CN109342377A - A pH Fiber Optic Sensor System Based on Fluorescence Excitation - Google Patents
A pH Fiber Optic Sensor System Based on Fluorescence Excitation Download PDFInfo
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- CN109342377A CN109342377A CN201811043391.XA CN201811043391A CN109342377A CN 109342377 A CN109342377 A CN 109342377A CN 201811043391 A CN201811043391 A CN 201811043391A CN 109342377 A CN109342377 A CN 109342377A
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- 238000005253 cladding Methods 0.000 claims description 4
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 claims description 4
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
The present invention is to provide a kind of pH fiber optic sensor systems based on fluorescence excitation principle.It is characterized in that: it is made of probe tip 1, pH fibre-optical probe 2, optical fiber connector 3, lens 4, first light source 5, second light source 6, the first dichroic filter 9, the second dichroic filter 10, bandpass filter 11, photodetector 12, analog-digital converter 13, control and signal processing system 14 and driving circuit 15.The present invention can be used for the pH value real-time measurement of active mass in the pH value measurement and organism of atomic small sample, can be widely used for the fields such as medical diagnosis, botany, biotechnology and microbiology.
Description
(1) technical field
The present invention relates to a kind of pH fiber optic sensor systems based on fluorescence excitation principle, can be used for atomic small sample
PH value measurement and organism in active mass pH value real-time measurement, belong to technical field of optical fiber sensing.
(2) background technique
In medical diagnosis (such as tissue metabolism's research, neuro-physiology, cancer diagnostics), botany, biotechnology and micro-
In biology, the pH value detection of tiny sampler is had a very important significance.For example, in tumour, intracellular ph value
Measurement is most important to the reaction of various treatments for monitoring cancer progression and cancer cell；The measurement of pH value can be helped in muscle
It helps and is classified to trauma patient and treated, while whether can also be used to display diabetic's peripheral blood flow bad；?
In plant tissue, the enzyme of the adjustable bioactive compound metabolism for participating in control plant growth and development of local pH value difference
Activity.With medical diagnosis and the development of microenvironment investigative technique, in medical treatment, the various fields such as scientific research are available to research and development
Increasingly increase in the demand of measurement micro volume sample or the miniaturization pH sensor for patient's body detection.
Traditionally, the method that the test equipment of pH value is all based on electrochemistry, but in recent years, have benefited from selective high, easy
In micromation, without electromagnetic interference the features such as, optical sensor gradually develop be a kind of substitute technology.It describes in the prior art
PH detecting instrument based on optical sensor using multichannel (multifiber) carry out light propagation (such as: document Nguyen, T.H.,
et al,2014.“Fluorescence based fibre optic ph sensor for the ph 10–13range
suitable for corrosion monitoring in concrete structures.”Sensors and
Actuators B:Chemical,191,pp.498-507；Document Socorro, A.B., et al, 2012. " Tapered
single-mode optical fibre pH sensor based on lossy mode resonances generated
By a polymeric thin-film. " Sensors Journal, IEEE, 12 (8), pp.2598-2603. and document
Singh,S.and Gupta,B.D.,2012.“Fabrication and characterization of a highly
sensitive surface plasmon resonance based fibre optic pH sensor utilizing
high index layer and smart hydrogel.”Sensors and Actuators B:Chemical,173,
Pp.268-273. described), this, which makes them usually, has biggish sensing probe, and it reduce light collection efficiencies, while also limiting
Their practicabilities in small samples measurement are made.In published invention, Zhao Hui etc. disclosed " a kind of in 2012
Two-parameter fibre optical sensor for pH value and solution oxygenation measurement " (Chinese Patent Application No.: 201210277262.3), realizes
It is measured while the pH and two-parameter dissolved oxygen of liquid；Shi Bingxin etc. disclosed " a kind of optic fibre pH meter " in 2014, and (China is specially
Sharp application number: 201310571847.0), pH value measurement based on fibre optical sensor is realized；Position credit light etc. is open in 2015
A kind of " Intelligent optical fiber pH sensor " (Chinese Patent Application No.: 201510034149.6), is detected in system using multi-pass
PH value, and the compensation of the parameters such as temperature is increased in the signal processing.
The Problems Existing of pH optical sensor described in document and disclosure of the invention described above has: needing multi-pass
The fluorescence that the exciting light and light-sensitive layer that (multi fiber) emits light source are excited transmits.This leads to the fluorescence being excited
It has and greatly inputs incident and transmission each optical path, cause the fluorescence for reaching photodetector to be weakened, detection efficient
It reduces.In addition, the sensing head of existing design is larger or directly uses optical fiber as sensing probe, it is easy to break since optical fiber is more crisp
It is disconnected, for atomic small sample and the sampling of living tissue or patient's lesion region and pH value must be surveyed which has limited them
Amount.In addition, although part system joined the compensation of the parameters such as temperature, but they do not mend light source outgoing optical power
It repays and perhaps adjusts when fluorescence signal changes greatly (as because the loss of fluorescent dye or fluorescent bleach effect lead to fluorescence
The decaying of signal), photodetector can not may accurately read fluorescence signal, and the measurement that this can reduce total system is accurate
Property.
In order to solve problem above, the invention discloses a kind of pH fiber sensor measuring system based on fluorescence excitation principle
System can be used for the pH value real-time measurement of active mass in the pH value measurement and organism of atomic small sample.The present invention uses single
Channel carries out fluorescence excitation and phosphor collection to the light-sensitive layer at Fibre Optical Sensor end, is not necessarily to light splitting during phosphor collection,
Fluorescence reception efficiency greatly improved, to use minimum probe in sensor ends；The sensing probe diameter of the design
It is close to or less than 100 microns, is detected conducive to minimum sample, while sensing probe is among probe tip, makes it
It is easy to that living tissue or patient's lesion region are inserted into or are sampled to carry out pH value detection；In addition, system uses double light
Source carries out fluorescence excitation to photosensitive material, can compensate to external environmental variance；Simultaneously in detection process, system can be right
The light intensity of light source outgoing adjusts in real time, so that photodetector is in normal range of operation, avoids detector because returning to fluorescence
It is too strong and be saturated or excessively weak and can not detect because returning to fluorescence, enhance the dynamic range and stability of total system.
(3) summary of the invention
The purpose of the present invention is to provide a kind of pH value that can be used for active mass in atomic small sample and organism is real
When the fiber optic sensor system that measures.
The object of the present invention is achieved like this:
The pH fiber optic sensor system based on fluorescence excitation principle is by probe tip 1, pH optical fiber sensing probe 2, light
Fiber connector 3, lens 4, first light source 5, second light source 6, the first dichroic filter 9, the second dichroic filter 10, band logical
Optical filter 11, photodetector 12, analog-digital converter 13, control and signal processing system 14 and driving circuit 15 form,
The optical fiber 21 that wherein sensor ends of pH optical fiber sensing probe 2 are removed after jacket layer and coat by one section is constituted, in removal jacket layer and
The end face of optical fiber 21 after coat is covered with one layer of light-sensitive layer 22.The sensor ends of pH optical fiber sensing probe 2 in the system
It in probe tip 1, in measurement, can be inserted into sample container, the living tissue detected needed for also can be inserted into such as human body disease
Become in region or blood vessel.When system is run, in the case where controlling the control with signal processing system 14, driving circuit 15 makes first
Light source 5 be not overlapped mutually on 6 generation time coordinate of second light source but the identical pulsed light of intensity；The pulse that first light source 5 issues
Light is reflected into optical fiber connector 3 through the first dichroic filter 9 and is coupled into pH optical fiber sensing probe 2 by lens 4, to pH
The light-sensitive layer 22 of the sensor ends of optical fiber sensing probe 2 is excited, the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2
The fluorescence of generation passes through pH optical fiber sensing probe 2, optical fiber connector 3, lens 4, the first dichroic filter 9, the second dichroic
Optical filter 10 and bandpass filter 11 are incident to photodetector 12, and photodetector 12 turns the fluorescence signal detected
It is changed to electric signal, and is input in control and signal processing system 14 by analog-digital converter 13；What second light source 6 issued
Pulsed light is reflected through the second dichroic filter 10, enters optical fiber connector 3 through the first dichroic filter 9 and by lens 4
It is coupled into pH optical fiber sensing probe 2, the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2 is excited, pH optical fiber
The fluorescence that the light-sensitive layers 22 of the sensor ends of sensing probe 2 generates by pH optical fiber sensing probe 2, optical fiber connector 3, lens 4,
First dichroic filter 9, the second dichroic filter 10 and bandpass filter 11 are incident to photodetector 12, and photoelectricity is visited
It surveys device 12 and the fluorescence signal detected is converted into electric signal, and be input to control and signal by analog-digital converter 13
In processing system 14；Two fluorescence signals that control and signal processing system 14 excite first light source 5 and second light source 6 into
Row calculates and provides measurement result after handling.
Light source used can be one of LED light source, laser diode light source, SLD light source, ASE light source in system.
Photodetector used can be PIN photodiode in system, avalanche photodide, single-photon detector with
And any one in photomultiplier tube.
PH optical fiber sensing probe 2 in system can be that (fibre core 9~100 is micro- based on standard single mode/multimode silica fibre
Rice, direct 125 microns of covering) either smaller cladding diameter special silica fibre (such as GI50/80-29/165 type fiber,
Cladding diameter be 80 microns) optical fiber sensing probe.One end of pH optical fiber sensing probe 2 is fiber coupler interface, for connecing
Enter optical fiber connector 3；The other end is sensor ends, is the optical fiber (containing fibre core and covering) after one section of removal jacket layer and coat, covers
Diameter can be within 100 microns, for being packed into probe tip 1 after lid photosensitive material.The size of probe tip 1 can be greater than for internal diameter
100 microns of any syringe needle, the syringe needle including general international standard 32G (110 microns of internal diameter) to 14G (1.54 millimeters of internal diameter).
One layer of covering is photosensitive made of fluorescent dye and sol gel process on the sensor ends fiber end face of pH optical fiber sensing probe 2
Feel layer 22, fluorescence can be issued when being excited by the light of specific wavelength, the intensity of the fluorescence inspired can be with institute's sample
The variation of pH value and change, production method are as follows: the pH fluorescent dye of 50mg (bromocresol purple (BP) is used as pH fluorescent dye) with
Straight alcohol (EtOH) mixing.Silica precursor (tetraethyl orthosilicate (TEOS)), 0.1M aqueous hydrochloric acid solution and using EtOH as base
The pH fluorescent dye solution of plinth prepares silicon substrate colloidal sol under the molar ratio of 1:0.007:6.25.It is small that this colloidal sol is vigorously stirred 48
When.Fiber end face is immersed into prepared silicon substrate colloidal sol with the impregnating speed of 24 mm/mins to prepare sensing membrane.Fiber end face
It need to immerse once, impregnate 30 minutes, later solidify the optical fiber sensing probe prepared 24 hours in 70 DEG C of oven.
When system is run, first light source 5 and 6 controlled emissions of second light source go out that optical wavelength is different and the identical pulse of light intensity
Light, the two pulsed lights are not overlapped mutually on time coordinate, i.e., when first light source 5 shines, second light source 6 stops shining, on the contrary
?.The optical wavelength that first light source 5 issues is less than the light that second light source 6 issues.First dichroic filter 9 is by wavelength
It is issued greater than first light source 5, while (optical signal that i.e. second light source 6 issues will not for the optical wavelength including the sending of second light source 6
It is reflected by the first dichroic filter 9).Second dichroic filter 10 is the light issued greater than second light source 6 by wavelength
Wavelength.
Control and signal processing system 14 are sat by the control first light source 5 of driving circuit 15 and the sending time of second light source 6
Put on the identical pulsed light of light intensity not being overlapped mutually.Control simultaneously and signal processing system 14 pass through photodetector 12 and simulation
Digital quantizer 13 receives the return fluorescence excited by first light source 5 and second light source 6.In order to enable fluorescence detection normally into
Row, i.e. photodetector 12 will not be saturated since return fluorescence is too strong, will not can not be detected because return fluorescence is excessively weak,
The variation of the external environments such as temperature is compensated simultaneously, control and signal processing system 14 can be adjusted simultaneously by driving circuit 15
The output intensity of first light source 5 and second light source 6 makes first light source 5 that the fluorescence signal returned be excited to keep constant, passes through meter
Calculation first light source 5 and second light source 6 excite the pH value of radiometer calculating institute's sample of the fluorescence signal returned.
Compared to prior art, advantages of the present invention has: 1. carry out fluorescence using sensing membrane of the single channel to Fibre Optical Sensor end
Without light splitting during phosphor collection fluorescence reception efficiency greatly improved, so that in sensor ends in excitation and phosphor collection
It is possibly realized using minimum probe；2. sensing probe is only 15 microns of diameter, minimum sample can be detected；Spy is sensed simultaneously
Head makes it easier to carry out pH value detection to living tissue or patient's lesion region among probe tip；3. detection process
Fluorescence excitation is carried out to photosensitive material using double light sources, external environmental variance can be compensated, simultaneity factor is emitted light source
Light intensity adjust in real time so that photodetector is in normal range of operation, it is too strong and full because returning to fluorescence to avoid detector
And/or can not be detected because return fluorescence is excessively weak, enhance the dynamic range and stability of total system.
(4) Detailed description of the invention
Fig. 1 is the schematic diagram of the pH Optical Fiber Sensor Measurement System based on fluorescence excitation principle.System by probe tip 1,
PH fibre-optical probe 2, optical fiber connector 3, lens 4, first light source 5, second light source 6, the first dichroic filter the 9, the 2nd 2 to
Colo(u)r filter 10, bandpass filter 11, photodetector 12, analog-digital converter 13, control and 14 and of signal processing system
Driving circuit 15 forms.
Fig. 2 is the schematic diagram of the embodiment of the pH Optical Fiber Sensor Measurement System based on fluorescence excitation principle.This embodiment
It is the pH value measurement that the system be used to carry out patient's pathological tissues, system is by probe tip 1, pH optical fiber sensing probe
2, optical fiber connector 3, lens 4, first light source 5, second light source 6, the first dichroic filter 9, the second dichroic filter 10,
Bandpass filter 11, photodetector 12, analog-digital converter 13, control and signal processing system 14 and 15 groups of driving circuit
At.
Fig. 3 is the control schematic diagram that system emits first light source 5 and second light source 6 pulsed optical signals.In system
First light source 5 and 6 controlled emissions of second light source go out the pulsed light that wavelength is respectively 470nm and 480nm, the two pulsed light light intensity
It is identical, but be not overlapped mutually on time coordinate, i.e., when first light source 5 shines, second light source 6 stops shining, and vice versa,
And photodetector 12 receives corresponding excitation fluorescence signal, and exports to control and signal processing system 14 and carry out data
Storage.
Fig. 4 is shown under the excitation of different wavelengths of light and in different pH value samples, returns to fluorescence signal in wave
Light intensity (fluorescent intensity that photodetector is detected) within the scope of long 515nm ± 10nm.
Fig. 5 is shown after the light intensity of system call interception light source 1 and light source 2, and the fluorescent intensity for exciting 470nm keeps permanent
Periodically, the fluorescent exciting under the excitation of different wavelengths of light and within the scope of different pH value sample medium wavelength 515nm ± 10nm
By force.
What Fig. 6 was indicated is the wavelength that light source 2 (480nm) and light source 1 (470nm) are excited in different pH value samples
Fluorescent intensity ratio within the scope of 515nm ± 10nm.
(5) specific embodiment
Below with reference to specific embodiment, the present invention is further explained.
Fig. 2 gives the embodiment of the pH Optical Fiber Sensor Measurement System based on fluorescence excitation principle.This embodiment is institute
The pH value measurement that the system of stating be used to carry out patient's pathological tissues.System is by probe tip 1, pH optical fiber sensing probe 2, light
Fiber connector 3, lens 4, first light source 5, second light source 6, the first dichroic filter 9, the second dichroic filter 10, band logical
Optical filter 11, photodetector 12, analog-digital converter 13, control and signal processing system 14 and driving circuit 15 form,
The optical fiber 21 that wherein sensor ends of pH optical fiber sensing probe 2 are removed after jacket layer and coat by one section is constituted, in removal jacket layer and
The end face of optical fiber 21 after coat is covered with one layer of light-sensitive layer 22.When measuring, the probe tip 1 of system is inserted into patient
Pathological tissues are sampled, and the sample (liquid) in surveyed region reaches 2 sensor ends of pH optical fiber sensing probe by probe tip 1
Light-sensitive layer 22.When system is run, in the case where controlling the control with signal processing system 14, driving circuit 15 makes first light source 5
Be not overlapped mutually on 6 generation time coordinate of second light source but the identical pulsed light of intensity；The pulsed light that first light source 5 issues is through the
One dichroic filter 9 is reflected into optical fiber connector 3 and is coupled into pH optical fiber sensing probe 2 by lens 4, passes to pH optical fiber
The light-sensitive layer 22 of the sensor ends of sense probe 2 is excited, what the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2 generated
Fluorescence passes through pH optical fiber sensing probe 2, optical fiber connector 3, lens 4, the first dichroic filter 9, the second dichroic filter
10 and bandpass filter 11 be incident to photodetector 12, the fluorescence signal detected is converted to electricity by photodetector 12
Signal, and be input in control and signal processing system 14 by analog-digital converter 13；The pulsed light that second light source 6 issues
It reflects through the second dichroic filter 10, enter optical fiber connector 3 through the first dichroic filter 9 and is coupled by lens 4
Enter pH optical fiber sensing probe 2, the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2 is excited, pH Fibre Optical Sensor is visited
The fluorescence that the light-sensitive layer 22 of first 2 sensor ends generates passes through pH optical fiber sensing probe 2, optical fiber connector 3, lens the 4, the 1st
Photodetector 12, photodetector 12 are incident to colo(u)r filter 9, the second dichroic filter 10 and bandpass filter 11
The fluorescence signal detected is converted into electric signal, and is input to control and signal processing system by analog-digital converter 13
In system 14；Two fluorescence signals that control and signal processing system 14 excite first light source 5 and second light source 6 calculate
With measurement result is provided after processing.
First light source 5 and 6 controlled emissions of second light source in system go out the pulsed light that wavelength is respectively 470nm and 480nm,
The two pulsed light light intensity are identical, but are not overlapped mutually on time coordinate, i.e., when first light source 5 shines, second light source 6 stops
It only shines, vice versa (as shown in Figure 3), and photodetector 12 receives corresponding excitation fluorescence signal, and exports to control
System and signal processing system 14 carry out data storage.First dichroic filter 9, the second dichroic filter 10 are long logical optical filtering
Piece can be respectively > 475nm and > 485nm by wavelength；Bandpass filter 11 is 515nm ± 10nm by wavelength.
Control and signal processing system 14 are sat by the control first light source 5 of driving circuit 15 and the sending time of second light source 6
Put on the identical pulsed light of light intensity not being overlapped mutually.Control simultaneously and signal processing system 14 pass through photodetector 12 and simulation
Digital quantizer 13 receives the return fluorescence excited by first light source 5 and second light source 6.In order to enable fluorescence detection normally into
Row, i.e. photodetector 12 will not be saturated since return fluorescence is too strong, will not can not be detected because return fluorescence is excessively weak,
Control and signal processing system 14 can control the output intensity of first light source 5 and second light source 6 by driving circuit 15, make first
Light source 5 excites the fluorescence signal returned to keep constant, and excites the glimmering of return by calculating first light source 5 and second light source 6
The radiometer of optical signal calculates the pH value of institute's sample.
Fig. 4 is shown under the excitation of light sources with different wavelengths and in different pH value samples, returns to fluorescence signal and exists
Light intensity (fluorescent intensity that photodetector is detected) within the scope of wavelength 515nm ± 10nm.Double light used in this example
The wavelength in source is respectively 470nm and 480nm, in order to make photodetector be in normal range of operation, is avoided because returning to fluorescence
It is too strong and be saturated or excessively weak and can not detect because returning to fluorescence, while the variation of the external environments such as temperature is compensated, system
The light intensity that light source 1 and light source 2 can be adjusted simultaneously, the fluorescent intensity for exciting 470nm are kept constant, and Fig. 5 is shown in this feelings
Fluorescence excitation under condition, under the excitation of different wavelengths of light and within the scope of different pH value sample medium wavelength 515nm ± 10nm
Light intensity.Fluorescent intensity within the scope of wavelength 515nm ± 10nm that system pH value calculated is then excited by light source 2 and light source 1
Ratio, the visible Fig. 6 of experimental result.In the stability test carried out to system, the experimental results showed that, have benefited from being emitted light source
The real-time adjustment of light intensity and algorithm with the ratio between pair light source activation fluorescent intensities pH value calculated, whole pH detection system
Stability has obtained significant increase.
Claims (4)
1. a kind of pH fiber optic sensor system based on fluorescence excitation principle.It is characterized in that: it is passed by probe tip 1, pH optical fiber
Sense probe 2, optical fiber connector 3, lens 4, first light source 5, second light source 6, the first dichroic filter 9, the filter of the second dichroic
Mating plate 10, bandpass filter 11, photodetector 12, analog-digital converter 13, control and signal processing system 14 and driving
Circuit 15 forms, and wherein the sensor ends of pH optical fiber sensing probe 2 are made of the optical fiber 21 after one section of removal jacket layer and coat,
The end face of optical fiber 21 after removal jacket layer and coat is covered with one layer of light-sensitive layer 22.PH optical fiber sensing probe in the system
2 sensor ends are located in probe tip 1, in measurement, can be inserted into sample container, the living tissue detected needed for also can be inserted into
In human lesion region or blood vessel.When system is run, in the case where controlling the control with signal processing system 14, driving circuit 15
So that first light source 5 be not overlapped mutually on 6 generation time coordinate of second light source but the identical pulsed light of intensity；First light source 5 is sent out
Pulsed light out is reflected into optical fiber connector 3 and is coupled into pH Fibre Optical Sensor by lens 4 and visits through the first dichroic filter 9
First 2, the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2 is excited, the light of the sensor ends of pH optical fiber sensing probe 2
The fluorescence that sensitive layer 22 generates passes through pH optical fiber sensing probe 2, optical fiber connector 3, lens 4, the first dichroic filter 9, the
Two dichroic filters 10 and bandpass filter 11 are incident to photodetector 12, and photodetector 12 is glimmering by what is detected
Optical signal is converted to electric signal, and is input in control and signal processing system 14 by analog-digital converter 13；Second light
The pulsed light that source 6 issues is reflected through the second dichroic filter 10, enters optical fiber connector 3 through the first dichroic filter 9
And it is coupled into pH optical fiber sensing probe 2 by lens 4, the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2 is swashed
Hair, the fluorescence that the light-sensitive layer 22 of the sensor ends of pH optical fiber sensing probe 2 generates are connected by pH optical fiber sensing probe 2, optical fiber
Device 3, lens 4, the first dichroic filter 9, the second dichroic filter 10 and bandpass filter 11 are incident to photodetection
Device 12, the fluorescence signal detected is converted to electric signal by photodetector 12, and is inputted by analog-digital converter 13
Into control and signal processing system 14；Control two excited with signal processing system 14 to first light source 5 and second light source 6
A fluorescence signal is calculated and provides measurement result after being handled.
2. according to claim 1 based on 1 He of probe tip employed in glimmering light activated pH fiber optic sensor system
PH optical fiber sensing probe 2, it is characterized in that: pH optical fiber sensing probe 2 can be based on standard single mode/multimode silica fibre (fibre core 9
~100 microns, direct 125 microns of covering) either smaller cladding diameter special silica fibre (such as GI 50/80-29/165
Type fiber, cladding diameter be 80 microns) optical fiber sensing probe.One end of pH optical fiber sensing probe 2 connects for fiber coupler
Mouthful, it is used for incoming fiber optic connector 3；The other end is sensor ends, be after one section of removal jacket layer and coat optical fiber (containing fibre core and
Covering), diameter can be within 100 microns, for being packed into probe tip 1 after covering photosensitive material.The size of probe tip 1 can be
Internal diameter is greater than 100 microns of any syringe needle, including general international standard 32G (110 microns of internal diameter) to 14G (1.54 millimeters of internal diameter)
Syringe needle.One layer is covered on the sensor ends fiber end face of pH optical fiber sensing probe 2 by fluorescent dye and sol gel process system
At light-sensitive layer 22, by specific wavelength light excite when can issue fluorescence, the intensity of the fluorescence inspired can be with institute
The variation of the pH value of sample and change, production method are as follows: (bromocresol purple (BP) is used as pH fluorescence for the pH fluorescent dye of 50mg
Dyestuff) it is mixed with straight alcohol (EtOH).Silica precursor (tetraethyl orthosilicate (TEOS)), 0.1M aqueous hydrochloric acid solution and with
PH fluorescent dye solution based on EtOH prepares silicon substrate colloidal sol under the molar ratio of 1:0.007:6.25.This colloidal sol is acutely stirred
It mixes 48 hours.Fiber end face is immersed into prepared silicon substrate colloidal sol with the impregnating speed of 24 mm/mins to prepare sensing membrane.Light
Fine end face need to be immersed once, impregnate 30 minutes, it is small that the optical fiber sensing probe prepared is solidified to 24 in 70 DEG C of oven later
When.
3. first light source 5 according to claim 1, second light source 6, the first dichroic filter 9, the second dichroic filter
Piece 10.It is characterized in that: first light source 5 and 6 controlled emissions of second light source go out that optical wavelength is different and the identical pulsed light of light intensity, this two
A pulsed light is not overlapped mutually on time coordinate, i.e., when first light source 5 shines, second light source 6 stops shining, and vice versa.The
The optical wavelength that one light source 5 issues is less than the light that second light source 6 issues.First dichroic filter 9, the second dichroic filter 10
For long pass filter, the first dichroic filter 9 is to issue greater than first light source 5 by wavelength, while including the second light
The optical wavelength that source 6 issues (optical signal that i.e. second light source 6 issues will not be reflected by the first dichroic filter 9).Two or two to
Colo(u)r filter 10 is the optical wavelength issued greater than second light source 6 by wavelength.
4. control according to claim 1 and signal processing system 14, driving circuit 15.It is characterized in that: control and signal
Processing system 14 controls first light source 5 by driving circuit 15 and second light source 6 issues the light intensity not being overlapped mutually on time coordinate
Identical pulsed light.Control simultaneously and signal processing system 14 by photodetector 12 and analog-digital converter 13 receive by
The return fluorescence that first light source 5 and second light source 6 are excited.In order to enable fluorescence detection is normally carried out, i.e. photodetector 12
It will not be saturated, will not can not be detected because return fluorescence is excessively weak, while external to temperature etc. since return fluorescence is too strong
Environmental change compensates, and control and signal processing system 14 can adjust simultaneously first light source 5 and second by driving circuit 15
The output intensity of light source 6 makes first light source 5 that the fluorescence signal returned be excited to keep constant, by calculating first light source 5 and the
Two light sources 6 excite the pH value of radiometer calculating institute's sample of the fluorescence signal returned.
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