CN109645982A - Experimental rat cardiomyocyte action potential acquisition device - Google Patents

Abstract

The invention belongs to zoopery instrument fields, in particular to experimental rat Single Cardiac Cell acquisition device.Acquisition device of the invention includes puncture needle and butt-joint fixer, puncture needle and butt-joint fixer grafting；Acquisition method is used cooperatively by puncture needle and butt-joint fixer, produces the action potential signal of ventricular muscles.The present invention solves existing routine rat Single Cardiac Cell and needs to open thoracic cavity acquisition, it is high to operation technique and time requirement, time, slightly long then rat heart stopped bounce, the problems such as action potential of cardiac muscle cannot be collected, rat thoracic cavity need not be opened by developing one kind, can acquire the acquisition device and method of the action potential of cardiac muscle.

Description

Experimental rat cardiomyocyte action potential acquisition device

technical field

The invention belongs to the field of animal experiment equipment, and particularly relates to an action potential collection device of experimental rat cardiomyocytes.

Background technique

Animal experiments provide a theoretical basis for the progress of medical science. The action potential of cardiomyocytes is the basis for studying various pathogenic factors that cause changes in ECG. Conventional rat cardiomyocyte action potentials are collected by opening the thoracic cavity. The operation process requires rapidity and skill. If the time is slightly longer, the heart stops beating, and the action potentials of the myocardium cannot be collected.

SUMMARY OF THE INVENTION

Purpose of invention:

The present invention aims to provide an experimental rat cardiomyocyte action potential collection device, which can collect the action potential of cardiomyocytes without opening the rat's thoracic cavity.

Technical solutions:

The experimental rat myocardial cell action potential collection device comprises a puncture needle and a docking fixture, and the puncture needle is inserted into the docking fixture.

The lower end of the puncture needle made of steel as a whole is provided with a needle tip, an inverted water drop-shaped insulator is fixed above the needle tip, a needle handle is arranged on the puncture needle;

The needle length of the puncture needle is 30~40 mm, the length of the needle tip is 0.3~0.5 mm, the short diameter of the inverted water drop-shaped insulating material above the needle tip is 0.5~1.0 mm, the long diameter is 2.0~3.0 mm, and the length of the water droplet in the vertical direction is 2.5~4.0 mm; the needle handle The horizontal plane is oval, the coronal plane is trapezoid, and the sagittal plane is trapezoid.

The long axis of the ellipse-shaped needle handle is 8-12 mm on the long axis and 5-7 mm on the short axis; the long axis on the lower elliptical surface is 4-6 mm and the short axis is 5-7 mm; 20 mm; there is a rectangular plane on the elliptical cylinder of the needle handle, the length of the rectangular plane is 8-10 mm, and the width is 3-2 mm; the rectangular plane has a straight line parallel to the whole of the puncture needle.

There is a straight line parallel to the puncture needle on the rectangular plane of the needle handle. The straight line is parallel to the auxiliary straight line on the docking fixture, which can indicate the orientation of the puncture needle, so that the needle tip can be inserted into the needle hole at the distal end of the docking fixture to fix the puncture part. The middle rear is parallel to the proximal stem of the docking fixture.

The distal fixed puncture part of the "L"-shaped steel docking fixture is at an angle of 110 to 120 degrees with the proximal rod part. The distal fixed puncture part is provided with a needle hole in the shape of an inverted water droplet. The needle hole and the puncture needle are close to the needle tip. Inverted teardrop shaped insulation fits.

The thickness of the docking fixture is 0.1~0.4 mm; the length of the proximal rod is 30~50 mm, the thickness is 0.5~1 mm, and the width is 2~4 mm; blade.

The inner side of the pinhole is coated with an insulator substance; around the pinhole, there are a plurality of shallow-to-deep directing grooves from the outside to the inside.

An auxiliary straight line parallel to the straight line on the needle handle is arranged on the proximal rod portion of the butt-fixer.

A method for collecting action potentials of experimental rat cardiomyocytes, the collecting method specifically includes:

Step 1: The puncture needle is inserted into the thoracic cavity of the rat along the puncture point, and the docking fixture is inserted into the thoracic cavity from the next intercostal space;

Step 2: The straight line on the rectangular plane of the needle handle of the puncture needle and the auxiliary straight line on the docking fixture are on the same plane;

Step 3: The needle tip of the puncture needle is in contact with the distal fixed puncture part of the docking fixture, slides into the needle hole along the upper directional groove, and moves to the heart of the experimental rat together. There will be a sense of fluctuation; the upper wire of the puncture needle is connected to the ECG signal collector, and the action potential signal of the myocardial cell can be collected.

Advantages and Effects:

The present invention has the following advantages and beneficial effects:

The action potential of cardiomyocytes is the basis for studying various pathogenic factors that cause changes in electrocardiogram. The collection device of the present invention collects the action potentials of cardiomyocytes without opening the rat thoracic cavity. Rats can survive for a long time, and the puncture needle can be quickly positioned in the thoracic cavity of the rat with the docking fixture.

At the same time, the puncture needle is used in conjunction with the docking fixture to easily collect the action potential of ventricular cells, which can save a lot of experimental animals.

The collection device of the present invention does not need to open the rat's chest cavity, the collection process is rapid, and the collection position is accurate.

Description of drawings:

Figure 1 is a schematic diagram of the structure of the puncture needle.

Figure 2 is a schematic diagram of the structure of the docking fixture.

FIG. 3 is a schematic diagram of the actual use of the collection device of the present invention.

Figure 4 is a schematic diagram of the structure of the needle handle of the puncture needle.

FIG. 5 is a schematic diagram of the insertion of the pinhole and the inverted drop-shaped insulator.

Description of reference numbers:

1. Puncture needle; 2. Docking fixture; 3. Needle tip; 4. Inverted teardrop-shaped insulation; 5. Needle handle; 6. Guide wire; Hole; 10. Point to the groove; 11. Auxiliary straight line.

Detailed ways:

The present invention will be further described below in conjunction with the accompanying drawings:

As shown in Figure 1, Figure 2 and Figure 3, the experimental rat myocardial cell action potential collection device, the collection device includes a puncture needle 1 and a docking fixture 2, the puncture needle 1 and the docking fixture 2 are plugged; the plug is a The needle tip 3 of the finger puncture needle 1 is inserted into the rat chest cavity through the needle hole 9 of the docking fixture 2, and the water drop-shaped needle hole 9 and the inverted water drop-shaped insulating material 4 matching its shape are used to limit the depth of the needle tip 3 into the myocardium, preventing the needle tip from entering the myocardium. 3 Excessive penetration into the wall chamber.

The collection device of the present invention collects the action potentials of the rat cardiomyocytes through the cooperation of the puncture needle 1 and the docking fixture 2; the action potentials of the cardiomyocytes can be collected without opening the rat chest cavity.

As shown in FIG. 1 , the lower end of the puncture needle 1 made of steel as a whole is provided with a needle tip 3, an inverted water drop-shaped insulator 4 is fixed above the needle tip 3, the puncture needle 1 is provided with a needle handle 5; the upper end of the puncture needle 1 is provided with a Wire 6 connected to the electrical signal collector. The bottom tip of the puncture needle 1 is the needle tip 3. The function of the needle tip 3 is to penetrate the rat epidermis and collect the action potential of the cardiomyocytes. The inverted water drop-shaped insulator 4 above the needle tip 3 is made of hard material, which is not easy to deform, so as to ensure the puncture needle. 1 is inserted into the docking fixture 2, and will not be inserted too deep. A through hole is reserved in the middle of the needle handle 5. The needle handle 5 is fixed on the needle body of the puncture needle 1 through the through hole, and the needle handle 5 is arranged on the puncture needle 1. The middle and rear ends of the puncture needle 1 are generally 3 to 5 mm away from the top end of the puncture needle 1. The function of the needle handle 5 is that the operator holds the needle handle 5, which is not easy to slip, and the operation and control are more convenient.

The needle tip 3 of the puncture needle 1 and the puncture needle 1 are an integral structure, the inverted water drop-shaped insulator 4 is fixed on the puncture needle 1, the needle handle 5 is fixed on the upper end of the puncture needle 1, and the inverted water drop-shaped insulator 4 and the needle handle 5 are between. There is an insulating layer on the outside of the needle to prevent the electrical signals from being mixed into other parts of the rat's body. It can be seen from FIG. 2 that there is still a certain distance from the connection point of the wire 6 at the uppermost end of the puncture needle 1. The part above the puncture needle 1 that is not coated with an insulating layer is connected to the wire 6. The connection method can be welding, cross connection or other connection methods. The lead 6 is connected with the ECG signal collector.

As shown in Figure 1, the overall needle length of the puncture needle 1 is 30-40 mm, the length of the needle tip 3 is 0.3-0.5 mm, and the inverted drop-shaped insulating material 4 above the needle tip 3 has a short diameter of 0.5-1.0 mm and a long diameter of 2.0-3.0 mm. The length of the direction is 2.5-4.0 mm; the inverted water drop-shaped insulating material 4 can facilitate the entry of the puncture needle 1 into the thoracic cavity, and at the same time prevent the needle tip 3 from over-penetrating the ventricular wall after connecting the puncture hole. The upper surface of the needle tip 3 of the puncture needle 1 is covered with an insulating material, and the needle tip 3 is not covered with an insulating material to prevent other parts of the puncture needle 1 from conducting electricity, affecting the lead 6 connected to the upper end of the puncturing needle 1, and causing the myocardial action potential collected by the ECG signal collector to be inconsistent. allow.

As shown in Figure 4, the needle handle 5 is oval in the horizontal plane, trapezoidal in the coronal plane, and trapezoidal in the sagittal plane. The needle handle 5 is an insulator, the core of the puncture needle 1 is a conductor, and the puncture needle 1 is sequentially provided with a drop-shaped insulator 4 from the needle tip 3, the needle body part of the puncture needle 1, and the needle handle 5, and then connected to the ECG signal acquisition through the wire 6 device.

As shown in Fig. 4, the long axis of the ellipse-shaped needle handle 5 is 8-12 mm and the short axis is 5-7 mm; the long axis of the lower elliptical surface is 4-6 mm and the short axis is 5-7 mm; the needle handle 5 The length of the vertical direction is 18-20 mm; the elliptical cylinder of the needle handle 5 has a rectangular plane, the length of the rectangular plane is 8-10 mm, and the width is 3-2 mm; the rectangular plane has a whole parallel to the puncture needle. straight line. The rectangular plane is a rectangular plane cut out on the elliptical cylinder surface of the needle handle 5 , mainly for setting a straight line parallel to the needle body of the puncture needle 1 .

There is a straight line parallel to the puncture needle 1 on the rectangular plane of the needle handle 5. The straight line can be directly engraved on the puncture needle 1 or a straight line drawn by colored pigments. Similarly, the docking fixture 2 The auxiliary straight line 11 above is also set in the same way. The straight line on the rectangular plane is parallel to the auxiliary straight line 11 on the docking fixture 2, which can indicate the orientation of the puncture needle 1, so that the needle tip 3 is inserted into the docking fixture 2 and the distal end of the puncture part is fixed. The pinhole 9 of 7 is parallel to the proximal stem 8 of the docking fixture 2.

The angle between the distal fixed puncture part 7 and the proximal rod part 8 of the "L" shaped steel docking fixture 2 is 110-120 degrees, and the distal fixed puncture part 7 is provided with a needle hole in the shape of an inverted water drop. 9. The needle hole 9 is matched with the inverted water drop-shaped insulating material 4 of the puncture needle 1 near the needle tip 3. As shown in FIG. 5 , the fit means that the tip of the water droplet at the front end of the inverted drop-shaped insulator 4 matches the size of the pinhole 9 , keeping the shape fit without inserting the tip of the needle too deeply, that is, the depth of the tip of the needle is adjusted. Limitation, to prevent the needle tip from piercing the heart too deeply, causing the death of the rat; the needle hole 9 with the tip of the water droplet is a hole through which the distal fixed puncture part 7 is set through, and the needle hole 9 is a distance from the outer end of the distal fixed puncture part 7 It is 1.2~2.0 mm.

The thickness of the docking fixture 2 is 0.1-0.4 mm; the proximal rod part 8 is 30-50 mm long and 0.5-1 mm thick, while the tip of the distal-fixed puncture part 7 will be thinner and 2-4 mm wide; the distal-end fixation The puncture part 7 is 4 to 5 mm long, and the outer edge of the distal fixed puncture part 7 has a blade; the outer third of the distal fixed puncture part 7 is provided with a needle hole 9 in the shape of an inverted water droplet. The size of the needle hole 9 is consistent with the inverted water drop-shaped insulating material 4 of the puncture needle 1, which is beneficial to the puncture needle 1 to swing in different directions. As shown in FIG. 2 , the outer side of the distal fixing puncture portion 7 of the docking fixture 2 is a blade portion, which is convenient for the docking fixture 2 to pierce the thoracic cavity at the next intercostal space, so the thickness of the distal fixing puncturing portion 7 is thinner.

The inner side of the pinhole 9 is coated with an insulating substance; around the pinhole 9, there are a plurality of circumferentially directed grooves 10 from outside to inside from shallow to deep. The inner side of the pinhole 9 and the range of 1-2 mm around the pinhole 9 are covered with a hard insulator material to prevent the electrocardiographic signal from the rat body surface from being mixed and disturbed. Around the pinhole 9, there are several pointing grooves 10 evenly arranged in the circumferential direction with the pinhole 9 as the center. The pointing grooves 10 are from deep to shallow outward from the pinhole 9, which is convenient for the operator to confirm the position of the pinhole 9. , without visual observation. As shown in FIG. 2 , the number of pointing grooves 10 can be 4, 6, 8, etc. The insulating material can be an existing inorganic insulating paint, or a material such as rubber or silica gel, which is coated on the inside of the pinhole 9 and around the pinhole 9 .

An auxiliary straight line 11 parallel to the straight line on the needle handle 5 is provided on the proximal rod portion 8 of the butt-fixer 2 . The auxiliary straight line 11 is parallel to the straight line on the needle handle 5 of the puncture needle 1 when collecting the action potential of the cardiac cells.

As shown in Figure 3, a method for collecting action potentials of experimental rat cardiomyocytes, the collecting method specifically includes:

Step 1: After the rat is anesthetized, the puncture needle 1 is inserted into the thoracic cavity of the rat along the puncture point, and the docking fixture 2 is inserted into the thoracic cavity from the next intercostal space;

Step 2: The straight line on the rectangular plane at the front end of the sagittal plane of the needle handle 5 of the puncture needle 1 is on the same plane as the auxiliary straight line 11 on the docking fixture 2;

Step 3: The needle tip 3 of the puncture needle 1 contacts the distal fixed puncture part 7 of the docking fixture 2, slides into the needle hole 9 along the upper pointing groove 10, moves to the heart of the experimental rat together, and the needle tip 3 penetrates into the ventricle The finger end of the puncture needle 1 is connected to the electrocardiographic signal collector, and the action potential signal of the ventricular myocardium can be collected.

The advantage of using this collection method is that it is not necessary to open the rat's chest for collection, which prevents the rat's heart from beating due to a long operation time, and thus cannot collect the action potentials of the cardiomyocytes.

Claims (10)

1. experimental rat Single Cardiac Cell acquisition device, it is characterised in that: acquisition device includes puncture needle (1) and docking Fixator (2), puncture needle (1) and butt-joint fixer (2) grafting.

2. experimental rat Single Cardiac Cell acquisition device according to claim 1, it is characterised in that: generally steel Puncture needle (1) lower end of material is equipped with needle point (3), and needle point (3) is fixedly arranged above the insulant of falling water-drop-shaped (4), on puncture needle (1) Equipped with needle handle (5)；Puncture needle (1) upper end is equipped with the conducting wire (6) connecting with Electrocardial signal acquisition device.

3. experimental rat Single Cardiac Cell acquisition device according to claim 2, it is characterised in that: puncture needle (1) needle is 30 ~ 40 millimeters long, and needle point (3) is 0.3 ~ 0.5 millimeter long, and the insulant of falling water-drop-shaped (4) minor axis 0.5 above needle point (3) ~ 1.0 millimeters, 2.0 ~ 3.0 millimeters of major diameter, 2.5 ~ 4.0 millimeters of the length of vertical direction；Needle handle (5) horizontal plane is oval, coronal Face is trapezoidal, and sagittal plane is trapezoidal.

4. experimental rat Single Cardiac Cell acquisition device according to claim 3, it is characterised in that: elliptical cylinder-shape Needle handle (5) on 8 ~ 12 millimeters of ellipsoid long axis, 5 ~ 7 millimeters of short axle；Lower 4 ~ 6 millimeters of ellipsoid long axis, 5 ~ 7 millimeters of short axle；Needle The length of the vertical direction of handle (5) is 18 ~ 20 millimeters；There are a rectangular planes, rectangular planes on the elliptic cylinder of needle handle (5) Length be 8 ~ 10 millimeters, width is 3 ~ 2 millimeters；Rectangular planes have one and the whole parallel straight line of puncture needle (1).

5. experimental rat Single Cardiac Cell acquisition device according to claim 4, it is characterised in that: in needle handle (5) there is straight line parallel with puncture needle (1) together in rectangular planes, the straight line and the auxiliary on butt-joint fixer (2) are straight Line (11) in parallel, can indicate the orientation of puncture needle (1), and needle point (3) is made to inject the fixed puncture portion in butt-joint fixer (2) distal end (7) parallel with proximal stem portion (8) of butt-joint fixer (2) after in pin hole (9).

6. experimental rat Single Cardiac Cell acquisition device according to claim 1, it is characterised in that: " L " fashioned iron The distal end of the butt-joint fixer (2) of material fixed puncture portion (7) and proximal stem portion (8) are in 110 ~ 120 degree of angles, and distal end fixation is worn Thorn portion (7), which is equipped with to pour, drips pointed pin hole (9), the insulant of falling water-drop-shaped of pin hole (9) and puncture needle (1) close needle point (3) (4) it coincide.

7. experimental rat Single Cardiac Cell acquisition device according to claim 6, it is characterised in that: docking is fixed Device (2) is with a thickness of 0.1 ~ 0.4 millimeter；Proximal stem portion (8) is 30 ~ 50 millimeters long, 0.5 ~ 1 millimeter thick, 2 ~ 4 millimeters wide；Distal end is fixed Puncture portion (7) is 4 ~ 5 millimeters long, and fixed puncture portion (7) outer ledge in distal end has blade.

8. experimental rat Single Cardiac Cell acquisition device according to claim 6, it is characterised in that: pin hole (9) Inside is coated with insulator substance；The direction bar ditch (10) from shallow to deep of a plurality of circumferential direction from outside to inside are equipped with around pin hole (9).

9. experimental rat Single Cardiac Cell acquisition device according to claim 6 or 7, it is characterised in that: docking The proximal stem portion (8) of fixator (2) is equipped with the auxiliary straight line (11) of one with the straight line parallel on needle handle (5).

10. a kind of experimental rat Single Cardiac Cell acquisition method, it is characterised in that: the acquisition method specifically includes:

Step 1: puncture needle (1) is pierced into rat thoracic cavity along point of puncture, and butt-joint fixer (2) lunges thoracic cavity by next intercostal；

Step 2: the auxiliary straight line on straight line and butt-joint fixer (2) in needle handle (5) rectangular planes of puncture needle (1) (11) in same plane；

Step 3: the needle point (3) of puncture needle (1) is contacted with the distal end of butt-joint fixer (2) fixed puncture portion (7), along above It is directed toward bar ditch (10) to slide into pin hole (9), shifts to experimental rat heart together, needle point (3) lunges ventricle wall, the finger tip meeting of grip There is fluctuation；Puncture needle (1) upper end lead (6) is connect with Electrocardial signal acquisition device, can produce the action potential of cardiac muscle cell Signal.