CN109738403A - A kind of preparation method of fluorescence standard card and fluorescence standard card fluorescent film - Google Patents
A kind of preparation method of fluorescence standard card and fluorescence standard card fluorescent film Download PDFInfo
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- CN109738403A CN109738403A CN201910004357.XA CN201910004357A CN109738403A CN 109738403 A CN109738403 A CN 109738403A CN 201910004357 A CN201910004357 A CN 201910004357A CN 109738403 A CN109738403 A CN 109738403A
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- container
- standard card
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- 238000002360 preparation method Methods 0.000 title claims description 11
- 239000000843 powder Substances 0.000 claims description 28
- 239000000725 suspension Substances 0.000 claims description 20
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims description 12
- 229920005573 silicon-containing polymer Polymers 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- -1 Oxygen alkane Chemical class 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 229910052761 rare earth metal Inorganic materials 0.000 claims description 5
- 150000002910 rare earth metals Chemical class 0.000 claims description 5
- 238000007711 solidification Methods 0.000 claims description 4
- 230000008023 solidification Effects 0.000 claims description 4
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims description 3
- 229910002651 NO3 Inorganic materials 0.000 claims description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 3
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 3
- 150000004645 aluminates Chemical class 0.000 claims description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 3
- 239000001506 calcium phosphate Substances 0.000 claims description 3
- 235000011010 calcium phosphates Nutrition 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- JZZIHCLFHIXETF-UHFFFAOYSA-N dimethylsilicon Chemical compound C[Si]C JZZIHCLFHIXETF-UHFFFAOYSA-N 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 230000036039 immunity Effects 0.000 abstract description 11
- 230000007774 longterm Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 229920000297 Rayon Polymers 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000011953 bioanalysis Methods 0.000 description 3
- 239000012780 transparent material Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004164 analytical calibration Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 150000002602 lanthanoids Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of fluorescence standard cards, including get stuck and container, it is described get stuck on be socketed with barn door, the side of the barn door is arranged in the container；The fitting of the side of the bottom outer wall of the container and the barn door；Several group windows are provided on the barn door；Fluorescent film is provided in the bottom interior wall of the container.Fluorescence standard card photoluminescent property of the invention is stable, simple and practical in structure, and fluorescence standard card can be realized the calibration to fluorescence immunity analyzer device, and can be steady in a long-term.
Description
Technical field
The invention belongs to instrument calibration field, in particular to the system of a kind of fluorescence standard card and fluorescence standard card fluorescent film
Preparation Method.
Background technique
Currently, the fluorescence immunity analyzer device based on fluorescent material generallys use certain wavelength when carrying out item detection
It is irradiated with the light source of intensity, so that the marker on fluorescent film issues fluorescence, then fluorescence signal is converted into electric signal and is drawn
At matched curve, the area of calculated curve wave crest or intensity determine the intensity of corresponding fluorescence signal, to set up telecommunications
Relationship number between sample concentration.
But during fluorescence-based fluorescence immunity analyzer device is determined fluorescence intensity, since fluorescence is exempted from
Sensor inside epidemic disease analysis instrument, is influenced by factors such as signal source strength, use environments and instrument during operation
In long-time use process, testing result is caused biggish deviation occur due to equipment breakage aging.Therefore, one is being carried out
Before a item detection, if lacking the calibration to fluorescence immunity analyzer device, will affect apparatus measures result consistency and
Accuracy.Currently, being based on phosphor not yet dedicated for fluorescence immunity analyzer device and in situ hybridization, biochip etc. are various
The fluorescence standard card of the bioanalysis detector calibration of material.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of fluorescence standard cards, including get stuck and container,
It is described get stuck on be socketed with barn door, the side of the barn door is arranged in the container；
The fitting of the side of the bottom outer wall of the container and the barn door；
Several group windows are provided on the barn door；
Fluorescent film is provided in the bottom interior wall of the container.
Further, the side wall of the barn door and the complete fitting of getting stuck, and another lateral plates of the barn door
It is concordant with the side plate face to get stuck to be arranged.
Further, the side got stuck is arranged in the container.
Further, the bottom outer wall of the container is fixedly connected with the side got stuck.
Further, the bottom of the container uses detachable snap fit, is fixedly connected on the side got stuck
It is interior.
Further, the window is provided at least two groups pair on the barn door through being socketed on the barn door
Claim the window of setting.
Further, the bottom outer wall of one end of the window and the container is bonded.
The present invention also provides a kind of preparation method of above-mentioned fluorescence standard card fluorescent film, the preparation method includes,
It weighs suitable fluorescent powder to be put into vial, dimethyl silicone polymer and poly- diformazan is separately added into vial
Radical siloxane curing agent carries out heating stirring to the mixture in vial, the first suspension is made after mixing evenly；
Dimethyl silicone polymer curing agent is added into the first suspension, mixture is stirred under room temperature environment,
The second suspension is made after mixing evenly；
The second suspension prepared is filled into container, the second suspension is by standing solidification, in container bottom
One layer of fluorescent film is formed on wall.
Further, after dimethyl silicone polymer and dimethyl silicone polymer curing agent are separately added into vial,
Under 50 DEG C of environment, mixture in vial is stirred, is stirred duration at least two hours.
Further, halogen calcium phosphate fluoressent powder, rare-earth trichromatic fluorescent powder, nitrate fluorescence can be selected in the fluorescent powder
One of powder, silicate fluorescent powder, borate fluorescent powder and aluminate fluorescent powder are a variety of.
Fluorescence standard card photoluminescent property of the invention is stable, simple and practical in structure, and fluorescence standard card can be realized to fluorescence
The calibration of immunoassay instrument, and can be steady in a long-term.
Other features and advantages of the present invention will be illustrated in the following description, also, partly becomes from specification
It obtains it is clear that understand through the implementation of the invention.The objectives and other advantages of the invention can be by specification, right
Pointed structure is achieved and obtained in claim and attached drawing.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without creative efforts, can be with root
Other attached drawings are obtained according to these attached drawings.
Fig. 1 shows the main view of fluorescence standard card according to an embodiment of the present invention；
Fig. 2 shows the front sectional view of fluorescence standard card according to a first embodiment of the present invention；
Fig. 3 shows the front sectional view of fluorescence standard card according to a second embodiment of the present invention；
Fig. 4 shows the top view of fluorescence standard card according to an embodiment of the present invention.
In figure: 1, getting stuck；2, container；3, barn door；4, fluorescent film.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical solution in the embodiment of the present invention clearly and completely illustrated, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
The present invention provides a kind of fluorescence standard cards, including get stuck 1 and container 2.
Described get stuck is socketed with barn door 3 on 1.Specifically, the side wall of the barn door 3 and 1 fitting completely of getting stuck,
And the side plate face of the barn door 3 concordant is arranged with the 1 side plate face of getting stuck.The container 2 is arranged in the shading
The side of plate 3.Specifically, the container 2 be arranged in it is described get stuck 1 side.Further, the bottom outer wall of the container 2
It can be fixedly connected with 1 side of getting stuck；The bottom of the container 2 can also be used detachable be connected to and described get stuck 1
Side in.
The bottom outer wall of the container 2 and the side fitting of the barn door 3；Several groups are provided on the barn door 3
Window 31.Specifically, the window 31 is provided at least two groups pair on the barn door 3 through being socketed on the barn door 3
Claim the window 31 of setting.
Fluorescent film 4 is provided in the bottom interior wall of the container 2.Further, one end of the window 31 and the appearance
The bottom outer wall of device 2 is bonded.
Fig. 1 shows the main view of fluorescence standard card according to an embodiment of the present invention, and fluorescence standard card includes get stuck 1 and appearance
Device 2, getting stuck 1 uses bar shaped plate structure, and getting stuck in the present embodiment 1 uses rectangle tabular structure, but get stuck 1 shape
Be not limited to rectangle, be also possible to square or other shapes, get stuck 1 concrete shape depend on the fluorescence standard card be used in
Detecting instrument sense channel structure.Plastics can be used or ceramics are made in 1 material of getting stuck.Further, the setting of container 2 exists
Get stuck 1 downside, container 2 is whole to use transparent material.Specifically, the bottom of container 2 is close to 1 side of getting stuck, the container of container 2
Mouthful far from getting stuck 1.Container 2 is for holding detection fluorescent film 4.Container 2 uses transparent material, is to ensure that light beam can penetrate
2 bottom of container, is radiated on fluorescent film 4, and the light that fluorescent film 4 reflects equally can be through the bottom of container 2, so holding
Device 2 can also be only limited to its bottom and use transparent material, and the side wall of container 2 can use opaque material.Further,
Container 2 relative to get stuck 1 position, depending on barn door 3 in the position on 1 of getting stuck.
Container 2 and getting stuck is fixedly connected between 1 using detachable snap fit.Illustratively, as shown in Fig. 2, getting stuck 1
On be provided with the rectangular through-hole through socket, shape of through holes is not limited to rectangle, is also possible to other shapes, such as round, pentagon.
The side of through-hole is socketed with barn door 3.Further, barn door 3 can pass through viscose glue and the bonding between 1 that gets stuck is solid in through-hole
It is fixed, barn door 3 can also using it is detachable be connected to get stuck 1 through-hole in.Specifically, the shape of barn door 3 and through-hole is big
It is small identical, and the side of barn door 3 concordant is arranged with 1 side of getting stuck.Further, barn door 3, which can be set, is getting stuck 1
Symmetrical center positions also can be set and get stuck on 1 close to the position at 1 edge that gets stuck, and barn door 3 takes in the position got stuck on 1
Certainly in the sense channel structure of its detecting instrument being used in.Further, the bottom of container 2 it is detachable be fixed on it is logical
In the other side in hole.Specifically, the bottom outer wall of container 2 and the side fitting of barn door 3, after avoiding light source from irradiating, due to glimmering
Light standard card plane of illumination is not concordant, and the light of reflection influences testing result.Further, fluorescent film 4 is contained in container 2,
Fluorescent film 4 is fixed in the bottom interior wall of container 2.Container 2 is whole to use rectangular parallelepiped structure, and the cross-sectional shape of container 2 is big
It is small identical with the cross-sectional shape size of through-hole.Container 2 is not limited to rectangular parallelepiped structure, the present embodiment by taking rectangular parallelepiped structure as an example into
Row exemplary illustration, container 2 is whole also to can be used cylindrical structure, guarantees 2 cross-sectional shape size of container and through-hole cross section
Shape size is consistent.To guarantee that detecting instrument verification is accurate, it is ensured that fluorescent film 4 is evenly distributed in the bottom of container 2
On wall.Container 2 and getting stuck is fixedly connected between 1 using detachable snap fit, convenient for being dismounted to fluorescence standard card, when
A certain parts damages on fluorescence standard card can carry out replacement use, save resource, improve the practical of fluorescence standard card
Property；And various sizes of component combination can be selected according to different detecting instruments, improve the applicable model of fluorescence standard card
It encloses.
It container 2 and gets stuck and integral type can also be used between 1 is fixedly connected.Illustratively, as shown in Fig. 2, it is close on container 2
The side wall of its bottom by viscose glue, be adhesively fixed on get stuck 1 through-hole in.It container 2 and gets stuck and can not also use clamping between 1
Mode connect.Illustratively, as shown in figure 3, getting stuck is socketed with barn door 3 in 1, specifically, the two sides of barn door 3 and getting stuck
1 two sides are respectively set concordantly, and after avoiding light source from irradiating, since fluorescence standard card plane of illumination is not concordant, the light of reflection influences
Testing result.Further, barn door 3 is rectangular plate-like structure, but barn door 3 is not limited to rectangular plate-like structure, barn door 3
Edge and the through-hole side wall to get stuck on 1 are bonded completely, it is ensured that the encapsulation of fluorescence standard card completely, ensure that through fluorescence standard card school
Test the accuracy of detecting instrument.Further, barn door 3 can be set get stuck 1 symmetrical center positions, also can be set
It gets stuck on 1 close to the position at 1 edge that gets stuck, barn door 3 depends on the inspection of its detecting instrument being used in the position on 1 of getting stuck
Survey channel design.Further, the side of barn door 3 is provided with container 2.Specifically, the bottom size of container 2 is than barn door 3
Plate face size it is big, and the symmetrical centre of 2 bottom outer wall of the plate face symmetrical centre of barn door 3 and container is to positive fitting.Into
One step, the bottom outer wall edge of container 2 are adhesively fixed by viscose glue and 1 side of getting stuck.Specifically, the bottom of container 2 and
The get stuck part of 1 fitting is adhesively fixed by viscose glue.Further, the generally rectangular parallelepiped structure of container 2, but the knot of container 2
Structure is not limited to cuboid, may be designed in other stereochemical structures.Further, fluorescent film 4, fluorescence are contained in container 2
Film 4 is fixed in the bottom interior wall of container 2.To guarantee that detecting instrument verification is accurate, it is ensured that fluorescent film 4 is evenly distributed on container
In 2 bottom interior wall.It container 2 and gets stuck between 1 using integral structure, simplifies the structure of fluorescence standard card, be suitable for referring to
Fixed detecting instrument uses, convenient for distinguishing management.
As shown in figure 4, being arranged there are two but being not limited to two windows 31 on barn door 3.Specifically, window 31 runs through
It is arranged on barn door 3, it is ensured that when verifying detection, detection light beam can be radiated at the fluorescent film in container 2 through window 31
On 31.Further, window 31 is rectangle structure, but 31 shape of window is not limited to rectangle, may be designed in other
Shape.
Fluorescence standard card favorable repeatability of the invention, accuracy are high, low in cost.It is equipped with the fluorescence of the fluorescence standard card
Immunoassay instrument testing result accuracy is high.
Fluorescence standard card of the invention is not limited to calibrate a certain fluorescence immunity analyzer device, it may also be used for calibration is in situ miscellaneous
The various bioanalysis detectors based on fluorescent material such as friendship, biochip, the present invention by taking fluorescence immunity analyzer device as an example into
Row exemplary illustration.Furthermore the fluorescence standard card may also be combined with time-resolved fluorescence detection, to ensure that each bioanalysis inspection
Survey the consistency and accuracy of the testing result of instrument.
The present invention also provides a kind of preparation methods of above-mentioned fluorescence standard card fluorescent film.Steps are as follows for preparation method:
Step 1: it weighs suitable fluorescent powder and is put into vial, dimethyl silicone polymer is separately added into vial
With dimethyl silicone polymer curing agent, heating stirring is carried out to mixture in vial, the first suspension is made after mixing evenly；
Step 2: into the first suspension be added dimethyl silicone polymer curing agent, under room temperature environment to mixture into
Row stirring, is made the second suspension after mixing evenly；
Step 3: the second suspension prepared is filled into container 2, and the second suspension is holding by standing solidification
One layer of fluorescent film 4 is formed in 2 bottom interior wall of device.
Above-mentioned rare earth inorganic fluorescent powder can be selected halogen calcium phosphate fluoressent powder, rare-earth trichromatic fluorescent powder, nitrate fluorescent powder,
One of silicate fluorescent powder, borate fluorescent powder and aluminate fluorescent powder are a variety of.Using glimmering made from above-mentioned fluorescent powder
Light film chemistry is stablized, and can be steady in a long-term, carries out school to fluorescence immunity analyzer device by using this fluorescence standard card
Standard can reduce the degree of deviation of the fluorescence immunity analyzer device testing result based on fluorescent material.Ensure fluorescence immunity analyzer
Device measures coefficient of variation CV and is less than or equal to 0.1%.
Illustratively, the method and step of fluorescent film 4 is specifically quantitatively prepared are as follows:
Step 1: weighing red lanthanide inorganic fluorescent powder 0.2g, and load weighted rare earth inorganic fluorescent powder is put into 3m l glass
In glass bottle, 0.1g dimethyl silicone polymer and 0.5m l chloroform are separately added into vial, to glass under 50 DEG C of environment
Mixture in glass bottle is stirred, and is stirred duration at least two hours, it is ensured that after mixing evenly, the first suspension is made；
Step 2: 0.1g dimethyl siloxane curing agent is added into the first suspension, to mixture under room temperature environment
It is stirred, is uniformly mixed it, the second suspension is made；
Step 3: the second suspension prepared is filled into container 2, and the second suspension is holding by standing solidification
The bottom interior wall of device 2 forms one layer of fluorescent film 4.
Utilize the process of fluorescence standard card of the invention and the functionality principle of fluorescence detection: the fluorescence standard that will be prepared
Card is put into the sense channel of detecting instrument, is made to detect light source face and is got stuck the window 31 of 1 and barn door 3, using certain wavelength
It is irradiated with the light source of intensity, light beam is radiated on the fluorescent film 4 in container 2, through the window 31 on barn door 3 so that fluorescence
Marker on film 4 issues fluorescence, and detecting instrument will capture fluorescence signal, and fluorescence signal is converted into electric signal, detector
Electric signal is depicted as matched curve by device, and the area of calculated curve wave crest or intensity determine the intensity of corresponding fluorescence signal,
To set up the relationship between electric signal and sample concentration.The fluorescent material that fluorescence standard card of the invention uses is dilute
Native inorganic fluorescent powder, is different from common fluorescent material and organic fluorescence materials, and the chemical property of itself ensure that the object
The long-term stability of matter can calibrate the fluorescence immunity analyzer device based on fluorescent material, thus lowering apparatus into
The degree of deviation in row item detection.
Although the present invention is described in detail referring to the foregoing embodiments, those skilled in the art should manage
Solution: it can still modify to the technical solution of foregoing embodiments, or be equal to part of technical characteristic
Replacement；And these are modified or replaceed, the technical solution for various embodiments of the present invention that it does not separate the essence of the corresponding technical solution
Spirit and scope.
Claims (10)
1. a kind of fluorescence standard card, including get stuck (1) and container (2), which is characterized in that
Described get stuck is socketed with barn door (3) on (1), side of container (2) setting in the barn door (3)；
The bottom outer wall of the container (2) and the side fitting of the barn door (3)；
Several group windows (31) are provided on the barn door (3)；
Fluorescent film (4) are provided in the bottom interior wall of the container (2).
2. fluorescence standard card according to claim 1, which is characterized in that the side wall of the barn door (3) and described get stuck
(1) fitting completely, and another lateral plates of the barn door (3) get stuck that the side plate face of (1) is concordant to be arranged with described.
3. fluorescence standard card according to claim 1, which is characterized in that the container (2) setting is in get stuck (1)
Side.
4. fluorescence standard card according to claim 3, which is characterized in that the bottom outer wall of the container (2) and the card
The side of shell (1) is fixedly connected.
5. fluorescence standard card according to claim 3, which is characterized in that the bottom of the container (2) is using detachable
Snap fit, be fixedly connected on it is described get stuck (1) side in.
6. fluorescence standard card according to claim 1, which is characterized in that the window (31), which is run through, is socketed in the shading
On plate (3), the symmetrically arranged window of at least two groups (31) is provided on the barn door (3).
7. fluorescence standard card according to claim 1, which is characterized in that one end of the window (31) and the container
(2) bottom outer wall fitting.
8. the preparation method of any fluorescence standard card fluorescent film in a kind of claim 1-7, which is characterized in that described
Preparation method includes,
It weighs suitable fluorescent powder to be put into vial, dimethyl silicone polymer and poly dimethyl silicon is separately added into vial
Oxygen alkane curing agent carries out heating stirring to the mixture in vial, the first suspension is made after mixing evenly；
Dimethyl silicone polymer curing agent is added into the first suspension, mixture is stirred under room temperature environment, stirs
Obtained second suspension after uniformly；
The second suspension prepared is filled into container (2), the second suspension is by standing solidification, in container (2) bottom
One layer of fluorescent film (4) is formed on inner wall.
9. preparation method according to claim 8, which is characterized in that be separately added into dimethyl silicone polymer into vial
After dimethyl silicone polymer curing agent, under 50 DEG C of environment, mixture in vial is stirred, stirring duration is at least
Two hours.
10. preparation method according to claim 8, which is characterized in that the fluorescent powder can be selected halogen calcium phosphate fluoressent powder,
One of rare-earth trichromatic fluorescent powder, nitrate fluorescent powder, silicate fluorescent powder, borate fluorescent powder and aluminate fluorescent powder
Or it is a variety of.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114966069A (en) * | 2022-05-25 | 2022-08-30 | 上海八通生物科技股份有限公司 | Quality control strip of fluorometer and preparation method thereof |
| CN115508560A (en) * | 2022-09-20 | 2022-12-23 | 上海市计量测试技术研究院 | A kind of calibration strip and its preparation method and application |
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Application publication date: 20190510 |