Summary of the invention
It is an object of the invention to overcome above-mentioned the deficiencies in the prior art, a kind of Cut Flower Chrysanthemum Morifolium is provided adopts rear bottle and insert fresh-keeping work
Skill improves consumer's fresh flower quality, provides technical guarantee for Cut Flower Export quality.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of Cut Flower Chrysanthemum Morifolium adopts rear bottle and inserts antistaling process, comprising the following steps:
A, the preparation of Cut Flower Chrysanthemum Morifolium: the close spray of just splitting of early morning acquisition development, corolla 2-3cm, the long 60-70cm of spray, after adopting
It is sent to laboratory at once, retains top 10-12 piece leaf, shearing stays 4 leaves to 50cm spray in water;The step can prevent
Excessive water evaporation, guarantees the form consistency of experiment chrysanthemum.
B, the pretreatment of Cut Flower Chrysanthemum Morifolium: dipping spray base portion 10s with treatment fluid, is inserted into distilled water after drying, treatment fluid is
At least one of NAA of IBA-K, 40mg/L of 40mg/L;The step can be such that effective component in treatment fluid sufficiently cuts with flower material
Mouthful contact, and effectively combined with it, IBA-K, NAA can promote notch cell mitogenetic, on the one hand do sth. in advance healing of wound, prevent from feeling
Dye;The thick embryo of another aspect induced synthesis young root, increases its soaking effect, dries mainly for enabling treatment fluid ingredient to be bonded to
On section.
C, bottle inserts pre-treatment: base portion is tiltedly cut;The step can guarantee that section is smooth, reduce section flower material tissue due to being sheared
Power influences injury, increases its section product, increases the thick embryonal induction face of young root.
D, bottle inserts processing: bottle slotting ring border is interior, and air average relative humidity is 65%, using indoor daylight lamp as light source,
Intensity of illumination is 1500lux, and room temperature is 20 ± 2 DEG C, and spray inserts in the mineral water bottle of 500ml cleaning, each mineral water
There is 500ml antistaling agent in bottle, each mineral water bottle inserts one, the potassium sorbate+2g/L for the sucrose+100mg/L that antistaling agent is 2%
CaCl2The citric acid of+100mg/L.Low concentration sucrose, which is that flower material bottle is slotting, provides energy;Potassium sorbate is preservative, prevents sugarcane
Sugar, which is added, breeds bacterium, influences to spend material section;The normal growth for being incorporated as spending material of calcium chloride provides calcium ion, believes its cell
Number conduction can be normally carried out;Citric acid is conducive to spend to guarantee that chrysanthemum cell maintains under a relatively weak pH environment
Material growth.
Any one of Cut Flower Chrysanthemum Morifolium in feverfew, bull chrysanthemum, yellow chrysanthemum flower.
The beneficial effects of the present invention are: antistaling agent is to plant energy supply, flower, leaf plumpness are preferable after bottle is inserted, stem
The antibacterial effect of stalk is also preferable;Consumer's fresh flower quality is improved, provides technical guarantee for Cut Flower Export quality.
Specific embodiment
Present invention will be further described below with reference to the accompanying drawings and specific embodiments:
Test chrysanthemum used: fresh feverfew, bull chrysanthemum, yellow chrysanthemum flower.
Reagent: conventional chemical reagent is purchased from the raw work in Shanghai;Total protein is quantitative, plant soluble sugar, malonaldehyde (MDA) are tested
Box is purchased from Nanjing and builds up.
Key instrument: centrifuge, Nanodrop 2000, vernier caliper, day equality.
A kind of Cut Flower Chrysanthemum Morifolium adopts rear bottle and inserts antistaling process, comprising the following steps:
A, the preparation of Cut Flower Chrysanthemum Morifolium: the close spray of just splitting of early morning acquisition development, corolla 2-3cm, the long 60-70cm of spray, after adopting
It is sent to laboratory at once, retains top 10-12 piece leaf, shearing stays 4 leaves to 50cm spray in water;
B, the pretreatment of Cut Flower Chrysanthemum Morifolium: dipping spray base portion 10s with treatment fluid, is inserted into distilled water after drying, treatment fluid is
At least one of NAA of IBA-K, 40mg/L of 40mg/L;
C, bottle inserts pre-treatment: base portion is tiltedly cut;
D, bottle inserts processing: bottle slotting ring border is interior, and air average relative humidity is 65%, using indoor daylight lamp as light source,
Intensity of illumination is 1500lux, and room temperature is 20 ± 2 DEG C, and spray inserts in the mineral water bottle of 500ml cleaning, each mineral water
There is 500ml antistaling agent in bottle, each mineral water bottle inserts one, the potassium sorbate+2g/L for the sucrose+100mg/L that antistaling agent is 2%
CaCl2The citric acid of+100mg/L.
Any one of Cut Flower Chrysanthemum Morifolium in feverfew, bull chrysanthemum, yellow chrysanthemum flower.
As can be drawn from Figure 1, for antistaling agent to plant energy supply, it is preferable that bottle inserts performance in antibiotic property.Bottle is inserted 20 days, flower
Piece, leaf plumpness it is preferable, the antibacterial effect of stalk is also preferable, obvious floccule does not occur.Tap water is compared with distilled water
There is the more brown precipitate of stalk, this has a certain concentration chlorine residue related with its early stage.
1. spending the measurement of diameter: with the diameter of 3 different directions of vernier caliper measurement flower, taking its average value.It is inserted from cut-flower
It is risen on the day of entering solution, the flower diameter of measurement in every two days, and first day difference is flower diameter variation.
2. the measurement of cut-flower fresh weight and water balance value: start on the day of Fresh Cutting flower insertion antistaling agent, the spray that weighs with scale,
The sum of antistaling agent and weight of drink bottle are m1, then it is m2 that cut-flower taking-up, which is measured the sum of antistaling agent and weight of drink bottle,.It surveys afterwards for 24 hours
Measuring the sum of spray, antistaling agent and weight of drink bottle is m1 ', and its antistaling agent and weight of drink bottle m2 ';Spray fluid loss is m2 '-
M2, spray water absorption are m1 '-m1, and water balance value is water absorption-fluid loss.
3. the measurement of soluble protein, soluble sugar: weighing Chrysanthemum Petal 0.05-0.1g, (fresh plant petal shreds mixed
It is even), by weight (g): the distilled water of volume (ml)=1:9 ratio 9 times of volumes of addition, ice-water bath condition mechanical homogenisation, 2500
Rev/min, it is centrifuged 10 minutes, takes supernatant distilled water to be homogenized by 1:9 dilution proportion at 1% concentration, built up and purchased using Nanjing
The protein quantification testing cassete bought, the kit use Coomassie Brilliant Blue, measure protein concentration;Take homogenate, boiling water bath
10min, after cooling, 4000 revs/min, room temperature is centrifuged 10min, 20 times of supernatant distilled water dilutions is taken, with plant soluble sugar
Detection kit (using anthrone colorimetry) measurement.
4. the measurement of mda content: building up malonaldehyde (MDA) testing cassete of purchase using Nanjing, which uses
TBA method.
As can be drawn from Figure 2, downward trend after first rising is presented in MDA changes of contents, and bottle inserts early period due to by nutrition supplying
To the influence of interruption, MDA content is risen rapidly, and the phenomenon of the more MDA high of nutritional ingredient is added in the slotting antistaling agent of bottle, but with
Downward trend is presented in the increase of bottled sustaining time, and later period MDA change rate is stablized, and there was no significant difference.
As can be drawn from Figure 3, soluble protein fluctuation is larger, but basic presentation increase trend.
As can be drawn from Figure 4, antistaling agent is inserted using bottle, the influence to flower diameter is not too greatly at first Hua Jingcong increases to
Last wilting reduction.Experiment shows that distilled water group flower diameter increases very fast, flowering fast speed.But antistaling agent group and distillation
Water group flower diameter change rate is smaller, illustrates that the flowering time is longer, and cut-flower isolated growth is stablized.
As can be drawn from Figure 5, measurement early period fluctuation is larger, and it is smaller that later period bottle inserts fluctuation, distilled water group and antistaling agent group compared with
For stabilization, deviation is also smaller, in conjunction with Fig. 1 we it can also be seen that bottle insert the full color of later period cut-flower it is still more bright-coloured.
As can be drawn from Figure 6, the variation of soluble sugar has certain correlation with soluble protein variation, and basic present increases
Trend.
As can be drawn from Figure 7, the fresh weight of two control group Cut Flower Chrysanthemum Morifoliums influences smaller, and antistaling agent group slightly increases on fresh weight
Add, this has certain relationship with sucrose is added to.
Test is inserted by the primary dcreening operation of antistaling agent and long-term bottle, low dosage addition sucrose has centainly the late growth of cut-flower
Facilitation, but the consequences such as solution bacteria breed can be brought, so additive amount cannot be too big, and it was found that in selection anti-corrosion
In the antibacterial means such as agent, antibiotic, bacteriostatic agent, the effect of preservative is better than antibiotic, and addition antibiotic will appear stalk hair
Black phenomenon influences cut-flower late growth especially moisture supply.In addition, the bacteriostatic agent using metal ion will affect blade
Color and stalk color.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.