CN109182434A - A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity - Google Patents
A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity Download PDFInfo
- Publication number
- CN109182434A CN109182434A CN201811205676.9A CN201811205676A CN109182434A CN 109182434 A CN109182434 A CN 109182434A CN 201811205676 A CN201811205676 A CN 201811205676A CN 109182434 A CN109182434 A CN 109182434A
- Authority
- CN
- China
- Prior art keywords
- quinoa
- preparation
- polypeptide
- hydrolysate
- obesity activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Child & Adolescent Psychology (AREA)
- Diabetes (AREA)
- Botany (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The preparation method of the invention discloses a kind of quinoa polypeptide hydrolysate with anti-obesity activity, comprising the following steps: take quinoa seed, removal of impurities, clear, drying milling, sieving obtains quinoa powder；2) protein in quinoa seed powder, and desiccation protein sample are extracted using alkali extraction and acid precipitation or physical method；3) protein sample that step 2) obtains is dissolved in proportion in distilled water, is proportionally added into digestive ferment enzyme and is hydrolyzed, by the centrifugation of obtained hydrolysate, drying, obtain polypeptide product.The preparation method of quinoa polypeptide of the invention is simple, easy to operate and environment friendly and pollution-free.There is fat-reducing effect by the quinoa polypeptide that above-mentioned preparation method is prepared.
Description
Technical field
The present invention relates to field of health care food, in particular to a kind of quinoa polypeptide hydrolysate with anti-obesity activity
Preparation method.
Background technique
With the continuous improvement of people's living standards, the high-incidence situation of obesity has caused the vast concern of people.
In obesity crowd, the disease incidence of coronary heart disease, hypertension, diabetes B even cancer is more much higher than lath.But due to subtracting
The side effect of fertile medicine and rebound effect, people thirst for a kind of health food with fat-reducing effect.
Quinoa, a kind of wholefood as high protein extensive concern by more and more people.Quinoa albumen
The amino acid ratio of matter balances and there is various physiological activity such as norcholesterol and glucoside inhibiting activity to have been confirmed.
Simultaneously studies have shown that under suitable conditions, enzymatic hydrolysis can improve the activity and functional characteristic of protein.Thus the present invention is ground
Study carefully the method for enzymatic hydrolysis preparation quinoa polypeptide, while having found its potential anti-obesity activity.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of quinoa polypeptide hydrolysate with anti-obesity activity
Preparation method, and be experimentally confirmed, which, which has, inhibits mouse embryonic fibroblasts (3T3-L1) in high sugar
The activity of fat cell is divided into culture medium.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions:
A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity, comprising the following steps:
1) quinoa seed, removal of impurities, clear, drying milling are taken, sieving obtains quinoa powder；
2) use conventional chemical extraction methods alkali extraction and acid precipitation (its principle for according to protein etc. make protein precipitation
Get off) or physical method such as use cyclone to extract the protein in separation quinoa seed powder, and desiccation protein sample；
3) protein sample that step 2) obtains is dissolved in proportion in distilled water, is proportionally added into digestive ferment enzyme and carries out water
The centrifugation of obtained hydrolysate, drying are obtained polypeptide product by solution.
Preferably, in above-mentioned technical proposal, drying temperature is 50 DEG C -70 DEG C, crosses 80 meshes after drying in the step 1).
Preferably, in above-mentioned technical proposal, the heavy method of alkali carries acid includes: in the step 2)
21) quinoa powder of seeds and 1M sodium hydroxide solution are subjected to mixed dissolution according to the solid-to-liquid ratio of 1:10, and use 0.1M
Sodium hydroxide or hydrochloric acid solution adjust pH value to 11, and concussion is overnight；
22) mixed liquor obtained above is centrifuged, uses the salt acid for adjusting pH of 0.1M to 3.5 after obtaining supernatant；
23) after standing 1 hour at 4 DEG C, centrifugation, freezing precipitation, drying obtain protein sample.
Preferably, in above-mentioned technical proposal, the condition being centrifuged in the step 22) and step 23) is 5000rap,
30min。
Preferably, in above-mentioned technical proposal, the step 3) specifically: will the obtained quinoa albumen of freeze-drying and distilled water by
It is mixed according to solid-to-liquid ratio 1:10-1:50, and pH is adjusted to 2, digestive ferment, additive amount and the quinoa albumen of the digestive ferment is added
Mass ratio is 1:10-1:50, and 80 DEG C of enzyme deactivation 20min after 120min are digested at 37 DEG C, obtained hydrolysate are centrifuged, supernatant
Liquid is freeze-dried to get quinoa polypeptide hydrolysate.
Preferably, in above-mentioned technical proposal, the solid-to-liquid ratio of the quinoa albumen distilled water is 1:20, and the digestive ferment adds
Dosage and quinoa albumen quality ratio are 1:20.
Preferably, in above-mentioned technical proposal, the digestive ferment in the step 3) is selected from pepsin, trypsase or wood
Melon protease it is one or more.
A kind of quinoa polypeptide hydrolysate with anti-obesity activity, is prepared according to above-mentioned preparation method
's.
A kind of quinoa polypeptide hydrolysate with anti-obesity activity preparation have anti-obesity activity drug and
Application in food.
Above-mentioned technical proposal of the present invention, has the following beneficial effects:
The preparation method of quinoa polypeptide of the invention is simple, easy to operate and environment friendly and pollution-free.Pass through above-mentioned preparation method system
Standby obtained quinoa polypeptide, using mtt assay detection to the activity influence of 3T3-L1 cell.Cytotoxicity experiment shows that quinoa is more
Peptide is the result shows that concentration is lower than in the case of 1600 μ g/ml, to 3T3-L1 cytotoxic；Research finds its concentration in 0-1600
It is particularly evident on the influence of 3T3-L1 cell activity within the scope of μ g/ml, it can significantly inhibit it to be divided into the physiology of fat cell
Process, and the expression of the PPAR γ inhibited, are presented dose dependent, that is, show that quinoa polypeptide has fat-reducing effect.
Detailed description of the invention
Fig. 1 is the preparation method flow chart of the quinoa polypeptide hydrolysate with anti-obesity activity of the invention.
Fig. 2 is inhibition situation map of the different quinoa hydrolyzed peptide additive amounts to 3T3-L1 adipocytic cell growth.
Fig. 3 is that Fig. 2 inhibits the corresponding histogram of situation map.
Specific embodiment
Specific embodiments of the present invention are described in detail below, in order to further understand the present invention.
All experimental methods used are conventional method unless otherwise specified in following embodiment.In following embodiment
Material, reagent used etc. can be obtained through commercial channels unless otherwise specified.
Embodiment 1
Pilot production is carried out according to 10 kilograms of quinoa seed raw materials, the preparation method is as follows:
(1) quinoa purchased removed into mineral particle, cleaned with clear water, 50 DEG C dry and obtained with milled 60 mesh of mill
To quinoa powder；
(2) protein in quinoa powder is extracted using the method that alkali carries acid sinks, quinoa powder and 1M sodium hydroxide solution is pressed
Mixed dissolution is carried out according to the solid-to-liquid ratio of 1:10, and with 0.1M and sodium hydroxide or hydrochloric acid solution adjusting pH value to 11, and shaken
Overnight.
Obtained mixed liquor is centrifuged (5000rap, 30min), obtain after supernatant with the hydrochloric acid of 0.1M adjust PH to
3.5；4 DEG C of standings were centrifuged (5000rap, 30min) after 1 hour, and were protein sample by pellet frozen drying.
(3) protein sample that freeze-drying obtains is dissolved in distilled water by the solid-to-liquid ratio of 1:20 and PH is adjusted to 2 with hydrochloric acid, and added
Enter the twentieth pepsin of albumen quality, 120 are digested at 37 DEG C, adjusts PH to 7.5,80 DEG C of enzyme deactivation 20min, and will
Supernatant is dried using the method for freeze-drying to get quinoa proteolysis polypeptide is arrived for obtained hydrolysate centrifugation
Product.
Embodiment 2
Pilot production is carried out according to 10 kilograms of quinoa raw materials, the preparation method is as follows:
(1) quinoa purchased removed into mineral particle, cleaned with clear water, 50 DEG C dry and obtained with milled 80 mesh of mill
To quinoa powder；
(2) protein in quinoa powder is extracted using the method that alkali carries acid sinks, quinoa powder and 1M sodium hydroxide solution is pressed
Mixed dissolution is carried out according to the solid-to-liquid ratio of 1:8, and with 0.1M and sodium hydroxide or hydrochloric acid solution adjusting pH value to 10, and shaken
Night.
Obtained mixed liquor is centrifuged (5000rap, 30min), obtain after supernatant with the hydrochloric acid of 0.1M adjust PH to
3.5；4 DEG C of standings were centrifuged (5000rap, 30min) after 1 hour, and were protein sample by pellet frozen drying.
(3) by distilled water that the obtained protein sample of freeze-drying is dissolved in by the solid-to-liquid ratio of 1:15 and to adjust PH with hydrochloric acid be 8.5,
And the twentieth trypsase of albumen quality is added, 120min is digested at 37 DEG C, adjusts PH to 7.5,80 DEG C of enzyme deactivations
20min, and obtained hydrolysate is centrifuged, supernatant is dried using the method for freeze-drying to get quinoa egg is arrived
Plain boiled water solution polypeptide product.
Embodiment 3
Pilot production is carried out according to 10 kilograms of quinoa raw materials, the preparation method is as follows:
(1) quinoa purchased removed into mineral particle, cleaned with clear water, 50 DEG C dry and obtained with milled 80 mesh of mill
To quinoa powder；
(2) protein in quinoa powder is extracted using the method that alkali carries acid sinks, quinoa powder and 1M sodium hydroxide solution is pressed
Mixed dissolution is carried out according to the solid-to-liquid ratio of 1:8, and with 0.1M and sodium hydroxide or hydrochloric acid solution adjusting pH value to 10, and shaken
Night.
Obtained mixed liquor is centrifuged (5000rap, 30min), obtain after supernatant with the hydrochloric acid of 0.1M adjust PH to
3.5；4 DEG C of standings were centrifuged (5000rap, 30min) after 1 hour, and were protein sample by pellet frozen drying.
(3) by distilled water that the obtained protein sample of freeze-drying is dissolved in by the solid-to-liquid ratio of 1:15 and to adjust PH with hydrochloric acid be 8.5,
And the twentieth papain of albumen quality is added, 120min is digested at 50 DEG C, adjusts PH to 7.5,80 DEG C of enzyme deactivations
20min, and obtained hydrolysate is centrifuged, supernatant is dried using the method for freeze-drying to get quinoa egg is arrived
Plain boiled water solution polypeptide product.
Fig. 2 is inhibition situation of the different quinoa hydrolyzed peptide additive amounts to 3T3-L1 adipocytic cell growth, as seen from the figure, agent
Measure higher, presentation dose-dependant higher to the growth inhibition ratio of fat cell.
A kind of quinoa polypeptide hydrolysate with anti-obesity activity, is prepared according to above-mentioned preparation method
's.
A kind of quinoa polypeptide hydrolysate with anti-obesity activity preparation have anti-obesity activity drug and
Application in food.
Specifically, including slimming medicine and diet food, the slimming medicine may also include the auxiliary agent medically allowed, subtract
Fertile food further includes other auxiliary materials.
The preparation method of quinoa polypeptide of the invention is simple, easy to operate and environment friendly and pollution-free.Pass through above-mentioned preparation method system
Standby obtained quinoa polypeptide, using mtt assay detection to the activity influence of 3T3-L1 cell.Cytotoxicity experiment shows that quinoa is more
Peptide is the result shows that concentration is lower than in the case of 1600 μ g/ml, to 3T3-L1 cytotoxic；Research finds its concentration in 0-1600
It is particularly evident on the influence of 3T3-L1 cell activity within the scope of μ g/ml, it can significantly inhibit it to be divided into the physiology of fat cell
Process, and the expression of the PPAR γ inhibited, are presented dose dependent, that is, show that quinoa polypeptide has fat-reducing effect.
Although the present invention is disclosed as above with embodiment, so it is not intended to limit the present invention, any those skilled in the art
Member, without departing from the spirit and scope of the present invention, can make a variety of different selections and modification, therefore protection model of the invention
It encloses and is limited by claims and its equivalents.
Claims (9)
1. a kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity, which is characterized in that including following step
It is rapid:
1) quinoa seed, removal of impurities, clear, drying milling are taken, sieving obtains quinoa powder；
2) protein in quinoa seed powder, and desiccation protein sample are extracted using alkali extraction and acid precipitation or physical method；
3) protein sample that step 2) obtains is dissolved in proportion in distilled water, is proportionally added into digestive ferment enzyme and is hydrolyzed,
By the centrifugation of obtained hydrolysate, drying, polypeptide product is obtained.
2. the preparation method of the quinoa polypeptide hydrolysate according to claim 1 with anti-obesity activity, feature
It is, drying temperature is 50-70 DEG C, crosses 80 meshes after drying in the step 1).
3. the preparation method of the quinoa polypeptide hydrolysate according to claim 1 with anti-obesity activity, feature
It is, the heavy method of alkali carries acid includes: in the step 2)
21) quinoa powder of seeds and 1M sodium hydroxide solution are subjected to mixed dissolution according to the solid-to-liquid ratio of 1:10, and with 0.1M hydrogen-oxygen
Change sodium or hydrochloric acid solution adjusts pH value to 11, concussion is overnight；
22) mixed liquor obtained above is centrifuged, uses the salt acid for adjusting pH of 0.1M to 3.5 after obtaining supernatant；
23) after standing 1 hour at 4 DEG C, centrifugation, freezing precipitation, drying obtain protein sample.
4. the preparation method of the quinoa polypeptide hydrolysate according to claim 3 with anti-obesity activity, feature
It is, the condition being centrifuged in the step 22) and step 23) is 5000rap, 30min.
5. the preparation method of the quinoa polypeptide hydrolysate according to claim 1 with anti-obesity activity, feature
It is, the step 3) specifically: mix quinoa albumen and distilled water that freeze-drying obtains according to solid-to-liquid ratio 1:10-1:50
Close, and pH be adjusted to 2, be added digestive ferment, the additive amount of the digestive ferment with；The mass ratio of quinoa albumen is 1:10-1:50,37
80 DEG C of enzyme deactivation 20min after enzymatic hydrolysis 120min, obtained hydrolysate are centrifuged, supernatant is freeze-dried to get quinoa egg at DEG C
White polypeptide hydrolysate.
6. the preparation method of the quinoa polypeptide hydrolysate according to claim 5 with anti-obesity activity, feature
It is, the solid-to-liquid ratio of the quinoa albumen distilled water is 1:20, and the additive amount and quinoa albumen quality ratio of the digestive ferment are 1:
20。
7. the preparation method of the quinoa polypeptide hydrolysate according to claim 1 with anti-obesity activity, feature
It is, the digestive ferment in the step 3) is one or more selected from pepsin, trypsase or papain.
It is preparation method described in -6 according to claim 1 8. a kind of quinoa polypeptide hydrolysate with anti-obesity activity
It is prepared.
9. the quinoa polypeptide hydrolysate according to claim 8 with anti-obesity activity in preparation there is weight-reducing to live
Application in the drug and food of property.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811205676.9A CN109182434A (en) | 2018-10-17 | 2018-10-17 | A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811205676.9A CN109182434A (en) | 2018-10-17 | 2018-10-17 | A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109182434A true CN109182434A (en) | 2019-01-11 |
Family
ID=64945414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811205676.9A Pending CN109182434A (en) | 2018-10-17 | 2018-10-17 | A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109182434A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116178492A (en) * | 2022-12-28 | 2023-05-30 | 海南健泰生物科技有限公司 | Quinoa pancreatic lipase inhibitory peptide and its application in the preparation of food for weight loss |
| CN116369535A (en) * | 2022-12-28 | 2023-07-04 | 海南健泰生物科技有限公司 | Quinoa weight-losing food and preparation method thereof |
| CN116178492B (en) * | 2022-12-28 | 2025-04-08 | 海南普利制药股份有限公司 | Quinoa pancreatic lipase inhibitory peptide and its application in preparing health products for weight loss |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106819356A (en) * | 2016-12-23 | 2017-06-13 | 钱斌 | A kind of plateau quinoa peptide and preparation method thereof and medicine-food two-purpose product |
| CN107805652A (en) * | 2017-09-26 | 2018-03-16 | 中国农业科学院作物科学研究所 | A kind of preparation method and application of the Semen phaseoli radiati polypeptide with active anticancer |
| CN108606246A (en) * | 2018-05-08 | 2018-10-02 | 内蒙古农业大学 | A kind of full cereal meal replacement powder of corn quinoa and preparation method thereof |
-
2018
- 2018-10-17 CN CN201811205676.9A patent/CN109182434A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106819356A (en) * | 2016-12-23 | 2017-06-13 | 钱斌 | A kind of plateau quinoa peptide and preparation method thereof and medicine-food two-purpose product |
| CN107805652A (en) * | 2017-09-26 | 2018-03-16 | 中国农业科学院作物科学研究所 | A kind of preparation method and application of the Semen phaseoli radiati polypeptide with active anticancer |
| CN108606246A (en) * | 2018-05-08 | 2018-10-02 | 内蒙古农业大学 | A kind of full cereal meal replacement powder of corn quinoa and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| GUIXING REN ET AL: ""Detection of lunasin in quinoa (Chenopodium quinoa Willd.) and the in vitro evaluation of its antioxidant and anti-inflammatory activities"", 《JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE》 * |
| 姜锡瑞等: "《生物发酵产业技术》", 31 May 2016, 中国轻工业出版社 * |
| 李波等: "《豆渣的营养功能特性与开发利用》", 31 August 2018, 科学技术文献出版社 * |
| 田旭静等: ""响应面法优化酶解藜麦糠蛋白制备抗氧化肽工艺"", 《食品科学》 * |
| 石振兴: ""国内外藜麦品质分析及其减肥活性研究"", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116178492A (en) * | 2022-12-28 | 2023-05-30 | 海南健泰生物科技有限公司 | Quinoa pancreatic lipase inhibitory peptide and its application in the preparation of food for weight loss |
| CN116369535A (en) * | 2022-12-28 | 2023-07-04 | 海南健泰生物科技有限公司 | Quinoa weight-losing food and preparation method thereof |
| CN116369535B (en) * | 2022-12-28 | 2024-10-11 | 海南普利制药股份有限公司 | Quinoa weight-losing functional food and preparation method thereof |
| CN116178492B (en) * | 2022-12-28 | 2025-04-08 | 海南普利制药股份有限公司 | Quinoa pancreatic lipase inhibitory peptide and its application in preparing health products for weight loss |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tao et al. | Yeast extract: characteristics, production, applications and future perspectives | |
| CN104152521B (en) | Peony pollen polypeptide and preparation method and application | |
| US20120309710A1 (en) | Compound sea cucumber preparation and manufacturing method thereof | |
| US20160317586A1 (en) | Method for the preparation of skipjack tuna extract having uric acid-lowering effect and the use thereof | |
| KR101467903B1 (en) | The preparing method of immune improving agents | |
| CN105341314A (en) | Buckwheat bran active protein extraction and separation technology | |
| CN103445159A (en) | Enzymatic preparation method of all-component ginseng juice | |
| CN108477612A (en) | A kind of dendrobium nobile nutritive powder and preparation method including dendrobium candidum purified extract | |
| CN110257459A (en) | Bird's nest small-molecular peptides and its instant Gly-His-Lys | |
| CN104491835A (en) | Assistant hypoglycemic health product containing Prunus padus L. polysaccharide | |
| CN110240626A (en) | A kind of quinoa polysaccharide polypeptide production method | |
| CN113249421B (en) | A kind of rubyrin polypeptide and its preparation and application | |
| WO2022032803A1 (en) | Extraction method for edible bird's nest, and extract and product thereof | |
| CN105663444A (en) | Compound immunity-enhancing and aging-resisting agent and preparation method thereof | |
| CN107090478B (en) | A kind of method for extracting water-soluble dietary fiber from shiitake stalk | |
| US20180008678A1 (en) | Preparation Method of the Albumin Peptide Combination and the Action of Inhibiting the Proliferation of Cancer Cells Thereof | |
| CN111657501A (en) | Oyster peptide cordyceps sinensis mycelium composition with function of enhancing immunity and application thereof | |
| CN109182434A (en) | A kind of preparation method of the quinoa polypeptide hydrolysate with anti-obesity activity | |
| CN106946997A (en) | The enhanced longan pulp polysaccharide of functional activity and its green modification method for preparing combined based on endogenous protein | |
| CN117925759B (en) | Wood frog polypeptide preparation and application thereof in food and cosmetics | |
| KR20210013482A (en) | Method for Extracting Active Ingredient of Herb Medicine and Composition for Enhancing Activity of Macrophage Prepared therby | |
| JP5794678B2 (en) | Glucagon-like peptide-1 secretion promoter | |
| CN102987408B (en) | Method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons | |
| KR101898711B1 (en) | Production method of amino acids powder using hydrolysis of Tenebrio molitor and the amino acids powder by this method | |
| CN117243380A (en) | Blood sugar reducing compound composition, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190111 |