CN109355346B - Method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace - Google Patents
Method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 62
- 230000004151 fermentation Effects 0.000 title claims abstract description 62
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 title claims abstract description 42
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 239000002509 fulvic acid Substances 0.000 title claims abstract description 42
- 229940095100 fulvic acid Drugs 0.000 title claims abstract description 42
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- 238000000034 method Methods 0.000 claims abstract description 10
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- 239000001963 growth medium Substances 0.000 claims description 31
- 238000012258 culturing Methods 0.000 claims description 24
- 244000061456 Solanum tuberosum Species 0.000 claims description 14
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 13
- 239000008103 glucose Substances 0.000 claims description 13
- 230000000813 microbial effect Effects 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
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- 108010080698 Peptones Proteins 0.000 claims description 11
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- 241000589516 Pseudomonas Species 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 238000013329 compounding Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 abstract description 4
- 239000002068 microbial inoculum Substances 0.000 abstract description 3
- 239000000758 substrate Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 16
- 239000002689 soil Substances 0.000 description 7
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 6
- 239000011790 ferrous sulphate Substances 0.000 description 4
- 235000003891 ferrous sulphate Nutrition 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 4
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- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 240000005020 Acaciella glauca Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 244000052158 Eurya japonica Species 0.000 description 1
- 235000018822 Eurya japonica Nutrition 0.000 description 1
- 241000218652 Larix Species 0.000 description 1
- 235000005590 Larix decidua Nutrition 0.000 description 1
- 241000018650 Pinus massoniana Species 0.000 description 1
- 235000008582 Pinus sylvestris Nutrition 0.000 description 1
- 235000011610 Pinus tabuliformis Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 240000003243 Thuja occidentalis Species 0.000 description 1
- 235000008109 Thuja occidentalis Nutrition 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
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- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 239000003077 lignite Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000001839 pinus sylvestris Substances 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a method for producing biochemical fulvic acid by semisolid fermentation of apple pomace, which comprises the steps of inoculating various fermentation strains subjected to expanded culture into waste pomace, carrying out semisolid fermentation to produce biochemical fulvic acid, and preparing biochemical fulvic acid solution by taking the inoculation amount of a mixed microbial inoculum, the addition amount of urea, the water content of a fermentation substrate and the fermentation time as variables in the fermentation process. The method can reduce the burden of related apple enterprises, protect the environment, reduce the cost for producing the biochemical fulvic acid, use the optimal fermentation process, prepare the biochemical fulvic acid solution with multifunction, high efficiency and wide application, and has practical reference significance for producing the biochemical fulvic acid by semi-solid fermentation.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace.
Background
The fulvic acid is the part of humic acid substances with water solubility and highest activity, the original fulvic acid is extracted from the humic acid, but a large amount of chemical substances such as acid, alkali and the like are consumed, and some of the fulvic acid also needs high temperature, high pressure and special equipment, consumes a large amount of energy, generates waste water and waste gas, causes pollution to the environment and has higher production cost. The fulvic acid is extracted from renewable resources such as agricultural and forestry wastes by the microbial fermentation technology through the biotechnology inoculation of fast-rotting bacteria, composting, fermentation, chemical extraction technology and the like, and is also called biochemical fulvic acid. Compared with natural fulvic acid extracted from peat and lignite, the biochemical fulvic acid has the advantages of small molecular weight, good water solubility, high biological activity, acid and alkali resistance, divalent ion resistance, no flocculation under various water qualities, and good co-solubility with N, P, K and trace elements.
The production and application of biochemical fulvic acid are brisk and developed from the end of the eighties of the last century. Research reports that after the biochemical fulvic acid is used for treating soil, the aggregate content of the soil with the particle size of more than 0.25mm is increased by 8.5-20.0%, which shows that the biochemical fulvic acid fertilizer has the effect of obviously improving the structural performance of the soil. The biochemical fulvic acid has stronger physiological activity, has promotion effect on the development of root systems and C, N and water metabolism in plants, and simultaneously improves the chemical property of soil and activates soil nutrients by enhancing the microbial activity of the soil, so that the nutrient elements such as N, P, K and the like are gradually released in a complex state to improve the utilization rate of the fertilizer. The biochemical fulvic acid can also improve the drought resistance of crops, increase the number of beneficial microorganisms such as ammoniation bacteria, ammonia-fixing bacteria and fiber decomposition bacteria in soil, increase the chlorophyll content of plants and the like.
In the aspect of forestry, biochemical fulvic acid solutions with different concentrations are used for soaking roots of Chinese pine, pinus sylvestris, larch and spruce, so that the afforestation survival rate can be improved by 11.9-16.3%; the survival rate of the spruce and the Eurya japonica can be respectively improved by 11.5 percent and 10.5 percent through root irrigation. The experiment of the biochemical fulvic acid-alkali-modified fertilizer in western forest cultivation shows that: when the seedling is used for the seedlings of Xinjiang poplar, the height and the ground diameter of the seedlings are respectively increased by 49cm and 0.49cm compared with the contrast, the survival rate is improved by 8.7 percent, the growth amount of new shoots is increased by 3.7cm, and when the seedling is used for the young forest of spruce and arborvitae, the average annual plant height is respectively increased by 2.9 cm and 4.9cm compared with the contrast; the fertilizer can promote the growth of nursery stock and young tree, raise afforestation survival rate and improve nursery and afforestation land.
The biochemical fulvic acid is produced by fermenting the crop wastes, the wastes can be utilized, the environment cannot be polluted, the raw materials are easy to obtain, the cost is low, the environment cannot be polluted in the production and use processes, and the product can show the advantages of multifunction, high efficiency, wide applicability and the like in the agriculture and forestry production, and is a novel promising biotechnology for sustainable development of ecological agriculture and forestry.
Disclosure of Invention
The invention provides a method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace with low cost and scientific proportion.
The invention provides a method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace, which comprises the following steps:
(1) preparing a fermentation culture medium with 40-80% of water content by taking apple pomace as a fermentation raw material;
(2) mixing 20g of glucose, 200g of potato, 15-20g of agar and 1000ml of water, fully stirring to prepare a potato glucose culture medium with natural pH, mixing 20g of glucose, 100g of potato, 15g of agar and 1000ml of water, fully stirring to prepare a bean sprout juice culture medium with pH of 7.2-7.4, mixing 10g of peptone, 3g of beef extract, 5g of calcium chloride, 15-20g of agar and 1000ml of water, and fully stirring to prepare a beef extract peptone culture medium with pH of 7.0-7.2;
(3) culturing Aspergillus niger with potato glucose culture medium at 28 deg.C for 48h, culturing Saccharomyces cerevisiae and Candida utilis with bean sprout juice culture medium for 24h, culturing Bacillus subtilis and Pseudomonas bacteria with beef extract peptone culture medium at 30 deg.C for 24h, and activating the above five strains;
(4) compounding the activated aspergillus niger, candida utilis, saccharomyces cerevisiae, bacillus subtilis and pseudomonas in the step (3) according to the inoculation ratio of 1:1:1:2:2, and culturing the compounded mixture in an amplification culture medium to prepare the mixed microbial agent, wherein the culture process of the amplification culture medium is as follows: culturing mould in a shaking table at a constant temperature of 30 ℃ for 24h at a rotating speed of 220rpm, culturing yeast in the shaking table at a constant temperature of 30 ℃ for 12h at a rotating speed of 180rpm, culturing bacteria in the shaking table at a constant temperature of 30 ℃ for 12h at a rotating speed of 180rpm, culturing the five strains to obtain corresponding seed solutions, taking 10% of the seed solutions, carrying out amplification culture in a fermentation bottle for 48h to obtain fermentation liquor, mixing the fermentation liquor and sterilized bran in a ratio of 1:1, fermenting, and airing for 1d-3d to prepare a mixed microbial agent;
(5) and (2) inoculating a certain amount of mixed microbial agent and a certain amount of urea into the fermentation medium obtained in the step (1) for fermentation for a period of time, and separating pulp from residues after fermentation to obtain fermentation liquor, namely the biochemical fulvic acid solution.
Specifically, the above-mentioned expanding medium refers to a potato glucose medium, a bean sprout juice medium and a beef extract peptone medium, which are the remainders of agar, after removal of agar.
Particularly, the inoculation amount of the mixed microbial agent is 3-7%.
In particular, the amount of urea is 1% to 3%.
In particular, the fermentation time in the fermentation medium is 20d-35 d.
Various fermentation strains after enlarged culture are inoculated into the waste fruit residues, semisolid fermentation is carried out to produce the biochemical fulvic acid, and the biochemical fulvic acid solution is prepared by taking the inoculation amount of the mixed microbial inoculum, the addition amount of urea, the water content of a fermentation substrate and the fermentation time as variables in the fermentation process. The method can reduce the burden of related apple enterprises, protect the environment, reduce the cost for producing the biochemical fulvic acid, use the optimal fermentation process, prepare the biochemical fulvic acid solution with multifunction, high efficiency and wide application, and has practical reference significance for producing the biochemical fulvic acid by semi-solid fermentation.
Detailed Description
For a further understanding of the invention, reference will now be made to the preferred embodiments of the invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the invention, and not to limit the scope of the claims.
Example one
A method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace comprises the following steps:
(1) preparing a fermentation culture medium with 40-80% of water content by taking apple pomace as a fermentation raw material;
(2) mixing 20g of glucose, 200g of potato, 15-20g of agar and 1000ml of water, fully stirring to prepare a potato glucose culture medium with natural pH, mixing 20g of glucose, 100g of potato, 15g of agar and 1000ml of water, fully stirring to prepare a bean sprout juice culture medium with pH of 7.2-7.4, mixing 10g of peptone, 3g of beef extract, 5g of calcium chloride, 15-20g of agar and 1000ml of water, and fully stirring to prepare a beef extract peptone culture medium with pH of 7.0-7.2;
(3) culturing Aspergillus niger with potato glucose culture medium at 28 deg.C for 48h, culturing Saccharomyces cerevisiae and Candida utilis with bean sprout juice culture medium for 24h, culturing Bacillus subtilis and Pseudomonas bacteria with beef extract peptone culture medium at 30 deg.C for 24h, and activating the above five strains;
(4) compounding the activated aspergillus niger, candida utilis, saccharomyces cerevisiae, bacillus subtilis and pseudomonas in the step (3) according to the inoculation ratio of 1:1:1:2:2, and culturing the compounded mixture in an amplification culture medium to prepare the mixed microbial agent, wherein the culture process of the amplification culture medium is as follows: culturing mould in a shaking table at a constant temperature of 30 ℃ for 24h at a rotating speed of 220rpm, culturing yeast in the shaking table at a constant temperature of 30 ℃ for 12h at a rotating speed of 180rpm, culturing bacteria in the shaking table at a constant temperature of 30 ℃ for 12h at a rotating speed of 180rpm, culturing the five strains to obtain corresponding seed solutions, taking 10% of the seed solutions, carrying out amplification culture in a fermentation bottle for 48h to obtain fermentation liquor, mixing the fermentation liquor and sterilized bran in a ratio of 1:1, fermenting, and airing for 1d-3d to prepare a mixed microbial agent;
(5) and (2) inoculating 3-7% of the inoculum size of the mixed microbial agent and 1-3% of the added amount of urea into the fermentation medium in the step (1) for fermentation for 20-35 d, and separating pulp and slag after fermentation to obtain fermentation liquid, namely the biochemical fulvic acid solution.
And (3) measuring the content of the fulvic acid by using biochemical fulvic acid solutions prepared according to different mixed microbial inoculum sizes, different urea adding amounts and different fermentation medium fermentation times. The determination method is a potassium dichromate volumetric method, and the operation method is as follows:
placing 0.2g sample in 250ml conical flask, adding distilled water, inserting small glass funnel on the bottle mouth, heating in boiling water under stirring for 30min, taking out, cooling, pouring all the solution and residue into 100ml volumetric flask, adding distilled water to scale and shaking up, and filtering residue with medium speed filter paper to obtain filtrate. 5ml of the above filtrate was taken and put into a 250ml conical flask, 5ml of a 4.8mol/L potassium dichromate solution was added, 15ml of concentrated sulfuric acid was added, and the mixture was heated in boiling water for 30min to oxidize the potassium dichromate solution. Finally, the solution obtained by oxidation is taken out from the boiling water bath, cooled to room temperature, 70ml of distilled water and 3 drops of the phenanthroline indicator are added into the solution, titration is carried out by using a ferrous sulfate standard solution, and the titration is stopped when the solution changes from orange to green to brick red. The formula for calculating the content of fulvic acid is as follows:
in the formula: v 0 The volume of ferrous sulfate consumed in a titration blank tube is measured; v is ferrous sulfate consumed in titration sample tubeAccumulating; n refers to the molar concentration of the ferrous sulfate standard solution; c is the carbon ratio of fulvic acid (generally 0.5); g is the mass of the sample added during the preparation of the sample solution; a refers to the volume of sample solution titrated; b refers to the volume of sample solution drawn at the time of the drip.
The results are shown in table 1, and it can be seen that the inoculation amount of 7%, the water content of the fermentation substrate of 50%, the urea addition amount of 2% and the fermentation time of 30 days are optimal processes, and the content of fulvic acid in the fermentation product exceeds 20%.
TABLE 1
Claims (3)
1. A method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace comprises the following steps:
(1) preparing a fermentation culture medium with 40-80% of water content by taking apple pomace as a fermentation raw material;
(2) mixing 20g of glucose, 200g of potato, 15-20g of agar and 1000ml of water, fully stirring to prepare a potato glucose culture medium with natural pH, mixing 20g of glucose, 100g of potato, 15g of agar and 1000ml of water, fully stirring to prepare a bean sprout juice culture medium with pH of 7.2-7.4, mixing 10g of peptone, 3g of beef extract, 5g of calcium chloride, 15-20g of agar and 1000ml of water, and fully stirring to prepare a beef extract peptone culture medium with pH of 7.0-7.2;
(3) culturing Aspergillus niger with potato glucose culture medium at 28 deg.C for 48h, culturing Saccharomyces cerevisiae and Candida utilis with bean sprout juice culture medium for 24h, culturing Bacillus subtilis and Pseudomonas bacteria with beef extract peptone culture medium at 30 deg.C for 24h, and activating the above five strains;
(4) compounding the activated aspergillus niger, candida utilis, saccharomyces cerevisiae, bacillus subtilis and pseudomonas in the step (3) according to the inoculation ratio of 1:1:1:2:2, and culturing the compounded mixture in an amplification culture medium to prepare the mixed microbial agent, wherein the culture process of the amplification culture medium is as follows: culturing mould in a shaking table at a constant temperature of 30 ℃ for 24h at a rotating speed of 220rpm, culturing yeast in the shaking table at a constant temperature of 30 ℃ for 12h at a rotating speed of 180rpm, culturing bacteria in the shaking table at a constant temperature of 30 ℃ for 12h at a rotating speed of 180rpm, culturing the five strains to obtain corresponding seed solutions, taking 10% of the seed solutions, carrying out amplification culture in a fermentation bottle for 48h to obtain fermentation liquor, mixing the fermentation liquor and sterilized bran in a ratio of 1:1, fermenting, and airing for 1d-3d to prepare a mixed microbial agent;
(5) inoculating a certain amount of mixed microbial agent and a certain amount of urea into the fermentation medium obtained in the step (1) for fermentation for a period of time, and separating pulp from residues after fermentation to obtain fermentation liquor, namely the biochemical fulvic acid solution;
the inoculation amount of the mixed microbial agent is 3% -7%; the addition amount of the urea is 1% -3%.
2. The method for producing biochemical fulvic acid through semi-solid fermentation of apple pomace according to claim 1, comprising the following steps of: the expanding culture medium refers to the residual part of potato glucose culture medium, bean sprout juice culture medium and beef extract peptone culture medium after agar is removed.
3. The method for producing biochemical fulvic acid through apple pomace semi-solid fermentation according to claim 1, is characterized in that: the fermentation time in the fermentation medium is 20d-35 d.
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| CN1974490A (en) * | 2006-12-11 | 2007-06-06 | 陈五岭 | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure |
| CN107574136A (en) * | 2017-10-30 | 2018-01-12 | 中国水产科学研究院淡水渔业研究中心 | A kind of preparation method of semi-solid probiotics used for aquiculture |
| CN108587944A (en) * | 2018-04-04 | 2018-09-28 | 北京航天恒丰科技股份有限公司 | A kind of composite bacteria agent and preparation method thereof for the fermentation of agricultural waste gurry |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1974490A (en) * | 2006-12-11 | 2007-06-06 | 陈五岭 | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure |
| CN107574136A (en) * | 2017-10-30 | 2018-01-12 | 中国水产科学研究院淡水渔业研究中心 | A kind of preparation method of semi-solid probiotics used for aquiculture |
| CN108587944A (en) * | 2018-04-04 | 2018-09-28 | 北京航天恒丰科技股份有限公司 | A kind of composite bacteria agent and preparation method thereof for the fermentation of agricultural waste gurry |
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Effective date of registration: 20221130 Address after: 337000 Ma Shan Zhen Xiao Qiao Cun, Xiangdong District, Pingxiang City, Jiangxi Province Patentee after: Jiangxi Sanhui Technology Co.,Ltd. Address before: 337000 Ma Shan Zhen Xiao Qiao Cun, Xiangdong District, Pingxiang City, Jiangxi Province Patentee before: PINGXIANG LELE HUMIC ACID FACTORY |