CN109234176A - Cordyceps sinensis mother culture media and preparation method thereof - Google Patents
Cordyceps sinensis mother culture media and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of cordyceps sinensis mother culture medias, it includes following raw materials according by weight: 0.5-1.5 parts of mulberry silkworm chrysalis powder, 1-3 parts of carrot, 1-3 parts of potato, 0.5-1.5 parts of apple, 0.5-1.5 parts of peptone, 1-3 parts of glucose, 0.05-0.15 parts of potassium dihydrogen phosphate, 0.05-0.15 parts of magnesium sulfate, 0.5-1.5 parts of agar, 0.5-1.5 parts of sorghum, 90-110 parts of water.Preparation method is cut to 1 centimeter or so fritter after including the following steps: 1. carrots and potato and apple peel；2. above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum one are reinstated boiling to open and kept for 1 hour, supernatant is obtained.3. supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar are packed into teat glass after being sufficiently mixed, using 1 hour of high-temperature sterilization, cordyceps sinensis mother culture media can be become after cooling.
Description
Technical field
The present invention relates to cordyceps sinensis, in particular to a kind of cordyceps sinensis mother culture media and preparation method thereof.
Background technique
Cordyceps sinensis is China's second class protection species, is the rare Chinese medicine rarity in China, it is answered in China's folklore
With existing more than 2,000 years history.Cordyceps sinensis is not only the medicinal fungi with good healthcare function, and is with very high
The high-grade nourishing edible fungus of nutritive value, with the raising of people's living standard and quality of life, to functional health product
Demand also expands increasingly.Since wild cordyceps Distribution Area is narrow, natural parasitic rate is low, it is severe to require living environment condition
It carves, so resource itself is than relatively limited.The main place of production ecological environment of cordyceps sinensis is by artificial serious destruction in recent years, largely
Blindly unreasonable excavation causes resource to reduce increasingly, and yield declines year by year.And cordyceps sinensis is due to can be with dual-purpose of drug and food, and people
Its new pharmacological action is constantly discovered, so multiple countries need to double to it, price goes up year by year, and international market is increasingly tight
It lacks.Because wild resource is exhausted year by year, the protection of resources and sustainable use of cordyceps sinensis are always extensive concern inside and outside industry
The Chinese caterpillar fungus bacterial filament powder of focus, the production of artificial fermentation's method has been known and has approved.
Many personal and unit researchs domestic at present and open Chinese caterpillar fungus culture medium and fermentation method for producing, but at present
These technologies mostly use microbial fermentation often to use culture medium, substantially using egg, milk, etc. as fermentation food source and medium component
Selection, although being all so that strain is grown according to microbiological the principle of fermentation, the difference of food source necessarily causes mycelium
Property difference, but the culture medium is had nothing in common with each other, and also height is different for yield and quality, and such case seriously affects its production
Industry development.In the Mother culture of cordyceps sinensis, whether the formula of culture medium is excellent, directly affects mycelial growth.It is produced
Cordyceps sinensis fermented product is variant in chemical component and active material and natural cordyceps, thus cultivates in the prior art
Cordyceps sinensis it is generally existing of poor quality, the lower problem of nutrient composition content.Artificial culture difficulty is big and technical bottleneck is more,
One of technical bottleneck is that cordyceps sinensis parent species cultivate base.
Summary of the invention
It is an object of the present invention to overcome the deficiencies of the prior art and provide a kind of cordyceps sinensis mother culture media and
Preparation method.The present invention passes through the practice of many years, develops a kind of culture medium of suitable cordyceps sinensis parent species growth, the present invention
Cordyceps sinensis mother culture media raw material nutrition according to needed for Cordyceps strain carry out rationally science collocation, pass through addition
The plant nutrients such as apple and carrot, reduce the intake of animal sources, avoid animal virulence factor risk, heavy metal contains
Low, antibiotic-free residual is measured, it is highly-safe.Raw material components and with scientific and reasonable, culture medium is full of nutrition, comprehensive, is entirely capable of full
The growth of sufficient aweto strain and mycelia.The mycelium fermentation period is short, microbiological contamination risk is low, low energy consumption.And raw material sources are wide
It is general, price steadiness, at low cost.Mycelial yield is high, good in economic efficiency.
A kind of cordyceps sinensis mother culture media of the invention, includes following raw materials according by weight: mulberry silkworm chrysalis powder 0.5-
1.5 parts, 1-3 parts of carrot, 1-3 parts of potato, 0.5-1.5 parts of apple, 0.5-1.5 parts of peptone, 1-3 parts of glucose, biphosphate
0.05-0.15 parts of potassium, 0.05-0.15 parts of magnesium sulfate, 0.5-1.5 parts of agar, 0.5-1.5 parts of sorghum, 90-110 parts of water.
It is preferred that its raw material proportioning are as follows: 1 part of mulberry silkworm chrysalis powder, 2 parts of carrot, 2 parts of potato, 1 part of apple, 1 part of peptone, grape
2 parts, 0.1 part of potassium dihydrogen phosphate, 0.1 part of magnesium sulfate, 1 part of agar, 1 part of sorghum, 100 parts of water of sugar.
A kind of preparation method of cordyceps sinensis mother culture media of the present invention comprising following steps:
(1) being cut to 1 centimeter or so fritter after carrot and potato and apple peel；
(2) above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum are added to the water and boil and is kept for 1 hour, obtain supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
It is interior, using 0.5-1.5 hour of high-temperature sterilization, cordyceps sinensis mother culture media can be become after cooling.
It is preferred that the high-temperature sterilization condition of the step (3) is: 120 DEG C of pressure 0.114MPa, temperature.
It is preferred that the high-temperature sterilization time of the step (3) is 1 hour.
The peptone is preferably phytone.
The available primary carbon source substance of cordyceps sinensis is glucose, sucrose, maltose, starch, pectin etc., with monosaccharide or small point
The utilizing status of sub- disaccharide is preferred.Secondly, nitrogen is the essential elements of cordyceps sinensis synthetic proteins matter and nucleic acid substances, cordyceps sinensis can benefit
The type of nitrogen substance especially organic nitrogen is more, such as peptone, beancake powder, yeast extract；If can also be used
Dry inorganic nitrogen, but preferably the utilizing status of organic nitrogen.During Chinese caterpillar fungus hypha and its stroma are grown again, essential member
Element is mineral element；Suitable auxin effective stimulus and the growth for promoting Chinese caterpillar fungus hypha, and its biomass is greatly improved, most
Afterwards, reasonable matrix carbon-nitrogen ratio (CN) is the requirement of artificial cultivation cordyceps sinensis, otherwise, will lead to or mycelia slow growth,
Or seriously polluted or aerial hyphae is excessively prosperous, it is difficult to stroma occur, even if there is stroma differentiation, product quantity and quality are equal
There is different degrees of decline.
Chinese caterpillar fungus culture medium feature provided by the invention is that sorghum can not only provide carbon source, but also sub- oil rich in
Acid, there are also multivitamins, minerals；Potato and dried silkworm chrysalis meal and peptone etc. can provide it to cordyceps sinensis must be enough
Nitrogen source and carbon source, and match rationally.Apple is full of nutrition, and nature and flavor are mild, carbohydrate rich in, vitamin and micro
Element has carbohydrate, organic acid, pectin, protein, calcium, phosphorus, potassium, iron, vitamin A, vitamin B, vitamin C and dietary fiber,
Separately contain malic acid, tartaric acid, carrotene, be in all vegetables and fruits nutritive value closest to perfect one.The present invention pass through by
It is added as raw material, by a large number of experiments show that, by its in raw material carrot and the rational proportions such as potato, can be significantly
Its effect is improved, synergistic effect is played, culture medium culture medium is preferably supplied to and provides sufficient nutrition abundant, promote
The good growth of mycelia.
The utility model has the advantages that present invention nutrition according to needed for Cordyceps strain carries out rationally science collocation, by the way that apple is added
With the plant nutrients such as carrot, reduce the intake of animal sources, avoid animal virulence factor risk, content of beary metal it is low,
Antibiotic-free residual, it is highly-safe.Raw material components and with scientific and reasonable, culture medium is full of nutrition, comprehensive, is able to satisfy winter worm completely
The growth of summer grass strain and mycelia.The mycelium fermentation period is short, microbiological contamination risk is low, low energy consumption.And raw material sources are extensive, price
It is stable, at low cost.Mycelial yield is high, good in economic efficiency.
Specific embodiment
For a better understanding of the present invention, below with reference to embodiment, the invention will be further described, but the present invention claims
The range of protection is not limited to the range of embodiment expression.
Embodiment 1:
A kind of cordyceps sinensis mother culture media of the present embodiment, includes following raw materials according by weight: 1 part of mulberry silkworm chrysalis powder, recklessly
2 parts of radish, 2 parts of potato, 1 part of apple, 1 part of peptone, 2 parts of glucose, 0.1 part of potassium dihydrogen phosphate, 0.1 part of magnesium sulfate, agar 1
Part, 1 part of sorghum, 100 parts of water.
A kind of preparation method of cordyceps sinensis mother culture media described in the present embodiment comprising following steps:
(1) being cut to 1 centimeter or so fritter after carrot and potato and apple peel；
(2) above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum one are reinstated boiling to open and kept for 1 hour, obtains supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
Interior, using 1 hour of high-temperature sterilization, high-temperature sterilization condition is 120 DEG C of pressure 0.114MPa, temperature, can be become after cooling
Cordyceps sinensis mother culture media.
Embodiment 2:
A kind of cordyceps sinensis mother culture media of the present embodiment, includes following raw materials according by weight: 0.5 part of mulberry silkworm chrysalis powder,
1 part of carrot, 3 parts of potato, 0.5 part of apple, 1.5 parts of peptone, 3 parts of glucose, 0.05 part of potassium dihydrogen phosphate, magnesium sulfate 0.15
Part, 0.5 part of agar, 1.5 parts of sorghum, 100 parts of water.
A kind of preparation method of cordyceps sinensis mother culture media described in the present embodiment comprising following steps:
(1) being cut to 1 centimeter or so fritter after carrot and potato and apple peel；
(2) above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum one are reinstated boiling to open and kept for 1 hour, obtains supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
Interior, loading amount is high 1/4 of pipe, and tampon sealing beyond the Great Wall is tied up, and using 1 hour of high-temperature sterilization, high-temperature sterilization condition is pressure
120 DEG C of 0.114MPa, temperature can become cordyceps sinensis mother culture media after cooling.
Embodiment 3:
A kind of cordyceps sinensis mother culture media of the present embodiment, includes following raw materials according by weight: 1.5 parts of mulberry silkworm chrysalis powder,
1 part of carrot, 3 parts of potato, 1.5 parts of apple, 0.5 part of peptone, 3 parts of glucose, 0.15 part of potassium dihydrogen phosphate, magnesium sulfate 0.05
Part, 0.05 part of agar, 0.5 part of sorghum, 90 parts of water.
A kind of preparation method of cordyceps sinensis mother culture media described in the present embodiment comprising following steps:
(1) being cut to 1 centimeter or so fritter after carrot and potato and apple peel；
(2) above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum one are reinstated boiling to open and kept for 1 hour, obtains supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
Interior, loading amount is high 1/4 of pipe, and tampon sealing beyond the Great Wall is tied up, and using 0.5 hour of high-temperature sterilization, high-temperature sterilization condition is pressure
Strong 0.114MPa, 120 DEG C of temperature, can become cordyceps sinensis mother culture media after cooling.
Comparative example 1:
A kind of cordyceps sinensis mother culture media of the present embodiment, includes following raw materials according by weight: 5 parts of mulberry silkworm chrysalis powder, recklessly
1 part of radish, 3 parts of potato, 1.5 parts of apple, 5 parts of peptone, 1 part of glucose, 0.15 part of potassium dihydrogen phosphate, 0.05 part of magnesium sulfate, fine jade
0.05 part of rouge, 5 parts of sorghum, 90 parts of water.
A kind of preparation method of cordyceps sinensis mother culture media described in the present embodiment comprising following steps:
(1) being cut to 1 centimeter or so fritter after carrot and potato and apple peel；
(2) above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum one are reinstated boiling to open and kept for 1 hour, obtains supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
Interior, loading amount is high 1/4 of pipe, and tampon sealing beyond the Great Wall is tied up, and using 0.5 hour of high-temperature sterilization, high-temperature sterilization condition is pressure
Strong 0.114MPa, 120 DEG C of temperature, can become cordyceps sinensis mother culture media after cooling.
Comparative example 2
A kind of cordyceps sinensis mother culture media of the present embodiment, includes following raw materials according by weight:
1 part of mulberry silkworm chrysalis powder, 2 parts of carrot, 2 parts of potato, 1 part of peptone, 2 parts of glucose, 0.1 part of potassium dihydrogen phosphate, magnesium sulfate
0.1 part, 1 part of agar, 1 part of sorghum, 100 parts of water.
A kind of preparation method of cordyceps sinensis mother culture media described in the present embodiment comprising following steps:
(1) being cut to 1 centimeter or so fritter after carrot and potato decortication；
(2) above two block and mulberry silkworm chrysalis powder and sorghum one are reinstated boiling to open and kept for 1 hour, obtains supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
Interior, loading amount is high 1/4 of pipe, and tampon sealing beyond the Great Wall is tied up, and using 1 hour of high-temperature sterilization, high-temperature sterilization condition is pressure
120 DEG C of 0.114MPa, temperature can become cordyceps sinensis mother culture media after cooling.
Index of correlation test:
It takes a commercially available cordyceps sinensis mother culture media as reference examples, tests the phase of each embodiment and comparative example and reference examples
Index is closed, the application method and condition of each culture medium are all the same, as a result summarize and are shown in Table 1.
Inoculation is with culture: separating strain from natural cordyceps, purifies, carries out molecular biology identification, determine that it is
Real aweto strain bacterial strain, is activating 1d for the parent species bacterial strain of cordyceps sinensis before inoculation, then strain in insulating box
Transfer room is moved into, in superclean bench by sterile working requirement, by it in the case where initial ph value is 6.0, to test tube slant center
Inoculation, inoculation block size are 5mm × 25mm.It moves into insulating box and cultivates after inoculation, condition of culture is 10-18 DEG C, no light.It connects
Every 2d record bacterium colony formation and hypha growth condition after kind.
Measurement mycelium length: mycelium length is the index of mycelia growth.By mycelium inoculation into slant medium, survey daily
1 colony diameter is measured, until bacterium colony full packages.
Count full packages number of days: full packages number of days is that strain is inoculated on slant medium, is covered with required for inclined-plane to mycelia
Time.
Observation mycelium growth vigor: mycelium growth vigor is the important indicator for evaluating hypha growth condition.With the whiteness of mycelia, life
Long robustness, dense degree of mycelia, bacterium platform observe and record the growing way of the mycelia of every kind of formula with a thickness of index.Observation knot
Fruit is indicated with very strong, strong, stronger, general, weak 5 grades.
Table 1
。
Claims (5)
1. a kind of cordyceps sinensis mother culture media, it is characterized in that it includes following raw materials according by weight: mulberry silkworm chrysalis powder 0.5-1.5
Part, 1-3 parts of carrot, 1-3 parts of potato, 0.5-1.5 parts of apple, 0.5-1.5 parts of peptone, 1-3 parts of glucose, potassium dihydrogen phosphate
0.05-0.15 parts, 0.05-0.15 parts of magnesium sulfate, 0.5-1.5 parts of agar, 0.5-1.5 parts of sorghum, 90-110 parts of water.
2. a kind of cordyceps sinensis mother culture media as described in claim 1, it is characterized in that it includes following original by weight
Material: 1 part of mulberry silkworm chrysalis powder, 2 parts of carrot, 2 parts of potato, 1 part of apple, 1 part of peptone, 2 parts of glucose, 0.1 part of potassium dihydrogen phosphate,
0.1 part of magnesium sulfate, 1 part of agar, 1 part of sorghum, 100 parts of water.
3. the preparation method of cordyceps sinensis mother culture media as described in claim 1, it is characterized in that including the following steps:
(1) being cut to 1cm or so fritter after carrot and potato and apple peel；
(2) above-mentioned three kinds of blocks and mulberry silkworm chrysalis powder and sorghum are added to the water and boil and is kept for 1 hour, obtain supernatant；
(3) teat glass is packed into after supernatant and peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, agar being sufficiently mixed
It is interior, using 0.5-1.5 hour of high-temperature sterilization, cordyceps sinensis mother culture media can be become after cooling.
4. the preparation method of cordyceps sinensis mother culture media as claimed in claim 3, it is characterized in that the high temperature of the step (3)
Sterilising conditions are: 120 DEG C of pressure 0.114MPa, temperature.
5. the preparation method of cordyceps sinensis mother culture media as claimed in claim 3, it is characterized in that the high temperature of the step (3)
Sterilization time is 1 hour.
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| CN110583360A (en) * | 2019-10-11 | 2019-12-20 | 云南农业大学 | Artificial culture method of ant cordyceps sinensis |
| CN112961778A (en) * | 2021-03-25 | 2021-06-15 | 云南乌蒙山国家级自然保护区管护局 | Universal wild edible fungus separation culture medium and preparation method thereof |
| CN116004393A (en) * | 2021-12-27 | 2023-04-25 | 江苏科技大学 | Phellinus linteus strain suitable for culturing mycelium with silkworm pupa, and culturing method and application thereof |
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| CN116004393A (en) * | 2021-12-27 | 2023-04-25 | 江苏科技大学 | Phellinus linteus strain suitable for culturing mycelium with silkworm pupa, and culturing method and application thereof |
| CN116004393B (en) * | 2021-12-27 | 2023-09-22 | 江苏科技大学 | A mulberry Phellinus mulberry strain suitable for culturing mycelium from silkworm pupae and its cultivation method and application |
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Application publication date: 20190118 |