CN109744983B - Zoom type cavity endoscope detection device and laser scanning cavity endoscope - Google Patents
Zoom type cavity endoscope detection device and laser scanning cavity endoscope Download PDFInfo
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Abstract
The embodiment of the invention provides a zoom type cavity endoscope detection device and a laser scanning cavity endoscope. The zoom type cavity endoscope detection device comprises a handle shell and a detection tube, wherein the handle shell and the detection tube relatively move up and down through a first transmission ring and a second transmission ring, and a first light path comprising a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror, a relay mirror and an objective lens and a second light path comprising the objective lens, the relay mirror and the dichroic mirror are arranged in a middle through tube of the handle shell. According to the variable-focus cavity endoscope detection device and the laser scanning cavity endoscope, the detection tube and the handle shell are driven to move up and down relatively through the up-and-down movement between the driving rings, so that the optical path structure in the handle shell can perform zooming operation, cell imaging of different depths of gastrointestinal tissues, oral tissues, uterine cavity tissues and the like in the abdominal cavity of a human body is realized, and relevant conditions such as the infiltration depth of tumors, metastasis conditions, the existence of cancer residues at the cutting edge of surgical operation and the like are accurately judged.
Description
Technical Field
The embodiment of the invention relates to the technical field of laser scanning endoscopes, in particular to a zoom type cavity endoscope detection device and a laser scanning cavity endoscope.
Background
Gastrointestinal malignancy is the second leading cause of cancer death in the developed world population, and this trend has become more apparent in recent years. Surgical radical excision is mainly adopted for treating malignant tumor of gastrointestinal tract, but the specific range of the surgical excision needs to be determined when the surgical radical excision is carried out, so that the benign and malignant tumor, the infiltration depth, the metastasis condition, the existence of cancer residues at the incisional edge and the like need to be known before the operation is carried out. Therefore, preoperative gastrointestinal biopsy is an important diagnostic evidence for histological diagnosis of gastrointestinal tumors. And according to tumor size, growth position, infiltration depth, etc., gastric cancer is classified into gastric total incision, gastric sub-total incision, partial gastrectomy, endoscopic submucosal or submucosal resection, etc.
At present, the biopsy under the gastrointestinal endoscope is usually based on the gastrointestinal endoscope, and is carried out imaging by CT, MRI and the like as assistance, or is evaluated and stored by a traditional white-light laparoscope or endoscope.
However, imaging with the aid of CT, MRI, etc. on the basis of a gastrointestinal endoscope has some unavoidable drawbacks, such as easy bleeding from the intestinal canal or tumor during operation, need for manual pulling or squeezing, delay of time due to repeated endoscopic biopsy when the gastrointestinal endoscope cannot pass through the intestinal canal, and need for additional emergency hemostasis if severe bleeding is caused, etc. However, the auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumor and the lymph node metastasis in clinical practice. The ultrasonic endoscope is used for judging the T stage of the gastrointestinal tumor, and the literature reports that the accuracy is only 44.7% -78%, which is insufficient to become a reliable diagnosis standard. The ultrasonic endoscope has poor preoperative evaluation effect on the partial excision operation, cannot accurately subdivide gastrointestinal mucosa layers, and has poor N-stage effect. Therefore, in view of the current auxiliary diagnosis technology of gastrointestinal tract, a new diagnosis device for gastrointestinal tract tumor is needed to detect the gastrointestinal tract tissue information of different depths in situ in real time.
Disclosure of Invention
Aiming at the technical problems in the prior art, the embodiment of the invention provides a zoom type cavity endoscope detection device and a laser scanning cavity endoscope.
In a first aspect, an embodiment of the present invention provides a zoom type endoscope probe device including:
Handle casing and probe tube, handle casing bottom is provided with well siphunculus and first transmission ring, the probe tube top is provided with the second transmission ring, first transmission ring with the second transmission ring rotates to be connected, well siphunculus nestification is in the probe tube with in the first transmission ring, be provided with the motor that zooms in the handle casing and be used for forming the light path structure of first light path and second light path, wherein:
the zoom motor is used for driving the second transmission ring to move up and down relative to the first transmission ring;
the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror, a relay mirror and an objective lens, wherein the first optical path is used for conducting laser signals received by the collimating lens from the collimating lens to the objective lens;
The second optical path sequentially comprises the objective lens, the relay lens and the dichroic mirror, wherein the second optical path is used for conducting optical signals collected by the objective lens from the objective lens to the dichroic mirror.
In a second aspect, embodiments of the present invention provide a three-dimensional nonlinear laser scanning cavity endoscope, comprising:
The device comprises a fluorescence collection device, a scanning acquisition controller, a femtosecond pulse laser, an optical fiber coupling module and the zoom type cavity endoscope detection device provided by the first aspect of the embodiment of the invention, wherein the fluorescence collection device and the optical fiber coupling module are both in optical fiber communication connection with the zoom type cavity endoscope detection device, and the fluorescence collection device and the zoom type cavity endoscope detection device are both electrically connected with the scanning acquisition controller, wherein:
The femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;
The optical fiber coupling module is used for coupling the pulse laser signals output by the femtosecond pulse laser and transmitting the pulse laser signals to the collimating lens in the zoom type cavity endoscope detection device;
The zoom type cavity endoscope detection device is used for receiving the pulse laser signals, outputting the pulse laser signals to autofluorescent substances in living cells, acquiring fluorescent signals and second harmonic signals generated after the autofluorescent substances are excited through the objective lens, and outputting the fluorescent signals and the second harmonic signals to the fluorescent collection device;
The fluorescence collection device is used for respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals after receiving the fluorescence signal and the second harmonic signal;
the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquiring the electric signals.
According to the zoom type cavity endoscope detection device provided by the embodiment of the invention, the second transmission ring of the detection tube is driven by the zoom motor to move up and down relative to the first transmission ring of the handle shell, so that the detection tube moves up and down relative to the handle shell, including an optical path structure arranged in the handle shell, and further an objective lens in the optical path structure can move up and down relative to the detection device to perform zooming operation, and tissue cell imaging with different depths can be performed when gastrointestinal tissues, oral tissues and intrauterine tissues in the abdominal cavity of a human body are detected, so that structural information with different depths of tissue cells can be obtained, and therefore, the infiltration depth, the transfer condition, the existence of cancer residues at the cutting edge of a surgical operation and other related conditions of the tumor can be judged more accurately, and the operation is simple and the use is convenient.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a zoom type endoscope detection device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram II of a zoom type endoscope detection device according to an embodiment of the present invention;
FIG. 3 is a schematic diagram III of a zoom type endoscope detection device according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a zoom type endoscope detection device according to an embodiment of the present invention;
FIG. 5 is a schematic view of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;
FIG. 6 is a schematic diagram II of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of a fluorescence collection device according to an embodiment of the present invention;
FIG. 8 is a schematic diagram of a box-type combined structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;
FIG. 9 is a second schematic diagram of a box-type combined structure of a three-dimensional nonlinear laser scanning cavity endoscope according to an embodiment of the present invention;
FIG. 10 is a schematic view of a desktop structure of an endoscope with a three-dimensional nonlinear laser scanning cavity according to an embodiment of the present invention;
FIG. 11 is a schematic diagram of a desktop structure of a three-dimensional nonlinear laser scanning cavity endoscope.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
At present, imaging is carried out based on a gastroenteroscope by taking CT, MRI and the like as assistance to obtain relevant information such as benign and malignant tumor, infiltration depth, metastasis condition, existence of cancer residues at a cutting edge and the like, and the imaging method has the defects in specific operation, such as easy bleeding of an intestinal canal or a tumor body, need of manual traction or extrusion, delay caused by repeated endoscopic biopsy when the gastroenteroscope cannot pass through the intestinal canal, and need of additional first aid hemostasis if severe bleeding is caused. However, the auxiliary examination means such as CT and MRI cannot accurately judge the infiltration depth of early gastrointestinal tumor and the lymph node metastasis in clinical practice. The ultrasonic endoscope is used for judging the T stage of the gastrointestinal tumor, the literature reports that the accuracy is only 44.7% -78%, the ultrasonic endoscope is insufficient to become a reliable diagnosis standard, the ultrasonic endoscope has poor preoperative judgment effect on the partial excision operation, the gastrointestinal mucosa layer cannot be accurately subdivided, and the N stage effect is poor.
Whereas conventional white light laparoscopes and endoscopes are capable of assessing many gastrointestinal disorders, the technique is limited to detecting gross morphological changes. Although suspicious regions are easily found, these techniques are related to false positive rate, specificity, and the like, as compared to in vivo detection techniques. White light endoscopy is associated with extensive errors in microscopic change diagnosis, including the diagnosis of ulcerative colitis or Barrett's oesophagus and flat adenomatous dysplasia. Confocal endoscopes have received extensive attention in combination with laser technology, fluorescence detection technology, rapid scanning technology, and the like, because of their ability to detect mucosal changes at the microscopic level, and the potential for replacement of tissue biopsies, the imaging technology has high sensitivity and specificity. However, the confocal endoscopic imaging technology is still limited by imaging depth and fluorescent dye, and because the gastrointestinal sample has strong absorption and scattering to visible light, the imaging depth is only in a shallow surface layer, and a specific fluorescent dye developer is also required to be injected, the operation is too complicated, and the relevant information such as the infiltration depth of tumor, the metastasis condition, the existence of cancer residues at the surgical incision edge and the like can not be accurately obtained.
The two-photon microscopic imaging technology adopts a femtosecond pulse laser with longer wavelength as an excitation light source, has the characteristics of deep imaging depth, small photodamage, small photobleaching area, high fluorescence collection efficiency and the like, and has epoch-making significance in deep imaging of biological tissues. W.Denk et al, university of Conneler, 1990 developed the first two-photon fluorescence microscope in the world, using multiphoton microscopy imaging techniques based on nonlinear optics and femtosecond pulsed lasers. The technology can rapidly obtain the tissue structure and the cell morphology of the specimen in real time by utilizing the self-fluorescence generated by cells in living tissues and the second harmonic generated by collagen tissues. As early as 1986, the second harmonic was used in skin studies and in coronary microscopy imaging studies, confirming its feasibility to be used for the observation of biological tissues. MPM may also be an important tool in cancer research. The autofluorescence generated by the cells is derived from Nicotinamide Adenine Dinucleotide (NADH) and Flavin Adenine Dinucleotide (FAD) in the cells, the wavelength of NADH is 460nm, and the secondary oscillation harmonic wave of collagen is 370-390 nm, so a multiphoton microscope in the range of 780-940 nm is usually selected when observing tumor specimen tissues. MPM imaging is not only comparable to standard tumor histopathology, but also provides additional information on tumor neogenesis processes, as reflected in the metabolic levels of tumor tissue cells by measuring the ratio NADH/FAD.
Using multiphoton imaging techniques, multiphoton microscopes can provide real-time gastrointestinal tissue structure and cell morphology information. The multiphoton imaging technology has the characteristics of no need of externally marking tissues, extremely sensitivity to collagen, small photodamage to tissues, deep penetration depth and the like, and can be applied to optical biopsy of gastrointestinal tumors. There are no clinically available two-photon laparoscope, endoscope and cavity endoscope detection device based on two-photon imaging to detect gastrointestinal tissue information in situ in real time.
In order to detect gastrointestinal tissue information of different depths in situ in real time, an embodiment of the present invention provides a zoom type cavity endoscope detection device, and fig. 1 is a schematic structural diagram of the zoom type cavity endoscope detection device provided in the embodiment of the present invention, as shown in fig. 1, the zoom type cavity endoscope detection device includes:
Handle casing 11 and detection tube 12, handle casing 11 bottom is provided with well siphunculus 13 and first transmission ring 111, and detection tube 12 top is provided with second transmission ring 121, and first transmission ring 111 rotates with second transmission ring 121 to be connected, and well siphunculus 13 nestification is provided with zoom motor 119 and has the light path structure that is used for forming first light path and second light path in detection tube 12 and first transmission ring 111 in the handle casing 11, wherein:
A zoom motor 119 for driving the second drive ring 121 to move up and down with respect to the first drive ring 111;
the first optical path sequentially comprises a collimating lens 113, a micro-electromechanical scanning galvanometer 114, a lens 115, a dichroic mirror 116, a relay mirror 117 and an objective lens 118, wherein the first optical path is used for conducting laser signals received by the collimating lens 113 from the collimating lens 113 to the objective lens 118;
The second optical path sequentially includes an objective lens 118, a relay lens 117, and a dichroic mirror 116, where the second optical path is used to conduct the optical signal collected by the objective lens 118 from the objective lens 118 to the dichroic mirror 116.
Specifically, the zoom type cavity endoscope detection device provided by the embodiment of the invention comprises a handle shell 11 and a detection tube 12, wherein the interior of the handle shell 11 is provided with a cavity, the bottom end of the shell is provided with an opening, a middle through tube 13 is arranged at the opening, the interior of the handle shell 11 is provided with a light path structure forming a first light path and a second light path, the first light path comprises a collimating lens 113, a micro-electromechanical scanning galvanometer 114, a lens 115, a dichroic mirror 116, a relay mirror 117 and an objective lens 118, the laser signals used for exciting autofluorescent substances in gastrointestinal tissues or oral tissue cells of a human body are emitted onto the autofluorescent substances from the object lens 118 after passing through the collimating lens 113, the micro-electromechanical scanning galvanometer 114, the lens 115, the dichroic mirror 116, the relay mirror 117 and the objective lens 118 in the first light path, and after the two-photon signals and the second-harmonic signals are generated by exciting the autofluorescent substances, the two-photon signal and the second harmonic signal are collected through the objective lens 118 and are collected into the fluorescence collecting device through the relay lens 117 and the dichroic mirror 116 in the second optical path for obtaining detection information of gastrointestinal tissues or oral tissues to be detected so as to judge relevant information such as the infiltration depth of tumors, the metastasis condition, whether cancer residues exist at the cutting edge of surgical operation and the like, wherein a first driving ring 111 is arranged outside an opening at the bottom end of the handle shell 11, a middle through pipe 13 is nested in the first driving ring 111, a second driving ring 121 at the top end of the detection pipe 12 is connected with the first driving ring 111 in a relative rotation fit manner, a zoom motor 119 arranged in the handle shell 11 drives the second driving ring 121 to move up and down relative to the first driving ring 111 in a relative rotation manner and to vertically move up and down in a direct drive manner, thereby realizing the optical path structure of the detection pipe 12 relative to the handle shell 11, and the whole light path structure can move up and down relative to the channel opening of the detection channel in the detection tube 12 to realize tissue cell detection with different depths and acquire cell structure information with different depths.
The relay lens 117 is disposed inside the objective lens 118, and is used for conducting the laser signal for exciting the autofluorescent substance from the dichroic mirror 116 to the objective lens 118 in a long distance, and conducting the two-photon signal and the second harmonic signal collected by the objective lens 118 to the dichroic mirror 116, wherein the image plane of the laser signal objective lens 118 coincides with the focal plane of the relay lens 117, and the laser signal scanning area passing through the micro-electromechanical scanning mirror is conducted to the image plane of the objective lens 118 in a ratio of 1:1, wherein the relay lens 117 can be lengthened or shortened according to specific needs.
The dichroic mirror 116 may be set as a long-pass short-reflecting dichroic mirror 116 or a short-pass long-reflecting dichroic mirror 116 according to needs, that is, when the long-pass short-reflecting dichroic mirror 116 is set, a pulse laser signal for exciting an autofluorescent substance is transmitted, and a collected two-photon signal and a second harmonic signal are reflected, as shown in fig. 1, and in this case, the zoom type cavity endoscope detection device may be a laparoscope or a hysteroscope detection device; fig. 2 is a schematic diagram of a second structure of a zoom-type cavity endoscope detection device according to an embodiment of the present invention, as shown in fig. 2, when the dichroic mirror 116 is a short-pass long-reflecting dichroic mirror 116, a pulse laser signal for exciting an autofluorescent substance is reflected, a collected two-photon signal and a second harmonic signal are transmitted, the dichroic mirror 116 reflects the laser signal incident on the dichroic mirror 116 after passing through the collimating lens 113, the mems scanning galvanometer 114 and the lens 115, and passes through the relay lens 117 to the objective lens 118, and the two-photon signal and the second harmonic signal collected by the objective lens 118 are transmitted.
The zoom type cavity endoscope detection device provided by the embodiment of the invention drives the second transmission ring of the detection tube to move up and down relative to the first transmission ring of the handle shell through the zoom motor so as to realize that the detection tube moves up and down relative to the handle shell, including an optical path structure arranged in the handle shell, so that an objective lens in the optical path structure can move up and down relative to the detection device to perform zooming operation, and tissue cell imaging with different depths can be performed when gastrointestinal tissue, oral tissue and intrauterine tissue in a human body are detected, so that structural information with different depths of tissue cells can be obtained, and the relative conditions such as the infiltration depth of tumors, the metastasis condition, the existence of cancer residues at the cutting edge of surgical operation and the like can be judged more accurately, and the operation is simple and the use is convenient.
On the basis of the above embodiments, a detection channel is arranged in a detection tube in the zoom type cavity endoscope detection device provided by the embodiment of the invention, a light path channel is arranged in a middle through tube, the middle through tube is nested in the detection channel, a channel port of the detection channel is flush with a channel port of the light path channel, and a cover glass is arranged at the channel port of the detection channel, wherein:
The collimating lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror, the relay lens and the objective lens in the optical path structure are all positioned between the optical fiber universal interface of the handle shell and the passage port of the optical path passage;
The objective lens and the relay lens are arranged in the light path channel, and the objective lens is positioned at the channel opening of the light path channel. The middle through pipe in the zoom type cavity endoscope detection device provided by the embodiment of the invention is not only nested in the first transmission ring, but also nested in the detection channel of the detection device and the second transmission ring, the middle through pipe is internally provided with the optical path channel for forming the optical path structure of the first optical path and the second optical path to be positioned between the optical fiber universal interface in the handle shell and the channel port of the optical path channel, and the optical fiber universal interface is externally connected with various transmission optical fibers, wherein the objective lens and the relay lens which are shared by the first optical path and the second optical path are both arranged in the optical path channel, and the channel port of the detection channel is flush with the channel port of the optical path channel, so that the objective lens positioned at the channel port of the optical path channel is also positioned at the channel port of the detection channel at the same time, when the detection tube moves up and down relative to the handle shell, the objective lens arranged at the channel port of the optical path channel can move up and down relative to the cover glass arranged at the detection channel port, so that the distance between the objective lens and the cover glass can be adjusted according to the experimental needs to carry out tissue cell imaging of different depths to obtain the structure information of the tissue cells, thereby more accurately judging the infiltration depth of tumors, the transfer condition, the existence of cancer and the relative operation condition, the surgical operation condition is simple, and the surgical operation condition is convenient.
On the basis of the above embodiments, the optical path structure in the zoom type endoscope detecting device provided by the embodiment of the present invention further includes a liquid lens, and fig. 3 is a schematic diagram of a third structure of the zoom type endoscope detecting device provided by the embodiment of the present invention, as shown in fig. 3, the liquid lens 110 is located between the collimating lens 113 and the mems scanning galvanometer 114, so as to form a new first optical path, and the new first optical path sequentially includes the collimating lens 113, the liquid lens 110, the mems scanning galvanometer 114, the lens 115, the dichroic mirror and the objective lens 118. That is, the liquid lens 110 is arranged such that the liquid lens 110 is bent by applying a voltage or a current to the surface of the liquid lens 110, so that the parallel light emitted from the collimator lens 113 has different optical powers. The specific light path is: the laser signal is emitted from the optical fiber, is parallel to enter the liquid lens 110 after passing through the collimating lens 113, generates corresponding focal power according to the loaded voltage or current signal from the liquid lens 110, and the emitted converging or diverging light is transmitted to the objective lens 118 through the relay lens 117 by the micro-electromechanical scanning galvanometer 114, the lens and the dichroic mirror and then is converged on the sample. The focal power change introduced by the liquid lens 110 can enable the focal point of the laser signal emitted from the opening of the objective lens 118 to move back and forth in the depth direction, the response speed of the liquid lens 110 is very high, and the scanning frequency is in the order of KHz, so that rapid scanning imaging in the depth direction can be realized. The liquid lens 110 is equivalent to a parallel plate glass when no voltage or current signal is applied, and does not have optical power to the laser signal and does not cause any shift of the focus after the objective lens 118, thereby realizing three-dimensional stereoscopic imaging. When the zoom type endoscope detection device is specifically used, the liquid lens 110 is complementary to the zoom motor 119, the position of the objective lens 118 is adjusted through the zoom motor 119, after coarse adjustment to the corresponding depth position, the system is switched to a zoom scanning mode of the liquid lens 110, and rapid three-dimensional imaging is performed on a sample, wherein when the zoom type endoscope detection device is not provided with the zoom motor 119, zoom adjustment can be performed only through the liquid lens 110.
On the basis of the above embodiments, a plurality of illumination channels are further disposed in the detection tube in the zoom-type endoscope detection device according to the embodiment of the present invention, and fig. 4 is a schematic structural diagram of the zoom-type endoscope detection device according to the embodiment of the present invention, as shown in fig. 4, an illumination fiber bundle for transmitting illumination light signals is disposed in the illumination channel 123, where the illumination channels 123 are uniformly distributed with the axis of the detection channel as the center. That is, a plurality of illumination channels 123 are further arranged in a detection tube in the zoom type cavity endoscope detection device provided by the embodiment of the invention, more than one illumination channel 123 is arranged in each channel, each illumination channel is internally provided with an illumination fiber bundle, each illumination fiber bundle has a certain aperture angle, lenses are not required to be directly used for divergent illumination, the illumination channels 123 are uniformly distributed by taking the axle center of the detection channel as the center, and uniform illumination is provided for the zoom type cavity endoscope detection device so as to facilitate working observation of the state of a tissue region to be detected in front of an objective lens.
On the basis of the above embodiments, an observation channel is further disposed in a detection tube in the zoom-type endoscope detection device according to the embodiment of the present invention, as shown in fig. 4, the observation channel is located between the detection channel and the illumination channel, where:
An observation lens 122 is arranged at the channel opening of the observation channel, and the observation lens 122 is connected with a bright field optical fiber bundle in the observation channel and is used for acquiring image information of a tissue region to be detected in front of the objective lens. Namely, an observation channel is further arranged in a detection tube of the zoom type cavity endoscope detection device provided by the embodiment of the invention, the observation channel is positioned between the detection channel and the illumination channel, an observation lens 122 and a bright field optical fiber bundle are arranged, the bright field optical fiber bundle is an imaging optical fiber bundle and is used for transmitting image information of a tissue region to be detected before an objective lens captured by the observation lens 122, wherein one observation channel can be used or two observation channels can be used for forming binocular observation, and the function of the three-dimensional bright field cavity endoscope is realized.
On the basis of the above embodiments, an adsorption channel is further disposed in the probe tube in the zoom-type endoscope probe device according to the embodiments of the present invention, as shown in fig. 4, where the adsorption channel 124 is located between the illumination channel and the edge of the probe tube. That is, the detection tube in the zoom type cavity endoscope detection device provided by the embodiment of the invention is also provided with the adsorption channel 124 for adsorbing the zoom type cavity endoscope detection device on the tissue to be detected, and negative pressure is formed in the adsorption channel 124 by extracting air in the adsorption channel 124, so that the zoom type cavity endoscope detection device is adsorbed on the tissue to be detected, wherein the adsorption channel 124 is positioned between the illumination channel and the edge of the detection tube, namely, at a position which is positioned outside the illumination channel and is close to the side of the detection tube.
On the basis of the above embodiments, a button hole is formed in a handle housing of the zoom type endoscope detection device provided by the embodiment of the invention, and a switching button and an imaging button are arranged in the button hole, wherein the switching button is used for switching different optical filters so as to obtain illumination light signals with different wavelengths;
The imaging button is used for controlling an imaging module connected with the bright field optical fiber bundle to image the tissue region to be detected in front of the objective lens. Namely, a switching button is arranged in a button hole of a handle shell in the zoom type cavity endoscope detection device provided by the embodiment of the invention, and an optical filter for filtering illumination light signals with different wavelengths can be switched through the switching button, so that a worker can select the transmitted illumination light signals with different wavelengths; an imaging button is arranged in a button hole of a handle shell in the zoom type cavity endoscope detection device, an imaging module connected with a bright field optical fiber bundle can be controlled to shoot and image a tissue region to be detected in front of an objective lens through the imaging button, and functions of the switching button and the imaging button can be customized through software to modify corresponding functions.
The embodiment of the invention also provides a three-dimensional nonlinear laser scanning cavity endoscope, fig. 5 is a schematic structural diagram of the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention, as shown in fig. 5, the three-dimensional nonlinear laser scanning cavity endoscope includes:
fluorescence collection device 56, scan collection controller 531, femtosecond pulse laser, fiber coupling module, and the varifocal cavity endoscope detection device 1 that each of the above-mentioned embodiments provided, fluorescence collection device 56 and fiber coupling module all are connected with varifocal cavity endoscope detection device 1 fiber communication, and fluorescence collection device and varifocal cavity endoscope detection device all are connected with scan collection controller 531 electricity, wherein:
The femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;
The optical fiber coupling module is used for coupling the pulse laser signals output by the femtosecond pulse laser and transmitting the pulse laser signals to a collimating lens in the zoom type cavity endoscope detection device 1;
the zoom type cavity endoscope detecting device 1 is used for receiving the pulse laser signal, outputting the pulse laser signal to an autofluorescent substance in a living body cell, acquiring a fluorescence signal and a second harmonic signal generated after the autofluorescent substance is excited through an objective lens, and outputting the fluorescence signal and the second harmonic signal to the fluorescence collecting device 56;
The fluorescence collection device 56 is configured to convert the fluorescence signal and the second harmonic signal into corresponding electrical signals after receiving the fluorescence signal and the second harmonic signal, respectively;
and the scanning acquisition controller 531 is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signal and synchronously acquire the electric signal.
Specifically, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention comprises a fluorescence collection device 56, a scanning acquisition controller 531, a femtosecond pulse laser, an optical fiber coupling module and a zoom type cavity endoscope detection device 1, so that the three-dimensional nonlinear laser scanning cavity endoscope which detects human gastrointestinal tissues and oral tissues by utilizing a two-photon imaging technology is formed, wherein the femtosecond pulse laser can emit pulse laser signals for exciting autofluorescent substances in human gastrointestinal tissues and oral tissue cells to generate multiphoton fluorescence signals and second harmonic signals, the femtosecond pulse laser with the wavelength of 920nm is used for exciting FAD and collagen in cells, the fluorescence signals with the wavelength of 500-600nm and the second harmonic signals with the wavelength of 460nm are excited, and the autofluorescent substances such as FAD or NADH in cells are excited by the femtosecond pulse laser with the wavelength of 780nm to generate corresponding fluorescence signals and second harmonic signals, wherein the femtosecond pulse laser and the optical fiber coupling module are combined together to form a laser emission module 540;
the fluorescence collection device 56 integrates two signal collection light paths, namely a fluorescence signal collection light path and a second harmonic signal collection light path, so as to realize the collection of fluorescence signals and second harmonic signals respectively; the scanning acquisition controller 531 controls the micro-electromechanical scanning galvanometer to scan the pulse laser signal and excite the autofluorescent substance to generate a fluorescent signal and a second harmonic signal, and acquires a first electric signal and a second electric signal obtained by converting the fluorescent signal and the second harmonic signal by the fluorescent collection device; the three-dimensional nonlinear laser scanning endoscope is divided into a laparoscope and a stomatoscope according to the difference of the structures of the zoom type endoscope detecting device 1. The resolution of the three-dimensional nonlinear laser scanning cavity endoscope can be set to 800nm, the imaging field of view can be 400 micrometers by 400 micrometers, and the imaging speed can be 26 frames (256×256 pixels) or 13 frames (512×512 pixels).
The three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention adopts the fluorescence collection device, the scanning acquisition controller, the femtosecond pulse laser, the optical fiber coupling module and the zooming cavity endoscope detection device, so that the laser scanning cavity endoscope which utilizes the two-photon imaging technology to detect human gastrointestinal tissues and oral cavity tissues is formed, the focal length of the objective lens is adjusted through the liquid lens and the zooming motor, the three-dimensional scanning of the laser scanning microscope on cell structures with different depths is realized, the fluorescence signals and the second harmonic signals of the cells are obtained by exciting the autofluorescent substances in the cells through the femtosecond pulse laser, the nonlinearity of the laser scanning microscope is realized, the fluorescence signals and the second harmonic signals are collected through the fluorescence collection device and are converted into corresponding electric signals, and then the corresponding fluorescence images reflecting the cell tissue structures and the like are obtained through the electric signals.
Based on the above embodiments, the three-dimensional nonlinear laser scanning endoscope provided by the embodiments of the present invention further includes an illumination module and an imaging module, as shown in fig. 5, where the illumination module 534 and the imaging module 533 are connected in optical fiber communication with the zoom type endoscope detection device, where:
The illumination module 534 sequentially includes an illumination lens 5342, a variable filter 5341, and an illumination light source 5343, the illumination lens 5342 being connected to the illumination fiber bundle, the illumination light source for providing an illumination light signal;
The imaging module 533 sequentially includes an imaging lens 5331 and a camera 5332, wherein the imaging lens 5331 is connected to the bright field optical fiber bundle, and the camera 5332 is used for acquiring image information of a tissue region to be detected. Namely, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention is further provided with an illumination module 534 and an imaging module 533, wherein the illumination module 534 sequentially comprises an illumination lens 5342, a variable optical filter 5341 and an illumination light source 5343, wherein the illumination light source can switch different optical filters through an electric variable optical filter rotating wheel to obtain illumination light signals with different wavelengths, and the basic principle is that when two-photon fluorescence imaging is not interfered, for example, autofluorescence and second harmonic waves are obtained, the three-dimensional nonlinear laser scanning cavity endoscope can be switched to a red or infrared optical filter to obtain illumination light signals with 370nm, 635nm or infrared 850nm and 940nm, and the illumination light signals are coupled into an illumination optical fiber bundle through the lens;
The imaging module 533 in turn includes an imaging lens and a camera, the lens focusing on the camera for imaging for direct viewing of the bright field. The two cameras correspond to the binocular field-improving optical fiber bundles, the multi-mode laparoscope is formed by field-improving imaging and two-photon imaging, the large-field sample observation is carried out by the field-improving binocular three-dimensional laparoscope mode, and the basic morphology of the sample is mainly observed. For a suspicious or interested area, the method can be switched to a two-photon mode to perform autofluorescence and second harmonic imaging, and observe the cell-level morphology of a sample, so that basis is provided for further judgment, wherein a camera can be imaging equipment based on CCD or CMOS imaging devices.
On the basis of the above embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention further comprises an air extracting device, as shown in fig. 5, the air extracting device 52 mainly comprises an air extracting pump, the air extracting device is connected with the adsorption channel through an air extracting pipeline, an air extracting valve is arranged in the air extracting pipeline and is electrically connected with the air extracting device 52, the air extracting device 52 controls the air extracting flow of the air extracting pipeline by adjusting the opening and closing of the air extracting valve, so that the air extracting control of the adsorption channel is realized, the negative pressure in the adsorption channel is further adjusted, the zoom type cavity endoscope detecting device is adsorbed on tissues such as stomach intestine, mouth cavity and uterine cavity in the abdominal cavity of a human body under the action of atmospheric pressure, and motion artifacts caused by the movement of biological tissues are reduced, so that imaging is more stable and clear.
Based on the above embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiments of the present invention further includes an industrial personal computer, as shown in fig. 5, wherein the industrial personal computer 532 is electrically connected with the scanning acquisition controller 531, where:
The industrial personal computer 532 is configured to acquire the first electrical signal and the second electrical signal acquired by the scan acquisition controller 531, generate a first fluorescent image based on the first electrical signal, and generate a second fluorescent image based on the second electrical signal. That is, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention further comprises an industrial personal computer 532 electrically connected with the scanning acquisition controller 531, the industrial personal computer 532 generates a first fluorescent image based on a first electric signal and generates a second fluorescent image based on a second electric signal, and the first fluorescent image and the second fluorescent image can be respectively used for displaying cell structure and fiber structure information, wherein control software is installed on the industrial personal computer, and a control instruction is sent to the scanner through the control software so as to control the scanning acquisition controller to acquire the first electric signal and the second electric signal.
Based on the above embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiments of the present invention further includes a display, as shown in fig. 5, where the display 55 is electrically connected to the industrial personal computer 532, and is configured to display a first fluorescent image and a second fluorescent image. That is, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention further comprises a display 55 for displaying the first fluorescent image and the second fluorescent image, and a worker can directly acquire related information of the first fluorescent image and the second fluorescent image through the display 55.
Fig. 6 is a schematic diagram of a three-dimensional nonlinear laser scanning cavity endoscope according to a second embodiment of the present invention, where, as shown in fig. 6, the three-dimensional nonlinear laser scanning cavity endoscope also includes:
The fluorescence collection device 56, the scan collection controller 531, the femtosecond pulse laser, the optical fiber coupling module, and the zoom type cavity endoscope detection device 1, the air extraction device 52, the industrial personal computer 532, the illumination module 534, and the imaging module 533 provided in the above embodiments, wherein the fluorescence collection device 56 and the optical fiber coupling module are all connected with the zoom type cavity endoscope detection device 1 through optical fiber communication, the fluorescence collection device 56 and the zoom type cavity endoscope detection device 1 are all electrically connected with the scan collection controller 531, the functions of the above modules or devices are the same as those of the above embodiments, the combination of the femtosecond pulse laser and the optical fiber coupling module forms the laser emission module 540, the illumination module 534 sequentially comprises the illumination lens 5342, the variable optical filter 5341, and the illumination light source 5343, the imaging module 533 sequentially comprises the imaging lens and the camera, the zoom type cavity endoscope detection device 1 in the three-dimensional nonlinear laser scan cavity endoscope is the cavity endoscope detection device, the liquid lens is contained in the optical path structure of the zoom type cavity endoscope detection device, and the functions the liquid lens is the same as the liquid lens contained in the above embodiments, and the optical path is the same as that of the liquid lens contained in the above embodiments and the optical path corresponds to the above embodiments.
On the basis of the above embodiments, fig. 7 is a schematic structural diagram of a fluorescence collection device according to an embodiment of the present invention, as shown in fig. 7, where the fluorescence collection device according to an embodiment of the present invention includes a collection fiber universal interface 881, a first photomultiplier 882, a second photomultiplier 883, a first collection optical path located between the collection fiber universal interface 881 and the first photomultiplier 882, and a second collection optical path located between the collection fiber universal interface 881 and the second photomultiplier 883, wherein:
The first collecting light path sequentially comprises a coupling lens 81, an infrared filter 82, a first dichroic mirror 83, a first filter 84 and a first collecting lens 85, wherein the first collecting light path is used for collecting fluorescent signals received by the fluorescent collecting device, and the first photomultiplier 882 is used for converting the fluorescent signals into first electric signals;
The second collecting light path sequentially includes a coupling lens 81, an infrared filter 82, a first dichroic mirror 83, a second dichroic mirror 86, a second filter 87, and a second collecting lens 88, where the second collecting light path is used to collect the second harmonic signal received by the fluorescent collecting device, and the second photomultiplier 883 is used to convert the second harmonic signal into a second electric signal. Namely, the fluorescence collection device provided by the embodiment of the invention has a double-path signal collection function, and integrates two paths of light paths, wherein the first dichroic mirror 83 in the first collection light path is a dichroic mirror for transmitting fluorescence signals, reflecting second harmonic waves, the second dichroic mirror 86 and the first dichroic mirror 83 are the same dichroic mirror for reflecting the second harmonic waves, the first filter 84 is used for transmitting fluorescence signals and filtering out other interference signals, the second filter 87 is used for transmitting corresponding second harmonic signals and filtering out other interference signals, for example, when 780nm femtosecond fiber lasers are used for exciting autofluorescence substances in abdominal cavities or oral cells of a human body, 390nm second harmonic signals and 450-600nm two-photon autofluorescence signals can be obtained, two paths of fluorescence can be separated through the dichroic mirror which is reflecting the second harmonic waves with more than 420nm, namely the first dichroic mirror 83, and the first filter 84 with 390+/-20 nm and the second filter 87 with 450-600nm can be used for obtaining clean second harmonic signals and fluorescence signals respectively.
Fig. 8 is a schematic diagram of a box-type combined structure of a three-dimensional nonlinear laser scanning endoscope in a box-type combined structure, as shown in fig. 8, a display 55 integrated on a box cover is integrated with a box body provided with each module, so that the whole equipment can be conveniently moved and a workplace can be conveniently replaced, and the display 55 can be externally placed on the box body when in use, so that a worker can conveniently obtain information on the display, wherein a zoom type endoscope detection device 1 in the three-dimensional nonlinear laser scanning endoscope is an stomatoscope detection device. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box, and the equipment can be conveniently replaced in a workplace, especially in a hospital, a laboratory or an outdoor place.
Fig. 9 is a schematic diagram of a box-type combined structure of a three-dimensional nonlinear laser scanning endoscope, as shown in fig. 9, a display 55 integrated on a box cover is integrated with a box body provided with each module, so that the whole equipment can be conveniently moved and a workplace can be replaced, and the display 55 can be externally placed on the box body when in use, so that a worker can conveniently obtain information on the display, wherein a zoom type endoscope detection device 1 in the three-dimensional nonlinear laser scanning endoscope is a laparoscope detection device, and a plurality of laparoscope detection devices can be simultaneously arranged. After the three-dimensional nonlinear laser scanning cavity endoscope is used, a worker can carry the equipment box, and the equipment can be conveniently replaced in a workplace, especially in a hospital, a laboratory or an outdoor place.
Based on the above embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiment of the invention has a plurality of zoom type cavity endoscope detection devices. The fluorescence collection device and the optical fiber coupling module provided by the embodiment of the invention can be simultaneously connected with a plurality of zoom type cavity endoscope detection devices in an optical fiber communication way, namely, a plurality of detection devices are integrated in a three-dimensional nonlinear laser scanning cavity endoscope system, so that the simultaneous detection of different parts of gastrointestinal tissues is realized, and the contrast analysis is performed.
Based on the above embodiments, the three-dimensional nonlinear laser scanning cavity endoscope provided by the embodiments of the present invention further includes an adjusting optical fiber, which is used for optical fiber transmission connection between the fluorescence collection device and the optical fiber coupling module and the zoom cavity endoscope detection device, respectively, wherein:
The length of the adjusting optical fiber is adjustable. The optical fiber coupling module is connected with the optical fiber transmission device through the optical fiber with adjustable length, so that the detection device can be flexibly moved according to different experimental scene requirements, the limit of the limited optical fiber length is avoided, the length of the adjusting optical fiber can be adjusted, the application of various occasions can be realized by changing the optical fibers with different lengths, and the optical fiber with different lengths can be changed at any time according to the requirements.
For the three-dimensional nonlinear laser scanning cavity endoscope provided by the above embodiments, another specific implementation manner is provided in the embodiment of the present invention, fig. 10 is a schematic diagram of a desk-top structure of the three-dimensional nonlinear laser scanning cavity endoscope provided in the embodiment of the present invention, as shown in fig. 10, the three-dimensional nonlinear laser scanning cavity endoscope includes an air extraction device 52, a first device 53, a second device 54, a display 55, and a zoom cavity endoscope detection device 1, where a scan acquisition controller and an industrial personal computer are integrated in the first device 53, the industrial personal computer is electrically connected with the display 55, the second device 54 is integrated with a femtosecond pulse laser, an optical fiber coupling module, a fluorescence collection device, an illumination module, and an imaging module, and the optical fiber coupling module and the fluorescence collection device are all connected with an adsorption type microscope detection device 51 in an optical fiber transmission manner, where the zoom cavity endoscope detection device 1 is an oral cavity mirror detection device for detecting the information of malignancy, infiltration depth, transfer condition, and presence or absence of cancer residues of a cutting edge of a tumor, and the operation of the adsorption type three-dimensional nonlinear laser microscope is the same as the above embodiments.
The embodiment of fig. 11 provides a schematic diagram of a desk-top structure of a three-dimensional nonlinear laser scanning cavity endoscope, as shown in fig. 11, the three-dimensional nonlinear laser scanning cavity endoscope also includes an air extractor 52, a first device 53, a second device 54, a display 55 and a zoom type cavity endoscope detection device 1, wherein the first device 53 is integrated with a scanning acquisition controller and an industrial personal computer, the industrial personal computer is electrically connected with the display 55, the second device 54 is integrated with a femtosecond pulse laser, an optical fiber coupling module, a fluorescence collection device, an illumination module and an imaging module, the optical fiber coupling module and the fluorescence collection device are all in optical fiber transmission connection with the adsorption type microscope detection device 51, the zoom type cavity endoscope detection device 1 is a laparoscope detection device, the laparoscope detection device is embedded into the abdomen of a human body, and is used for detecting gastrointestinal tissues so as to know information such as malignancy of tumors, infiltration depth, transfer conditions, presence or absence of cancer residues at a cutting edge, and the laparoscope based on the laparoscope of the laparoscope detection device can also be used for detecting female intrauterine tissues, and the detection tissue imaging principle is the same as that of the cavity in the above embodiments.
While the present application has been described in connection with the embodiments of the present application, it will be understood by those skilled in the art that the present application is not limited to the preferred embodiments of the present application, and various modifications and variations can be made thereto by those skilled in the art, based on the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
The apparatus embodiments described above are merely illustrative, wherein elements illustrated as separate elements may or may not be physically separate, and elements shown as elements may or may not be physical elements, may be located in one place, or may be distributed over a plurality of network elements. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment. Those of ordinary skill in the art will understand and implement the present invention without undue burden.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A zoom type endoscope probe device, comprising:
Handle casing and probe tube, handle casing bottom is provided with well siphunculus and first transmission ring, the probe tube top is provided with the second transmission ring, first transmission ring with the second transmission ring rotates to be connected, well siphunculus nestification is in the probe tube with in the first transmission ring, be provided with the motor that zooms in the handle casing and be used for forming the light path structure of first light path and second light path, wherein:
The zoom motor is used for driving the second transmission ring to move up and down relative to the first transmission ring, and comprises up and down movement in a relative rotation mode and vertical up and down movement in a direct drive mode;
the first optical path sequentially comprises a collimating lens, a micro-electromechanical scanning galvanometer, a lens, a dichroic mirror, a relay mirror and an objective lens, wherein the first optical path is used for conducting laser signals received by the collimating lens from the collimating lens to the objective lens;
The second optical path sequentially comprises the objective lens, the relay lens and the dichroic mirror, wherein the second optical path is used for conducting optical signals collected by the objective lens from the objective lens to the dichroic mirror.
2. The zoom type endoscope detection device according to claim 1, wherein a detection channel is provided in the detection tube, an optical path channel is provided in the middle through tube, the middle through tube is nested in the detection channel, a channel opening of the detection channel is flush with a channel opening of the optical path channel, and a cover glass is provided at the channel opening of the detection channel, wherein:
the collimating lens, the micro-electromechanical scanning galvanometer, the lens, the dichroic mirror, the relay lens and the objective lens in the optical path structure are all positioned between the optical fiber universal interface of the handle shell and the channel port of the optical path channel;
the objective lens and the relay lens are both arranged in the light path channel, and the objective lens is positioned at the channel opening of the light path channel.
3. The variable focal length cavity endoscopic probe apparatus of claim 1 or 2, wherein the optical path structure further comprises a liquid lens positioned between the collimating lens and the microelectromechanical scanning galvanometer to form a new first optical path comprising, in order, the collimating lens, the liquid lens, the microelectromechanical scanning galvanometer, the lens, the dichroic mirror, and the objective lens.
4. The zoom type endoscope detection device according to claim 2, wherein a plurality of illumination channels are further arranged in the detection tube, illumination fiber bundles for transmitting illumination light signals are arranged in the illumination channels, and the illumination channels are uniformly distributed with the axis of the detection channels as the center.
5. The variable focus type cavity endoscope detection apparatus according to claim 4, wherein an observation channel is further provided in the detection tube, the observation channel being located between the detection channel and the illumination channel, wherein:
An observation lens is arranged at the channel opening of the observation channel and connected with the bright field optical fiber bundle in the observation channel, so as to acquire image information of the tissue region to be detected in front of the objective lens.
6. The variable focal length endoscope probe device of claim 4, wherein an adsorption channel is further disposed within the probe tube, the adsorption channel being located between the illumination channel and the probe tube edge.
7. The zoom type endoscope detection device according to claim 5, wherein a button hole is formed in the handle housing, a switching button and an imaging button are arranged in the button hole, and the switching button is used for switching different optical filters so as to obtain the illumination light signals with different wavelengths;
the imaging button is used for controlling an imaging module connected with the bright field optical fiber bundle to image the tissue region to be detected in front of the objective lens.
8. A three-dimensional nonlinear laser scanning cavity endoscope, comprising:
A fluorescence collection device, a scanning acquisition controller, a femtosecond pulse laser, an optical fiber coupling module, and the zoom type cavity endoscope detection device of any one of claims 1-7, wherein the fluorescence collection device and the optical fiber coupling module are both in optical fiber communication connection with the zoom type cavity endoscope detection device, and the fluorescence collection device and the zoom type cavity endoscope detection device are both electrically connected with the scanning acquisition controller, wherein:
The femtosecond pulse laser is used for outputting pulse laser signals to the optical fiber coupling module;
The optical fiber coupling module is used for coupling the pulse laser signals output by the femtosecond pulse laser and transmitting the pulse laser signals to the collimating lens in the zoom type cavity endoscope detection device;
The zoom type cavity endoscope detection device is used for receiving the pulse laser signals, outputting the pulse laser signals to autofluorescent substances in living cells, acquiring fluorescent signals and second harmonic signals generated after the autofluorescent substances are excited through the objective lens, and outputting the fluorescent signals and the second harmonic signals to the fluorescent collection device;
The fluorescence collection device is used for respectively converting the fluorescence signal and the second harmonic signal into corresponding electric signals after receiving the fluorescence signal and the second harmonic signal;
the scanning acquisition controller is used for controlling the micro-electromechanical scanning galvanometer to scan the pulse laser signals and synchronously acquiring the electric signals.
9. The three-dimensional nonlinear laser scanning cavity endoscope of claim 8, further comprising an illumination module and an imaging module, both in optical fiber communication with the cavity endoscopic probe apparatus, wherein:
The illumination module sequentially comprises an illumination lens, a variable optical filter and an illumination light source, wherein the illumination lens is connected with the illumination optical fiber bundle, and the illumination light source is used for providing illumination light signals;
the imaging module sequentially comprises an imaging lens and a camera, wherein the imaging lens is connected with the bright field optical fiber bundle, and the camera is used for acquiring image information of a tissue region to be detected.
10. The three-dimensional nonlinear laser scanning cavity endoscope of claim 8, wherein the fluorescence collection device comprises a collection fiber optic universal interface, a first photomultiplier tube, a second photomultiplier tube, and a first collection optical path between the collection fiber optic universal interface and the first photomultiplier tube, a second collection optical path between the collection fiber optic universal interface and the second photomultiplier tube, wherein:
The first collecting light path sequentially comprises a coupling lens, an infrared filter, a first dichroic mirror, a first filter and a first collecting lens, wherein the first collecting light path is used for collecting the fluorescent signals received by the fluorescent collecting device, and the first photomultiplier is used for converting the fluorescent signals into first electric signals;
the second collecting light path sequentially comprises the coupling lens, the infrared filter, the first dichroic mirror, the second filter and the second collecting lens, wherein the second collecting light path is used for collecting the second harmonic signals received by the fluorescent collecting device, and the second photomultiplier is used for converting the second harmonic signals into second electric signals.
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| CN113040904B (en) * | 2021-03-16 | 2024-10-01 | 深圳市福瑞光电子技术有限责任公司 | Adsorption laser operation endoscope |
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