis, obtain containing be enriched with the concentrates of inorganic salts with
Desalination solution containing lactose, small molecule cellulose, the desalination solution is interior not to remove complete inorganic salts containing a small amount of；
3) the separating again by nanofiltration membrane progress inorganic salts and lactose of desalination solution obtained by step 2, obtains containing lactose, small point
Second trapped fluid of subbundle element and the second permeate containing a small amount of inorganic salts, second permeate are re-circulated to step
2) in electrodialytic process, inorganic salts are recycled；
4) merge concentrate obtained by the first trapped fluid obtained by step 1) and step 2 and obtain demulsification sugar skim milk, the demulsification sugar
Skim milk obtains demulsification sugar skimmed milk power by spray drying.
A kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology, it is characterised in that in step 1),
Skim milk is 6 times by the number that ultrafiltration membrane carries out dialysis, detailed process are as follows: skim milk carries out 6 infiltrations by ultrafiltration membrane
When analysis, the material liquid that each dialysis uses is diluted with 1 ~ 10 times of deionized water respectively, then is carried out by ultrafiltration membrane next
Secondary dialysis, wherein the material liquid that the 1st dialysis uses is the skim milk, on the material liquid that the 2nd ~ 6 dialysis uses is
The trapped fluid that dialysis obtains；Merge the permeate that 1 ~ 6 dialysis obtains and contains lactose, inorganic salts, small point to get to described
First permeate of subbundle element；The trapped fluid obtained after 6th dialysis be it is described containing protein, dietary fiber first
Trapped fluid.
A kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology, it is characterised in that in step 1)
Ultrafiltration membrane is plate membrane, ceramic membrane, hollow-fibre membrane or rolled membrane module.
A kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology, it is characterised in that step 2 electric osmose
Film used by analysing is heterogeneous, homogeneous or half homogeneous amberplex.
A kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology, it is characterised in that in step 3)
Nanofiltration membrane is plate membrane or rolled membrane module.
Compared with the existing technology, the beneficial effect that the present invention obtains is:
The present invention proposes that one kind using skim milk as raw material, is taken off based on coupling ultrafiltration, electrodialysis, nanofiltration these three membrane technologies
The preparation method of lactose defatted milk powder, the present invention retain the other macromolecular groups of such as protein in milk by ultra-filtration and separation first
Point, inorganic salts are then recycled by electrodialytic technique, then lactose is retained by nanofiltration separation, to lactose fraction be removed, most
At last ultra-filter retentate and the merger and reorganization of electrodialysis concentrate and by spray drying, obtain demulsification sugar skimmed milk power.The present invention
By membrane separation technique with the aperture sieve departure section of physics, any chemical affect will not be generated to milk, not only realizes cream
Sugar is effectively removed and is recycled, while during production, and waste water has also obtained effective recycling, and process is not in appoint
What by-product.By means of the present invention, the rejection of lactose is up to 96.13%, i.e., the mass content of lactose is down to 0.16%(g/
100g).
Specific embodiment
The present invention is further explained in the light of specific embodiments, but the scope of protection of the present invention is not limited thereto.
In following embodiment, the fat-free milk starting material of use is available from the degreasing ox that Zhejiang beauty is good for dairy industry Co., Ltd
Milk.The testing standard of lactose concn is GB/T5413.5-2010.
Protein concentration uses Coomassie brilliant blue test method, test process are as follows: by the sample liquid containing protein and examines horse
After the mixing of this brilliant blue reagent, mixed liquor tests light absorption value by spectrophotometer, and then tests out content (this method of protein
Test the total content of all proteins).
Embodiment 1
Step 1: by 1000g skim milk be 100KDa with molecular cut off PES plate membrane in 0.1MPa pressure and 6L/min
6 dialysis retention protein is carried out under flow velocity, the material liquid of each dialysis is diluted with 1L deionized water, then passes through ultrafiltration
Film carries out dialysis next time, and (in the present embodiment, the material liquid that the 1st dialysis uses for the skim milk, adopt by the 2nd ~ 6 dialysis
Material liquid is the trapped fluid that last dialysis obtains；When carrying out preceding 5 dialysis, the trapped fluid that each dialysis obtains is about
For 1000g)；Merge the obtained permeate of 1 ~ 6 dialysis to get to containing lactose, inorganic salts, small molecule cellulose first thoroughly
Cross liquid 6000g；Collect the trapped fluid 989.5g obtained after the 6th dialysis, as the first retention containing protein, dietary fiber
Liquid.
Step 2: the first permeate of step 1 gained 6000g is subjected to desalting processing (operating condition are as follows: the by electrodialysis
One permeate is added in the diluting compartment of electrodialysis plant, and 500g pure water, electric osmose are added in the enriched chamber of electrodialysis plant
Analysis carries out under 30V voltage and 40L/h flow velocity), obtain the concentrate 797.6g containing most of inorganic salts and containing remaining
The desalination solution 5700.3g of lactose, small molecule cellulose and fraction inorganic salts；
Step 3: the 5700.3g desalination solution that step 2 obtains being subjected to inorganic salts by PA nanofiltration membrane and (is grasped with separating again for lactose
Make condition are as follows: pressure 10bar, flow velocity 7L/min, 25 ± 5 DEG C of temperature), it obtains containing lactose, second section of small molecule cellulose
Stay liquid (i.e. lactose concentrate detects in lactose concentrate substantially without protein residues) 873.8g and containing a small amount of inorganic salts the
Two permeate (i.e. inorganic salt solution) 4824.7g, the third permeate can be re-circulated to the electrodialytic process of step 2
In, inorganic salts are recycled；
Step 4: by step 1 the first trapped fluid containing the relatively large substance such as protein, dietary fiber, in step 2
Concentrate containing most of inorganic salts merges, and obtains demulsification sugar-milk 1787.1g, and the demulsification sugar-milk is passed through
XINW-015 experiment type spray drier is spray-dried, and the demulsification sugar skimmed milk power that lactose mass content is 0.16% is obtained
23.5g。
Embodiment 2
Step 1: by 2000g skim milk be 100KDa with molecular cut off PES plate membrane in 0.1MPa pressure and 6L/min
6 dialysis retention protein is carried out under flow velocity, the material liquid of each dialysis is diluted with 2L deionized water, then passes through ultrafiltration
Film carries out dialysis next time, and (in the present embodiment, the material liquid that the 1st dialysis uses for the skim milk, adopt by the 2nd ~ 6 dialysis
Material liquid is the trapped fluid that last dialysis obtains；When carrying out preceding 5 dialysis, the trapped fluid that each dialysis obtains is about
For 2000g)；Merge the obtained permeate of 1 ~ 6 dialysis to get to containing lactose, inorganic salts, small molecule cellulose first thoroughly
Cross liquid 12000g；The trapped fluid 1989.5g obtained after the 6th dialysis is collected, as containing protein, first section of dietary fiber
Stay liquid.
Step 2: by the first permeate of step 1 gained 12000g by electrodialysis carry out desalting processing (operating condition are as follows:
First permeate is added in the diluting compartment of electrodialysis plant, and 500g pure water is added in the enriched chamber of electrodialysis plant, electricity
Dialysis carries out under 30V voltage and 40L/h flow velocity), obtain the concentrate 798.3g containing most of inorganic salts and containing remaining
Lactose, small molecule cellulose and fraction inorganic salts desalination solution 11697.7g；
Step 3: 11697.7g desalination solution that step 2 obtains is subjected to separating again for inorganic salts and lactose by PA nanofiltration membrane
(operating condition are as follows: pressure 10bar, flow velocity 7L/min, 25 ± 5 DEG C of temperature), obtain containing lactose, small molecule cellulose second
Trapped fluid (i.e. lactose concentrate detects in lactose concentrate substantially without protein residues) and contains a small amount of inorganic salts at 879.3g
Second permeate (i.e. inorganic salt solution) 10815.6g, the third permeate can be re-circulated to the electrodialysis of step 2
Cheng Zhong recycles inorganic salts；
Step 4: by step 1 the first trapped fluid containing the relatively large substance such as protein, dietary fiber, in step 2
Concentrate containing most of inorganic salts merges, and obtains demulsification sugar-milk 2787.8g, and the demulsification sugar-milk is passed through
XINW-015 experiment type spray drier is spray-dried, and the demulsification sugar skimmed milk power that lactose mass content is 0.17% is obtained
46.2g。
Embodiment 3
Step 1: by 3000g skim milk be 100KDa with molecular cut off PES plate membrane in 0.1MPa pressure and 6L/min
6 dialysis retention protein is carried out under flow velocity, the material liquid of each dialysis is diluted with 3L deionized water, then passes through ultrafiltration
Film carries out dialysis next time, and (in the present embodiment, the material liquid that the 1st dialysis uses for the skim milk, adopt by the 2nd ~ 6 dialysis
Material liquid is the trapped fluid that last dialysis obtains；When carrying out preceding 5 dialysis, the trapped fluid that each dialysis obtains is about
For 3000g)；Merge the obtained permeate of 1 ~ 6 dialysis to get to containing lactose, inorganic salts, small molecule cellulose first thoroughly
Cross liquid 18000g；The trapped fluid 2993.1g obtained after the 6th dialysis is collected, as containing protein, first section of dietary fiber
Stay liquid.
Step 2: by the first permeate of step 1 gained 18000g by electrodialysis carry out desalting processing (operating condition are as follows:
First permeate is added in the diluting compartment of electrodialysis plant, and 1000g pure water is added in the enriched chamber of electrodialysis plant, electricity
Dialysis carries out under 30V voltage and 40L/h flow velocity), obtain the concentrate 1797.8g containing most of inorganic salts and containing remaining
Lactose, small molecule cellulose and fraction inorganic salts desalination solution 17196.4g；
Step 3: 17196.4g desalination solution that step 2 obtains is subjected to separating again for inorganic salts and lactose by PA nanofiltration membrane
(operating condition are as follows: pressure 10bar, flow velocity 7L/min, 25 ± 5 DEG C of temperature), obtain containing lactose, small molecule cellulose second
Trapped fluid (i.e. lactose concentrate detects in lactose concentrate substantially without protein residues) and contains a small amount of inorganic salts at 869.7g
Second permeate (i.e. inorganic salt solution) 16322.7g, the third permeate can be re-circulated to the electrodialysis of step 2
Cheng Zhong recycles inorganic salts；
Step 4: by step 1 the first trapped fluid containing the relatively large substance such as protein, dietary fiber, in step 2
Concentrate containing most of inorganic salts merges, and obtains demulsification sugar-milk 4790.9g, and the demulsification sugar-milk is passed through
XINW-015 experiment type spray drier is spray-dried, and the demulsification sugar skimmed milk power that lactose mass content is 0.17% is obtained
69.8g。
Claims (5)
1. a kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology, it is characterised in that the following steps are included:
1) skim milk by ultrafiltration membrane carry out dialysis for several times retain protein, obtain containing protein, dietary fiber first
Trapped fluid and the first permeate containing lactose, inorganic salts, small molecule cellulose；
2) the first permeate obtained by step 1) carries out desalting processing by electrodialysis, obtain containing be enriched with the concentrates of inorganic salts with
Desalination solution containing lactose, small molecule cellulose, the desalination solution is interior not to remove complete inorganic salts containing a small amount of；
3) the separating again by nanofiltration membrane progress inorganic salts and lactose of desalination solution obtained by step 2, obtains containing lactose, small point
Second trapped fluid of subbundle element and the second permeate containing a small amount of inorganic salts, second permeate are re-circulated to step
2) in electrodialytic process, inorganic salts are recycled；
4) merge concentrate obtained by the first trapped fluid obtained by step 1) and step 2 and obtain demulsification sugar skim milk, the demulsification sugar
Skim milk obtains demulsification sugar skimmed milk power by spray drying.
2. a kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology according to claim 1, feature exist
In step 1), skim milk is 6 times by the number that ultrafiltration membrane carries out dialysis, detailed process are as follows: skim milk passes through ultrafiltration
When film carries out 6 dialysis, the material liquid that each dialysis uses is diluted with 1 ~ 10 times of deionized water respectively, then passes through ultrafiltration
Film carries out dialysis next time, wherein the material liquid that the 1st dialysis uses is the skim milk, the original of the 2nd ~ 6 dialysis use
Feed liquid is the trapped fluid that last dialysis obtains；Merge the obtained permeate of 1 ~ 6 dialysis to get to it is described containing lactose,
First permeate of inorganic salts, small molecule cellulose；The trapped fluid obtained after 6th dialysis contains protein, meals described in being
Eat the first trapped fluid of fiber.
3. a kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology according to claim 1, feature exist
Ultrafiltration membrane in step 1) is plate membrane, ceramic membrane, hollow-fibre membrane or rolled membrane module.
4. a kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology according to claim 1, feature exist
The film used by step 2 electrodialysis is heterogeneous, homogeneous or half homogeneous amberplex.
5. a kind of demulsification sugar skimmed milk power preparation method based on separation membrane technology according to claim 1, feature exist
Nanofiltration membrane in step 3) is plate membrane or rolled membrane module.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114343020A (en) * | 2021-12-14 | 2022-04-15 | 南京工业大学 | Process for removing lactose from milk |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1757289A (en) * | 2005-09-19 | 2006-04-12 | 王永强 | Delactose dairy product and its preparing method |
| CN106417667A (en) * | 2016-08-24 | 2017-02-22 | 厦门世达膜科技有限公司 | Production method of desalted sheep whey powder |
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- 2019-01-22 CN CN201910058850.XA patent/CN109769950A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1757289A (en) * | 2005-09-19 | 2006-04-12 | 王永强 | Delactose dairy product and its preparing method |
| CN106417667A (en) * | 2016-08-24 | 2017-02-22 | 厦门世达膜科技有限公司 | Production method of desalted sheep whey powder |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114343020A (en) * | 2021-12-14 | 2022-04-15 | 南京工业大学 | Process for removing lactose from milk |
| CN114343020B (en) * | 2021-12-14 | 2023-10-24 | 南京工业大学 | Process for removing lactose from milk |
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