CN109315356A - A hatching and feeding device suitable for whiteflies or parasitic wasps and a method for using the same - Google Patents
A hatching and feeding device suitable for whiteflies or parasitic wasps and a method for using the same Download PDFInfo
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- CN109315356A CN109315356A CN201811100453.6A CN201811100453A CN109315356A CN 109315356 A CN109315356 A CN 109315356A CN 201811100453 A CN201811100453 A CN 201811100453A CN 109315356 A CN109315356 A CN 109315356A
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- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 claims description 7
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/30—Rearing or breeding invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
本发明涉及一种适用于粉虱类或寄生蜂类昆虫孵化、饲喂装置及方法，该装置包括饲喂层和储液层，饲喂层叠放设置在储液层之上配合连接；所述的饲喂层和储液层均为上底开口的中空圆柱体；在饲喂层的顶部设置有直径略大于其侧壁的卡叠圈；在饲喂层的侧壁上设置有若干开口；在饲喂层的底部具有若干孔洞，在孔洞中穿插设有棉花柱，棉花柱外部包裹有纱布，在孔洞上部覆盖有保水棉花，在保水棉花上面盖设有滤纸。本发明不仅使用方便、制作简单、能对粉虱和寄生蜂类的昆虫进行不同的孵化和饲养，有效的延长饲喂添加饲料的间隔周期，而且做到提高两种昆虫羽化率和存活率的目的，使饲养扩繁变得快速而简单，大大提高了工作效率。
The invention relates to a hatching and feeding device and method suitable for whiteflies or parasitic wasps. The device comprises a feeding layer and a liquid storage layer, and the feeding layer is arranged on the liquid storage layer for cooperation and connection; the The feeding layer and the liquid storage layer are hollow cylinders with an upper bottom opening; the top of the feeding layer is provided with a clamping ring with a diameter slightly larger than its side wall; a number of openings are provided on the side wall of the feeding layer; There are several holes at the bottom of the feeding layer, and cotton columns are inserted in the holes. The cotton column is wrapped with gauze, the upper part of the holes is covered with water-retaining cotton, and the water-retaining cotton is covered with filter paper. The invention is not only convenient to use, simple to manufacture, capable of different hatching and rearing of whiteflies and parasitic wasps, effectively prolonging the interval between feeding and adding feed, but also improving the emergence rate and survival rate of the two insects. The purpose is to make breeding and multiplying become fast and simple, and greatly improve the work efficiency.
Description
Technical field
The invention belongs to technical field of insect feeding, and in particular to a kind of multi-functional aleyrodid and parasitic wasp class insect were raised
The device and application method technical field fed in journey.
Background technique
Aleyrodid class pest is a class pest biggish to murrain in China's agricultural production process, and China also
There are invasive species --- and Bemisia tabaci Bemisia tabaci (Gennnadius) includes 30 or more hidden kind of species complex.
While aleyrodid makees beyond the region of objective existence with serious harm property to cotton, soybean and vegetables etc., moreover it is possible to the growth to plants such as landscape flowers
It causes to seriously affect.Several direct modes for endangering plant are piercing and sucking water to such worm mostly, and energy while piercing and sucking
Plant virus is enough propagated, report is more than 100 kinds by the plant virus type that aleyrodid is propagated at present, can cause to have no harvest when serious, lead
Cause huge economic loss.Aleyrodid has become the important pests on the crops such as Cotton in China, tobacco and vegetables, to agricultural production structure
At seriously threatening.Now worldwide positive injection huge profit with hostile its in parasitic wasp class day of aleyrodid carries out biological control,
Prevention and treatment have it is environmentally friendly, it is harmless to animal, not will cause the problems in chemical prevention.
The integrated control pest attention by more and more people participated in using biological control, wherein aleyrodid class pest
Prevention and treatment in it is also at the startup to ascent stage using the strategy of the natural enemy insects prevention and treatment such as parasitic wasp, need to be solved there are also problems
Certainly.So being acquired raising to aleyrodid in physical signs research and the research of anti-medicine in the biological characteristics to aleyrodid pest
It is indispensable link；Meanwhile it being also required to raise parasitic wasp in the prevention and control research of the hostile aleyrodid in parasitic wasp class day.?
In technology at this stage aleyrodid class insect from often needed in field acquisition on blade ovum and pupa carry out moisturizing culture, culture side
More the most commonly used is wrap up cotton using the direct blades shank of culture dish or be directly encased inside cotton in culture dish to be protected for method
It is wet, or be combined with and carry out moisturizing preservation raising using agar；But be directly wrapped in the method for blade grip operate it is more numerous
It is trivial, and the cotton of shank is easy to dehydration and air-dries, and adds moisture contained therein after water also less；And if using in culture dish
When padding cotton, if cotton is thicker, excessive waste both will cause, and the water that surface is difficult to obtain lower part keeps wet；It utilizes
Agar carries out blade in the operation of moisturizing, and the configuration of agar will take a substantial amount of time and energy, and agar is easier
For fungi bacterium provides living environment and it is mouldy to cause, it is at high cost more to waste；Furthermore from field acquisition to host plant leaf
There are many moulds for band mostly for piece, and blade or the matrix of culture mouldy pollution blade are be easy to cause during preservation, causes to cultivate
Failure, during the cultivation process for problem mouldy in the device of moisturizing also to further solve.And in the raising of parasitic wasp
The hydromel method for breeding one of more cheap as main and cost, raising is to utilize lesser culture in existing technology
Ware closely fills in cotton ball, and then addition hydromel carries out the raising of parasitic wasp in cotton, but bee is had in the method
The cotton of mulse is easy to air-dry, and will lead to parasitic wasp death if replacement not in time, so often to carry out matching for hydromel
It sets and adds, when addition is also easy to that hydromel benefit culture dish is caused to pollute, and operates relatively complicated and because frequent
Operation, needs to be replaced frequently the cotton in culture dish.Therefore design it is a kind of can significantly improve labor efficiency, reduce unnecessary money
Source waste, and it is features simple structure, easy to operate, the hatching rate and eclosion rate of aleyrodid class insect can be significantly improved, parasitic wasp is improved and raises
The device and method of feeding efficiency in supporting, are the striving directions for solving existing situation, for these problems, we to aleyrodid and
The device and application method of parasitic wasp class insect feeding have carried out invention research.
Summary of the invention
The present invention provides one kind and incubates suitable for aleyrodid class or parasitic wasp class insect precisely in order to the defect that solves the above problems
Change, feeding device and its application method, the device is not only easy to use, production is simple, can be to the insect of aleyrodid and parasitic wasp class
Different hatching and raising are carried out, the effective gap periods for extending feeding addition feed, and accomplish to improve two kinds of insect plumages
The purpose of rate and survival rate makes raising expansion is numerous to become quick and simple, greatly improves work efficiency.
The present invention adopts the following technical scheme that realization.
One kind being suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, and the present invention includes feeding layer and reserve liquid layer,
Feeding stacking puts setting and is cooperatively connected on reserve liquid layer；The feeding layer and reserve liquid layer are the hollow circuit cylinder of upper bottom opening
Body；The card stack circle that diameter is slightly larger than its side wall is provided at the top of feeding layer；Several open is provided on the side wall of feeding layer
Mouthful；There are several holes in the bottom of feeding layer, intert in hole and be equipped with cotton column, be enclosed with gauze outside cotton column,
Hole top is covered with water conservation cotton, is covered with filter paper on water conservation cotton.
Further it is that the hole of feeding layer bottom of the present invention is provided with 4-6, is uniformly arranged on feeding layer bottom
By ectocentral position；The setting of multiple holes, which can guarantee, can radiate after the water diffusion that cotton column draws on entire feeding layer
Cotton everywhere, and this position be arranged so that moisture by center around uniformly diffusion, and be not easy feeding layer in margin location
It sets and to form ponding insect is caused to be drowned by a large amount of ponding.
It is further to be provided on the inner ring of feeding layer lower part outer ring and reserve liquid layer top of the present invention mutually matched
Turn silk, the setting for turning silk will feed layer and reserve liquid layer and be fitted close, and liquid will not be overflowed from junction in reserve liquid layer, will not be from
Junction evaporation；And after separating reserve liquid layer and feeding layer, reserve liquid layer is relatively closed, can prevent liquid therein from polluting.
It is further that filling opening is provided on reserve liquid layer side wall of the present invention, filling opening opening is provided with dust-proof
Sealing cover；Dust seal cover lid forms sealing to filling opening after closing filling opening.The setting of filling opening guarantees adding into reserve liquid layer
Can be more fast and simple when liquid feeding body, it does not need to remove reserve liquid layer is separated into addition with feeding layer, can directly open sealing cover
It is added, dustproof cover can effectively prevent when not using filling opening usually that insect will not be thence into, while prevent dust
It is entered in reserve liquid layer Deng from filling opening.
It is further that device of the present invention is that from top to bottom sequentially combination superposition is set for multiple feeding layers and reserve liquid layer
It sets, is superposed height as 2-5 layers of combination；Dust seal cover on the filling opening of each reserve liquid layer side wall is in sealing cover conjunction
State.Multiple devices can be overlapped mutually, can be more effectively using worm space is supported, and the opening that layer side wall is fed after stacking can guarantee
Insect still can enter feeding on cotton, or therefrom fly out；Each reserve liquid layer has filling opening can be more easily after stacking
Dustproof cover is opened to be added and do not have to remove.
It is further that the material of feeding layer and reserve liquid layer of the present invention is transparent plastic or glass；Transparent plastic or
Glass on the one hand not will cause insect and cause concentration class excessive the taxis of other colors, on the other hand can more aspect
The liquid height observed in reserve liquid layer, and can completely see feeding layer in the hatching situation of aleyrodid and taking for parasitic wasp
Eat situation；Cotton column and water conservation cotton are absorbent cotton, and absorbent cotton is that scientific research material is often used in laboratory, are easy to obtain, and degreasing
Cotton has good diversion effect and moistening effect, achievees the purpose that raise two kinds of insects；Filter paper is circular filter paper, filter paper cost compared with
It is low, it is also more convenient when can newly change, and replace after first use.
It is further that device thickness of the present invention is 0.1cm；Reserve liquid layer base diameter is 5-10cm, high 4cm；It raises
Feed layer bottom and side wall diameter 5-10cm, high 5cm；Feeding layer sidewall opening is rectangle, and length and width 2cm, sidewall opening is away from feeding
Layer bottom 2-3cm；Described hole diameter is 0.5-1cm, and the water conservation cotton covered thereon is retained cotton and filter paper with a thickness of 1cm
Diameter is 5-10cm；Card stack loop diameter is greater than feeding layer side wall 0.1-0.5cm.There have with the above ideal specification to be following good
Place, one device thickness and is of moderate size, and does not have biggish quality, can satisfy be placed in the insect cage of two kinds of insects completely
It uses；Secondly the thickness of cotton, which not only can guarantee, guides to feeding layer for the liquid runs down cotton column of reserve liquid layer, but also can guarantee will not be compared with
The liquid of fast evaporation feeding layer；Device using when can one hand hold, it is easy to operate.
Further it is that the cotton column lower end that outside of the present invention is enclosed with gauze is tied up using filament, makes cotton column
Whole is in inverted cone-shaped, this structure can to make clear water because of suction that speed will not be too fast when moving upwards, and can be continuously feeding
The water conservation cotton of layer provides continuous moisture, is also able to maintain cotton post shapes after the tired bundle of filament and fixes.
A kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device, the dress using the present invention
Set the method to aleyrodid class insect with ovum and pupa blade moisturizing, comprising the following steps:
Step 1: laboratory is taken back after field collects the blade with aleyrodid ovum and pupa, carries out raising indoors numerous
It grows；It will be dried after device wiping using 75% alcohol, covering tightly the dust seal cover on the filling opening on reserve liquid layer side wall makes to store up
The filling opening of liquid layer seals, and the clear water of 2/3 volume is then added in reserve liquid layer, is encased inside in the feeding layer with cotton column
The absorbent cotton of 1cm thickness, and upper filter paper is covered on the cotton, it is then that feeding layer and reservoir bed is close using silk cooperation is turned, at this time
Cotton column end portion is immersed in clear water；It is sprayed on cotton and filter paper with the small watering can equipped with mould inhibitor wet；
Step 2: selection have aleyrodid ovum and pupa blade, according on blade pupa density carry out moisturizing culture: a) ovum or
When the density of pupa is smaller, with blade punch or scissors by 1-2cm around blade2Blade remove, vacuum side of blade is covered upward
On wet filter paper, multiple band ovum blades removed are placed in same device in the case where not stacking；B) ovum or pupa density
Higher, when punch or scissors cannot be used to remove, blade grip is inserted between cotton and side wall from device edge, and blade is carried on the back
It overlays on wet filter paper and cultivates up；
Step 3: be put into again after the blade handled well and device are placed into lesser insect cage in growth cabinet into
Row culture, growth cabinet condition setting are as follows: 26 ± 1 DEG C of temperature, humidity 70% ± 5%, photoperiod 14:10, illumination
18000lx；Add within every 5-7 days that a clear water is hatched to ovum or pupa is sprouted wings in tank body layer, after when addition opens dustproof cover
Clear water is extracted with plastic needle cylinder to be injected into reserve liquid layer from filling opening；When device is set as multiple layer combination, reserve liquid layer is successively opened
Filling opening on side wall is added；Aleyrodid nymph, the adult hatched is directly tested or is transferred on new host plant
Raising.
The mass fraction of mould inhibitor of the present invention configuration are as follows: 1%-5% benzoic acid, 10%-15% sorbic acid,
The mould inhibitor that clear water dissolution is configured to 1L is added in 0.1%-0.5% calcium propionate, 0.05%-0.1% ethyl-para-hydroxybenzoate
Liquid.
A kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device, the dress using the present invention
Set the method to the feeding of aleyrodid class parasitic wasp, comprising the following steps:
Step 1: distilled water flushing will be used after device wiping in the present invention using 75% alcohol, covers tightly reserve liquid layer bottom side
The dust seal cover on filling opening on wall seals the filling opening of reserve liquid layer bottom；Standard weighs honey on balance, is added
Warm water is configured to the hydromel that honey quality score is 10% ± 2%, is placed into room temperature after mixing evenly；Extraction configures
Hydromel be added in present apparatus reserve liquid layer 2/3 height, the degreasing of 1cm thickness is encased inside in the feeding layer with cotton column
Cotton；Then by feeding layer and reservoir bed, using turning, silk cooperation is close, and cotton column end portion is immersed in hydromel at this time；, use
Small watering can equipped with hydromel is sprayed to cotton in cotton and moistens；
Step 2: the feeding device equipped with hydromel is placed into parasitic wasp insect cage every 10-15 days in tank body layer
It is middle to add primary configured hydromel, it by hydromel by honey quality score is 10% ± 2% configuration when addition, it is to be cooled to arrive
Hydromel is extracted with plastic needle cylinder after room temperature, is injected into reserve liquid layer after dustproof cover is opened from filling opening, liquid still adds to
2/3 position of reserve liquid layer volume；
Step 3: the degreasing cotton in the primary feeding layer of replacement in every 20-30 days during the feeding process, it will be old de- when replacement
Rouge cotton removes, and new absorbent cotton is encased inside in feeding layer, and is sprayed and moistened with the hydromel watering can for filling identical mass fraction, according to
Actual conditions select the 30-40 days cleaning present apparatus and carry out the replacement of cotton.
The invention has the benefit that the present invention is a kind of to be suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device
And method, using the above structure and method for breeding, the reserve liquid layer can directly store liquid feed wherein in the present invention
Or water, and import has been issued on the side wall of this layer, it solves and needs to beat whole device when adding clear water and hydromel
The troublesome operation opened；Whole device has been divided into reserve liquid layer and feeding layer, is effectively separated two different functional areas,
It not will cause mutual pollution, and there is longer service life, can be fitted close after device combination, guarantee will not arbitrarily be fallen；
The inter-species for wherein feeding layer has aperture, and be inserted with cotton column, and two parts accomplished are closely connected, in the water of reserve liquid layer
It can be from, into the cotton of feeding layer, being easy to cause cotton very fast when solving in traditional feeding individually with culture dish on cotton column
The problem of moistening effect is not achieved is air-dried, is not needed through moisturizing of spraying water on frequentation blade.In the moisturizing of aleyrodid class insect ovum and pupa
It is covered with filter paper on the cotton of feeding layer in method, can be conducive to replace next time on the basis of keeping blade wet, subtract
Few contaminated generation of cotton, compared to culture is carried out using agar, the operation is more convenient；Parasitic wasp is raised using this device
When feeding, cotton can carry out the utilization of long period, and the addition time of hydromel expands, and cotton service life extends, symbol
It closes and saves and sustainable thought, reduction waste；Use can be overlapped mutually between several devices by card stack circle, can effectively be utilized
Space in insect cage, and it is able to cooperate the use of a variety of insect cage specifications, the side wall for feeding layer has opening, ensure that in phase
Parasitic wasp still without hindrance feeding hydromel when mutually superposition uses.
The of the invention method that aleyrodid class insect is hatched with ovum and pupa blade moisturizing and to the feeding of aleyrodid class parasitic wasp
Method uses distilled water flushing when in use after first being wiped device with 75% alcohol, can guarantee and carry out disinfection place to device
Reason, reducing in device especially reserve liquid layer has microbial reproduction, and can be reduced the microorganism in feeding layer under cotton, reaches more
Long service life because of pollution effect without using；In the host plant method for moisturizing of aleyrodid class insect, using fresh-keeping mould proof
Agent and using equipped with mould inhibitor small watering can be sprayed on cotton and filter paper it is wet, and it is indirect be configured in liquid have it is following
Benefit: one is that mould inhibitor component selected in the present invention is to use in food and safe to the human body, in moisturizing process
In can elder brother on the people and blade that can also prevent the mould having on blade from breeding and using on the cotton fresh-keeping to blade
Worm is harmless；Secondly for using watering can sprinkling, wet can solve is directly loadable into reserve liquid layer in later period liquid feeding on the cotton and extremely
It needs to configure mould inhibitor every time, and sprays on the cotton since its solution is originally configured mass fraction mould inhibitor, because
The loss of mould inhibitor main component is smaller, as the evaporation of part steam is smaller since the clear water of bottom supplements its composition transfer, and
And clear water is directly added into when needing in reserve liquid layer liquid feeding.It is no more than the 2/3 of reserve liquid layer volume in reserve liquid layer liquid feeding, both
Guarantee that liquid is not overflowed from the filling opening of side wall, also guarantees biggish to shake from feeding layer when needing mobile and overflow；It is first
Cotton is squirted with small watering can when the secondary layer cotton using feeding, service efficiency is improved, can directly carry out moisturizing or raising,
Thereafter the humidity of water conservation cotton is provided because bottom cotton column energy continuous drainage just can reach lasting in raising, to raise elder brother
Worm, the mould on blade that less contact such as field is taken back to the sensibility of mould inhibitor and during the feeding process due to parasitic wasp,
Therefore it does not need to be added using mould inhibitor；When to hatching with aleyrodid class insect ovum or pupa, according to it on blade
Density it is selected punching, shearing or completeblade save mode, space can be utilized to greatest extent, and not will cause ovum
Or the damage of pupa；In the hatching breeding of aleyrodid class insect ovum or pupa, add a clear water moisturizing can complete its to hatching when
Blade moisture preserving time is placed in climate box and maintains metastable temperature, guarantees that it hatches as soon as possible；In raising parasitic wasp class
Insect when, the card stack circle on device can provide the place for stopping rest for parasitic wasp, and the hydromel fed is once adding
It can be used after adding 10-15 days, need to add within 3 days when compared to traditional parasitic wasp feeding and once significantly reduce the negative of raising insect
Load, experimenter can also extract free time out and carry out other experiment matters, liberate more labours.
Operation of the present invention is simple, can effectively raise to two class insects, and flexibility is high, saves manual labor, device
Cost of manufacture is cheap, can guarantee when studying the prevention and treatment problem of China aleyrodid class pest and when raising to parasitic wasp a large amount of
Indoor population can be promoted the use for experiment in each R&D institution and parasitic wasp Breeding base etc..
The present invention is further explained with reference to the accompanying drawings and detailed description.
Detailed description of the invention
Fig. 1 is feeding layer cotton filtering paper structure schematic diagram of the invention.
Fig. 2 is feeding schematic diagram of a layer structure of the invention.
Fig. 3 is liquid storage schematic diagram of a layer structure of the invention.
Fig. 4 is the device of the invention combination diagram.
Figure label are as follows: feeding layer (1), water conservation cotton (1.1), filter paper (1.2), reserve liquid layer (2), card stack circle (3), side wall
Opening (4), cotton column (6), turns silk (7), filling opening (8), dust seal cover (9) at hole (5).
Specific embodiment
In order to make those skilled in the art, methods of this invention will be better understood, combined with specific embodiments below to this hair
It is bright to be described in further detail.
Fig. 1, Fig. 2, Fig. 3 are seen, shown in Fig. 4.
One kind being suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, and the present invention includes feeding layer 1 and reserve liquid layer
2, feeding layer 1 stacks setting and is cooperatively connected on reserve liquid layer 2；The feeding layer 1 and reserve liquid layer 2 are in upper bottom opening
Hollow cylinder；The top of feeding layer 1 is provided with the card stack circle 3 that diameter is slightly larger than its side wall；It is arranged on the side wall of feeding layer 1
There are several openings 4；There are several holes 5 in the bottom of feeding layer 1, intert in hole and be equipped with cotton column 6, outside cotton column 6
It is enclosed with gauze, water conservation cotton 1.1 is covered on 5 top of hole, is covered with filter paper 1.2 on water conservation cotton 1.1.
Further it is that the hole 5 of feeding 1 bottom of layer of the present invention is provided with 4-6, is uniformly arranged on feeding layer 1
Ectocentral position is leaned in bottom.
It is further to be provided with mutual cooperation on 1 lower part outer ring of feeding layer of the present invention and 2 top inner ring of reserve liquid layer
Turn silk 7.
It is further that filling opening 8 is provided on 2 side wall of reserve liquid layer of the present invention, 8 opening of filling opening is provided with anti-
Dirt sealing cover 9；The lid of dust seal cover 9 forms sealing to filling opening 8 after closing filling opening 8.
It is further that device of the present invention is that from top to bottom sequentially combination superposition is set for multiple feeding layers 1 and reserve liquid layer 2
It sets, is superposed height as 2-5 layers of combination；Dust seal cover 9 on the filling opening 8 of each 2 side wall of reserve liquid layer is in sealing
Lid conjunction state.
It is further that the material of feeding layer 1 and reserve liquid layer 2 of the present invention is transparent plastic or glass；Cotton column and
Water conservation cotton is absorbent cotton；Filter paper is circular filter paper.
It is further that device thickness of the present invention is 0.1cm；2 base diameter of reserve liquid layer is 5-10cm, high 4cm；It raises
Feed layer 1 bottom and side wall diameter 5-10cm, high 5cm；Feeding 1 sidewall opening of layer is rectangle, and length and width 2cm, sidewall opening is away from feeding
Feed 1 bottom 2-3cm of layer；5 diameter of described hole is 0.5-1cm, and the water conservation cotton 1.1 covered thereon is retained cotton with a thickness of 1cm
1.1 and 1.2 diameter of filter paper be 5-10cm；3 diameter of card stack circle is greater than feeding 1 side wall 0.1-0.5cm of layer.
Further it is that 6 lower end of cotton column that outside of the present invention is enclosed with gauze is tied up using filament, makes cotton column
6 it is whole be in inverted cone-shaped, this structure can to make clear water because of suction that speed will not be too fast when moving upwards, and can be continuously feeding
The water conservation cotton of layer provides continuous moisture, is also able to maintain cotton post shapes after the tired bundle of filament and fixes.
A kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device, the dress using the present invention
Set the method to aleyrodid class insect with ovum and pupa blade moisturizing, comprising the following steps:
Step 1: laboratory is taken back after field collects the blade with aleyrodid ovum and pupa, carries out raising indoors numerous
It grows；It will be dried after device wiping using 75% alcohol, the dust seal cover 9 covered tightly on the filling opening 8 on 2 side wall of reserve liquid layer makes
The filling opening of reserve liquid layer 2 seals, and the clear water of 2/3 volume is then added in reserve liquid layer, pads in the feeding layer with cotton column
Enter the absorbent cotton of 1cm thickness, and covers upper filter paper on the cotton, it is then that feeding layer and reservoir bed is close using silk cooperation is turned, this
Shi Mianhua column end portion is immersed in clear water；It is sprayed to and is moistened i.e. on cotton and filter paper with the small watering can equipped with mould inhibitor
It can；
Step 2: selection have aleyrodid ovum and pupa blade, according on blade pupa density carry out moisturizing culture: a) ovum or
When the density of pupa is smaller, with blade punch or scissors by 1-2cm around blade2Blade remove, vacuum side of blade is covered upward
On wet filter paper, multiple band ovum blades removed are placed in same device in the case where not stacking；B) ovum or pupa density
Higher, when punch or scissors cannot be used to remove, blade grip is inserted between cotton and side wall from device edge, and blade is carried on the back
It overlays on wet filter paper and cultivates up；
Step 3: be put into again after the blade handled well and device are placed into lesser insect cage in growth cabinet into
Row culture, growth cabinet condition setting are as follows: 26 ± 1 DEG C of temperature, humidity 70% ± 5%, photoperiod 14:10, illumination
18000lx；Add within every 5-7 days that a clear water is hatched to ovum or pupa is sprouted wings in tank body layer, after when addition opens dustproof cover
Clear water is extracted with plastic needle cylinder to be injected into reserve liquid layer from filling opening；When device is set as multiple layer combination, reserve liquid layer is successively opened
Filling opening on side wall is added；Aleyrodid nymph, the adult hatched is directly tested or is transferred on new host plant
Raising.
The mass fraction of the mould inhibitor configuration are as follows: 5% benzoic acid, 10% sorbic acid, 0.2% calcium propionate, 0.1% pair
The mould inhibitor liquid that clear water dissolution is configured to 1L is added in nipagin A.
A kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device, the dress using the present invention
The method to the feeding of aleyrodid class parasitic wasp is set, is included the following steps
Step 1: distilled water flushing will be used after device wiping in the present invention using 75% alcohol, covers tightly 2 bottom side of reserve liquid layer
The dust seal cover 9 on filling opening 8 on wall seals the filling opening of 2 bottom of reserve liquid layer；Standard weighs honey on balance, adds
Enter warm water and be configured to the hydromel that honey quality score is 10% ± 2%, is placed into room temperature after mixing evenly；Extract configuration
Good hydromel is added in present apparatus reserve liquid layer 2/3 height, and the degreasing of 1cm thickness is encased inside in the feeding layer with cotton column
Cotton；Then by feeding layer and reservoir bed, using turning, silk cooperation is close, and cotton column end portion is immersed in hydromel at this time；, use
Small watering can equipped with hydromel is sprayed to cotton in cotton and moistens；
Step 2: the feeding device equipped with hydromel is placed into parasitic wasp insect cage every 10-15 days in tank body layer
It is middle to add primary configured hydromel, it by hydromel by honey quality score is 10% ± 2% configuration when addition, it is to be cooled to arrive
Hydromel is extracted with plastic needle cylinder after room temperature, is injected into reserve liquid layer after dustproof cover is opened from filling opening, liquid still adds to
2/3 position of reserve liquid layer volume；
Step 3: the degreasing cotton in the primary feeding layer of replacement in every 20-30 days during the feeding process, it will be old de- when replacement
Rouge cotton removes, and new absorbent cotton is encased inside in feeding layer, and is sprayed and moistened with the hydromel watering can for filling identical mass fraction, according to
Actual conditions select the 30-40 days cleaning present apparatus and carry out the replacement of cotton.
Specific embodiment is described in detail.
Specific operation process
The present invention is furture elucidated combined with specific embodiments below, and present embodiment detailed process is with skill of the present invention
Implemented under premised on art scheme, it should be understood that these modes are only used for illustrating model of the invention rather than that limitation is of the invention
It encloses.
(1) apparatus structure
Summary: device includes feeding layer and reserve liquid layer in the present invention, and feeding stacking, which puts to be arranged on reserve liquid layer to cooperate, to be connected
It connects；The feeding layer and reserve liquid layer are the hollow cylinder of upper bottom opening；It is bigger that diameter is provided at the top of feeding layer
In the card stack circle of its side wall；Several openings are provided on the side wall of feeding layer；In the bottom of feeding layer there are 4-6 uniformly to set
It sets in feeding layer bottom by ectocentral position hole, interts in hole and be equipped with cotton column, be enclosed with gauze outside cotton column,
It is covered with water conservation cotton on hole top, is covered with filter paper on water conservation cotton；It is provided with filling opening on reserve liquid layer side wall, is added
Liquid mouth opening is provided with dust seal cover；The material of invention described device is transparent plastic or glass.
Device specification
Device thickness of the present invention is 0.1cm；Reserve liquid layer base diameter is 8cm, high 4cm；Feed layer bottom and side
Wall diameter 8cm, high 5cm；Feeding layer sidewall opening is rectangle, and length and width 2cm, sidewall opening is away from feeding layer (1) bottom 2cm；Institute
Stating hole diameter is 1cm, and the water conservation cotton covered thereon is retained cotton and filter paper diameter is 8cm with a thickness of 1cm；Card stack circle is straight
Diameter is greater than feeding layer side wall 0.5cm.
(2) selected insect source and experiment
Aleyrodid class insect ovum and pupa culture experiment: test has selected kind to be implanted with 3 kinds of tomato, sunflower, kidney bean crop leaves
The aleyrodid ovum and pupa of on piece are tested, by aleyrodid pupa collected on three of the above plant using of the invention when experiment
Device carries out moisturizing culture, and in addition acquisition direct filled cotton with culture dish and carried out in fact using the methods of agar moisturizing with traditional
It tests.
Experiment one: being investigated by 3 pieces of samples of every kind of plant of Systematic selection, is obtained in tomato, sunflower, kidney bean 3
The density of Bemisia tabaci pupa on kind plant leaf blade；Experiment two: using 3 kinds of pupa cultural methods respectively on collected 3 kinds of plants
Bemisia tabaci pupa cultivated, when acquisition, which climbs over plant leaf blade discovery, has aleyrodid pupa to take blade, the blade placement of acquisition
In acquisition tank, each 50 blades of 3 kinds of plants of random acquisition, and carry out the punching selection of pupa after taking back laboratory and shear choosing
Device is taken and be ready to, compares the time of three kinds of methods to obtain labor efficiency；Experiment three: dress of the invention is respectively adopted
It sets, traditional direct moisturizing culture experiment for filling cotton with culture dish and using 3 kinds of methods of agar moisturizing with pupa blade, culture is straight
Sprout wings out to pupa and other pupas dry out death when statistics eclosion rate and plus water moisturizing number.
The mass fraction of the mould inhibitor configuration used in this method are as follows: 5% benzoic acid, 10% sorbic acid, 0.2% propionic acid
The mould inhibitor liquid that clear water dissolution is configured to 1L is added in calcium, 0.1% ethyl-para-hydroxybenzoate.
Aleyrodid parasitic wasp class insect raising experiment: band is collected by the leaf with black aleyrodid pupa of parasitic wasp parasitism in field
Piece is saved using upper aleyrodid class pupa store method, the eclosion rate of parasitic wasp is counted after expanding numerous parasitic wasp out, and using this
The method for breeding and conventional culture dish of invention add the mode of cotton to be raised, and two methods add the number of hydromel in one month
And cotton replaces number.
Aleyrodid pupa and parasitic bee pupa density on (3) 3 kinds of different plants
Aleyrodid pupa and parasitic bee pupa density ratio on the different plants of 13 kinds of table compared with
Note: numerical value is average value ± standard error in figure.Same column difference lowercase indicates different plot powder on same plant
Lice and parasitic bee pupa density difference in size are significant (P < 0.05, Duncan ' s duncan's new multiple range method)；A, B, C are represented not same in table
Ground.
Table 1 aleyrodid pupa on kindred plant differently in population density have differences, wherein aleyrodid pupa kind
The pupa density of the A sample ground of eggplant is higher than the population density of B, C sample ground, but the not significant (F of difference2,15=0.68, P >
0.05), the density of the C sample ground on sunflower is higher than the pupa density of A, B sample ground, the not significant (F of difference2,15=0.98,
P > 0.05), and the pupa density of C sample ground is significantly higher than the pupa density (F of B sample ground on kidney bean2,15=2.68, P >
0.05)；With the aleyrodid pupa density highest of sunflower C sample ground on 3 kinds of plants, reach 21.17/leaf, tomato B sample ground
The minimum 5.50/leaf of population density.3 kinds of plants 3 samples on the ground parasitic bee pupa density without significant difference (tomato:
F2,15=1.01, P > 0.05；Sunflower: F2,15=0.05, P > 0.05；Kidney bean: F2,15=1.33, P > 0.05)；Wherein 3
On kind plant leaf blade, the parasitic bee pupa density with sunflower B sample ground is up to 10.00/leaf, the parasitism of tomato B sample ground
The minimum 0.83/leaf of bee pupa density.
(4) 3 kinds of different culture apparatus preparing experiment efficiency compare
23 kinds of table different culture apparatus preparing experiment efficiency compare
Table 2, which obtains, uses three kinds of different devices progress moisturizing culture aleyrodids and having in preparing experiment for parasitic bee pupa larger
Difference, wherein it is shorter using the time that traditional culture dish cotton culture needs to prepare, but need during the cultivation process repeatedly
Add water to squirt carry out moisturizing, and effect is still not satisfactory, although and use agar culture in period do not have to repeatedly plus water,
It is to need a large amount of time to be configured when configuring agar, influences conventional efficient；And in experiment standard when using apparatus of the present invention
The standby time needed is shorter, and in the process because there is reserve liquid layer much constantly to provide moisture, add water moisturizing number also compared with
It is few, it is opposite to have higher conventional efficient.
(5) 3 kinds of different cultural methods compare to the culture of aleyrodid pupa and to by parasitic bee pupa eclosion rate
The different cultural methods of table 3 compare to the culture of aleyrodid pupa and to by parasitic bee pupa eclosion rate
Table 3 obtains the rear emergence to the two for using 3 kinds of different cultural methods to carry out moisturizing culture aleyrodids and parasitic bee pupa
There are Different Effects for rate, in the pupa culture to aleyrodid, use present invention culture, 3 kinds of culture of culture dish cotton, agar culture sides
The aleyrodid pupa eclosion rate average value that method carries out on 3 kinds of plant leaf blades of culture is respectively 46.12%, 29.20%, 45.03%, wherein
Eclosion rate using more common culture dish cotton culture is minimum, and uses the eclosion rate difference of other two methods
Less, the method that the method that the present invention uses wants a little higher than agar culture；3 kinds of methods carry out posting on 3 kinds of plant leaf blades of culture
Raw bee pupa eclosion rate average value is respectively 53.36%, 36.20%, 51.59%, consistent with aleyrodid pupa cultivation results, common to train
It is still minimum for supporting the eclosion rate of ware cotton culture.Cultural method of the invention can effectively improve the eclosion rate of two kinds of insect pupas.
Two methods add the number of hydromel and cotton replacement number to compare in (6) one months
4 two methods of table add the number of hydromel and cotton replacement number to compare
Table 4 obtains the experiment for using 2 kinds of different method for breeding to raise parasitic wasp and adds water and replacement cotton number not
Together, it number of water of two kinds of feeding methods in climate box and is intended to changing cotton number less than time raised under environment indoors
Number；In the comparative experiments of two methods feeding, traditional feeding side will be less than by carrying out feeding using feeding method of the invention
In method plus water and replacement number, i.e., method of the invention raising when can be reduced feeding when the operating time, to improve effect
Rate.
By testing above and result can obtain, the method for the invention that aleyrodid class insect is hatched with ovum and pupa blade moisturizing and
Add a clear water moisturizing can be complete in the hatching breeding of aleyrodid class insect ovum or pupa the method for aleyrodid class parasitic wasp feeding
At it to blade moisture preserving time when hatching, it is placed in climate box and maintains metastable temperature, guarantee that it hatches as soon as possible；
When raising the insect of parasitic wasp class, and the hydromel fed can be used 10-15 days after primary addition, compared to tradition
Parasitic wasp feeding when need to add within 3 days the burden for once significantly reducing raising insect, experimenter can also extract free time out
Other experiment matters are carried out, more labours are liberated.It can be seen that the invention method prepares from the early-stage preparations of several method
Time is just shorter, and operation is also relatively simple, and does not have to often addition water in experiment later, because having reserve liquid layer structure
It provides；In the culture experiment to two kinds of insect pupas, although eclosion rate is only slightly taller than agar, efficiency is higher, and at
This is lower, and can obtain higher eclosion rate in the method for conventional culture dish cotton；Being even more in the experiment of parasitic wasp can be compared with
Good guarantee does not need addition hydromel during being used continuously for a long time.The device of the invention and method have behaviour in contrast
Make it is simple, raising effectively can be carried out to two class insects and expand numerous, suitable environment is relatively broad, and can cooperate a variety of insect cages,
Insectary and insect raising are expanded numerous greenhouse etc. and are used, and flexibility is high, saves manual labor, the features such as device cost of manufacture is cheap
Aleyrodid or parasitic wasp insects hatch, the device of feeding, method and result are described in detail above, still
As long as slightly being adjusted using method and structure of the invention, such as device size size, liquid feeding type and the side for saving raising pupa
Method etc. can carry out raising hatching to shaped like such insects such as trypetid, drosophila, butterfly classes.
Above-described is only specific embodiments of the present invention, and the common sense such as well known specific structure and characteristic exist in scheme
This is not described excessively.It should be pointed out that for those skilled in the art, without departing from the structure of the invention,
Several modifications and improvements can also be made, these also should be considered as protection scope of the present invention, these all will not influence the present invention
The effect and patent practicability of implementation.The scope of protection required by this application should be based on the content of the claims, explanation
The records such as the specific embodiment in book can be used for explaining the content of claim.
Claims (10)
1. one kind is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, which is characterized in that the device includes feeding layer
(1) it stacks setting with reserve liquid layer (2), feeding layer (1) and is cooperatively connected on reserve liquid layer (2)；The feeding layer (1) and liquid storage
Layer (2) is the hollow cylinder of upper bottom opening；The card stack circle that diameter is slightly larger than its side wall is provided at the top of feeding layer (1)
(3)；Several openings (4) are provided on the side wall of feeding layer (1)；There are several holes (5) in the bottom of feeding layer (1),
Intert in hole and be equipped with cotton column (6), be enclosed with gauze outside cotton column (6), is covered with water conservation cotton on hole (5) top
(1.1), filter paper (1.2) are covered on water conservation cotton (1.1).
2. one kind according to claim 1 is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, feature exist
In the hole (5) of described feeding layer (1) bottom is provided with 4-6, is uniformly arranged on feeding layer (1) bottom by ectocentral
Position；Mutually matched turn of silk (7) is provided on feeding layer (1) the lower part outer ring and reserve liquid layer (2) top inner ring.
3. one kind according to claim 1 is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, feature exist
In being provided with filling opening (8) on the reserve liquid layer (2) side wall, filling opening (8) opening is provided with dust seal cover (9)；It is anti-
Dirt sealing cover (9) lid forms sealing to filling opening (8) after closing filling opening (8).
4. one kind according to claim 1 is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, feature exist
In the device is that from top to bottom sequentially combination is superposed for multiple feeding layers (1) and reserve liquid layer (2), is superposed height
It is combined for 2-5 layers；The dust seal cover (9) on filling opening (8) on each reserve liquid layer (2) side wall is in sealing cover and closes shape
State.
5. one kind according to claim 1 is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, feature exist
In the material of the feeding layer (1) and reserve liquid layer (2) is transparent plastic or glass；Cotton column and water conservation cotton are absorbent cotton；
Filter paper is circular filter paper.
6. one kind according to claim 1 is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, feature exist
In cotton column (6) lower end that outside is enclosed with gauze is tied up using filament, makes cotton column (6) integrally in inverted cone-shaped.
7. one kind according to claim 1 is suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device, feature exist
In the device thickness is 0.1cm；Reserve liquid layer (2) base diameter is 5-10cm, high 4cm；Feed layer (1) bottom and side wall
Diameter 5-10cm, high 5cm；Feeding layer (1) sidewall opening is rectangle, and length and width 2cm, sidewall opening is away from feeding layer (1) bottom 2-
3cm；Described hole (5) diameter is 0.5-1cm, the water conservation cotton (1.1) covered thereon with a thickness of 1cm, be retained cotton (1.1) and
Filter paper (1.2) diameter is 5-10cm；Card stack circle (3) diameter is greater than feeding layer (1) side wall 0.1-0.5cm.
8. using a kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device described in claim 1-7,
It is characterized in that, method of the device to aleyrodid class insect with ovum and pupa blade moisturizing, comprising the following steps:
Step 1: laboratory is taken back after field collects the blade with aleyrodid ovum and pupa, carries out captive breeding indoors；Make
It will be dried after device wiping with 75% alcohol, cover tightly the dust seal cover (9) on the filling opening (8) on reserve liquid layer (2) side wall
The filling opening of reserve liquid layer (2) is sealed, the clear water of 2/3 volume is then added in reserve liquid layer, in the feeding layer for having cotton column
In be encased inside the absorbent cotton of 1cm thickness, and cover upper filter paper on the cotton, then will feeding layer and reservoir bed it is tight using silk cooperation is turned
Close, cotton column end portion is immersed in clear water at this time；It is sprayed on cotton and filter paper with the small watering can equipped with mould inhibitor wet
Profit；
Step 2: selection has the blade of aleyrodid ovum and pupa, carry out moisturizing culture according to the density of pupa on blade: a) ovum or pupa
When density is smaller, with blade punch or scissors by 1-2cm around blade2Blade remove, vacuum side of blade is overlayed on upward wet
On the filter paper of profit, multiple band ovum blades removed are placed in same device in the case where not stacking；B) ovum or pupa density are higher,
When punch or scissors cannot be used to remove, blade grip is inserted between cotton and side wall from device edge, by vacuum side of blade court
On overlay on wet filter paper and cultivate；
Step 3: it is put into growth cabinet and trains again after the blade handled well and device are placed into lesser insect cage
It supports, growth cabinet condition setting are as follows: 26 ± 1 DEG C of temperature, humidity 70% ± 5%, photoperiod 14:10, illumination 18000lx；Often
Add within 5-7 days that a clear water is hatched to ovum or pupa is sprouted wings in tank body layer, when addition dustproof cover is opened after use plastic needle cylinder
Clear water is extracted to be injected into reserve liquid layer from filling opening；When device is set as multiple layer combination, adding on reserve liquid layer side wall is successively opened
Liquid mouth is added；Aleyrodid nymph, the adult hatched is directly tested or is transferred to and raised on new host plant.
9. using a kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device described in claim 1-7,
It is characterized in that, the method that the device feeds aleyrodid class parasitic wasp, comprising the following steps:
Step 1: distilled water flushing will be used after device wiping in the present invention using 75% alcohol, covers tightly reserve liquid layer (2) bottom sidewall
On filling opening (8) on dust seal cover (9) seal the filling opening of reserve liquid layer (2) bottom；Standard weighs bee on balance
Honey is added warm water and is configured to the hydromel that honey quality percentage is 10% ± 2%, is placed into room temperature after mixing evenly；
The height that configured hydromel is added in present apparatus reserve liquid layer 2/3 is extracted, is encased inside 1cm in the feeding layer with cotton column
Thick absorbent cotton；Then by feeding layer and reservoir bed, using turning, silk cooperation is close, and cotton column end portion is immersed in honey at this time
In water；Cotton is sprayed in cotton with the small watering can equipped with hydromel to moisten；
Step 2: the feeding device equipped with hydromel is placed into parasitic wasp insect cage every 10-15 days and is added in tank body layer
Add primary configured hydromel, by hydromel by honey quality score is 10% ± 2% configuration when addition, it is to be cooled to arrive room temperature
Hydromel is extracted with plastic needle cylinder afterwards, is injected into reserve liquid layer after dustproof cover is opened from filling opening, liquid still adds to liquid storage
2/3 position of layer volume；
Step 3: the degreasing cotton in the primary feeding layer of replacement in every 20-30 days during the feeding process, by old absorbent cotton when replacement
It removes, new absorbent cotton is encased inside in feeding layer, and is sprayed and moistened with the hydromel watering can for filling identical mass fraction, according to reality
Situation selects the 30-40 days cleaning present apparatus and carries out the replacement of cotton.
10. it is according to claim 9 a kind of suitable for aleyrodid class or the method for parasitic wasp class insects hatch, feeding device,
It is characterized in that, the mass fraction that the mould inhibitor configures are as follows: 1%-5% benzoic acid, 10%-15% sorbic acid, 0.1%-
0.5% calcium propionate, 0.05%-0.1% ethyl-para-hydroxybenzoate, it is remaining be clear water, dissolution mould inhibitor.
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| CN201811100453.6A CN109315356B (en) | 2018-09-20 | 2018-09-20 | Hatching and feeding device suitable for whitefly or parasitic wasp insects and application method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN115005165A (en) * | 2022-06-30 | 2022-09-06 | 云南农业大学 | A kind of separate feeding and observation device and using method of tomato leaf miner |
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| CN112655658A (en) * | 2020-12-30 | 2021-04-16 | 广东省科学院动物研究所 | Parasitic wasp feeding and collecting device and method |
| CN114521536A (en) * | 2022-02-18 | 2022-05-24 | 云南农业大学 | Integrated device for collecting, feeding and releasing natural enemies of orius minutus and using method |
| CN114521536B (en) * | 2022-02-18 | 2022-12-23 | 云南农业大学 | Integral device for collecting, raising and releasing natural enemy insects of the small flower stinkbug and its use method |
| CN115005165A (en) * | 2022-06-30 | 2022-09-06 | 云南农业大学 | A kind of separate feeding and observation device and using method of tomato leaf miner |
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