CN109453429A - One kind prevents adhesion anti-infectious hernia photo fix and preparation method thereof - Google Patents
One kind prevents adhesion anti-infectious hernia photo fix and preparation method thereof Download PDFInfo
- Publication number
- CN109453429A CN109453429A CN201811527525.5A CN201811527525A CN109453429A CN 109453429 A CN109453429 A CN 109453429A CN 201811527525 A CN201811527525 A CN 201811527525A CN 109453429 A CN109453429 A CN 109453429A
- Authority
- CN
- China
- Prior art keywords
- hernia
- parts
- photo fix
- high polymer
- polymer material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 206010019909 Hernia Diseases 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 230000002924 anti-infective effect Effects 0.000 title claims abstract description 18
- 239000000835 fiber Substances 0.000 claims abstract description 25
- 229920001661 Chitosan Polymers 0.000 claims abstract description 24
- 239000004132 Calcium polyphosphate Substances 0.000 claims abstract description 23
- 235000019827 calcium polyphosphate Nutrition 0.000 claims abstract description 23
- 239000002861 polymer material Substances 0.000 claims abstract description 22
- 239000002131 composite material Substances 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 239000012462 polypropylene substrate Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 239000002904 solvent Substances 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 15
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 150000007524 organic acids Chemical class 0.000 claims description 11
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 9
- 239000004626 polylactic acid Substances 0.000 claims description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 8
- 239000003960 organic solvent Substances 0.000 claims description 8
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 206010021620 Incisional hernias Diseases 0.000 claims description 4
- 229920001244 Poly(D,L-lactide) Polymers 0.000 claims description 4
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 3
- 208000027536 Femoral Hernia Diseases 0.000 claims description 2
- 239000003519 biomedical and dental material Substances 0.000 claims description 2
- 206010045458 umbilical hernia Diseases 0.000 claims description 2
- 125000004494 ethyl ester group Chemical group 0.000 claims 1
- 210000004013 groin Anatomy 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 9
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 238000001727 in vivo Methods 0.000 abstract description 5
- 210000001519 tissue Anatomy 0.000 description 23
- 241000700159 Rattus Species 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 11
- 102000008186 Collagen Human genes 0.000 description 9
- 108010035532 Collagen Proteins 0.000 description 9
- 229920001436 collagen Polymers 0.000 description 9
- 102000001187 Collagen Type III Human genes 0.000 description 8
- 108010069502 Collagen Type III Proteins 0.000 description 8
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 5
- 230000002093 peripheral effect Effects 0.000 description 5
- 229920001155 polypropylene Polymers 0.000 description 5
- 206010060954 Abdominal Hernia Diseases 0.000 description 4
- 102000002734 Collagen Type VI Human genes 0.000 description 4
- 108010043741 Collagen Type VI Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 210000001835 viscera Anatomy 0.000 description 4
- 208000021970 Abdominal wall defect Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 125000003342 alkenyl group Chemical group 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 206010033675 panniculitis Diseases 0.000 description 3
- -1 polypropylene Polymers 0.000 description 3
- 230000002980 postoperative effect Effects 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 210000004304 subcutaneous tissue Anatomy 0.000 description 3
- 230000008467 tissue growth Effects 0.000 description 3
- 206010000050 Abdominal adhesions Diseases 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 102000012422 Collagen Type I Human genes 0.000 description 2
- 108010022452 Collagen Type I Proteins 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 206010016717 Fistula Diseases 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000035563 Postoperative hernia Diseases 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 239000000560 biocompatible material Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 230000003890 fistula Effects 0.000 description 2
- 230000005661 hydrophobic surface Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000010148 water-pollination Effects 0.000 description 2
- YEJRWHAVMIAJKC-UHFFFAOYSA-N 4-Butyrolactone Chemical compound O=C1CCCO1 YEJRWHAVMIAJKC-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000248349 Citrus limon Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 208000029836 Inguinal Hernia Diseases 0.000 description 1
- 208000008081 Intestinal Fistula Diseases 0.000 description 1
- 208000004550 Postoperative Pain Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000035091 Ventral Hernia Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 210000003815 abdominal wall Anatomy 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical group O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 229920000295 expanded polytetrafluoroethylene Polymers 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003195 fascia Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000003871 intestinal function Effects 0.000 description 1
- 208000003243 intestinal obstruction Diseases 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229920001610 polycaprolactone Polymers 0.000 description 1
- 239000004632 polycaprolactone Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/06—Coatings containing a mixture of two or more compounds
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to biomedical materials fields, and in particular to one kind prevents adhesion anti-infectious hernia patch and preparation method thereof.The hernia photo fix is mainly made of polypropylene substrate and composite membrane, and the composite membrane is mainly made of following raw material and its parts by weight: 30~60 parts of degradable high polymer material, 20~50 parts of chitosan and 2~10 parts of calcium polyphosphate fiber.Hernia patch of the invention avoids polypropylene substrate and directly contacts with tissue and lead to the problem of serious adhesion, and the compound film raw material can slowly degrade in vivo, good biocompatibility, have significant anti-infectious function, it can also stimulate to form mature and stable collagenous tissue, reduce recurrence rate.
Description
Technical field
The invention belongs to biomedical materials fields, and in particular to one kind prevents adhesion anti-infectious hernia photo fix and its preparation side
Method.
Background technique
Hernia is that some internal organs or tissue leave its normal anatomy position in human body, the weakness formed by the congenital or day after tomorrow
Point, defect or hole enter another position, and the various ages may occur, and there are easy to recur, repairings for traditional method for repairing and mending
The disadvantages of time is long, postoperative pain, replaced the tension-free hernioplasty for being gradually used sticking patch mode.Tension-free hernioplasty
Method are as follows: first hernia occur position upper opening, the internal organs squeezed into the position are pushed back in abdominal cavity, then will be by biology
Compatibility material is placed in the opening and is secured in position it by suture or other modes, is then shut off the opening, subtracts
The deformation that opening tissue around is born less, makes opening gradually heal.As it can be seen that biocompatible materials employed in herniorrhaphy
It is most important.
In the prior art, in tension-free hernioplasty be commonly used biocompatible materials include PP type sticking patch,
Polyesters sticking patch, Expanded Polytetrafluoroethylene etc..Wherein, PP type sticking patch mesh is big, be conducive to surrounding tissue grow into and
The immersion of macrophage is not easy in twine to hide bacterium, has preferable resistance infection and maintenance performance, and application is relatively broad,
But polypropylene material quality is harder, can lead to more serious intestinal adhesion and influences gut function, even results in intestinal obstruction and intestinal fistula,
It cannot directly be contacted with intestinal tube when use；Polyesters sticking patch flexibility is good, but tension stress ability is lower；Expanded PTFE is mended
Piece is microporosity biomaterial, is not easy to be formed adhesion with abdominal viscera when contacting, but its micropore face be not easy to fibroblast and
Macrophage enters, so anti-infection ability is lower.
Chinese patent application CN102058442A discloses a kind of hernia patch and preparation method thereof of compound anti-adhesion, institute
State that hernia patch includes propene polymer patch and expanded polytetrafluoroethylsealing prevents adhesion sticking patch, the expanded polytetrafluoroethylsealing prevents adhesion benefit
Piece is compounded on propene polymer patch.The hernia patch has taken into account the advantages of polypropylene and expanded polytetrafluoroethylsealing material, can prevent
Intestinal adhesion, stationarity are good, tension stress is strong, but the anti-infection ability of expanded polytetrafluoroethylsealing is still without improvement.
Chinese patent application CN107837420A discloses a kind of preparation method for caning absorb anti-infective hernia patch, described
Hernia patch includes polypropylene substrate, polypropylene substrate peripheral hardware is equipped with by degradable high polymer material, chitosan solution is compound and
At composite membrane, the hernia patch of the preparation can prevent internal organ and patch adhesion, reduce complication rate, but wherein
Degradable high polymer material be polylactic acid, hydrophily is poor, weak to the adhesiveness of cell, while its catabolite in vivo
For lactic acid, although water can be degraded by tricarboxylic acid cycle in vivo and carbon dioxide excretes, its product slant acidity,
Be unfavorable for cell growth be also possible to aggregation cause internal inflammatory reaction, be easy recurrence.
Therefore, there is an urgent need to develop a kind of promotion tissue cell growth, it is anti-infective, prevent adhesion, the repairing of hernia that recurrence rate is low
Piece and preparation method thereof.
Summary of the invention
The present invention is intended to provide a kind of promotion tissue cell growth, it is anti-infective, prevent adhesion, the hernia patch that recurrence rate is low and
Preparation method.
In order to achieve the above object, the invention adopts the following technical scheme:
One kind prevents adhesion anti-infectious hernia photo fix, and the hernia photo fix is mainly made of polypropylene substrate and composite membrane, described
Composite membrane is mainly made of following raw material and its parts by weight: 30~60 parts of degradable high polymer material, 20~50 parts of chitosan
With 2~10 parts of calcium polyphosphate fiber.
Further, the composite membrane is mainly made of following raw material and its parts by weight: degradable high polymer material 50
~60 parts, 35~50 parts of chitosan and 5~10 parts of calcium polyphosphate fiber.
Further, the composite membrane is mainly made of following raw material and its parts by weight: degradable high polymer material
50 parts, 45 parts of chitosan and 5 parts of calcium polyphosphate fiber.
Further, the degradable high polymer material is selected from one of l-lactic acid or poly-dl-lactide or two
Kind.
Further, the relative molecular weight of the degradable high polymer material is 100,000~400,000.
Further, the preparation method of the hernia photo fix, comprising the following steps:
S1, take the degradable high polymer material of corresponding amount be dissolved in w/v be 1:(5~20) organic solvent in,
Stirring makes it completely dissolved under confined conditions, and ultrasonic deaeration obtains mixed liquor A；
S2, take the chitosan of corresponding amount and calcium polyphosphate fiber be dissolved in w/v be 1:(5~20) organic acid
In solvent, stirring is made it completely dissolved under confined conditions, and ultrasonic deaeration obtains mixed liquid B；
S3, mixed liquor A obtained by step S1 is mixed with mixed liquid B obtained by step S2, stirs evenly, is poured into and is covered with poly- third
In the mold of alkenyl piece, evaporation solvent at film to get.
Further, the organic solvent in the step S1 is selected from acetone, ethyl acetate, chloroform and tetrahydrofuran
One of.
Further, the organic acid solvent in the step S2 be 0.35~0.7mol/L of concentration acetic acid or 0.4~
The citric acid of 0.6mol/L.
In addition, the present invention also provides the preparation methods of the hernia photo fix, comprising the following steps:
I, take the degradable high polymer material of corresponding amount be dissolved in w/v be 1:(5~20) organic solvent in,
The organic solvent is selected from one of acetone, ethyl acetate, chloroform and tetrahydrofuran, and stirring keeps its complete under confined conditions
Fully dissolved, ultrasonic deaeration, obtains mixed liquor A；
II, take the chitosan of corresponding amount and calcium polyphosphate fiber be dissolved in w/v be 1:(5~20) organic acid
In solvent, the organic acid solvent is the acetic acid of 0.35~0.7mol/L of concentration or the citric acid of 0.4~0.6mol/L, closed item
Stirring makes it completely dissolved under part, and ultrasonic deaeration obtains mixed liquid B；
III, mixed liquor A obtained by step I is mixed with mixed liquid B obtained by step II, stirs evenly, is poured into and is covered with poly- third
In the mold of alkenyl piece, evaporation solvent at film to get.
In addition, the present invention also provides the hernia photo fix or the power preparation method preparation treatment direct inguinal hernia,
Indirect hernia, femoral hernia, umbilical hernia, hernia of white line or incisional hernia bio-medical material in application.
Composite membrane in the present invention is prepared as a raw material with polylactic acid, chitosan and calcium polyphosphate fiber, is had good
Biocompatibility, can slowly degrade in vivo.Applicant's pleasantly surprised discovery in practice, hernia patch prepared by the present invention
It can promote tissue cell growth, significantly reduce recurrence rate.It is lacked in rat stomach wall for 1 group of the embodiment of the present invention it can be seen from test 1
The the 6th, 8 week I/III Collagen Type VI ratio after damage prosthesis is maintained at 4:1 or so, and stimulation forms mature and stable collagenous fibres
Tissue reduces recurrence rate.It may be a certain amount of chitosan and calcium polyphosphate fiber because of addition, on the one hand, hydrophily
The presence of chitosan and calcium polyphosphate fiber can improve the hydrophobic surface of polylactic acid, and the hydrophobic surface of polylactic acid
Too fast infiltration of the moisture to chitosan and calcium polyphosphate fiber can be slowed down, slow down the degradation speed of the two；On the other hand, shell is poly-
The catabolite of sugar and calcium polyphosphate fiber can reduce the influence that acid product grows cell, reduce the generation of inflammatory reaction,
Collective effect promotes tissue cell growth, forms mature and stable collagenous tissue, reduces postoperative recurrence.
In addition, polylactic acid is easy to produce hydrolysis, Shen when mixing with chitosan due to the presence of water in organic acid solvent
Ask someone to be found surprisingly that in practice, chitosan is first crosslinked with calcium polyphosphate fiber, and the two can lock moisture, then with polylactic acid
Mixing, can significantly reduce the degradation situation of polylactic acid.Three is arranged outside polypropylene substrate with centainly matching manufactured composite membrane,
Contacted with body tissue organ can be used as cell growth depend on but not generate adhesion, avoid polypropylene substrate and tissue directly
Contact leads to the problem of serious adhesion.Hernia patch of the present invention can extraordinary reparation rat abdomen it can be seen from test example 1
Wall defect, postoperative hernia patch surrounding tissue soft-touch, without adhesion phenomenon, tissue growth is all right, a large amount of fibers
Tissue encapsulation sticking patch, thickness is thicker, does not have the case where infecting.
The invention has the following advantages that
(1) one kind of the present invention prevents adhesion anti-infectious hernia patch, has set one layer of composite membrane in polypropylene substrate peripheral hardware, has kept away
Exempt from polypropylene substrate directly to contact with tissue and lead to the problem of serious adhesion, and the compound film raw material can be slow in vivo
Degradation, good biocompatibility have significant anti-infectious function.
(2) a kind of composite membrane for the anti-infectious hernia patch that prevents adhesion of the present invention is with polylactic acid, chitosan and calcium polyphosphate
Fiber is prepared as a raw material, and can be stimulated and be formed mature and stable collagenous tissue, and recurrence rate is reduced.
Detailed description of the invention
Fig. 1 is I-type collagen expression after rat abdominal hernia model herniorrhaphy.
Fig. 2 is type III collagen expression after rat abdominal hernia model herniorrhaphy.
Fig. 3 is I/III collagen type expression contents ratio after rat abdominal hernia model herniorrhaphy.
Specific embodiment
The specific embodiment of form by the following examples makees further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following embodiment.
Wherein, agents useful for same of the present invention is common agents, can be purchased from conventional reagent production and sales company.
1 one kind of embodiment prevents adhesion anti-infectious hernia photo fix
Including the composite membrane that polypropylene substrate and polypropylene substrate peripheral hardware are set, the composite membrane is mainly by degradable macromolecule
50 parts of material, 45 parts of chitosan and 5 parts of calcium polyphosphate fiber are made, and the degradable high polymer material is relative molecular weight 250,000
L-lactic acid.
Preparation method:
I, the degradable high polymer material of corresponding amount is taken to be dissolved in the acetone that w/v is 1:5, under confined conditions
Stirring makes it completely dissolved, and ultrasonic deaeration obtains mixed liquor A；
II, to take the chitosan of corresponding amount and calcium polyphosphate fiber to be dissolved in the concentration that w/v is 1:5 be 0.5mol/
In the acetic acid of L, stirring is made it completely dissolved under confined conditions, and ultrasonic deaeration obtains mixed liquid B；
III, mixed liquor A obtained by step I is mixed with mixed liquid B obtained by step II, stirs evenly, is poured into and is covered with poly- third
In the mold of alkenyl piece, evaporation solvent at film to get.
2 one kind of embodiment prevents adhesion anti-infectious hernia photo fix
Including the composite membrane that polypropylene substrate and polypropylene substrate peripheral hardware are set, the composite membrane is mainly by degradable macromolecule
60 parts of material, 35 parts of chitosan and 5 parts of calcium polyphosphate fiber are made, and the degradable high polymer material is relative molecular weight 350,000
Poly-dl-lactide.
Preparation method reference implementation example 1, wherein the lemon that the organic acid solvent in the step S2 is concentration 0.5mol/L
Acid.
3 one kind of embodiment prevents adhesion anti-infectious hernia photo fix
Including the composite membrane that polypropylene substrate and polypropylene substrate peripheral hardware are set, the composite membrane is mainly by degradable macromolecule
40 parts of material, 50 parts of chitosan and 10 parts of calcium polyphosphate fiber are made, and the degradable high polymer material is relative molecular weight 35
Ten thousand poly-dl-lactide.
Preparation method reference implementation example 1, wherein the vinegar that the organic acid solvent in the step S2 is concentration 0.35mol/L
Acid.
A kind of hernia patch of comparative example 1
Difference from Example 1 is that chitosan is replaced with chitosan oligosaccharide by comparative example 1, remaining parameter and preparation method
Reference implementation example 1.
A kind of hernia patch of comparative example 2
Difference from Example 1 is that comparative example 2 removes calcium polyphosphate fiber, increases degradable high polymer material extremely
55 parts, remaining parameter and preparation method reference implementation example 1.
A kind of hernia patch of comparative example 3
Difference from Example 1 is that l-lactic acid is replaced with polycaprolactone by comparative example 3, remaining parameter and system
Preparation Method reference implementation example 1.
A kind of hernia patch of comparative example 4
Polypropylene and expanded polytetrafluoroethylsealing are prepared with reference to the specific embodiment of Chinese patent application CN102058442A
Compound hernia patch.
1 hernia patch of test example repairs the test of rat abdominal-wall defect
1. test material: hernia patch prepared by embodiment 1 and comparative example 1~4.
2. subjects: healthy adult male SD rat, 150~200g of weight, by Zhongshan University SPF grades of Animal Lab.
It provides.
3. test method:
Rat is divided into 5 groups using random digits table: example 1 group and 1~4 group of comparative example.It is pressed with 4% chloraldurate
0.1ml/10g dosage intraperitoneal injection of anesthesia takes abdomen median incision, cuts subcutaneous tissue, blunt separation subcutaneous tissue and deep layer
Fascia appears muscle layer, cuts off two sides abdominal wall muscle and peritonaeum, forms the rectangle defect of size about 2.5 × 2cm, stomach wall is made
Hernia model.Normal saline flushing abdominal cavity, after blotting net residual liquid, each group rat uses the phase of onesize (3cm × 2cm) respectively
Hernia patch is answered to carry out ventral hernia repair art, with 5-0 wire discontinuous sewing hernia patch in abdominal-wall defect muscle edge, 4-0
Line interrupted suture subcutaneous tissue and skin, Sterilized application cover notch, tail label, sub-cage rearing.
General activity, the meal situation of postoperative close observation rat, and whether there is or not infections of incisional wound and hernia patch set-down location skin
The formation such as lower hemotoncus, hernia, fistula.Put to death animal in batches respectively at postoperative 4,6,8 weeks, extraction operative region tissue is made pathology and cuts
Piece observes the postoperative hernia patch surrounding tissue growing state of each group, detects I type, type III collagen with detected by Western blot
Expression and its expression ratio.
4. test result:
During test, no SD rats death situation smoothly completes operation.Clinical follow, all test rats are general
Movable as usual, feed is good, and defecation situation is normal, does not occur situations such as hernia, fistula at notch and hernia patch placement region, week
Tissue growth situation is enclosed referring to table 1, and I type, type III collagen expression and its expression ratio are referring to Fig. 1~3.
1 hernia patch surrounding tissue growing state of table
Seen from table 1, the embodiment of the present invention 1 prepare hernia patch can extraordinary reparations rat abdominal-wall defect, perform the operation
Hernia patch surrounding tissue soft-touch afterwards, foreign body sensation is less, and without adhesion phenomenon, tissue growth is all right, a large amount of fibers
Tissue encapsulation sticking patch, thickness is thicker, and sticking patch grid is invisible, does not have the case where infecting, and 1~4 using effect of comparative example
It is poor.
In addition, collagen is the important extracellular matrix for maintaining connective tissue mechanical stability, is placed in sticking patch
In repair process, collagen is unbalance or generates the factors of abnormal collagenous fibres all will lead to the weakening of connective tissue, thinning,
Eventually lead to hernia recurrence.The collagen overwhelming majority as extracellular matrix main ingredient is by I and type III collagen structure
At, it is extremely rich in type III collagen in inmature granulation and scar, and type i collagen content is considerably less, as fibrosis continues,
Initially dominance type III collagen fades away, and replaced type i collagen, tissue intensity increases therewith.Studies have shown that I/III
Collagen Type VI ratio is lower, and easier recurrence after herniorrhaphy, I/III collagen ratio should be maintained at 4:1 or so, could maintain connective
The intensity of the stabilization and collagen tension of tissue or scar.
By Fig. 1~3 as it can be seen that the I-type collagen of example 1 group kept higher expression quantity at 4,6,8 weeks after operation,
With high tenacity, the collagenous fibres of formation are thicker, can be in the firm structure of early formation；And type III collagen expression compared with
It is few, it is maintained at 4:1 or so in the 6th, 8 week I/III Collagen Type VI ratio, can stimulate and form mature and stable collagenous tissue, is dropped
Low recurrence rate.And 1~4 group of comparative example of I-type collagen is lower in 4,6,8 weeks vindication amounts, expresses with type III collagen
Almost, I/III Collagen Type VI ratio is in (1~2): 1 or so, it be easy to cause recurrence.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (10)
- The anti-infectious hernia photo fix 1. one kind prevents adhesion, which is characterized in that the hernia photo fix is mainly by polypropylene substrate and composite membrane It is made, the composite membrane is mainly made of following raw material and its parts by weight: 30~60 parts of degradable high polymer material, chitosan 20~50 parts and 2~10 parts of calcium polyphosphate fiber.
- 2. hernia photo fix according to claim 1, which is characterized in that the composite membrane is mainly by following raw material and its parts by weight It is made: 50~60 parts of degradable high polymer material, 35~50 parts of chitosan and 5~10 parts of calcium polyphosphate fiber.
- 3. hernia photo fix according to claim 2, which is characterized in that the composite membrane is mainly by following raw material and its parts by weight It is made: 50 parts of degradable high polymer material, 45 parts of chitosan and 5 parts of calcium polyphosphate fiber.
- 4. any hernia photo fix according to claim 1~3, which is characterized in that the degradable high polymer material is selected from left-handed One or both of polylactic acid or poly-dl-lactide.
- 5. hernia photo fix according to claim 4, which is characterized in that the relative molecular weight of the degradable high polymer material is 10 Ten thousand~400,000.
- 6. any hernia photo fix according to claim 1~3, which is characterized in that the preparation method of the hernia photo fix, including it is following Step:S1, take the degradable high polymer material of corresponding amount be dissolved in w/v be 1:(5~20) organic solvent in, it is closed Under the conditions of stir and make it completely dissolved, ultrasonic deaeration obtains mixed liquor A；S2, take the chitosan of corresponding amount and calcium polyphosphate fiber be dissolved in w/v be 1:(5~20) organic acid solvent In, stirring makes it completely dissolved under confined conditions, and ultrasonic deaeration obtains mixed liquid B；S3, mixed liquor A obtained by step S1 is mixed with mixed liquid B obtained by step S2, stirs evenly, is poured into and is covered with polypropylene-base In the mold of piece, evaporation solvent at film to get.
- 7. hernia photo fix according to claim 6, which is characterized in that the organic solvent in the step S1 is selected from acetone, acetic acid One of ethyl ester, chloroform and tetrahydrofuran.
- 8. hernia photo fix according to claim 6, which is characterized in that organic acid solvent in the step S2 be concentration 0.35~ The acetic acid of 0.7mol/L or the citric acid of 0.4~0.6mol/L.
- 9. the preparation method of any hernia photo fix according to claim 1~8, which comprises the following steps:I, take the degradable high polymer material of corresponding amount be dissolved in w/v be 1:(5~20) organic solvent in, it is described Organic solvent is selected from one of acetone, ethyl acetate, chloroform and tetrahydrofuran, and stirring keeps it completely molten under confined conditions Solution, ultrasonic deaeration obtain mixed liquor A；II, take the chitosan of corresponding amount and calcium polyphosphate fiber be dissolved in w/v be 1:(5~20) organic acid solvent In, the organic acid solvent be 0.35~0.7mol/L of concentration acetic acid or 0.4~0.6mol/L citric acid, under confined conditions Stirring makes it completely dissolved, and ultrasonic deaeration obtains mixed liquid B；III, mixed liquor A obtained by step I is mixed with mixed liquid B obtained by step II, stirs evenly, is poured into and is covered with polypropylene-base In the mold of piece, evaporation solvent at film to get.
- 10. preparation method described in any hernia photo fix or claim 9 treats groin in preparation according to claim 1~8 The directly application in the bio-medical material of the oblique hernia of Hernia, femoral hernia, umbilical hernia, hernia of white line or incisional hernia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811527525.5A CN109453429A (en) | 2018-12-13 | 2018-12-13 | One kind prevents adhesion anti-infectious hernia photo fix and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811527525.5A CN109453429A (en) | 2018-12-13 | 2018-12-13 | One kind prevents adhesion anti-infectious hernia photo fix and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109453429A true CN109453429A (en) | 2019-03-12 |
Family
ID=65613252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811527525.5A Withdrawn CN109453429A (en) | 2018-12-13 | 2018-12-13 | One kind prevents adhesion anti-infectious hernia photo fix and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109453429A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714707A (en) * | 2019-03-19 | 2020-09-29 | 陕西佰傲再生医学有限公司 | Anti-infection anti-adhesion composite hernia repair patch and preparation method thereof |
| EA039154B1 (en) * | 2019-05-29 | 2021-12-10 | Сархан Абульфаз оглы Тагиев | Method for manufacturing mesh prosthesis for hernioplasty with prolonged antibacterial properties |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101301491A (en) * | 2008-07-07 | 2008-11-12 | 四川大学 | Polyaldehyde-based sodium alginate cross-linked calcium polyphosphate/chitosan composite scaffold and its preparation and application |
| US20130143976A1 (en) * | 2010-07-01 | 2013-06-06 | Sofradim Production | Medical device with predefined activated cellular integration |
| CN106659819A (en) * | 2014-07-24 | 2017-05-10 | 维尔纳·恩斯特·路德维格·格奥尔格·米勒 | A printable morphogenetic stage-specific chitosan‐polycalcium polyphosphate scaffold for bone repair |
| CN106693058A (en) * | 2015-07-14 | 2017-05-24 | 武汉蓝普医品有限公司 | Production method of partially absorbable fibrous membrane hernia repair patch |
| RU181203U1 (en) * | 2018-04-23 | 2018-07-05 | Общество с ограниченной ответственностью "Специальная и медицинская техника" (ООО "Спецмедтехника") | HEMOSTATIC PLASTER |
-
2018
- 2018-12-13 CN CN201811527525.5A patent/CN109453429A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101301491A (en) * | 2008-07-07 | 2008-11-12 | 四川大学 | Polyaldehyde-based sodium alginate cross-linked calcium polyphosphate/chitosan composite scaffold and its preparation and application |
| US20130143976A1 (en) * | 2010-07-01 | 2013-06-06 | Sofradim Production | Medical device with predefined activated cellular integration |
| CN106659819A (en) * | 2014-07-24 | 2017-05-10 | 维尔纳·恩斯特·路德维格·格奥尔格·米勒 | A printable morphogenetic stage-specific chitosan‐polycalcium polyphosphate scaffold for bone repair |
| CN106693058A (en) * | 2015-07-14 | 2017-05-24 | 武汉蓝普医品有限公司 | Production method of partially absorbable fibrous membrane hernia repair patch |
| RU181203U1 (en) * | 2018-04-23 | 2018-07-05 | Общество с ограниченной ответственностью "Специальная и медицинская техника" (ООО "Спецмедтехника") | HEMOSTATIC PLASTER |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714707A (en) * | 2019-03-19 | 2020-09-29 | 陕西佰傲再生医学有限公司 | Anti-infection anti-adhesion composite hernia repair patch and preparation method thereof |
| EA039154B1 (en) * | 2019-05-29 | 2021-12-10 | Сархан Абульфаз оглы Тагиев | Method for manufacturing mesh prosthesis for hernioplasty with prolonged antibacterial properties |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Silk structure and degradation | |
| US20010008937A1 (en) | Threads containing hyaluronic acid esters and their use in surgery | |
| CN104353111B (en) | A kind of bioprosthetic material for abdominal-wall defect and preparation method thereof | |
| CN108348660B (en) | Method for manufacturing high-performance suture coated with hyaluronate and high-performance suture thereof | |
| Jensen et al. | Biomimetic and synthetic esophageal tissue engineering | |
| CN108261565B (en) | Hernia patch, preparation method and application thereof in hernia repair | |
| CN109453429A (en) | One kind prevents adhesion anti-infectious hernia photo fix and preparation method thereof | |
| CN109893680A (en) | A kind of repairing fiber film repairs socket pipe and its preparation method and application | |
| CN103980386A (en) | Acylated chitin fiber and preparation method thereof, and application of acylated chitin fiber in preparation of surgical suture | |
| CN112430292A (en) | Antibacterial and degradable sodium alginate-based polycaprolactone copolymer and application thereof | |
| EP1938774B2 (en) | Medical artificial nerve graft containing silk fibroin and its preparation method | |
| KR102776357B1 (en) | A novel resorbing biodegradable medical and cosmetic composition | |
| CN104998299A (en) | Decellularized anti-calcification heart patch and preparation method thereof | |
| CN105251061A (en) | Biodegradable postoperative anti-adhesion composite membrane and preparation method thereof | |
| Stoop et al. | Advanced age alone does not suppress anastomotic healing in the intestine | |
| CN104473706A (en) | Biodegradable composite type tubular urethral stent and preparation method | |
| CN109234843B (en) | Polylactide and polydioxanone composite fiber, its preparation method, use and surgical suture | |
| CN110279902A (en) | A kind of neurosurgery post-operation anti-adhesion film and preparation method thereof | |
| CN103100116A (en) | Tissue adhesion prevention material, medicine-carrying material and preparation method thereof | |
| CN111714707A (en) | Anti-infection anti-adhesion composite hernia repair patch and preparation method thereof | |
| CN105381501A (en) | Degradable biological composite haemostasis membrane and preparation method thereof | |
| CN109865159A (en) | A kind of silk network and its preparation method and application | |
| CN107998460A (en) | A kind of Antiadhesive film with antibacterial anti hemorrhagic and preparation method thereof | |
| CN116392646A (en) | Barrier membrane for guiding bone regeneration | |
| CN111068124B (en) | Polypropylene/tea polyphenol patch for intraperitoneal repair and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190312 |