CN109395625B - Reaction liquid stirring method and mechanism and in-vitro detection equipment - Google Patents
Reaction liquid stirring method and mechanism and in-vitro detection equipment Download PDFInfo
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- CN109395625B CN109395625B CN201710703172.9A CN201710703172A CN109395625B CN 109395625 B CN109395625 B CN 109395625B CN 201710703172 A CN201710703172 A CN 201710703172A CN 109395625 B CN109395625 B CN 109395625B
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Abstract
The invention discloses a stirring method and a stirring mechanism and in-vitro detection equipment. Specifically, the invention provides a stirring rod for stirring reaction liquid; acquiring detection methodology information of the reaction solution; obtaining stirring parameters according to the detection methodology information, wherein the stirring parameters at least comprise the motion trail information of the stirring rod; and controlling the stirring rod to stir the reaction liquid according to the stirring parameters. The invention correspondingly stirs the reaction liquid according to the detection methodology information of the reaction liquid, has the advantages of pertinence, strong mixing capability and the like, and improves the test speed and the test accuracy.
Description
Technical Field
The invention relates to the field of medical detection, in particular to a method and a mechanism for stirring reaction liquid and in-vitro detection equipment.
Background
In the field of automatic detection of medical instruments, obtaining uniform reaction liquid and stable reaction rate has important significance for the performance of a detection system, so that a mechanism for assisting uniform mixing of the reaction liquid is necessary. The key point of the uniform mixing is to improve the diffusion speed and the relative contact area of the reagent in the sample, so that the reagent and the sample form uniform reaction liquid in the shortest time, and a stable reaction rate is created for the detection process.
The existing mixing modes mainly comprise two types:
the first mode is that the clamping jaw is vibrated at high speed to form annular eddy currents in the reaction cup, the uniform mixing mode has good effect on the reaction cup with a circular cross section, but has poor effect on the reaction cup with a rectangular cross section adopted by a double-magnetic-circuit magnetic bead method, and bubbles are easily formed in the high-speed vibration process to interfere with the test;
the second mode is to mix the reaction liquid by absorbing and spitting and stirring the reaction liquid through the needle tube, and the mixing mode is to realize mixing by generating vertical movement of the liquid through the absorbing and spitting functions of the needle tube and driving the horizontal movement of the liquid through the stirring function.
Disclosure of Invention
The invention provides a reaction liquid stirring method and mechanism and in-vitro detection equipment.
According to a first aspect, the present invention provides a method for stirring a reaction solution, comprising:
providing a stirring rod for stirring the reaction liquid;
acquiring detection methodology information of the reaction solution;
obtaining stirring parameters according to the detection methodology information, wherein the stirring parameters at least comprise the motion trail information of the stirring rod;
and controlling the stirring rod to stir the reaction liquid according to the stirring parameters.
The present invention also provides another method for stirring a reaction solution, comprising:
providing a stirring rod for stirring the reaction liquid;
acquiring detection methodology information of the reaction solution;
obtaining stirring parameters according to the detection methodology information, wherein the stirring parameters at least comprise the motion trail information of the stirring rod;
judging whether the reagent added into the reaction solution is a trigger reagent;
and when the reagent added into the reaction liquid at present is judged to be the trigger reagent, controlling the stirring rod to stir the reaction liquid according to the stirring parameters.
According to a second aspect, the present invention provides a reaction solution stirring mechanism comprising:
the stirring rod is used for stirring the reaction liquid;
the driving component is used for driving the stirring rod to move;
a processor for performing the blending method of any of the above embodiments.
According to a third aspect, the invention provides an in vitro detection device comprising the stirring mechanism described above.
According to the stirring method and mechanism and the in-vitro detection device, the reaction liquid is stirred correspondingly according to the detection methodology information of the reaction liquid, the advantages of pertinence, strong mixing capability and the like are achieved, and the test speed and the test accuracy are improved.
Drawings
FIG. 1 is a flow chart of a stirring method of an embodiment;
FIGS. 2(a) and (b) are schematic diagrams respectively illustrating the motion traces of the stirring rod disclosed in the stirring method according to an embodiment;
FIG. 3 is a schematic structural view of a container having a rectangular cross section provided in one embodiment;
FIG. 4 is a schematic structural diagram of an embodiment of a stirring mechanism;
FIG. 5 is a flow chart of a stirring method according to another embodiment;
FIG. 6 is a flowchart illustrating a method of determining whether a reagent currently added to a reaction solution is a trigger reagent according to an exemplary embodiment;
FIG. 7 is a schematic view showing a travel path of a stirring rod performing one-dimensional motion in a horizontal plane according to an embodiment of a stirring method;
FIG. 8 is a schematic structural view of a stirring mechanism according to another embodiment;
FIG. 9 is a diagram illustrating the stirring parameters of an embodiment.
Detailed Description
The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
As mentioned in the background, the use of jaws or needles for blending has disadvantages; in addition, the inventors also noted that the requirements of different reaction solutions in the process of mixing are also different, for example: the Activated Partial Thromboplastin Time (APTT) adopts ellagic acid as a mixed reagent to form a reaction solution with a sample, and only the sample and the reagent need to be contacted with each other during uniform mixing, so that the diffusion is facilitated; furthermore, the inventors further research has found that when the test items of the reaction solution employ different detection methodologies, the requirements for the reaction solution in the mixing process may be different, for example: the D _ dimer (DD) is measured by latex immunoturbidimetry, which requires the sample and the latex emulsion to be well mixed, but does not introduce air bubbles, etc. Therefore, for supporting multiple detection items and instruments adopting multiple detection methodologies for detection, a single blending mode becomes an important factor for limiting the performance of the instruments.
In view of the above, the inventor has proposed a method for stirring reaction liquids (hereinafter referred to as a stirring method) by using a stirring rod to stir the reaction liquids to achieve the purpose of uniform mixing, in consideration of not only the disadvantage of uniform mixing by using a single clamping jaw or a needle tube in the prior art, but also the difference in the requirements of uniform mixing of different reaction liquids, typically different reagents to be added, and the difference in the requirements of uniform mixing due to different detection methodologies adopted by the reaction liquids, as described in detail below.
Example one
Referring to fig. 1, an embodiment of a stirring method includes steps S110 to S140.
Step S110: a stirring rod is provided for stirring the reaction solution. In one embodiment, the stirring rod is a round rod made of austenitic stainless steel, and the top end of the stirring rod can be rounded, and the top end of the stirring rod refers to the end of the stirring rod extending into the reaction liquid.
Step S120: and acquiring detection methodology information of the reaction solution. In one embodiment, the detection methodology information may include a first type of detection methodology, which may include immunoturbidimetry, chromogenic substrate method, and the like, and a second type of detection methodology, which may include a dual magnetic bead method, and the like.
Step S130: and obtaining stirring parameters according to the acquired detection methodology information, wherein the stirring parameters at least comprise the motion track information of the stirring rod. In one embodiment, step S130 includes: the puddler motion trajectory information is the two-dimensional motion in the puddler execution vertical plane. In a particular embodiment, the stirring rod performs a two-dimensional motion in a vertical plane, comprising: the stirring rod repeatedly executes a movement on a closed trajectory which is composed of several curved paths and/or line segment paths in the same vertical plane. As mentioned above, the blending parameter at least includes the trace information of the blending rod movement, and in an embodiment, the blending parameter further includes at least one of the initial position of the top end of the blending rod, the speed of the blending rod movement, and the time of the blending rod movement. The stirring parameters can be preset, or can be set by a user through a related interactive interface.
The relationship between the detection methodology information and the stirring parameters in step S130 will be described in detail below.
In an embodiment, in step 130, when the detection methodology information is the first type of detection methodology, the stir bar motion trajectory information is two-dimensional motion of the stir bar in a vertical plane, it should be noted that the initial position of the top end of the stir bar may be any point on the closed trajectory, and the motion direction of the stir bar may be always clockwise or always counterclockwise, or may alternatively be clockwise and counterclockwise; and/or when the detection methodology information is the second type of detection methodology, the motion track information of the stirring rod is that the stirring rod stops at the original position, and stirring is not executed.
As described above, when the detection methodology is the first type of detection methodology, the motion trajectory information of the stirring rod is that the stirring rod performs two-dimensional motion in a vertical plane, for example, the stirring rod repeatedly performs motion on a closed trajectory composed of several curved paths and/or line segment paths in the same vertical plane.
In a specific embodiment, when the detection methodology information is a chromogenic substrate method, the obtained closed trajectory is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths respectively connected with two ends of the horizontal line segment path, and one line segment path connecting one ends of the two vertical line segments, which are not connected with the horizontal line segment. For example, as shown in fig. 2(a) showing an example of a corresponding closed trajectory when the detection methodology information is a chromogenic substrate method, in the actual stirring process, the initial position of the tip of the stirring rod may be any point in the closed trajectory in fig. 2(a), and the description will be given by taking the example that the initial position of the tip of the stirring rod is located at the point a in the figure and moves counterclockwise along the closed trajectory: the stirring rod moves linearly for a certain distance towards the oblique upper direction from the position of the top end of the stirring rod at the point a in the drawing, then moves linearly for a certain distance towards the vertical direction upwards, then moves linearly back towards the horizontal direction to the position right above the point a, and then moves linearly downwards towards the vertical direction to return to the point a, so that the motion of a closed track is completed. It will be appreciated that the initial position of the stirring rod tip may be at any point in fig. 2(a), and the direction of movement may also be clockwise along a closed trajectory.
In one embodiment, when the detection methodology information is immunoturbidimetry, the resulting closed trajectory is formed by two curved paths in the same vertical plane, wherein one curved path is a concave curved path and the other curved path is a convex curved path, one end of the concave curved path is connected to one end of the convex curved path, and the other end of the concave curved path is connected to the other end of the convex curved path. For example, as shown in fig. 2(b) which shows an example of a corresponding closed trajectory when the detection methodology information is immunoturbidimetry, in the actual stirring process, the initial position of the stirring rod tip may be any point in the closed trajectory in fig. 2(b), and the description will be given by taking the example that the initial position of the stirring rod tip is located at the point b in the figure and moves counterclockwise along the closed trajectory as an example: starting from the point b, the motion of a closed track is finished by firstly moving towards the downward direction along the path of the convex curve for a certain distance and then moving towards the upward direction along the path of the concave curve to return to the point b. It will be appreciated that the initial position of the stirring rod tip may be at any point in fig. 2(b), and the direction of movement may also be clockwise along a closed trajectory.
The inventors have studied and found that since a vortex is generated in the vicinity of the tip end of the stirring rod when the stirring rod moves in the reaction liquid, particularly at a high speed, and the distance of movement of the tip end of the stirring rod is relatively long when the two-dimensional movement of the vertical plane is performed, the vortex can be generated over as long a distance as possible when the two-dimensional movement of the vertical plane is performed. The stirring efficiency of FIG. 2(a) is higher than that of FIG. 2(b) in terms of two-dimensional movement trajectories in two vertical planes, while the two-dimensional movement trajectories in two vertical planes of FIG. 2(a) and (b) produce fewer bubbles during the stirring process, so that the movement trajectory of the stirring rod shown in FIG. 2(a) is suitable for stirring a reaction solution using a detection methodology that is insensitive to bubbles but strict with respect to time, such as the chromogenic substrate method described above, while FIG. 2(b) is suitable for stirring a reaction solution using a detection methodology that is more sensitive to bubbles, such as the immunoturbidimetry method described above.
Step S140: and controlling the stirring rod to stir the reaction liquid according to the stirring parameters.
The present invention provides a stirring rod for stirring, and in one embodiment, the stirring method may further include providing a container with a non-circular cross section for containing the reaction solution to match the stirring of the stirring rod, for example, referring to fig. 3, the present invention provides a container with a rectangular cross section. The non-circular cross-section container of the present invention, e.g., a rectangular cross-section container, which incorporates the stir bar of the present invention, has many advantages over a circular cross-section container. For example, for the double magnetic circuit magnetic bead method, it is more suitable to use the reaction vessel of rectangular cross section, the magnetic bead method arranges the electro-magnet around the reaction vessel, produce magnetism through the condition of switching on of control electro-magnet, in order to drive the magnetic bead wobbling, use rectangular cross section reaction vessel can only arrange 2 electro-magnets and just can effectively control the magnetic bead wobbling, the two magnetic circuit magnetic bead method compares optics method and has the leading advantage of anti-jamming, because chyle, jaundice, bilirubin etc. all can lead to the optical property change of reaction solution, influence the testing result, but these materials can not influence the wobbling of magnetic bead, therefore also can not disturb the test of magnetic bead method. For the immunoturbidimetric and chromogenic substrate method, the rectangular reaction container can not focus light beams and influence light paths, the light path arrangement is simpler, and one lens can be omitted compared with a circular reaction container.
The stirring method provided by the embodiment of the invention performs corresponding stirring on the reaction liquid according to the detection methodology information of the reaction liquid, has pertinence and strong uniform mixing capability, and improves the test speed and the test accuracy.
In one embodiment, the in-vitro testing apparatus may include a stirring mechanism for a reaction solution (hereinafter referred to as a stirring mechanism), and referring to fig. 4, the stirring mechanism includes a stirring rod 110, a driving member 120, and a processor 130, which will be described in detail below.
The stirring rod 110 is used for stirring the reaction solution, and in one embodiment, the stirring rod 110 is a round rod made of austenitic stainless steel, and the top end of the round rod can be rounded.
The driving member 120 is used to drive the stirring rod 110 to move. For example, taking a three-dimensional rectangular coordinate system XYZ as an example, the driving unit 120 can drive the stirring rod to perform two-dimensional motion in the horizontal X direction and the vertical Z direction. In one embodiment, the driving member 120 may be implemented using a stepping motor.
The processor 130 is configured to obtain detection methodology information of the reaction solution, and obtain stirring parameters according to the detection methodology information, where the stirring parameters at least include information of a motion trajectory of the stirring rod; and controls the stirring rod 110 to stir the reaction solution by controlling the driving part 120 according to the stirring parameters.
In one embodiment, the processor 130 obtains the motion profile information of the stirring rod for the stirring rod to perform two-dimensional motion in a vertical plane, and in one embodiment, the stirring rod performs two-dimensional motion in a vertical plane, including: the stirring rod repeatedly executes motion on a closed track, and the closed track is formed by a plurality of curve paths and/or line segment paths in the same vertical plane; in an embodiment, the blending parameter obtained by the processor 130 may further include at least one of an initial position of the top end of the blending rod, a speed of the blending rod moving, and a time of the blending rod moving, and the blending parameter may be preset or may be set by the user through an associated interface.
In an embodiment, when the detection methodology information is the first type of detection methodology, the processor 130 determines that the stir bar movement trajectory information is a two-dimensional movement of the stir bar in a vertical plane, where it should be noted that the initial position of the top end of the stir bar may be any point on the closed trajectory, and the movement direction of the stir bar may be always clockwise or always counterclockwise, or may alternatively be clockwise and counterclockwise; and/or when the detection methodology information is the second type of detection methodology, the motion track information of the stirring rod is that the stirring rod stops at the original position, and stirring is not executed. Specifically, in one embodiment, when the detection methodology information is a chromogenic substrate method, the processor 130 obtains a closed trajectory consisting of four line segment paths in the same vertical plane, wherein the four line segment paths are a horizontal line segment path, two vertical line segment paths respectively connected to two ends of the horizontal line segment path, and a line segment path connecting the ends of the two vertical line segments not connected to the horizontal line segment. For example, as shown in FIG. 2(a) above, which shows an example of a corresponding closed trajectory when the detection methodology information is a chromogenic substrate method, in the actual stirring process, the initial position of the tip of the stirring rod may be any point in the closed trajectory in FIG. 2(a), and the description will be given by taking the example that the initial position of the tip of the stirring rod is located at the point a in the figure and moves counterclockwise along the closed trajectory: the stirring rod moves linearly for a certain distance towards the oblique upper direction from the position of the top end of the stirring rod at the point a in the drawing, then moves linearly for a certain distance towards the vertical direction upwards, then moves linearly back towards the horizontal direction to the position right above the point a, and then moves linearly downwards towards the vertical direction to return to the point a, so that the motion of a closed track is completed. It will be appreciated that the initial position of the stirring rod tip may be at any point in fig. 2(a), and the direction of movement may also be clockwise along a closed trajectory. In one embodiment, when the detection methodology information is immunoturbidimetry, the processor 130 obtains a closed trajectory comprising two curved paths in the same vertical plane, wherein one curved path is a concave curved path and the other curved path is a convex curved path, one end of the concave curved path is connected to one end of the convex curved path, and the other end of the concave curved path is connected to the other end of the convex curved path. For example, as shown in the previous fig. 2(b) showing an example of a corresponding closed trajectory when the detection methodology information is immunoturbidimetry, in the actual stirring process, the initial position of the stirring rod tip may be any point in the closed trajectory in fig. 2(b), and the description will be given by taking the example that the initial position of the stirring rod tip is located at the point b in the figure and moves counterclockwise along the closed trajectory as an example: starting from the point b, the motion of a closed track is finished by firstly moving towards the downward direction along the path of the convex curve for a certain distance and then moving towards the upward direction along the path of the concave curve to return to the point b. It will be appreciated that the initial position of the stirring rod tip may be at any point in fig. 2(b), and the direction of movement may also be clockwise along a closed trajectory.
In order to cooperate with the stirring of the stirring rod 110, in one embodiment, the stirring mechanism further comprises a container with a non-circular cross section for containing the reaction solution, for example, referring to FIG. 3, the stirring mechanism may comprise a container with a rectangular cross section.
Example two
In one embodiment, the reaction solution is stirred according to the detection methodology information of the reaction solution. Sometimes, when a sample is to be tested, one or more reagents may be added in sequence, and after each reagent is added to the sample to form a reaction solution, it may take a while to naturally mix the reaction solution, or the reaction solution may be stirred to accelerate mixing, and then the next reagent may be added; in general, the last reagent to be added is defined as a trigger reagent, and the previous reagents are mixed reagents, so that when the test item information of the reaction solution is known, the reagents to be added to the reaction solution or the initial sample and the order therebetween can be determined, and it is possible to know which reagent is the last reagent to be added, that is, which reagent is the trigger reagent. In the second embodiment, the reaction solution is stirred by combining the detection methodology information of the reaction solution and the currently added reagent type, i.e., trigger reagent or mixed reagent, as described in detail below.
Referring to fig. 5, an embodiment of the stirring method includes steps S210 to S250.
Step S210: a stirring rod is provided for stirring the reaction solution.
Step S220: and acquiring detection methodology information of the reaction solution.
Step S230: and obtaining stirring parameters according to the acquired detection methodology information, wherein the stirring parameters at least comprise the motion track information of the stirring rod.
The stirring method of this embodiment is the same as or similar to the stirring method of the first embodiment, and for example, the step S210 is the same as or similar to the step S110, the step S220 is the same as or similar to the step S120, and the step S220 is the same as or similar to the step S120, so that these steps will not be described in detail again, and specific reference may be made to the related description in the first embodiment.
Step S240: and judging whether the reagent added into the reaction solution is the trigger reagent. In an embodiment, referring to fig. 6, step S240 may include step S241 and step S243.
Step S241: and acquiring the test item information of the reaction liquid.
Step S243: and judging whether the reagent added into the reaction solution is a trigger reagent or not according to the test item information. For example, when the test item information of the reaction solution is known, the reagents to be added to the reaction solution or the initial sample and the order therebetween can be determined, so that it is possible to know which reagent is the last reagent to be added, that is, which reagent is the trigger reagent.
It should be noted that, the steps S220 and S230 obtain the stirring parameters by obtaining the detection methodology information of the reaction solution, and they may be located before the step S240 or after the step S240, for example, when the determination result of the step S240 is that the reagent currently added to the reaction solution is the trigger reagent, the steps S220 and S230 are performed again.
Step S250: and when the reagent added into the reaction liquid at present is judged to be the trigger reagent, controlling the stirring rod to stir the reaction liquid according to the stirring parameters. For example, when the detection methodology information is the first type of detection methodology, the stirring rod is controlled to perform a two-dimensional motion in the vertical plane when it is determined that the reagent currently added to the reaction solution is the trigger reagent. In an embodiment, the stirring method may further include, when it is determined that the reagent currently added to the reaction solution is the mixed reagent, controlling the stirring rod not to perform stirring on the reaction solution, or controlling the stirring rod to perform a one-dimensional motion of a horizontal plane on the reaction solution, that is, at this time, the operation trajectory information of the stirring rod is the one-dimensional motion of the horizontal plane performed by the stirring rod, where the stirring speed of the one-dimensional motion of the horizontal plane may be a medium speed or a low speed, and one operation trajectory information of the one-dimensional motion of the horizontal plane performed by the stirring rod is shown in fig. 7.
As mentioned above, some detection methodologies are insensitive to bubbles generated during agitation but require stringent agitation times, and some detection methodologies are suitably relaxed for agitation times that are sensitive to bubbles, the requirements of these detection methodologies for the stirring process are generally embodied when the trigger reagent is added to the reaction solution at the end, when the mixed reagent is added to the reaction solution before, these requirements are generally not satisfied, and, for example, it is only necessary to mix them uniformly, and therefore, when it is judged that the currently added trigger reagent is added, the reaction solution may be stirred according to the stirring parameters acquired in step S230, when the mixed reagent is judged to be added currently, the reaction solution can be naturally mixed without stirring, or, the one-dimensional motion of the horizontal plane is simply performed to accelerate the blending, so as to save time, and of course, the stirring rod may be controlled to stir according to the stirring parameters obtained in step S230.
In one embodiment, the stirring method may further comprise providing a vessel having a non-circular cross-section for containing the reaction solution to cooperate with the stirring by the stirring rod.
The stirring method provided by the embodiment of the invention performs corresponding stirring on the reaction liquid according to the detection methodology information of the reaction liquid and whether the currently added reagent is the trigger reagent, has pertinence and strong mixing capability, and improves the test speed and the test accuracy.
In one embodiment, the in-vitro testing apparatus may include a stirring mechanism for a reaction solution (hereinafter referred to as a stirring mechanism), and referring to fig. 8, the stirring mechanism includes a stirring rod 210, a driving component 220, and a processor 230, which will be described in detail below.
The stirring rod 210 is used for stirring the reaction solution, and in one embodiment, the stirring rod 210 is a round rod made of austenitic stainless steel, and the top end of the round rod can be rounded.
The driving unit 220 is used for driving the stirring rod 210 to move. For example, taking a three-dimensional rectangular coordinate system XYZ as an example, the driving unit 220 can drive the stirring rod to perform two-dimensional motion in the horizontal X direction and the vertical Z direction. In one embodiment, the driving part 220 may be implemented using a stepping motor.
The processor 230 is configured to obtain detection methodology information of the reaction solution, and obtain stirring parameters according to the obtained detection methodology information, where the stirring parameters at least include information of a motion trajectory of the stirring rod; and judging whether the reagent added into the reaction liquid currently is a trigger reagent, and controlling the stirring rod 210 to stir the reaction liquid by controlling the driving component 220 according to the stirring parameters when the reagent added into the reaction liquid currently is judged to be the trigger reagent.
In one embodiment, the processor 230 obtains the motion profile information of the stirring rod for the stirring rod to perform two-dimensional motion in a vertical plane, and in one embodiment, the stirring rod performs two-dimensional motion in a vertical plane, including: the stirring rod repeatedly executes motion on a closed track, and the closed track is formed by a plurality of curve paths and/or line segment paths in the same vertical plane; in an embodiment, the blending parameter obtained by the processor 230 may further include at least one of an initial position of the top end of the blending rod, a speed of the blending rod moving, and a time of the blending rod moving, and the blending parameter may be preset or may be set by the user through an associated interface. In an embodiment, when the detection methodology information is the first type of detection methodology, the processor 230 determines that the stir bar has a two-dimensional motion in a vertical plane, where it should be noted that the initial position of the top end of the stir bar may be any point on the closed trajectory, and the motion direction of the stir bar may be always clockwise or always counterclockwise, or may alternatively be clockwise and counterclockwise; and/or when the detection methodology information is the second type of detection methodology, the motion track information of the stirring rod is that the stirring rod stops at the original position, and stirring is not executed. Specifically, in one embodiment, when the detection methodology information is a chromogenic substrate method, the processor 230 obtains a closed trajectory consisting of four line segment paths in the same vertical plane, wherein the four line segment paths are a horizontal line segment path, two vertical line segment paths respectively connected to two ends of the horizontal line segment path, and a line segment path connecting the ends of the two vertical line segments not connected to the horizontal line segment. For example, as shown in FIG. 2(a) above, which shows an example of a corresponding closed trajectory when the detection methodology information is a chromogenic substrate method, in the actual stirring process, the initial position of the tip of the stirring rod may be any point in the closed trajectory in FIG. 2(a), and the description will be given by taking the example that the initial position of the tip of the stirring rod is located at the point a in the figure and moves counterclockwise along the closed trajectory: the stirring rod moves linearly for a certain distance towards the oblique upper direction from the position of the top end of the stirring rod at the point a in the drawing, then moves linearly for a certain distance towards the vertical direction upwards, then moves linearly back towards the horizontal direction to the position right above the point a, and then moves linearly downwards towards the vertical direction to return to the point a, so that the motion of a closed track is completed. It will be appreciated that the initial position of the stirring rod tip may be at any point in fig. 2(a), and the direction of movement may also be clockwise along a closed trajectory. In one embodiment, when the detection methodology information is immunoturbidimetry, the processor 230 obtains a closed trajectory comprising two curved paths in the same vertical plane, wherein one curved path is a concave curved path and the other curved path is a convex curved path, one end of the concave curved path is connected to one end of the convex curved path, and the other end of the concave curved path is connected to the other end of the convex curved path. For example, as shown in the previous fig. 2(b) showing an example of a corresponding closed trajectory when the detection methodology information is immunoturbidimetry, in the actual stirring process, the initial position of the stirring rod tip may be any point in the closed trajectory in fig. 2(b), and the description will be given by taking the example that the initial position of the stirring rod tip is located at the point b in the figure and moves counterclockwise along the closed trajectory as an example: starting from the point b, the motion of a closed track is finished by firstly moving towards the downward direction along the path of the convex curve for a certain distance and then moving towards the upward direction along the path of the concave curve to return to the point b. It will be appreciated that the initial position of the stirring rod tip may be at any point in fig. 2(b), and the direction of movement may also be clockwise along a closed trajectory. In an embodiment, when the processor 230 determines that the reagent currently added to the reaction solution is the mixed reagent, the stirring rod may be controlled not to perform stirring on the reaction solution, or the stirring rod may be controlled to perform one-dimensional motion of a horizontal plane on the reaction solution.
In order to cooperate with the stirring of the stirring rod 110, in one embodiment, the stirring mechanism further comprises a container with a non-circular cross section for containing the reaction solution, for example, referring to FIG. 3, the stirring mechanism may comprise a container with a rectangular cross section.
Referring to fig. 9, a practical example of the stirring parameters is shown. In general, the above-mentioned one-dimensional motion of the horizontal plane may be a medium speed or a low speed; the stirring speed of the above-mentioned two-dimensional movement in the vertical plane may be high. In fig. 9, the mode a corresponds to the one-dimensional motion of the horizontal plane of fig. 7, and in fig. 9, the modes b and c correspond to fig. 2(a) and (b), respectively; in addition, the unit "step" in the table of fig. 9 refers to the length unit when the stirring rod is driven by the stepping motor.
The method for stirring the reaction solution of the present application may be a method for stirring the reaction solution of a blood coagulation analyzer, the stirring mechanism of the present application may be a stirring mechanism of a blood coagulation analyzer, and the in vitro assay device of the present application may be a blood coagulation analyzer.
Those skilled in the art will appreciate that all or part of the functions of the various methods in the above embodiments may be implemented by hardware, or may be implemented by computer programs. When all or part of the functions of the above embodiments are implemented by a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: a read only memory, a random access memory, a magnetic disk, an optical disk, a hard disk, etc., and the program is executed by a computer to realize the above functions. For example, the program may be stored in a memory of the device, and when the program in the memory is executed by the processor, all or part of the functions described above may be implemented. In addition, when all or part of the functions in the above embodiments are implemented by a computer program, the program may be stored in a storage medium such as a server, another computer, a magnetic disk, an optical disk, a flash disk, or a removable hard disk, and may be downloaded or copied to a memory of a local device, or may be version-updated in a system of the local device, and when the program in the memory is executed by a processor, all or part of the functions in the above embodiments may be implemented.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.
Claims (10)
1. A reaction liquid stirring method is applied to in-vitro detection equipment and is characterized by comprising the following steps:
providing a stirring rod for stirring the reaction liquid;
acquiring detection methodology information of the reaction solution;
according to the detection methodology information, obtaining stirring parameters at least including the motion trail information of the stirring rod, which specifically comprises the following steps:
when the detection methodology information is a chromogenic substrate method, the obtained stir bar motion trajectory information is that the stir bar executes motion on a closed trajectory, the closed trajectory is formed by four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths respectively connected with two ends of the horizontal line segment path, and a line segment path connecting one end of the two vertical line segments, which is not connected with one end of the horizontal line segment;
when the detection methodology information is an immunoturbidimetry method, the obtained stir bar motion trajectory information is that the stir bar executes motion on a closed trajectory, the closed trajectory is composed of two curve paths in the same vertical plane, wherein one curve path is a concave curve path, the other curve path is a convex curve path, one end of the concave curve path is connected with one end of the convex curve path, and the other end of the concave curve path is connected with the other end of the convex curve path;
and controlling the stirring rod to stir the reaction liquid according to the stirring parameters.
2. A reaction liquid stirring method is applied to in-vitro detection equipment and is characterized by comprising the following steps:
providing a stirring rod for stirring the reaction liquid;
acquiring detection methodology information of the reaction solution;
according to the detection methodology information, obtaining stirring parameters at least including the motion trail information of the stirring rod, which specifically comprises the following steps:
when the detection methodology information is a chromogenic substrate method, the obtained stir bar motion track information is that the stir bar executes motion on a closed track, and the closed track is formed by four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths respectively connected with two ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments with the end, which is not connected with the horizontal line segment, of the horizontal line segment path;
when the detection methodology information is an immunoturbidimetry method, the obtained stir bar motion trajectory information is that the stir bar executes motion on a closed trajectory, and the closed trajectory is composed of two curve paths in the same vertical plane, wherein one curve path is a concave curve path, the other curve path is a convex curve path, one end of the concave curve path is connected with one end of the convex curve path, and the other end of the concave curve path is connected with the other end of the convex curve path;
judging whether the reagent added into the reaction solution is a trigger reagent;
and when the reagent added into the reaction liquid at present is judged to be the trigger reagent, controlling the stirring rod to stir the reaction liquid according to the stirring parameters.
3. The stirring method according to claim 1 or 2, wherein the stirring rod performs a two-dimensional motion in a vertical plane, comprising: the stirring rod repeatedly executes a movement on a closed trajectory which is composed of several curved paths and/or line segment paths in the same vertical plane.
4. The stirring method according to claim 3, wherein:
when the detection methodology information is the first type of detection methodology, the stir bar motion trajectory information is the two-dimensional motion of the stir bar in the vertical plane, and the first type of detection methodology comprises an immunoturbidimetry method and a chromogenic substrate method; and/or the presence of a gas in the gas,
when the detection methodology information is the second type of detection methodology, the motion track information of the stirring rod is that the stirring rod stops at the original position and stirring is not executed, and the second type of detection methodology comprises a double magnetic circuit magnetic bead method.
5. The stirring method according to claim 2, wherein the judging whether the reagent currently added to the reaction solution is a trigger reagent comprises:
acquiring test item information of the reaction solution;
and judging whether the reagent added into the reaction solution currently is a trigger reagent or not according to the test item information.
6. The blending method of claim 1 or 2, wherein the blending parameter further comprises at least one of an initial position of a tip of the blending rod, a speed of the blending rod movement, and a time of the blending rod movement.
7. The stirring method according to claim 1 or 2, further comprising: and providing a container with a non-circular cross section for containing the reaction liquid to match the stirring of the stirring rod.
8. The utility model provides an rabbling mechanism of reaction liquid, is applied to external check out test set which characterized in that includes:
the stirring rod is used for stirring the reaction liquid;
the driving component is used for driving the stirring rod to move;
a processor for performing the blending method of any of claims 1 to 6.
9. The stirring mechanism of claim 8, further comprising a vessel of non-circular cross-section for holding the reaction solution to accommodate stirring by the stirring rod.
10. An in vitro test device comprising the agitation mechanism of claim 8 or 9.
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| CN110631964B (en) * | 2019-06-28 | 2024-02-23 | 深圳迈瑞生物医疗电子股份有限公司 | Magnetic bead method detection method and magnetic bead method detection device |
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