CN109355189A - Protozoic method is removed when a kind of microdisk electrode - Google Patents
Protozoic method is removed when a kind of microdisk electrode Download PDFInfo
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- CN109355189A CN109355189A CN201811287668.3A CN201811287668A CN109355189A CN 109355189 A CN109355189 A CN 109355189A CN 201811287668 A CN201811287668 A CN 201811287668A CN 109355189 A CN109355189 A CN 109355189A
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- microdisk electrode
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- culturing microalgae
- protozoic
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- 238000000034 method Methods 0.000 title claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 81
- 238000012258 culturing Methods 0.000 claims abstract description 64
- 238000000605 extraction Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims description 9
- 230000000694 effects Effects 0.000 abstract description 9
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 230000005791 algae growth Effects 0.000 abstract description 6
- 239000000284 extract Substances 0.000 abstract description 2
- 230000000241 respiratory effect Effects 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 36
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000009792 diffusion process Methods 0.000 description 4
- 230000029058 respiratory gaseous exchange Effects 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000700141 Rotifera Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to both culturing microalgae technical field, when specifically a kind of microdisk electrode, removes protozoic method.The present invention extracts the water in microdisk electrode slot by (1), and is passed into high concentrations of gas dissolver；(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains the both culturing microalgae water of high CO concentration；(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot, the effect that CO sufficiently inhibits protozoic respiratory enzyme is given full play to, keep protozoan dead, and sufficiently promote micro algae growth, improve microalgae density, improve the overall efficiency of microdisk electrode, be it is a kind of can effectively remove protozoan, and microalgae cell will not be caused damage microdisk electrode when remove protozoic method.
Description
Technical field
The present invention relates to both culturing microalgae technical field, when specifically a kind of microdisk electrode, removes protozoic method.
Background technique
Outdoors during both culturing microalgae, protozoan present in natural environment can pass through water body and various cultivation
It is operated into culture solution, these protozoans are under the action of microalgae is discharged into the organic matter of its own secretion in culture solution
It can largely be proliferated, microalgae is also resulted in when serious can not normal growth.
Currently in order to the protozoan in culture solution can be killed, usually using physical filtering method, CO is filled2Method, hydrochloric acid
Acidization, raising salinity method etc., but insect killing effect is poor, therefore, typically now in low-density (OD3 or less) microalga cultivation process
In, added in cell liquid the chemicals with oxidisability such as 10-20PPM hypochlorous acid below or hydrogen peroxide to protozoan into
Row is killed, but due to the oxidisability non-selectivity of hypochlorous acid or hydrogen peroxide, allows for moving using high dose chemicals to primary
Microalgae cell is caused to damage while object is killed, so as to cause the decline of microalgae yield.Also, it kills protozoic
Effect is also influenced by frustule concentration in culture solution and its secretion concentration, and cell concentration is bigger, and secretion is more, due to
Frustule and its secretion are organic matters, can be reacted with the insecticide in cell liquid, so can also directly result in work
Dosage for protozoic insecticide is reduced, to reduce to protozoic lethality.
Therefore, find it is a kind of can effectively remove protozoan, and microalgae cell will not be caused damage microdisk electrode
When to remove protozoic method be the task of top priority.
Summary of the invention
In order to solve the above technical problems existing in the prior art, it is removed when the present invention provides a kind of microdisk electrode primary
The method of animal, specific as follows:
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) water in microdisk electrode slot is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, it is dense obtains high CO
The both culturing microalgae water of degree；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
Preferably, the both culturing microalgae water of the high CO concentration is CO concentration >=40ppm both culturing microalgae water.It is further excellent
Choosing, the both culturing microalgae water of the high CO concentration is CO concentration >=60ppm both culturing microalgae water.It is further preferred that described
The both culturing microalgae water of high CO concentration is the both culturing microalgae water that CO concentration is 80ppm.In both culturing microalgae water when CO concentration >=40ppm
Protozoic breathing enzyme effect can preferably be inhibited, keep protozoan dead；In both culturing microalgae water when CO concentration >=60ppm
Protozoic breathing enzyme effect can sufficiently be inhibited, keep whole protozoans dead；CO concentration is 80ppm in both culturing microalgae water
When, it can not only sufficiently inhibit protozoic breathing enzyme effect, keep whole protozoans dead, moreover it is possible to it is raw to sufficiently promote microalgae
It is long, improve microalgae density.
Preferably, in the step (3), after the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot,
It is stirred for uniformly, spreading it sufficiently.It is stirred for uniformly, its abundant diffusion energy being made to improve both culturing microalgae water entirety rapidly
CO concentration, kills rapidly protozoan.
Preferably, the step (1), (2), (3) are to recycle progress simultaneously.Step (1), (2), (3) recycle progress simultaneously
The stable level in microdisk electrode slot can be effectively kept, water-level fluctuation, more conducively micro algae growth is reduced, helps to improve microalgae
Density, continuity is also preferable, industrialization preferably.
Preferably, during the step (1), (2), (3), the CO concentration 60- of cultivation water in microdisk electrode slot is controlled
80ppm.Protozoic breathing enzyme effect can not only sufficiently be inhibited at this time, keep whole protozoans dead, moreover it is possible to sufficiently promote
Into micro algae growth, microalgae density is improved.
Preferably, during the step (1), (2), (3), filtration step is added.It is further preferred that the mistake
Filter, is the strainer filtering with mesh less than 60 μm.It is former that this strainer filtering with mesh less than 60 μm can remove the large size in water body
Lively object is further promoted and removes protozoic speed and effect.
Preferably, during the step (1), (2), (3), the gas to shed in both culturing microalgae water is collected.One oxidation
Carbon plays the role of promotion to micro algae growth, but be to higher plant and animal it is toxic, removing protozoic process with it
In, a small amount of diffusion is had, though diffusing capacity is less, is in harmless state, but still is reused after removing or separate after should collecting
Preferably.Wheel animalcule, protozoan, Escherichia coli are as follows through carbon monoxide processing acquired results:
The pesticide-germicide of CO acts on
After 10 hours, most protozoan and wheel animalcule are dead, and pollution is reduced, and the quantity of Escherichia coli also has by a relatively large margin
Reduction, and the reproductive capacity of Escherichia coli is also deteriorated.
Compared with prior art, the technical effect of the invention is embodied in:
The present invention extracts the water in microdisk electrode slot by (1), and is passed into high concentrations of gas dissolver；(2)
CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains the both culturing microalgae of high CO concentration
Water；(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot, has given full play to CO and has sufficiently inhibited primary dynamic
It is dead to make protozoan, and sufficiently promotes micro algae growth for the effect of the respiratory enzyme of object, improves microalgae density, improves microalgae training
Feeding overall efficiency, be it is a kind of can effectively remove protozoan, and microalgae cell will not be caused damage microdisk electrode when
Remove protozoic method.
Detailed description of the invention
Fig. 1 is method schematic diagram of the invention.
Fig. 2 is method schematic diagram of the invention.
Specific embodiment
It is limited below with reference to specific embodiment technical solution of the present invention is further, but claimed
Range is not only limited to made description.
Embodiment 1
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=60ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
Embodiment 2
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/3 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=40ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
Embodiment 3
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/5 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=80ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
Embodiment 4
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=60ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot, is stirred for uniformly, making it sufficiently
Diffusion.
Embodiment 5
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=60ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
The step (1), (2), (3), while recycling progress.
Embodiment 6
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=80ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
During the step (1), (2), (3), the CO concentration 60-80ppm of cultivation water in microdisk electrode slot is controlled.
Embodiment 7
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=60ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
60 μm of mesh of strainer filtering is used during the step (1), (2), (3).
Embodiment 8
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=60ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
During the step (1), (2), (3), the gas to shed in both culturing microalgae water is collected.
Embodiment 9
Protozoic method is removed when a kind of microdisk electrode, is included the following steps:
(1) in microdisk electrode slot 1/4 water is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains CO concentration
The both culturing microalgae water of=80ppm；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot, is stirred for uniformly, making it sufficiently
Diffusion.
During the step (1), (2), (3), the CO concentration 60-80ppm of cultivation water in microdisk electrode slot is controlled.
The step (1), (2), (3), while recycling progress.
60 μm of mesh of strainer filtering is used during the step (1), (2), (3).
During the step (1), (2), (3), the gas to shed in both culturing microalgae water is collected.
The microdisk electrode slot of 10 30L is set, is packed into 20L liquid wherein respectively, culture microalgae to microalgae density is kept
Stablize, compiling is No. 1-10.
6h is handled to 1-9 microdisk electrode slot using the method for embodiment 1-9, No. 10 do not deal with, as a control group, so
After compare, acquired results are as follows:
From the foregoing, it will be observed that method of the invention can effectively make protozoan death, and micro algae growth is sufficiently promoted, improves microalgae
Density improves the overall efficiency of microdisk electrode.
Finally it is pointed out that above embodiments are only the more representational examples of the present invention.Obviously, technology of the invention
Scheme is not limited to above-described embodiment, and acceptable there are many deformations.Those skilled in the art can be from disclosed by the invention
All deformations that content is directly exported or associated, are considered as protection scope of the present invention.
Claims (10)
1. removing protozoic method when a kind of microdisk electrode, which comprises the steps of:
(1) water in microdisk electrode slot is extracted, and is passed into high concentrations of gas dissolver；
(2) CO gas is dissolved into the both culturing microalgae water of extraction by high concentrations of gas dissolver, obtains high CO concentration
Both culturing microalgae water；
(3) the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot.
2. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the high CO concentration
Both culturing microalgae water, be CO concentration >=40ppm both culturing microalgae water.
3. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the high CO concentration
Both culturing microalgae water, be CO concentration >=60ppm both culturing microalgae water.
4. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the high CO concentration
Both culturing microalgae water, be CO concentration be 80ppm both culturing microalgae water.
5. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the step (3)
In, after the both culturing microalgae water of obtained high CO concentration is passed through in microdisk electrode slot, it is stirred for uniformly, spreading it sufficiently.
6. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the step (1),
(2), (3) are to recycle progress simultaneously.
7. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the step (1),
(2), during (3), the CO concentration 60-80ppm of cultivation water in microdisk electrode slot is controlled.
8. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the step (1),
(2), during (3), filtration step is added.
9. removing protozoic method when microdisk electrode according to claim 8, which is characterized in that the filtering is
Strainer filtering with mesh less than 60 μm.
10. removing protozoic method when microdisk electrode according to claim 1, which is characterized in that the step
(1), during (2), (3), the gas to shed in both culturing microalgae water is collected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811287668.3A CN109355189B (en) | 2018-10-31 | 2018-10-31 | Method for removing protozoa during microalgae culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811287668.3A CN109355189B (en) | 2018-10-31 | 2018-10-31 | Method for removing protozoa during microalgae culture |
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| CN109355189A true CN109355189A (en) | 2019-02-19 |
| CN109355189B CN109355189B (en) | 2022-09-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115537339A (en) * | 2022-09-22 | 2022-12-30 | 天津长芦汉沽盐场有限责任公司 | A kind of method of salina outdoor breeding to reduce insect damage |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN104593263A (en) * | 2015-01-13 | 2015-05-06 | 新奥科技发展有限公司 | Method for treating protozoon pollution in microalgae culture |
-
2018
- 2018-10-31 CN CN201811287668.3A patent/CN109355189B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN104593263A (en) * | 2015-01-13 | 2015-05-06 | 新奥科技发展有限公司 | Method for treating protozoon pollution in microalgae culture |
Non-Patent Citations (5)
| Title |
|---|
| LICHENG PENG,等: "Cultivation of Freshwater Green Alga Neochloris oleoabundans in Non-Sterile Media Co-Inoculated with Protozoa", 《THE CANADIAN JOURNAL OF CHEMICAL ENGINEERING》 * |
| TAMOTSU KANAZAWA等: "MASS CULTURE OF UNICELLULAR ALGAE USING THE "OPEN CIRCULATION METHOD"", 《J. GEN. APPL. MICROBIOL.》 * |
| 郝智慧等 译: "《小动物毒理学 第3版》", 31 October 2014, 中国农业大学出版社 * |
| 郭健等: "单胞藻培养中原生动物的控制与清除", 《河北渔业》 * |
| 陈晓亚等: "《植物生理与分子生物学》", 30 June 2007, 高等教育出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115537339A (en) * | 2022-09-22 | 2022-12-30 | 天津长芦汉沽盐场有限责任公司 | A kind of method of salina outdoor breeding to reduce insect damage |
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