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CN109266521A - Pneumatic type multichannel microbiological culture media prepares packing instrument automatically - Google Patents

Pneumatic type multichannel microbiological culture media prepares packing instrument automatically Download PDF

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Publication number
CN109266521A
CN109266521A CN201811089635.8A CN201811089635A CN109266521A CN 109266521 A CN109266521 A CN 109266521A CN 201811089635 A CN201811089635 A CN 201811089635A CN 109266521 A CN109266521 A CN 109266521A
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CN
China
Prior art keywords
water valve
electromagnetic water
aluminium sheet
lead screw
microbiological culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811089635.8A
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Chinese (zh)
Inventor
王春利
赵晓娟
王振玉
孙玉慧
孙倩
徐正华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inspection And Quarantine Technology Center Of Jiangmen Entry Exit Inspection And Quarantine Bureau
Original Assignee
Inspection And Quarantine Technology Center Of Jiangmen Entry Exit Inspection And Quarantine Bureau
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Publication date
Application filed by Inspection And Quarantine Technology Center Of Jiangmen Entry Exit Inspection And Quarantine Bureau filed Critical Inspection And Quarantine Technology Center Of Jiangmen Entry Exit Inspection And Quarantine Bureau
Priority to CN201811089635.8A priority Critical patent/CN109266521A/en
Publication of CN109266521A publication Critical patent/CN109266521A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/40Manifolds; Distribution pieces

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of pneumatic type multichannel microbiological culture medias to prepare packing instrument automatically, comprising: aluminium sheet;The first electromagnetic water valve is provided on aluminium sheet;Reagent bottle is provided on heating magnetic stirring apparatus;First electromagnetic water valve is connected with the second electromagnetic water valve and third electromagnetic water valve;Second electromagnetic water valve and third electromagnetic water valve are set to the both ends of portal frame crossbeam;Multiple shunting valves are provided between second electromagnetic water valve and third electromagnetic water valve;The lower end of each shunting valve is provided with test tube, and test tube is set on rack for test tube, and rack for test tube is set on aluminium sheet.The automation of microbiological culture media preparation packing process, rapid, easy can be achieved in the present invention, working efficiency can not only be significantly improved, reduce laboratory human cost, researcher is allowed to free from uninteresting duplicate work, human operational error can be more reduced, guarantees the reliability of culture medium quality, stability and continuity by the gross.

Description

Pneumatic type multichannel microbiological culture media prepares packing instrument automatically
Technical field
The present invention relates to medical equipment technical fields, specifically, in particular to a kind of pneumatic type multichannel microorganism training Feeding base prepares packing instrument automatically.
Background technique
Culture medium is health research, medicine production, clinical bacteria is examined and the important foundation of biological products manufacture.Its quality Quality plays a crucial role the correctness of the growth of microorganism, separation, identification and inspection result, is all kinds of micro- The core of biological culture.The basic program of traditional culture medium preparation be calculate, weigh, dissolving, inverted plate or test tube, go out It is bacterium, steriling test, spare.Involved underlying instrument has high pressure steam sterilizer, electrically heated drying cabinet, electric furnace, balance, amount Cylinder, funnel, test tube, culture dish, beaker and conical flask etc..Process is cumbersome, the amount of labour is big, low efficiency, difficult when high-volume is prepared To guarantee the continuous of quality and stablize.
Occur microbiological culture media dispensing apparatus in recent years, improves confecting efficiency to a certain extent.But it still deposits In more deficiency: 1. it is expensive to prepare dispensing apparatus for commercially available microbiological culture media, about differs from hundreds of thousands to up to a million;② Commercially available microbiological culture media prepare dispensing apparatus main component be peristaltic pump or reciprocating pump, although accurate, be easy to by The corrosion of the substances such as the protein, sugar, starch, the salt that are rich in into culture medium, especially blockage problem are serious, plant maintenance requirement Height, accessory maintenance replacement cost are higher;3. currently, microbiological culture media dispensing apparatus mostly uses greatly single channel (maximum binary channels) Peristaltic pump is only capable of that a sample (binary channels can add two samples) is added every time, limits the separate-loading packaging of culture medium;
Summary of the invention
In order to solve problems in the prior art, the embodiment of the invention provides a kind of pneumatic type multichannel microbiological culture medias Automatic preparation packing instrument.The technical solution is as follows:
On the one hand, it provides a kind of pneumatic type multichannel microbiological culture media and prepares packing instrument, aluminium sheet, heating magnetic force automatically Blender, portal frame, movement mechanism;
The first electromagnetic water valve is provided on the aluminium sheet;Reagent bottle is provided on the heating magnetic stirring apparatus;
First electromagnetic water valve is connected with the second electromagnetic water valve and third electromagnetic water valve;
Second electromagnetic water valve and third electromagnetic water valve are set to the both ends of the portal frame crossbeam;Second electromagnetism Multiple shunting valves are provided between water valve and the third electromagnetic water valve;The lower end of each shunting valve is provided with Test tube, the test tube are set on rack for test tube, and the rack for test tube is set on the aluminium sheet;
The movement mechanism is set to the two sides of the aluminium sheet;Two supporting legs of the portal frame are set to movement mechanism On, and it is mobile by the movement mechanism.
Further, the movement mechanism includes the first lead screw, servo motor, the second lead screw;First lead screw and institute State the two sides that the second lead screw is respectively arranged at the aluminium sheet;
Wherein, the both ends of first lead screw are provided with bearing, and are set on the aluminium sheet by bearing block, described The bearing block is run through in wherein one end of first lead screw, is connect by shaft coupling with the servo motor, and the servo motor is logical Motor cabinet is crossed to be set on the aluminium sheet;
The both ends of second lead screw are provided with linear bearing, and are fixed on the aluminium sheet by bearing block.
Further, two supporting legs of the portal frame are sheathed on respectively on first lead screw and the second lead screw, and It is fixed by feed screw nut.
Further, it is additionally provided with support rod on the aluminium sheet, bracket is provided on the support rod, the bracket is used for Place batcher.
Further, the upper end import of the reagent bottle is connected with nitrogen cylinder;The reagent bottle lower end outlet with First electromagnetic water valve connection, first electromagnetic water valve pass through again the bottom end of three-way valve pipe fitting and the batcher into Mouth is connected, and the upper end outlet of the batcher leads to again after being connected respectively with second electromagnetic water valve, third electromagnetic water valve Six-way valve pipe fitting is crossed to be connected with the culture medium filler on the portal frame.
Technical solution provided in an embodiment of the present invention has the benefit that
The automation of microbiological culture media preparation packing process, rapid, easy can be achieved in the present invention, can not only be significant Working efficiency is improved, laboratory human cost is reduced, allows researcher to free from uninteresting duplicate work, can more subtract Few human operational error, guarantees the reliability of culture medium quality, stability and continuity by the gross.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other Attached drawing.
Fig. 1 is that a kind of pneumatic type multichannel microbiological culture media of the embodiment of the present invention prepares packing instrument schematic diagram automatically;
Fig. 2 is that a kind of pneumatic type multichannel microbiological culture media of the embodiment of the present invention prepares packing instrument top view automatically;
Fig. 3 is that a kind of pneumatic type multichannel microbiological culture media of the embodiment of the present invention prepares packing instrument side view automatically;
Fig. 4 be a kind of pneumatic type multichannel microbiological culture media of the embodiment of the present invention prepare automatically packing instrument module show It is intended to.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to embodiment party of the present invention Formula is described in further detail.
The present invention provides a kind of pneumatic type multichannel microbiological culture medias to prepare packing instrument automatically, referring to Fig. 1-3, packet It includes: aluminium sheet 17, heating magnetic stirring apparatus 8, portal frame 19, movement mechanism;
The first electromagnetic water valve 41 is provided on the aluminium sheet 17;It is provided with reagent bottle 7 on the heating magnetic stirring apparatus 8, Outlet 22 is provided on the reagent bottle 7, the outlet 22 is connect with first electromagnetic water valve 41;
First electromagnetic water valve 41 is connected with the second electromagnetic water valve 42 and third electromagnetic water valve 4;
Second electromagnetic water valve 42 and third electromagnetic water valve 4 are set to the both ends of the portal frame crossbeam;Described second Multiple shunting valves 2 are provided between electromagnetic water valve 42 and the third electromagnetic water valve 4;Each shunting valve 2 Lower end is provided with test tube 3, and the test tube 3 is set on rack for test tube 10, and the rack for test tube 10 is set on the aluminium sheet 17;
The movement mechanism is set to the two sides of the aluminium sheet;Two supporting legs of the portal frame are set to movement mechanism On, and it is mobile by the movement mechanism.
Further, the movement mechanism includes the first lead screw 15, servo motor 12, the second lead screw 21;Described first Thick stick 15 and second lead screw 21 are respectively arranged at the two sides of the aluminium sheet 17;
Wherein, the both ends of first lead screw 15 are provided with bearing 18, and are set to the aluminium sheet by bearing block 16 On 17, the bearing block is run through in wherein one end of first lead screw 15, is connect by shaft coupling 13 with the servo motor 12, The servo motor 12 is set on the aluminium sheet 17 by motor cabinet 14;
The both ends of second lead screw 21 are provided with linear bearing, and are fixed on the aluminium sheet 17 by bearing block.
Further, two supporting legs of the portal frame 19 are sheathed on respectively on first lead screw and the second lead screw, And it is fixed by feed screw nut 1.
Further, it is additionally provided with support rod 11 on the aluminium sheet 17, bracket 5 is provided on the support rod 11, it is described Bracket is for placing batcher 6.
Further, the upper end import of the reagent bottle 7 is connected with nitrogen cylinder;The lower end of the reagent bottle 7 exports 22 connect with first electromagnetic water valve 41, and first electromagnetic water valve 41 passes through three-way valve pipe fitting and the batcher again 6 bottom end import is connected, the upper end outlet of the batcher 6 respectively with second electromagnetic water valve 42, third electromagnetic water valve 4 It is connected again by six-way valve pipe fitting with the culture medium filler on the portal frame 19 after being connected.
In the present embodiment, pneumatic type multichannel microbiological culture media prepares packing instrument automatically and is additionally provided on electronic scale 23.
In the present embodiment, the module signal that pneumatic type multichannel microbiological culture media prepares packing instrument automatically is additionally provided Figure, referring to fig. 4;Wherein, control system uses PLC control system, and PLC control system is connected with each electromagnetic water valve.
Specifically, PLC control system principle:
There are three 12V electromagnetic water valve and 1 7.2V stepper motors altogether
1. power supply is connected, the first magnetic water valve is initially powered up 2min first, and then magnetic water valve 1 is closed;
2. when 2min, the second magnetic water valve and third magnetic water valve are initially powered up 1min, then the second magnetic water valve and third magnetic Water valve is closed;
3. when 3min, stepper motor is initially powered up 3s, it is then shut off;
4. repeating 1. 2. 3. three movements, it is repeated 2 times, it is then out of service, until next secondary source is again switched on.
In the present embodiment, self-made medium preparation packing instrument technical parameter, self-control microbiological culture media preparation are additionally provided Packing instrument technical parameter see the table below 1:
1 technical parameter of table
In the present embodiment, the user that a kind of pneumatic type multichannel microbiological culture media prepares packing instrument automatically is additionally provided Method, specific as follows:
1. the upper end import of reagent bottle 7 is connected with nitrogen cylinder.
2. the lower end outlet of reagent bottle 7 is connected with electromagnetic water valve, electromagnetic water valve passes through three-way valve pipe fitting and 2 again The bottom end import of batcher 6 is connected.
The upper end outlet of 3.2 batchers 6 passes through six-way valve pipe fitting and dragon after being connected respectively with electromagnetic water valve again 5 culture medium fillers on door frame 19 are connected.
4. rack for test tube is total to put 40 test tubes, using it is preceding first calculate 40 test tubes of filling needed for culture volume V, The amount of various reagents required for preparing culture medium is calculated according to the total volume meter of V+20ml.
5.PLC control system is connected with each electromagnetic water valve.
6. preparing culture medium in reagent bottle 7, batcher 6 is arranged according to the culture volume that 5 test tubes of filling need, Nitrogen cylinder second level output pressure is adjusted to 0.2 ± 0.02MPa, PLC control system is opened and sets power supply, pressed after stable gas pressure Lower PLC control system starts to switch, and starts culture medium filling.After filling full 10 test tubes every time, servo motor 12 can be controlled Portal frame 19 is automatically moved to next row test tube and carries out culture medium filling, until 40 test tubes are all filled it up with.
Technical solution provided in an embodiment of the present invention has the benefit that
The automation of microbiological culture media preparation packing process, rapid, easy can be achieved in the present invention, can not only be significant Working efficiency is improved, laboratory human cost is reduced, allows researcher to free from uninteresting duplicate work, can more subtract Few human operational error, guarantees the reliability of culture medium quality, stability and continuity by the gross.
The foregoing is merely a prefered embodiment of the invention, is not intended to limit the invention, all in the spirit and principles in the present invention Within, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.

Claims (5)

1. a kind of pneumatic type multichannel microbiological culture media prepares packing instrument automatically characterized by comprising aluminium sheet, heating magnetic Power blender, portal frame, movement mechanism;
The first electromagnetic water valve is provided on the aluminium sheet;Reagent bottle is provided on the heating magnetic stirring apparatus;
First electromagnetic water valve is connected with the second electromagnetic water valve and third electromagnetic water valve;
Second electromagnetic water valve and third electromagnetic water valve are set to the both ends of the portal frame crossbeam;Second electromagnetic water valve Multiple shunting valves are provided between the third electromagnetic water valve;The lower end of each shunting valve is provided with examination Pipe, the test tube are set on rack for test tube, and the rack for test tube is set on the aluminium sheet;
The movement mechanism is set to the two sides of the aluminium sheet;Two supporting legs of the portal frame are set on movement mechanism, And it is mobile by the movement mechanism.
2. pneumatic type multichannel microbiological culture media as described in claim 1 prepares packing instrument automatically, which is characterized in that described Movement mechanism includes the first lead screw, servo motor, the second lead screw;First lead screw and second lead screw are respectively arranged at institute State the two sides of aluminium sheet;
Wherein, the both ends of first lead screw are provided with bearing, and are set on the aluminium sheet by bearing block, described first The bearing is run through in wherein one end of lead screw, is connect by shaft coupling with the servo motor, the servo motor passes through motor Seat is set on the aluminium sheet;
The both ends of second lead screw are provided with linear bearing, and are fixed on the aluminium sheet by bearing block.
3. pneumatic type multichannel microbiological culture media as claimed in claim 2 prepares packing instrument automatically, which is characterized in that described Two supporting legs of portal frame are sheathed on respectively on first lead screw and the second lead screw, and are fixed by feed screw nut.
4. pneumatic type multichannel microbiological culture media as described in claim 1 prepares packing instrument automatically, which is characterized in that described It is additionally provided with support rod on aluminium sheet, bracket is provided on the support rod, the bracket is for placing batcher.
5. pneumatic type multichannel microbiological culture media as described in claim 1 prepares packing instrument automatically, which is characterized in that described The upper end import of reagent bottle is connected with nitrogen cylinder;The lower end outlet of the reagent bottle is connect with first electromagnetic water valve, First electromagnetic water valve passes through three-way valve pipe fitting again and is connected with the bottom end import of the batcher, the batcher Upper end outlet be connected respectively with second electromagnetic water valve, third electromagnetic water valve after again by six-way valve pipe fitting with it is described Culture medium filler on portal frame is connected.
CN201811089635.8A 2018-09-18 2018-09-18 Pneumatic type multichannel microbiological culture media prepares packing instrument automatically Pending CN109266521A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811089635.8A CN109266521A (en) 2018-09-18 2018-09-18 Pneumatic type multichannel microbiological culture media prepares packing instrument automatically

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Application Number Priority Date Filing Date Title
CN201811089635.8A CN109266521A (en) 2018-09-18 2018-09-18 Pneumatic type multichannel microbiological culture media prepares packing instrument automatically

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101007997A (en) * 2007-01-04 2007-08-01 王书堂 Axial center aeration and jet-booster type stirring fermenter
CN201962273U (en) * 2010-12-08 2011-09-07 中国科学院成都生物研究所 Laboratory culture medium packaging machine
CN102460177A (en) * 2009-05-15 2012-05-16 生物梅里埃有限公司 System and method for automatically venting and sampling a culture specimen container
US20170029760A1 (en) * 2013-12-31 2017-02-02 Gang Niu Full-automatic microorganism detecting enrichment system and enrichment method thereof
CN106978329A (en) * 2017-05-23 2017-07-25 山东省农业科学院玉米研究所 Test tube culture medium automatic sub-packaging device
CN108405026A (en) * 2018-05-29 2018-08-17 珠海丰炎科技有限公司 A kind of rack for test tube of culture medium draft machine
US20180246132A1 (en) * 2015-08-28 2018-08-30 Hitachi High-Technologies Corporation Automatic analyzer and reagent bottle loading method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101007997A (en) * 2007-01-04 2007-08-01 王书堂 Axial center aeration and jet-booster type stirring fermenter
CN102460177A (en) * 2009-05-15 2012-05-16 生物梅里埃有限公司 System and method for automatically venting and sampling a culture specimen container
CN201962273U (en) * 2010-12-08 2011-09-07 中国科学院成都生物研究所 Laboratory culture medium packaging machine
US20170029760A1 (en) * 2013-12-31 2017-02-02 Gang Niu Full-automatic microorganism detecting enrichment system and enrichment method thereof
US20180246132A1 (en) * 2015-08-28 2018-08-30 Hitachi High-Technologies Corporation Automatic analyzer and reagent bottle loading method
CN106978329A (en) * 2017-05-23 2017-07-25 山东省农业科学院玉米研究所 Test tube culture medium automatic sub-packaging device
CN108405026A (en) * 2018-05-29 2018-08-17 珠海丰炎科技有限公司 A kind of rack for test tube of culture medium draft machine

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Application publication date: 20190125