CN109298730B - A controllable device for isothermal digestion of tissue samples - Google Patents
A controllable device for isothermal digestion of tissue samples Download PDFInfo
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- CN109298730B CN109298730B CN201811323391.5A CN201811323391A CN109298730B CN 109298730 B CN109298730 B CN 109298730B CN 201811323391 A CN201811323391 A CN 201811323391A CN 109298730 B CN109298730 B CN 109298730B
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- 230000029087 digestion Effects 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 14
- 239000002245 particle Substances 0.000 claims description 23
- 238000010438 heat treatment Methods 0.000 claims description 9
- 230000003287 optical effect Effects 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 6
- 239000007788 liquid Substances 0.000 description 3
- 210000002826 placenta Anatomy 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 102000038379 digestive enzymes Human genes 0.000 description 2
- 108091007734 digestive enzymes Proteins 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N2015/0277—Average size only
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- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a controllable device for a tissue sample constant-temperature digestion process, which comprises a shell, a temperature control device, a universal shaking table device and a sample detection device, wherein a display screen is arranged on the shell, the temperature control device is arranged in the shell and used for controlling the temperature in the shell, the temperature control device displays temperature values on the display screen in real time, the universal shaking table device is arranged in the shell, a sample container is arranged on the universal shaking table device, a tissue sample is placed in the sample container, the universal shaking table device drives the sample container to shake, the sample detection device comprises infrared emission devices and infrared receiving devices which are arranged at two sides of the sample container, infrared light emitted by the infrared emission devices passes through a tissue sample in the sample container and then is converted into an electric signal by the infrared receiving devices, and the electric signal is displayed on the display screen in real time. The invention controls and monitors the digestion process to the greatest extent and ensures the optimal extraction effect.
Description
Technical Field
The invention relates to the technical field of laboratory equipment design, in particular to a controllable device for a constant-temperature digestion process of a tissue sample.
Background
Along with the development of scientific and technological development, people have a deeper and deeper cognition on cells. In the process of separating and extracting primary cells, various digestive enzymes are widely used for digesting and decomposing biological tissue samples from various sources to obtain specific cells, especially in the process of extracting placenta stem cells, such as hematopoietic stem cells or placenta pluripotent stem cells, from placenta, tissue fragments are required to be decomposed in digestive enzyme liquid in a constant temperature device to obtain corresponding target cells;
in the conventional process, the following disadvantages exist:
(1) The higher or lower temperature affects the activity of the enzyme and the activity of the cells;
(2) The rotation speed deviates from the set rotation speed, so that the tissue is too large or too small, and even the cells are directly influenced;
(3) Inconsistencies in digestion time exist;
(4) The digestion degree is difficult to grasp, under manual operation, the error is larger, the difference of the process of digestion is also large, and the process is time-consuming and labor-consuming only by manual visual observation.
Disclosure of Invention
In order to solve the problems of low filtering efficiency, poor effect and the like of the existing mesenchymal stem cells, the invention provides a controllable device for a constant-temperature digestion process of a tissue sample, which comprises the following components:
a housing on which a display screen is provided;
The temperature control device is arranged in the shell and used for controlling the temperature in the shell, and the temperature control device displays the temperature value on the display screen in real time;
the universal shaking table device is arranged in the shell, a sample container is arranged on the universal shaking table device, a tissue sample is placed in the sample container, and the universal shaking table device drives the sample container to shake;
The infrared transmitting device transmits infrared light to the infrared receiving device after passing through the tissue sample in the sample container, and the infrared receiving device converts the received optical signal into an electric signal and displays the electric signal on the display screen in real time.
Preferably, the device further comprises a controller, wherein the temperature control device, the universal shaking table device, the sample detection device and the display screen are all connected with the controller and used for controlling start and stop, and the controller receives temperature data on the temperature control device and receives electrical signals on the infrared receiving device to be converted into particle size information of the tissue sample.
Preferably, a digital display setting button is arranged on the shell and connected with the controller, and the digital display setting button is used for presetting a temperature value of the temperature control device, presetting a working state of the universal shaking table device and presetting the particle size of the tissue sample detected by the sample detection device.
Preferably, the shell is provided with an alarm indicator lamp which is connected with the controller, and when the particle size of the tissue sample obtained by the controller reaches or exceeds a preset range, the alarm indicator lamp is started.
Preferably, the temperature control device comprises a temperature sensor, a heating device and a heat dissipation device which are connected with the controller, and the controller controls the heating device or the heat dissipation device to start according to the temperature value obtained by the temperature sensor.
Preferably, the universal shaking table device adopts a shaking table capable of rotating by 360 degrees.
Preferably, a replaceable base is arranged on the universal shaking table device, and the sample container is a stainless steel container.
Preferably, the infrared emission device and the infrared receiving device are arranged at the bottom of the side face of the sample container.
Preferably, the infrared emission device is arranged on one side of the sample container through a bracket, and the infrared emission device can be adjusted in an up-and-down moving way relative to the bracket.
Compared with the prior art, the invention has the following advantages and positive effects due to the adoption of the technical scheme:
the infrared emission device continuously emits infrared rays, penetrates through the sample container and the sample therein and then reaches the infrared receiving device, converts optical signals into electric signals through the intensity of the received infrared rays, calculates the diameter of sample particles in the sample container and displays the diameter on the digital display screen in real time. According to the invention, through the arrangement of the sample detection device, a mode of identifying the digestion degree of the sample by means of manual experience in the prior art is replaced, the digestion process of the sample is accurately controlled, the human operation error is reduced, the digestion process is controlled to the greatest extent, and the optimal extraction effect is ensured.
Drawings
The above and other features and advantages of the present invention will be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which:
fig. 1 is a schematic structural diagram of a controllable device for the isothermal digestion process of tissue samples according to the present invention.
Detailed Description
The invention will be described in more detail hereinafter with reference to the accompanying drawings showing embodiments of the invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. In the drawings, the size and relative sizes of layers and regions may be exaggerated for clarity.
Referring to fig. 1, the invention provides a controllable device for a constant temperature digestion process of a tissue sample, which comprises a shell 1, a temperature control device, a universal shaking table device and a sample detection device, wherein a display screen 2 is arranged on the shell 1, the temperature control device is arranged in the shell 1 and is used for controlling the temperature in the shell 1 and displaying the temperature value on the display screen 2 in real time, the universal shaking table device is arranged in the shell 1, a sample container 8 is arranged on the universal shaking table device, the tissue sample is arranged in the sample container 8, the universal shaking table device drives the sample container 8 to shake so as to realize uniform mixing of the sample in the sample container 8, the sample detection device comprises infrared emission devices 13 and infrared receiving devices 9 arranged at two sides of the sample container 8, infrared light emitted by the infrared emission devices 13 is converted into an electric signal by the infrared receiving devices 9 after passing through the tissue sample in the sample container 8, and the infrared receiving devices 9 are used for converting the received optical signal into the diameter of particles and displaying the average diameter of the particles in 1s on the display screen in real time.
The infrared emission device 13 continuously emits infrared rays, penetrates through the sample container 8 and the sample therein and then reaches the infrared receiving device 9, continuously receives optical signals through the infrared receiving device 9, can convert the optical signals through the photoelectric converter in the device, and calculates the diameter of the sample particles in the sample container. Specifically, after the infrared emission device 13 emits infrared rays due to the fact that light propagates along a straight line, projection is left on the infrared receiving device 9 due to the blocking of particles, the size of the corresponding particles can be calculated according to the projection size, and the light signal can be continuously received on the infrared receiving device 9 due to the fact that the light speed is very fast, and the average particle diameter in 1S is displayed on the digital display screen in real time through the converter.
If the average diameter continuously appears for more than 20 times within 30 seconds is lower than a set value, the device is identified as digestion termination, and a digestion ending program of the buzzer alarm device is started;
According to the invention, through the arrangement of the sample detection device, a mode of identifying the digestion degree of the sample by means of manual experience in the prior art is replaced, the digestion process of the sample is accurately controlled, the human operation error is reduced, the digestion process is controlled to the greatest extent, and the optimal extraction effect is ensured.
Moreover, after the sample detection device is closed, the controllable device for the constant-temperature digestion process of the tissue sample can be directly used as a common constant-temperature shaking table, and is suitable for digestion or uniform mixing without the requirement on the size of liquid particles.
In this embodiment, the controllable device for the constant temperature digestion process of the tissue sample further includes a controller, where the temperature control device, the universal cradle device, the sample detection device, and the display screen 2 are all connected to the controller for controlling start and stop, and the controller receives temperature data on the temperature control device, receives electrical signals on the infrared receiving device, converts the electrical signals into particle size information of the tissue sample, and displays the particle size information on the display screen 2 in real time. The controller may be implemented by a control circuit board, a computer, a control chip, or the like, which is not limited herein.
In this embodiment, the temperature control device includes a temperature sensor 5, a heating device 6, and a heat dissipating device connected to the controller, where the controller controls the heating device 6 or the heat dissipating device to start according to the temperature value obtained by the temperature sensor, so as to ensure the constant temperature effect of the housing.
The heating device 6 may be implemented by a heating wire, and the heat dissipating device may be implemented by heat dissipating fans 7 and 15 disposed on the side wall of the housing 1, however, in other embodiments, the heating device 6 and the heat dissipating device may be implemented by other structures, which is not limited herein.
In this embodiment, the universal shaker means is a 360 ° rotatable shaker to ensure adequate mixing of the tissue sample.
Further, a replaceable base 12 is provided on the universal shaking table device 11, and the sample container 8 is fixed on the base 12. The base 12 is a replaceable structure and can be provided with various models so as to adapt to different sample holding devices, such as centrifuge tubes, blue-cap bottles, beakers and other transparent devices, thereby greatly widening the application range.
The sample container 8 can be fixed on the base 12 through the fixing spring 10, so that the sample container is fixed on the base 12 tightly, and is not easy to fall off, specifically, the fixing spring is fixed by deformation of the spring and matched with the base 12 as long as the mounting interval of the fixing spring is smaller than the diameter of the container, further, if the fixing spring is arranged in a movable mode, more container types can be adapted, and of course, the fixing mode of the sample container 8 in other embodiments is not limited to the above, and can be adjusted according to specific situations, and the fixing mode is not limited.
In this embodiment, the infrared emitting device 13 and the infrared receiving device 9 are disposed at the lower end of the side of the sample container 8, and since the tissue sample in the sample container 8 is usually located at the bottom of the sample container 8, the infrared can smoothly pass through the tissue sample by the limitation of the positions of the infrared emitting device 13 and the infrared receiving device 9, so as to ensure accurate measurement of the particle size of the sample.
In this embodiment, the infrared emitting device 13 is disposed at one side of the sample container 8 through a bracket 14, and the infrared emitting device 13 can be adjusted by moving up and down relative to the bracket 14, so as to adjust the height of the infrared emitting device 13 to adapt to different specifications of accommodating tissue sample containers, wherein the infrared emitting device 13 can move up and down within a range of 1-20cm, and can be adjusted according to specific conditions, which is not limited herein.
In this embodiment, a digital display setting button 16 is disposed on the housing, and the digital display setting button 16 is connected with the controller and the display screen 2, and is used for presetting a temperature value of the temperature control device, presetting a working state of the universal shaking table device, and presetting a particle size of a tissue sample detected by the sample detection device.
Specifically, parameters on the display screen 2 are reasonably set by the digital display setting button 16, for example, the rotation speed of the universal shaking table device 11 is set to 1000rpm, the acceptable deviation interval is set to 5%, for example, the temperature in the shell 1 is set to 37.0 ℃, the acceptable deviation interval is set to 2%, for example, the maximum particle size in digestive juice is set to 1mm.
In the embodiment, the shell 1 is provided with an alarm indicator lamp which is connected with the controller, and the alarm indicator lamp has two modes of abnormal alarm, namely, buzzing alarm without operation and stop, and digestion ending alarm, namely, buzzing alarm 10s.
The abnormal alarm is specifically that if the deviation of the actual rotation speed and the temperature exceeds the allowable deviation value in the running process of the equipment, the controller is connected to control the alarm indicator lamp to start alarm, and the alarm is continuously buzzed when the equipment is not operated
The digestion ending alarm is specifically that when the particle size of the tissue sample obtained by the controller exceeds 20 times within 30 seconds and reaches the preset maximum diameter, the alarm indicator lamp starts to alarm.
In this embodiment, the casing 1 is further provided with a power switch, which specifically includes an integral switch 3 (for controlling the display screen 2, the temperature control device, and the air cylinder of the universal shaking table device) of the whole device and an infrared switch 4 for controlling the starting of the sample detection device, so that after the infrared switch 4 is conveniently and independently closed, the device can also be used as a common constant temperature shaking table, and is suitable for digestion or uniform mixing without specific requirements on the size of liquid particles.
In this embodiment, the device is integrally formed of a corrosion-resistant and oxidation-resistant material or may be made of a stainless steel metal material so as to extend the service life.
It will be appreciated by those skilled in the art that the invention can be embodied in many other specific forms without departing from the spirit or scope thereof. Although embodiments of the present invention have been described, it is to be understood that the present invention should not be limited to these embodiments, but that variations and modifications can be made by one skilled in the art within the spirit and scope of the present invention as hereinafter defined in the appended claims.
Claims (9)
1. A controllable device for the isothermal digestion of a tissue sample, comprising:
a housing on which a display screen is provided;
The temperature control device is arranged in the shell and used for controlling the temperature in the shell, and the temperature control device displays the temperature value on the display screen in real time;
the universal shaking table device is arranged in the shell, a sample container is arranged on the universal shaking table device, a tissue sample is placed in the sample container, and the universal shaking table device drives the sample container to shake;
The infrared transmitting device transmits infrared light to the infrared receiving device after passing through the tissue sample in the sample container, the infrared receiving device converts the received optical signal into an electric signal, the diameter of sample particles in the sample container is calculated and displayed on the display screen in real time, the diameter of the display screen is the maximum particle diameter allowed after digestion, and if the average diameter continuously appears for more than 20 times within 30 seconds and is lower than a set value, the digestion is identified.
2. The controllable device for the constant temperature digestion process of tissue samples according to claim 1, further comprising a controller, wherein the temperature control device, the universal cradle device, the sample detection device and the display screen are all connected with the controller for controlling start and stop, and the controller receives temperature data on the temperature control device and receives electrical signals on the infrared receiving device for converting into particle size information of the tissue samples.
3. The controllable device for the constant temperature digestion process of tissue samples according to claim 2, wherein a digital display setting button is arranged on the housing, and the digital display setting button is connected with the controller and is used for presetting a temperature value of the temperature control device, presetting an operating state of the universal shaking table device and presetting a tissue sample particle size detected by the sample detection device.
4. The controllable device for the isothermal digestion process of tissue samples according to claim 2, wherein an alarm indicator is provided on the housing, wherein the alarm indicator is connected to the controller, and wherein the alarm indicator is activated when the particle size of the tissue sample obtained by the controller reaches or exceeds a predetermined range.
5. The controllable device for the isothermal digestion process of tissue samples according to claim 2, wherein the temperature control device comprises a temperature sensor, a heating device and a heat dissipating device which are connected with the controller, and the controller controls the heating device or the heat dissipating device to be started according to the temperature value obtained by the temperature sensor.
6. The device of claim 1, wherein the universal shaker means is a 360 ° rotatable shaker.
7. The device for controlling the constant temperature digestion process of tissue samples according to claim 1, wherein the universal shaker device is provided with a replaceable base, and the sample container is a stainless steel container.
8. The controllable device for the isothermal digestion of tissue samples according to claim 1, wherein the infrared emitting device and the infrared receiving device are disposed at the bottom of the sides of the sample container.
9. The apparatus of claim 1, wherein the infrared emitting device is disposed on one side of the sample container by a bracket, and wherein the infrared emitting device is movable and adjustable up and down relative to the bracket.
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CN201811323391.5A CN109298730B (en) | 2018-11-07 | 2018-11-07 | A controllable device for isothermal digestion of tissue samples |
JP2019002078U JP3222736U (en) | 2018-11-07 | 2019-06-10 | Control device for tissue sample isothermal digestion process |
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CN201811323391.5A CN109298730B (en) | 2018-11-07 | 2018-11-07 | A controllable device for isothermal digestion of tissue samples |
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CN117229886B (en) * | 2023-09-20 | 2024-11-05 | 成都诺医德医学检验实验室有限公司 | Constant-temperature tissue digestion device and primary cell separation method |
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WO2017221155A1 (en) * | 2016-06-20 | 2017-12-28 | Genesis Technologies Limited | Automated cell processing systems and methods |
CN208953972U (en) * | 2018-11-07 | 2019-06-07 | 上海坤爱生物科技股份有限公司 | A kind of controllable device of tissue sample constant temperature digestion process |
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CN102428377B (en) * | 2009-05-12 | 2015-08-12 | 赛默飞世尔科技有限公司 | The calibration of particle detection and sensor |
CA2798117C (en) * | 2010-04-30 | 2020-01-14 | Bio-Response Solutions, Inc. | Tissue digestion method and apparatus |
JP2014209090A (en) * | 2013-03-29 | 2014-11-06 | シスメックス株式会社 | Cell analyzing method and cell analyzer |
CN104988118A (en) * | 2015-07-08 | 2015-10-21 | 深圳爱生再生医学科技有限公司 | Method for preparing umbilical cord mesenchymal stem cells |
IL298356B2 (en) * | 2015-11-06 | 2024-09-01 | Ventana Med Syst Inc | Representative diagnoses |
CN107782643A (en) * | 2017-09-27 | 2018-03-09 | 华中科技大学 | A kind of optical fiber dynamic light scattering detection method of high concentration particle group |
CN108486043B (en) * | 2018-02-02 | 2019-03-19 | 中南大学湘雅医院 | Cartilage tissue cutting and enzymolysis digestion integrated device and cartilage tissue treatment method |
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WO2017221155A1 (en) * | 2016-06-20 | 2017-12-28 | Genesis Technologies Limited | Automated cell processing systems and methods |
CN208953972U (en) * | 2018-11-07 | 2019-06-07 | 上海坤爱生物科技股份有限公司 | A kind of controllable device of tissue sample constant temperature digestion process |
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