CN109536377A - A kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal - Google Patents
A kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal Download PDFInfo
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- 238000005286 illumination Methods 0.000 claims description 14
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 abstract description 24
- 239000008280 blood Substances 0.000 abstract description 24
- 238000002428 photodynamic therapy Methods 0.000 abstract description 21
- 238000011160 research Methods 0.000 abstract description 16
- 238000011282 treatment Methods 0.000 abstract description 14
- 230000002792 vascular Effects 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 5
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- 230000000694 effects Effects 0.000 abstract description 2
- 239000003504 photosensitizing agent Substances 0.000 description 7
- 238000002347 injection Methods 0.000 description 5
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- 238000002360 preparation method Methods 0.000 description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 206010005003 Bladder cancer Diseases 0.000 description 1
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- 208000035868 Vascular inflammations Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 239000003292 glue Substances 0.000 description 1
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- BTXNYTINYBABQR-UHFFFAOYSA-N hypericin Chemical compound C12=C(O)C=C(O)C(C(C=3C(O)=CC(C)=C4C=33)=O)=C2C3=C2C3=C4C(C)=CC(O)=C3C(=O)C3=C(O)C=C(O)C1=C32 BTXNYTINYBABQR-UHFFFAOYSA-N 0.000 description 1
- 229940005608 hypericin Drugs 0.000 description 1
- PHOKTTKFQUYZPI-UHFFFAOYSA-N hypericin Natural products Cc1cc(O)c2c3C(=O)C(=Cc4c(O)c5c(O)cc(O)c6c7C(=O)C(=Cc8c(C)c1c2c(c78)c(c34)c56)O)O PHOKTTKFQUYZPI-UHFFFAOYSA-N 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3681—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by irradiation
- A61M1/3683—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by irradiation using photoactive agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N5/0613—Apparatus adapted for a specific treatment
- A61N5/062—Photodynamic therapy, i.e. excitation of an agent
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N2005/065—Light sources therefor
- A61N2005/0651—Diodes
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Abstract
本发明公开了一种体外光动力学细胞动物两用实验平台，涉及医疗器械技术领域，解决了由于致病细胞在人体血管系统中流动，无法直接进行光动力治疗的问题，其技术方案要点是：包括光动力照射组件和体外循环组件；光动力照射组件包括水平板、电源、变压器、LED灯、光栅、培养皿和激光功率计；电源与变压器电连接，变压器与LED灯电连接；水平板上表面设有培养支架，培养支架顶端设有承载件；激光功率计设有光强度探头；体外循环组件包括动脉插管、动脉导管、静脉导管、静脉插管和置于培养皿内的薄层玻片；薄层玻片内设有体外循环管路，具有将光敏药物干预后的研究对象的血液导出体外进行PDT治疗，防止PDT治疗损伤血管壁及周边组织的效果。
The invention discloses an in vitro photodynamic cell-animal dual-use experimental platform, which relates to the technical field of medical devices and solves the problem that photodynamic therapy cannot be performed directly due to the flow of pathogenic cells in the human vascular system. : Including photodynamic irradiation components and extracorporeal circulation components; photodynamic irradiation components include horizontal plate, power supply, transformer, LED lamp, grating, petri dish and laser power meter; power supply and transformer are electrically connected, and transformer and LED lamp are electrically connected; horizontal plate The upper surface is provided with a culture support, and the top of the culture support is provided with a carrier; the laser power meter is provided with a light intensity probe; the extracorporeal circulation components include arterial cannula, arterial catheter, venous catheter, venous cannula and a thin layer placed in the culture dish Glass slide: There is an extracorporeal circulation pipeline in the thin-layer glass slide, which has the effect of exporting the blood of the research subject after photosensitive drug intervention for PDT treatment, and preventing PDT treatment from damaging the blood vessel wall and surrounding tissues.
Description
Technical field
The present invention relates to the field of medical instrument technology, more specifically, it relates to a kind of ex-vivo photodynamic multicellular animal
Dual-purpose experiment porch.
Background technique
Photodynamic therapy (Photodynamic therapy, PDT) is a kind of novel Cancer Treatment Regimens.It is related to light
The quick dose of photoactivation in specific wavelength, photosensitizer can interact with molecular oxygen, generate singlet oxygen isoreactivity oxygen (reactive
Oxygen species ROS), and lead to death of neoplastic cells.The fluorescence diagnosis of different photosensitizers and photodynamic therapy can divide
Not Yong Yu a variety of diseases diagnosis or treatment, such as tumour, vascular diseases and inflammation etc..
Currently, PDT is only used for the diseases such as treatment cutaneum carcinoma due to being limited by medicine-feeding part and light irradiation；Nowadays new
The emergence of light source, so that photodynamic therapy (Hyp-PDT) that hypericin mediates (or the light that other photosensitizers mediate is dynamic
Mechanotherapy) it is used widely in the treatment of the kinds cancers such as bladder cancer, hypophysoma, glioma.
It, can not but since haemocyte and leukaemia cell are flowed in the sealing blood vessels system of human body
Direct irradiation, photodynamic therapy are difficult to apply to hematological system；Therefore, a kind of ex-vivo photodynamic multicellular animal how is designed
Dual-purpose experiment porch is our problems in the urgent need to address at present.
Summary of the invention
The object of the present invention is to provide a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal, have and intervene Hyp
PDT treatment is carried out after the blood export of research object afterwards is external, prevents the effect of PDT treatment damage vascular wall and perienchyma
Fruit.
Above-mentioned technical purpose of the invention has the technical scheme that a kind of ex-vivo photodynamic cell
The dual-purpose experiment porch of animal, including light power illumination module and extracorporal circulatory system component；
The smooth power illumination module includes level board, power supply, transformer, LED light, grating, culture dish and laser power
Meter；The output end of the power supply is electrically connected with the input terminal of transformer, and the output end of transformer and the input terminal of LED light are electrically connected
It connects；The LED light and grating are fixedly connected with level board, and grating is located above LED light；The level board upper surface is equipped with training
Bracket is supported, culture cantilever tip is equipped with the load-bearing part for culture dish holding；The laser power meter is equipped with for detecting culture
The luminous intensity of the photo-irradiation intensity of ware is popped one's head in；
The extracorporal circulatory system component includes arterial cannulation, arterial duct, ductus venosus, venous cannula and is placed in culture dish
Thin layer slide；Extracorporeal circulation pipeline is equipped in the thin layer slide；Described arterial duct one end is connected to arterial cannulation, another
End is connected to the input port of extracorporeal circulation pipeline；Described ductus venosus one end is connected to the output port of extracorporeal circulation pipeline,
The other end is connected to venous cannula.
By using above-mentioned technical proposal, using transformer, stable direct current is provided for the LED light irradiation light of specific wavelength
Electricity, the stability of wavelength light needed for guaranteeing excitation photosensitizer；Using grating, convenient for adjusting the illumination of LED light irradiation culture dish
Intensity；Using load-bearing part, it is convenient for culture dish holding；It is popped one's head in using laser power meter and luminous intensity, is convenient for real-time detection culture dish
Photo-irradiation intensity；Using extracorporeal circulation pipeline, thin layer slide is placed in culture dish, blood is always moved from the neck of research object
It is then quiet from stock followed by arterial cannulation, arterial duct, extracorporeal circulation pipeline, ductus venosus and venous cannula after arteries and veins is drawn
Arteries and veins flows back in research object body；Convenient for the research object after intervening photosensitive drug blood export it is external after carry out PDT treatment,
The case where preventing PDT treatment damage vascular wall and perienchyma generation.
The present invention is further arranged to: the load-bearing part is transparent grid.
By using above-mentioned technical proposal, using transparent grid, reduces load-bearing part refraction or absorb the feelings of LED light irradiation light
Condition occurs, and improves the stability of LED light irradiation culture dish.
The present invention is further arranged to: the level board upper surface is sealedly connected with transparent cover, and culture dish is placed in transparent
In cover.
By using above-mentioned technical proposal, subtracted using transparent cover so that culture dish is in metastable research environment
The case where few extraneous factor influences result of study occurs.
The present invention is further arranged to: the level board is fixedly connected with the first fan below LED light；The water
Plate is fixedly connected with support base, and support base is fixedly connected with the second fan, and level board is equipped with thermovent.
By using above-mentioned technical proposal, using the first fan and the second fan, avoid because temperature increases in experiment porch
And the case where influencing result of study generation.
The present invention is further arranged to: the grating includes adjusting ring, bottom plate and multiple adjustable plates；The bottom plate and level
Plate is fixedly connected, and adjusts ring and bottom plate rotation connects；The adjusting ring inner wall is equipped with ring gear, and plate upper surface rotation connects
It is connected to rotation axis, rotation axis is coaxially arranged with the driven gear engaged with ring gear, and adjustable plate is fixedly connected with the rotating shaft；It is more
Along ring gear circumferencial direction, successively stacked offset is arranged a adjustable plate, and adjustable plate is arranged in a one-to-one correspondence with rotation axis.
It by using above-mentioned technical proposal, is engaged using ring gear with driven gear, convenient for driving multiple adjustings simultaneously
Plate rotation, convenient for adjusting the size of the illumination loophole between multiple adjustable plates, so that the illumination for adjusting LED light irradiation culture dish is strong
It spends easy to operate.
The present invention is further arranged to: the thin layer slide includes the first slotting body and the second slotting body being mutually inserted；First
It inserts body and the second slotting body symmetric side is fixedly connected with transparent glass, the first slotting body, the second slotting body and two transparent glass enclose
Form extracorporeal circulation pipeline.
By using above-mentioned technical proposal, convenient for the situation of change of pathogenic cell in blood in observation extracorporeal circulation pipeline.
The present invention is further arranged to: the extracorporeal circulation pipeline is broken line type pipeline.
By using above-mentioned technical proposal, convenient for extending the light irradiation time of blood in extracorporeal circulation pipeline.
The present invention is further arranged to: the extracorporeal circulation pipeline section is rectangular-shaped.
By using above-mentioned technical proposal, convenient for improving the irradiated area of blood in extracorporeal circulation pipeline, so that following in vitro
Blood in uniform light in endless tube road.
The present invention is further arranged to: the arterial duct is equipped with triple valve, and triple valve is provided with and communicated with to be connected for syringe
The injection pipeline connect.
By using above-mentioned technical proposal, using triple valve and injection pipeline, convenient for injecting assistant medicament into blood；Together
When so that blood preparation take it is easy to operate.
In conclusion the invention has the following advantages: providing stabilization using transformer for the LED light of specific wavelength
Direct current, guarantee excitation photosensitizer needed for wavelength light stability；Using grating, culture dish is irradiated convenient for adjusting LED light
Intensity of illumination；Using load-bearing part, it is convenient for culture dish holding；It is popped one's head in using laser power meter and luminous intensity, is convenient for real-time detection
The photo-irradiation intensity of culture dish；Using extracorporeal circulation pipeline, thin layer slide is placed in culture dish, blood is from research object
After arteria carotis communis is drawn, followed by arterial cannulation, arterial duct, extracorporeal circulation pipeline, ductus venosus and venous cannula, then
It is flowed back in research object body from femoral vein；Convenient for the research object after intervening Hyp blood export it is external after carry out PDT and control
The case where treating, preventing PDT treatment damage vascular wall and perienchyma generation；Using transparent grid, reduces load-bearing part refraction or inhale
The case where receiving LED light irradiation light occurs；It is engaged using ring gear with driven gear, convenient for driving multiple adjustable plates to turn simultaneously
It is dynamic, convenient for adjusting the size of the illumination loophole between multiple adjustable plates；Using triple valve and injection pipeline, convenient for being infused into blood
Penetrate assistant medicament；Meanwhile so that blood preparation take it is easy to operate.
Detailed description of the invention
Fig. 1 is the overall structure diagram in the embodiment of the present invention；
Fig. 2 is the structural schematic diagram of extracorporal circulatory system component in the embodiment of the present invention；
Fig. 3 is the overall structure diagram of grating in the embodiment of the present invention；
Fig. 4 is the partial schematic diagram after grating is splitted in the embodiment of the present invention.
In figure: 1, level board；11, transparent cover；12, support base；2, power supply；21, transformer；22, LED light；23, light
Grid；231, bottom plate；232, ring is adjusted；233, adjustable plate；234, swivelling chute；235, rotation axis；236, driven gear；237, annular
Gear；24, bracket is cultivated；25, load-bearing part；26, culture dish；27, lid is cultivated；28, laser power meter；29, luminous intensity is popped one's head in；
3, the first fan；31, the second fan；32, thermovent；4, extracorporal circulatory system component；41, the first slotting body；42, the second slotting body；43, thoroughly
Bright glass；44, extracorporeal circulation pipeline；45, input port；451, arterial duct；452, arterial cannulation；46, output port；461,
Ductus venosus；462, venous cannula, 47, triple valve；48, injection pipeline.
Specific embodiment
Below in conjunction with attached drawing 1-4, invention is further described in detail.
Embodiment: a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal, as shown in Figure 1, including that light power irradiates
Component and extracorporal circulatory system component 4.Light power illumination module include level board 1, power supply 2, transformer 21, LED light 22, grating 23,
Culture dish 26 and laser power meter 28.The output end of power supply 2 is electrically connected with the input terminal of transformer 21, the output end of transformer 21
It is electrically connected with the input terminal of LED light 22.LED light 22 and grating 23 are fixedly connected with level board 1, and grating 23 is located at LED light 22
Top.1 upper surface of level board is equipped with culture bracket 24, and culture 24 top of bracket is equipped with the load-bearing part for culture dish holding 26
25.Laser power meter 28 is equipped with the luminous intensity probe 29 of the photo-irradiation intensity for detecting culture dish 26.Culture dish 26 is covered with
Lid 27 is cultivated, can be used for individually studying blood cell.Using transformer 21, provided for 22 irradiation light of LED light of specific wavelength
Stable DC, the stability of wavelength light needed for guaranteeing excitation photosensitizer；Using grating 23, shone convenient for adjusting LED light 22
Penetrate the intensity of illumination of culture dish 26.Using load-bearing part 25, it is convenient for culture dish holding 26.It is visited using laser power meter 28 and luminous intensity
First 29, convenient for the photo-irradiation intensity of real-time detection culture dish 26.
As shown in Fig. 2, extracorporal circulatory system component 4 is inserted including arterial cannulation 452, arterial duct 451, ductus venosus 461, vein
Pipe 462 and the thin layer slide being placed in culture dish 26.Extracorporeal circulation pipeline 44 is equipped in thin layer slide.451 one end of arterial duct
It is connected to arterial cannulation 452, the other end is connected to the input port 45 of extracorporeal circulation pipeline 44.461 one end of ductus venosus and body
The output port 46 of external circulation line 44 is connected to, and the other end is connected to venous cannula 462.It, will be thin using extracorporeal circulation pipeline 44
Layer slide is placed in culture dish 26, and blood followed by arterial cannulation 452, moves after the extraction of the arteria carotis communis of research object
Arteries and veins conduit 451, extracorporeal circulation pipeline 44, ductus venosus 461 and venous cannula 462, then flow back to research object body from femoral vein
It is interior.Convenient for the research object after intervening Hyp blood export it is external after carry out PDT treatment, prevent PDT treatment damage vascular wall
And the case where perienchyma, occurs.
As shown in Figure 1, the load-bearing part 25 in the present embodiment uses transparent grid.Using transparent grid, load-bearing part 25 is reduced
The case where refraction or absorption 22 irradiation light of LED light, occurs, and improves the stability that LED light 22 irradiates culture dish 26.
As shown in Figure 1,1 upper surface of level board is sealedly connected with transparent cover 11, culture dish 26 is placed in transparent cover 11.
Culture bracket 24 in the present embodiment uses transparent glass infuser.Using transparent cover 11, so that culture dish 26 is in relatively steady
Fixed research environment reduces the case where extraneous factor influences result of study and occurs.
As shown in Figure 1, level board 1 is fixedly connected with the first fan 3 positioned at 22 lower section of LED light.Level board 1 is fixedly connected
There is support base 12, support base 12 is fixedly connected with the second fan 31, and level board 1 is equipped with thermovent 32.Utilize 3 He of the first fan
Second fan 31 avoids the case where influencing result of study due to temperature increases in experiment porch generation.
As shown in Figure 3 and Figure 4, grating 23 includes adjusting ring 232, bottom plate 231 and multiple adjustable plates 233.Bottom plate 231 and water
Plate 1 (referring to Fig.1) is fixedly connected, and 231 top surface edge of bottom plate is equipped with the swivelling chute 234 rotated for adjusting ring 232.It adjusts
232 inner wall of ring is equipped with ring gear 237, and 231 upper surface of bottom plate is rotatably connected to rotation axis 235, and rotation axis 235 is coaxially disposed
There is the driven gear 236 engaged with ring gear 237, adjustable plate 233 is fixedly connected with rotation axis 235.Multiple 233 edges of adjustable plate
Successively stacked offset is arranged 237 circumferencial direction of ring gear, and adjustable plate 233 is arranged in a one-to-one correspondence with rotation axis 235.Utilize annular
Gear 237 is engaged with driven gear 236, convenient for driving multiple adjustable plates 233 to rotate simultaneously, convenient for adjusting multiple adjustable plates 233
Between illumination loophole size so that adjust LED light 22 irradiate culture dish 26 intensity of illumination it is easy to operate.
As shown in Fig. 2, thin layer slide includes the first slotting body 41 and the second slotting body 42 being mutually inserted.First slotting body 41 and
Two slotting 42 symmetric sides of body are fixedly connected with transparent glass 43, and the first slotting body 41, the second slotting body 42 and two transparent glass 43 enclose
Conjunction forms extracorporeal circulation pipeline 44.Transparent glass 43 and the first slotting body 41 and the second slotting body 42 pass through nontoxic waterproof glue bond.This
The first slotting body 41 in embodiment inserts body using class " W " type, and the second slotting body 42 in the present embodiment inserts body using class U-typed.This
The first slotting body 41 and the second slotting body 42 in embodiment are made of PP material.Convenient in blood in observation extracorporeal circulation pipeline 44
The situation of change of pathogenic cell.
As shown in Fig. 2, the extracorporeal circulation pipeline 44 in the present embodiment uses broken line type pipeline.Convenient for extending extracorporeal circulation set tube
The light irradiation time of blood in road 44.
As shown in Fig. 2, the section of extracorporeal circulation pipeline 44 is rectangular-shaped in the present embodiment.The output of extracorporeal circulation pipeline 44
The width of port 46 and input port 45 is greater than the diameter of arterial duct 451 and ductus venosus 461.Extracorporeal circulation pipeline 44 it is defeated
The thickness of exit port 46 and input port 45 is less than the diameter of arterial duct 451 and ductus venosus 461.Convenient for improving extracorporal circulatory system
The irradiated area of blood in pipeline 44, so that the blood in uniform light in extracorporeal circulation pipeline 44.
As shown in Fig. 2, arterial duct 451 is equipped with triple valve 47, triple valve 47 is provided with and communicated with the note for syringe connection
Penetrate pipeline 48.Using triple valve 47 and injection pipeline 48, convenient for injecting assistant medicament into blood；Meanwhile so that blood preparation
It takes easy to operate.
Working principle: utilizing transformer 21, provide stable DC for 22 irradiation light of LED light of specific wavelength, guarantees
The stability of wavelength light needed for exciting photosensitizer；Using grating 23, the illumination for irradiating culture dish 26 convenient for adjusting LED light 22 is strong
Degree.Using load-bearing part 25, it is convenient for culture dish holding 26.Using laser power meter 28 and luminous intensity probe 29, it is convenient for real-time detection
The photo-irradiation intensity of culture dish 26.Using extracorporeal circulation pipeline 44, thin layer slide is placed in culture dish 26, blood is from research
After the arteria carotis communis of object is drawn, followed by arterial cannulation 452, arterial duct 451, extracorporeal circulation pipeline 44, ductus venosus
461 and venous cannula 462, then flowed back in research object body from femoral vein.Convenient for the blood of the research object after intervening Hyp
The case where carrying out PDT treatment after export is external, preventing PDT treatment damage vascular wall and perienchyma generation.
This specific embodiment is only explanation of the invention, is not limitation of the present invention, those skilled in the art
Member can according to need the modification that not creative contribution is made to the present embodiment after reading this specification, but as long as at this
All by the protection of Patent Law in the scope of the claims of invention.
Claims (9)
1. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal, it is characterized in that: including light power illumination module and in vitro
Recirculation assembly (4)；
The smooth power illumination module includes level board (1), power supply (2), transformer (21), LED light (22), grating (23), training
Support ware (26) and laser power meter (28)；The output end of the power supply (2) is electrically connected with the input terminal of transformer (21), transformer
(21) output end is electrically connected with the input terminal of LED light (22)；The LED light (22) and grating (23) are solid with level board (1)
Fixed connection, grating (23) are located above LED light (22)；Level board (1) upper surface is equipped with culture bracket (24), cultivates bracket
(24) top is equipped with the load-bearing part (25) for culture dish holding (26)；The laser power meter (28) is equipped with for detecting culture
The luminous intensity of the photo-irradiation intensity of ware (26) pops one's head in (29)；
The extracorporal circulatory system component (4) includes arterial cannulation (452), arterial duct (451), ductus venosus (461), venous cannula
(462) and the thin layer slide that is placed in culture dish (26)；Extracorporeal circulation pipeline (44) are equipped in the thin layer slide；The artery
Conduit (451) one end is connected to arterial cannulation (452), and the other end is connected to the input port (45) of extracorporeal circulation pipeline (44)；
Described ductus venosus (461) one end is connected to the output port (46) of extracorporeal circulation pipeline (44), the other end and venous cannula
(462) it is connected to.
2. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal according to claim 1, it is characterized in that: described hold
Holder (25) is transparent grid.
3. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal according to claim 1, it is characterized in that: the water
Plate (1) upper surface is sealedly connected with transparent cover (11), and culture dish (26) is placed in transparent cover (11).
4. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal according to claim 3, it is characterized in that: the water
Plate (1) is fixedly connected with the first fan (3) below LED light (22)；The level board (1) is fixedly connected with support base
(12), support base (12) is fixedly connected with the second fan (31), and level board (1) is equipped with thermovent (32).
5. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal according to claim 1, it is characterized in that: the light
Grid (23) include adjusting ring (232), bottom plate (231) and multiple adjustable plates (233)；The bottom plate (231) and level board (1) are fixed
Connection adjusts ring (232) and bottom plate (231) rotation and connects；Adjusting ring (232) inner wall is equipped with ring gear (237), bottom
Plate (231) upper surface is rotatably connected to rotation axis (235), and rotation axis (235), which is coaxially arranged with, to be engaged with ring gear (237)
Driven gear (236), adjustable plate (233) are fixedly connected with rotation axis (235)；Multiple adjustable plates (233) are along ring gear
(237) successively stacked offset is arranged circumferencial direction, and adjustable plate (233) is arranged in a one-to-one correspondence with rotation axis (235).
6. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal described in -5 any one according to claim 1, special
Sign is: the thin layer slide includes the first slotting body (41) and the second slotting body (42) being mutually inserted；First slotting body (41) and second
It inserts body (42) symmetric side to be fixedly connected with transparent glass (43), the first slotting body (41), the second slotting body (42) and two transparent glass
Glass (43) is enclosed extracorporeal circulation pipeline (44).
7. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal described in -5 any one according to claim 1, special
Sign is: the extracorporeal circulation pipeline (44) is broken line type pipeline.
8. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal described in -5 any one according to claim 1, special
Sign is: extracorporeal circulation pipeline (44) section is rectangular-shaped.
9. a kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal described in -5 any one according to claim 1, special
Sign is: the arterial duct (451) is equipped with triple valve (47), and triple valve (47) is provided with and communicated with the injection-tube for syringe connection
Road (48).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811249625.6A CN109536377A (en) | 2018-10-25 | 2018-10-25 | A kind of dual-purpose experiment porch of ex-vivo photodynamic multicellular animal |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8654429B2 (en) * | 2010-12-21 | 2014-02-18 | Canon Denshi Kabushiki Kaisha | Light amount adjustment apparatus and optical apparatus |
| CN106880881A (en) * | 2017-03-06 | 2017-06-23 | 赵磊 | A kind of extracorporeal blood photodynamic therapy device |
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2018
- 2018-10-25 CN CN201811249625.6A patent/CN109536377A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8654429B2 (en) * | 2010-12-21 | 2014-02-18 | Canon Denshi Kabushiki Kaisha | Light amount adjustment apparatus and optical apparatus |
| CN106880881A (en) * | 2017-03-06 | 2017-06-23 | 赵磊 | A kind of extracorporeal blood photodynamic therapy device |
Non-Patent Citations (2)
| Title |
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| YIXIAO XU等: "Hypericin-mediated photodynamic therapy induces apoptosis in K562 human leukemia cells through JNK pathway modulation", 《MOLECULAR MEDICINE REPORTS》 * |
| 许益笑: "经体外环路光动力疗法对白血病细胞的杀伤作用及机制研究", 《中国博士学位论文全文数据库医药卫生科技辑》 * |
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Application publication date: 20190329 |