CN109306073A - Hydrophobic three-dimensional porous material and its preparation method and application and blood storage bag - Google Patents
Hydrophobic three-dimensional porous material and its preparation method and application and blood storage bag Download PDFInfo
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- 239000008280 blood Substances 0.000 title claims abstract description 134
- 210000004369 blood Anatomy 0.000 title claims abstract description 133
- 230000002209 hydrophobic effect Effects 0.000 title claims abstract description 92
- 239000011148 porous material Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 57
- 238000003860 storage Methods 0.000 title claims abstract description 46
- 235000000346 sugar Nutrition 0.000 claims abstract description 103
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 29
- 229920005573 silicon-containing polymer Polymers 0.000 claims abstract description 29
- 239000011343 solid material Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 239000008367 deionised water Substances 0.000 claims description 26
- 229910021641 deionized water Inorganic materials 0.000 claims description 26
- 229930006000 Sucrose Natural products 0.000 claims description 22
- 229920000742 Cotton Polymers 0.000 claims description 20
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 20
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 7
- 238000007711 solidification Methods 0.000 claims description 5
- 230000008023 solidification Effects 0.000 claims description 5
- 235000021552 granulated sugar Nutrition 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000003856 thermoforming Methods 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 2
- 125000000185 sucrose group Chemical group 0.000 claims description 2
- 210000003743 erythrocyte Anatomy 0.000 abstract description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 8
- 239000001301 oxygen Substances 0.000 abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 abstract description 6
- 230000001788 irregular Effects 0.000 abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 4
- 239000001569 carbon dioxide Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 description 36
- 239000004205 dimethyl polysiloxane Substances 0.000 description 27
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 27
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 26
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 26
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 26
- 238000000034 method Methods 0.000 description 19
- 239000007789 gas Substances 0.000 description 16
- 238000004090 dissolution Methods 0.000 description 11
- 239000003960 organic solvent Substances 0.000 description 9
- 238000002604 ultrasonography Methods 0.000 description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 8
- 239000011521 glass Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 238000004140 cleaning Methods 0.000 description 6
- 238000011049 filling Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000010412 perfusion Effects 0.000 description 5
- 230000017531 blood circulation Effects 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 239000004793 Polystyrene Substances 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- -1 methylsiloxane Chemical class 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 241000700157 Rattus norvegicus Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005266 casting Methods 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000007872 degassing Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- JZZIHCLFHIXETF-UHFFFAOYSA-N dimethylsilicon Chemical compound C[Si]C JZZIHCLFHIXETF-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
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- 238000012377 drug delivery Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
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- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004848 polyfunctional curative Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N titanium dioxide Inorganic materials O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/26—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
- A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
- A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
- A61J1/10—Bag-type containers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
- A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
- A61J1/14—Details; Accessories therefor
- A61J1/1443—Containers with means for dispensing liquid medicaments in a filtered or sterile way, e.g. with bacterial filters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
- A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
- A61J1/14—Details; Accessories therefor
- A61J1/1468—Containers characterised by specific material properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/50—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition
- B01D29/56—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2201/00—Foams characterised by the foaming process
- C08J2201/04—Foams characterised by the foaming process characterised by the elimination of a liquid or solid component, e.g. precipitation, leaching out, evaporation
- C08J2201/042—Elimination of an organic solid phase
- C08J2201/0422—Elimination of an organic solid phase containing oxygen atoms, e.g. saccharose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2383/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen, or carbon only; Derivatives of such polymers
- C08J2383/04—Polysiloxanes
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- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
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- Materials For Medical Uses (AREA)
Abstract
The present invention relates to area of medical devices, disclose a kind of hydrophobic three-dimensional porous material and its preparation method and application and a kind of blood storage bag, the preparation method of the hydrophobic three-dimensional porous material includes: that (1) using water-soluble sugar prepares porous structure sugar template；(2) dimethyl silicone polymer is poured into porous structure sugar template and is solidified；(3) the porous structure sugar template in step (2) obtained solid material is removed.The preparation method of hydrophobic three-dimensional porous material provided by the invention is easy to operate, hydrophobic three-dimensional porous material organic solvent-free residual at low cost and obtained, hydrophobic three-dimensional porous material made from the preparation method of hydrophobic three-dimensional porous material provided by the invention has irregular pore structure for improving blood sample partial pressure of oxygen more significantly in blood storage bag, carbon dioxide partial pressure is reduced, and improves erythrocyte deformability.
Description
Technical field
The present invention relates to area of medical devices, and in particular to a kind of hydrophobic three-dimensional porous material and its preparation method and application
And a kind of blood storage bag.
Background technique
Hydrophobic three-dimensional porous structure is widely present in nature and organism, has the characteristics that high-specific surface area.It is hydrophobic
Three-dimensional porous structure is internal connection and outside and the important channel for carrying out substance and energy exchange.It is situated between in bioreactor, electricity
Matter, sensor, drug delivery, substance separation, catalysis etc. have huge application prospect.
The preparation of classical hydrophobic three-dimensional porous material, generally requiring will be by glass, TiO2Or the materials such as macromolecule are made
Microballoon accumulation, and connection under the conditions of high temperature (such as glass: 500-1600 DEG C) forms template, is then filled with material, Gu
Change, finally dissolves bead using organic solvent (such as: hydrofluoric acid), ultimately form hydrophobic three-dimensional porous material.
Such method uses organic solvent more, and when being used for biomedical products, remaining organic solvent can generate potential
Harm.In addition, existing preparation method is expensive in the prevalence of Preparation equipment, preparation condition is harsh, technology of preparing requires high, process
The problems such as complicated cumbersome.Therefore it is urgent to provide propose a kind of improved hydrophobic three-dimensional porous structure preparation method.
Summary of the invention
It is remaining the purpose of the invention is to overcome in hydrophobic three-dimensional porous structure preparation method of the existing technology
Organic solvent can generate potential hazard, and Preparation equipment is expensive, preparation condition is harsh, technology of preparing requires height, process complexity numerous
Trivial problem provides a kind of preparation method of hydrophobic three-dimensional porous material, the hydrophobic three-dimensional porous material as made from the preparation method
Material, the application of the hydrophobic three-dimensional porous material and a kind of blood storage bag.The preparation of hydrophobic three-dimensional porous material provided by the invention
Method is easy to operate, hydrophobic three-dimensional porous material organic solvent-free residual at low cost and obtained.
The present inventor has found in the course of the research, in area of medical devices, using irregular hydrophobic three-dimensional porous
Material is more advantageous to performance properties of product, in addition, in area of medical devices, minimal residue in material high to product requirement
Fatal harm will be brought to patient body.The present inventor is in the course of the research it has furthermore been found that using water-soluble sugar
Porous structure sugar template is prepared, it is not only easy to operate, and high temperature is not needed when removal sugar template, organic solvent is not needed yet, is made
Organic solvent-free residual in the hydrophobic three-dimensional porous material obtained.
Based on this, the present invention provides a kind of preparation method of hydrophobic three-dimensional porous material, this method comprises:
(1) porous structure sugar template is prepared using water-soluble sugar；
(2) dimethyl silicone polymer is poured into porous structure sugar template and is solidified；
(3) the porous structure sugar template in step (2) obtained solid material is removed.
The present invention also provides the hydrophobic three-dimensional porous materials as made from above-mentioned preparation method.Hydrophobic three-dimensional provided by the invention
Porous material organic solvent-free residual.
The present invention also provides application of the above-mentioned hydrophobic three-dimensional porous material in blood storage bag.
The present invention also provides a kind of blood storage bag, which includes bag body and filter core, and the filter core includes the first filter core, the
Two filter cores and third filter core；
Bag body internal cavity is divided into room under blood bag upper chamber and blood bag by first filter core, and is arranged on first filter core
There is microchannel that room under blood bag upper chamber and blood bag is interconnected；
Second filter core is located at chamber interior in blood bag, and the third filter core is located at chamber interior under blood bag；
The filter core is made of above-mentioned hydrophobic three-dimensional porous material.
The preparation method of hydrophobic three-dimensional porous material provided by the invention has the advantage that compared with prior art
(1) preparation method of hydrophobic three-dimensional porous material provided by the invention is easy to operate, at low cost and obtained hydrophobic
Three-dimensional porous material organic solvent-free residual；
(2) hydrophobic three-dimensional porous material made from the preparation method of hydrophobic three-dimensional porous material provided by the invention has not
Regular pore structure reduces carbon dioxide partial pressure, and improve for improving blood sample partial pressure of oxygen in blood storage bag more significantly
Erythrocyte deformability.
Detailed description of the invention
Fig. 1 is the scanning electron microscope (SEM) photograph for the hydrophobic three-dimensional porous material that preparation example 1 is prepared；
Fig. 2 is a kind of blood storage bag for specific embodiment that embodiment 1 provides.
Description of symbols
1- bag body 21- the first filter core the second filter core of 22-
Room under 23- third filter core 3- blood bag upper chamber 4- blood bag
5- microchannel 6- blood transfusion port 7- upper hanging hole
8- lower hanging hole
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
A kind of preparation method of hydrophobic three-dimensional porous material, this method comprises:
(1) porous structure sugar template is prepared using water-soluble sugar；
(2) dimethyl silicone polymer is poured into porous structure sugar template and is solidified；
(3) the porous structure sugar template in step (2) obtained solid material is removed.
In accordance with the present invention it is preferred that solubility of the water-soluble sugar at 25 DEG C in water is 1.9-2.1g sugar/g water,
Further preferably 1.95-2.05g sugar/g water.
, according to the invention it is preferred to which the water-soluble sugar is sucrose.In order to further control hydrophobic three-dimensional porous material
Pore structure, the preferably described water-soluble sugar contain sugar nibs and cotton white sugar.
In the present invention, it is preferred to which the partial size of the sugar nibs is 300-1250 μm, the partial size of the cotton white sugar is 100-500
μm.Partial size is in terms of the maximum cross section diameter of particle.
In the present invention, sugar nibs and cotton can be selected by the requirement of the pore-size distribution to hydrophobic three-dimensional porous material
The usage ratio of white sugar.Preferably, on the basis of the total amount of the water-soluble sugar, the content of the sugar nibs is 20-80 weight
% is measured, the content of cotton white sugar is 20-80 weight %；It is further preferred that the content of the sugar nibs is 20-40 weight %, cotton
The content of white sugar is 60-80 weight %.
The product of different pore size distribution in order to obtain, the present invention can also include grinding to sugar nibs and cotton white sugar,
Porous structure sugar template then is prepared according to the method for step (1) after obtaining specific partial size.Those skilled in the art are according to this
Disclosure of invention can be selected accordingly according to actual needs, and details are not described herein by the present invention.
The present invention can realize hydrophobic three-dimensional porous material not by using the cotton white sugar and sugar nibs of different-grain diameter
The preparation of regular pore structure.
And in the prior art, it mostly uses the glass pellet of rule to carry out accumulation and forms template, carried out not using glass pellet
The preparation process of the hydrophobic three-dimensional porous material of regular pore structure is complicated, needs to prepare the glass pellet of different size, also,
During subsequent stripper plate, the removing of glass pellet needs high temperature and organic solvent, complex process and has dissolvent residual.
As long as porous structure sugar template will be prepared using water-soluble sugar in the present invention, to specific embodiment party
There is no particular limitation for formula, it is preferable that step (1) include: after water-soluble sugar is mixed with water thermoforming obtain porous structure
Sugared template；It is further preferred that the dosage of water is 0.5-5mL, preferably 1-3mL relative to 100g water-soluble sugar；Further
Preferably, the condition of the thermoforming include: temperature be 70-120 DEG C, preferably 80-120 DEG C, time 0.5-4h, preferably
For 1-2h.
The specific embodiment of step (1) of the present invention may include: according to different by sugar nibs and cotton white sugar to hydrophobic
The requirement of the pore-size distribution of three-dimensional porous material mixes in beaker in varing proportions；By 100g water-soluble sugar/0.5-5mL ratio
Water is added in example, and is uniformly mixed with water-soluble sugar；The substance being mixed to get is poured into the mold of required shape, and by mold pressure
Tightly；Mold is put into 70-100 DEG C of heating 0.5-4h in baking oven；After being cooled to room temperature, the mold of porous structure sugar template will be filled
It is placed in vacuum evacuation device.
In the present invention, as long as dimethyl silicone polymer is poured into porous structure sugar template, to its specific embodiment party
There is no particular limitation for formula, it is preferable that step (2) (namely mode of perfusion) includes: by dimethyl silicone polymer and curing agent
Then mixing vacuumizes to obtain mixture by first, the mixture is contacted with porous structure sugar template (thus real
Now it is perfused), then in 30-45 DEG C of solidification 2-5h.There is no particular limitation for the specific embodiment vacuumized to described first, only
It wants that bubble can be removed, preferably the first time vacuumized is 20-60min.
There is no particular limitation for dosage of the present invention to curing agent, can be hardener dose commonly used in the art,
For example, the mass ratio of dimethyl silicone polymer and curing agent can be 8-12:1.The curing agent can be for arbitrarily with poly- two
The matching used curing agent of methylsiloxane, there is no particular limitation to this by the present invention, such as can be to be commercially available from DOW CORNING public affairs
Take charge of the complete curing agent that the trade mark is 184 products.
A preferred embodiment of the invention, the contact carry out under the second vacuumized conditions, the contact
Time be 7-17h, the temperature of contact is 10-20 DEG C.
First vacuumizes for extracting the bubble in dimethyl silicone polymer and curing agent out in the present invention；Second vacuumizes use
In removing the bubble in porous structure sugar template, and the hole of porous structure sugar template is entered convenient for dimethyl silicone polymer
In.
In accordance with the present invention it is preferred that the mass ratio of dimethyl silicone polymer and water-soluble sugar is 1-3:1, further preferably
It is still more preferably 1.5-2:1 for 1.5-2.5:1.It is more conducive to using the preferred embodiment of the present invention hydrophobic three
Irregular pore structure is formed in dimension porous material.
The specific embodiment of step (2) of the present invention may include: that dimethyl silicone polymer and curing agent are with mass ratio
8-12:1 mixing, vacuumizes 20-60min to bubble-free and obtains mixture；Mixture addition is filled into porous structure sugar mould
It in the mold of plate, contacts under vacuum-pumping conditions, is then placed polymerization and complete (can be 8-12h), finally at 30-45 DEG C
Heat 2-5h.
During dimethyl silicone polymer and porous structure sugar template contacts, the process vacuumized is not limited particularly
It is fixed, can be and all and after porous structure sugar template contacts, once vacuumized dimethyl silicone polymer, be also possible to by
It is vacuumized after part dimethyl silicone polymer and porous structure sugar template contacts, remainder poly dimethyl silicon is then added
Oxygen alkane continues to vacuumize, and there is no particular limitation to this by the present invention, as long as can be by the air in porous structure sugar template hole
Extraction, will be in the hole of dimethyl silicone polymer filling porous structure sugar template.
In step (3) of the present invention, as long as the porous structure sugar template in step (2) obtained solid material is removed,
Such as it can be under the conditions of 35-50 DEG C, using deionized water by the porous structure sugar template in step (2) obtained solid material
Dissolution removes.
Step (3) of the present invention can carry out on the conventional equipment of this field, for example, can be enterprising in supersonic wave cleaning machine
Row is more advantageous to the dissolution for accelerating sugar using ultrasonic washing.
A preferred embodiment of the invention in ultrasonic procedure, changes a deionized water every 30-120min,
Ultrasound is continued using fresh deionized water, until the porous structure sugar template in step (2) obtained solid material is completely removed.
A kind of specific embodiment according to the present invention, this method can also include: dried after step (3)
Journey.
As long as the drying process dries step (3) products therefrom, it is preferable that the condition of the drying includes:
Temperature is 60-100 DEG C, time 0.5-3h.
The present invention also provides hydrophobic three-dimensional porous materials made from above-mentioned preparation method.The material is tied with irregular hole
Structure.
Organic solvent-free remains in hydrophobic three-dimensional porous material provided by the invention, especially suitable for biomedicine field.
Therefore, the present invention also provides hydrophobic three-dimensional porous material made from above-mentioned preparation method or hydrophobic three-dimensional provided by the invention are more
Application of the Porous materials in blood storage bag.
The present invention also provides a kind of blood storage bag, which includes bag body 1 and filter core, and the filter core has hydrophobic three-dimensional
Porous structure, the filter core include the first filter core 21, the second filter core 22 and third filter core 23；First filter core 21 is by bag body 1
Internal cavity is divided into room 4 under blood bag upper chamber 3 and blood bag, and is provided with microchannel 5 on first filter core 21 and makes in blood bag
Room 4 is interconnected under room 3 and blood bag；Second filter core 22 is located inside blood bag upper chamber 3, and the third filter core 23 is located at blood bag
Inside lower room 4, the filter core is made of above-mentioned hydrophobic three-dimensional porous material.
In the present invention, in the absence of explanation to the contrary, the noun of locality used such as " top, bottom, between, it is intermediate "
Typically refer to reference to top shown in the drawings, bottom, between, it is intermediate, the noun of locality used such as " inside and outside " refers to relative to each portion
The profile of part itself it is inside and outside.In addition, using the vertical direction of blood storage bag use state as vertical direction.
Hydrophobic three-dimensional porous material provided by the invention is used in blood storage bag, the gas that can improve storage of red blood cells is handed over
State is changed, improves sample partial pressure of oxygen more significantly, reduces carbon dioxide partial pressure, and improve erythrocyte deformability.
A kind of most preferred embodiment according to the present invention, the blood storage bag include bag body 1 and filter core, and the filter core has
Hydrophobic three-dimensional porous structure, the filter core include the first filter core 21, the second filter core 22 and third filter core 23；First filter core 21
1 internal cavity of bag body is divided into room 4 under blood bag upper chamber 3 and blood bag, and is provided with microchannel 5 on first filter core 21 and makes
Room 4 is interconnected under blood bag upper chamber 3 and blood bag；Second filter core 22 is located inside blood bag upper chamber 3, the third filter core 23
Under blood bag inside room 4, the filter core is made of above-mentioned hydrophobic three-dimensional porous material, the preparation of the hydrophobic three-dimensional porous material
Method includes: that (1) uses water-soluble sugar to prepare porous structure sugar template；(2) dimethyl silicone polymer is poured into porous structure
In sugared template and solidify；(3) the porous structure sugar template in step (2) obtained solid material is removed.
The present invention is filtered by the way that the filter core with hydrophobic three-dimensional porous structure is arranged in blood storage bag internal cavity, and first
Microchannel is arranged on core 21 can guarantee that the flowing of blood and red blood cell carry out gas exchanges.With hydrophobic three-dimensional porous structure
Filter core in store gas, when the blood in bag body from blood bag upper chamber rely on gravity, be enclosed with hydrophobic three by surrounding
The microchannel of dimension porous structure flow to room under blood bag from blood bag upper chamber, and red blood cell carries out effectively in hydrophobic three-dimensional porous structure
Gas exchanges, blood flow to room under blood bag from blood bag upper chamber and air pressure both made to change, and gas is oppressed in lower room, edge
Hydrophobic three-dimensional porous structure gas-bearing formation uplink is inverted when blood all flows into lower room, then by blood storage bag, is arranged in blood bag upper chamber 3
The second internal filter core 22 and the third filter core 23 being arranged under blood bag inside room 4 can be absorbed and release gas, facilitate blood
Effective progress of liquid circulation.
Actually preparation when, can by bag body 1 by the adhesive of bio-safety and be arranged by above-mentioned hydrophobic three
The filter core that porous material is constituted is tieed up, then bag body is sealed by the method for hot pressing.
The present invention is not particularly limited the number of the microchannel 5, can be in blood bag as long as can satisfy blood
It circulates between room 4 under upper chamber 3 and blood bag, at least two microchannels 5 is provided on preferably described first filter core 21, into one
3 microchannels 5 are provided on step preferably the first filter core 21.It significantly more efficient can be controlled using this kind of preferred embodiment
The speed of blood flow is more advantageous to red blood cell and carries out gas exchanges.
A preferred embodiment of the invention, the cross-sectional diameter of the microchannel 5 are 0.2-1mm.
The present invention is not particularly limited the method that microchannel 5 is arranged on the first filter core 21, those skilled in the art
Member can suitably select as the case may be, for example, can pass through the material in the hydrophobic three-dimensional porous structure for preparing the first filter core
When material, microchannel 5 is reserved on material, material (or commercially available material) can also be first prepared, then be punched to obtain
The microchannel 5.
In order to the significantly more efficient volume using the blood storage bag, preferably described first filter core 21 will be empty in bag body 1
Chamber is divided into room 4 under volume equal blood bag upper chamber 3 and blood bag.
A preferred embodiment of the invention, in the vertical direction, the thickness of first filter core 21 with it is described
The ratio of the height of bag body 1 is 1:7.5-14.Using this kind of preferred embodiment, it is more advantageous to the storage blood for maximally utilizing blood storage bag
Space, and be more conducive to guaranteeing the progress of red blood cell gas exchanges.
A preferred embodiment of the invention, second filter core 22 and third filter core 23 are vertically arranged, and
First filter core 21, the second filter core 22 and third filter core 23 are linked together namely gas can be in the first filter core 21, the second filter core 22
It is flowed freely in third filter core 23.
A preferred embodiment of the invention, second filter core 22 and third filter core 23 are along the bag body 1
Edge sealing setting.This kind of preferred embodiment more saves the volume of internal cavity, improves the storage blood volume of blood storage bag.
A kind of specific embodiment according to the present invention, second filter core 22 can extend to the end of bag body 1 along bag body 1
Portion；The third filter core 23 can extend to the other end of bag body 1 along the edge sealing of bag body 1；Second filter core 22 and third
Filter core 23 can be arranged along same vertical axis, can also be oppositely arranged (that is, the second filter core 22 and third filter core 23 are respectively with
The both ends of one filter core 21 are in contact), there is no particular limitation to this by the present invention, preferably described second filter core 22 and third filter core 23
It is oppositely arranged.
In accordance with the present invention it is preferred that being provided with blood transfusion port 6 at the top of the bag body 1.
In accordance with the present invention it is preferred that being provided with upper hanging hole 7 at the top of the bag body 1,1 bottom of bag body is provided with lower extension
Hole 8.It is more convenient for the use of blood storage bag using this kind of preferred embodiment.
A kind of specific embodiment of blood storage bag provided by the invention is illustrated below in conjunction with Fig. 2.
As shown in Fig. 2, it (is highly 14cm) and filter core that the blood storage bag, which includes bag body 1,；First filter core 22 (with a thickness of 2cm) will
1 internal cavity of bag body is divided into room 4 under volume equal blood bag upper chamber 3 and blood bag, is provided with 3 diameters on the first filter core 22 and is
The microchannel 5 of 0.5mm, the material of the filter core are that dimethyl silicone polymer provided by the invention (PDMS) is hydrophobic three-dimensional porous
Material；Second filter core 22 be vertically arranged inside blood bag upper chamber 3 along the right edge sealing of bag body 1, and extend upwardly to 1 top of bag body,
It third filter core 23 and is vertically arranged under blood bag inside room 4 along the left edge sealing of bag body 1, and extends downward into 1 bottom of bag body, then led to
Cross hot press sealed bag body 1.The middle position at 1 top of bag body is provided with upper hanging hole 7, the interposition of 1 bottom of bag body
It installs and is equipped with lower hanging hole 8.
Blood transfusion port 6 is provided at the top of bag body 1, blood flows into blood bag upper chamber 3 from blood transfusion port 6, passes through by gravity micro-
Circulation road 5 flow to room 4 under blood bag from blood bag upper chamber 3, and red blood cell carries out effective gas in hydrophobic three-dimensional porous structure in blood
Body exchange, blood flow to room 4 under blood bag from blood bag upper chamber 3 and the two air pressure are changed, and gas is oppressed in lower room, along thin
Water three-dimensional porous structure gas-bearing formation uplink is inverted when blood all flows into room 4 under blood bag, then by blood storage bag, realizes following for blood
Ring, the setting of the second filter core 22 and third filter core 23 enable in blood storage bag blood effectively under blood bag upper chamber 3 and blood bag
It is flowed between room 4, the locking and flowing of gas has been better achieved in this kind of mode.
Below by way of the specific embodiment implementation process that the present invention will be described in detail and generated beneficial effect, it is intended to help
Where reader more clearly understands Spirit Essence of the invention, but any restriction cannot be constituted to practical range of the invention.
In following embodiment, cotton white sugar is commercially available from romote antiquity sugar industry Co., Ltd, trade name romote antiquity top grade white granulated sugar, partial size
It is 100-500 μm.Sugar nibs are commercially available from romote antiquity sugar industry Co., Ltd, trade name romote antiquity top grade white granulated sugar, partial size 300-
1250μm；Dimethyl silicone polymer is commercially available from Dow Corning Corporation, and 184 casting glues of trade name SYLGARD/silicon rubber/PDMS is poly-
Dimethyl siloxane；Curing agent is commercially available from Dow Corning Corporation, is 184 casting glues of SYLGARD/silicon rubber/PDMS poly dimethyl silicon
The auxiliary products of oxygen alkane.
Preparation example 1
(1) preparation of porous structure sugar template
30g sugar nibs are taken to mix in beaker with 70g cotton white sugar, by 100g water-soluble sugar (sugar nibs and the total matter of cotton white sugar
Amount) the ratio addition deionized water of 1mL deionized water is added and is uniformly mixed；The substance being mixed to get is poured into mold (self-control Z
Subtype PMMA mold, filter core shape in specific blood storage bag as shown in Figure 2, similarly hereinafter) in, and mold is compressed；Mold is put into baking
In case, in 80 DEG C of heating 1h；After being cooled to room temperature, the mold for filling porous structure sugar template is placed in vacuum evacuation device；
(2) perfusion of dimethyl silicone polymer
With curing agent in mass ratio it is that 10:1 is mixed by dimethyl silicone polymer (150g), then vacuumizes 30min removal
Bubble obtains mixture, the mixture is all poured into the mold for filling porous structure sugar template and then vacuumizes 2.5h,
Then after 20 DEG C of placement 12h, in 40 DEG C of solidification 3h, solid material is obtained；
(3) removing of sugared template
The solid material is put into beaker, be added 500mL deionized water, in supersonic wave cleaning machine at 40 DEG C into
Row ultrasound sugar dissolution, pours out deionized water in beaker every 1h, and fresh deionized water is added and continues ultrasonic dissolution, ultrasound sugar is molten
After solving 4h, deionized water is poured out, 2h is then heated at 80 DEG C in baking oven and obtains hydrophobic three-dimensional porous PDMS material S-1.
The scanning electron microscope (SEM) photograph of hydrophobic three-dimensional porous PDMS material S-1 is as shown in Figure 1, it can be seen from the figure that hydrophobic three
It ties up and forms irregular pore structure inside porous PDMS material S-1.
Preparation example 2
(1) preparation of porous structure sugar template
20g sugar nibs are taken to mix in beaker with 80g cotton white sugar, by 100g water-soluble sugar (sugar nibs and the total matter of cotton white sugar
Amount) the ratio addition deionized water of 2mL deionized water is added and is uniformly mixed；The substance being mixed to get is poured into mold, and
Mold is compressed；Mold is put into baking oven, in 100 DEG C of heating 2h；After being cooled to room temperature, porous structure sugar template will be filled
Mold is placed in vacuum evacuation device；
(2) perfusion of dimethyl silicone polymer
With curing agent in mass ratio it is that 8:1 is mixed by dimethyl silicone polymer (180g), then vacuumizes 30min and go degasification
Bubble, obtains mixture, mixture described in half is poured into the mold for filling porous structure sugar template and then vacuumizes 1h, will remained
Remaining part point continues to pour into mold and then vacuumizes 1h, then after 20 DEG C of placement 8h, in 30 DEG C of solidification 5h, obtains solid material；
(3) removing of sugared template
The solid material is put into beaker, be added 500mL deionized water, in supersonic wave cleaning machine at 40 DEG C into
Row ultrasound sugar dissolution, pours out deionized water in beaker every 1h, and fresh deionized water is added and continues ultrasonic dissolution, ultrasound sugar is molten
After solving 4h, deionized water is poured out, 2h is then heated at 80 DEG C in baking oven and obtains hydrophobic three-dimensional porous PDMS material S-2.
The scanning electricity of the scanning electron microscope (SEM) photograph of hydrophobic three-dimensional porous PDMS material S-2 and hydrophobic three-dimensional porous PDMS material S-1
Mirror figure is similar.
Preparation example 3
(1) preparation of porous structure sugar template
40g sugar nibs are taken to mix in beaker with 60g cotton white sugar, by 100g water-soluble sugar (sugar nibs and the total matter of cotton white sugar
Amount) the ratio addition deionized water of 3mL deionized water is added and is uniformly mixed；The substance being mixed to get is poured into mold, and
Mold is compressed；Mold is put into baking oven, in 120 DEG C of heating 1h；After being cooled to room temperature, porous structure sugar template will be filled
Mold is placed in vacuum evacuation device；
(2) perfusion of dimethyl silicone polymer
With curing agent in mass ratio it is that 12:1 is mixed by dimethyl silicone polymer (200g), then vacuumizes 45min removal
Bubble obtains mixture, mixture described in half is poured into the mold for filling porous structure sugar template and then vacuumizes 1h, will
Remainder continues to pour into mold and then vacuumizes 1h, then after 20 DEG C of placement 10h, in 45 DEG C of solidification 2h, obtains solid material
Material；
(3) removing of sugared template
The solid material is put into beaker, be added 500mL deionized water, in supersonic wave cleaning machine at 35 DEG C into
Row ultrasound sugar dissolution, pours out deionized water in beaker every 1h, and fresh deionized water is added and continues ultrasonic dissolution, ultrasound sugar is molten
After solving 4h, deionized water is poured out, 2h is then heated at 80 DEG C in baking oven and obtains hydrophobic three-dimensional porous PDMS material S-3.
The scanning electricity of the scanning electron microscope (SEM) photograph of hydrophobic three-dimensional porous PDMS material S-3 and hydrophobic three-dimensional porous PDMS material S-1
Mirror figure is similar.
Preparation example 4
According to the method for preparation example 1, the difference is that, sugar nibs are replaced with to the cotton white sugar of phase homogenous quantities, obtain hydrophobic three
Tie up porous PDMS material S-4.The scanning electron microscope (SEM) photograph of hydrophobic three-dimensional porous PDMS material S-4 shows that the relatively hydrophobic three-dimensional of its pore structure is more
Hole PDMS material S-1 is more regular.
Preparation example 5
According to the method for preparation example 1, the difference is that, cotton white sugar is replaced with to the sugar nibs of phase homogenous quantities, obtains hydrophobic three
Tie up porous PDMS material S-5.The scanning electron microscope (SEM) photograph of hydrophobic three-dimensional porous PDMS material S-5 shows that the relatively hydrophobic three-dimensional of its pore structure is more
Hole PDMS material S-1 is more regular.
Prepare comparative example 1
(1) preparation of porous structure template
Diameter 1mm polystyrene sphere 100g is tightly packed into self-control glass mold.
(2) perfusion of dimethyl silicone polymer
With curing agent in mass ratio it is that 10:1 is mixed by 150g dimethyl silicone polymer, then vacuumizes 45min and go degasification
Bubble, obtains mixture, mixture described in half is poured into the mold for filling porous structure polystyrene sphere template and is then taken out
Vacuum 1h continues remainder to pour into mold and then vacuumizes 1h, solid in 45 DEG C then after (25 DEG C) placement 10h of room temperature
Change 2h, obtains solid material；
(3) removing of polystyrene sphere template
The solid material is put into beaker, 300mL acetone is added, is surpassed at 35 DEG C in supersonic wave cleaning machine
Sound dissolution, pours out acetone in beaker every 1h, addition fresh acetone continuation ultrasonic dissolution, after ultrasound sugar dissolution 4h, pours out
Acetone.Then the solid material is put into beaker, 500mL deionized water is added, in supersonic wave cleaning machine at 35 DEG C
It is cleaned by ultrasonic, is poured out deionized water in beaker every 1h, fresh deionized water is added and continues to be cleaned by ultrasonic, ultrasound sugar is clear
After washing 4h, deionized water is poured out, then heats 2h at 80 DEG C in baking oven, obtains hydrophobic three-dimensional porous PDMS material D-1.It is hydrophobic
The preparation of three-dimensional porous PDMS material D-1 is needed using organic solvent, in hydrophobic three-dimensional porous PDMS material D-1 inevitably
Have a residual of a little organic solvent, and preparation process is complicated, the pore structure of obtained hydrophobic three-dimensional porous PDMS material D-1 compared with
For rule.
Embodiment 1
The present embodiment is used for the effect for illustrating to apply the hydrophobic three-dimensional porous PDMS material of above-mentioned preparation in blood storage bag.
As shown in Fig. 2, it (is highly 14cm, width 11cm) and filter core that blood storage bag, which includes bag body 1,；First filter core 22 is (thick
Degree is 2cm) 1 internal cavity of bag body is divided into room 4 under volume equal blood bag upper chamber 3 and blood bag, 3 are provided on the first filter core 22
A diameter is the microchannel 5 of 0.5mm, and the material of the filter core is respectively hydrophobic three-dimensional porous PDMS made from above-mentioned preparation example
Material S-1 to S-5 and D-1；Second filter core 22 (width (horizontal direction) is 1cm) is vertically arranged at along the right edge sealing of bag body 1
Inside blood bag upper chamber 3, and 1 top of bag body is extended upwardly to, (width (horizontal direction) is for 1cm) and along bag body 1 for third filter core 23
Left edge sealing is vertically arranged under blood bag inside room 4, and extends downward into 1 bottom of bag body, then closes bag body 1 by hot press.
The middle position at 1 top of bag body is provided with upper hanging hole 7, and the middle position of 1 bottom of bag body is provided with lower hanging hole 8.
Test example 1
In order to illustrate the technical effect that hydrophobic three-dimensional porous material provided by the invention is applied in blood storage bag, to the present invention
The blood storage performance of the blood storage bag of offer is tested.Test method is as follows:
(1) Wistar rat extracting blood (be related to animal and take blood, Ethics Committee ratifies): rat body test tube of hepari, neck
Arterial cannulation, in the centrifuge tube of autonomous bloodletting to the CPDA-1 that 1.4mL is housed, every pipe 10mL takes 8 Wistar rat serums altogether,
Total amount for taking blood 100ml；
(2) leukocyte depletion filter filtrating leukocytes are used, gained blood is centrifuged 15min under centrifuge 400g revolving speed, are inhaled
It walks supernatant and prepares Red Blood Cells Concentrate；
(3) T0 (fresh blood) blood sample is taken, vim and vigour, blood routine are detected, leaves and takes 150 μ L of sample, 400 μ L Hypertonic NaCl solutions are added
(1.5%) it mixes afterwards, after measuring ctHb and Hct using cellanalyzer, 3000rpm is centrifuged 10min, takes sorting on 150 μ L
It does not freeze, free hemoglobin to be measured；
(4) it respectively takes step (2) that Red Blood Cells Concentrate 5mL is made, is respectively placed in 8 blood storage bags provided by the invention and 8 general
In logical blood storage bag (Nangeer Biological Medicine Co., Ltd., Sichuan, standard: GB4232.1), it is placed in 4 DEG C of refrigerators and saves,
In 4 blood storage bags provided by the invention, 4 common blood bags be placed on shaking table；
(5) keep sample to storage 1h, 12h, sample detection blood routine for 24 hours, vim and vigour respectively and survey free hemoglobin concentration.
China TCM Academy of Sciences Xiyuan Hospital angiocarpy laboratory testing is entrusted in the test of deformable index, blood routine, vim and vigour.
Blood routine: blood routine various parameter values are measured with automatic hematology analyzer CD-1200.
Vim and vigour: blood gas index is measured with portable blood gas analyzer radiometer abl90Flex.
Erythrocyte deformability, unit: %.For the instrument used for LBY-BX2 type erythrocytic function laser determination instrument, Beijing is general
The production of Li Sheng company.Detection of RBC Deformability is measured using laser diffractometry, tests structure, red blood cell suspension using cone-plate
Liquid uses concentration for the PBS solution (pH 7.4) of 15% polyvinylpyrrolidone (K-30, PVP), by diluted red blood cell suspension
Liquid injects on rotary table, and when test, shearing rate is continuously increased by 100s -1-1100s -1, keeps shearing force suffered by red blood cell also continuous
Increase, the form of cell is gradually elongated to ellipse by circle, and computer calculates the index of RBC deformation automatically.It is hydrophobic
The blood routine of blood storage bag made from three-dimensional porous PDMS material S-1, the measurement result of vim and vigour are as shown in table 1, erythrocyte deformability
Measurement result and table 2 shown in.
Blood after 24 hours of blood storage bag made from hydrophobic three-dimensional porous PDMS material S-2 to S-5 and D-1 standing
Blood routine, the measurement result of vim and vigour are as shown in table 3.
Table 1
Note: in table 1, S represents blood bag provided by the invention, and D indicates above-mentioned common blood bag；1,12,24 storage is respectively represented
Time
Table 2
Table 3
The preparation method of hydrophobic three-dimensional porous material provided by the invention, the sugared mould of not only easy to operate, at low cost and removal
High temperature is not needed when plate, does not need organic solvent yet, and organic solvent-free remains in hydrophobic three-dimensional porous material obtained；The present invention
The hydrophobic three-dimensional porous material provided is applied to improve blood sample partial pressure of oxygen in blood storage bag more significantly, reduces carbon dioxide
Partial pressure, and improve erythrocyte deformability.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In skill of the invention
In art conception range, can with various simple variants of the technical solution of the present invention are made, including each technical characteristic with it is any its
Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, is belonged to
Protection scope of the present invention.
Claims (10)
1. a kind of preparation method of hydrophobic three-dimensional porous material, which is characterized in that the preparation method includes:
(1) porous structure sugar template is prepared using water-soluble sugar；
(2) dimethyl silicone polymer is poured into porous structure sugar template and is solidified；
(3) the porous structure sugar template in step (2) obtained solid material is removed.
2. preparation method according to claim 1, wherein solubility of the water-soluble sugar at 25 DEG C in water is
1.9-2.1g sugar/g water；
Preferably, the water-soluble sugar is sucrose, and the further preferred water-soluble sugar contains sugar nibs and cotton white sugar, described thick
The partial size of granulated sugar is 300-1250 μm, and the partial size of the cotton white sugar is 100-500 μm；
Preferably, on the basis of the total amount of the water-soluble sugar, the content of the sugar nibs is 20-80 weight %, cotton white sugar
Content is 20-80 weight %.
3. preparation method according to claim 1, wherein step (1) includes: to be heated into after mixing water-soluble sugar with water
Shape obtains porous structure sugar template；
Preferably, relative to 100g water-soluble sugar, the dosage of water is 0.5-5mL；
Preferably, it is 70-120 DEG C that the condition of the thermoforming, which includes: temperature, time 0.5-4h.
4. preparation method according to claim 1, wherein step (2) includes: to mix dimethyl silicone polymer and curing agent
It closes, then vacuumizes to obtain mixture by first, the mixture is contacted with porous structure sugar template, is then existed
30-45 DEG C of solidification 2-5h；
Preferably, the mass ratio of dimethyl silicone polymer and curing agent is 8-12:1；
Preferably, the contact carries out under the second vacuumized conditions, and the time of the contact is 7-17h, and the temperature of contact is
10-20℃。
5. preparation method described in any one of -4 according to claim 1, wherein dimethyl silicone polymer and water-soluble sugar
Mass ratio is 1-3:1, preferably 1.5-2.5:1.
6. preparation method described in any one of -5 according to claim 1, wherein step (3) includes: in 35-50 DEG C of condition
Under, the porous structure sugar template in step (2) obtained solid material is dissolved using deionized water and is removed.
7. hydrophobic three-dimensional porous material made from preparation method described in any one of claim 1-6.
8. hydrophobic three-dimensional porous material made from preparation method described in any one of claim 1-6 or claim 7 institute
Application of the hydrophobic three-dimensional porous material stated in blood storage bag.
9. a kind of blood storage bag, which is characterized in that the blood storage bag includes bag body (1) and filter core, and the filter core includes the first filter core
(21), the second filter core (22) and third filter core (23)；
Bag body (1) internal cavity is divided into room (4) under blood bag upper chamber (3) and blood bag by first filter core (21), and described first
Microchannel (5) is provided on filter core (21) makes room (4) under blood bag upper chamber (3) and blood bag be interconnected；
Second filter core (22) is located at blood bag upper chamber (3) inside, and the third filter core (23) is located at internal under blood bag room (4)；
Hydrophobic three-dimensional porous material or right made from filter core preparation method as described in any one of claim 1-6
It is required that hydrophobic three-dimensional porous material described in 7 is constituted.
10. blood storage bag according to claim 9, at least two microchannels (5) are provided on first filter core (21)；
Preferably, 3 microchannels (5) are provided on first filter core (21)；
Preferably, the cross-sectional diameter of the microchannel (5) is 0.2-1mm；
Preferably, bag body (1) internal cavity is divided under volume equal blood bag upper chamber (3) and blood bag by first filter core (21)
Room (4)；
Preferably, in the vertical direction, the ratio of the height of the thickness and bag body (1) of first filter core (21) is 1:7.5-
14；
Preferably, second filter core (22) and third filter core (23) are vertically arranged, and the first filter core (21), the second filter core
(22) it is linked together with third filter core (23)；
Preferably, second filter core (22) and third filter core (23) are arranged along the edge sealing of the bag body (1)；
Preferably, blood transfusion port (6) are provided at the top of the bag body (1)；
Preferably, it is provided with upper hanging hole (7) at the top of the bag body (1), bag body (1) bottom is provided with lower hanging hole (8).
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Application publication date: 20190205 |