CN109744234A - A kind of insecticide for controlling diamondback moth and its screening method - Google Patents
A kind of insecticide for controlling diamondback moth and its screening method Download PDFInfo
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- CN109744234A CN109744234A CN201811497634.7A CN201811497634A CN109744234A CN 109744234 A CN109744234 A CN 109744234A CN 201811497634 A CN201811497634 A CN 201811497634A CN 109744234 A CN109744234 A CN 109744234A
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- insecticide
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Abstract
本发明涉及一种防治小菜蛾的杀虫剂及其筛选方法，所述杀虫剂由以下原料组成：丁香油酚(Eugenol)、香茅烷(Myrcane)和柠烯(Limonene)这三种植物源提取物，所述丁香油酚(Eugenol)、香茅烷(Myrcane)和柠烯(Limonene)的体积比为1‑40:20‑50:20‑50混合均匀得到杀虫成分。本发明提供了一种植物源剂提取物对小菜蛾进行杀虫、趋避的新用途，且杀虫效果显著。The invention relates to an insecticide for controlling diamondback moth and a screening method thereof. The insecticide is composed of the following raw materials: Eugenol, Myrcane and Limonene. The source extract, the volume ratio of Eugenol (Eugenol), citronellane (Myrcane) and limonene (Limonene) is 1-40:20-50:20-50 and evenly mixed to obtain insecticidal components. The invention provides a new application of the plant source agent extract for killing and avoiding the diamondback moth, and the insecticidal effect is remarkable.
Description
Technical field
The present invention relates to a kind of insecticide and its screening techniques, and in particular to a kind of insecticide and its sieve for preventing and treating diamondback moth
Choosing method.
Background technique
Diamondback moth (Plutella xylostella Linnaeus) belongs to lepidoptera diamond-back moth section.The worldwide pest that migrates, it is main
Cause harm the crucifers such as wild cabbage, violet cabbage.Based on larval feeding blade, first instar larvae only feeding mesophyll, 3 is more than age
Dish leaf is eaten into hole and is incised by larva, and blade reticulates when serious.Seedling stage often concentrates lobus cardiacus of causing harm, and strain of reserving seed for planting mainly is caused harm tender
Stem, young pod and seed etc..
Diamondback moth prevention and treatment at present is mainly lured with lamp not significant with biological agent effect, and chemical prevention is quick, but long-term, big
The use of amount, the problems such as having caused many ecological environment and human health.The medicament of plant source has and easily decomposes, nonhazardous,
Decomposition product will not generate harm to Man & Nature, and Extracts from Plant Recourses is to the mode of action of pest multiplicity, including repellent, refuses
Eat, lure, tagging, inhibiting growth and development etc., and draw materials extensively, what can be extracted from the flower of plant, fruit, leaf, stem, root waves
The grease of hair property and fragrance, is widely present in nature, containing oxygen derivative in the form of terpenes and containing oxygen derivative
Mainly the forms such as alcohol, aldehyde, ketone, carboxylic acid, ester and glycosides exist.In recent years, related plants essential oil is killed as environmentally protective both at home and abroad
Worm agent is applied to the study on prevention of each class pest, makes great progress.
Summary of the invention
To solve prior art deficiency, the present invention provides a kind of insecticides and its screening technique for preventing and treating diamondback moth, originally
Invention has been designed to provide a kind of new application that Extracts from Plant Recourses carries out desinsection to diamondback moth, walks quickly and keeps away.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of insecticide for preventing and treating diamondback moth, the insecticide includes three kinds of Eugenol, lemongrass alkane and hesperidene plant sources
Extract.
Further, the volume ratio of the Eugenol, lemongrass alkane and hesperidene is 1-40:20-50:20-50.
The present invention also provides a kind of screening techniques of insecticide for preventing and treating diamondback moth, include the following steps:
S1, using different types of Extracts from Plant Recourses, by each Extracts from Plant Recourses individually or according to not androgynous
Product proportion, which is combined, prepares multiple groups botanical composition；
S2, botanical composition prepared in step S1 is dissolved in organic solvent respectively, obtains the plant of respective components
Material resource insecticide；
S3, the plant insecticide for taking different volumes respectively are added in a certain number of diamondback moths, and record is different
The death rate of time diamondback moth；
S4, according to the death rate of diamondback moth in step S3, to choose best Extracts from Plant Recourses combination, Extracts from Plant Recourses
Proportion and certain diamondback moth quantity needed for best plant insecticide volume fraction.
Further, the Extracts from Plant Recourses is selected from Eugenol, lemongrass alkane, hesperidene, geranyl acetate and australene
One or more.
Further, in step S2, any one or two kind of the organic solvent in methanol and acetone.
Further, in step S3, the volume fraction of taken plant insecticide is 5-10 μ l/ML.
The beneficial effects of the present invention are, the present invention provides a kind of plant source agent extract to diamondback moth carry out desinsection,
The new application walked quickly and kept away, and insecticidal effect is significant.
Specific embodiment
Following illustrated embodiment is served only for explaining the present invention, is not intended to limit the scope of the present invention.
It should be noted that the Extracts from Plant Recourses is purchased from uncommon love (Shanghai) chemical conversion industry of ladder in following embodiment
Development Co., Ltd, purity refrigerate spare 95% or more.
Diamondback moth (Plutella xylostella (Linnaeus)) Hubei Engineering University characteristic fruits and vegetables quality and safety control
Raising obtains in key lab, Hubei Province.Terpenoid, which has, easily to be decomposed, and nonhazardous, decomposition product is to Man & Nature without danger
It includes plant itself and compound and derivative by active structure synthesis that evil, which is using the active constituent in plant, exempts from it
By or mitigate the plant source preparation that the harmful organisms such as disease pest cause harm.
Extracts from Plant Recourses Eugenol (Eugenol), lemongrass alkane (Myrcane), hesperidene (Limonene), acetic acid is fragrant
Leaf ester (Geranyl acetate) and australene (α-Pinene) are indicated with A, B, C, D and E respectively.
Embodiment 1
Take the Extracts from Plant Recourses Eugenol of same volume, Myrcane, Limonene, Geranyl acetate and
Australene takes 5 μ l Extracts from Plant Recourses to be dissolved in the organic solvent of 1ML respectively respectively and obtains plant insecticide, is added to and puts
In the port grinding bottle for having the 1000ml of the diamondback moth of identical quantity, 12,24,36 hours diamondback moth The dead quantities are recorded.As a result such as table 1
It is shown.
Median lethal rate of the 1 five kinds of plant insecticides of table to diamondback moth
As it can be seen from table 1 these four plant sources of Eugenol, Myrcane, Limonene and Geranyl acetate kill
Worm agent is in 60% or more to diamondback moth lethality.
Embodiment 2
Lethality of two kinds of insecticide mixing to diamondback moth
Extracts from Plant Recourses Eugenol, Myrcane, Limonene and Geranyl acetate of different volumes is taken,
According to the proportion in table 2, take respectively 5 μ l Extracts from Plant Recourses and be dissolved in respectively 1ML methanol and acetone it is one or two kinds of mixed
It closes in solution and obtains plant insecticide, be added in the port grinding bottle for the 1000ml of diamondback moth for being placed with identical quantity, record 12,
24,36 hours diamondback moth The dead quantities, the results are shown in Table 2.
2 four kinds of insecticides of table match the median lethal rate for comparing diamondback moth larvae two-by-two
From table 2 it can be seen that insecticidal effect is significantly higher than a kind of effect of insecticide when A:B is 1:1.
Embodiment 3
Extracts from Plant Recourses Eugenol, Myrcane, Limonene and Geranyl acetate of different volumes is taken,
According to the proportion in table 3,5 μ l Extracts from Plant Recourses is taken to be dissolved in methanol and the one or two kinds of mixing of acetone of 1ML respectively respectively
Plant insecticide is obtained in solution, is added in the port grinding bottle for the 1000ml of diamondback moth for being placed with identical quantity, record 12,
24,36 hours diamondback moth The dead quantities, the results are shown in Table 3.
Median lethal rate of the 3 four kinds of insecticides of table to three or three proportion diamondback moth larvaes
From table 3 it can be seen that insecticidal effect is higher than a kind of insecticide when A:B:C is that 1:1:1 or A:B:C=1:2:2 is mixed
Or the mixed effect of two kinds of insecticides.
Embodiment 4
Extracts from Plant Recourses Eugenol, Myrcane, Limonene and Geranyl acetate of different volumes is taken,
According to the proportion in table 4,5 μ l Extracts from Plant Recourses are taken respectively and are dissolved in the methanol and acetone mixed solution of 1ML respectively obtain
Plant insecticide is added in the port grinding bottle for the 1000ml of diamondback moth for being placed with identical quantity, is recorded 12,24,36 hours small
Diamond-back moth The dead quantity, the results are shown in Table 4.
Median lethal rate of the 4 four kinds of insecticide different ratios of table to diamondback moth larvae
From table 4, it can be seen that insecticidal effect is significantly higher than a kind of insecticide or equivalent when A:B:C:D is that 1:1:2:2 is mixed
In the effect of two kinds of insecticides.
Embodiment 5
It can be obtained from embodiment 1-4, best Extracts from Plant Recourses is Eugenol, Myrcane, Limonene, according to table 5
In proportion, take 10 μ l Extracts from Plant Recourses respectively and be dissolved in the methanol of 1ML respectively and acetone is one or two kinds of is mixed with
Plant insecticide is obtained in solvent, is added in the port grinding bottle for the 1000ml of diamondback moth for being placed with identical quantity, record 12,
24,36 hours diamondback moth The dead quantities, the results are shown in Table 5.
The median lethal rate of 5 three kinds of insecticide different ratio diamondback moth larvaes of table
As can be seen from Table 5, when A:B:C is that 1:20:20 or A:B:C=40:50:50 is mixed, insecticidal effect is significant.
According to the test of embodiment 1-5 as can be seen that Extracts from Plant Recourses is to the lethal effect optimum formula of diamondback moth,
According to the volume ratio of Extracts from Plant Recourses Eugenol, Myrcane and Limonene are as follows: 1-40:20-50:20-50 takes plant
Source extract is simultaneously dissolved in the methanol of 1ML and the in the mixed solvent that formaldehyde is one or two kinds of, under a certain amount of diamondback moth quantity, takes 5-
The insecticidal effect of 10 μ l is best.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of insecticide for preventing and treating diamondback moth, which is characterized in that the insecticide includes Eugenol, lemongrass alkane and hesperidene three
Kind Extracts from Plant Recourses.
2. preventing and treating the insecticide of diamondback moth according to claim 1, which is characterized in that the Eugenol, the lemongrass alkane
Volume ratio with the hesperidene is 1-40:20-50:20-50.
3. a kind of screening technique for the insecticide for preventing and treating diamondback moth, which comprises the steps of:
S1, using different types of Extracts from Plant Recourses, each Extracts from Plant Recourses individually or according to different volumes is matched
Multiple groups botanical composition is prepared than being combined；
S2, botanical composition prepared in step S1 is dissolved in organic solvent respectively, obtains the plant source of respective components
Insecticide；
S3, the plant insecticide for taking different volumes respectively are added in a certain number of diamondback moths, record different time
The death rate of diamondback moth；
S4, according in step S3, the death rate of diamondback moth, to choose best Extracts from Plant Recourses combination, Extracts from Plant Recourses
The volume fraction of best plant insecticide needed for proportion and certain diamondback moth quantity.
4. preventing and treating the screening technique of the insecticide of diamondback moth according to claim 3, which is characterized in that the plant source extracts
Object is selected from the one or more of Eugenol, lemongrass alkane, hesperidene, geranyl acetate and australene.
5. preventing and treating the screening technique of the insecticide of diamondback moth according to claim 3, which is characterized in that described in step S2
Any one or two kind of the organic solvent in methanol and acetone.
6. preventing and treating the screening technique of the insecticide of diamondback moth according to claim 3, which is characterized in that in step S3, taken
The volume fraction of plant insecticide is 5-10 μ l/ML.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811497634.7A CN109744234A (en) | 2018-12-07 | 2018-12-07 | A kind of insecticide for controlling diamondback moth and its screening method |
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| CN201811497634.7A CN109744234A (en) | 2018-12-07 | 2018-12-07 | A kind of insecticide for controlling diamondback moth and its screening method |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322110A (en) * | 1998-07-28 | 2001-11-14 | 艾科斯迈特技术公司 | Synergistic and residual pesticidal compositions contaiing plant essential oils |
| CN1124073C (en) * | 1998-01-27 | 2003-10-15 | 西麦德集团公开有限公司 | Terpene treatments for killing lice and lice eggs |
-
2018
- 2018-12-07 CN CN201811497634.7A patent/CN109744234A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124073C (en) * | 1998-01-27 | 2003-10-15 | 西麦德集团公开有限公司 | Terpene treatments for killing lice and lice eggs |
| CN1322110A (en) * | 1998-07-28 | 2001-11-14 | 艾科斯迈特技术公司 | Synergistic and residual pesticidal compositions contaiing plant essential oils |
Non-Patent Citations (3)
| Title |
|---|
| CLAUDINEI ANDRADE FILOMENO等: "Corymbiaspp. and Eucalyptus spp. essential oils have insecticidal activity against Plutella xylostella", 《INDUSTRIAL CROPS & PRODUCTS》 * |
| OLONISAKIN ADEBISI等: "Volatile, non-volatile composition and insecticidal activity of Eupatorium adenophorumSpreng against diamondback moth, Plutella xylostella (L.), and aphid, Aphis craccivora Koch", 《TOXIN REVIEWS》 * |
| R.H.BADGUJAR等: "Management of Plutella xylostella Using Cinnamomum zeylanicum and Syzygium aromaticum Extracts and their Major Secondary Metabolites", 《BIOPESTICIDES INTERNATIONAL》 * |
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