CN109315397B - A kind of L-carvone aerosol - Google Patents

Abstract

The invention belongs to the technical field of research and development of agricultural fungicides, in particular to a L-carvone aerosol. A L-carvone aerosol is composed of the following raw materials by weight percentage: L-carvone 0.1-2%, emulsifier 0.5-1%, penetrant 0.1-0.5%, and the solvent is supplemented to 100%. The L-carvone aerosol of the invention is a fungicide formulation suitable for preventing and controlling indoor plant diseases, which makes the prevention and control of indoor plant diseases simpler, more convenient and easier to operate.

Description

A kind of L-carvone aerosol

technical field

The invention belongs to the technical field of research and development of agricultural fungicides, in particular to a L-carvone aerosol.

Background technique

At present, the cultivation of indoor plants is very common in my country. Indoor plants not only play the role of beautifying the space in form, but also can adjust the indoor small environment, absorb harmful gases, meet people's spiritual needs, and play a role in edifying temperament. However, indoor plants lack suitable pesticide formulations for pest control. Most of the pesticides used are products registered on horticultural plants in the field, and they have bad odor when used indoors. In addition, the preparations are large in weight and high in active ingredients, and are weighed when used. It is troublesome, the concentration of preparation is not easy to grasp, one bag of preparation is often not used up at a time, and the storage is inconvenient, which brings difficulties to the maintenance of indoor plants.

L-carvone is the main component of the volatile oil extracted from Mentha spicata Linn., a perennial herb of the Lamiaceae family. It has been proved by experiments that spearmint volatile oil and its main component, L-carvone, have bacteriostatic activity against a variety of plant pathogenic fungi, and have the value of being developed as fungicides.

Studies have shown that a variety of plant volatile oils have inhibitory effects on phytopathogenic fungi, but most of these studies remain in the stage of indoor in vitro assays, and have not been processed into preparations for use in production. Aerosols have the advantages of being easy to carry, easy to use and store, and are widely used in the prevention and control of indoor hygiene pests. However, this formulation has not been developed for the control of plant diseases.

In order to solve the problems existing in the prior art, the present invention selects L-carvone as the research object, and develops a fungicide formulation suitable for indoor plants, so that the prevention and control of indoor plant diseases is simple, convenient and easy to operate.

The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.

SUMMARY OF THE INVENTION

In order to solve the problems that indoor plant diseases are common and the existing fungicide preparations are troublesome to dispense, use and store, the present invention provides a L-carvone aerosol and a preparation method thereof.

The technical scheme provided by the present invention is:

Use of L-carvone as an agricultural fungicide.

A L-carvone aerosol is composed of the following components by weight percentage: 0.1-2% of L-carvone, 0.5-1% of emulsifier, 0.1-0.5% of penetrant, and the solvent is supplemented to 100%.

Preferably, the emulsifier is at least one of lauric acid monoglyceride, calcium dodecylbenzenesulfonate, Tween series and Span series.

Preferably, the emulsifier is Tween 80.

Preferably, the solvent is at least one of water, ethanol, n-butanol, tert-butanol and n-propanol.

Preferably, the solvent is a mixed solution with a mass percentage of water and ethanol of 2:3.

Preferably, the penetrant is at least one of aliphatic alcohol polyoxyethylene ether and organosilicon series auxiliaries.

Preferably, the penetrant is aliphatic alcohol polyoxyethylene ether.

The present invention also provides the preparation method of the L-carvone aerosol. The amount of solvent is diluted to 100% and continues to emulsify to obtain L-carvone aerosol.

The medicinal liquid of the L-carvone aerosol obtained by the present invention is sealed and packaged, and the manual compressed air is used as the propellant to replace the propellants such as freon, propane, butane gas commonly used in the current aerosol, and the environmental impact is reduced. pollution.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The active ingredient of the L-carvone aerosol of the present invention, L-carvone, is derived from plants, has little influence on the indoor environment, and can increase indoor aroma.

(2) The L-carvone aerosol of the present invention is convenient to use, and can be directly sprayed after the plant is sick, and the dosage is small, and operations such as adding water and weighing are not required.

(3) The packaging of the L-carvone aerosol of the present invention can avoid the direct contact between the medicinal liquid and the outside world, is safe for users, and is convenient for storage.

(4) The preparation method of the L-carvone aerosol of the present invention is simple and suitable for industrial production.

Detailed ways

The specific embodiments of the present invention will be described in detail below with reference to the examples, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

Unless expressly stated otherwise, throughout the specification and claims, the term "comprising" or its conjugations such as "comprising" or "comprising" and the like will be understood to include the stated elements or components, and Other elements or other components are not excluded.

Example 1 :

0.5 g of L-carvone, 0.8 g of Tween 80, 0.5 g of fatty alcohol polyoxyethylene ether, and 38.2 g of water were weighed into the reaction device, stirred until completely emulsified, and then 60 g of ethanol was added and stirred evenly. The medicinal liquid is put into the aerosol tank to obtain 0.5% L-carvone aerosol.

Example 2 :

0.1 g of L-carvone, 0.6 g of lauric acid monoglyceride, 0.4 g of silicone penetrant SE-90, and 38.9 g of water were weighed into the reaction device, stirred until completely emulsified, and then 60 g of ethanol was added and stirred evenly. The medicinal liquid is loaded into the aerosol tank to obtain 0.1% L-carvone aerosol.

Example 3 : In vitro antibacterial effect of L-carvone

Test drug: L-carvone

Tested strains: Colletotrichum orchidearum, Botrytis cinerea.

Determination method: growth rate method of toxic medium

Under sterile conditions, dilute L-carvone with 6% Tween 80 solution into a series of concentration gradients, add it to a certain amount of PDA medium at about 50°C, and then pour into a culture medium with a diameter of 9 cm after fully shaking and mixing. In the dish, each concentration was 3 replicates, and the control medium was PDA supplemented with 6% Tween 80. For inoculation and culture, use a hole punch with a diameter of 5mm to take an appropriate amount of bacterial cake and insert it into the toxic medium and the control medium, with the mycelium side down, receive 1 bacterial cake per plate, and place it in an incubator for cultivation. After the mycelium in the culture dish of the control group had grown sufficiently, the colony diameters of the treatment group and the control group were measured by the cross method, and the percentage inhibition of the growth rate of the bacteria by each treatment concentration was calculated.

Bacteriostatic rate=(control colony growth diameter-treatment colony growth diameter)/control colony growth diameter

×100%.

Table 1 Bacteriostatic effect of L-carvone

It can be seen from Table 1 that the L-carvone of the present invention has a good inhibitory effect on orchid anthracnose and peony botrytis.

Example 4 : Pot test of L-carvone aerosol

Test agents: 0.1% L-carvone aerosol, 0.5% L-carvone aerosol, 50% carbendazim wettable powder.

Determination method: Both pathogens were inoculated by spore suspension spray, and the spore suspension (1×10 ⁶ ) was sprayed on the leaves of the plants to inoculate the bacteria. Protective effect: inoculate bacteria 24 hours after L-carvone aerosol treatment, and inoculate bacteria 24 hours before aerosol treatment for therapeutic effect. Clear water was used as blank control, and carbendazim 700 mg/L treatment was used as standard drug control. Each treatment was repeated 4 times. After inoculation, they were cultured in a humidified greenhouse, and the control was investigated after the disease was fully developed.

Control effect (%)=(control disease finger-treated disease finger)/control disease finger×100.

Table 2 Control effect of L-carvone on orchid anthracnose and peony botrytis

As can be seen from Table 2, two treatments of L-carvone aerosols of the present invention have good preventive effects on orchid anthracnose bacterium and rose black spot bacterium, and their preventive effects are all higher than the commonly used chemical bactericide carbendazim, and The protective effect is better than the therapeutic effect.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise form disclosed, and obviously many changes and modifications are possible in light of the above teachings. The exemplary embodiments were chosen and described for the purpose of explaining certain principles of the invention and their practical applications, to thereby enable others skilled in the art to make and utilize various exemplary embodiments and various different aspects of the invention. Choose and change. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims (9)

1. The application of the levo-carvone as an agricultural bactericide is characterized in that the agricultural bactericide is used for preventing and treating plant diseases caused by plant pathogenic bacteria, and the plant pathogenic bacteria are orchid colletotrichum gloeosporioides and peony botrytis cinerea.

2. A carvone aerosol containing the carvone of claim 1, which is characterized by comprising the following raw materials in percentage by weight: 0.1-2% of levo carvone, 0.5-1% of emulsifier, 0.1-0.5% of penetrant and solvent, and the balance is made up to 100%.

3. The carvone aerosol as claimed in claim 2, wherein the emulsifier is at least one of lauric acid monoglyceride, calcium dodecylbenzene sulfonate, tween series and span series.

4. The aerosol formulation of levocarvone according to claim 2, wherein the emulsifier is tween 80.

5. The carvone aerosol as claimed in claim 2, wherein the solvent is at least one of water, ethanol, n-butanol, t-butanol and n-propanol.

6. The carvone aerosol as claimed in claim 2, wherein the solvent is water and ethanol in a mass percentage of 2: 3, and (b).

7. The levocarvone aerosol as claimed in claim 2, wherein the penetrating agent is at least one of fatty alcohol-polyoxyethylene ether and silicone series auxiliary agents.

8. The levocarvone aerosol formulation according to claim 2, wherein the penetrant is fatty alcohol-polyoxyethylene ether.

9. The method for preparing the levocarvone aerosol as claimed in claim 2, which comprises the following steps: adding the levocarvone, the emulsifier, the penetrant and the solvent into a reaction device, uniformly stirring until complete emulsification is achieved, adding sufficient solvent to dilute until 100%, and continuing emulsification to obtain the levocarvone aerosol.