CN109481070B - Implantable internal carotid artery constant-speed injection device and operation method thereof - Google Patents

Abstract

The invention discloses an implantable internal carotid artery constant-speed injection device and an operation method thereof. The invention adopts a sealing cover and a sealing plug which are respectively arranged at the top end and the rear end of a carotid artery implantation catheter, namely the sealing cover and the sealing plug can be penetrated by a needle, an injection opening covered by a unidirectional flow gate is arranged at the front end of the carotid artery implantation catheter, the flow speed and the time are controlled by a controller, a constant-speed injection micropump is driven to push an injector, and cancer cells or injection medicines flow into a carotid artery vessel through the injection opening of the carotid artery implantation catheter; the invention can simplify the operation method, simplify the operation steps and shorten the operation time; meanwhile, the operation mode of the animal model injected into the internal carotid artery of the mouse is completely changed, the external carotid artery of the mouse is not required to be sutured, the vagus nerve and the blood vessel are not damaged, and the success rate of the operation is greatly improved.

Description

An implantable internal carotid artery constant-velocity injection device and its operating method

technical field

The invention relates to mouse animal model technology, in particular to an implantable internal carotid artery constant-velocity injection device and an operating method thereof.

Background technique

When making mouse animal models of brain metastasis of various cancer cells, it is usually necessary to ligate the proximal end of the common carotid artery and the external carotid artery of the mouse, and then use a syringe to inject cancer cells cultured in vitro from the distal end of the common carotid artery. The needle is injected into the internal carotid artery to reach the blood-brain barrier, and then spontaneously transferred to the brain to create a mouse animal model most similar to human cancer brain metastasis. According to the local anatomy, the common carotid artery is divided forward into the external carotid artery and the internal carotid artery, and only the blood in the internal carotid artery flows into the cranium. Therefore, only by ligation of the external carotid artery can the blood flow in the common carotid artery flow forward into the internal carotid artery. Since mouse surgery needs to be performed under a microscope, the surgical operation must be very delicate. The most critical step in the surgical process is the ligation of the external carotid artery of the mouse, and then the cancer cells can be completely injected into the internal carotid artery and transferred to the brain with a syringe. When suturing the external carotid artery, care must be taken to protect the accompanying vagus nerve and venous vessels from damage, otherwise it will cause cardiac arrest and blood loss in mice, which is an important reason for the death of mice with failed surgery. In addition, after successful ligation of the external carotid artery, the needle of the syringe filled with cancer cells needs to be inserted into the distal end of the common carotid artery (above the ligation line at the proximal end), and then the cancer cells cultivated in vitro are injected into the internal carotid artery. The process needs to strictly control the injection speed, that is, when the concentration of cancer cells is constant, the faster the injection speed, the more cancer cells will reach the blood-brain barrier per unit time, which will artificially cause tumor embolus and block blood vessels, which is equivalent to causing small Mice suffered from extensive thrombotic disease and died, which is another important reason for the death of mice with failed surgery.

Contents of the invention

Aiming at the above problems in the prior art, the present invention proposes an implantable constant-velocity injection device for the internal carotid artery and its operation method, so as to improve the success rate of making mouse animal models and reduce the death rate of mice.

One object of the present invention is to provide an implantable internal carotid artery constant velocity injection device.

The implantable internal carotid artery constant-velocity injection device of the present invention comprises: a common carotid artery implant catheter, a sealing cap, a sealing plug, an injection opening, a one-way flow door, a marker, a needle, a syringe, a pusher, and a constant-velocity injection micro-injection device. Pump and controller; wherein, the common carotid artery implanted catheter is a hollow cylindrical hollow catheter; the top of the common carotid artery implanted catheter is provided with a sealing cover, and the rear end is provided with a sealing plug; An injection opening is opened on the side wall of the front end of the catheter; a one-way flow gate is arranged at the injection opening and outside the common carotid artery implant catheter to cover the injection opening, and the front end of the one-way flow gate is connected to the common carotid artery implant catheter On the side wall of the front end of the common carotid artery implantation catheter, the one-way flow door can be opened and closed around the joint; the front end of the common carotid artery implantation catheter is provided with markers; the common carotid artery implantation catheter, the sealing cap , a sealing plug, an injection opening, a one-way flow gate and markers constitute a common carotid artery implantation device; the front end of the syringe is connected to a needle, and the shape of the needle is a broken line with an obtuse angle; the needle penetrates the sealing plug and is implanted deep into the common carotid artery In the catheter; the piston handle at the bottom of the syringe is connected to the pusher; the pusher is connected to the constant-speed injection micropump; the constant-speed injection micropump is connected to the controller; the empty barrel of the syringe is filled with injection, and the injection rate is set by the controller , control the constant-speed injection micropump to drive the propeller, push the piston handle of the syringe, inject the injection into the common carotid artery implant catheter through the needle, and open the one-way flow door through the injection opening to flow out; after the injection, due to blood backlash The pressure of the flow is stronger than the pressure in the catheter implanted in the common carotid artery, that is, the catheter implanted in the common carotid artery is under negative pressure, and the one-way flow gate is closed.

Before the operation, the common carotid artery implant catheter is inserted into the common carotid artery vessel, and the common carotid artery implant catheter is removed after the operation.

Common carotid artery catheters are made of polyvinyl chloride PVC, polyethylene PE or polystyrene PS. The inner diameter of the catheter implanted in the common carotid artery is 0.5-0.75 mm; the outer diameter is 1-1.25 mm.

The material of the sealing cover and the sealing plug is one of rubber, latex and silica gel.

The diameter of the injection opening is 1-2 mm, and the distance from the top of the catheter implanted in the common carotid artery is 3-4 mm. The present invention uses a sealing cap to block the top of the common carotid artery implantation catheter, and sets an injection opening on the side wall of the common carotid artery implantation catheter, so that all the injections are injected into the internal carotid artery, thus eliminating the need for suturing mice The external carotid artery will not damage the vagus nerve and blood vessels.

A one-way flow door is set outside the injection opening. During the injection process, the injection can open the one-way flow door and flow out. After the injection is completed, due to the negative pressure in the common carotid artery implant catheter, the one-way flow door is closed and covered. The injection opening is blocked to prevent backflow of blood in the internal carotid artery into the common carotid artery implant catheter.

The marker is an indicator strip, and the surface of the indicator strip is coated with a fluorescent agent; the indicator strip is tightly adhered to the inner surface of the sealing cover.

The obtuse angle of the needle is 120-145°, which not only facilitates the puncture operation of the common carotid artery, but also facilitates the implantation of the common carotid artery catheter, and the common carotid artery of the mouse will not be damaged during the injection operation.

The syringe includes an empty barrel, a piston and a piston handle; a connected needle is arranged at the top of the empty barrel, a piston handle is arranged at the bottom of the piston, and the front end of the piston goes deep into the empty barrel from the bottom of the empty barrel.

The propeller includes a push head and a lead screw; wherein, the front end of the push head is connected to the piston handle of the syringe, the rear end is connected to the lead screw, and the lead screw is connected to a constant-speed injection micropump. The constant-speed injection micropump is controlled by the controller. The control signal of the controller drives the constant-speed injection micropump, which drives the screw to rotate. Movement, thereby pushing the plunger of the syringe at the front end of the push head to inject cancer cells or drugs.

The controller includes a power supply, an input device, an output device and a control circuit board; wherein the power supply is connected to the input device, the output device and the control circuit board respectively; the control circuit board is respectively connected to the input device and the output device; the infusion is set through the input device Speed, injection time and injection volume, the output device adopts a display screen, and the display screen is a liquid crystal display LCD. By setting the infusion speed and injection time of the constant-speed injection micropump and then controlling the rotation speed of the screw, the forward speed of the plunger of the syringe can be controlled.

The dose of the injection is 500-200 μl; the constant injection rate is 0.50-1.0 μl/s, and the injection time is 100-200s.

Further, the present invention also includes a protective sheath, which is sheathed outside the common carotid artery implantation device.

The injections are various cancer cells metastasized to the brain or various injected drugs for treatment.

Another object of the present invention is to provide an operation method of an implantable internal carotid artery constant-velocity injection device.

The operation method of the implantable internal carotid artery constant velocity injection device of the present invention comprises the following steps:

1) Fill the injection in the syringe;

2) Connect the plunger handle at the bottom end of the syringe to the pusher;

3) Connect the propeller to a constant-speed injection micropump;

4) Connect the constant-speed injection micropump to the controller;

5) The needle with an obtuse angle penetrates the sealing plug deeply into the common carotid artery implant catheter; then penetrates the sealing cover to expose the needle tip;

6) Return the needle tip to the common carotid artery implantation catheter;

7) Set the injection rate through the controller, control the constant-speed injection micropump to drive the propeller, push the piston handle of the syringe, inject the injection through the needle into the common carotid artery implant catheter, and open the one-way flow through the injection opening door flow;

8) After the injection, due to the negative pressure in the common carotid artery implant catheter, the one-way flow door is closed.

Advantages of the present invention:

In the present invention, a sealing cover and a sealing plug that are sealed and can be pierced by a needle are respectively arranged at the top and rear ends of the common carotid artery implant catheter, and an injection valve covered by a one-way flow door is arranged at the front end of the common carotid artery implant catheter. Opening, the flow rate and time are controlled by the controller, and the constant-speed injection micropump is driven to push the syringe, so that the cancer cells or injected drugs flow into the common carotid artery through the injection opening of the common carotid artery implant catheter; the invention can simplify and simplify the operation At the same time, it will completely change the surgical method of the mouse internal carotid artery injection animal model, without suturing the external carotid artery of the mouse, and will not damage the vagus nerve and blood vessels, greatly improving the success rate of the operation.

Description of drawings

Fig. 1 is the schematic diagram of an embodiment of common carotid artery implantation device of the present invention;

Fig. 2 is a schematic diagram before use of an embodiment of the implantable internal carotid artery constant-velocity injection device of the present invention;

Fig. 3 is a schematic diagram of connection in use of an embodiment of the implantable internal carotid artery constant-velocity injection device of the present invention.

Detailed ways

The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

As shown in Figures 1 and 2, the implantable internal carotid artery constant-velocity injection device of this embodiment includes: common carotid artery implant catheter 1, sealing cap 2, sealing plug 3, injection opening, one-way flow door 4, marking object, needle 5, syringe 6, protective cover 7, propeller, constant-speed injection micropump and controller; wherein, the common carotid artery implant catheter 1 is a hollow cylindrical hollow catheter; the common carotid artery implant catheter The top end of 1 is provided with a sealing cap 2, and the rear end is provided with a sealing plug 3; an injection opening is opened on the side wall of the front end of the common carotid artery implantation catheter 1; at the injection opening and outside the common carotid artery implantation catheter 1 A one-way flow door is provided to cover the injection opening. The front end of the one-way flow door is connected to the side wall of the front end of the common carotid artery implant catheter 1, and the one-way flow door can be placed on the common carotid artery implant catheter 1 around the joint. External opening and closing; the front end of the common carotid artery implant catheter 1 is provided with markers; the common carotid artery implant catheter 1, sealing cap 2, sealing plug 3, injection opening, one-way flow door 4 and markers constitute the common carotid artery Implantation device; the protective sleeve 7 is set outside the common carotid artery implantation device; the front end of the syringe 6 is connected to the needle 5, and the shape of the needle 5 is a broken line with an obtuse angle; the needle 5 penetrates the sealing plug 3 and is implanted deep into the common carotid artery Inside the conduit 1; the piston handle at the bottom of the syringe 6 is connected to the propeller; the propeller is connected to the constant-speed injection micropump; the constant-speed injection micropump is connected to the controller; the empty barrel of the syringe 6 is filled with the injection, which is set by the controller The injection rate is controlled by a constant-speed injection micropump to drive the propeller, push the piston handle of the syringe 6, inject the injection through the needle 5 into the common carotid artery implantation catheter 1, pass through the injection opening, and open the one-way flow door to flow out; injection After the end, because the pressure of blood reflux is stronger than the pressure in the common carotid artery implantation catheter 1, that is, the common carotid artery implantation catheter 1 is under negative pressure, and the one-way flow door is closed. The syringe 6 includes an empty barrel 61 , a piston 62 and a piston handle 63 .

In the present embodiment, the common carotid artery implant catheter 1 adopts polyvinyl chloride PVC; the material of the sealing cover 2 and the sealing plug 3 adopts rubber; the diameter of the injection opening is 1mm, and the distance from the top of the common carotid artery implant catheter 1 is 3mm. The marker is an indicator strip, the surface of the indicator strip is coated with a fluorescent agent and tightly adhered to the inner surface of the sealing cap 2; the obtuse angle of the needle 5 is 135°; the dose of the injection is 100 μl; the constant injection rate is 0.50 μl/s, The injection time is between 100-200s.

The implantable internal carotid artery constant-velocity injection device of this embodiment is used in the operation of mouse internal carotid artery injection, as shown in Figure 3, the operation method includes the following steps:

1) filling the syringe 6 with cancer cells or medicine;

2) Connect the piston handle at the bottom end of the syringe 6 to the propeller;

3) Connect the propeller to a constant-speed injection micropump;

4) Connect the constant-speed injection micropump to the controller;

5) Tear off the protective cover 7, embed the common carotid artery implant device into the common carotid artery vessel wall, penetrate the sealing plug 3 with the needle 5 having an obtuse angle of 135° and penetrate deeply into the common carotid artery implant catheter 1; The needle tip is exposed through the sealing cover 2;

6) Puncture the vessel wall of the common carotid artery with the needle tip, and immediately return the needle tip to the common carotid artery implantation catheter 1;

7) Set the injection rate through the controller, control the constant-speed injection micropump to drive the propeller, push the piston handle of the syringe 6, inject the injection into the common carotid artery implant catheter 1 through the needle 5, and flush through the injection opening One-way flow door outflow;

8) After the injection, due to the negative pressure in the common carotid artery implant catheter 1, the one-way flow door is closed;

9) After the operation, the common carotid artery implant device was removed.

Finally, it should be noted that the purpose of the disclosed embodiments is to help further understand the present invention, but those skilled in the art can understand that various replacements and modifications can be made without departing from the spirit and scope of the present invention and the appended claims. It is possible. Therefore, the present invention should not be limited to the content disclosed in the embodiments, and the protection scope of the present invention is subject to the scope defined in the claims.

Claims (6)

1. An implantable internal carotid artery constant-speed injection device, comprising: the common carotid artery implantation catheter, the sealing cover, the sealing plug, the injection opening, the unidirectional flow gate, the marker, the needle, the injector, the propeller, the constant-speed injection micro pump and the controller; wherein, the common carotid artery implantation catheter is a cylindrical hollow catheter with hollow inside; the top end of the carotid artery implanted catheter is provided with a sealing cover, and the rear end is provided with a sealing plug; an injection opening is arranged on the side wall of the front end of the carotid artery implantation catheter; a unidirectional flow gate is arranged at the injection opening and positioned outside the carotid artery implantation catheter and covers the injection opening, the front end of the unidirectional flow gate is connected to the side wall of the front end of the carotid artery implantation catheter, and the unidirectional flow gate can be opened and closed outside the carotid artery implantation catheter around the connection position; a marker is arranged at the front end of the common carotid artery implantation catheter; the common carotid artery implantation catheter, the sealing cover, the sealing plug, the injection opening, the unidirectional flow gate and the marker form a common carotid artery implantation device; the front end of the injector is connected with a needle head, the shape of the needle head is a folded line type with an obtuse angle, and the obtuse angle of the needle head is 120-145 degrees; the needle penetrates the sealing plug and goes deep into the carotid artery implantation catheter; the piston handle at the bottom end of the injector is connected to the propeller; the propeller is connected to a constant-speed injection micro pump; the constant-speed injection micropump is connected to the controller; embedding the common carotid artery implant device into the wall of the common carotid artery vessel, penetrating the needle head through the sealing plug and deep into the common carotid artery implant catheter, and penetrating the sealing cover to expose the needle point; puncturing the wall of the carotid artery vessel by using the needle point, and immediately returning the needle point to the carotid artery for implantation in the catheter; setting the injection rate through a controller, controlling a constant-speed injection micro pump to drive a propeller, pushing a piston handle of the injector, and injecting the injection into the common carotid artery implantation catheter through a needle; the empty cylinder of the injector is filled with the injection, the injection rate is set by the controller, the constant-speed injection micro pump is controlled to drive the propeller, the piston handle of the injector is pushed, the injection is injected into the carotid artery implantation catheter through the needle, and the injection flows out through the injection opening and the one-way flow gate; after the injection is finished, the pressure of the blood reflux is higher than the pressure in the carotid artery implanted catheter, namely the carotid artery implanted catheter is negative pressure, and the unidirectional flow gate is closed; the device also comprises a protective sleeve which is sleeved outside the common carotid artery implantation device.

2. The constant velocity injection device of claim 1, wherein the common carotid artery implant catheter is polyvinyl chloride PVC, polyethylene PE or polystyrene PS; the internal diameter of the common carotid artery implantation catheter is 0.5-0.75 mm; the outer diameter is 1-1.25 mm.

3. The constant velocity injection device of claim 1, wherein the sealing cap and sealing plug are made of one of rubber, latex and silicone.

4. The constant velocity injection device of claim 1, wherein the injection opening has a diameter of 1 to 2mm and a distance of 3 to 4mm from the tip of the common carotid artery implantation catheter.

5. The constant velocity injection device of claim 1, wherein the marker employs an indicator strip, the indicator strip surface being coated with a fluorescent agent; the indicator strip is tightly adhered to the inner surface of the sealing cover.

6. The constant velocity injection device of claim 1, wherein the controller comprises a power source, an input device, an output device, and a control circuit board; wherein, the power supply is respectively connected to the input device, the output device and the control circuit board; the control circuit board is respectively connected to the input device and the output device; the infusion rate and injection time are set by the input device.

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