CN109354204A - The ecological floating island degeneration system that a kind of immobilization biological filler and plant-bacillus subtilis lotus root are closed - Google Patents
The ecological floating island degeneration system that a kind of immobilization biological filler and plant-bacillus subtilis lotus root are closed Download PDFInfo
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- CN109354204A CN109354204A CN201811490596.2A CN201811490596A CN109354204A CN 109354204 A CN109354204 A CN 109354204A CN 201811490596 A CN201811490596 A CN 201811490596A CN 109354204 A CN109354204 A CN 109354204A
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- biological filler
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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Abstract
The invention belongs to environmental technology field, a kind of immobilization biological filler is provided and ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed.The preparation of the immobilization biological filler includes: the culture of (1) bacillus subtilis microbial agent: (2) carbon fiber bar ecological grass is the preparation of the immobilization biological filler of carrier.The very small B. subtilis cell of volume can be fixed in flowing water body by the immobilization biological filler, guarantee the normal growth of bacillus subtilis, so that the function and effect of bacillus subtilis are unaffected.The ecological floating island degeneration system includes: that composite ecological floating island, planting aquatic plants on chinampa are arranged in (1) in enclosure area；(2) immobilization biological filler is hung around under chinampa and chinampa.The degeneration system can enhance the degradation of the elements such as nitrogen in eutrophication water, phosphorus, removal, have preferable ecological benefits and social benefit.
Description
Technical field
The invention belongs to environmental technology fields, specifically, being related to a kind of immobilization biological filler and plant-withered grass gemma
The ecological floating island degeneration system that bacillus lotus root is closed.
Background technique
Since modern times, the industrial and agricultural development in China is very rapid, and a large amount of nitrogen phosphorus substance enters in water body, causes water
The eutrophication of body is serious, and therefore, the improvement to eutrophic water body is a social problem anxious to be resolved.Eutrophication
The improvement of water body can be divided into external source control and endogenous control, and external source controls the input that can reduce nitrogen and phosphorus element, but in water body
Internal loading the problem of can making water eutrophication continue many decades.
Currently, including chemical method, physical method and bioanalysis for water eutrophication administering method.But chemical method and physics
Method is not only cumbersome, and cost is high, and the risk with secondary pollution.Common technology includes revegetation skill in bioanalysis
Art and microorganism remediation technology.Revegetation technology be mainly adjusted by water plant aquatic ecosystem substance circulation and
Energy flow controls the eutrophic extent of water body with this.But it in the serious area of water body eutrophication degree, is planted
It is reconstructed extremely difficult.Microorganism remediation technology is mainly to administer rich battalion in the way of can adsorbing, absorb by microorganism, degrading etc.
Feedingization water body.Microorganism remediation technology have many advantages, such as it is at low cost, high-efficient, easy to operate, without secondary pollution, thus by more next
More concerns.But since microbial cell is too small, it is not easy to be fixed in flowing water body, so that the function and effect of microorganism
It is affected, application range is also restrained.
Summary of the invention
For deficiency above-mentioned in the prior art, the first object of the present invention is the provision of a kind of immobilization biological and fills out
Material；The very small B. subtilis cell of volume can be fixed in flowing water body by the immobilization biological filler, and be protected
The normal growth of bacillus subtilis is demonstrate,proved, so that the function and effect of bacillus subtilis are unaffected, application range is wider.
For deficiency above-mentioned in the prior art, the second object of the present invention is the provision of a kind of immobilization biological filler
And the ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed；The degeneration system can enhance nitrogen in eutrophication water,
Degradation, the removal of the elements such as phosphorus, have preferable ecological benefits and social benefit.
In order to achieve the above object, the solution that the present invention uses is:
A kind of immobilization biological filler, is prepared using following steps: (1) culture of bacillus subtilis microbial agent: will
Strain is restored to room temperature state from preservation state, is inoculated into solid medium, and 37 DEG C of constant temperature, after cultivating 16h, picking shape is good
Good single colonie is inoculated into new solid medium, repeats this step 2 time, obtains activation bacterium；Activation bacterium is seeded to first
Culture solution, obtains seed liquor after cultivating 20h by 37 DEG C of constant temperature；It is packed into the second culture solution according to the 60-70% of fermenter volume, is adjusted
PH value is saved to 6.8-7.2, seed liquor is then accessed into fermentor according to the 2% of the second nutrient solution volume, controlled at 37 DEG C,
Air quantity is 2.5L/min, revolving speed 160-200r/min, dissolved oxygen 70-80%, and culture for 24 hours, obtains fermentation liquid；(2) charcoal element is fine
Dimension ecological grass is the preparation of the immobilization biological filler of carrier: fermentation liquid being fitted into 5L beaker, it is raw to be subsequently placed into carbon fiber bar
State grass 40-50 beam, sunlight irradiate 8-10h/ days, and the bacteria containing amount after 60-72h on carbon fiber bar ecological grass is 1.2 × 106-2.8×
107CFU/cm2, obtain immobilization biological filler.
A kind of ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed, comprising: (1) be arranged in enclosure area multiple
Close ecological floating island, planting aquatic plants on chinampa；(2) it is consolidating for carrier that carbon fiber bar ecological grass is hung around under chinampa and chinampa
Surely change biologic packing material.
The ecological floating island that a kind of immobilization biological filler provided by the invention and plant-bacillus subtilis lotus root are closed, which is degraded, is
The beneficial effect of system is:
(1) this kind of immobilization biological filler provided by the invention, is prepared using above-mentioned preparation method, can long-term sustained release
Bacillus subtilis can guarantee that B. subtilis cell is fixed in flowing water body for volume is very small, and guarantee
The normal growth of bacillus subtilis, so that the function and effect of bacillus subtilis are unaffected, application range is wider.
(2) the ecological floating island degeneration system that this kind of plant provided by the invention-bacillus subtilis lotus root is closed is based on aquatic plant
Object/microbial association recovery technique makes full use of the advantage of water plant and functional microorganism, constructs plant/bacillus subtilis
Bacterium joint reparation system selects the water plant and microorganism adaptable with it, will be microbial immobilized, thus to eutrophication
Water body carries out the reparation of ecology, and it is first to enhance nitrogen, phosphorus etc. in eutrophication water by the synergy of water plant and microorganism
It is dirty to be suitable for the polluted water body in-situs such as lake, river to reach to the most effective improvement of polluted-water for degradation, the removal of element
Dye is administered, plant/bacillus subtilis group of building can in water body preferable growth and breeding, there are preferable ecological benefits
And social benefit, and there is wide application space.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
The life that a kind of immobilization biological filler provided in an embodiment of the present invention and plant/bacillus subtilis lotus root are closed below
State chinampa degeneration system is specifically described.
A kind of immobilization biological filler, is prepared using following steps:
Prepare solid medium: tryptone 1.0%, yeast extract 0.5%, sodium chloride 1.0%, agar 1.5%, pH
Value is 7.0-7.2.
Prepare the first culture solution: molasses 1.0%, yeast powder 0.8%, NaCl1.0%, K2HPO40.25 ‰,
KH2PO40.25 ‰, MnSO4·H2O0.15 ‰, pH value 6.8-7.2.
Prepare the second culture solution: molasses 1.0%, yeast powder 0.8%, NaCl1.0%, K2HPO40.25 ‰,
KH2PO40.25 ‰, MnSO4·H2O0.2 ‰, pH value 6.8-7.2.
It should be noted that in the present embodiment, strain is selected from bacillus subtilis ATCC 35021.Ecological Carbon fibe grass
The specification of every 30 beam is 65cm × 40cm (24000/beam, 7 μm/root of diameter).
(1) culture of bacillus subtilis microbial agent: (1) culture of bacillus subtilis microbial agent: by strain from preservation state
It is restored to room temperature state, is inoculated into solid medium, 37 DEG C of constant temperature, after cultivating 16h, the good single colonie inoculation of picking shape
Into new solid medium, this step 2 time is repeated, obtains activation bacterium；Activation bacterium is seeded to the first culture solution, constant temperature 37
DEG C, seed liquor is obtained after cultivating 20h；It is packed into the second culture solution according to the 60-70% of fermenter volume, adjusts pH value to 6.8-
7.2, seed liquor is then accessed into fermentor, controlled at 37 DEG C, air quantity 2.5L/ according to the 2% of the second nutrient solution volume
Min, revolving speed 160-200r/min, dissolved oxygen 70-80%, culture for 24 hours, obtain fermentation liquid；
(2) carbon fiber bar ecological grass is the preparation of the immobilization biological filler of carrier: fermentation liquid being fitted into 5L beaker, is put
Enter carbon fiber bar ecological grass 40-50 beam, sunlight irradiates 8-10h/ days, and the bacteria containing amount after 60-72h on carbon fiber bar ecological grass is
1.2×106-2.8×107CFU/cm2, obtain immobilization biological filler.
A kind of ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed, comprising:
(1) composite ecological floating island, planting aquatic plants on chinampa are set in enclosure area；Water plant further includes
At least one of calamus, canna, reed, rice and Chinese celery.The selection of water plant is according to the dirt of staying water water quality
The difference of dye degree, using the water plant of various combination.
(2) around under chinampa and chinampa hang immobilization biological filler, biologic packing material using carbon fiber bar ecological grass as carrier,
Fixed bacillus subtilis.
In conclusion according to the width in lake and proposing the chinampa width setting ecological floating island set, planting aquatic plants.
Consolidating using carbon fiber bar ecological grass as carrier is hung under the upper part planting aquatic plants of ecological floating island, chinampa and around chinampa
Surely change filler, inorganic nutrient substances some in water body synthesis is conducive to the substance of plant absorption by the effect of microorganism, is reduced
Toxic action of the water pollutant to plant enhances absorption of the plant to inorganic salts such as N, P, while the secretion energy of plant rhizosphere
Carbon source and nutriment needed for enough providing growth for microorganism, maintain the stability of microorganism, are finally reached and administer water body
Purpose.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of immobilization biological filler is present embodiments provided, is prepared using following steps:
(1) culture of bacillus subtilis microbial agent: being restored to room temperature state from preservation state for strain, is inoculated into solid training
It supports in base, 37 DEG C of constant temperature, after cultivating 16h, chooses the good single colonie of shape and be inoculated into new solid medium, repeat this step
2 times, obtain activation bacterium；Activation bacterium is seeded to the first culture solution, 37 DEG C of constant temperature, obtains seed liquor after cultivating 20h；According to fermentation
The 70% of tank volume is packed into the second culture solution, adjusts pH value to 7.0, then by seed liquor according to the 2% of the second nutrient solution volume
Fermentor is accessed, controlled at 37 DEG C, air quantity 2.5L/min, revolving speed 180r/min, dissolved oxygen 80%, culture for 24 hours, is obtained
To fermentation liquid；
(2) carbon fiber bar ecological grass is the preparation of the immobilization biological filler of carrier: fermentation liquid being fitted into 5L beaker, is put
Enter 40 beam of carbon fiber bar ecological grass, sunlight irradiates 8h/ days, the bacteria containing amount after 60h on carbon fiber bar ecological grass for 5.5 ×
106CFU/cm2, obtain immobilization biological filler.
Present embodiments provide a kind of ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed, comprising: in enclosure
Composite ecological floating island is set in area, the water plants such as calamus, canna and reed are fixed in field planting basket, under chinampa and chinampa
Surrounding hangs immobilization biological filler.
Embodiment 2
A kind of immobilization biological filler is present embodiments provided, is prepared using following steps:
(1) culture of bacillus subtilis microbial agent: being restored to room temperature state from preservation state for strain, is inoculated into solid training
It supports in base, 37 DEG C of constant temperature, after cultivating 16h, the good single colonie of picking shape is inoculated into new solid medium, repeats this step
Rapid 2 times, obtain activation bacterium；Activation bacterium is seeded to the first culture solution, 37 DEG C of constant temperature, obtains seed liquor after cultivating 20h；According to hair
The 65% of fermentation tank volume is packed into the second culture solution, adjusts pH value to 7.2, then by seed liquor according to the second nutrient solution volume
2% access fermentor, controlled at 37 DEG C, air quantity 2.5L/min, revolving speed 200r/min, dissolved oxygen 70% is cultivated
For 24 hours, fermentation liquid is obtained；
(2) carbon fiber bar ecological grass is the preparation of the immobilization biological filler of carrier: fermentation liquid being fitted into 5L beaker, is put
Enter 45 beam of carbon fiber bar ecological grass, sunlight irradiates 9h/ days, the bacteria containing amount after 72h on carbon fiber bar ecological grass for 8.7 ×
106CFU/cm2, obtain immobilization biological filler.
Present embodiments provide a kind of ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed, comprising: in enclosure
Composite ecological floating island is set in area, the water plants such as calamus, rice and Chinese celery are fixed in field planting basket, under chinampa and chinampa week
Enclose suspension immobilization biological filler.
Embodiment 3
A kind of immobilization biological filler is present embodiments provided, is prepared using following steps:
(1) culture of bacillus subtilis microbial agent: being restored to room temperature state from preservation state for strain, is inoculated into solid training
It supports in base, 37 DEG C of constant temperature, after cultivating 16h, the good single colonie of picking shape is inoculated into new solid medium, repeats this step
Obtain activation bacterium for rapid 2 times；Activation bacterium is seeded to the first culture solution, 37 DEG C of constant temperature, obtains seed liquor after cultivating 20h；According to hair
The 60% of fermentation tank volume is packed into the second culture solution, adjusts pH value to 6.8, then by seed liquor according to the second nutrient solution volume
2% access fermentor, controlled at 37 DEG C, air quantity 2.5L/min, revolving speed 170r/min, dissolved oxygen 75% is cultivated
For 24 hours, fermentation liquid is obtained；
(2) carbon fiber bar ecological grass is the preparation of the immobilization biological filler of carrier: fermentation liquid being fitted into 5L beaker, is put
Enter 50 beam of carbon fiber bar ecological grass, sunlight irradiates 10h/ days, the bacteria containing amount after 70h on carbon fiber bar ecological grass for 1.9 ×
107CFU/cm2, obtain immobilization biological filler.
Present embodiments provide a kind of ecological floating island degeneration system that plant-bacillus subtilis lotus root is closed, comprising: in enclosure
Composite ecological floating island is set in area, the water plants such as rice, canna and reed are fixed in field planting basket, under chinampa and chinampa
Surrounding hangs immobilization biological filler.
Experimental example 1
Experimental method: the ecological floating island degeneration system that embodiment 1-3 is closed using plant-bacillus subtilis lotus root is detected respectively
The ammonia-nitrogen content in polluted-water before, total phosphorus content and COD content；And detection is withered using embodiment 1-3 offer plant-
The ecological floating island degeneration system that careless bacillus lotus root is closed carries out the ammonia-nitrogen content after degradation same time, and total phosphorus content and COD contain
Amount, and ammonia nitrogen removal frank is calculated, total tp removal rate and COD removal rate, the results are shown in Table 1:
Table 1
From the data in table 1, it can be seen that being dropped using the ecological floating island that plant provided in an embodiment of the present invention-bacillus subtilis lotus root is closed
After solution system carries out water body degradation, the harmful substance in polluted-water can be significantly removed, there is preferable ecological benefits and society
It can benefit.
In conclusion the ecology closed using immobilization biological filler provided by the invention and plant-bacillus subtilis lotus root
Chinampa degeneration system；The immobilization biological filler can long-term sustained release bacillus subtilis, can be by the very small withered grass bud of volume
Spore bacilli-cell is fixed in flowing water body, raw for plant while the normal growth for guaranteeing bacillus subtilis, acting on
It is long that nutriment is provided, toxic action of the polluted-water object to plant is reduced, absorption of the plant to inorganic salts such as N, P, tool are enhanced
There is wider array of application.The degeneration system can enhance the elements such as nitrogen in eutrophication water, phosphorus degradation, removal, have compared with
Good ecological benefits and social benefit.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of immobilization biological filler, it is characterised in that: be prepared using following steps:
(1) culture of bacillus subtilis microbial agent: strain is restored to room temperature state from preservation state, is inoculated into solid medium
In, 37 DEG C of constant temperature, after cultivating 16h, the good single colonie of picking shape is inoculated into the new solid medium, repeats this step
Rapid 2 times, obtain activation bacterium；The activation bacterium is seeded to the first culture solution, 37 DEG C of constant temperature, obtains seed liquor after cultivating 20h；It presses
It is packed into the second culture solution according to the 60-70% of fermenter volume, adjusts pH value to 6.8-7.2, then by the seed liquor according to institute
The 2% access fermentor of the second nutrient solution volume is stated, controlled at 37 DEG C, air quantity 2.5L/min, revolving speed 160-
200r/min, dissolved oxygen 70-80%, culture for 24 hours, obtain fermentation liquid；
(2) carbon fiber bar ecological grass is the preparation of the immobilization biological filler of carrier: the fermentation liquid being fitted into 5L beaker, is connect
Be put into carbon fiber bar ecological grass 40-50 beam, sunlight irradiates 8-10h/ days, containing on the carbon fiber bar ecological grass after 60-72h
Bacterium amount is 1.2 × 106-2.8×107CFU/cm2, obtain immobilization biological filler.
2. immobilization biological filler according to claim 1, it is characterised in that: in step (1), the solid medium
Condition includes: tryptone 1.0%, yeast extract 0.5%, sodium chloride 1.0%, agar 1.5%, pH value 7.0-7.2.
3. immobilization biological filler according to claim 1, it is characterised in that: in step (1), first culture solution
Condition includes: molasses 1.0%, yeast powder 0.8%, NaCl1.0%, K2HPO40.25 ‰, KH2PO40.25 ‰, MnSO4·
H2O0.15 ‰, pH value 6.8-7.2.
4. immobilization biological filler according to claim 1, it is characterised in that: in step (2), second culture solution
Condition includes: molasses 1.0%, yeast powder 0.8%, NaCl1.0%, K2HPO40.25 ‰, KH2PO40.25 ‰, MnSO4·
H2O0.2 ‰, pH value 6.8-7.2.
5. immobilization biological filler according to claim 1, it is characterised in that: the rule of every 30 beam of the ecology Carbon fibe grass
Lattice are 65cm × 40cm；24000/beam, 7 μm/root of diameter.
6. the ecological floating island degeneration system that a kind of plant-bacillus subtilis lotus root is closed, it is characterised in that: include:
(1) composite ecological floating island, planting aquatic plants on the chinampa are set in enclosure area；
(2) immobilization biological filler described in claim 1-5 any one is hung around under chinampa and chinampa.
7. the ecological floating island degeneration system that plant according to claim 6-bacillus subtilis lotus root is closed, it is characterised in that:
The water plant includes at least one of calamus, canna, reed, rice and Chinese celery.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116530449A (en) * | 2023-04-11 | 2023-08-04 | 南京市水产科学研究所 | Artificial shrimp island capable of being used for aquatic weed planting and use method thereof |
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Application publication date: 20190219 |