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CN109734524A - Biological enzyme ecological system fertilizer and preparation method and application thereof - Google Patents

Biological enzyme ecological system fertilizer and preparation method and application thereof Download PDF

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Publication number
CN109734524A
CN109734524A CN201910215891.5A CN201910215891A CN109734524A CN 109734524 A CN109734524 A CN 109734524A CN 201910215891 A CN201910215891 A CN 201910215891A CN 109734524 A CN109734524 A CN 109734524A
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parts
fermentation
weight
added
function
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何宗均
路垚
李峰
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Tianjin Academy of Agricultural Sciences
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TIANJIN INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT SCIENCE
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

本发明公开了一种生物酵素生态性系统肥料及其应用。其特征在于它是一种集微生物、有机活性载体、有机营养、微量元素于一体适应微生物生长的生物酵素生态性系统肥料,是由下述重量份数的物料组成:天然有机物料:2~5份;中药材:2~5份;果蔬:2~5份;蔬菜废弃物:2~5份;水:10~20份;糖:0.5~1.0份;功能菌0.02~0.04份;天然矿物元素0.02~0.04份;农用级壳寡糖0.0001~0.0002份;农用级微量元素0.01~0.02份。所述肥料稀释500倍喷施于叶面上,可为作物提供养分,促进作物生长、抑菌防病,应用于番茄种植后,比对照增产9.1%,防病率33%,番茄的总糖增加12.3%。The invention discloses a biological enzyme ecological system fertilizer and its application. It is characterized in that it is a biological enzyme ecological system fertilizer that integrates microorganisms, organic active carriers, organic nutrients and trace elements to adapt to the growth of microorganisms. It is composed of the following materials by weight: natural organic materials: 2-5 parts; Chinese medicinal materials: 2-5 parts; fruits and vegetables: 2-5 parts; vegetable waste: 2-5 parts; water: 10-20 parts; sugar: 0.5-1.0 parts; functional bacteria 0.02-0.04 parts; natural mineral elements 0.02~0.04 parts; agricultural grade chitosan oligosaccharide 0.0001~0.0002 parts; agricultural grade trace elements 0.01~0.02 parts. The fertilizer is diluted 500 times and sprayed on the foliage, which can provide nutrients for crops, promote crop growth, inhibit bacteria and prevent diseases. An increase of 12.3%.

Description

A kind of ecological system fertilizer of biologic ferment and the preparation method and application thereof
Technical field
The present invention relates to biologic ferment ecological fertilizers, belong to microbial fermentation and field of agricultural cultivation.
Background technique
In recent years, due to the deterioration of agroecological environment, the unreasonable use of agricultural input product, regulatory measure it is unsound A series of problems, such as, cause Quality Safety Problems of Agricultural Products outstanding day by day, becomes focus concerned by people.Biologic ferment fertilizer was both There are efficient inorganic nutrients, and have good organic nutrient, there are also the biopotency of multiple-effect, by the development of the fertilizer and makes With, to alleviate from the angle of Safe fertilizer agricultural product security there are the problem of.
Biologic ferment fertilizer mainly utilizes microbial fermentation natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff, And the appropriate microelement of coupling and growth stimulant are added, the fertilizer ecosystem an of entirety is formed, being applied to after soil can Make biological, organic and inorganic mutual promotion, learn from other's strong points to offset one's weaknesses, playing a role to play jointly improves soil texture and environment, reduces disease The use of insect pest generation and pesticide promotes crops healthy growth and obtains the agricultural product of safe and high quality.
Microorganism formulation refers to that " a kind of specific products containing viable microbial, is applied in agricultural production, crop can be obtained Specific fertilizer effect is obtained, in the production of this effect, viable microbial or its metabolite in product play crucial work With ", because microorganism formulation is that a kind of lived cell living is playing an important role, effect will receive the serious of environment Influence, such as the influence of nutrient environment, soil environment, indigenous microorganism and weather, thus the promotion conference of microorganism formulation by Very big influence, the present invention is based on these considerations, on the basis of fully considering microorganism formulation function, are entering soil Microorganism is first given before environment builds suitable nutrient environment, such as organic active carrier, organic ferment nutrition, inorganic nutrients And micro-element nutrition, the ecosystem for being configured to an Adaptable growth to microorganism enter around rhizosphere, breed rapidly, Dominant bacteria is formed, to play the effect that real growth promotion proposes quality.
Summary of the invention
The purpose of the present invention is disclose a kind of ecological system fertilizer of biologic ferment and the preparation method and application thereof.It is special Sign is that it is the filtered fluid being separately added into after function bacterium fermentation by natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff Mixture carry out again ferment after be added natural minerals element, agricultural and chitosan oligosaccharide, agricultural grade microelement produces, be one Kind integrate microorganism, organic active carrier, organic nutrition, micro-element nutrition the biologic ferment for adapting to microorganism and growing Ecological system fertilizer, can breed rapidly after around rhizosphere, form dominant bacteria, so that real effect is played, including Antibacterial insect prevention, anti-continuous cropping stimulate crop growth, improve the effect of utilization rate of fertilizer, crop yield and quality, it is under State the fermentation material composition of parts by weight:
Natural organic matter material: 2~5 parts;Chinese medicine: 2~5 parts;Fruits and vegetables: 2~5 parts;Vegetable castoff: 2~5 parts;Water: 10 ~20 parts;Sugar: 0.5~1.0 part;0.02~0.04 part of function bacterium;0.02~0.04 part of natural minerals element;Agricultural grade chitosan oligosaccharide 0.0001~0.0002 part;0.01~0.02 part of agricultural grade microelement.
Function bacterium provided by the invention, its active constituent include saccharomycete (Saccharomyces cerevisiae), Lactic acid bacteria (Lactobacillus plantarum), bacillus subtilis (Bacillus subtilis), gel-shaped gemma bar Bacterium (Bacillus mucilaginosus) and Trichoderma harzianum (Trichoderma harzianum).
The saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.005~0.01 part: 0.005~0.01 part: 0.005~0.01 part: 0.005~0.01 part: 0.005~0.01 part.
The saccharomycete can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 20065;Institute Stating lactic acid bacteria can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10171;The withered grass Bacillus can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 11089;The glue peptone Sample bacillus can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10013.The Kazakhstan Thatch trichoderma can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 30371.
The natural organic matter material parts by weight refer to: dregs of beans: 1~2 part;Cotton dregs: 0.5~1 part;Wheat bran: 1 ~2 parts;Ground phosphate rock: 0.5~1 part.
The Chinese medicine parts by weight refer to: Radix Astragali: 1~2 part;Azedarach: 0.5~1 part;Honeysuckle: 1~ 2 parts;Dandelion: 0.5~2 part.
The fruits and vegetables parts by weight refer to: 0.5~1 part of sea-buckthorn;0.5~1 part of pears;0.5~1 part of grape;Tomato 0.5~1 part;0.5~1 part of potato.
The vegetable castoff parts by weight refer to: 1~2 part of cucumber waste;1~2 part of cauliflower waste;Circle is white The waste of dish: 1~2 part.
The natural minerals element refers to ooze mine and object, and market is purchased.
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased.
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.002 part;Copper sulphate: 0.001~0.002 part;Zinc sulfate: 0.001~0.002 part;Manganese sulfate: 0.001~0.002 part;Molybdenum: 0.001~0.002 part; Boron: 0.001~0.002 part.
The present invention further discloses the productions and application method of the ecological system fertilizer of biologic ferment, it is characterised in that presses Following step carries out:
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 1~2 part;Cotton dregs: 0.5~1 part;Wheat bran: 1 ~2 parts;Ground phosphate rock: 0.5~1 part;Water: 10~20 parts;Brown sugar: 1~2 part;0.005~0.01 part of function bacterium;The function bacterium Each strain parts by weight are as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio For 0.001~0.002 part: 0.001~0.002 part: 0.001~0.002 part: 0.001~0.002 part: 0.001~ 0.002 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 1~2 part;Azedarach: 0.5~1 part;Honeysuckle: 1~ 2 parts;Dandelion: 0.5~2 part;Water: 10~15 parts;Brown sugar: 1~2 part;0.002~0.01 part of function bacterium;The function bacterium is each Strain parts by weight are as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 Part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of sea-buckthorn;0.5~1 part of pears;0.5~1 part of grape;Tomato 0.5~1 part;0.5~1 part of potato;Water: 15~20 parts;Brown sugar: 1.5~2.5 parts;0.002~0.01 part of function bacterium;The function Can each strain parts by weight of bacterium it is as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight Amount is than being 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004 ~0.002 part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of cucumber waste;1~2 part of cauliflower waste;Circle The waste of Chinese cabbage: 1~2 part;Water: 10~15 parts;Brown sugar: 2~4 parts;0.002~0.01 part of function bacterium;The function bacterium is each Strain parts by weight are as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 Part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks;
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into and clean and sterilize In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(8) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours → Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(9) mixed fermentation: mistake after natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively is filtered Filtrate continues fermentation 1 month after mixing by 1:1:1:1;
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach 100g/L, microelement reach 50g/L;
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, ploughed deeply after being manured into soil into Soil;It sprays, is sprayed on blade.
The present invention further discloses the ecological system fertilizer of the biologic ferment containing Chinese medicine and is increasing tomato yield, Improve the application in terms of diseases prevention rate, the especially application in terms of improving tomato total reducing sugar.The biologic ferment of the invention is ecological System fertilizer application is after tomato planting, and compared with check plot, amount of increase in production 9.1%, diseases prevention rate 33% can be significantly improved kind The total reducing sugar of eggplant, increasing degree 12.3%.
The effect of Radix Astragali that the present invention uses, azedarach, honeysuckle, dandelion is as follows: Radix Astragali has enhancing plant part to exempt from Epidemic disease function and wide antibacterial action;Azedarach has the function of desinsection;Honeysuckle has effects that sterilization;Dandelion is rich in Vitamin, potassium, calcium, copper, magnesium and folic acid can provide nutrition abundant for crop.
Present invention mainly solves being difficult to growth and breeding after microbe application to soil, high spot reviews naturally have Interaction between machine nutrient, inorganic nutrients, natural minerals nutrient and microorganism, Major Difficulties be microorganism, organic nutrition, Inorganic nutrients, natural minerals nutrient cogeneration systems are built.
Product possessed by the ecological system liquid fertilizer of biologic ferment disclosed by the invention and application compared with prior art Pole effect is:
(1) beneficial microbe is to be applied in the form of an organic ecological system into soil;
(2) growth and breeding ability is high after beneficial microbe is manured into soil, volume increase, the upgrading, disease resisting effect played to plant growth Obviously.
(3) safety of fertilizer is increased, unharmful substance improves the safety of agricultural product.
(4) raw material sterilization is sterilized and is placed twice using 200ppm chlorine dioxide (purchasing in market) 5 hours intermittences 24 hours methods, it is at low cost, it is harmless, conducive to the progress of fermenting raw materials.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even Mean value.
Embodiment 1: the production and application method of the ecological system fertilizer of biologic ferment
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 1 part;Cotton dregs: 0.5 part;Wheat bran: 1 part;Phosphorus ore Powder: 1 part;Water: 15 parts;Brown sugar: 2 parts;0.005 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, cream Sour bacterium, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.001 part: 0.001 part: 0.001 part: 0.001 part: 0.001 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 1 part;Azedarach: 0.5 part;Honeysuckle: 1 part;Dandelion: 1 Part;Water: 10 parts;Brown sugar: 1 part;0.004 part of function bacterium;Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid bacteria, Bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0008 part: 0.0008 part: 0.0008 part: 0.0008 part: 0.0008 part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 0.5 part of sea-buckthorn;0.5 part of pears;0.5 part of grape;Tomato 0.5;Potato 0.5 part;Water: 15 parts;Brown sugar: 1.5 parts;0.002 part of function bacterium.Each strain weight fraction score of function bacterium is as follows: yeast Bacterium, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0004 part: 0.0004 part: 0.0004 part: 0.0004 part: 0.0004 part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 1 part of cucumber waste;1 part of cauliflower waste;Cabbage Waste: 1 part;Water: 10 parts;Brown sugar: 2 parts;0.002 part of function bacterium.Each strain weight fraction score of function bacterium is as follows: ferment Female bacterium, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.0004 part: 0.0004 part: 0.0004 part: 0.0004 part: 0.0004 part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks;
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into and clean and sterilize In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(8) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours → Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(9) mixed fermentation: mistake after natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively is filtered Filtrate continues fermentation 1 month after mixing by 1:1:1:1;
(10) natural minerals element is added: 0.01 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach 100g/L, microelement reach 50g/L;
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, ploughed deeply after being manured into soil into Soil;It sprays, is sprayed on blade.
Embodiment 2: the production and application method of the ecological system fertilizer of biologic ferment
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 2 parts;Cotton dregs: 1 part;Wheat bran: 1 part;Ground phosphate rock: 0.5 part;Water: 20 parts;Brown sugar: 1 part;0.01 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid Bacterium, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.002 part: 0.002 part: 0.002 part: 0.002 part: 0.002 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 2 parts;Azedarach: 1 part;Honeysuckle: 1 part;Dandelion: 0.5 Part;Water: 15 parts;Brown sugar: 2 parts;0.01 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid bacteria, Bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.002 part: 0.002 part: 0.002 part: 0.002 Part: 0.002 part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 1 part of sea-buckthorn;1 part of pears;1 part of grape;Tomato 1;1 part of potato;Water: 15 Part;Brown sugar: 3 parts;0.01 part of function bacterium.Each strain parts by weight of function bacterium are as follows: saccharomycete, lactic acid bacteria, withered grass gemma Bacillus, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.002 part: 0.002 part: 0.002 part: 0.002 part: 0.002 Part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 2 parts of cucumber waste;2 parts of cauliflower waste;Cabbage Waste: 1 part;Water: 10 parts;Brown sugar: 2 parts;0.005 part of function bacterium.Each strain parts by weight of function bacterium are as follows: yeast Bacterium, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight ratio be 0.001 part: 0.001 part: 0.001 Part: 0.001 part: 0.001 part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks.
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into clean and disappears Brown sugar is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in the white plastic barrel of poison → addition function fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month.
(7) it fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → is chosen is respectively put into and clean and sterilize In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month.
(8) high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → difference vegetable castoff fermentation process: are chosen Be put into clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and to place 24 small When → addition brown sugar → addition function fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month.
(9) filter mixed fermentation: natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff ferment 1 month to be filtered respectively Continue fermentation 1 month after filtered fluid is mixed by 1:1:1:1 afterwards.
(10) natural minerals element is added: 0.01 part of natural minerals element being added after filtering mixed fermentation.
(11) agricultural grade chitosan oligosaccharide is added: 0.0001 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation.
(12) agricultural grade microelement is added: 0.01 part of agricultural grade microelement being added after filtering mixed fermentation.
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach 100g/L, microelement reach 50g/L.
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, and ploughs deeply after being manured into soil It buries;It sprays, is sprayed on blade.
Embodiment 3: the field trial of the ecological system fertilizer of biologic ferment
The ecological system fertilizer of biologic ferment can stimulate plant growth, bacteriostatic and disease prevention, and it is raw that nutrient, enhancing soil can be provided for crop Object activity.
, test site and varieties of plant
Test site is Tianjin Wuqing chrysanthemum shop, and test variety is tomato.
, field design
Totally 2 processing, 3 repetitions of each processing, plot area 20m2
Processing 1: the ecological system fertilizer of conventional fertilizer application+biologic ferment;Processing 2: control (conventional fertilizer application).
, test result
Application effect of the ecological system fertilizer of 1 biologic ferment of table in tomato planting
Test result shows that tomato increases the ecological system fertilizer of biologic ferment, compared with check plot, amount of increase in production 9.1%, Diseases prevention rate 33%.From attributional analysis result it can be seen that increasing the ecological system fertilizer of biologic ferment, tomato can be significantly improved Total reducing sugar, increasing degree 12.3%.

Claims (4)

1. a kind of ecological system fertilizer of biologic ferment, it is characterised in that it is made of the raw material of following portions by weight:
Natural organic matter material: 2~5 parts;Chinese medicine: 2~5 parts;Fruits and vegetables: 2~5 parts;Vegetable castoff: 2~5 parts;Water: 10 ~20 parts;Sugar: 0.5~1.0 part;0.02~0.04 part of function bacterium;0.02~0.04 part of natural minerals element;Agricultural grade chitosan oligosaccharide 0.0001~0.0002 part;0.01~0.02 part of agricultural grade microelement;
The parts by weight of the function bacterium refer to: saccharomycete: 0.005~0.01 part;Lactic acid bacteria: 0.005~0.01 part;Withered grass Bacillus: 0.005~0.01 part;Bacillusmusilaginosiengineering: 0.005~0.01 part;Kazakhstan thatch rasp berry: 0.005~0.01 part;
The natural organic matter material parts by weight refer to: dregs of beans: 1~2 part;Cotton dregs: 0.5~1 part;Wheat bran: 1~2 Part;Ground phosphate rock: 0.5~1 part;
The Chinese medicine parts by weight refer to: Radix Astragali: 1~2 part;Azedarach: 0.5~1 part;Honeysuckle: 1~2 part; Dandelion: 0.5~2 part;
The fruits and vegetables parts by weight refer to: 0.5~1 part of sea-buckthorn;0.5~1 part of pears;0.5~1 part of grape;Tomato 0.5~ 1 part;0.5~1 part of potato;
The vegetable castoff parts by weight refer to: 1~2 part of cucumber waste;1~2 part of cauliflower waste;Cabbage Waste: 1~2 part;
The natural minerals element refers to ooze mine and object, and market is purchased;
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased;
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.002 part;Copper sulphate: 0.001~ 0.002 part;Zinc sulfate: 0.001~0.002 part;Manganese sulfate: 0.001~0.002 part;Molybdenum: 0.001~0.002 part;Boron: 0.001~0.002 part, market is purchased.
2. the production and application method of the ecological system fertilizer of biologic ferment described in claim 1, it is characterised in that by following Step carries out:
(1) natural organic matter material biologic ferment fermentation raw material and formula: dregs of beans: 1~2 part;Cotton dregs: 0.5~1 part;Wheat bran: 1 ~2 parts;Ground phosphate rock: 0.5~1 part;Water: 10~20 parts;Brown sugar: 1~2 part;0.005~0.01 part of function bacterium;The function bacterium Each strain weight fraction score is as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight Amount is than being 0.001~0.002 part: 0.001~0.002 part: 0.001~0.002 part: 0.001~0.002 part: 0.001 ~0.002 part;
(2) Chinese medicine biologic ferment fermentation raw material and formula: Radix Astragali: 1~2 part;Azedarach: 0.5~1 part;Honeysuckle: 1~ 2 parts;Dandelion: 0.5~2 part;Water: 10~15 parts;Brown sugar: 1~2 part;0.002~0.01 part of function bacterium;The function bacterium is each Strain weight fraction score is as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight Than for 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~ 0.002 part;
(3) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of sea-buckthorn;0.5~1 part of pears;0.5~1 part of grape;Tomato 0.5~1 part;0.5~1 part of potato;Water: 15~20 parts;Brown sugar: 1.5~2.5 parts;0.002~0.01 part of function bacterium;The function Energy each strain weight fraction score of bacterium is as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum Weight ratio be 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part;
(4) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of cucumber waste;1~2 part of cauliflower waste;Circle The waste of Chinese cabbage: 1~2 part;Water: 10~15 parts;Brown sugar: 2~4 parts;0.002~0.01 part of function bacterium;The function bacterium is each Strain weight fraction score is as follows: saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Trichoderma harzianum weight Than for 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~0.002 part: 0.0004~ 0.002 part;
(5) it natural organic matter material fermentation process: chooses high-quality natural organic matter material → mixing → and is respectively put into clean and disappears In the white plastic barrel of poison → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes twice and place 24 hours → addition function Fermenting microbe → lid lid sealing individually fermentation, fermentation time two weeks;
(6) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → clean, be cut into segment → and is respectively put into and clean and sterilize In white plastic barrel → clear water → 200ppm chlorine dioxide 5 hours is intermittent sterilizes and place 24 hours → brown sugar is added → twice and add Function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized In charging basket → clear water → addition brown sugar → addition function fermenting microbe → lid lid sealing individually fermentation sterilized, fermentation time is added 1 month;
(8) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours → Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(9) mixed fermentation: mistake after natural organic matter material, Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively is filtered Filtrate continues fermentation 1 month after mixing by 1:1:1:1;
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 100U/L, polysaccharide 20g/L, amino acid reach 100g/ L, microelement reach 50g/L;
(14) application method: the dosage of 500 times of 5 kilograms of dilution imposes in soil per acre, and base is applied, ploughed deeply after being manured into soil into Soil;It sprays, is sprayed on blade.
3. biologic ferment fertilizer described in claim 1 is increasing tomato planting yield, the application in terms of tomato diseases prevention rate is improved.
4. application of the biologic ferment fertilizer described in claim 1 in terms of improving the total sugar amount of tomato.
CN201910215891.5A 2019-03-21 2019-03-21 Biological enzyme ecological system fertilizer and preparation method and application thereof Pending CN109734524A (en)

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CN110326630A (en) * 2019-07-18 2019-10-15 渭南职业技术学院 A method of biological pesticide is prepared using tomato stem-leaf
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CN111499433A (en) * 2020-04-21 2020-08-07 内蒙古风泽中孚生物科技有限公司 Multifunctional enzyme bio-organic fertilizer and preparation method thereof
CN113135778A (en) * 2021-03-03 2021-07-20 北京福缘地科技有限公司 Agricultural plant enzyme for preparing compost on site in field by utilizing agricultural organic wastes
CN113582779A (en) * 2021-07-30 2021-11-02 广西壮族自治区农业科学院 Method for planting vegetables by using sugarcane ash and fruit and vegetable wastes

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