CN109706079B - Flow cavity for simulating flowing environment behind bracket - Google Patents
Flow cavity for simulating flowing environment behind bracket Download PDFInfo
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- CN109706079B CN109706079B CN201811509283.7A CN201811509283A CN109706079B CN 109706079 B CN109706079 B CN 109706079B CN 201811509283 A CN201811509283 A CN 201811509283A CN 109706079 B CN109706079 B CN 109706079B
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Abstract
本发明为一种用于模拟支架后流动环境的流动腔，其特征在于：包括外螺杆，所述外螺杆中间设有滑动腔，所述滑动腔内转动设有滑杆，所述滑杆一端设有支架凸起，所述外螺杆靠近支架凸起的一端设有流动腔，所述流动腔中间设有凹槽，所述凹槽内设有载玻片，所述滑杆穿过所述滑动腔位于所述流动腔内。本发明实现对支架植入宿主血管后血流环境的体外模拟，为流动腔底部的培养细胞提供血流扰动的力学刺激。该发明中腔内高度可调和支架丝凸起高度可调使得本流动腔更加灵活地模拟支架入宿主血管后的多种力学环境；通过流动腔底部的可定量观察在该力学环境下培养细胞较为真实的生物学响应，从而为研究支架术后再狭窄和血栓等不良现象提供新的工具。
The present invention is a flow cavity for simulating the flow environment behind a bracket, which is characterized in that it includes an outer screw, a sliding cavity is arranged in the middle of the outer screw, and a sliding rod is rotated in the sliding cavity, and one end of the sliding rod is arranged. A bracket protrusion is provided, the end of the outer screw rod close to the bracket protrusion is provided with a flow cavity, a groove is arranged in the middle of the flow cavity, a glass slide is arranged in the groove, and the sliding rod passes through the A sliding cavity is located within the flow cavity. The invention realizes the in vitro simulation of the blood flow environment after the stent is implanted into the host blood vessel, and provides the mechanical stimulation of blood flow disturbance for the cultured cells at the bottom of the flow cavity. In the invention, the height of the inner cavity and the height of the protrusion of the stent wire are adjustable, so that the flow cavity can more flexibly simulate various mechanical environments after the stent is inserted into the host blood vessel; through the quantitative observation at the bottom of the flow cavity, the cultured cells in this mechanical environment are relatively Real biological responses, thus providing new tools for the study of adverse phenomena such as restenosis and thrombosis after stenting.
Description
Technical Field
The invention relates to a flow cavity for simulating a flowing environment behind a bracket, which is used for a biomedical experimental device, in particular to a flow cavity for a biological fluid experiment.
Background
Interventional stents are a very common and highly effective treatment for occlusive atherosclerotic conditions. Although the vascular stent has been greatly developed in the aspects of the configuration, the material, the placement process, the postoperative antithrombotic drug treatment and the like, the higher postoperative incidence of the intravascular restenosis and the late thrombus of the treatment method still influences the treatment effect. Studies have found that changes in the hemodynamic environment are closely related to the occurrence of adverse stenting events (restenosis, thrombosis, etc.).
Flow lumens are very effective tools for simulating in vivo flow environments, however, existing flat plate flow lumens are not sufficient to simulate post-stenting mechanical environments. The invention aims at blood vessels with different diameters and stents with different thicknesses, and provides a novel flow cavity for simulating a flow environment after stent operation.
Disclosure of Invention
The invention provides a flow cavity for simulating a flow environment after a stent is implanted, which comprises an outer screw rod, wherein the outer screw rod is provided with a sliding cavity, a sliding rod is arranged in the sliding cavity in a sliding manner, a stent bulge is arranged at one end of the sliding rod, the end, close to the stent bulge, of the outer screw rod is provided with the flow cavity, the flow cavity is provided with a groove for placing a glass slide, the sliding rod penetrates through the sliding cavity and is positioned in the flow cavity, and an inlet and an outlet are symmetrically arranged at two sides of the flow cavity.
Preferably, the support protrusion is composed of a plurality of support strips and a base body, the support strips are uniformly fixed at one end of the base body, and the other end of the base body is fixed on the sliding rod. The support bulge applies pressure to liquid in the flow cavity to simulate different liquid environments.
Further, one end of the outer screw rod, which is close to the bracket bulge, is connected with the shell of the flow cavity through threads. The flowing cavity can rotate through threads, and the height in the flowing cavity is adjusted, so that different experimental environments are achieved.
Preferably, the sliding rod is provided with a rubber ring, and the rubber ring slides in a piston manner with the sliding cavity. The rubber ring plays a sealing role, prevents that the liquid in the flow chamber flows out, simultaneously, the rubber ring is fixed on the slide bar, the slide bar can slide from top to bottom.
Furthermore, the outer screw is kept away from the one end in flow chamber is equipped with the end cover, the end cover passes through the fix with screw on the outer screw, be equipped with on the end cover be used for with the hole that slides the chamber intercommunication, the slide bar passes the hole slides and sets up in the slip intracavity.
Preferably, one end, far away from the support protrusion, of the sliding rod is provided with a limiting block, and the diameter of the limiting block is larger than that of the hole. The limiting block prevents the sliding rod from sliding to damage bacteria on the glass slide.
Further, the flowing cavity is made of transparent plexiglas material.
Preferably, the bracket projection is made of stainless steel or magnesium alloy.
Has the advantages that: the invention realizes the in vitro simulation of the blood flow environment after the stent is implanted into the host blood vessel and provides the mechanical stimulation of blood flow disturbance for the cultured cells at the bottom of the flow cavity. The height of the inner cavity of the flow cavity is adjustable, and the height of the protrusions of the stent wire is adjustable, so that the flow cavity can more flexibly simulate various mechanical environments after the stent is placed into a host blood vessel; the real biological response of the cultured cells in the mechanical environment (after the stent is implanted) can be quantitatively observed through the bottom of the flow cavity, so that a new tool is provided for researching adverse phenomena such as stent postoperative restenosis, thrombus and the like.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a slide bar structure according to the present invention;
FIG. 3 is a schematic view of a flow chamber configuration of the present invention;
FIG. 4 is a schematic view of the construction of the outer screw according to the present invention;
1. a flow chamber; 2. an outer screw rod; 3. a rubber ring; 4. the bracket is raised; 5. a slide bar; 6. an end cap; 7. a groove; 8. a limiting block; 9. a sliding cavity; 10. an inlet; 11. an outlet; 12. a substrate; 13. a support strip.
Detailed Description
As shown in fig. 1, a flow chamber 1 for simulating a flow environment behind a stent is characterized in that: including outer screw rod 2, be equipped with slip chamber 9 on outer screw rod 2, it is equipped with slide bar 5 to slide in the slip chamber 9, 5 one end of slide bar are equipped with support arch 4, support arch 4 is made by stainless steel or magnesium alloy, support arch 4 comprises a plurality of support strips 13 and base member 12, and is a plurality of support strip 13 evenly fixes the one end of base member 12, the base member 12 other end is fixed on slide bar 12. The support protrusions 4 apply pressure to the liquid in the flow cavity 1, and different liquid environments are simulated.
The one end that outer screw rod 2 is close to support arch 4 pass through the screw thread with the shell in flow chamber 1 is connected, flow chamber 1 is transparent plexiglas material, flow chamber 1 can rotate through the screw thread, adjusts the height in the flow chamber 1 to reach different experimental environment. A groove 7 for placing a glass slide is arranged in the middle of the flow cavity 1, and the slide bar 5 penetrates through the slide cavity 9 and is positioned in the flow cavity 1. The sliding rod 5 is provided with a rubber ring 3, and the rubber ring 3 is in sliding connection with the sliding cavity 9. The rubber ring 3 plays a sealing role, so that liquid in the flow cavity 1 is prevented from flowing out, meanwhile, the rubber ring 3 is fixed on the sliding rod 5, and the sliding rod 5 can slide up and down. The outer screw rod 2 is kept away from the one end in flow chamber 1 is equipped with end cover 6, end cover 6 passes through the fix with screw outer screw rod 2 is last, be equipped with on end cover 6 be used for with the hole that slides chamber 9 intercommunication, slide bar 5 passes the hole slides and sets up in sliding chamber 9. The flow chamber 1 is symmetrically provided with an inlet 10 and an outlet 11 at two sides. The slide bar 5 is kept away from the one end of support arch 4 is equipped with stopper 8, stopper 8 diameter is greater than the diameter in hole. The stop block 8 prevents the slide bar 5 from sliding beyond the stroke, thereby destroying bacteria on the slide.
The specific mode is as follows: when the device is used for an experiment, firstly the slide rod 5 is adjusted to enable the stent bulge 4 to reach the flow cavity 1 to meet the requirements of the experiment, then the cell slide glass with endothelial cells is arranged in the groove at the bottom of the flow cavity 1, then the outer screw rod 2 is adjusted to enable the height in the flow cavity 1 to be the same as that of a host blood vessel after stent operation, the flow cavity 1 is connected into a perfusion loop, and the growth state of the endothelial cells is observed through an inverted microscope.
Claims (7)
1. A flow chamber for simulating a post-stent flow environment, comprising: the glass slide device comprises an outer screw rod (2), wherein a sliding cavity (9) is formed in the outer screw rod (2), a sliding rod (5) is arranged in the sliding cavity (9) in a sliding mode, a support protrusion (4) is arranged at one end of the sliding rod (5), a flowing cavity (1) is formed in one end, close to the support protrusion (4), of the outer screw rod (2), a groove (7) used for placing a glass slide is formed in the flowing cavity (1), the sliding rod (5) penetrates through the sliding cavity (9) and is located in the flowing cavity (1), and an inlet (10) and an outlet (11) are symmetrically formed in two sides of the flowing cavity (1);
the support bulge (4) is composed of a plurality of support strips (13) and a base body (12), the support strips (13) are uniformly fixed at one end of the base body (12), and the other end of the base body (12) is fixed on the sliding rod (5).
2. A flow chamber for simulating a post-stent flow environment as recited in claim 1, wherein: one end of the outer screw rod (2) close to the support bulge (4) is connected with the shell of the flow cavity (1) through threads.
3. A flow chamber for simulating a post-stent flow environment as recited in claim 1, wherein: the rubber ring (3) is arranged on the sliding rod (5), and the rubber ring (3) is connected with the sliding cavity (9) in a sliding mode.
4. A flow chamber for simulating a post-stent flow environment as recited in claim 1, wherein: outer screw rod (2) are kept away from the one end in flow chamber (1) is equipped with end cover (6), end cover (6) pass through the fix with screw on outer screw rod (2), be equipped with on end cover (6) be used for with the hole of slip chamber (9) intercommunication, slide bar (5) pass the hole slides and sets up in slip chamber (9).
5. A flow chamber for simulating a post-stent flow environment as claimed in claim 4 wherein: the sliding rod (5) is far away from one end of the support protrusion (4) and is provided with a limiting block (8), and the diameter of the limiting block (8) is larger than that of the hole.
6. A flow chamber for simulating a post-stent flow environment as recited in claim 1, wherein: the flowing cavity (1) is made of transparent resin glass material.
7. A flow chamber for simulating a post-stent flow environment as recited in claim 1, wherein: the support protrusion (4) is made of stainless steel or magnesium alloy.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811509283.7A CN109706079B (en) | 2018-12-11 | 2018-12-11 | Flow cavity for simulating flowing environment behind bracket |
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| CN201811509283.7A CN109706079B (en) | 2018-12-11 | 2018-12-11 | Flow cavity for simulating flowing environment behind bracket |
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| CN109706079A CN109706079A (en) | 2019-05-03 |
| CN109706079B true CN109706079B (en) | 2022-03-25 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110675717B (en) * | 2019-10-10 | 2021-09-14 | 吉林大学 | Bionic equipment for simulating vascular stenosis and thrombus |
| CN113503907B (en) * | 2021-08-11 | 2022-09-27 | 清华大学 | Extracorporeal circulation experimental bench and experimental method for medical implant intervention observation |
| CN113503906B (en) * | 2021-08-11 | 2022-12-20 | 清华大学 | In-vitro circulation experiment table and method for medical implant intervention body thrombosis |
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| CN201217661Y (en) * | 2008-03-28 | 2009-04-08 | 天津理工大学 | A bioreactor for dual-frequency loading of artificial cartilage |
| CN201817494U (en) * | 2010-08-10 | 2011-05-04 | 中国人民解放军军事医学科学院卫生装备研究所 | Dynamic loading and circular perfusion bioreactor |
| CN202201907U (en) * | 2011-04-11 | 2012-04-25 | 中国人民解放军第二军医大学 | Cell stress culture apparatus |
| CN102759481A (en) * | 2012-06-26 | 2012-10-31 | 上海中医药大学附属岳阳中西医结合医院 | Multi-cell mechanical simulation experiment platform |
| CN102936567A (en) * | 2012-10-23 | 2013-02-20 | 重庆大学 | Microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation |
| CN104007029A (en) * | 2014-05-27 | 2014-08-27 | 华南理工大学 | Dynamic mechanical experimental device and method for tissue engineering scaffold |
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2018
- 2018-12-11 CN CN201811509283.7A patent/CN109706079B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201217661Y (en) * | 2008-03-28 | 2009-04-08 | 天津理工大学 | A bioreactor for dual-frequency loading of artificial cartilage |
| CN201817494U (en) * | 2010-08-10 | 2011-05-04 | 中国人民解放军军事医学科学院卫生装备研究所 | Dynamic loading and circular perfusion bioreactor |
| CN202201907U (en) * | 2011-04-11 | 2012-04-25 | 中国人民解放军第二军医大学 | Cell stress culture apparatus |
| CN102759481A (en) * | 2012-06-26 | 2012-10-31 | 上海中医药大学附属岳阳中西医结合医院 | Multi-cell mechanical simulation experiment platform |
| CN102936567A (en) * | 2012-10-23 | 2013-02-20 | 重庆大学 | Microtopological-structure plate flow chamber capable of applying electric and shearing force stimulation |
| CN104007029A (en) * | 2014-05-27 | 2014-08-27 | 华南理工大学 | Dynamic mechanical experimental device and method for tissue engineering scaffold |
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