CN109392355A - A method of promoting Hawthorn Seeds germination - Google Patents
A method of promoting Hawthorn Seeds germination Download PDFInfo
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- CN109392355A CN109392355A CN201811544231.3A CN201811544231A CN109392355A CN 109392355 A CN109392355 A CN 109392355A CN 201811544231 A CN201811544231 A CN 201811544231A CN 109392355 A CN109392355 A CN 109392355A
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- 235000009917 Crataegus X brevipes Nutrition 0.000 title claims abstract description 56
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- 230000035784 germination Effects 0.000 title claims abstract description 40
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- 239000000843 powder Substances 0.000 claims description 12
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- 239000002202 Polyethylene glycol Substances 0.000 claims description 10
- 238000005336 cracking Methods 0.000 claims description 10
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 claims description 10
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- 229910017604 nitric acid Inorganic materials 0.000 claims description 8
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- 229940079593 drug Drugs 0.000 description 2
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- 239000003337 fertilizer Substances 0.000 description 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 2
- 239000003448 gibberellin Substances 0.000 description 2
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- 206010001928 Amenorrhoea Diseases 0.000 description 1
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- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
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- 239000003112 inhibitor Substances 0.000 description 1
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- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
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- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- SGEWCQFRYRRZDC-VPRICQMDSA-N vitexin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O SGEWCQFRYRRZDC-VPRICQMDSA-N 0.000 description 1
- PZKISQRTNNHUGF-UHFFFAOYSA-N vitexine Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O PZKISQRTNNHUGF-UHFFFAOYSA-N 0.000 description 1
- 239000010803 wood ash Substances 0.000 description 1
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- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to Chinese medicine planting technology fields, in particular to a kind of method for promoting Hawthorn Seeds germination, it is handled including Hawthorn Seeds deepfreeze, disinfection, presprouting of seeds and sowing, it shorten to the vernalization processing time of Hawthorn Seeds 6~9 days, it can germinate within after planting 15~17 days, germination percentage is 86~88%.
Description
[technical field]
The present invention relates to Chinese medicine planting technology fields, and in particular to a kind of this method for promoting Hawthorn Seeds germination.
[background technique]
Hawthorn is rosaceae hawthorn deciduous tree, is stone fruit, caryoplasm is hard, and pulp is thin, and taste is slightly sour puckery.Fruit can give birth to
Preserved fruit fruitcake is eaten or made, can be used as medicine after drying, is the distinctive dual-purpose of drug and fruit tree species of China, there is reducing blood lipid, blood pressure, heart tonifying, resist
The effects of cardiac arrhythmia, while being also the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, hernia, blood stasis full to chest diaphragm spleen,
The diseases such as amenorrhoea have good curative effect.Flavone compound Vitexin in hawthorn is a kind of stronger drug of antitumaous effect, mentions
Object is taken to have certain effect the internal growth of cancer cells of inhibition, proliferation and infiltration metastasis.
Currently, the main modes of reproduction of hawthorn has vegetative propagation and sexual propagation, vegetative propagation often with cutting propagation and is transferred
The method for connecing breeding.Propagation by grafiting is can to make stock using the seedling or axillary seedling of seminal propagation, is connect using bud or scion grafting
Or be abutted against, master is connected in bud.Vegetative propagation still be unable to do without the young plant that seminal propagation obtains.However Hawthorn Seeds belong to strong property
Seed, Germination period is long, and germination is difficult, the reason is that impermeability, the physiological dormancy phenomenon of embryo and the natural inhibitors matter of kind shell
Presence, so therefore have the title of " two-year seeds ", and germination is irregular it is generally necessary to could germinate after two winters, one "summer" player product,
Bud ratio the, this brings difficulty for hawthorn breeding and seedling propagation.
Therefore, promote Hawthorn Seeds germination, shorten its germinating time, seed germinating time is the pass of Hawthorn Seeds breeding
Key.Currently, also having about the patent for promoting Hawthorn Seeds to germinate.For example patent CN201611189461 discloses a kind of hawthorn
The germination accelerating method of seed includes the steps that immersion, refrigeration, drying, vernalization, sowing, and Hawthorn Seeds germination time can be made to shorten to 8
~9 days, germination percentage reached 86%.Patent CN201710281616 discloses a kind of vernalization side for improving Hawthorn Seeds germination percentage
Method dresses seed including fruit enzymatic hydrolysis, fruit fermentation, seed drying, seed and the method for presprouting of seeds, this method accelerates hawthorn kind
Son germination, makes it that can germinate within after planting 20~25 days unearthed, germination percentage reaches 67.4%.Patent CN201710428120 is disclosed
A kind of processing method promoting Hawthorn Seeds germination, comprising: immersions, freezing, fermentation, vernalization, the step of sowing, make hawthorn kind
The vernalization processing time of son shorten to 12~14 days, can germinate within after planting 17~19 days, germination percentage 82.7%.Although these
Method all shortens the germinating time of Hawthorn Seeds to a certain extent, and still, seed germinating time is still longer.
[summary of the invention]
In view of above content, the present invention provide it is a kind of promotion Hawthorn Seeds germination method, including deepfreeze, disinfection,
Presprouting of seeds and seed sowing shorten to the vernalization processing time of Hawthorn Seeds 6~9 days, and after planting 15~17 days i.e.
It can germinate, germination percentage is 86~88%, provides practical application value for Hawthorn Seeds breeding.
In order to achieve the above objectives, the technical solution adopted in the present invention, comprising the following steps:
1, a kind of method for promoting Hawthorn Seeds germination, which is characterized in that the described method comprises the following steps:
(1) seed deepfreeze: mature, full, no disease and pests harm the Hawthorn Seeds of picking, with rice washing water wash by rubbing with the hands completely, with
It after the wet sand of water content 60%~70% is with the weight proportion mixing of 1~3:6, is placed in refrigerator and refrigerates, cold storage procedure keeps husky
Soil is wet ventilative;
(2) seed disinfection: the seed after above-mentioned refrigeration is put into the liquor natrii hypochloritis that volumetric concentration is 5%~10%
5h~8h is impregnated, seed is pulled out, with aseptic water washing 2~3 times, drains away the water, is broken in the seed coat with needle thorn, then soaked with dilute nitric acid solution
Seed is steeped, seed is pulled out, with aseptic water washing 2~3 times;
(3) presprouting of seeds: the seed of ultrasound treatment step (2) is used in high temperature dark, seed is pulled out, seed is placed in and is urged
In bud case, under the conditions of temperature is 26~28 DEG C, humidity is 75%~85% and illumination is 100~160lx, continuous vernalization seed
4~6 days, obtain cracking seed;
(4) presprouting of seeds: the cracking seed that step (3) obtains is put into matrix, seed Surface mulch 2~3cm thickness
Matrix, then after irrigating matrix with nutrient solution, will broadcast seed-bearing matrix be placed in 26~28 DEG C of temperature, 45%~65% humidity and
It is cultivated 15~17 days in the growth cabinet of 200~250lx illumination, obtains chitting piece.
Further, in step (1), the refrigerator cold-storage temperature is -4~4 DEG C, and the processing time is 1~2 day.
Further, in step (2), the nitric acid solution concentration is 0.2~0.4mol/L, and soaking time is 8h~12h.
Further, in step (3), the ultrasonic frequency is 40~50kHz, and temperature is 60~65 DEG C, handles the time
For 40~50min.
Further, in step (4), the matrix is mainly made of the raw material of following parts by weight: 6-9 parts of fertile soil,
3-5 parts and vermiculite 3-4 parts of plant ash.
Further, in step (4), the nutrient solution is mainly blended by following components: 5~9g/L black fungus powder, 1
~5g/L polyethylene glycol and 100~300mg/L sodium Diacetate.
Black fungus powder used in the present invention is commercially available acquisition.
Compared with the prior art, the invention has the following advantages:
1, the present invention effectively facilitates Hawthorn Seeds hair by deepfreeze, seed disinfection, presprouting of seeds and sowing processing
Bud shortens germinating time, improves germination percentage, after being handled using the method for the present invention, the vernalization processing time contracting of Hawthorn Seeds
Short is 6~9 days, can be germinateed within after planting 15~17 days, and germination percentage is 86~88%.
2, the raw material of matrix of the invention is fertile soil, plant ash and vermiculite, wherein the fertile soil of raw material is rich in organic matter
With a small amount of vitamin, auxin, microelement, have it is light loose, permeable venting capability is good, and retain water and nutrients ability is strong, battalion
Feeding substance is easily absorbed by hawthorn;Plant ash is that phosphorus, calcium, magnesium, silicon, sulphur and iron rich in, manganese, copper, zinc, boron, molybdenum etc. are a large amount of
And micronutrient element, it is the disinfectant raw material that rural area is widely present, there is very strong killing pathogen effect, it can hawthorn kind
Son provides nutriment, and can prevent infecting for pathogen;Vermiculite is a kind of natural, inorganic, nontoxic minerals, can play guarantor
The multiple actions such as fertilizer, water conservation, water storage, ventilative and mineral fertilizer.By the synergistic effect of these raw materials, be conducive to as Hawthorn Seeds
Nutriment and ventilative water conservation environment are provided, and infecting for pathogen can be prevented.
3, the solution that the nutrient solution that the present invention uses is configured to for black fungus powder, polyethylene glycol and sodium Diacetate, it can have
Effect releases Hawthorn Seeds suspend mode, promotes germination, shortens germinating time.Wherein, black fungus powder can not only inhibit Hawthorn Seeds
It generates abscisic acid (abscisic acid can promote seed dormancy), moreover it is possible to which promoting seed to generate gibberellin, (gibberellin can break seed and stop
Sleep), GA content is increased in a short time, and then stimulates seed fast germination;Polyethylene glycol can improve kind under a certain concentration
The ATP content at sub- sprouting initial stage makes seed physiology activity pre-cooling, enhances various metabolic activities, thus break seed dormancy,
Seed is promoted to germinate ahead of time;Sodium Diacetate has the function of high-effect anti-mould, anti-corrosion, fresh-keeping, can reinforce black fungus powder and poly- second two
Both alcohol effect is conducive to release seed dormancy, promotes germination.Therefore, black fungus powder, polyethylene glycol and sodium Diacetate phase
Interaction, it is synergistic, promote the germination of seed.
4, the present invention uses seed cold preserving method, and low temperature promotes the after-ripening of Hawthorn Seeds physiology, and the later period is molten using sodium hypochlorite
Liquid impregnates, and plays disinfection, then uses dilute nitric acid solution immersion treatment, and kind of skin can be made to soften, and a skin permeability is planted in enhancing.In addition,
The present invention uses high temperature vernalization Hawthorn Seeds, and the mechanism of high temperature ultrasonic wave has softening tissue, improves biological membrane permeability,
Enhancing infiltration, promotes Interior Seed and extraneous mass exchange, improves metabolism, and the broken shell for being conducive to seed is sprouted.
[specific embodiment]
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Without departing substantially from spirit of that invention
In the case where essence, to modifications or substitutions made by the method for the present invention, step or condition, all belong to the scope of the present invention.
Embodiment 1:
A method of promoting Hawthorn Seeds germination, the described method comprises the following steps:
(1) seed deepfreeze: mature, full, no disease and pests harm the Hawthorn Seeds of picking, with rice washing water wash by rubbing with the hands completely, with
After the wet sand of water content 60% is with the weight proportion mixing of 1:6, it is placed in -4 DEG C of refrigerators and refrigerates 1 day, cold storage procedure keeps sandy soil
It is wet ventilative;
(2) seed disinfection: the seed after above-mentioned refrigeration is put into the liquor natrii hypochloritis that volumetric concentration is 5% and is impregnated
5h pulls seed out, with aseptic water washing 2 times, drains away the water, and is broken in the seed coat with needle thorn, then impregnated with 0.2mol/L dilute nitric acid solution
Seed 8h, pulls seed out, with aseptic water washing 2 times;
(3) presprouting of seeds: be used in high temperature dark 40kHz, at 60 DEG C ultrasound treatment step (2) seed, when processing
Between be 40min, pull seed out, seed be placed in germinating box, temperature is 26 DEG C, humidity is 75% and illumination is 100lx item
Under part, continuous vernalization seed 4 days obtains cracking seed;
(4) presprouting of seeds: the cracking seed that step (3) obtains is put into matrix, the base of seed Surface mulch 2cm thickness
Matter, then after irrigating matrix with nutrient solution, the people for seed-bearing matrix will be broadcast being placed in 26 DEG C of temperature, 45% humidity and 200lx illumination
It is cultivated 15 days in work climate box, obtains chitting piece.
Above-mentioned matrix is mainly made of the raw material of following parts by weight: 6 parts of fertile soil, 3 parts and 3 parts of vermiculite of plant ash.
Above-mentioned nutrient solution is mainly blended by following components: 5g/L black fungus powder, 1g/L polyethylene glycol and 100mg/L are bis-
Sodium acetate.
Embodiment 2:
A method of promoting Hawthorn Seeds germination, the described method comprises the following steps:
(1) seed deepfreeze: mature, full, no disease and pests harm the Hawthorn Seeds of picking, with rice washing water wash by rubbing with the hands completely, with
After the wet sand of water content 65% is with the weight proportion mixing of 1:3, it is placed in 0 DEG C of refrigerator and refrigerates 1.5 days, cold storage procedure keeps sandy soil
It is wet ventilative;
(2) seed disinfection: the seed after above-mentioned refrigeration is put into the liquor natrii hypochloritis that volumetric concentration is 7.5% and is impregnated
6.5h pulls seed out, with aseptic water washing 3 times, drains away the water, and is broken in the seed coat with needle thorn, then soaked with 0.3mol/L dilute nitric acid solution
Seed 10h is steeped, seed is pulled out, with aseptic water washing 3 times;
(3) presprouting of seeds: be used in high temperature dark 45kHz, at 62.5 DEG C ultrasound treatment step (2) seed, processing
Time is 45min, pulls seed out, and seed is placed in germinating box, temperature is 27 DEG C, humidity is 80% and illumination is 130lx
Under the conditions of, continuous vernalization seed 5 days obtains cracking seed;
(4) presprouting of seeds: the cracking seed that step (3) obtains is put into matrix, the base of seed Surface mulch 2.5cm thickness
Matter, then after irrigating matrix with nutrient solution, the people for seed-bearing matrix will be broadcast being placed in 27 DEG C of temperature, 55% humidity and 225lx illumination
It is cultivated 16 days in work climate box, obtains chitting piece.
Above-mentioned matrix is mainly made of the raw material of following parts by weight: 7.5 parts of fertile soil, 4 parts of plant ash and vermiculite 3.5
Part.
Above-mentioned nutrient solution is mainly blended by following components: 7g/L black fungus powder, 3g/L polyethylene glycol and 200mg/L are bis-
Sodium acetate.
Embodiment 3:
A method of promoting Hawthorn Seeds germination, the described method comprises the following steps:
(1) seed deepfreeze: mature, full, no disease and pests harm the Hawthorn Seeds of picking, with rice washing water wash by rubbing with the hands completely, with
After the wet sand of water content 70% is with the weight proportion mixing of 1:2, it is placed in 4 DEG C of refrigerators and refrigerates 2 days, cold storage procedure keeps sandy soil wet
Moisturize gas;
(2) seed disinfection: the seed after above-mentioned refrigeration is put into the liquor natrii hypochloritis that volumetric concentration is 10% and is impregnated
8h pulls seed out, with aseptic water washing 3 times, drains away the water, and is broken in the seed coat with needle thorn, then impregnated with 0.4mol/L dilute nitric acid solution
Seed 12h, pulls seed out, with aseptic water washing 3 times;
(3) presprouting of seeds: be used in high temperature dark 50kHz, at 65 DEG C ultrasound treatment step (2) seed, when processing
Between be 50min, pull seed out, seed be placed in germinating box, temperature is 28 DEG C, humidity is 85% and illumination is 160lx item
Under part, continuous vernalization seed 6 days obtains cracking seed;
(4) presprouting of seeds: the cracking seed that step (3) obtains is put into matrix, the base of seed Surface mulch 3cm thickness
Matter, then after irrigating matrix with nutrient solution, the people for seed-bearing matrix will be broadcast being placed in 28 DEG C of temperature, 65% humidity and 250lx illumination
It is cultivated 17 days in work climate box, obtains chitting piece.
Above-mentioned matrix is mainly made of the raw material of following parts by weight: 9 parts of fertile soil, 5 parts and 4 parts of vermiculite of plant ash.
Above-mentioned nutrient solution is mainly blended by following components: 9g/L black fungus powder, 5g/L polyethylene glycol and 300mg/L are bis-
Sodium acetate.
Control group 1:
Other steps are identical with example 1, but step (1) Hawthorn Seeds are without -4 DEG C of refrigerator sand storages.
Control group 2:
Other steps are identical with example 1, but the composition of raw materials of step (4) matrix are as follows: 3 parts of plant ash and 3 portions of leech
Stone.
Control group 3:
Other steps are identical with example 1, but the composition of raw materials of step (4) matrix are as follows: 6 parts of fertile soil and 3 portions of leech
Stone.
Control group 4:
Other steps are identical with example 1, but the composition of raw materials of step (4) matrix are as follows: 6 parts of fertile soil and 3 parts of grass
Wood ash.
Control group 5:
Other steps are identical with example 1, but the component of step (4) nutrient solution are as follows: 1g/L polyethylene glycol and
100mg/L sodium Diacetate.
Control group 6:
Other steps are identical with example 1, but the component of step (4) nutrient solution are as follows: 5g/L black fungus powder and
100mg/L sodium Diacetate.
Control group 7:
Other steps are identical with example 1, but the component of step (4) nutrient solution are as follows: 5g/L black fungus powder and 1g/L
Polyethylene glycol.
Control group 8:
Hawthorn Seeds germination accelerating method is referring to a kind of " the processing side for promoting Hawthorn Seeds germination patent CN201710428120
Method ".
Above-described embodiment and control group handle 1100 Hawthorn Seeds respectively, are randomly divided into 11 groups, every group of 100 hawthorn kinds
Son carries out vernalization to the Hawthorn Seeds of embodiment and control group respectively, is sowed after vernalization.
Testing experiment:
Each embodiment of pilot survey and the seed after the continuous vernalization of control group 6 days show money or valuables one carries unintentionally grain number, hawthorn kind after sowing 17 days
The unearthed strain number of son, calculates germination percentage, is specifically shown in Table 1.
Table 1
Group | Seed grain number (grain) | The seed number that shows money or valuables one carries unintentionally (grain) | Germination, which is unearthed, counts (strain) |
Embodiment 1 | 100 | 50 | 86 |
Embodiment 2 | 100 | 49 | 88 |
Embodiment 3 | 100 | 51 | 87 |
Control group 1 | 100 | 32 | 63 |
Control group 2 | 100 | 31 | 65 |
Control group 3 | 100 | 36 | 70 |
Control group 4 | 100 | 32 | 73 |
Control group 5 | 100 | 28 | 62 |
Control group 6 | 100 | 33 | 68 |
Control group 7 | 100 | 34 | 67 |
Control group 8 | 100 | 38 | 79 |
As seen from the above table, step of the invention (4) is an indivisible entirety, is mutually cooperateed between component, jointly
Effect, the dosage of component can not arbitrarily increase and decrease;Using method of the invention, the vernalization of Hawthorn Seeds can effectively be made to handle time contracting
Short is 6~9 days, can be germinateed within after planting 15~17 days, and germination percentage is 86~88%.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (6)
1. a kind of method for promoting Hawthorn Seeds germination, which is characterized in that the described method comprises the following steps:
(1) seed deepfreeze: mature, full, no disease and pests harm the Hawthorn Seeds of picking are washed by rubbing with the hands completely with rice washing water, and aqueous
After the wet sand of amount 60%~70% is with the weight proportion mixing of 1~3:6, it is placed in refrigerator and refrigerates, cold storage procedure keeps sandy soil wet
Moisturize gas;
(2) seed disinfection: the seed after above-mentioned refrigeration is put into the liquor natrii hypochloritis that volumetric concentration is 5%~10% and is impregnated
5h~8h pulls seed out, with aseptic water washing 2~3 times, drains away the water, and is broken in the seed coat with needle thorn, then impregnate kind with dilute nitric acid solution
Son pulls seed out, with aseptic water washing 2~3 times;
(3) presprouting of seeds: the seed of ultrasound treatment step (2) is used in high temperature dark, seed is pulled out, seed is placed in germinating box
In, under the conditions of temperature is 26~28 DEG C, humidity is 75%~85% and illumination is 100~160lx, continuous vernalization seed 4~6
It, obtains cracking seed;
(4) presprouting of seeds: the cracking seed that step (3) obtains is put into matrix, the matrix of seed Surface mulch 2~3cm thickness,
After irrigating matrix with nutrient solution again, will broadcast seed-bearing matrix be placed in 26~28 DEG C of temperature, 45%~65% humidity and 200~
It is cultivated 15~17 days in the growth cabinet of 250lx illumination, obtains chitting piece.
2. a kind of method for promoting Hawthorn Seeds germination according to claim 1, which is characterized in that described in step (1)
Refrigerator cold-storage temperature is -4~4 DEG C, and the processing time is 1~2 day.
3. a kind of method for promoting Hawthorn Seeds germination according to claim 1, which is characterized in that described in step (2)
Nitric acid solution concentration is 0.2~0.4mol/L, and soaking time is 8h~12h.
4. a kind of method for promoting Hawthorn Seeds germination according to claim 1, which is characterized in that described in step (3)
Ultrasonic frequency is 40~50kHz, and temperature is 60~65 DEG C, and the processing time is 40~50min.
5. a kind of method for promoting Hawthorn Seeds germination according to claim 1, which is characterized in that described in step (4)
Matrix is mainly made of the raw material of following parts by weight: 6-9 parts of fertile soil, 3-5 parts and vermiculite 3-4 parts of plant ash.
6. a kind of method for promoting Hawthorn Seeds germination according to claim 1, which is characterized in that described in step (4)
Nutrient solution is mainly blended by following components: 5~9g/L black fungus powder, 1~5g/L polyethylene glycol and 100~300mg/L are bis-
Sodium acetate.
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CN106508183A (en) * | 2016-12-21 | 2017-03-22 | 阜阳市颍泉区玉寿种植专业合作社 | Germination accelerating method for fructus crataegi seeds |
CN106912218A (en) * | 2017-04-26 | 2017-07-04 | 蚌埠市宝煦家庭农场 | A kind of germination accelerating method for improving Hawthorn Seeds germination percentage |
CN107266241A (en) * | 2017-06-08 | 2017-10-20 | 李斌 | A kind of processing method for promoting Hawthorn Seeds to germinate |
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CN106508183A (en) * | 2016-12-21 | 2017-03-22 | 阜阳市颍泉区玉寿种植专业合作社 | Germination accelerating method for fructus crataegi seeds |
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