CN109444075A - A method of glucose concentration is monitored based on OCT technology - Google Patents
A method of glucose concentration is monitored based on OCT technology Download PDFInfo
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- CN109444075A CN109444075A CN201811293204.3A CN201811293204A CN109444075A CN 109444075 A CN109444075 A CN 109444075A CN 201811293204 A CN201811293204 A CN 201811293204A CN 109444075 A CN109444075 A CN 109444075A
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 50
- 239000008103 glucose Substances 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000005516 engineering process Methods 0.000 title claims abstract description 17
- 238000005259 measurement Methods 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000003325 tomography Methods 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 230000009466 transformation Effects 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 abstract description 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 238000012014 optical coherence tomography Methods 0.000 description 20
- 238000001514 detection method Methods 0.000 description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000002479 acid--base titration Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
本发明公开了一种基于OCT技术监测葡萄糖溶液浓度的方法，主要利用OCT测出葡萄糖折射率，而葡萄糖浓度和液体折射率在恒温、恒压下呈线性关系，利用该线性关系，可由折射率变化测试浓度的变化。具体过程是：首先测量多组不同浓度的葡萄糖溶液的折射率，拟合出葡萄糖与折射率的关系曲线；固定管腔先充满空气和再充满未知浓度葡萄糖溶液，用OCT分别测定获得光程d1和d2，根据公式n=d2/d1计算出未知葡萄糖液体折射率；将折射率代入先前获得的浓度与折射率关系表达式即求得未知葡萄糖溶液浓度。本方法操作简便、测量精准，能快速对葡萄糖等液体的浓度进行测量，在液体浓度测量领域充分发挥其作用。
The invention discloses a method for monitoring the concentration of glucose solution based on OCT technology, mainly using OCT to measure the refractive index of glucose, and the glucose concentration and the refractive index of the liquid have a linear relationship under constant temperature and constant pressure. Changes in test concentrations. The specific process is: first measure the refractive index of several groups of glucose solutions with different concentrations, and fit the relationship curve between glucose and refractive index; the fixed lumen is filled with air first and then filled with unknown concentration of glucose solution, and the optical path d1 is obtained by OCT measurement respectively. and d2, the unknown glucose liquid refractive index is calculated according to the formula n=d2/d1; the unknown glucose solution concentration is obtained by substituting the refractive index into the previously obtained expression of the relationship between the concentration and the refractive index. The method is easy to operate, accurate in measurement, can quickly measure the concentration of liquids such as glucose, and fully exert its function in the field of liquid concentration measurement.
Description
Technical field
The present invention relates to optical measurement and solution concentration fields of measurement, it is related to realizing glucose based on OCT technology with a kind of
The measurement of solution concentration.
Background technique
Solution concentration measurement is one basic working procedure of industrial production, has in industrial circles such as food, chemical industry, pharmacy and answers extensively
With, but structure is complicated for related device, and it is expensive, and without alternative solution, measuring principle mainly measures corresponding solution refraction
Rate variation.
The measurement method of solution concentration includes measuring instrument, evaporation, and the method that acid or alkali can use acid-base titration is sunk
Shallow lake method, densimetry etc.；But all cannot quick to solution concentration, real-time, non-contact measurement, automation, intelligence degree it is poor,
The present invention is based on optical coherence tomography (OCT) technology and appropriate reconstructions, it will thus provide a kind of competitive measurement is new
Method.
Optical coherence tomography (OCT) technology measures refractive index, as long as sample to be tested is directly placed scanning model
It encloses, itself having is a kind of quick, non-contact detection refractive index characteristic new technology, but centre also needs manual intervention, reflects
Rate measurement is without corresponding software kit, and far from full real time implementation, automation, intelligence, the present invention utilizes OCT prior art, and sets
Peculiar luminal structure (see attached drawing 3) and matching measuring principle are counted, thus promotes the technology to real time implementation, automation, intelligence
Level can be changed.
Summary of the invention
The present invention provides a kind of method for measuring glucose concentration, and this method had both been able to achieve to glucose concentration
Quickly, in real time, non-contact measurement, and be able to achieve full-automatic, intelligentized measurement.
To realize above-mentioned technical characterstic, this method is based on OCT technology, comprising the following steps:
A, using the refractive index of Abbe instrument measurement different glucose solution, and it is fitted to curve, for use；
B, vacant lumen is scanned using OCT and obtains unloaded tomography digital picture, obtain lumen zero load thickness d 1；
C, it will be full of glucose solution to be measured in lumen, and be scanned the tomography digital picture for obtaining sample to sample；
D, when processing image obtains lumen and is full of glucose solution to be measured, the thickness d 2 on image, according to principle formula n=d2/d1
Obtain the refractive index of glucose solution to be measured；
E, according to the relation curve of the concentration and refractive index fitted, refractive index is substituted into, it is molten further to obtain glucose to be measured
Liquid concentration.
Particularly, the lumen is built by two panels transparent glass sheet, is square column pipe, but the available lumen of this method
Structure is not limited only to described herein this.
When lumen is vacant, OCT is realized to the inside pipe wall Tomography；Further, grape to be measured will be full of in pipe
Sugar juice sample, OCT is to the same Tomography of the inside pipe wall.
Particularly, it in conjunction with Hough transform (Hough Transformation) algorithm, is realized automatically by MATLAB software
The feature extraction of OCT Drawing Object identification and Drawing Object, but the available method and software for extracting boundary of this method, not only limit
In described herein this.
Further, identification obtains the distance feature between straight line, and the refractive index of glucose solution to be measured is calculated according to principle.
Further, using the glucose concentration of Abbe instrument measurement and the matched curve of refractive index, it is related to linearly returning
Analysis and other approximating methods are returned to provide concentration value by concentration and refractive index corresponding relationship.
The beneficial effects of the present invention are: providing a kind of method for testing glucose concentration value, this method is expected to realize
Quick to glucose concentration, real-time, non-contact measurement, while it being able to achieve full-automatic, intelligentized measurement again, it can
Production detection for a variety of solution of industry.
Detailed description of the invention
Fig. 1 is glucose concentration provided by the present invention and index of refraction relationship figure.
Fig. 2 is method and step figure provided by the present invention.
Fig. 3 is measuring principle device figure provided by the present invention.
Fig. 4 is one group of embodiment OCT dynamic scan figure provided by the present invention.
Fig. 5 is processing result figure provided by the present invention, parameter extraction figure.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, with reference to the accompanying drawing and specifically real
Applying example, the invention will be further described.
The present invention provides a kind of method for testing glucose concentration, and this method is based on OCT technology, including following step
It is rapid:
A, using the refractive index of Abbe instrument measurement different glucose solution, and it is fitted to curve, for use；
B, vacant lumen is scanned using OCT and obtains unloaded tomography digital picture, obtain lumen zero load thickness d 1；
C, it will be full of glucose solution to be measured in lumen, and be scanned the tomography digital picture for obtaining sample to sample；
D, when processing image obtains lumen and is full of glucose solution to be measured, the thickness d 2 on image, according to principle formula n=d2/d1
Obtain the refractive index of glucose solution to be measured；
E, according to the relation curve of the concentration and refractive index fitted, refractive index is substituted into, it is molten further to obtain glucose to be measured
Liquid concentration.
As shown in Figure 1, measuring glucose concentration and index of refraction relationship figure, this hair for Abbe instrument provided by the present invention
The bright refractive index obtained after obtaining faultage image using OCT measurement and being handled can be matched according to curve to obtain
Glucose concentration to be measured.
As shown in figure 3, being measuring principle device figure provided by the present invention, lumen is built by two panels transparent glass sheet,
For square column pipe；Unloaded tomography digital picture is obtained firstly, being scanned using OCT to vacant lumen, algorithm process obtains
Take lumen zero load thickness d 1；It will be full of glucose solution to be measured in lumen, and acquisition solution is scanned to solution to be measured and is broken
The processing of layer digital image algorithm obtains liquid-filled lumens thickness d 2；The refraction of glucose solution is finally obtained according to formula n=d2/d1
Rate.
As shown in figure 4, being one group of embodiment OCT dynamic scan figure provided by the present invention, sample is respectively various concentration
Glucose solution, change glucose concentration during obtaining sample drawing.
As shown in figure 5, realizing OCT automatically by software in conjunction with Hough transform (Hough Transformation) algorithm
The feature extraction of Drawing Object identification and Drawing Object, identification obtain the distance feature between straight line, are calculated according to principle formula molten
The refractive index of liquid.
Fig. 5 processing gained refractive index results are substituted into the provided concentration of Fig. 1-index of refraction relationship curve to get to be measured molten out
Liquid concentration value.
The application example utilizes OCT measurement result: n=1.3432, concentration 7%；N=1.3495, concentration 11%；n=
1.355, concentration 15%；N=1.365, concentration 22%.
Claims (7)
1. the invention discloses it is a kind of using OCT technology measurement glucose concentration method, feature the following steps are included:
A, using the refractive index of Abbe instrument measurement different glucose solution, and it is fitted to curve, for use；
B, vacant lumen is scanned using OCT and obtains unloaded tomography digital picture, obtain lumen zero load thickness d 1；
C, it will be full of glucose solution to be measured in lumen, and be scanned the tomography digital picture for obtaining sample to sample；
D, processing image obtains the thickness d 2 on image of the lumen full of glucose solution to be measured when, is obtained according to formula n=d2/d1
To the refractive index of glucose solution to be measured；
E, according to the relation curve of the concentration and refractive index fitted, refractive index is substituted into, it is molten further to obtain glucose to be measured
Liquid concentration.
2. a kind of method using OCT technology measurement concentration of glucose according to claim 1, it is characterised in that: described
Glucose concentration-refractive index curve chart is obtaining faultage image using OCT measurement and is being located for the present invention in step a
After managing obtained refractive index, it can be matched according to curve to obtain glucose concentration to be measured.
3. a kind of method using OCT technology measurement concentration of glucose according to claim 1, it is characterised in that: described
Lumen is built by two panels transparent glass sheet, is square column pipe, but the available luminal structure of this method is not limited only to retouch herein
That states is this.
4. a kind of method using OCT technology measurement concentration of glucose according to claim 1, it is characterised in that: described
Step b, principle involved by c, d are as follows: obtain a certain tomography OCT image when lumen " vacant " (being full of air) first, and by image
Measure " air light path " d1；Full of after testing liquid in lumen, corresponding " liquid light path " d2 of same tomography is obtained；Principle is public
Formula: liquid refractivity=liquid light path ÷ air light path, i.e. n=d2/d1.
5. a kind of method using OCT technology measurement concentration of glucose according to claim 1, it is characterised in that: described
Step d combination Hough transform (Hough Transformation) algorithm, realized automatically by software OCT Drawing Object identification and
The feature extraction of Drawing Object.
6. a kind of method using OCT technology measurement concentration of glucose according to claim 4, it is characterised in that: identification
The distance feature between straight line is obtained, the refractive index of liquid is calculated according to principle.
7. a kind of method using OCT technology measurement concentration of glucose according to claim 1, it is characterised in that: utilize
The relation curve of step a glucose concentration being fitted and refractive index is provided by concentration and refractive index corresponding relationship
Concentration value.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811293204.3A CN109444075A (en) | 2018-11-01 | 2018-11-01 | A method of glucose concentration is monitored based on OCT technology |
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| CN201811293204.3A CN109444075A (en) | 2018-11-01 | 2018-11-01 | A method of glucose concentration is monitored based on OCT technology |
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| US20070219437A1 (en) * | 2006-03-17 | 2007-09-20 | Glucolight Corporation | System and method for creating a stable optical interface |
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|---|---|---|---|---|
| US8078244B2 (en) * | 2005-12-14 | 2011-12-13 | Newton Photonics, Inc. | Interferometric method and instrument for measurement and monitoring blood glucose through measurement of tissue refractive index |
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| ESENALIEV, RO: "Noninvasive monitoring of glucose concentration with optical coherence tomography", 《OPTICS LETTERS》 * |
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Application publication date: 20190308 |