CN109536374A

CN109536374A - It is a kind of with avoid bubble generate structure reagent reaction tube

Info

Publication number: CN109536374A
Application number: CN201811424503.6A
Authority: CN
Inventors: 王炜; 尚保华; 梁保涛; 王长龙; 汪练
Original assignee: Nanjing Institute of Advanced Laser Technology
Current assignee: Nanjing Institute of Advanced Laser Technology
Priority date: 2018-11-27
Filing date: 2018-11-27
Publication date: 2019-03-29

Abstract

The invention discloses a reagent reaction tube with a structure to avoid bubble generation, comprising a liquid inlet port, a liquid inlet conduit, a reaction chamber, an air outlet conduit and an air outlet port; the liquid inlet conduit is connected to the liquid inlet port to the reaction chamber, and the gas outlet port The conduit connects the reaction chamber to the gas outlet port. The invention provides a device for discharging bubbles along the airway based on the surface tension of the bubble liquid and the thrust of the liquid flow. The contradiction between the easy generation of air bubbles in the channel and the narrow channel.

Description

It is a kind of with avoid bubble generate structure reagent reaction tube

Technical field

The present invention relates to a kind of reagent reaction tube more particularly to it is a kind of with avoid bubble generate structure reagent reaction Pipe.

Background technique

The research of nucleic acid purification and real-time fluorescence PCR reaction integrated instrument is applied due to its high degree of automation, reaction Process is pollution-free, and oneself is increasingly becomed the main direction of development of real-time fluorescence PCR instrument, but by the sample of nucleic acid of purification from turn It is easy to leave air generation bubble in reaction tube when moving on in PCR reagent pipe, directly affects experimental result, how not increase Avoided in the case where experimental arrangement bubble generation be PCR reaction tube structure design a great problem, constrain this technology Using and promote.

Summary of the invention

Goal of the invention: in view of the above problems, the present invention propose it is a kind of with avoid bubble generate structure reagent reaction tube, Overcome the lance that bubble is also easy to produce in the slype and slype that design to reduce the loss of reaction reagent in pipetting processes Shield.

Technical solution: to achieve the purpose of the present invention, the technical scheme adopted by the invention is that: one kind, which has, avoids bubble Generate the reagent reaction tube of structure, including feed liquor interface, liquid inlet conduit, reaction chamber, outtake tube and outlet interface；The feed liquor Conduit connects feed liquor interface to reaction chamber, and the outtake tube connects reaction chamber to outlet interface.

Further, the feed liquor interface and outlet interface are the cylinder structure of prominent reagent reaction tube.

Further, the middle part of the feed liquor interface and outlet interface is equipped with trip.

Further, the liquid inlet conduit and outtake tube are in U-shape distribution in reagent reaction tube.

Further, the lower left corner of the reaction chamber is communicated with liquid inlet conduit, the upper right corner and the outtake tube phase of reaction chamber Logical, the upper left corner and the lower right corner of reaction chamber are fillet design.

Further, the reagent reaction tube side wall is sealed with PVC film.

Make bubble along air guide based on bubble liquid surface tension and liquid flow dynamicthrust the utility model has the advantages that the present invention provides one kind The device of pipe direction discharge, can be used for the research and development of real-time fluorescence PCR detection instrument, overcome to reduce and reacting examination in pipetting processes The loss of agent and the contradiction that bubble is also easy to produce in the slype and slype that design.

Detailed description of the invention

Fig. 1 is structural schematic diagram of the invention；

Fig. 2 is that feed liquor reagent of the invention flows to schematic diagram.

Specific embodiment

Further description of the technical solution of the present invention with reference to the accompanying drawings and examples.

As shown in Figure 1, a kind of have the reagent reaction tube for avoiding bubble from generating structure applied to real-time fluorescence PCR instrument, Including feed liquor interface 1, liquid inlet conduit 2, reaction chamber 3, outtake tube 4 and outlet interface 5.Reagent reaction tube side wall PVC film into Row sealing.

The positional relationship of above-mentioned each element is as follows: feed liquor interface 1, outlet interface 5 are that the cylinder structure of prominent reaction tube is used It is connect in reagent reaction tube with sample introduction mechanism, liquid inlet conduit 2 connects feed liquor interface 1 to reaction chamber 3, the connection reaction of outtake tube 4 Outlet interface 5 is arrived in room 3.The middle part of feed liquor interface 1 and outlet interface 5 is equipped with trip, can be with fixating reagent reaction tube.Liquid inlet conduit 2 and outtake tube 4 be in U-shape in reagent reaction tube distribution.

The lower left corner of reaction chamber 3 is communicated with liquid inlet conduit 2, and the upper right corner of reaction chamber 3 is communicated with outtake tube 4, reaction chamber The upper left corner and the lower right corner be fillet design, can be to avoid the generation of bubble.

When the sample of nucleic acid of purification is pushed into reaction tube from feed liquor interface 1, since surface tension liquid is full of narrow feed liquor Air extrusion in channel to PCR reaction chamber 3 after liquid enters PCR reaction chamber 3, is compared due to reaction chamber and is led by conduit 2 Tube passage wants wide very much, and liquid collects in reaction chamber bottom after entering reaction chamber under surface tension effects, full of slow behind bottom The air extrusion at top is entered outtake tube 4 by slow rise, and until liquid is full of PCR reaction chamber 3, liquid just enters outtake tube 4, The closed of reagent in PCR reaction chamber 3 is realized by the U-shaped structure of outtake tube 4, even if the volume in PCR reaction process is swollen Swollen contraction does not have gas and enters in PCR reaction chamber 3.

Using deionized water, the deionized water of bubble-free is injected by the feed liquor interface 1 in reaction tube, by PCR reaction chamber 3 It is full of, continues to inject deionized water, the gas in PCR pipe is all discharged from outlet interface 5, it is anti-by micro- sem observation PCR It answers and whether there is bubble in room 3 in liquid.It tests for 10 times or more repeatedly, records experimental result such as table 1.

Table 1

From the aforegoing it can be seen that the invention, which can effectively avoid, generates the bubble that diameter is greater than 0.1mm, and it is less than the gas of 0.05mm Bubble influences less detection since size is less than fluorescence detection spot size.

Claims

1. A reagent reaction tube with a structure to avoid bubble generation, characterized in that it comprises a liquid inlet port (1), a liquid inlet conduit (2), a reaction chamber (3), an air outlet conduit (4) and an air outlet port (5) ; The liquid inlet conduit (2) is connected to the liquid inlet port (1) to the reaction chamber (3), and the gas outlet conduit (4) is connected to the reaction chamber (3) to the gas outlet port (5).

2 . The reagent reaction tube according to claim 1 , wherein the liquid inlet port ( 1 ) and the gas outlet port ( 5 ) are tubular structures protruding from the reagent reaction tube. 3 .

3 . The reagent reaction tube with a structure to avoid bubble generation according to claim 2 , wherein a tenon is provided in the middle of the liquid inlet port ( 1 ) and the gas outlet port ( 5 ). 4 .

4 . The reagent reaction tube with a structure to avoid bubble generation according to claim 1 , wherein the liquid inlet conduit ( 2 ) and the gas outlet conduit ( 4 ) are distributed in a U shape in the reagent reaction tube. 5 .

5. The reagent reaction tube according to claim 1, wherein the lower left corner of the reaction chamber (3) communicates with the liquid inlet conduit (2), and the upper right corner of the reaction chamber (3) The upper left corner and the lower right corner of the reaction chamber are designed with rounded corners in communication with the gas outlet conduit (4).

6 . The reagent reaction tube with a structure to avoid bubble generation according to claim 1 , wherein the side wall of the reagent reaction tube is sealed with a PVC film. 7 .