CN109238811B - Full-automatic tissue cell staining system - Google Patents
Full-automatic tissue cell staining system Download PDFInfo
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- CN109238811B CN109238811B CN201811130250.1A CN201811130250A CN109238811B CN 109238811 B CN109238811 B CN 109238811B CN 201811130250 A CN201811130250 A CN 201811130250A CN 109238811 B CN109238811 B CN 109238811B
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- 238000010186 staining Methods 0.000 title claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 54
- 238000005070 sampling Methods 0.000 claims abstract description 54
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 23
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 9
- 238000004043 dyeing Methods 0.000 claims abstract description 8
- 238000002347 injection Methods 0.000 claims abstract description 7
- 239000007924 injection Substances 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims abstract description 6
- 230000005540 biological transmission Effects 0.000 claims description 15
- 238000002955 isolation Methods 0.000 claims description 11
- 238000003825 pressing Methods 0.000 claims description 7
- 239000004809 Teflon Substances 0.000 claims description 3
- 229920006362 Teflon® Polymers 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 abstract description 12
- 238000002474 experimental method Methods 0.000 abstract description 6
- 230000001575 pathological effect Effects 0.000 abstract description 5
- 238000013461 design Methods 0.000 abstract description 4
- 238000012864 cross contamination Methods 0.000 abstract description 3
- 239000012472 biological sample Substances 0.000 abstract description 2
- 230000000642 iatrogenic effect Effects 0.000 abstract description 2
- 230000001953 sensory effect Effects 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 13
- 210000001503 joint Anatomy 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000001744 histochemical effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000592 Artificial Cell Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 238000010882 preoperative diagnosis Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a full-automatic tissue cell staining system, and belongs to the field of medical equipment and instruments. The device consists of a whole machine chassis, a multi-channel pipeline selection sliding table, a power device, a reaction device and a control system. The invention adopts the control of the liquid crystal screen and the control system to each electric control device, realizes the full-automatic dyeing of pathological tissue slices, and realizes the automatic on-off of a liquid path through the cooperation of the peristaltic pump, the diaphragm water pump and the electromagnetic valve; the photoelectric positioning plate, the photoelectric sensor and the sliding sampling device are matched, so that the sampling joint and the sampling hole site are accurately aligned; the sampling joint is accurately abutted with the reagent sample injection hole site through the control of the steering engine; the invention has reasonable design and simple structure, improves the experiment speed and the reagent use efficiency, avoids the cross contamination of the reagent, avoids the bad sensory stimulation of the short-distance contact biological sample and the chemical reagent of the detection personnel, avoids the possible iatrogenic pollution and improves the biological safety level of the laboratory.
Description
Technical Field
The invention relates to an immunocytochemistry staining technology, in particular to a full-automatic tissue cell staining system. Belongs to the field of medical equipment and instruments.
Background
The immunocytohistochemical staining is to utilize the characteristic of the specific combination of known antibody and cell antigen, to make the display agent marked on the antibody display certain color through chemical reaction, and to observe with microscope, fluorescence microscope or electron microscope, so as to achieve the aim of quantitative and positioning analysis of chemical components in tissue and cell structure. This technology has now been applied in great numbers to clinical work and scientific research. In clinical aspects, the pathological examination and the operation pathological examination are mainly carried out. The purpose of pathological examination of operation is to definitely diagnose and verify the preoperative diagnosis, and improve the clinical diagnosis level; and secondly, after the diagnosis is clear, the next treatment scheme and estimated prognosis can be determined, so that the clinical treatment level is improved. A great amount of valuable scientific research data can be obtained through clinical pathological analysis.
However, the immune cell histochemical staining technique is a multi-step and multi-factor determined experimental method, and has many interference factors, even an undesirable staining result can occur, so that judgment errors are easily caused. The traditional artificial cell histochemical staining is to put the tissue cell slice pretreated by the steps of baking slice, dewaxing, air drying and the like into a reaction box (staining jar) with different reagents in sequence for staining. Because of the wide variety of reagents used, the reaction time of each step is also critical, requiring a skilled artisan to perform very careful procedures. The artificial dyeing is not only easy to cause the failure of dyeing the cell tissues due to incorrect operation sequence or inaccurate time control, but also the reagent used in part of the steps is harmful to people, and the safety accident is easy to occur due to incorrect operation.
Automatic dyeing equipment currently popular in the market has: such as Chinese patent publication No.: CN206450486U, publication date: 2017-08-29, invention name: an automatic smear dyeing machine, which is provided with a triaxial structure device; such as Chinese patent publication No.: CN103558074B, publication date: 2016-04-13, name of invention: a cell staining device, a transmission type structural device; such as Chinese patent publication No.: CN206038393U, publication date: 2017-03-22, name: a cell staining pelleter is provided with a turntable type structural device. However, the above-mentioned methods have many problems such as low reagent utilization efficiency, long time for performing staining experiments, small number of samples that can be simultaneously processed, and cross contamination caused by various reagents.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a full-automatic tissue cell staining system which has the advantages of simple structure, convenient and quick operation and low manufacturing cost and can quickly stain tissue slices.
The technical scheme adopted for achieving the purposes of the invention is as follows:
A full-automatic tissue cell staining system comprises a complete machine chassis, a multi-channel pipeline selection slipway, a power device, a reaction device and a control system. The multi-channel pipeline selection sliding table consists of a bench, a reagent joint plate and a sliding sampling device; the reagent joint plate is fixed on the front half part above the rack; the reagent joint plate is provided with a sampling Kong Weizu, and all hole sites in the sampling hole group are arranged in parallel along the length direction; the lower ends of the sampling hole groups are respectively connected with hoses; the rubber ring pressing plate is provided with hole positions corresponding to the sampling hole position groups, and the rubber ring pressing plate covers the upper parts of the sampling hole position groups; the sliding sampling device comprises a sliding platform, a sampling module and a photoelectric positioning plate; the sliding platform is fixed on the rear half part above the rack and is parallel to the sampling Kong Weizu; the sampling module comprises a horizontal sliding block, a steering engine transmission block and a vertical sliding block; the horizontal sliding block is movably arranged in a track above the sliding platform through a belt pulley transmission device; the steering engine is fixed above the horizontal sliding block through a bracket formed by a horizontal fixing plate and a vertical fixing plate, and a steering engine arm of the steering engine is movably embedded in a sliding groove in a steering engine transmission block; the vertical sliding block is fixed under the steering engine transmission block, and a sampling joint is arranged under the vertical sliding block; the axis of the sampling joint and the axis of the hole in the sample hole group are positioned on the same plane in the vertical direction; the photoelectric positioning plate is vertically fixed on the rear side of the sliding platform and is parallel to the sliding platform; a group of strip-shaped notches are engraved above the photoelectric positioning plate, and each strip-shaped notch corresponds to each hole site in the sample injection hole group; a photoelectric sensor is arranged below the rear end of the horizontal sliding block, and the position of a detection gap inside the photoelectric sensor is matched with the upper edge of the photoelectric positioning plate; one end of the sliding platform is provided with a zero sensor, and the other end of the sliding platform is provided with a limit switch.
The reaction device comprises a reaction box cover and a reaction box body; a water receiving disc is arranged below the reaction box body, and liquid path connectors are respectively arranged at the upper part and the lower part of the reaction box body; the water receiving disc is fixed above the isolation bracket; the reaction box cover and the reaction box body are both made of Teflon materials.
The power device comprises a peristaltic pump and a diaphragm water pump; the peristaltic pump and the diaphragm water pump are fixed on the chassis of the whole machine; one end of the peristaltic pump is connected with the liquid outlet of the sampling joint through a hose, the other end of the peristaltic pump is connected with the liquid path joint at the lower part of the reaction box body through a hose, and an electromagnetic valve is arranged between the hoses; the liquid inlet of the diaphragm water pump is connected with the water taking joint through a hose, and the liquid outlet of the diaphragm water pump is connected with the liquid path joint at the upper part of the reaction box body through a hose.
The control system comprises a liquid crystal screen, a power supply module and a control circuit board; the power supply module, the motor driving module and the control circuit board are all fixed on the isolation bracket; the isolation bracket is fixed above the chassis of the whole machine and isolates the system circuit part from the rack; the liquid crystal screen, the power supply module, the motor driving module, the steering engine, the photoelectric sensor, the limit switch, the diaphragm water pump, the zero position sensor and the electromagnetic valve are all connected with the control circuit board through wires; peristaltic pump and sliding platform driving motor all are connected with motor drive module through the wire.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the beneficial effects and advantages that:
According to the invention, the peristaltic pump, the diaphragm water pump and the electromagnetic valve are matched, so that the automatic on-off of the liquid path is realized; the photoelectric positioning plate, the photoelectric sensor and the sliding sampling device are matched, so that the sampling joint and the sampling hole site are accurately aligned; the sampling joint is accurately abutted with the reagent sample injection hole site through the control of the steering engine; the sliding block is ensured to move in the safety range of the sliding platform through the limit switch and the photoelectric sensor; the invention adopts the structural design of the multi-channel pipeline selection sliding table, so that the cross contamination of reagents is avoided; due to the adoption of the design of a single reaction device, the dosage of the reagent is effectively reduced, and the use efficiency of the reagent is improved; the specially-made paired reaction box cover and reaction box body are adopted, so that the resistance of the reaction device to corrosive reagents is realized, and the number of samples processed simultaneously (100 samples are supported at the maximum) is effectively increased; the liquid crystal screen and the control system are adopted to control all the electric control equipment, so that the full-automatic dyeing of pathological tissue slices is realized; the full-automatic tissue cell staining system has reasonable design and simple structure, can greatly improve the speed of inspection and experiment, effectively avoids bad sensory stimulation of a detector in close contact with biological samples and chemical reagents, effectively avoids possible iatrogenic pollution, and improves the biological safety level of a laboratory.
Drawings
FIG. 1 is a schematic diagram of the three-dimensional structure of a fully automated tissue cell staining system according to the present invention.
Fig. 2 is a schematic perspective view of fig. 1 rotated 180 °.
Detailed Description
The invention is described in further detail below with reference to the accompanying drawings.
A full-automatic tissue cell staining system comprises a complete machine chassis 12, a multi-pipeline selection slipway, a power device, a reaction device and a control system.
The multichannel pipeline selection slipway consists of a rack 17, a reagent joint plate 18 and a sliding sampling device. The reagent joint plate 18 is fixed on the upper front half part of the rack 17; the reagent joint plate 18 is provided with a sampling Kong Weizu, the sampling hole group 20 comprises 10 sampling hole positions with the same size, and all the hole positions are arranged in parallel along the length direction; the lower ends of the sampling hole group 20 are respectively connected with hoses; the rubber ring pressing plate 19 is provided with hole positions corresponding to the sampling Kong Weizu, and the rubber ring pressing plate 19 covers the upper part of the sampling hole group 20. The sliding sampling device comprises a sliding platform 15, a sampling module and a photoelectric positioning plate 5; the slide platform 15 is fixed to the upper rear half of the rack 17 adjacent to the reagent tab plate, and the slide platform 15 is parallel to the sample well array 20. The sampling module comprises a horizontal sliding block 3, a steering engine 2, a steering engine transmission block 1 and a vertical sliding block 22; the horizontal sliding block 3 is driven by a belt pulley transmission device to move in a track above the sliding platform 15; the steering engine 2 is fixed above the horizontal sliding block 3 through a bracket formed by a horizontal fixing plate and a vertical fixing plate, and a steering engine arm of the steering engine 2 is movably embedded in a sliding groove in the steering engine transmission block 1; the vertical sliding block 22 is fixed under the steering engine transmission block 1, and a sampling joint 21 is arranged under the vertical sliding block 22; the axis of the sampling joint 21 and the axis of the hole in the sample hole group 20 are positioned on the same plane in the vertical direction; the rudder arm drives the vertical sliding block 22 to move up and down, so that the sampling joint 21 is in butt joint with any hole site in the sampling Kong Weizu; the photoelectric positioning plate 5 is vertically fixed on the rear side of the sliding platform 15, and the photoelectric positioning plate 5 is parallel to the sliding platform 15; a group of strip-shaped notches are engraved above the photoelectric positioning plate 5, and each strip-shaped notch corresponds to each hole site in the sample injection hole group 20. A photoelectric sensor 4 is arranged below the rear end of the horizontal sliding block 3, and the position of a detection gap inside the photoelectric sensor 4 is matched with the upper edge of the photoelectric positioning plate 5; when the detection gap in the photoelectric sensor 4 passes through the strip-shaped gap, the internal photoelectric signal is changed and transmitted to the control system, and the system positions the sampling module in the horizontal direction; one end of the sliding platform 15 is provided with a zero sensor 23, and the other end is provided with a limit switch 14, so that the horizontal sliding block 3 can be ensured to move in a proper range.
The reaction device comprises a reaction box cover 6 and a reaction box body 7. A water receiving disc 8 is arranged below the reaction box body 7, and liquid path connectors are respectively arranged at the upper part and the lower part of the reaction box body 7; the water receiving disc 8 is fixed above the isolation bracket 9, so that liquid in the reaction device can be prevented from flowing into the system; the reaction box cover 6 and the reaction box body 7 are made of Teflon materials, so that the acid or alkaline reagent can be effectively prevented from corroding the box body.
The power unit comprises a peristaltic pump 13 and a diaphragm pump 16. The peristaltic pump 13 and the diaphragm water pump 16 are fixed on the whole machine chassis 12; one end of the peristaltic pump 13 is connected with a liquid outlet of the sampling connector 21 through a hose, the other end of the peristaltic pump 13 is connected with a liquid path connector at the lower part of the reaction box body 7 through a hose, the peristaltic pump 13 rotates clockwise to pump liquid in the reaction device, and the peristaltic pump 13 rotates anticlockwise to pump external reagent into the reaction device; an electromagnetic valve 24 is arranged between the hoses, and the opening and closing of the electromagnetic valve 24 can control the on-off of a liquid path; the liquid inlet of the diaphragm water pump 16 is connected with the water taking connector 26 through a hose, the liquid outlet of the diaphragm water pump 16 is connected with the liquid path connector at the upper part of the reaction box body 7 through a hose, and the diaphragm water pump 16 operates to pump external water into the reaction device.
The control system comprises a liquid crystal screen, a power module 11 and a control circuit board 10. The power supply module 11, the motor driving module 25 and the control circuit board 10 are all fixed on the isolation bracket 9; the isolation bracket 9 is fixed above the chassis 12 of the whole machine, and the isolation bracket 9 isolates the circuit part of the system from the bench 17; the liquid crystal screen, the power supply module 11, the motor driving module 25, the steering engine 2, the photoelectric sensor 4, the limit switch 14, the diaphragm water pump 16, the zero sensor 23 and the electromagnetic valve 24 are all connected with the control circuit board 10 through wires, and the control circuit board 10 is responsible for controlling the electric control equipment; the peristaltic pump 13 and the driving motor of the sliding platform 15 are connected with a motor driving module 25 through wires, and the motor driving module 25 is responsible for driving the relevant motors.
The working principle of the invention is as follows:
Before the experimental procedure starts, the operator needs to put the sample slide to be processed into the reaction device, and the external hose pipe of the system needs to be connected into the corresponding reagent container.
After the system is started, an operator uses a liquid crystal display to set experimental flow steps and corresponding time of each step.
After the experimental process starts, the system drives the whole sampling module to move in the track above the sliding platform 15 through a belt pulley transmission device contained in the sliding platform 15, so that the photoelectric sensor 5 at the rear side of the horizontal sliding block 3 is driven to move at the upper end of the photoelectric positioning plate 5, the detection notch at the inner side of the photoelectric sensor 5 is matched with the upper end of the photoelectric positioning plate 5, 10 strip-shaped notches are engraved at the upper end of the photoelectric positioning plate 5, each time the photoelectric sensor 5 passes through one strip-shaped notch, the photoelectric signal in the photoelectric sensor changes along with the change and is output to the control circuit board 10, the strip-shaped notch corresponds to each sampling hole site in the reagent joint plate 18, and the system positions the sampling module above the corresponding hole site of a required reagent. The reagent tab 18 contains 10 sites, 9 of which are the corresponding sites for the test reagents and 1 of which are the sites for the reaction device to exhaust.
After the positioning is finished, the steering engine 2 drives the vertical sliding block 22 to move in the vertical direction through the steering engine transmission block 1, so that the sampling joint 21 fixed at the lower part of the vertical sliding block 22 is in butt joint with a hole position required in the sampling Kong Weizu, and the sealing property between the joint and the hole position after the butt joint can be ensured by the rubber ring pressing plate 19 above the sampling hole group 20.
After the butt joint is finished, the control circuit board 10 sends out a control signal to enable the electromagnetic valve 24 to be opened, the motor driving module 25 drives the peristaltic pump to rotate, and the peristaltic pump 13 rotates clockwise to pump out liquid in the reaction device, so that waste discharge operation is finished; the peristaltic pump 13 rotates anticlockwise to pump the external reagent into the reaction device, so that the sample injection operation is completed. The solenoid valve 24 is then closed to ensure that the liquid in the reaction apparatus does not flow back into the peristaltic pump 13 due to the water pressure. When the experimental step requires water to participate in the reaction, the control circuit board 10 sends out a control signal to drive the diaphragm water pump 16 to pump external water into the reaction device, and the waste discharge mode of the water is the same as that of the rest reagents. Because the water liquid enters the reaction device from the liquid path joint at the upper part of the reaction box, the system can realize the operation of spraying the water liquid from the upper part of the sample slide.
In the dyeing operation, the reaction box cover 6 is tightly connected with the reaction box body 7, so that the tightness of the reaction device is ensured; the sample slide reacts with the experimental reagent in the device; after the time of the step is set, the system pumps the waste liquid out of the reaction device.
In the experiment, the display screen displays the characteristics of the sample to be processed and the operation flow, so that the operator can observe and operate conveniently.
Claims (2)
1. A full-automatic tissue cell staining system is characterized in that: the dyeing system consists of a complete machine chassis (12), a multi-channel pipeline selection sliding table, a power device, a reaction device and a control system;
The multi-channel pipeline selection sliding table consists of a table frame (17), a reagent joint plate (18) and a sliding sampling device; the reagent joint plate (18) is fixed on the front half part above the rack (17); the reagent joint plate (18) is provided with a sampling Kong Weizu (20), and all hole sites in the sampling Kong Weizu (20) are arranged in parallel along the length direction; the lower ends of the sampling Kong Weizu (20) are respectively connected with a hose; a hole site corresponding to the sample Kong Weizu (20) is formed in the rubber ring pressing plate (19), and the rubber ring pressing plate (19) covers the sample Kong Weizu (20); the sliding sampling device comprises a sliding platform (15), a sampling module and a photoelectric positioning plate (5); the sliding platform (15) is fixed on the rear half part above the rack (17), and the sliding platform (15) is parallel to the sampling Kong Weizu (20); the sampling module comprises a horizontal sliding block (3), a steering engine (2), a steering engine transmission block (1) and a vertical sliding block (22); the horizontal sliding block (3) is movably arranged in a track above the sliding platform (15) through a belt pulley transmission device; the steering engine (2) is fixed above the horizontal sliding block (3) through a bracket formed by a horizontal fixing plate and a vertical fixing plate, and a steering engine arm of the steering engine is movably embedded in a sliding groove in the steering engine transmission block (1); the vertical sliding block (22) is fixed under the steering engine transmission block (1), and a sampling joint (21) is arranged under the vertical sliding block (22); the axis of the sampling joint (21) and the axis of the hole site in the sample injection Kong Weizu (20) are positioned on the same plane in the vertical direction; the photoelectric positioning plate (5) is vertically fixed on the rear side of the sliding platform (15), and the photoelectric positioning plate (5) is parallel to the sliding platform (15); a group of strip-shaped notches are engraved above the photoelectric positioning plate (5), and each strip-shaped notch corresponds to each hole site in the sample injection Kong Weizu (20); a photoelectric sensor (4) is arranged below the rear end of the horizontal sliding block (3), and the position of a detection gap inside the photoelectric sensor (4) is matched with the upper edge of the photoelectric positioning plate (5); one end of the sliding platform (15) is provided with a zero sensor (23), and the other end of the sliding platform is provided with a limit switch (14);
the reaction device comprises a reaction box cover (6) and a reaction box body (7); a water receiving disc (8) is arranged below the reaction box body (7), and liquid path connectors are respectively arranged at the upper part and the lower part of the reaction box body (7); the water receiving disc (8) is fixed above the isolation bracket (9);
The power device comprises a peristaltic pump (13) and a diaphragm water pump (16); the peristaltic pump (13) and the diaphragm water pump (16) are fixed on the chassis (12) of the whole machine; one end of the peristaltic pump (13) is connected with a liquid outlet of the sampling joint (21) through a hose, the other end of the peristaltic pump (13) is connected with a liquid path joint at the lower part of the reaction box body (7) through a hose, and an electromagnetic valve (24) is arranged between the hoses; the liquid inlet of the diaphragm water pump (16) is connected with the water taking joint (26) through a hose, and the liquid outlet of the diaphragm water pump (16) is connected with the liquid path joint at the upper part of the reaction box body (7) through a hose;
The control system comprises a liquid crystal screen, a power supply module (11) and a control circuit board (10); the power supply module (11), the motor driving module (25) and the control circuit board (10) are all fixed on the isolation bracket (9); the isolation bracket (9) is fixed above the chassis (12) of the whole machine, and the isolation bracket (9) isolates the circuit part of the system from the rack (17); the liquid crystal display, the power supply module (11), the motor driving module (25), the steering engine (2), the photoelectric sensor (4), the limit switch (14), the diaphragm water pump (16), the zero sensor (23) and the electromagnetic valve (24) are all connected with the control circuit board (10) through wires; the peristaltic pump (13) and the driving motor of the sliding platform (15) are connected with the motor driving module (25) through wires.
2. A fully automated tissue cell staining system according to claim 1 wherein: the reaction box cover (6) and the reaction box body (7) are made of Teflon materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811130250.1A CN109238811B (en) | 2018-09-27 | 2018-09-27 | Full-automatic tissue cell staining system |
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| CN201811130250.1A CN109238811B (en) | 2018-09-27 | 2018-09-27 | Full-automatic tissue cell staining system |
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| CN109238811B true CN109238811B (en) | 2024-05-10 |
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| CN111983245B (en) * | 2019-05-24 | 2025-01-07 | 南京金斯瑞生物科技有限公司 | Bioreactor and method for biological detection based on the same |
| CN112444436B (en) * | 2020-11-23 | 2022-06-21 | 浙江大学 | Pretreatment method for integral immunostaining of adult diaphragm |
| CN112747989B (en) * | 2020-12-23 | 2023-01-13 | 山东骏腾医疗科技有限公司 | Automatic dyeing box for tissue slice |
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