CN109758611A - A kind of solution spraying preparation method of active biological tissue engineering scaffold - Google Patents
A kind of solution spraying preparation method of active biological tissue engineering scaffold Download PDFInfo
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- CN109758611A CN109758611A CN201811654045.5A CN201811654045A CN109758611A CN 109758611 A CN109758611 A CN 109758611A CN 201811654045 A CN201811654045 A CN 201811654045A CN 109758611 A CN109758611 A CN 109758611A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 238000005507 spraying Methods 0.000 title 1
- 238000009987 spinning Methods 0.000 claims abstract description 62
- 239000000243 solution Substances 0.000 claims abstract description 31
- 239000000835 fiber Substances 0.000 claims abstract description 29
- 239000007921 spray Substances 0.000 claims abstract description 28
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 21
- 102000018358 immunoglobulin Human genes 0.000 claims abstract description 21
- 238000002347 injection Methods 0.000 claims abstract description 21
- 239000007924 injection Substances 0.000 claims abstract description 21
- 230000000694 effects Effects 0.000 claims abstract description 17
- 239000007864 aqueous solution Substances 0.000 claims abstract description 13
- 239000003519 biomedical and dental material Substances 0.000 claims abstract description 12
- 230000005855 radiation Effects 0.000 claims description 22
- 239000001913 cellulose Substances 0.000 claims description 11
- 229920002678 cellulose Polymers 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 9
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 6
- 238000004132 cross linking Methods 0.000 claims description 6
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- 239000011858 nanopowder Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 229920001992 poloxamer 407 Polymers 0.000 claims description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 229920001661 Chitosan Polymers 0.000 claims description 2
- 229920001046 Nanocellulose Polymers 0.000 claims description 2
- 239000001506 calcium phosphate Substances 0.000 claims description 2
- 229910021389 graphene Inorganic materials 0.000 claims description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 2
- 229940078499 tricalcium phosphate Drugs 0.000 claims description 2
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 2
- 235000019731 tricalcium phosphate Nutrition 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 238000000338 in vitro Methods 0.000 abstract description 3
- 230000029663 wound healing Effects 0.000 abstract description 3
- 238000002513 implantation Methods 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 35
- 210000004027 cell Anatomy 0.000 description 34
- 210000000056 organ Anatomy 0.000 description 9
- 238000000034 method Methods 0.000 description 7
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- 125000003367 polycyclic group Chemical group 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 2
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
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- 229940126864 fibroblast growth factor Drugs 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
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- 239000012528 membrane Substances 0.000 description 2
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- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 102000056172 Transforming growth factor beta-3 Human genes 0.000 description 1
- 108090000097 Transforming growth factor beta-3 Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
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- 238000001523 electrospinning Methods 0.000 description 1
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- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 210000002966 serum Anatomy 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/06—Wet spinning methods
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/12—Stretch-spinning methods
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Textile Engineering (AREA)
- Mechanical Engineering (AREA)
- Dispersion Chemistry (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Botany (AREA)
- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The technical program discloses a kind of molten spray Preparation Method of active bio tissue engineering bracket, which comprises the following steps: (1) dissolves bio-medical material, obtain the bio-medical material aqueous solution that concentration is 2~50%；(2) immunoglobulin solution is added in bio-medical material aqueous solution obtained in step (1), activity spinning solution is made；(3) the pressure-air injection stream for being 0~37 DEG C using temperature carries out molten spray spinning as pulling motion, and receives spinning using spinning receiver；(4) it is equipped with cell printing device after fiber receiver, the living solution containing seed cell or Porcine HGF is printed in step (3) and is made in tunica fibrosa, finally obtained active bio tissue engineering bracket.Active bio tissue engineering bracket obtained can make bioengineered tissue bracket by implantation used inside human body after carrying out appropriate in vitro culture in the technical program, or as skin tissue engineering scaffold, promote wound healing.
Description
Technical field
The invention belongs to biomedical material technology, in particular to a kind of molten spray of bioengineered tissue bracket is standby
Method.
Background technique
The missing or functional failure of tissue and organ, which constitute health and lives, to be seriously threatened.The mankind for a long time
Constantly exploring improves own health level with research and utilization material and biotechnology.1993, Massachusetts Institute Technology
Chemical Engineer Langer R and the doctor Vacanti JP of medical college, Harvard University systematically propose organizational project thought, i.e.,
On timbering material plant human body living cells, organization of production under the action of growth factor, and propose organizational project this without exception
It reads.
The basic principle and method of organizational project are to plant the self or variant cell of amplification in vitro in external structure
In extracellular matrix analogies (bracket), cell/scaffold complex is formed.Then by the cell-scaffold compound implant damage
Tissue or organ sites, the proliferation and differentiation and the degradation that matches of class extracellular matrix bracket for passing through implantation cell absorb
And new tissue or organ that formation mechanism is consistent with destination organization or organ with function, to reach wound repair and function weight
The purpose built.
The research contents of organizational project be concentrated mainly on the cultivation of seed cell, the development of tissue engineering bracket bracket and
The engineered tissue of cell/scaffold complex component or organ etc..Wherein tissue engineering bracket is cell and tissue life
It is long that suitable environment is provided, it gradually degrades and disappears with the division of cell, so that new space is supplied to tissue and thin
Born of the same parents, and newly-generated tissue and organ is made to have geometric shape identical with cytoskeleton.Bracket is engineered in component
Main function in tissue or organ includes: that (1) for the adherency of cell provides physical support, and cell is accurately delivered to
Damaged part；It (2) is the increasing of cell, metabolism provides space；(3) specific macroscopic view and microstructure are provided, cell component is guided
The tissue or organ of specific function；(4) transmitting chemistry or mechanical signal, the phenotype of regulating cell.The design of tissue engineering bracket
Be involved in the problems, such as three scales with building, respectively macroscopical (centimetre scale above), i.e. shape, microscopic aperture, porosity and
Rack surface topological structure (micron order scale), influence (the nanoscale ruler of rack surface attachment proteins and gene pairs cell
Degree).Ideal tissue engineering bracket has basic demand below: (1) good cell compatibility；Except meeting the one of biomaterial
As require as it is nontoxic, teratogenesis, catabolite to cytotoxic evil effect, do not cause except inflammatory reaction, to be also conducive to plant
The adherency of daughter cell, proliferation, it is often more important that the special gene expression of energy active cell maintains the phenotypic expression of cell；(2) good
Good biological degradability；Timbering material should be able to degrade after repair of damaged tissues, and degradation speed should be with the speed phase of regeneration
Matching；(3) there is 3 D stereo porous structure, there is aperture appropriate, high porosity and big specific surface area；(4) appropriate
Mechanical strength, bracket should have the mechanical strength to match with institute repair tissue, provide support for cambium, can maintain group
Knit original form of organ；(5) it infects in order to prevent, bracket must also be easy to sterilize and save.
Nanofiber be widely used in due to high-specific surface area and porous structure drug controlled release, artificial skin,
The medical domains such as wound healing and tooth enhancing, but the tunica fibrosa for tissue engineering bracket at present on the market is all excessively quiet
Electrospinning preparation.The basic principle of electrostatic spinning are as follows: by high-pressure electrostatic on precursor solution band, the polymerization thing liquid of electrification
Drop is accelerated in the Taylor conical point of capillary under the active force of electric field；When electric field force is sufficiently large, polymer drop can
Surface tension is overcome to form injection thread；Thread solvent volatilization or solidification in course of injection, finally fall on receiving device, shape
At the fibrofelt of similar non-woven cloth-like.It can be seen that electrostatic spinning needs high-pressure electrostatic from the principle of electrostatic spinning, therefore
When preparing biologically active tunica fibrosa, high-pressure electrostatic certainly will will affect it and add active material in spinning solution
Bioactivity, to reduce the bioactivity of the active bio tissue engineering bracket of preparation, and electrostatic spinning technique prepares nothing
Spinning fiber film needs high voltage, defective in terms of operational safety, it is not easy to large-scale application in the industrial production, electrostatic
It spins production technology and is in and just enter the industrial production stage, need to overcome there are also much difficult.
The production of non-woven fibre membranous tissue engineering rack at present is mostly to prepare mutually to separate with cell culture, is be easy to cause
Growth of the cell in rack surface.
Summary of the invention
The present invention provides a kind of molten spray Preparation Method of active bio tissue engineering bracket, and the preparation method can imitate certainly
The bioengineered tissue bracket of the animal spinning process on right boundary, practical the method preparation does not allow the life for easily causing cell on its surface
It is long.
To achieve the above object, the technical program uses following technological means.
A kind of molten spray Preparation Method of active bio tissue engineering bracket, comprising the following steps:
(1) bio-medical material is dissolved, obtains the bio-medical material aqueous solution that concentration is 2~50%；
(2) immunoglobulin solution is added in bio-medical material aqueous solution obtained in step (1), activity is made and spins
Silk liquid, wherein the concentration of immunoglobulin is 1~10% in immunoglobulin solution；
(3) the pressure-air injection stream for being 0~37 DEG C using temperature carries out molten spray spinning as pulling motion, and uses spinning
Receiver receives spinning, and fibrous framework is made；
(4) cell printing device is equipped with after fiber receiver, by the work containing seed cell or Porcine HGF
Property solution print to and be made in step (3) in fibrous framework, final obtained active bio tissue engineering bracket.
A kind of molten spray Preparation Method of active bio tissue engineering bracket further includes following step: obtained to step (2)
Inorganic nanometer powder or organic nano powder is added after mixing in active spinning solution, as activity used in step (3)
Spinning solution.
Further, the inorganic nanometer powder is one of hydroxyapatite, tricalcium phosphate, graphene.
Further, the organic nano powder is nanocrystalline cellulose.
Further, the bio-medical material is chitosan, sodium alginate, polyvinyl alcohol, bacteria cellulose, polycyclic oxygen
One of ethylene, cellulose and polyethylene glycol.
Further, pressure-air injection stream described in step (3) is by dry compressed air, relative humidity
It is 10%~100%.
Further, above the spinning receiver in step (3) be equipped with radiation appliance, the radiation appliance to spinning into
Row crosslinking with radiation；Crosslinking with radiation is carried out to spinning using radiation appliance, wherein being 1800mW/cm to the radiation intensity of spinning2, this
The degree of cross linking between large biological molecule can be improved in fiber in sample, reduces gained spinning fibre in the solubility of aqueous solution, to improve
The intensity of spinning fibre.
Further, the radiation source of the radiation appliance is ionized radiation source, including x-ray radiation source, β particle spoke
Penetrate one of source, alpha-radiation source, λ ray radiation source.
Further, when the spinning receiver receives plate using spinning, it is fine to obtain active bio organizational project
Tie up film.
Further, the spinning receiver using spinning receive roller when, obtain spinning fibre simple tension and
The active bio organizational project continuous fiber film of one direction ordered arrangement.
Further, the injection pressure in pressure-air injection stream used is 10MPa~30MPa.
The technical program has the beneficial effect that preparation method described in the technical program does not need voltage, and with water
For solvent, it is easier to large-scale application in the industrial production；The biology prepared by preparation method described in the technical program
Tissue engineering bracket structure is similar to electrostatic spinning fiber film, it may have high porosity, and the preparation method can facilitate tune
Machined parameters are saved, requirement of the cell growth to material porosity is met, the structure as made of nanofiber layer upon layer can ensure that
The hole link that the bioengineered tissue bracket of preparation has had；The bioengineered tissue bracket prepared using the technical program
Fibre diameter be 100nm~10 μm, therefore can farthest imitate human body cell epimatrix structure；Using the preparation
The bioengineered tissue bracket of method preparation has big specific surface area, can provide good microenvironment for the existence of cell, have
Sticking, break up and being proliferated conducive to cell；The activated fibre non-woven fabrics for being implanted with seed cell is made in this method, can carry out appropriateness
It is implanted into used inside human body after in vitro culture and makees bioengineered tissue bracket, or as skin tissue engineering scaffold, promotes wound healing；
Immunoglobulin solution is added in the application in traditional spinning solution, immunoglobulin (Ig) refers to have
Antibody activity or chemical structure, globulin similar with antibody molecule；Immunoglobulin is divided into antibody and membrane immunoglobulin, resists
Body is primarily present in serum, and major function is specifically to combine antigen；Membrane immunoglobulin be antigen on B cell film by
Body, can specific recognition antigen molecule；Immunoglobulin energy activating complement, therefore use the spinning solution for being added to immunoglobulin
Active bio tissue engineering bracket obtained is carried out compared with traditional tunica fibrosa, active bio tissue engineering bracket is more advantageous to
The adherency of seed cell, proliferation, and the gene expression that Porcine HGF energy active cell is special, maintain the phenotype table of cell
It reaches；
But the activity of immunoglobulin is by temperature, the influence of the factors such as solution ph, and with temperature in the technical program
It is pulling motion for 0~37 DEG C of pressure-air injection stream, carries out molten spray spinning, immunoglobulin is 0~37 in temperature range
At DEG C, activity is simultaneously unaffected；And after temperature is higher than 37 DEG C, since temperature is excessively high, then it will lead to immunoglobulin and lose
Activity, therefore the technical program uses and carries out activity and fibre that molten spray spinning then effectively maintains immunoglobulin at 0~37 DEG C
Tie up the structural intergrity of spinning.
Specific embodiment
Technical solution of the present invention is further illustrated combined with specific embodiments below.It should be understood that these embodiments are only used for
Illustrate the present invention rather than limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, this
Field technical staff can make various changes or modifications the present invention, and such equivalent forms equally fall within right appended by the application and want
Seek book limited range.
Embodiment 1
A kind of molten spray Preparation Method of active bio engineering rack, comprising the following steps: (1) with NaOH (7wt%)/urea
(12wt%) aqueous solution is that solvent dissolves cellulose at -12 DEG C, obtains the cellulose solution of 5wt%；(2) by nanometer crystal fiber
Element is added in cellulose solution, is stirred evenly, and cellulose spinning fluid is made；(3) to cellulose spinning fluid made from step (2)
The middle immunoglobulin solution that concentration is added and is 10%, is made activity spinning solution；(4) with 37 DEG C of temperature, relative humidity 100%
Pressure-air injection stream be pulling motion, the injection pressure in pressure-air injection stream used is 30MPa, by active spinning solution
Molten spray spinning is carried out, spinning receiver used is that spinning receives plate, obtains cellulose/nanometer crystalline cellulose active bio tissue
Engineering rack, wherein cell printing liquid be containing concentration be 5% fibroblast growth factor and concentration be 20% skin
Fibroblastic bovine serum albumin aqueous solution；Active bio tissue engineering bracket described in the technical program is finally obtained, and
Average fibre diameter is 2 μm.
Embodiment 2
A kind of molten spray Preparation Method of active bio engineering rack, comprising the following steps: (1) take water as a solvent dissolution polycyclic
Ethylene oxide (relative molecular weight 1,000,000), obtains 10wt% Pluronic F-127 aqueous solution；(2) to polycyclic oxygen second made from step (1)
The immunoglobulin solution that concentration is 10% is added in alkene spinning solution, activity spinning solution is made；(3) with 37 DEG C of temperature, humidity is
The pressure-air injection stream of 25wt% is pulling motion, and the injection pressure in pressure-air injection stream used is 25MPa, by polycyclic
Ethylene oxide spinning solution carries out molten spray spinning, and spinning receiver used is that spinning receives plate, obtains fibrous framework；Spinning receives flat
It is equipped with λ ray radiation apparatus above plate, crosslinking with radiation is carried out to fiber using the λ ray radiation apparatus, wherein radiation intensity
For 1800mW/cm2, the solubility of Pluronic F-127 fiber in aqueous solution is reduced, fiber mechanics intensity, cell printing dress are improved
Setting middle cell printing liquid is the bone shape containing the articular chondrocytes that concentration is 10% transforming growth factor β 3 and concentration is 30%
Protein solution occurs for state, finally obtains active bio tissue engineering bracket described in the technical program, and fiber is average straight
Diameter is 500nm.
Embodiment 3
A kind of molten spray Preparation Method of active bio engineering rack, comprising the following steps: (1) take water as a solvent the poly- second of dissolution
Enol (relative molecular weight 100,000), obtains 50wt% polyvinyl alcohol water solution；(2) to polyvinyl alcohol spinning made from step (1)
The immunoglobulin solution that concentration is 15% is added in liquid, activity spinning solution is made；(3) with 37 DEG C of temperature, humidity 10wt%
Pressure-air injection stream be pulling motion, the injection pressure in pressure-air injection stream used is 20MPa, and polyvinyl alcohol is spun
Silk liquid carries out molten spray spinning, and spinning receiver used is that spinning receives plate, obtains molten blown nonwoven fiber film, the spinning receives
It is equipped with λ ray radiation apparatus above plate, and crosslinking with radiation is carried out to fiber using the λ ray radiation apparatus, wherein radiating
Intensity is 1800mW/cm2, the solubility of the molten spray fiber of polyvinyl alcohol in aqueous solution is reduced, fiber mechanics intensity, cell are improved
In printing equipment cell printing liquid be containing concentration be 1% fibroblast growth factor and concentration be 30% skin at fibre
The bovine serum albumin aqueous solution of cell, non-woven fibre film described in final the technical program are tieed up, and average fibre diameter is
200nm。
The better embodiment of the technical program is illustrated above, but the technical program be not limited to it is described
Embodiment, those skilled in the art can also make various equivalent changes under the premise of without prejudice to the technical program spirit
Type or replacement, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (10)
1. a kind of molten spray Preparation Method of active bio tissue engineering bracket, which comprises the following steps:
(1) bio-medical material is dissolved, obtains the bio-medical material aqueous solution that concentration is 2~50%；
(2) immunoglobulin solution is added in bio-medical material aqueous solution obtained in step (1), activity spinning is made
Liquid, wherein the concentration of immunoglobulin is 1~15% in immunoglobulin solution；
(3) the pressure-air injection stream for being 0~37 DEG C using temperature carries out molten spray spinning as pulling motion, and is received using spinning
Device receives spinning, and fibrous framework is made；
(4) cell printing device is equipped with after fiber receiver, the activity containing seed cell or Porcine HGF is molten
Liquid is printed in step (3) and is made in fibrous framework, finally obtained active bio tissue engineering bracket.
2. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that also
Include the following steps: that inorganic nanometer powder is added in the activity spinning solution made from step (2) or the mixing of organic nano powder is equal
After even, as activity spinning solution used in step (3).
3. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 2, which is characterized in that institute
Stating inorganic nanometer powder is one of hydroxyapatite, tricalcium phosphate, graphene.
4. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 2, which is characterized in that institute
Stating organic nano powder is nanocrystalline cellulose.
5. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that institute
Stating bio-medical material is chitosan, sodium alginate, polyvinyl alcohol, bacteria cellulose, cellulose, Pluronic F-127 and poly- second two
One of alcohol.
6. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that step
Suddenly pressure-air injection stream described in (3) is by dry compressed air, and relative humidity is 10%~100%.
7. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that
Radiation appliance is equipped with above spinning receiver in step (3), the radiation appliance carries out crosslinking with radiation to spinning, wherein to spinning
The radiation intensity of silk is 1800mW/cm2。
8. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that institute
When stating spinning receiver using spinning reception plate, active bio tissue-engineering fiber film is obtained.
9. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that institute
When stating spinning receiver using spinning reception roller, the activity of spinning fibre simple tension and one direction ordered arrangement is obtained
Bioengineered tissue continuous fiber film.
10. a kind of molten spray Preparation Method of active bio tissue engineering bracket according to claim 1, which is characterized in that
Injection pressure in pressure-air injection stream used is 10MPa~30MPa.
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| PCT/CN2019/113151 WO2020134445A1 (en) | 2018-12-28 | 2019-10-25 | Solution spray preparation method for scaffold for active biological tissue engineering |
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| CN110592686A (en) * | 2019-08-30 | 2019-12-20 | 河南亚都实业有限公司 | Growth factor loaded micro-nano composite membrane |
| WO2020134444A1 (en) * | 2018-12-28 | 2020-07-02 | 佛山科学技术学院 | Preparation method for biological tissue engineering stent by solution spraying |
| WO2020134445A1 (en) * | 2018-12-28 | 2020-07-02 | 佛山科学技术学院 | Solution spray preparation method for scaffold for active biological tissue engineering |
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| CN112717191B (en) * | 2021-01-08 | 2021-11-16 | 齐齐哈尔大学 | A kind of preparation method of customizable, functional dressing |
| CN114870070A (en) * | 2021-02-05 | 2022-08-09 | 诺一迈尔(苏州)生命科技有限公司 | An organic/inorganic composite three-dimensional porous nanofiber tissue engineering scaffold and its preparation method and application |
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