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CN109504603A - The detection method of total plate count in a kind of food - Google Patents

The detection method of total plate count in a kind of food Download PDF

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Publication number
CN109504603A
CN109504603A CN201811250875.1A CN201811250875A CN109504603A CN 109504603 A CN109504603 A CN 109504603A CN 201811250875 A CN201811250875 A CN 201811250875A CN 109504603 A CN109504603 A CN 109504603A
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China
Prior art keywords
total number
food
colonies
display
detection method
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CN201811250875.1A
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Chinese (zh)
Inventor
于佳禾
朱晓军
李洁莉
冯云
王丹丹
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Jiangsu Supervision and Inspection Institute for Product Quality
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Jiangsu Supervision and Inspection Institute for Product Quality
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

一种食品中菌落总数的检测方法主要涉及到食品检测技术领域，主要包括：反应室、MEMS感应芯片、传感装置、信号转化处理器和显示器。主要原理是根据微生物的呼吸作用产生的电子信号传递到MEMS感应芯片，由传感装置传递到信号转化处理器，转化成为数据或声音或者图像，通过显示器显示，其中本装置测试之前需要将已知食品中菌落总数的标准品和空白作为基准进行调试，可在10分钟之内获得样品的菌落总数，准确度在98％以上。本发明具有操作步骤简单、灵敏度高和周期短等特征。

A method for detecting the total number of bacterial colonies in food mainly relates to the technical field of food detection, and mainly includes: a reaction chamber, a MEMS sensing chip, a sensing device, a signal conversion processor and a display. The main principle is that the electronic signal generated by the respiration of microorganisms is transmitted to the MEMS sensor chip, which is transmitted by the sensor device to the signal conversion processor, converted into data or sound or image, and displayed on the display. The standard and blank of the total number of colonies in the food are used as benchmarks for debugging, and the total number of colonies in the sample can be obtained within 10 minutes, with an accuracy of over 98%. The invention has the characteristics of simple operation steps, high sensitivity and short period.

Description

The detection method of total plate count in a kind of food
Technical field
The invention belongs to field of food detection, a kind of detection method of total plate count in food is particularly belonged to.
Background technique
Food microorganisms pollution is to influence the major issue of food safety, wherein total plate count be the harm paid close attention to the most because One of element, total plate count refer to food by processing, under certain condition after culture, contained bacterium in gained 1g or 1mL sample Total plate count.Total plate count and all totals number of bacteria for indicating physical presence in sample, total plate count can not be distinguished wherein thin The type of bacterium, so sometimes referred to as miscellaneous bacteria number, aerobic bacterium colony etc..Total plate count method for quickly detecting in food common at present Including instant paper disk method, electrical impedance method, Microcolony count method, ATP bioluminescence method and near infrared spectroscopy etc..However, The problems such as there is the measurement period is long, data are unstable and detection formality is comparatively laborious.
Summary of the invention
In order to solve the above technical problems, the invention proposes a kind of technical sides of the detection method of the total element of bacterium colony in food Case specifically includes that reaction chamber, MEMS induction chip, sensing device, signal conversion processing device and display.Cardinal principle is root It is transmitted to MEMS induction chip according to the electronic signal that the respiration of microorganism generates, signal conversion is transmitted to by sensing device Device is managed, data or sound or image is transformed into, is shown by display, is wherein needed before present apparatus test by known food The standard items of total plate count and blank are debugged as benchmark in product, can obtain the total plate count of sample within ten minutes, Accuracy is 98% or more.
Further, reaction system and electrode are equipped in the reaction chamber, wherein reaction system various composition and its concentration is such as Shown in lower:
Neutral buffer: 40mM~49mM,
Matrix: 10mM-25mM,
Electronics amplification agent: 0.1mM-0.2mM.
Further, the MEMS induction chip is connected with electronic signal amplifying device.
Further, the sensing device mainly transmits the electric signal of chip acquisition, and is connected with electronics amplified signal device, Guarantee that signal repeatedly amplifies, convenient for identification.
Further, the display is that electronic display units, the display display modes such as mobile phone, computer are broadcast for sound Put or data output or image output etc..
Further, the detection method is as follows:
(1) it measures a certain amount of blank solution and carries out cleaning detection device, blank of going forward side by side calibration guarantees the accurate of benchmark Degree.
(2) reaction system is added in the microbial inoculum for measuring a certain amount of multiple known total plate counts respectively, establishes bacterium colony The standard curve of sum and voltage value.
(3) a certain amount of sample to be tested cloud slurries are taken to be added in reaction system, based on the standard curve having built up With the voltage value determined, display exports result.
Further, the sample to be tested homogenate is the quality hundred of sample to be tested and physiological saline according to 1:15~1:30 Divide than the homogenizing fluid after beating homogeneous after mixing.
The invention patent has following advantage compared with prior art: (1) in a kind of food total plate count detection Method is higher with sensitivity, the reaction time is short and operating process simple feature.(2) in a kind of food total plate count detection side Method uses intelligentized detection means, has the feature not vulnerable to factor interference and intelligent process, accuracy main road 98% More than.(3) detection means that the detection method of total plate count promotes China's food inspection industry in a kind of food is promoted.
Detailed description of the invention
The detection method of total plate count is related to device basic structure in a kind of food of Fig. 1
The detection method flow diagram of total plate count in a kind of food of Fig. 2；
In figure, 1- reaction chamber in Fig. 1,2-MEMS sensing chip, 3- sensing device, 4- signal conversion conversion processing device, 5- Display；201,202,203,204 and 205 part in Fig. 2.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiments of the present invention will be described in further detail.Implement below Example is not intended to limit the scope of the invention for the present invention.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " length ", " width ", " thickness ", The term positions such as "up" and "down".The fixation position of component is not implied that, it is thus impossible to enough be interpreted as to limit of the invention System.
Fig. 1 shows the preferred embodiment that the detection of total plate count in a kind of food according to the present invention is put.Such as figure It is shown, specifically include that reaction chamber (1), MEMS induction chip (2), sensing device (3), signal conversion processing device (4) and display (5).Cardinal principle is that the electronic signal generated according to the respiration of microorganism is transmitted to MEMS induction chip (2), by sensing Device (3) is transmitted to signal conversion processing device (4), transforms into data or sound or image, is shown by display (5), Wherein needs to debug using the standard items of total plate count in known food and blank as benchmark before present apparatus test, it can be The total plate count of sample is obtained within 10 minutes, accuracy is 98% or more.
Specifically, reaction system various composition and its concentration are as follows:
Neutral buffer: 40mM~49mM,
Matrix: 10mM-25mM,
Electronics amplification agent: 0.1mM-0.2mM.
The MEMS induction chip (2) is connected with electronic signal amplifying device.The sensing device (3) mainly transmits chip The electric signal of acquisition, and it is connected with electronics amplified signal device, guarantee that signal repeatedly amplifies, convenient for identification.The display is The electronic display units such as mobile phone, computer, display (5) display mode are that sound plays or data export or image output Deng.
The detection method is as follows:
(1) it measures a certain amount of blank solution (201) and carries out cleaning detection device, blank of going forward side by side calibration guarantees benchmark Accuracy.
(2) reaction system is added in the microbial inoculum (202) for measuring a certain amount of multiple known total plate counts respectively, establishes The standard curve (203) of total plate count and voltage value.
(3) a certain amount of sample to be tested cloud slurries are taken to be added in reaction system, based on the standard curve having built up With the voltage value (204) determined, display exports result (205).
The sample to be tested homogenate is that sample to be tested is mixed with physiological saline according to the mass percent of 1:15~1:30 The homogenizing fluid after homogeneous is patted afterwards.
The detection method of total plate count in a kind of food provided by the present invention, using sensing chip, the electricity in electrochemistry Pressure value is studied using Microorganism respiration as object, and the microorganism according to known total plate count draws total plate count and electricity The relation curve (204) of pressure value, can obtain result within ten minutes.
The foregoing is merely presently preferred embodiments of the present invention, can not limit the scope of the invention.

Claims (7)

1.一种食品中菌落总数的检测方法，其特征在于，主要包括：反应室、MEMS感应芯片、传感装置、信号转化处理器和显示器。主要原理是根据微生物的呼吸作用产生的电子信号传递到MEMS感应芯片，由传感装置传递到信号转化处理器，转化成为数据或声音或者图像，通过显示器显示，其中本装置测试之前需要将已知食品中菌落总数的标准品和空白作为基准进行调试，可在10分钟之内获得样品的菌落总数，准确度在98％以上。1. the detection method of total number of bacterial colonies in food, is characterized in that, mainly comprises: reaction chamber, MEMS induction chip, sensing device, signal conversion processor and display. The main principle is that the electronic signal generated by the respiration of microorganisms is transmitted to the MEMS sensor chip, which is transmitted by the sensor device to the signal conversion processor, converted into data or sound or image, and displayed on the display. The standard and blank of the total number of colonies in the food are used as benchmarks for debugging, and the total number of colonies in the sample can be obtained within 10 minutes, with an accuracy of over 98%. 2.根据权利要求1所述的一种食品中菌落总数的检测方法，其特征在于，所述反应室内设有反应体系和电极，其中反应体系各种成分及其浓度如下所示：2. the detection method of total number of bacterial colonies in a kind of food according to claim 1, is characterized in that, described reaction chamber is provided with reaction system and electrode, and wherein various components of reaction system and concentration thereof are as follows: 中性缓冲溶液：40mM～49mM,Neutral buffer solution: 40mM～49mM, 基质：10mM-25mM,Matrix: 10mM-25mM, 电子放大剂：0.1mM-0.2mM。Electron amplifier: 0.1mM-0.2mM. 3.根据权利要求1所述的一种食品中菌落总数的检测方法，其特征在于，所述MEMS感应芯片连接有电子信号放大装置。3 . The method for detecting the total number of bacterial colonies in a food according to claim 1 , wherein the MEMS sensing chip is connected with an electronic signal amplifying device. 4 . 4.根据权利要求1所述的一种食品中菌落总数的检测方法，其特征在于，所述传感装置主要传递芯片采集的电信号，并连接有电子放大信号装置，保证信号多次放大，便于识别。4. The method for detecting the total number of bacterial colonies in a food according to claim 1, wherein the sensing device mainly transmits the electrical signal collected by the chip, and is connected with an electronic amplifying signal device to ensure that the signal is repeatedly amplified, easy to identify. 5.根据权利要求1所述的一种食品中菌落总数的检测方法，其特征在于，所述显示器为手机、电脑等电子显示设备，所述显示器显示方式为声音播放或数据输出或图像输出等。5. the detection method of total number of bacterial colonies in a kind of food according to claim 1, is characterized in that, described display is electronic display equipment such as mobile phone, computer, and described display mode is sound playback or data output or image output etc. . 6.根据权利要求1所述的一宗食品中菌落总数的检测方法，其特征在于，所述检测方法如下所示：6. the detection method of total number of bacterial colonies in a food according to claim 1, is characterized in that, described detection method is as follows: (1)量取一定量的空白溶液进行清洗检测装置，并进空白校准，保证基准的准确度。(1) Measure a certain amount of blank solution to clean the detection device, and perform blank calibration to ensure the accuracy of the benchmark. (2)分别量取一定量的多个已知菌落总数的微生物菌液加入反应体系，建立菌落总数和电压值的标准曲线。(2) Measure a certain amount of microbial liquids with known total number of colonies, respectively, and add them to the reaction system to establish a standard curve of total number of colonies and voltage values. (3)取一定量的待测样品云浆液加入反应体系中，基于已经建立起来的标准曲线和测定出的电压值，显示器输出结果。(3) Take a certain amount of the cloud slurry of the sample to be tested and add it to the reaction system. Based on the established standard curve and the measured voltage value, the display outputs the result. 7.根据权利要求6所述的一种食品中菌落总数的检测方法，其特征在于，所述待测样品匀浆液为待测样品与生理盐水按照1:15～1:30的质量百分比混合后拍打均质后的均质液。7. The method for detecting the total number of bacterial colonies in a food according to claim 6, wherein the homogenate of the sample to be tested is after the sample to be tested and the physiological saline are mixed in a mass percentage of 1:15～1:30 Beat the homogenized solution.
CN201811250875.1A 2018-10-25 2018-10-25 The detection method of total plate count in a kind of food Pending CN109504603A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115184285A (en) * 2022-06-28 2022-10-14 佳木斯大学 A device and method for rapidly determining the total number of bacteria based on multi-wavelength reflectance spectroscopy

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1440454A (en) * 2000-05-03 2003-09-03 高振智 Biological identification system with integrated sensor chip
US20130266977A1 (en) * 2010-02-16 2013-10-10 President And Fellows Of Harvard College Methods and systems for detection of microbes
CN107843624A (en) * 2017-09-14 2018-03-27 中国肉类食品综合研究中心 For detecting the detection means and its detection method of total plate count in food

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1440454A (en) * 2000-05-03 2003-09-03 高振智 Biological identification system with integrated sensor chip
US20130266977A1 (en) * 2010-02-16 2013-10-10 President And Fellows Of Harvard College Methods and systems for detection of microbes
CN107843624A (en) * 2017-09-14 2018-03-27 中国肉类食品综合研究中心 For detecting the detection means and its detection method of total plate count in food

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115184285A (en) * 2022-06-28 2022-10-14 佳木斯大学 A device and method for rapidly determining the total number of bacteria based on multi-wavelength reflectance spectroscopy

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Application publication date: 20190322