CN109674835A - Angelica sinensis preparations, preparation method and applications with strengthen immunity - Google Patents
Angelica sinensis preparations, preparation method and applications with strengthen immunity Download PDFInfo
- Publication number
- CN109674835A CN109674835A CN201910170694.6A CN201910170694A CN109674835A CN 109674835 A CN109674835 A CN 109674835A CN 201910170694 A CN201910170694 A CN 201910170694A CN 109674835 A CN109674835 A CN 109674835A
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- CN
- China
- Prior art keywords
- radix angelicae
- angelicae sinensis
- water
- alcohol
- strengthen immunity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The present invention relates to a kind of angelica sinensis preparations with strengthen immunity, preparation method and applications.The angelica sinensis preparations with strengthen immunity include following component: Radix Angelicae Sinensis alcohol extract and Radix Angelicae Sinensis water extract.Above-mentioned angelica sinensis preparations have the function of strengthen immunity, also have the function of replenishing and activating blood, menstruction regulating and pain relieving.In addition, the stabilizing effect of the angelica sinensis preparations is good, not spirituosity, and it is at low cost, agriculture is residual, heavy metal is qualified, has no toxic side effect.
Description
Technical field
The present invention relates to Radix Angelicae Sinensis technical fields, more particularly to a kind of with the angelica sinensis preparations of strengthen immunity, preparation side
Method and its application.
Background technique
Radix Angelicae Sinensis is a kind of herbaceos perennial of Umbelliferae angelica.Its storage root dried is China's conventional Chinese medicine simply
Material, medicinal history is long, and history tree is on the books, and the note that begins calls its " Radix Angelicae Sinensis taste temperature, main hiccup on gas " in Shennong's Herbal
It is listed in middle product.It enriches blood and the effect of blood, regulating menstruation, hemostasis, ease constipation laxation, it is common for doctor, it is known as the title of " ten Fang Jiugui ".
Currently, Radix Angelicae Sinensis folk prescription product on the market be mainly Chinese angelica oral liquid based on Radix Angelicae Sinensis alcohol extracting liquid extract, Radix Angelicae Sinensis Tabellae,
Danggui Pills etc..The main function of such Radix Angelicae Sinensis folk prescription product be for female blood enriching promoting blood circulation, menstruction regulating and pain relieving, it is immune without having enhancing
The effect of power, application range are relatively narrow.
Summary of the invention
Based on this, it is necessary to aiming at the problem that Radix Angelicae Sinensis folk prescription products application narrow range, provide a kind of with strengthen immunity
Angelica sinensis preparations, preparation method and applications.
A kind of angelica sinensis preparations with strengthen immunity, including following component: Radix Angelicae Sinensis alcohol extract and Radix Angelicae Sinensis water extract.
Above-mentioned angelica sinensis preparations have the function of strengthen immunity, also have the function of replenishing and activating blood, menstruction regulating and pain relieving.In addition,
The stabilizing effect of the angelica sinensis preparations is good, not spirituosity, and at low cost, and agriculture is residual, heavy metal is qualified, has no toxic side effect.
The present invention also provides a kind of preparation methods of angelica sinensis preparations with strengthen immunity, include the following steps:
Alcohol or alcoholic solution are added into Radix Angelicae Sinensis, alcohol extract and the dregs of a decoction are respectively obtained after alcohol extracting, later by the alcohol extracting
Liquid concentration, drying, obtain Radix Angelicae Sinensis alcohol extract；
Water is added into the dregs of a decoction, water raises Aqueous extracts, later by Aqueous extracts concentration, drying, obtains Radix Angelicae Sinensis
Water extract；And
The Radix Angelicae Sinensis alcohol extract and the Radix Angelicae Sinensis water extract are mixed, preparation to get.
Angelica sinensis preparations are made after alcohol extracting and water mention in the preparation method of the above-mentioned angelica sinensis preparations with strengthen immunity, tool
There is the function of strengthen immunity, also has the function of replenishing and activating blood, menstruction regulating and pain relieving.
The addition alcohol into Radix Angelicae Sinensis or alcoholic solution further include before following steps in one of the embodiments: will
Radix Angelicae Sinensis crushes, is sieved, and obtains Radix Angelicae Sinensis coarse powder.
The solid-liquid ratio of the Radix Angelicae Sinensis and the alcohol or alcoholic solution is 1:(7-9 in one of the embodiments)；And/or
The alcoholic solution is the ethanol water that concentration is 75vol%-85vol%.
The temperature of the alcohol extracting is 80 DEG C -90 DEG C in one of the embodiments,；And/or
The number of the alcohol extracting is 1 time -3 times.
The solid-liquid ratio of the dregs of a decoction and the water is 1:(9-10 in one of the embodiments).
The temperature that the water mentions in one of the embodiments, is 90 DEG C -110 DEG C；And/or
The number that the water mentions is 1 time -3 times.
The mode of the drying is vacuum drying in one of the embodiments,；And/or
The vacuum drying temperature is 60 DEG C -65 DEG C.
The preparation method of angelica sinensis preparations with strengthen immunity in one of the embodiments, includes the following steps:
Radix Angelicae Sinensis is crushed, 10 meshes is crossed, obtains Radix Angelicae Sinensis coarse powder；60g Radix Angelicae Sinensis coarse powder is weighed, 480ml concentration, which is added, is
The ethanol water of 80vol%, heating and refluxing extraction 3h at 85 DEG C, respectively obtains the first alcohol extract and dregs of a decoction precursor；To described
It is added the ethanol water that 480ml concentration is 80vol% in dregs of a decoction precursor, heating and refluxing extraction 2h at 85 DEG C respectively obtains the
Two alcohol extracts and the dregs of a decoction；It is 1.25 that relative density is concentrated into after first alcohol extract and second alcohol extract are mixed, it
It is dried in vacuo at 60 DEG C afterwards, obtains Radix Angelicae Sinensis alcohol extract；
540ml water is added into the dregs of a decoction, heating and refluxing extraction 2h at 100 DEG C respectively obtains the first Aqueous extracts and water
Mention the dregs of a decoction；Addition 540ml water in the dregs of a decoction is mentioned to the water, heating and refluxing extraction 1h at 100 DEG C obtains the second Aqueous extracts；By institute
Stating and being concentrated into relative density after the first Aqueous extracts and second Aqueous extracts mix is 1.25, is dried in vacuo at 60 DEG C later,
Obtain Radix Angelicae Sinensis water extract；And
The Radix Angelicae Sinensis alcohol extract, the Radix Angelicae Sinensis water extract and 30g maltodextrin are mixed, concentration is added later is
The ethanol water granulation of 90vol%, it is dry to get.
The present invention also provides a kind of drugs with strengthen immunity, include the Radix Angelicae Sinensis system with strengthen immunity
Agent.
The angelica sinensis preparations with strengthen immunity are contained in said medicine, it is made also to have the function of strengthen immunity.
Detailed description of the invention
Fig. 1 is the flow chart of the preparation method of the angelica sinensis preparations with strengthen immunity of an embodiment of the present invention.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, to specific embodiment party of the invention
Formula is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But this hair
Bright to be implemented with being much different from other way described herein, those skilled in the art can be without prejudice in the present invention
Similar improvement is done in the case where culvert, therefore the present invention is not limited by the specific embodiments disclosed below.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term " and or " used herein includes one or more phases
Any and all combinations of the listed item of pass.
A kind of angelica sinensis preparations with strengthen immunity of an embodiment of the present invention, comprising: Radix Angelicae Sinensis alcohol extract and Radix Angelicae Sinensis
Water extract.
The chemical component of Radix Angelicae Sinensis mainly includes Ligustilide class and its isomeric compound, Coumarins, polysaccharide, flavonoids and has
Machine acids etc..Radix Angelicae Sinensis contain volatile oil component and organic acid, predominantly succinic acid, Yan acid, uracil, adenine, Ligustilide,
Vitamin B12, vitamin E, sitosterol, folinic acid, butylidenephthalide, ferulic acid/choline, 5- hy droxy furan aldehyde, microelement
The ingredients such as (such as zinc, copper, iron, manganese, potassium, sodium, calcium), phosphatide and vitamin A.The chemical component of angelica extract mainly includes
Ligustilide class and its isomeric compound, Coumarins, polysaccharide, flavonoids and organic acid etc..The effective component of angelica extract
For Ligustilide and normal-butyl.
In a wherein embodiment, angelica sinensis preparations further include auxiliary material.Auxiliary material can choose starch, maltodextrin etc..More
Preferably, auxiliary material is maltodextrin.
Preferably, the mass ratio of the quality of angelica extract (Radix Angelicae Sinensis alcohol extract+Radix Angelicae Sinensis water extract) and auxiliary material is 1:1.
Referring to Figure 1, the preparation method of a kind of angelica sinensis preparations with strengthen immunity of an embodiment of the present invention, packet
Include following steps:
S10, alcohol or alcoholic solution are added into Radix Angelicae Sinensis, alcohol extract and the dregs of a decoction are respectively obtained after alcohol extracting, later by alcohol extracting
Liquid concentration, drying, obtain Radix Angelicae Sinensis alcohol extract.
Wherein, further include following steps before alcohol or alcoholic solution are added into Radix Angelicae Sinensis: Radix Angelicae Sinensis being crushed, sieving, obtained
Radix Angelicae Sinensis coarse powder.
Preferably, 10 meshes are crossed after Radix Angelicae Sinensis smashes, and Radix Angelicae Sinensis coarse powder is made.In this way can preferably with alcohol or alcoholic solution
Mixing more evenly is conducive to the extraction of Radix Angelicae Sinensis alcohol extract.
Here alcoholic solution refers to the aqueous solution of alcohol.Specifically, the operation of alcohol extracting are as follows: heating and refluxing extraction collects alcohol
Extract.Herein, the obtained dregs of a decoction after alcohol extracting are to be centrifuged, and remove alcohol or alcoholic solution, are the next step Radix Angelicae Sinensis dregs of a decoction
Water mentions spare.
Preferably, the solid-liquid ratio of Radix Angelicae Sinensis and alcohol or alcoholic solution is 1:(7-9).
Preferably, alcoholic solution is the ethanol water that concentration is 75vol%-85vol%.Preferably, the temperature of alcohol extracting is
80 DEG C -90 DEG C, the number of alcohol extracting is 1 time -3 times.It is more advantageous to the extraction of the effective component in Radix Angelicae Sinensis in this way, and recovery rate is most
It is excellent.
Preferably, dry mode is vacuum drying, and vacuum drying temperature is 60 DEG C -65 DEG C.
S20, water is added into the dregs of a decoction, water raises Aqueous extracts, later by Aqueous extracts concentration, drying, obtains Radix Angelicae Sinensis water and mentions
Object.
Specifically, the operation that water mentions are as follows: heating and refluxing extraction collects Aqueous extracts.
Preferably, the solid-liquid ratio of the dregs of a decoction and water is 1:(9-10).The temperature that water mentions is 90 DEG C -110 DEG C.The number that water mentions is
1 time -3 times.Dry mode is vacuum drying, and dry temperature is 60 DEG C -65 DEG C.
S30, Radix Angelicae Sinensis alcohol extract and Radix Angelicae Sinensis water extract are mixed, preparation to get.
In general, when production angelica sinensis preparations also needing that auxiliary material is added.Preferably, angelica extract (Radix Angelicae Sinensis alcohol extract+Radix Angelicae Sinensis
Water extract) quality and auxiliary material mass ratio be 1:1.
Specifically, the operation of the step are as follows: by Radix Angelicae Sinensis alcohol extract, water extract and auxiliary materials and mixing, 90% ethanol water is added
Solution granulation, is placed in oven and dried later, obtains dry particle；Dry particle is put into capsule filling machine, is filled with
0.3g/ capsules, capsule are polished after filling, and are dispensed later using the medicinal polyester of oral administration solid, it is laggard
Row outer packing, inspection, storage.
Certainly, the type of preparation is not limited to capsule, can also be tablet, granule etc..
In a wherein embodiment, the preparation method of the angelica sinensis preparations with strengthen immunity includes the following steps:
Radix Angelicae Sinensis crushes, and crosses 10 meshes, obtains Radix Angelicae Sinensis coarse powder；60g Radix Angelicae Sinensis coarse powder is weighed, addition 480ml concentration is 80vol%
Ethanol water, heating and refluxing extraction 3h at 85 DEG C respectively obtains the first alcohol extract and dregs of a decoction precursor；Add into dregs of a decoction precursor
Enter the ethanol water that 480ml concentration is 80vol%, heating and refluxing extraction 2h at 85 DEG C respectively obtains the second alcohol extract and medicine
Slag；It is 1.25 that relative density is concentrated into after first alcohol extract and second alcohol extract are mixed, and vacuum is dry at 60 DEG C later
It is dry, obtain Radix Angelicae Sinensis alcohol extract.
540ml water is added into the dregs of a decoction, heating and refluxing extraction 2h at 100 DEG C respectively obtains the first Aqueous extracts and water mentions medicine
Slag；540ml water is added in the Xiang Shuiti dregs of a decoction, heating and refluxing extraction 1h at 100 DEG C obtains the second Aqueous extracts；By the first Aqueous extracts
It is 1.25 that relative density is concentrated into after mixing with the second Aqueous extracts, is dried in vacuo at 60 DEG C later, obtains Radix Angelicae Sinensis water extract.
Radix Angelicae Sinensis alcohol extract, water extract and 30g maltodextrin are mixed, it is water-soluble that the ethyl alcohol that concentration is 90vol% is added later
Liquid granulation, it is dry to get.
In this embodiment, the solid-liquid ratio for the ethanol water that Radix Angelicae Sinensis and concentration are 80vol% is 1:8, the dregs of a decoction and water
Solid-liquid ratio be 1:9, the quality of Radix Angelicae Sinensis alcohol extract and Radix Angelicae Sinensis water extract and be 1:1 with the mass ratio of maltodextrin.In addition, the reality
Applying the dregs of a decoction in mode is the ethanol water for being 80vol% by centrifugally operated removal concentration, and dregs of a decoction precursor and water mention medicine
Slag is by filter operation.
The preparation method of the above-mentioned angelica sinensis preparations with strengthen immunity, after progress alcohol extracting and water mention under given conditions
Angelica sinensis preparations are made, have the function of strengthen immunity, and have the function of replenishing and activating blood, menstruction regulating and pain relieving.
A kind of drug with strengthen immunity includes the angelica sinensis preparations with strengthen immunity.
The angelica sinensis preparations with strengthen immunity are contained in said medicine, it is made also to have the function of strengthen immunity.
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
Radix Angelicae Sinensis is crushed, 10 meshes is crossed, obtains Radix Angelicae Sinensis coarse powder；60g Radix Angelicae Sinensis coarse powder is weighed, 480ml concentration, which is added, is
The ethanol water of 80vol%, heating and refluxing extraction 3h at 85 DEG C, respectively obtains the first alcohol extract and dregs of a decoction precursor；To the dregs of a decoction
The ethanol water that 480ml concentration is 80vol% is added in precursor, heating and refluxing extraction 2h at 85 DEG C respectively obtains the second alcohol
Extract and the dregs of a decoction；It is 1.25 that relative density is concentrated into after first alcohol extract and second alcohol extract are mixed, later at 60 DEG C
Lower vacuum drying, obtains Radix Angelicae Sinensis alcohol extract.
540ml water is added into the dregs of a decoction, heating and refluxing extraction 2h at 100 DEG C respectively obtains the first Aqueous extracts and water mentions medicine
Slag；540ml water is added in the Xiang Shuiti dregs of a decoction, heating and refluxing extraction 1h at 100 DEG C obtains the second Aqueous extracts；By the first Aqueous extracts
It is 1.25 that relative density is concentrated into after mixing with the second Aqueous extracts, is dried in vacuo at 60 DEG C later, obtains Radix Angelicae Sinensis water extract.
Radix Angelicae Sinensis alcohol extract, Radix Angelicae Sinensis water extract and 30g maltodextrin are mixed, the ethyl alcohol that concentration is 90vol% is added later
Aqueous solution granulation, is placed in oven and dried later, obtains dry particle, dry particle is put into capsule filling machine, be filled with
0.3g/ capsules are to get angelica capsules.
Embodiment 2
Radix Angelicae Sinensis is crushed, 10 meshes is crossed, obtains Radix Angelicae Sinensis coarse powder；60g Radix Angelicae Sinensis coarse powder is weighed, 480ml concentration, which is added, is
The ethanol water of 85vol%, heating and refluxing extraction 3h at 80 DEG C, respectively obtains the first alcohol extract and dregs of a decoction precursor；To the dregs of a decoction
The ethanol water that 480ml concentration is 85vol% is added in precursor, heating and refluxing extraction 2h at 85 DEG C respectively obtains the second alcohol
Extract and the dregs of a decoction；It is 1.25 that relative density is concentrated into after first alcohol extract and the second alcohol extract are mixed, true at 65 DEG C later
Sky is dry, obtains Radix Angelicae Sinensis alcohol extract.
600ml water is added into the dregs of a decoction, heating and refluxing extraction 2h at 110 DEG C respectively obtains the first Aqueous extracts and water mentions medicine
Slag；600ml water is added in the Xiang Shuiti dregs of a decoction, heating and refluxing extraction 1h at 110 DEG C obtains the second Aqueous extracts；By the first Aqueous extracts
It is 1.25 that relative density is concentrated into after mixing with the second Aqueous extracts, is dried in vacuo at 65 DEG C later, obtains Radix Angelicae Sinensis water extract.
Radix Angelicae Sinensis alcohol extract, Radix Angelicae Sinensis water extract and 30g maltodextrin are mixed, the ethyl alcohol that concentration is 90vol% is added later
Aqueous solution granulation, is placed in oven and dried later, obtains dry particle, dry particle is put into capsule filling machine, be filled with
0.3g/ capsules are to get angelica capsules.
Embodiment 3
Radix Angelicae Sinensis is crushed, 10 meshes is crossed, obtains Radix Angelicae Sinensis coarse powder；60g Radix Angelicae Sinensis coarse powder is weighed, 420ml concentration, which is added, is
The ethanol water of 80vol%, heating and refluxing extraction 3h at 80 DEG C, respectively obtains the first alcohol extract and dregs of a decoction precursor；To the dregs of a decoction
The ethanol water that 420ml concentration is 80vol% is added in precursor, heating and refluxing extraction 2h at 80 DEG C respectively obtains the second alcohol
Extract and the dregs of a decoction；It is 1.25 that relative density is concentrated into after first alcohol extract and the second alcohol extract are mixed, true at 60 DEG C later
Sky is dry, obtains Radix Angelicae Sinensis alcohol extract.
540ml water is added into the dregs of a decoction, heating and refluxing extraction 2h at 90 DEG C respectively obtains the first Aqueous extracts and water mentions medicine
Slag；540ml water is added in the Xiang Shuiti dregs of a decoction, heating and refluxing extraction 1h at 90 DEG C obtains the second Aqueous extracts；By the first Aqueous extracts and
Second Aqueous extracts are concentrated into relative density after mixing be 1.25, is dried in vacuo at 60 DEG C later, obtains Radix Angelicae Sinensis water extract.
Radix Angelicae Sinensis alcohol extract, Radix Angelicae Sinensis water extract and 30g maltodextrin are mixed, the ethyl alcohol that concentration is 90vol% is added later
Aqueous solution granulation, is placed in oven and dried later, obtains dry particle, dry particle is put into capsule filling machine, be filled with
0.3g/ capsules are to get angelica capsules.
Comparative example 1
The Radix Angelicae Sinensis water of this comparative example 1 mentions capsule, and step similar embodiment 1, difference is that extraction process is only aqueous and mentions, and is free of
Alcohol extracting.
Angelica capsules study on the stability of the invention.
Using accelerated test, 37 ± 2 DEG C of temperature, relative humidity RH75 ± 5%, avoid light direct projection under conditions of store
3 months.0,1,2,3 month sample of placement is investigated.The results are shown in Table 1:
The quality standard of 1 angelica capsules of table
Angelica capsules of the invention under acceleration conditions, are placed 0 month, 1 month, 2 it can be seen from the above results
The moon, 3 months, testing product index meets the quality standard requirement, thus illustrates that angelica capsules folk prescription product of the invention is stablized
Property is good.
The compliance test result of the strengthen immunity of angelica capsules of the invention.
The test method Selection experiment and method of reference " health food is examined and assessment technique specification (version in 2003) ", into
The animal experiment of row strengthen immunity effect.Selected experimental project includes:
(1) weight, internal organs/weight ratio measurement: thymus gland/weight ratio, spleen/weight ratio
(2) cellular immune function assay: mouse spleen lymphocyte transformation experiment
(3) humoral immune function measures: serum hemolysin experiment
(4) monocytes/macrophages functional examination: mouse carbonic clearance experiment
(5) NK cytoactive detection: determination of lactate dehydrogenase method
1 materials and methods
1.1 samples: the angelica capsules sample sets (hereinafter referred to as sample group) of embodiment 1, the Radix Angelicae Sinensis water of comparative example 1 propose capsule-like
Product group (extraction process is only aqueous to be mentioned, and alcohol extracting, hereinafter referred to as water group are free of).Human body recommended intake is 6g/60kg BW/d, is removed
Outside negative control group, separately sets the unlimited pole increasing of market samples and exempt from oral solution (hereinafter referred to as market samples) as positive controls.With
The stomach-filling liquid of concentration needed for normal saline.
1.2 animals and grouping: SPF grades Balb/c mouse 336, mouse is first randomly divided into 4 groups in 18g-22g by weight,
Every group of the inside is further divided into 7 subgroups, receives saline control group, low sample group, middle sample group, high sample group respectively and (is equivalent to human body
5 times, 10 times, 20 times of recommended dose), middle water group, high water group (10 times, 20 times that are equivalent to human body recommended dose) and unlimited
Pole market samples group.
Stomach-filling amount: in low high dose group weigh respectively 2g, 4g, 8g sample be dissolved in 20ml physiological saline be made concentration difference
For the stomach-filling liquid of 0.1g/ml, 0.2g/ml, 0.4g/ml.
Feed: full nutrition mixed feed, animal freely ingest drinking-water, in the SPF grade animal house feeding that day and night change is 12h
It supports.
1.3 methods: it is examined according to strengthen immunity function in " health food is examined and assessment technique specification (version in 2003) "
Proved recipe method carries out.Give tested material stomach-filling volume be 20ml/kgBW, 1 time/d, continuous 30d.
1.3.1 the mouse lymphocyte conversion test (MTT) of ConA induction
Groups of animals continuous gavage 30d, cervical dislocation puts to death animal, sterile to take spleen, is placed in and fills appropriate sterile Hank ' s
In the plate of liquid, gently spleen is ground, individual cells suspension is made, through 200 mesh net filtrations, washes 2 times with Hank ' s liquid, every time
It is centrifuged 10min (1000r/min), then cell is suspended in 1ml RPMI1640 complete culture solution, with Trypan Blue meter
Number viable count (should be 95% or more), adjustment cell concentration are 3 × 106/ml.It is divided to two holes to be added every a cell suspension
In 24 well culture plates, every hole 1ml a, hole adds 75 μ l ConA liquid (being equivalent to 7.5 μ g/ml), another hole as control, set 37 DEG C,
5%CO272h is cultivated in incubator.Culture terminates preceding 4h, and every hole gently sucks supernatant 0.7ml, and 0.7ml is added and is free of small ox blood
Clear RPMI1640 culture solution, while 50 hole μ l/ MTT (5mg/ml) is added, continue to cultivate 4h.After culture, every hole is added
1ml acid isopropyl alcohol, piping and druming mix, and are completely dissolved purple crystal.Lysate is moved into 2ml cuvette, 722 spectrophotometrics
OD value is measured in wavelength 570nm on meter.
1.3.2 serolysin test
Groups of animals continuous gavage 30d prepares sheep red blood cell (SRBC) (SRBC) suspension of 2% (v/v), every mouse intraperitoneal injection
0.2ml is immunized.Continue to extract eyeball and take blood in centrifuge tube, place 1h after stomach-filling gives tested material 5d, by solidification blood with
Tube wall removing, is precipitated serum sufficiently, and 2000r/min is centrifuged 10min, collects serum.With physiological saline by serum doubling dilution,
The serum of different dilutions is respectively placed in Microhemagglutination breadboard, every 100 μ l of hole adds 100 μ l's 0.5% (v/v)
SRBC suspension mixes, and is packed into wet square position and covers, and in 37 DEG C of incubation 3h, observes hemagglutination degree.
1.3.3 mouse carbonic clearance test
Groups of animals continuous gavage 30d is diluted 4 times of india ink by every 10g weight 0.1ml tail vein injection.Injection
Timing immediately after prepared Chinese ink, and 20 μ l of blood is taken from angular vein clump respectively in 2min, 10min, it is added into 2ml 0.1%Na2CO3
In solution, 722 spectrophotometers measure OD value at 600nm wavelength, with 0.1%Na2CO3Solution makees blank control.Mouse
It puts to death, takes liver and spleen, blot organ surface blood stains with filter paper, weigh.Phagocytic index is calculated according to the following formula.
1.3.4 NK cytoactive detection
Groups of animals continuous gavage 30d, cervical dislocation puts to death animal, sterile to take spleen, is placed in and fills appropriate sterile Hank ' s
In the plate of liquid, gently spleen is ground, individual cells suspension is made, through 200 mesh net filtrations, washes 2 times with Hank ' s liquid, every time
It is centrifuged 10min (1000r/min), after splitting erythrocyte, Hank ' s liquid is added, 1000r/min is centrifuged 10min.Then by cell
It is suspended in 1ml RPMI1640 complete culture solution, with Trypan Blue living cell counting number (should be 95% or more), adjustment be thin
Born of the same parents' concentration is 2 × 107/ml.Target cell (Yac-1) is subjected to secondary culture for 24 hours before experiment, with RPMI1640 complete culture solution
Adjusting cell concentration is 4 × 105A/ml.Take target cell (Yac-1) and each 100 μ l of effector cell's (splenocyte) (effect target is than 50:
1) it, is added in U-shaped 96 well culture plate；Target cell Spontaneous release hole adds target cell and each 100 μ l of culture solution, the release of target cell maximum
Hole adds target cell and 1%NP40 or each 100 μ l of 2.5%Triton；Above-mentioned items are all provided with three parallel holes, in 37 DEG C, 5%CO2
4h is cultivated in incubator, 96 well culture plates is then centrifuged 5min with 1500r/min, 100 μ l horizontalization bottom 96 of supernatant is drawn in every hole
In well culture plate, while LDH matrix liquid 100ul is added, reacts 10min, the HCL30 μ l of 1mol/l is added in every hole, in microplate reader
OD value (OD) is measured at 490nm.
1.4 data processings: for statistical analysis to data using spss20
2 results
2.1 thymus gland/weight ratio and spleen/weight ratio
Sample is shown in Table 2.1.1 and table 2.1.2 to thymus gland/weight of Balb/c mouse and the influence of spleen/weight respectively.
The result shows that being remarkably improved mouse spleen/weight under middle sample, high sample group (10 times, 20 times that are equivalent to human body recommended dose)
Ratio；Water group (middle water, Gao Shui) can significantly reduce mouse chest it is dirty/weight ratio.It can be seen that the angelica capsules of embodiment 1
Sample sets can obviously increase immune system index and spleen index.Although angelica capsules sample sets do not obviously increase thymus index,
But for water group (significant decrease), better effect.
Table 2.1.1 angelica extract to the influence of mouse spleen/weight ratio (n=10,)
Table 2.1.2 angelica extract to the influence of mouse thymus/weight ratio (n=10,)
The mouse lymphocyte conversion test (MTT) of 2.2 ConA induction
It the results are shown in Table 2.2.1, which shows: angelica capsules sample sets (basic, normal, high sample group) OD of embodiment 1
Difference is above control group (physiological saline group), but does not have conspicuousness.Difference has significantly market samples group compared with the control group
Meaning, it can be deduced that market samples have the function of enhancing cellular immune function, and the angelica capsules sample of embodiment 1 is to cell
It is immune to have certain effect, but do not have conspicuousness.
Mouse lymphocyte transformation experiment that table 2.2.1 angelica extract induces ConA (n=9,)
2.3 serolysin test
The results are shown in Table 2.3.1, should experiments have shown that: compared with the control group, low sample, middle sample group and market samples group serum antibody
Horizontal group difference has statistical significance, and mean value is both greater than control group, it can be deduced that low sample, middle sample group and market samples group
Mice serum antibody level can be improved, has the function of enhancing humoral immune function.And other groups of samples do not have enhancing
The effect of humoral immune function.It may determine that the angelica capsules sample of embodiment 1 can enhance humoral immune function.
Table 2.3.1 angelica extract to mice serum antibody level influence (n=7 or 8,)
2.4 mouse carbonic clearance test
The results are shown in Table 2.4.1, should the results showed that compared with the control group, low sample, middle sample, high sample group macrophage
Phagocytic index is above control group, and middle sample group can significantly improve mouse monokaryon-macrophage function, other groups do not have raising
Mouse monokaryon-macrophage function.
Table 2.4.1 angelica extract to the influence of mouse monokaryon-macrophage function (n=9,)
2.5 NK cytoactive detections
It the results are shown in Table 2.5.1, it should be the results showed that compared with the control group, low sample, middle sample, high sample group can improve small
Mouse NK cell activity, and middle and high sample group has conspicuousness, it can be determined that angelica capsules sample can enhance NK cell activity.And city
Field sample can significantly reduce NK cell activity.
The influence active on NK cells in mice of table 2.5.1 angelica extract (n=9,)
3 conclusions
In conclusion index and spleen index can be improved in angelica capsules sample of the invention, enhance humoral immune function, enhancing NK is thin
Cytoactive has certain effect to cellular immune function and monocytes/macrophages function tool.General bureau is supervised according to food and medicine to do
Immune function method of inspection result judgement in " health food is examined and assessment technique specification (version in 2003) " of public Room publication
Principle can determine that angelica capsules of the invention have the function of strengthen immunity function.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of angelica sinensis preparations with strengthen immunity, which is characterized in that including following component: Radix Angelicae Sinensis alcohol extract and Radix Angelicae Sinensis water
Extract.
2. a kind of preparation method of the angelica sinensis preparations with strengthen immunity, which comprises the steps of:
Alcohol or alcoholic solution are added into Radix Angelicae Sinensis, respectively obtains alcohol extract and the dregs of a decoction after alcohol extracting, it is later that the alcohol extract is dense
Contracting, drying, obtain Radix Angelicae Sinensis alcohol extract；
Water is added into the dregs of a decoction, water raises Aqueous extracts, later by Aqueous extracts concentration, drying, obtains Radix Angelicae Sinensis water and mentions
Object；And
The Radix Angelicae Sinensis alcohol extract and the Radix Angelicae Sinensis water extract are mixed, preparation to get.
3. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that it is described to
Further include following steps before alcohol or alcoholic solution are added in Radix Angelicae Sinensis: Radix Angelicae Sinensis being crushed, sieving, obtain Radix Angelicae Sinensis coarse powder.
4. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that described to work as
Returning with the solid-liquid ratio of the alcohol or alcoholic solution is 1:(7-9)；And/or
The alcoholic solution is the ethanol water that concentration is 75vol%-85vol%.
5. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that the alcohol
The temperature mentioned is 80 DEG C -90 DEG C；And/or
The number of the alcohol extracting is 1 time -3 times.
6. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that the medicine
The solid-liquid ratio of slag and the water is 1:(9-10).
7. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that the water
The temperature mentioned is 90 DEG C -110 DEG C；And/or
The number that the water mentions is 1 time -3 times.
8. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that described dry
Dry mode is vacuum drying；And/or
The vacuum drying temperature is 60 DEG C -65 DEG C.
9. the preparation method of the angelica sinensis preparations according to claim 2 with strengthen immunity, which is characterized in that including such as
Lower step:
Radix Angelicae Sinensis is crushed, 10 meshes is crossed, obtains Radix Angelicae Sinensis coarse powder；60g Radix Angelicae Sinensis coarse powder is weighed, it is 80vol%'s that 480ml concentration, which is added,
Ethanol water, heating and refluxing extraction 3h at 85 DEG C, respectively obtains the first alcohol extract and dregs of a decoction precursor；Into the dregs of a decoction precursor
Be added the ethanol water that 480ml concentration is 80vol%, heating and refluxing extraction 2h at 85 DEG C, respectively obtain the second alcohol extract and
The dregs of a decoction；It is 1.25 that relative density is concentrated into after first alcohol extract and second alcohol extract are mixed, later at 60 DEG C
Vacuum drying, obtains Radix Angelicae Sinensis alcohol extract；
540ml water is added into the dregs of a decoction, heating and refluxing extraction 2h at 100 DEG C respectively obtains the first Aqueous extracts and water mentions medicine
Slag；Addition 540ml water in the dregs of a decoction is mentioned to the water, heating and refluxing extraction 1h at 100 DEG C obtains the second Aqueous extracts；By described
One Aqueous extracts and second Aqueous extracts are concentrated into relative density after mixing be 1.25, is dried in vacuo, obtains at 60 DEG C later
Radix Angelicae Sinensis water extract；And
The Radix Angelicae Sinensis alcohol extract, the Radix Angelicae Sinensis water extract and 30g maltodextrin are mixed, it is 90vol%'s that concentration is added later
Ethanol water granulation, it is dry to get.
10. a kind of drug with strengthen immunity, which is characterized in that there is strengthen immunity comprising described in claim 1
Angelica sinensis preparations.
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Citations (4)
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|---|---|---|---|---|
| EP0201784A2 (en) * | 1985-05-09 | 1986-11-20 | Yaguang Liu | Pharmaceutical composition decreasing side effects of anticancer chemotherapy and increasing the immune function |
| CN1376504A (en) * | 2002-04-10 | 2002-10-30 | 武汉大学 | Intravenous injection of Chinese angelica root and its preparing process |
| CN1537547A (en) * | 2003-04-14 | 2004-10-20 | 吕圭源 | Applicaton of chinese angelica-root for preparing medicine for treating hypertension disease |
| CN1876001A (en) * | 2005-06-07 | 2006-12-13 | 苏少宁 | Fresh angelica preparation and preparation method thereof |
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2019
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|---|---|---|---|---|
| EP0201784A2 (en) * | 1985-05-09 | 1986-11-20 | Yaguang Liu | Pharmaceutical composition decreasing side effects of anticancer chemotherapy and increasing the immune function |
| CN1376504A (en) * | 2002-04-10 | 2002-10-30 | 武汉大学 | Intravenous injection of Chinese angelica root and its preparing process |
| CN1537547A (en) * | 2003-04-14 | 2004-10-20 | 吕圭源 | Applicaton of chinese angelica-root for preparing medicine for treating hypertension disease |
| CN1876001A (en) * | 2005-06-07 | 2006-12-13 | 苏少宁 | Fresh angelica preparation and preparation method thereof |
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| 邓红娟等: "当归水提液和醇提液体外清除自由基的研究", 《甘肃农业大学学报》 * |
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Application publication date: 20190426 |