A kind of ecological system fertilizer of biologic ferment and the preparation method and application thereof
Technical field
The present invention relates to biologic ferment ecological fertilizers, belong to microbial fermentation and field of agricultural cultivation.
Background technique
In recent years, due to the deterioration of agroecological environment, the unreasonable use of agricultural input product, regulatory measure it is unsound
A series of problems, such as, cause Quality Safety Problems of Agricultural Products outstanding day by day, becomes focus concerned by people.Biologic ferment fertilizer was both
There are efficient inorganic nutrients, and have good organic nutrient, there are also the biopotency of multiple-effect, by the development of the fertilizer and makes
With, to alleviate from the angle of Safe fertilizer agricultural product security there are the problem of.
Biologic ferment fertilizer mainly utilizes microbial fermentation natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff,
And the appropriate microelement of coupling and growth stimulant are added, the fertilizer ecosystem an of entirety is formed, being applied to after soil can
Make biological, organic and inorganic mutual promotion, learn from other's strong points to offset one's weaknesses, playing a role to play jointly improves soil texture and environment, reduces disease
The use of insect pest generation and pesticide promotes crops healthy growth and obtains the agricultural product of safe and high quality.
Microorganism formulation refers to that " a kind of specific products containing viable microbial, is applied in agricultural production, crop can be obtained
Specific fertilizer effect is obtained, in the production of this effect, viable microbial or its metabolite in product play crucial work
With ", because microorganism formulation is that a kind of lived cell living is playing an important role, effect will receive the serious of environment
Influence, such as the influence of nutrient environment, soil environment, indigenous microorganism and weather, thus the promotion conference of microorganism formulation by
Very big influence, the present invention is based on these considerations, on the basis of fully considering microorganism formulation function, are entering soil
Microorganism is first given before environment builds suitable nutrient environment, such as organic active carrier, organic ferment nutrition, inorganic nutrients
And micro-element nutrition, the ecosystem for being configured to an Adaptable growth to microorganism enter around rhizosphere, breed rapidly,
Dominant bacteria is formed, to play the effect that real growth promotion proposes quality.
Summary of the invention
The purpose of the present invention is disclose a kind of ecological system fertilizer of biologic ferment and the preparation method and application thereof.It is special
Sign is that it is the filtered fluid being separately added into after function bacterium fermentation by natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff
Mixture carry out again ferment after be added natural minerals element, agricultural and chitosan oligosaccharide, agricultural grade microelement produces, be one
Kind integrate microorganism, organic active carrier, organic nutrition, micro-element nutrition the biologic ferment for adapting to microorganism and growing
Ecological system fertilizer, can breed rapidly after around rhizosphere, form dominant bacteria, so that real effect is played, including
Antibacterial insect prevention, anti-continuous cropping stimulate crop growth, improve the effect of utilization rate of fertilizer, crop yield and quality, it is under
State the fermentation material composition of parts by weight:
Natural organic matter material: 2~5 parts;Chinese medicine: 2~5 parts;Fruits and vegetables: 2~5 parts;Vegetable castoff: 2~5 parts;Water: 10
~20 parts;Sugar: 0.5~1.0 part;0.02~0.04 part of function bacterium;0.02~0.04 part of natural minerals element;Agricultural grade chitosan oligosaccharide
0.0001~0.0002 part;0.01~0.02 part of agricultural grade microelement.
Function bacterium provided by the invention, its active constituent include saccharomycete (Saccharomyces cerevisiae),
Lactic acid bacteria (Lactobacillus plantarum), bacillus subtilis (Bacillus subtilis), gel-shaped gemma bar
Bacterium (Bacillus mucilaginosus) and Trichoderma harzianum (Trichoderma harzianum).
The saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be
0.005~0.01 part: 0.005~0.01 part: 0.005~0.01 part: 0.005~0.01 part: 0.005~0.01 part.
The saccharomycete can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 20065;Institute
Stating lactic acid bacteria can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10171;The withered grass
Bacillus can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 11089;The glue peptone
Sample bacillus can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10013.The Kazakhstan
Thatch trichoderma can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 30371.
The natural organic matter material parts by weight refer to: dregs of beans: 1~2 part;Cotton dregs: 0.5~1 part;Wheat bran: 1
~2 parts;Ground phosphate rock: 0.5~1 part.
The Chinese medicine parts by weight refer to: Radix Astragali: 1~2 part;Azedarach: 0.5~1 part;Honeysuckle: 1~
2 parts;Dandelion: 0.5~2 part.
The fruits and vegetables parts by weight refer to: 0.5~1 part of sea-buckthorn;0.5~1 part of pears;0.5~1 part of grape;Tomato
0.5~1 part;0.5~1 part of potato.
The vegetable castoff parts by weight refer to: 1~2 part of cucumber waste;1~2 part of cauliflower waste;Circle is white
The waste of dish: 1~2 part.
The natural minerals element refers to ooze mine and object, and market is purchased.
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased.
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.002 part;Copper sulphate:
0.001~0.002 part;Zinc sulfate: 0.001~0.002 part;Manganese sulfate: 0.001~0.002 part;Molybdenum: 0.001~0.002 part;
Boron: 0.001~0.002 part.
The present invention further discloses the productions and application method of the ecological system fertilizer of biologic ferment, it is characterised in that presses
Following step carries out:
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 1~2 part;Cotton dregs: 0.5~1 part;Wheat bran: 1
~2 parts;Ground phosphate rock: 0.5~1 part;Water: 10~20 parts;Brown sugar: 1~2 part;0.005~0.01 part of function bacterium;The function bacterium
Each strain parts by weight are as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio
For 0.001~0.002 part: 0.001~0.002 part: 0.001~0.002 part: 0.001~0.002 part: 0.001~
0.002 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 1~2 part;Azedarach: 0.5~1 part;Honeysuckle: 1~
2 parts;Dandelion: 0.5~2 part;Water: 10~15 parts;Brown sugar: 1~2 part;0.002~0.01 part of function bacterium;The function bacterium is each
Strain parts by weight are as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be
0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002
Part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of sea-buckthorn;0.5~1 part of pears;0.5~1 part of grape;Tomato
0.5~1 part;0.5~1 part of potato;Water: 15~20 parts;Brown sugar: 1.5~2.5 parts;0.002~0.01 part of function bacterium;The function
Can each strain parts by weight of bacterium it is as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight
Amount is than being 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004
~0.002 part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of cucumber waste;1~2 part of cauliflower waste;Circle
The waste of Chinese cabbage: 1~2 part;Water: 10~15 parts;Brown sugar: 2~4 parts;0.002~0.01 part of function bacterium;The function bacterium is each
Strain parts by weight are as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be
0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002
Part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears
In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function
Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks;
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into and clean and sterilize
In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add
Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(8) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(9) mixed fermentation: mistake after natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively is filtered
Filtrate continues fermentation 1 month after mixing by 1:1:1:1;
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach
100g/L, microelement reach 50g/L;
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, ploughed deeply after being manured into soil into
Soil;It sprays, is sprayed on blade.
The present invention further discloses the ecological system fertilizer of the biologic ferment containing Chinese medicine and is increasing tomato yield,
Improve the application in terms of diseases prevention rate, the especially application in terms of improving tomato total reducing sugar.The biologic ferment of the invention is ecological
System fertilizer application is after tomato planting, and compared with check plot, amount of increase in production 9.1%, diseases prevention rate 33% can be significantly improved kind
The total reducing sugar of eggplant, increasing degree 12.3%.
The effect of Radix Astragali that the present invention uses, azedarach, honeysuckle, dandelion is as follows: Radix Astragali has enhancing plant part to exempt from
Epidemic disease function and wide antibacterial action;Azedarach has the function of desinsection;Honeysuckle has effects that sterilization;Dandelion is rich in
Vitamin, potassium, calcium, copper, magnesium and folic acid can provide nutrition abundant for crop.
Present invention mainly solves being difficult to growth and breeding after microbe application to soil, high spot reviews naturally have
Interaction between machine nutrient, inorganic nutrients, natural minerals nutrient and microorganism, Major Difficulties be microorganism, organic nutrition,
Inorganic nutrients, natural minerals nutrient cogeneration systems are built.
Product possessed by the ecological system liquid fertilizer of biologic ferment disclosed by the invention and application compared with prior art
Pole effect is:
(1) beneficial microbe is to be applied in the form of an organic ecological system into soil;
(2) growth and breeding ability is high after beneficial microbe is manured into soil, volume increase, the upgrading, disease resisting effect played to plant growth
Obviously.
(3) safety of fertilizer is increased, unharmful substance improves the safety of agricultural product.
(4) raw material sterilization is sterilized and is placed twice using 200ppm chlorine dioxide (purchasing in market) 5 hours intermittences
24 hours methods, it is at low cost, it is harmless, conducive to the progress of fermenting raw materials.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Embodiment 1: the production and application method of the ecological system fertilizer of biologic ferment
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 1 part;Cotton dregs: 0.5 part;Wheat bran: 1 part;Phosphorus ore
Powder: 1 part;Water: 15 parts;Brown sugar: 2 parts;0.005 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, cream
Sour bacterium, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.001 part: 0.001 part: 0.001 part:
0.001 part: 0.001 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 1 part;Azedarach: 0.5 part;Honeysuckle: 1 part;Dandelion: 1
Part;Water: 10 parts;Brown sugar: 1 part;0.004 part of function bacterium;Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid bacteria,
Bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0008 part: 0.0008 part: 0.0008 part:
0.0008 part: 0.0008 part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 0.5 part of sea-buckthorn;0.5 part of pears;0.5 part of grape;Tomato 0.5;Potato
0.5 part;Water: 15 parts;Brown sugar: 1.5 parts;0.002 part of function bacterium.Each strain weight fraction score of function bacterium is as follows: yeast
Bacterium, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0004 part: 0.0004 part:
0.0004 part: 0.0004 part: 0.0004 part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 1 part of cucumber waste;1 part of cauliflower waste;Cabbage
Waste: 1 part;Water: 10 parts;Brown sugar: 2 parts;0.002 part of function bacterium.Each strain weight fraction score of function bacterium is as follows: ferment
Female bacterium, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0004 part: 0.0004 part:
0.0004 part: 0.0004 part: 0.0004 part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears
In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function
Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks;
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into and clean and sterilize
In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add
Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(8) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(9) mixed fermentation: mistake after natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively is filtered
Filtrate continues fermentation 1 month after mixing by 1:1:1:1;
(10) natural minerals element is added: 0.01 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach
100g/L, microelement reach 50g/L;
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, ploughed deeply after being manured into soil into
Soil;It sprays, is sprayed on blade.
Embodiment 2: the production and application method of the ecological system fertilizer of biologic ferment
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 2 parts;Cotton dregs: 1 part;Wheat bran: 1 part;Ground phosphate rock:
0.5 part;Water: 20 parts;Brown sugar: 1 part;0.01 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid
Bacterium, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.002 part: 0.002 part: 0.002 part:
0.002 part: 0.002 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 2 parts;Azedarach: 1 part;Honeysuckle: 1 part;Dandelion: 0.5
Part;Water: 15 parts;Brown sugar: 2 parts;0.01 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid bacteria,
Bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.002 part: 0.002 part: 0.002 part: 0.002
Part: 0.002 part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 1 part of sea-buckthorn;1 part of pears;1 part of grape;Tomato 1;1 part of potato;Water: 15
Part;Brown sugar: 3 parts;0.01 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid bacteria, withered grass gemma
Bacillus, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.002 part: 0.002 part: 0.002 part: 0.002 part: 0.002
Part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 2 parts of cucumber waste;2 parts of cauliflower waste;Cabbage
Waste: 1 part;Water: 10 parts;Brown sugar: 2 parts;0.005 part of function bacterium.Each strain parts by weight of function bacterium are as follows: yeast
Bacterium, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.001 part: 0.001 part: 0.001
Part: 0.001 part: 0.001 part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears
In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function
Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks.
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into clean and disappears
Brown sugar is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in the white plastic barrel of poison
→ addition function fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month.
(7) it fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → is chosen is respectively put into and clean and sterilize
In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add
Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month.
(8) high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → difference vegetable castoff fermentation process: are chosen
Be put into clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and to place 24 small
When → addition brown sugar → addition function fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month.
(9) filter mixed fermentation: natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff ferment 1 month to be filtered respectively
Continue fermentation 1 month after filtered fluid is mixed by 1:1:1:1 afterwards.
(10) natural minerals element is added: 0.01 part of natural minerals element being added after filtering mixed fermentation.
(11) agricultural grade chitosan oligosaccharide is added: 0.0001 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation.
(12) agricultural grade microelement is added: 0.01 part of agricultural grade microelement being added after filtering mixed fermentation.
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach
100g/L, microelement reach 50g/L.
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, and ploughs deeply after being manured into soil
It buries;It sprays, is sprayed on blade.
Embodiment 3: the field trial of the ecological system fertilizer of biologic ferment
The ecological system fertilizer of biologic ferment can stimulate plant growth, bacteriostatic and disease prevention, and it is raw that nutrient, enhancing soil can be provided for crop
Object activity.
, test site and varieties of plant
Test site is Tianjin Wuqing chrysanthemum shop, and test variety is tomato.
, field design
Totally 2 processing, 3 repetitions of each processing, plot area 20m2。
Processing 1: the ecological system fertilizer of conventional fertilizer application+biologic ferment;Processing 2: control (conventional fertilizer application).
, test result
Application effect of the ecological system fertilizer of 1 biologic ferment of table in tomato planting
Test result shows that tomato increases the ecological system fertilizer of biologic ferment, compared with check plot, amount of increase in production 9.1%,
Diseases prevention rate 33%.From attributional analysis result it can be seen that increasing the ecological system fertilizer of biologic ferment, tomato can be significantly improved
Total reducing sugar, increasing degree 12.3%.