A kind of high-efficiency plant extraction restorative procedure of uranium contaminated soil
Technical field
The invention belongs to contaminated soil remediation technical fields, and in particular to a kind of high-efficiency plant extraction of uranium contaminated soil is repaired
Compound method.
Background technique
Uranium (U) is both heavy metal and a kind of nucleic, has strong toxicity.China is mining big country, is successively built
A large amount of uranium mining area and uranium tailings pond are found.The mean concentration of China's topsoil (0-20cm) uranium is 3.03mg.kg-1,
And the U concentration of uranium tailings pond slag and contaminated soil is up to 26.11-122.1mg.kg-1.The mine generated is smelted in the exploitation of uranium ore
Slag, barren rock are polluted to caused by surrounding soil, farmland and water body, and the time is long and harmfulness is big, to around mine environment and people
The healthy and safe of the people masses constitutes serious threat.Therefore, it is one for the reparation of uranium contaminated soil urgently to be resolved to ask
Topic.
Nineteen eighty-three, American scientist Chaney are put forward for the first time phytoremediation technology.Compared to traditional restorative procedure, plant
Recovery technique has the advantages that the uniqueness such as safety, green, economy, is divided into four kinds according to its feature and purposes: plant extract (plant
Pollutant is removed from soil), phytovolatilization (plant by volatile pollutant removal), rhizosphere filtering (root system of plant from
Remove pollutant in the liquid of flowing), plant fix that (soil is changed into the lower form of toxicity by plant, but not from soil
Removal).Heavy metal pollution reparation for soil is generally fixed using plant extract and plant.
Phytoremediation circle generally assert that ideal landfill plant is super enriching plant at present.And it is heavy metal super-enriched
Four essential characteristics of plant are: (1) critical content feature: super enriching plant aerial part dry weight content is greater than centainly critical
Value;(2) transfer characteristic: the content of aerial part should be higher than that under ground portion;(3) patience characteristic: super enriching plant is to toxic contaminants
Object tolerance is high, particularly, the unobvious decline of Aboveground Biomass of Young when being grown on polluted soil;(4) concentration coefficient is special
Sign: concentration coefficient > 1 or even > 10.Numerous scientific workers are directed to the screening of uranium enriching plant, have carried out a large amount of research.
Although not yet finding the super enriching plant of uranium at present, it has been found that more than 20 plants have preferable enrichment energy to uranium element
Power, such as Indian mustard (Brassica juncea), sunflower (Helianthus annuus), dyers' grapes (Phytocacca
Americana L.), it is hot pickled mustard tuber (B.juncea var.tumida Tsen et Lee), argy wormwood (Artemisia argyi), yellow
Vanilla sweet-scented osmanthus (Melilotus officinalis), mountain jute (Trema orientalis), ambatch (Aeschynomene
Indica), Caulis Miscanthis floriduli (Miscanthus floridulus), cogongrass (Imperata cylindrica), macleaya cordata
(Macleaya cordata), kyllinga brevifolia (Kyllinga brevifolia), Herba chloranthi japonici Lettuce (Lactuca
Dolichophylla Kitam.), bracketplant (Chlorophytum comosum), dayflower (Commelina communis),
Iron amaranth (Acalypha australis L.), garden sorrel (Rumex acetosa Linn), witloof (Cichorium intybus
L.), fountain plant (Amaranth), water spinach (I aquatica Forsk), kidney bean (Phaseolus vulgaris
Var.humilis Alef), sorghum (Sorghum bicolor (L.) Moench), alternanthera philoxeroides (Alternanthera
Philoxeroides (Mart.) Griseb.), beggar-ticks (Bidens pilosa), Siberian cocklebur (Xanthium sibiricum),
Okra (Abelmoschus esculentus L.), cyperus iria (Cyperus iria L.), wandering jew (Zebrina
Pendula), yacon (Saussure a involucrata) etc..
Scholars also proposed it is various the above uranium enriching plant is used for the specific method of uranium contaminated soil remediation, but remain
In some problems:
(1) if rehabilitation plant belongs to the edible plants such as vegetables or fruit (such as Indian mustard, sunflower, hot pickled mustard tuber, the four seasons
Fragrant Lettuce, water spinach, witloof, kidney bean, sorghum, okra, yacon), then exist and enters food chain, eaten by mistake and serious by people
Influence the material risk of human health.
(2) dyers' grapes, argy wormwood, Melilot, ambatch, Caulis Miscanthis floriduli, cogongrass, macleaya cordata, kyllinga brevifolia, bracketplant, duck
Plantar grass, iron amaranth, garden sorrel, fountain plant, alternanthera philoxeroides, beggar-ticks, Siberian cocklebur, cyperus iria, wandering jew, hot pickled mustard tuber, Herba chloranthi japonici naked oats
The herbaceous plant biomass such as dish, water spinach, witloof is small, low so as to cause remediation efficiency.
(3) dyers' grapes, argy wormwood, Melilot, mountain jute, ambatch, Caulis Miscanthis floriduli, cogongrass, macleaya cordata, kyllinga brevifolia,
The plant protection measure of the wild plants such as dayflower, iron amaranth, garden sorrel, fountain plant, alternanthera philoxeroides, beggar-ticks, Siberian cocklebur, cyperus iria is not
It is perfect, lack the mature technology of large-scale plantation.
(4) the tolerance index of the not clear quantitative study uranium enriching plant of certain methods, such as hot pickled mustard tuber (B.juncea
Var.tumida Tsen et Lee), argy wormwood (Artemisia argyi), Melilot (Melilotus
Officinalis), mountain jute (Trema orientalis), ambatch (Aeschynomene indica), Caulis Miscanthis floriduli
(Miscanthus floridulus), cogongrass (Imperata cylindrica);Or research has shown that tolerance is poor, such as
Okra, fountain plant.The tolerance of uranium enriching plant is indefinite or tolerance is poor, will directly affect repairing effect.
(5) uranium contaminated soil is usually associated with other heavy metal pollutions, and certain uranium enriching plants lack to various heavy
Repair ability.
(6) most uranium enriching plants are root enriching plants, many scholars do not study its root exudates and
Rhizosphere surrounding soil environment is after rhizosphere is enriched with, and whether the activity of uranium reduces or whether toxicity weakens, and is just used for plant
It is fixed to repair.If the activity of uranium does not reduce or toxicity does not weaken after plant is fixed, if butt rot, a toxic huge sum of money
Category will return to soil, and the purpose for repairing pollution is not achieved.
(7) if using instruction plant, such as dyers' grapes carry out pollution amelioration, and large-scale plantation is likely to cause biology
Invasion problem.
Ramie (Boehmeria nivea), the perennial undershrub of Urticaceae Section of Genus Boehmeria or shrub, are the fibres for originating in China
Industrial crops are tieed up, some places in the Neolithic Age middle and lower reach of Yangtze River just have plantation, are known as " China's grass " in the world.World's ramie
Numb yield accounts for 90% of Gross World Product or so with Largest In China, and Brazil, Philippine occupy two, three, Indonesia, Korea,
There are a small amount of plantation in Vietnam and other countries in Southeast Asia, and Mediterranean Region also has artificial culture and successfully reports.
Authorization Notice No. discloses one kind for the Chinese patent of CN102919040B and plants on heavy metal intermediate pollution soil
Plant the method for ramie, cadmium, lead, arsenic, chromium, nickel, copper and the zinc that can be passivated in soil;Its object is to improve heavy-metal contaminated soil
Economic benefit, and for passivation after heavy metal can keep being passivated for a long time, plant ramie after soil heavy metal pollution
Whether situation, which makes moderate progress, is not directed to.
The Chinese patent of Publication No. CN108015104A discloses a kind of ramie of remediating heavy metal cadmium pollution soil
Numb implantation methods, but this method is only 0.47 and 0.52, respectively less than 1 for the concentration coefficient of cadmium respectively, institute is attainable
Repairing effect is extremely limited.
In order to improve ramie for the repairing effect of heavy metal, those skilled in the art have carried out some good tries.
The Chinese patent of Publication No. CN102165889A discloses a kind of for improving to Pb and As contaminated soil remediation
The ramie planting method of ability, this method can be improved by making between carrying out ramie and ciliate desert-grass to Pb and As contaminated soil
Repair ability.
The Chinese patent of Publication No. CN107952793A discloses a kind of is combined using Phanerochaete chrysosporium and ramie
The method in repairing and treating heavy metal pollution farmland, this method by by Phanerochaete chrysosporium and ramie combination, improve for
The repair ability of cadmium, arsenic and lead-contaminated soil.
But the research how repaired to uranium contaminated soil using plant extract repair mode is also less, and obtains
Achievement it is unsatisfactory.Therefore, the research of this respect is that this field is needed.
Summary of the invention
In view of the deficiencies of the prior art and demand, the purpose of the present invention is to provide a kind of high-efficiency plants of uranium contaminated soil
Restorative procedure is extracted, described method includes following steps:
(1) to being carried out site preparation and applying base manure by uranium contaminated soil;
(2) the ramie seed that sowing kind is Hunan ramie 7;
(3) after emergence grows young leaves, application growth fertilizer;
(4) ramie after growth is gathered in;
Wherein, it is described by uranium content in uranium contaminated soil be 25-175 μ g/g;
The base manure obtains by the following method:
1) by weight, prepare the following raw material:
8 parts of nano-sized carbon, 32 parts of urea, 6 parts of phosphoric acid diamines, 10 parts of potassium chloride, 6 parts of plant ash, 25 parts of dung polluted water, orange peel 12
Part, 3 parts of borax, 12 parts of ginkgo leaf, 1 part of azotobacter chroococcum, 2 parts of saccharomycete, 0.5 part of aspergillus niger;
2) nano-sized carbon, urea, phosphoric acid diamines, potassium chloride, plant ash, dung polluted water and borax are mixed, is stirred evenly, is inoculated with
Water is added in the ratio of solid and liquid weight ratio 1:100 in saccharomycete and azotobacter chroococcum, in the fermenter at 35-40 DEG C, anaerobic hair
Ferment 12-15 days, take tunning;
3) ginkgo leaf and orange peel are crushed, water are added in the ratio of solid and liquid weight ratio 1:8, stands 1 day at 20-25 DEG C,
Adjustment water content is 50-60%, and inoculated aspergillus niger carries out pile fermentation, when temperature reaches 60 DEG C, turning 1 time, and hereafter turning
3-4 times to maintain the temperature at 65-75 DEG C until decomposed, then spreading sunning to water content out is 20-25%;
4) step 2) and step 3) gains are mixed to get the base manure;
By weight, the formula of the growth fertilizer are as follows:
10 parts of paclobutrazol, 200 parts of urea, 40 parts of potassium sulfate, 150 parts of monoammonium phosphate.
In step (1), the method for the site preparation are as follows: after weeding, deep plough 0.3-0.4 meters, guarantee loosing soil, keep furrow face
Width is 2-4 meters.
In step (2), the method taken of sowing is that hand sowing method or dusting cover sieve broadcast method.The seedling root system of seed seedling-raising
Powerful, especially radish root is flourishing, is conducive to the uranium pollution for making full use of the under ground portion rhizome of ramie to extract soil, and inhale
It is strong to receive rich water ability, guarantees the vigorous growth of ramie, so the present invention is planted by the way of sowing.When mean daily temperature is 12
DEG C or more can sow
In step (2), when carrying out the sowing, application rate is 75 kilograms of seeds of every sq-km soil.
The amount of application of the base manure is 5 × 106Kg/ sq-km.
The fertilization mode of the growth fertilizer is that primary every fertilising in 5-7 days after seedling grows young leaves, each amount of application is
100g/ plants.
Before step (3) when emergence, thinning is carried out, density is 0.3m × 0.45m, every 2 young plant of cave.
In step (4), when correct fiber crops are gathered in, aerial part is gathered in when black bar reaches 2/3;Two fiber crops are gathered in
When, aerial part is gathered in when black bar reaches 2/3;After three fiber crops are mature, part above and below the ground is gathered in respectively.
After carrying out the harvesting, the recycling and processing of uranium are carried out.One of desirable method are as follows: on the ground by the ramie of harvesting
Part is concentrated respectively under ground portion dries burning, and aerial part ash content lower for uranium content can be filled;And for
The higher under ground portion ash content of uranium content, can further progress uranium recycling.
The study found that san ramie is often pioneer or the dominant plant in minery or smeltery's Tailings Dam, have
Shao Shui, few fertilizer are adapted to, the fine quality of Heavy Metal Contamination Stress is endured.The arsenic disulfide shimen Hunan realgar tail of Fia
Near sand dam, arsenic content is up to 284.9 μ g/g, only grows ciliate desert-grass, ramie;The arsenic content of ramie leaf reaches 536 μ g/g DW.
The persimmon bamboo plantation tungsten tin bismuth molybdenum composite ore mining area dressing plant the Cai Shan vegetal cover degree of Hunan Province's Chenzhou City only 5%, only Four Plants
Growth, ramie is one of them.Hunan Lengshuijiang Near Antimony Mine Area, 9 sampled point Sb contents are more than national soil background 40-
11503 times, but ramie belongs to dominant plant in this 9 sampled points.Ramie has enrichment to make on arsenic, cadmium, lead, antimony, copper, zinc etc.
With.Golconda Pb-Zn-Ag ore ramie sample has reached cadmium, the plant of lead super enrichment in critical content feature and two indexs of transfer characteristic
The standard of object.Shimen Hunan, Lengshuijiang, the mining area of Liuyang 3 ramie overground part Cd content it is bigger than the Cd content of general plant
2-10 times, As content is 9.9-147.5 times big, and Sb content is 1.2-338.4 times big;Cd concentration coefficient and transfer ratio peak are
2.07 with 3;As concentration coefficient and transfer ratio peak are 1.04 and 12.42, and Sb concentration coefficient and transfer ratio peak are
1.91 with 9.04.
The present inventor has carried out the sampling of 15 kinds of dominant plants in certain uranium tailings pond and analysis is tested, wherein ramie
Uranium accumulation ability and transfer ability it is most strong, sample root, stem, leaf uranium content be respectively 21.552,22.439 and
44.264 μ g/g, hence it is evident that higher than the 7.980 μ g/g of uranium content of its rhizosphere soil, concentration coefficient and transfer ratio are respectively 3.028
With 1.642, there is good uranium to be enriched with potential quality.
Inventor then uses ramie 7, artificial culture ramee variety Hunan to carry out the enrichment of ramie uranium and the potting of patience characteristic
Experiment.With 25,75,125,175,275,375,485 μ g/g, 7 various concentration uranium using saturation moisture capacity method preparation experiment soil
Earth, 4 repetitions of each concentration.Hunan ramie 7 uranium concentration coefficients and enrichments are calculated separately by stem, leaf, aerial part, under ground portion
Amount.The results showed that when soil uranium content is 25-175 μ g/g, Hunan ramie 7 uranium entirety concentration coefficients and under ground portion
Concentration coefficient increases with soil uranium content and is increased, respectively 1.200-1.834 and 1.460-2.341 (Fig. 1).Hunan ramie 7
Biomass and its under ground portion (table 1) is concentrated mainly on to the accumulation of uranium.By the maximum photochemistry amount of different uranium concentration for the treatment of
Suboutput, Aboveground Biomass of Young, plant height, root long average value and CK group average value carried out one-way analysis of variance (α=
0.05), and Tolerance index is calculated.To maximum Photochemical quantum yield, the aerial part of homogeneity test of variance conspicuousness > 0.05
Biomass, plant height carry out one-way analysis of variance using two methods of LSD and Duncan respectively.And homogeneity test of variance conspicuousness
< 0.05 root long is carried out respectively using tetra- kinds of Tamhane, Dunnett T3, Games-Howell (A), Dunnett C methods
The Multiple range test of post-hoc tests, comparison result show different disposal concentration group between without notable difference (table 2).It can be seen that Hunan ramie 7
Culture substrate number to uranium concentration between 25-485 μ g/g has quite high tolerance.
Since ramie is concentrated mainly on underground rhizome part (numb pocket) to the enrichment of uranium and association heavy metal, so by ramie
For the planting link of plant extract rehabilitating soil uranium pollution, carried out around the development of numb pocket is promoted as far as possible.Seed seedling-raising
Seedling root system it is powerful, especially radish root is flourishing, is conducive to the uranium for making full use of the under ground portion rhizome of ramie to extract soil
Pollution.
Above-mentioned discovery shows that ramie has natural abundance ability, patience strong uranium, and inventor passes through many experiments
After groping, after base manure and growth fertilizer of the invention are applied in discovery, the biomass of ramie and the richness for uranium can be significantly improved
Collection ability is more conducive to the reparation (table 3) of uranium contaminated soil, further improves and repairs uranium using it in a manner of plant extract
The efficiency of contaminated soil.In addition, ramie does not enter, food chain, growth is rapid, biomass is big, cultivation technique is mature, plant protection measure
It is perfect, underground rhizome is small in size, convenient for harvesting with centralized processing, be not present biotic intrusion problem.Therefore, using ramie as plant
Object, which extracts, repairs uranium contaminated soil with good application prospect.
Beneficial effects of the present invention:
Method of the invention can carry out efficient plant extract reparation to uranium contaminated soil, and ramie used does not enter food
Object chain, growth are rapidly, biomass is big, cultivation technique is mature, plant protection measure is perfect, patience is strong, underground rhizome is small in size, convenient for receipts
It cuts and focuses on, there is no biotic intrusion problem, the present invention is a kind of economic, reparation conveniently, high-efficient, profitable on ecology
The method of uranium contaminated soil.
Detailed description of the invention
Fig. 1 is the uranium content result figure of ramie No. 7 each sections in Hunan under comparative example different disposal concentration of the present invention.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
(1) site preparation and base manure are applied: first being extirpated weeds, killed subterranean pest-insect with a little pesticide.Deep ploughing is thin whole, general to require
0.3-0.4 meters of deep ploughing guarantees loosing soil;Wide general 2 meters of furrow face or so, according to topography, soil layer difference can width to 3-4 meters;Application
Base manure, the amount of application of base manure are 5 × 106Kg/ sq-km.
Above-mentioned base manure obtains by the following method:
1) by weight, prepare the following raw material:
8 parts of nano-sized carbon, 32 parts of urea, 6 parts of phosphoric acid diamines, 10 parts of potassium chloride, 6 parts of plant ash, 25 parts of dung polluted water, orange peel 12
Part, 3 parts of borax, 12 parts of ginkgo leaf, 1 part of azotobacter chroococcum, 2 parts of saccharomycete, 0.5 part of aspergillus niger;
2) nano-sized carbon, urea, phosphoric acid diamines, potassium chloride, plant ash, dung polluted water and borax are mixed, is stirred evenly, is inoculated with
Water is added in the ratio of solid and liquid weight ratio 1:100 in saccharomycete and azotobacter chroococcum, in the fermenter at 35-40 DEG C, anaerobic hair
Ferment 15 days, take tunning;
3) ginkgo leaf and orange peel are crushed, water are added in the ratio of solid and liquid weight ratio 1:8, stands 1 day at 20-25 DEG C,
Adjustment water content is 50-60%, and inoculated aspergillus niger carries out pile fermentation, when temperature reaches 60 DEG C, turning 1 time, and hereafter turning
3-4 times to maintain the temperature at 65-75 DEG C until decomposed, then spreading sunning to water content out is 20-25%;
Step 2) and step 3) gains are mixed to get the base manure;
(2) it sows.The seedling root system of seed seedling-raising is powerful, and especially radish root is flourishing, is conducive to make full use of ramie
Under ground portion rhizome extracts the uranium pollution of soil, and absorption rich water ability is strong, guarantees the vigorous growth of ramie, so of the invention
It is planted by the way of sowing.When mean daily temperature can be sowed at 12 DEG C or more.Repair 1km2Contaminated soil need 75kg
Seed.It broadcasts method using hand sowing method or dusting cover sieve and repeatedly sows back and forth.At 20 DEG C of mean daily temperature or so, a week
Emergence.According to emergence, in due course thinning.Planting density is advisable with 0.3m × 0.45m, the numb seedling in 2 plants of every cave.
(3) fertilising and management in growth period.Yangtze river basin ramie can receive for three seasons for 1 year, and general head fiber crops are at the end of May on June
In ten days, two numb late July to early Augusts, three fiber crops are in late October.After emergence grows young leaves, application growth fertilizer.Growth fertilizer is applied
Fertile mode are as follows: primary every fertilising in 5-7 days after seedling grows young leaves, each amount of application is 100g/ plants.By weight, raw
The formula to flesh up are as follows:
10 parts of paclobutrazol, 200 parts of urea, 40 parts of potassium sulfate, 150 parts of monoammonium phosphate.
In actual use, above-mentioned formula material is dissolved in 100000 parts of water and is applied fertilizer.
(4) head fiber crops, two fiber crops harvestings.After the numb straw of head grows into the regular period, the speed of growth obviously slows down, from top to bottom by
Gradual change is brown, suberification tissue occurs, and when black bar reaches 2/3, and lower part sesame slices fall off, and taper hand is pinched constantly, and the numb root and stem of certain plants, which has been sprouted, " urges
When root and stem of certain plants fiber crops " (two fiber crops), the harvesting of head fiber crops aerial part is carried out at this time.The new talent of sprouting continues to grow as two fiber crops, two dark bars
Its aerial part is gathered in after 2/3.
(5) three fiber crops harvestings: its aerial part is gathered in together under ground portion after after late October, three fiber crops are mature.
(6) focus on: the ramie aerial part of harvesting and under ground portion concentration dried into burning, ash content can further into
The processing such as row recycling uranium, landfill.
Comparative example
It is adjusted as follows on the basis of embodiment 1:
Base manure used is based on farmyard manure, supplemented by chemical fertilizer, slow-release fertilizer and quick-acting fertilizer cooperation, specific fertilizer and its every square
Km is used to be farmyard manure 15 × 106kg、3×106Kg dung polluted water, calcium superphosphate 37500kg.
Grow the application of fertilizer are as follows: when head fiber crops, two fiber crops, three Taro Asos are long vigorous, application growth fertilizer grows the fertilization mode of fertilizer
Are as follows: primary every fertilising in 5-7 days after seedling grows young leaves, each amount of application is 100g/ plants.By weight, fertilizer is grown
Formula are as follows:
10 parts of paclobutrazol, urea 200.
In actual use, above-mentioned formula material is dissolved in 100000 parts of water and is applied fertilizer.
For the technical effect for investigating embodiment 1 and comparative example, the present invention verified using potted plant experiment, with 25,75,
125,175,275,375,485 μ g/g, 7 various concentration uranium use saturation moisture capacity method preparation experiment soil, each concentration 4
It repeats.Uranium concentration coefficient and enriching quantity are calculated separately by stem, leaf, aerial part, under ground portion.In concrete operations, only
It does not need to carry out corresponding site preparation operation, other the step of operating with above-mentioned record are consistent.To embodiment 1 and comparative example
As a result it is detected, the result of comparative example is as shown in Fig. 1, Tables 1 and 2.It is found that Hunan ramie 7 for the uranium in uranium contaminated soil
With enrichment.Fertilizer used by comparative example is common fertilizer, we are significant to increase by changing applied fertilizer
Strong accumulation ability of the ramie for uranium.
From potted plant experiment it is found that the present invention can significantly increase accumulation ability, raising remediation efficiency of the ramie to uranium, have
Good plant extract repair function.In practical applications, it is only necessary to be used for big Tanaka according to the method for the present invention.
No. 7 Underground biomass of Hunan ramie and uranium accumulation and its proportion under 1 comparative example different disposal concentration of table
Hunan ramie 7 patience characteristics under 2 different disposal concentration of table
Note: ramie character Value Data is that (Aboveground Biomass of Young n=4, maximum are photochemical for mean+/-standard error in table 2
Learn quantum yield, plant height, root long n=8).
No. 7 Underground biomass of Hunan ramie and uranium accumulation and its proportion under 3 embodiment of table, 1 different disposal concentration