CN109212015A - A kind of material surface oxidation-reduction quality detection method - Google Patents
A kind of material surface oxidation-reduction quality detection method Download PDFInfo
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- CN109212015A CN109212015A CN201811088958.5A CN201811088958A CN109212015A CN 109212015 A CN109212015 A CN 109212015A CN 201811088958 A CN201811088958 A CN 201811088958A CN 109212015 A CN109212015 A CN 109212015A
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- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 239000000463 material Substances 0.000 title claims abstract description 23
- 230000033116 oxidation-reduction process Effects 0.000 title description 4
- 239000000126 substance Substances 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 8
- 230000001590 oxidative effect Effects 0.000 claims description 6
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 4
- 230000001603 reducing effect Effects 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 18
- 239000012085 test solution Substances 0.000 abstract description 5
- 238000005259 measurement Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 11
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 5
- 239000011777 magnesium Substances 0.000 description 5
- 238000006722 reduction reaction Methods 0.000 description 5
- 229910052719 titanium Inorganic materials 0.000 description 5
- 239000010936 titanium Substances 0.000 description 5
- 229910052749 magnesium Inorganic materials 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 150000003536 tetrazoles Chemical class 0.000 description 3
- KJUGUADJHNHALS-UHFFFAOYSA-N 1H-tetrazole Chemical compound C=1N=NNN=1 KJUGUADJHNHALS-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 108091006149 Electron carriers Proteins 0.000 description 1
- 229910001069 Ti alloy Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000987 azo dye Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000007750 plasma spraying Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 238000010301 surface-oxidation reaction Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Electrochemistry (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of material surface oxidisability reproducibility detection methods, which is characterized in that specific step is as follows: (1) OD value of the preparation detection solution at 450nm is controlled 1.5；(2) the detection solution prepared is dripped in the material surface to be detected, then will test solution collection again；(3) the OD value of the detection solution that measurement is collected at 450 nm；(4) judge that material surface is with oxidisability or reproducibility according to gained OD value, OD value, which is greater than 1.5, has oxidisability, and OD value has reproducibility, OD value is constant, and substance is in stable state less than 1.5.This detection method is easy, can easily know the property of material surface.
Description
Technical field
The present invention relates to substance surface property detection technique fields, specially a kind of material surface oxidation-reduction quality detection side
Method.
Background technique
Plasma is by ion and electronics with positive and negative charge, it is also possible to which there are also the atom of some neutrality and molecule institutes
The aggregate of composition.Macroscopically generally in electroneutral.Plasma can be solid-state, liquid and gaseous state.Ionized gas is exactly
A kind of gaseous plasma.Basic process in plasma is the phase between various charged particles under the action of electric and magnetic fields
Interaction causes a variety of effects.Using it can be made to obtain a variety of applications the characteristics of plasma, electrician's development has been constituted
One frontier.
The application of plasma depends primarily on its property, after he is added to material surface, has material surface
Oxidizing or reproducibility, so that the function to realize it, still, specific material surface are with oxidisability or reduction
Property, exact it can not know there is no a specific detection method, so needing a kind of method now is able to detect substance
Surface is with oxidisability or reproducibility.
Summary of the invention
Present invention place against the above deficiency, provides a kind of material surface oxidation-reduction quality detection method, this detection is just
Method is easy, can easily know the property of material surface.
The technical solution adopted by the present invention to solve the technical problems is: a kind of material surface oxidisability reproducibility detection side
Method, which is characterized in that specific step is as follows:
(1) preparation detection solution controls OD value of the solution at 450 nm 1.5；
(2) the detection solution prepared is dripped in the material surface to be detected, then will test solution collection again；
(3) OD value of the detection solution that measurement is collected at 450 nm,
(4) judging that material surface is with oxidisability or reproducibility according to gained OD value, OD value, which is greater than 1.5, has oxidisability,
OD value has reproducibility, OD value is constant, and substance is in chemical stabilization state less than 1.5.
Further, the preparation method of detection solution that OD value is 1.5 at 450 nm is, by water-soluble tetrazole with
Then in physiological saline magnesium powder is added in the solution in 1:10 dilution proportion.
Further, 240 μ g magnesium powders are added in the water-soluble tetrazole solution that every 1 ml is diluted.
The principle of the present invention is water-soluble tetrazole in the cytoactive detection in cell Proliferation and cytotoxicity analysis
(WST) measurement gives sensitive colorimetric estimation.Water-soluble tetrazolium is reduced by the activity of dehydrogenase in the cell, shape
At a kind of first azo dyes of yellow color, culture medium is dissolved in.In the presence of electron carrier, WST can generate one kind
Reduction reaction occurs for water-soluble formic acid.In addition, WST also has the invertibity of chemical reaction, reductant-oxidant can be prepared.
Magnesium is a kind of strong reducing agent, and the oxidation of own can be reduced to the substance of surrounding.In the oxidation process of magnesium,
Release Mg2+ ion and two electronics.
Mg ⇌Mg2++2e-
WST can pass through several electron reductions to the WST of a color change.
WST has the invertibity of chemical reaction, wherein it is normal WST that the WST restored is oxidable.Due to normal WST
It cannot continue to aoxidize (no color change), and the OD value of the WST of reduction is up to 3 or more numerical value.Therefore, in order to determine etc. from
Whether daughter has oxidisability or reducibility, we control OD value of the WST at 1.5 using magnesium powder
WST0+ne- ⇌WST1.5+ne- ⇌ WST3
If plasma (P) has oxidability, WST can be made to restore, OD value increases.WST1.5+P ⇌ WST3
Alternatively, WST can be made to aoxidize if plasma (P) has reducibility, OD value is reduced
WST1.5+P ⇌ WST0
The oxidisability of liquid and the method for reproducibility and utensil how are only detected in existing technology, there is no to solid matter
The blank of the prior art, the method letter have been filled up in the detection method of surface oxidation and reproducibility, the appearance of the method well
Single easy, rapidly, the effect of let us plasma is understood more intuitively for detection.
Detailed description of the invention
It is titanium alloy surface OD value variation diagram before and after the processing shown in Fig. 1.
Specific embodiment
The present invention will be described in detail in the following with reference to the drawings and specific embodiments:
It as shown is a specific embodiment of the invention,
A kind of material surface oxidisability reproducibility detection method, which is characterized in that specific step is as follows:
(1) OD value of the preparation detection solution 450 nm at is controlled 1.5, by water-soluble tetrazole with 1:10 dilution proportion in
240 μ g magnesium powders are added in the water-soluble tetrazole solution that every 1 ml is diluted in physiological saline.
(2) the detection solution prepared is dripped in the material surface to be detected, then will test solution collection again；
(3) OD value of the detection solution that measurement is collected at 450 nm, detection method is the same as conventional.
(4) judge that material surface is with oxidisability or reproducibility according to gained OD value, OD value, which is greater than 1.5, has oxidation
Property, OD value is less than 1.5, and with reproducibility, OD value is constant, and substance is in chemical stabilization state.
Application example
Control group is detection solution,
Processing (1): will test solution and drip in metallic titanium surface, the solution then collected,
It handles (2): metallic titanium surface is subjected to NTAPPJ(non-thermal atmospheric pressure plasma
jet;Non-thermal atmospheric pressure plasma spraying machine, a kind of mode of Surface Treatment with Plasma) processing, it then will test solution drop
On processed titanium surface, solution is collected.
Above three groups of solution is carried out to the detection of OD value, as a result surface at 450 nm, handles the OD value of (1) and control group
As a result, the OD value of (2) is handled significantly lower than both other, this may be not have because titanium itself has stability to WST
The influence of any physics and chemical property, the reduction of processing (2) OD value means that oxidation reaction occurs for WST, in other words, on titanium
Plasma there is reducing property, make solution oxide, the variation of OD value is as shown in Figure 1.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811088958.5A CN109212015B (en) | 2018-09-18 | 2018-09-18 | Method for detecting surface oxidation-reduction property of substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811088958.5A CN109212015B (en) | 2018-09-18 | 2018-09-18 | Method for detecting surface oxidation-reduction property of substance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109212015A true CN109212015A (en) | 2019-01-15 |
| CN109212015B CN109212015B (en) | 2021-02-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
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Citations (7)
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|---|---|---|---|---|
| WO2001004346A1 (en) * | 1999-07-12 | 2001-01-18 | Xoma Technology Ltd. | Method to identify antimicrobial agents |
| CN1466683A (en) * | 2000-09-28 | 2004-01-07 | 爱科来株式会社 | Measuring method using redox reaction |
| JP2004000045A (en) * | 2002-05-31 | 2004-01-08 | Kyowa Hakko Kogyo Co Ltd | Antibodies to prostate-specific membrane antigen |
| CN1865942A (en) * | 2006-05-25 | 2006-11-22 | 广西亚热带作物研究所 | Method for fast detecting and positioning plant superoxide dismutase |
| US20080153121A1 (en) * | 2006-02-28 | 2008-06-26 | University Of Washington | Chemical Sensor Enhanced by Direct Coupling of Redox Enzyme to Conductive Surface |
| CN102262110A (en) * | 2010-05-28 | 2011-11-30 | 丁元生 | Color-developing grain for indicating microbial growth and preparation method thereof |
| CN106644997A (en) * | 2017-03-13 | 2017-05-10 | 江南大学 | Detecting method for detecting fruit and vegetable extract oxidation resistant capability by using gold nanometer material optical absorption characteristic |
-
2018
- 2018-09-18 CN CN201811088958.5A patent/CN109212015B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001004346A1 (en) * | 1999-07-12 | 2001-01-18 | Xoma Technology Ltd. | Method to identify antimicrobial agents |
| CN1466683A (en) * | 2000-09-28 | 2004-01-07 | 爱科来株式会社 | Measuring method using redox reaction |
| JP2004000045A (en) * | 2002-05-31 | 2004-01-08 | Kyowa Hakko Kogyo Co Ltd | Antibodies to prostate-specific membrane antigen |
| US20080153121A1 (en) * | 2006-02-28 | 2008-06-26 | University Of Washington | Chemical Sensor Enhanced by Direct Coupling of Redox Enzyme to Conductive Surface |
| CN1865942A (en) * | 2006-05-25 | 2006-11-22 | 广西亚热带作物研究所 | Method for fast detecting and positioning plant superoxide dismutase |
| CN102262110A (en) * | 2010-05-28 | 2011-11-30 | 丁元生 | Color-developing grain for indicating microbial growth and preparation method thereof |
| CN106644997A (en) * | 2017-03-13 | 2017-05-10 | 江南大学 | Detecting method for detecting fruit and vegetable extract oxidation resistant capability by using gold nanometer material optical absorption characteristic |
Non-Patent Citations (3)
| Title |
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| SHINYA KATO ET AL.: "Defensive effects of fullerene-C60/liposome complex against UVA-induced intracellular reactive oxygen species generation and cell death in human skin keratinocytes HaCaT, associated with intracellular uptake and extracellular excretion of fullerene-C60", 《JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B: BIOLOGY》 * |
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