CN109170313A - A kind of anti-heat stress egg feedstuff compound additive and preparation method thereof - Google Patents

Abstract

The present invention relates to the composite feed additives for laying hen anti-heat stress; the compound being made of anti-heat stress composite extract and broker wall bee pollen; glycine betaine, taurine, the feed grade compound amino acid comprising methionine and lysine and suitable feed grade PURE WHEY composition；Wherein, the anti-heat stress composite extract extracts from the medicinal material group being made of honeysuckle, charred FRUCTUS CRATAEGI, dry chrysanthemum, scutellaria, wilsonii, Radix Glycyrrhizae.Additive of the present invention adjusts heat stress for laying hen various aspects and prepares, and can significantly improve immunity of layer chicken and anti-heat stress ability.

Description

A kind of anti-heat stress egg feedstuff compound additive and preparation method thereof

Technical field

The present invention relates to feed additive fields, in particular to a kind of to contain the days such as natural resisting stress extract and glycine betaine Laying hen compound resisting stress additive and preparation method thereof of right component.

Background technique

In modern poultry cultivation industry, due to the intensive of cultivation, breeding environment especially temperature, humidity and moisture are to influence An important factor for cultivation quality.Breeding environment can make animal generate stress reaction when unfavorable, and then inhibit the immune function of animal Can, it is chronically at the animal physiological dysfunction of stress situation, constitution decline, or even infectious disease.

In breeding layer chicken production, as laying hen is intensive, the development of large-scale production, especially in summer laying hen The probability of happening of heat stress is increasing.The laying hen production cycle is long, and summer high temperature easily causes heat stress, causes feed intake, lays eggs The decline of the indexs such as amount, feed conversion rate, oxidative damage aggravation, and it is unbalance with intestinal microflora, laying hen stops production when serious, Heavy economic losses is caused to breeding layer chicken.In addition, heat stress can cause poultry intestinal villi tissue degeneratiaon, gut cell membrane is penetrating Property increase, susceptible pathogenic microorganism, risk increases even dead.Studies have shown that heat stress refers to that animal body answers heat The physiological reaction of the nonspecific defense response of stimulus, its essence is refer to that environment temperature is non-more than caused by comfort zone ceiling temperature The summation of specific reaction.When serious, the physiological function that chicken can be changed reduces feed intake, daily gain, laying rate, makes egg type Become smaller, eggshell thinning becomes fragile, etc. even resulting in illness.In general, when giving up interior temperature rising to a certain extent, in feeding The excitement of pivot is partially inhibited, and feed intake is caused to decline.During heat stress, the energy that can be used in production of chicken sharply subtracts It is few, cause laying rate to decline.

The preferred ambient temperature of laying hen is 13-28 DEG C, reduces hen house temperature, adjustment daily ration knot in addition to taking at high temperature Outside structure, breeding heat-resistant strain measure, the prior art adds anti-heat stress drug in feed more and more to alleviate heat stress. But the influence of heat stress is that various, simple addition pharmaceutical preparations cannot overcome heat stress effect completely, this is because Resisting stress needs to be adjusted from many aspects, such as: central nervous system is adjusted, stable calm, reduction stress sensitive is played and makees With；Anti-oxidant, scavenging capacity oxygen radical, adjust by stress caused by Radical Metabolism disorder；Adjust body various substance generations Thank, adjust by stress caused by organism metabolism it is abnormal etc..In order to achieve the above object, need to take the addition of many-sided substance Such as vitamin, electrolyte, microelement, organic acid, antibiotic, although however the compound of multiple additives can be in certain journey Alleviate on degree stress, but above-mentioned multiple additives have not only broken up feed structure, and fecaluria excretion is caused to increase even digestion not Benign diarrhea, also increases aquaculture cost.Therefore, inexpensive Chinese herbal medicine and component can simply replace a variety of resisting stress substances Additive be breeding layer chicken there is an urgent need to.

In all resisting stress substances, glycine betaine, that is, trimethylglycine is excellent natural origin substance, into machine Body can be used as the donor of methyl during organism metabolism, and methyl is base necessary to neural, immune, kidney and cardiovascular system Group.Studies have shown that glycine betaine participates in the metabolism of vivo protein and fat, there are improvement diet palatability, reduction body fat to store Product maintains Premeabilisation of cells pressure and adjusts the biological functions such as cell electrolyte balance.But single glycine betaine is to irritability egg The effect of chicken is smaller, and studies at present about the complex additive of glycine betaine and Chinese medical extract less.

Currently, Chinese medicine is used as additive, directly addition digestibility after typically directly crushing or simply decocting extraction It is low, it is ineffective to also reduce diet palatability；And high dose is using being easy to bring the side effects such as food-intake decline even poisoning. Biological enzyme is gradually available for traditional Chinese medicine extraction at present.Such as patent CN201310604362.7 discloses a kind of mother chrysanthemum flower flavone The combined-enzyme method extraction process for closing object, belongs to technical field of extraction of Chinese traditional medicine.Its extraction step includes: by wild chrysanthemum drying, crushes, Cross the sieve of 300 mesh or so；It weighs dry powder and extractor is added, it is solvent that dehydrated alcohol, which is added, by liquid-to-solid ratio；It is added into tank compound Enzyme controls the conditions such as pH value, temperature in tank, digests, filtering；Filter vacuum is dried under reduced pressure, -20 DEG C be kept in dark place it is spare.Though Right biological enzyme extraction efficiency is higher, however, biological enzyme as protein, is easy deactivation in anhydrous ethanol solvent, Therefore its extraction effect is bad, is almost no different with alcohol extracting method effect.

In addition, Bee Pollen is also one of effective substance, recently as industry in natural resisting stress substance The appearance of Bee Pollen is commercialized, is also gradually applied as feed addictive.Specifically, Bee Pollen is a kind of natural battalion The product of supporting are the pollen of honeybee acquisition, in addition to the ingredient for having general pollen, also contain a small amount of nectar added in acquisition and divide Secretion.Bee Pollen has stronger physiological activity, improving immunocompetence；Amino acid, comprehensively dimension rich in component Raw element and vital movement indispensable such as phosphatide, organized enzyme, flavone compound, auxin, phytic acid, nucleic acid, swash at mineral element The substances such as element can replace most microelements and amino acids additive.Hormonal substance and polysaccharide conduct in pollen Bioactive substance is effective immunomodulator, its energy immune cell activated improves body's immunity, mostly important It is, and does not have toxic side effect to normal cell.

Processing extraction and application usually is carried out to Bee Pollen in the prior art.Such as CN1052417A is disclosed with overcritical two Carbonoxide carries out pollen to be deodorized fresh-keeping technical solution, provides one kind under conditions of lower than 40 DEG C, carries out to pollen Deodorization, it is fresh-keeping, and retain the bioactivity of its enzyme and the production technology of nutritive value.In supercritical fluid extraction equipment, answer With high-efficiency adsorbent, under conditions of temperature is lower than 40 DEG C and pressure is 6-22 megapascal (MPa), by CO 2 fluid to flower Powder carries out continuous extraction and absorption, can be obtained deodorization, fresh-keeping pollen.CN1307092A is disclosed to be extracted with CO 2 supercritical Fat compound in pollen pini is taken, the technical side that lipoid material is extracted in a kind of pollen pini from using wind as medium is provided Case.Pollen pini after drying, physical wall breaking or fermenting organism method broken wall and sterilization treatment is put into CO 2 supercritical In extraction equipment, enters extractor after the heated device heating of carbon dioxide after pressurization, come into full contact with pollen pini therein, Under conditions of temperature is 30-50 DEG C, pressure 8-40MPa, carbon dioxide flow rate are 0.5-15L/min, extract 0.5-2 hours, It respectively enters in firsts and seconds separator and separates after throttle expansion valve with the CO 2 fluid of fats extract. CN1733009 discloses a kind of method that fat compound is extracted from Bee Pollen, provides one kind and extracts from Bee Pollen The method of fat compound.After Bee Pollen is crushed merging be equipped with collet extractor, then by purity be 99% or more two Carbonoxide enters high-pressure pump after condenser is condensed into liquid, is sent into extraction after the heated device heating of the liquid carbon dioxide after pressurization Take device, come into full contact with powdery Bee Pollen, temperature is 30~65 DEG C, pressure is 20~40MPa, carbon dioxide flow rate be 20~ Under conditions of 50kg/h, continuous extraction 1~3 hour, the CO 2 fluid that carry extraction fat compound, which enters, to be equipped with The separator of collet, after the fat compound extracted separation, carbon dioxide returns to condenser.CN201110377240 is public A kind of method that nucleic acid and purifying are extracted from Bee Pollen is opened.Specific extracting method is: will be after screening, impurity elimination, drying Bee Pollen freezing carry out ultra-fine grinding, granularity reaches 20-60 mesh, and the 0.14mol/L sodium chloride of weight ratio 1: 8-10 is added Solution, homogenate, separates organelle by differential centrifugation, and at 20-30 kHz ultrasound 30-40 minutes, be allowed to crack To DNA- albumen, the 1mol/L sodium chloride solution of weight ratio 1:6-8 is then added, is homogenized, and in 20-30 kHz ultrasound 30- It 40 minutes, is allowed to cracking and obtains RNA- nucleoprotein, appropriate dodecyl sodium sulfate, which is added, separates protein and nucleic acid, is added dense Potassium acetate makes dodecyl sodium sulfate-protein complex precipitating, and extra dodecyl sodium sulfate is made to be converted into solubility Small sylvite and precipitate simultaneously, extract repeatedly, the pure solution of nucleic acid obtained using the method for ultracentrifugation or ion-exchange chromatography, adopt Nucleic acid pure material is obtained with vacuum freeze drying.

However, said extracted method process is complicated, process is cumbersome, and most of suitable laboratory is extracted with small-scale, meets Medicinal and edible purposes is not able to satisfy feed grade large-scale industrial production demand.Moreover, its separation often obtains a certain component, It causes and greatly wastes.

Therefore, although there are many drug or feed addictive of resisting stress, component and functions in the prior art Relatively simple, there is no the auxotype additives for integrating resisting stress and improving immunity of special suitable laying hen.

Summary of the invention

The technical problem to be solved by the present invention is to overcome prior art defect, a kind of raising laying hen resisting stress is provided especially It is the auxotype composite natral feed addictive and preparation method thereof of heat stress ability.

A primary object of the present invention is to overcome the unification of laying hen resisting stress preparation in the prior art, according to laying hen Heat stress stage various aspects regulatory demand provides compound anti-stress feed addictive；Another main purpose is to provide above-mentioned Feed has the preparation method for improving palatability.

Specifically, The technical solution adopted by the invention is as follows:

The first aspect of the invention provides a kind of anti-heat stress composite feed additive suitable for laying hen, comprising: press Parts by weight meter, 10-15 parts of compound be made of anti-heat stress composite extract and broker wall bee pollen, 3-5 parts of glycine betaine, ox sulphur Sour 0.5-1 parts and 1-3 parts of the feed grade compound amino acid comprising DL- METHIONINE and lysine and suitable feed grade cream Albumin powder.

Wherein, DL- METHIONINE and lysine amount ratio are 1:1-3 in the compound amino acid.

Wherein, the anti-heat stress composite extract is extracted from by honeysuckle, charred FRUCTUS CRATAEGI, dry chrysanthemum, scutellaria, thorn five Add, the medicinal material group of Radix Glycyrrhizae composition.

Preferably, medicinal material group each component content by weight are as follows: 5-6 parts of honeysuckle, 6-10 parts of charred FRUCTUS CRATAEGI, dry chrysanthemum 5-7 parts, 3-4 parts of scutellaria, 2-3 parts of wilsonii, 3-5 parts of Radix Glycyrrhizae of flower.

Wherein, dosage of the feed grade PURE WHEY in additive is the 50- of remaining mixture weight 100wt%.

Preferably, the feed addictive is the low water content particle pelletized by granulator, and preferred water content is low In 5%.

The second aspect of the invention provides the preparation method of above-mentioned anti-heat stress composite feed additive, comprising steps of

(S1) enzymatic hydrolysis-ultrasound alcohol extracting compound collecting method is taken to prepare anti-heat stress composite extract medicinal extract；

(S2) anti-heat stress extract-melissa flour complexes are prepared；

(S3) by anti-heat stress extract-melissa flour complexes and appropriate glycine betaine, taurine and compounded amino acid-mixed Conjunction obtains basic mixture；

(S4) PURE WHEY of additional proportion amount is uniformly mixed and prepares softwood and be granulated, dry, obtains the heat resistanceheat resistant and answers Swash feed addictive of layer chicken.

Specifically, specific step is as follows for the above method.

Step (S1): enzymatic hydrolysis-ultrasound alcohol extracting compound collecting method is taken to prepare anti-heat stress composite extract medicinal extract

(1) take it is dry and 5-6 parts of honeysuckle through dust removal process, 6-10 parts of charred FRUCTUS CRATAEGI, 5-7 parts of dry chrysanthemum, scutellaria 3-4 Part, 2-3 parts of wilsonii, 3-5 parts of Radix Glycyrrhizae, it crushed 80-100 mesh after above-mentioned material is mixed, and in mixed medicine material The middle complex cellulase pulvis that cellulase and hemicellulase composition is added；Wherein, medicinal powder poidometer, fiber are based on Plain enzyme and hemicellulose enzyme dosage are respectively 50-100U/g.

(2) above-mentioned medicinal powder is added to the distilled water of 10-15 times of weight, adjusts enzymolysis processing 3- at 5.0,50 DEG C of pH Then 5h adjusts pH 6.5-7.0 and heats and boils 15-20min enzyme deactivation, ultrasonic extraction after being cooled to 80-90 DEG C is super using 200W Sound extracts 2 times, each 25-30min, twice ultrasonic midfeather 30-60min；Filtrate is filtered and is concentrated after ultrasonic extraction, It obtains water and mentions filtrate.

(3) volume fraction for medicinal material filter residue being added to 5-6 times of quality (based on medicinal material dry weight) is molten for the ethyl alcohol of 70-80% In liquid, it is heated to 70-75 DEG C of continuation ultrasonic extraction 2 times, each 25-30min, twice ultrasonic midfeather 40-60min；Ultrasound Filtrate is filtered and be concentrated after extraction, obtains alcohol extracting filtrate；It is mentioned filtrate with above-mentioned water to merge, be concentrated under reduced pressure and adds water dilute Continue to be concentrated after releasing, repeats 1-2 times to no alcohol taste, obtain the extract medicinal extract for being equivalent to medicinal raw material weight 50-100wt%.

Step (S2): anti-heat stress extract-melissa flour complexes are prepared

Said extracted object medicinal extract and the broker wall bee pollen of 2-5 times of quality are mixed, and with 3-5wt% chitosan solution Adjustment solid concentration prepares particle softwood, pelletizes in high-speed stirred or granulator, dry.

Preferably, extract medicinal extract is mixed with the broker wall bee pollen of 3-5 times of quality.

Wherein, the Bee Pollen is selected from the feed grade Bee Pollen of any kind, the rape pollen being preferably commercialized, corn The Bee Pollens such as pollen, camellia pollen, above-mentioned pollen source is extensive, relative low price, can effectively replace amino acid, vitamin, The use of numerous feed addictives such as microelement and nucleic acid.

Preferably, the broker wall bee pollen sporoderm-broken rate is not less than 70% most desirably not less than 80%；Control pollen contains Within water 10%.Wherein, the broken wall means are arbitrary, including but not limited to cellulose enzyme process, supercritical ultrasonics technology, fermentation Method, ultramicro grinding method, extraction broken wall method, nanometer broken wall method and air-flow crushing broken wall method etc..

Preferably, the chitosan solution is low viscosity chitosan solution.

Preferably, the granule size of the particle is 10~100 mesh；More preferable 50~100 mesh.

Step (S3): compounding prepares basic mixture

By weight, by above-mentioned anti-heat stress extract -10-15 parts of Bee Pollen composite particles, feed grade glycine betaine 3-5 parts, 0.5-1 parts of taurine and 1-3 parts of feed grade compound amino acid comprising methionine and lysine are mixed to get basis Mixture.

Preferably, glycine betaine is 3 parts, 0.5 part of taurine.

Wherein, DL- METHIONINE and lysine quality amount ratio are 1:1-3 in the compound amino acid.

Preferably, methionine and lysine amount ratio are 1:1-2.

Wherein, the fodder beet alkali purity is not less than 98%, preferably not less than 99%；Its source can extract to be natural Or artificial synthesized, preferably artificial synthesized commodity glycine betaine, cheap and purity it is higher.

Step (S4): anti-heat stress feed addictive of layer chicken particle is prepared

The feed grade PURE WHEY for being equivalent to above-mentioned basic mixture weight 50-100wt% is added, uses after mixing evenly Chitosan containing 2-3wt%, 1-2wt% hydroxypropyl methylcellulose binder solution prepare softwood, pelletize in screen granulator, do It is dry, anti-heat stress egg feedstuff compound additive is made.

Preferably, the granulation granularity is 0.5-3mm, preferably 1-2mm.

Preferably, the feed addictive water content is lower than 5%, it is further preferred that being lower than 3%.

In the present invention, adding proportion of the additive in laying hen basal feed is 0.5-2wt%, preferably 1-1.5%.

Optionally, described adhesive is also selected from pectin, bentonite, concave convex rod, shallow lake other than hydroxypropyl methylcellulose One of powder, converted starch, sodium alginate are a variety of.

Other than anti-heat stress, additive of the present invention can also make an addition in the daily feed of other birds eat with Immunity of organisms and trophic level are improved, such as broiler chicken feed additive purposes.

Compared with prior art, the present invention at least has the following beneficial effects:

(1) present invention uses compound collecting method, takes enzyme to mention the compounding medicinal material with anti-stress effect and combines with alcohol extracting Means, improve efficiency about 30% compared to single extraction means, significantly improve recovery rate；Low raw-material cost, it is easy to operate, And obtain high recovery rate and purity.

(2) compared with prior art, the present invention takes the components such as the Chinese herbal medicine extract and broker wall bee pollen, glycine betaine multiple Match, and be added to the components such as taurine, methionine for laying hen, the body that gained preparation can preferably improve laying hen is resisted Power reduces energy consumption, improves anti-heat stress ability and the component that supplements the nutrients.

(3) Chinese herbal medicine extract that the present invention compounds has the function of heat-clearing, promotes to be immunized, and small with toxic side effect, Adjusting-nutritious multifunctional effect, passes through enzymolysis processing and compounds Bee Pollen and make wherein polysaccharide rich in, protein, ammonia Base acid, multivitamin and microelement, can effectively improve body resistivity, improve a poor appetite, and promote digestion, and enhancing adapts to Property, alleviate or eliminate the harm of heat stress.

(4) additive of the present invention is taken into account on the basis of resisting stress and enhancing immune function and adjusts energetic supersession and albumen Matter metabolism, the maintenance level of laying eggs and quality.Compared with chemical addition agent, meet food safety production requirement, after having preferably Wall effect.For example, wherein the Bee Pollen of broken wall is easier to be absorbed by organisms than pollen particles, various trace elements and amino are being provided On the basis of the nutriments such as acid, polypeptide, prevent tissue in laying hen body under the effect of extra free radical and oxidative stress, cell, DNA is damaged.And there is characteristic that is natural, having no toxic side effect, meet the needs of Modern Animal Husbandry environment-protecting feed formula.

(5) although wrapping up Chinese medical extract by Bee Pollen takes tool to further improve the palatability of feed Having the glycine betaine component of anti-stress effect improves bad smell, promotes the sensory effects of feed, promotes laying hen feed, is conducive to mention It is high its stress and immunocompetence, wherein taurine, chitosan etc. not only have the function of improving egg product, also have and recovery is promoted to answer Effect sharp and that raising is immune, each component are sought from adjusting central nervous system, improvement body Radical Metabolism disorder, adjusting body The different aspect for supporting the resisting stress such as metabolism exception acts synergistically, and achievees the effect that synergy.

(6) compound of the invention can not only improve the ability of laying hen resisting stress, and reduction brought stress injure, enhancing Immunity of organisms, moreover it is possible to improve efficiency of feed utilization, reduce feedstuff-egg ratio, be efficient, green, safe laying hen resisting stress and immune Reinforcing agent.

Specific embodiment

Below by specific preparation example and embodiment, the present invention is described in detail, but these exemplary embodiments Purposes and purpose be only used to enumerate the present invention, any type of any limit not is constituted to real protection scope of the invention It is fixed, it is more non-that protection scope of the present invention is confined to this.

The detailed description of preferred implementation method of the invention below and including embodiment can be more easily to understand it is of the invention Content.Unless otherwise defined, all technologies used herein and scientific term have and fields ordinary skill of the present invention The normally understood identical meaning of personnel.When there is a conflict, the definition in this specification shall prevail.

Terms used herein " by ... preparation " it is synonymous with "comprising".Term "comprising" used herein, " comprising ", " tool Have ", " containing " or its any other deformation, it is intended that cover non-exclusionism includes.For example, the composition comprising listed elements, Step, method, product or device are not necessarily limited to those elements, but may include not expressly listed other elements or such Composition, step, method, product or the intrinsic element of device.

Preparation example 1

(1) by weight, drying and 5 parts of honeysuckle through dust removal process are taken, it is 10 parts of charred FRUCTUS CRATAEGI, 7 parts of dry chrysanthemum, yellow It 4 parts of Cen, 3 parts of wilsonii, 3 parts of Radix Glycyrrhizae, crushes to sieve with 100 mesh sieve to obtain after above-mentioned material is mixed and extracts raw material 320g, and mixed The complex cellulase mixed powder being made of 30000U cellulase and 25000U hemicellulase is added in the medicine material of conjunction Agent.

(2) above-mentioned medicinal powder is added to the distilled water of 3.6L, is adjusted to enzymolysis processing at 5.0,50 DEG C of pH with dilute hydrochloric acid Then 5h adjusts pH to 7.0 and heats and boil 20min enzyme deactivation, using 200W ultrasonic extraction 2 times after being cooled to 80 DEG C, every time 25min, twice ultrasonic midfeather 30min；Filtrate is filtered and be concentrated after extraction, is obtained water and is mentioned filtrate.

(3) in extractor, filtered medicinal material filter residue is added in the ethanol solution that 1.8L volume fraction is 70%, It is heated to 70 DEG C of continuation ultrasonic extraction 2 times, each 30min, twice ultrasonic midfeather 60min；It is filtered after ultrasonic extraction And filtrate is concentrated, obtain alcohol extracting filtrate；It is mentioned filtrate with above-mentioned water to merge, revolving instrument is concentrated under reduced pressure into 300ml and adds water Continue to be concentrated into no alcohol taste after 600ml dilution, obtains about 200ml extract medicinal extract.

Preparation example 2

The broken wall rape flower Bee Pollen of whole extract medicinal extract of above-mentioned preparation and 660g are mixed, and use 3wt% Chitosan solution adjusts to obtain granulation softwood, pelletizes in High Speed Stirring Machine and drying to water content is lower than 5%, obtain about 708g extract-Bee Pollen composite particles.

Embodiment 1

(1) by weight, anti-heat stress extract -15 parts of Bee Pollen composite particles, feed are prepared by above-mentioned Grade 3 parts of glycine betaine, 0.5 part of taurine and comprising mass ratio be 1:2 methionine and 1 part of lysine compound amino acid mix To basic mixture 195g, gained basic mixture and 195g feed grade PURE WHEY are uniformly mixed；

(2) softwood is prepared with the binder solution of chitosan containing 3wt% (200cp viscosity), 1wt% hydroxypropyl methylcellulose, It pelletizes in screen granulator and drying to water content is lower than 5%, anti-heat stress egg feedstuff compound additive particle 408g is made, Granularity is about 1-1.1mm.

Embodiment 2

(1) by weight, anti-heat stress extract -10 parts of Bee Pollen composite particles, feed are prepared by above-mentioned Grade 4 parts of glycine betaine, 0.5 part of taurine and comprising mass ratio be 1:2 methionine and 3 parts of lysine compound amino acid mix To basic mixture 175g；

(2) above-mentioned basic mixture and 175g feed grade PURE WHEY are stirred evenly, with chitosan containing 4wt% The binder solution of (200cp viscosity), 1wt% hydroxypropyl methylcellulose prepares softwood, pelletizes in screen granulator and dry to containing Water is lower than 5%, and anti-heat stress egg feedstuff compound additive particle, granularity average out to 1.2mm is made.

Comparative example 1

By weight, the anti-heat stress extract-Bee Pollen composite particles 150g for taking above-mentioned preparation, with 195g feed Grade PURE WHEY is uniformly mixed；The preparation method is the same as that of Example 1 for remaining step, and additive D1 is made.

Comparative example 2

By weight, glycine betaine 40g and 195g feed grade PURE WHEY is taken to be uniformly mixed；The preparation of remaining step Additive D2 is made with embodiment 1 in method.

Effect example

1.1 test methods and grouping

The sea blue brown laying hen of health similar in 35 week old weight 600 is selected to be divided into 6 groups at random, every group of 4 repetitions, Mei Gechong Multiple 25 laying hens；Wherein normal group feeding basal feed, normal temperature environment (18-25 DEG C, humidity 60-65%)；Experimental group (1- 2 groups) and the contrast groups 1-2 addition that in Basic drawing prepared by the corresponding embodiment of addition 1wt% respectively under heat stress environment Agent (respectively corresponds embodiment 1-2, comparative example 1-2)；Blank control group only feeds basal feed under heat stress environment, does not add Any additive.

Test carries out in farm's totally enclosed type Laying House, three layers of staged cage.Experimental period each experimental group temperature wave Dynamic range is 27~34 DEG C (27~30 DEG C of evening temperature, 30~34 DEG C of day temperature；8 points of morning start to be gradually warmed up, 6 points of evening Cooling, the highest temperature on daytime are no more than 34 DEG C)；Average relative humidity is 75%~80%, constructs heat stress environment.

Experimental period 5 weeks, first week was the laundering period, and each daily additive therefor of test group (if any) is by test addition About the 1/3 of amount is gradually added to test dosage, and blank control group is without addition.All chickens are freely eaten, certainly during test By drinking water, natural lighting adds artificially feed to maintain daily 16h illumination；Artificial early, middle and late 3 feedings daily, routine disinfection and anti- Epidemic disease.

During test, the data such as feed intake, egg number, egg size, soft/broken egg number are recorded respectively by group daily, and measure chicken Egg Quality.All data are analyzed with the SPSS one-factor analysis of variance, and the significance level of difference is P < 0.05.

1.2 basal feed diet nutrients composition

Basal feed daily ration is to meet or with reference to laying hen battalion in National agricultural professional standard " fowl raising standard " (NY/T 33) The product that point recommendation is prepared is formed, the present embodiment uses and the corn-miscellaneous dregs of rice-premix type commodity diet of Meihua Co., examination Testing diet is powder type.Wherein basal diet, which is mainly organized, becomes (%): corn 58, dregs of beans 18, rapeseed dregs 12, peanut meal 10, Remaining is the additive premixes such as calcium monohydrogen phosphate, salt, multivitamin, nitrogen propylhomoserin, trace mineral；Feedstuff metabolisable energy 11.5MJ/KG, crude protein 16%.

The egg production (Kg/ is only) of 1.3 each groups, laying rate (%), average daily feed consumption rate (g), feedstuff-egg ratio (consume feed and lay eggs Ratio) and the data such as soft/rate of broken eggs (%) see the table below 1.

1 egg laying performance of table

As can be seen from the above table, laying rate of laying hen is on a declining curve under heat stress environment, and the test group containing additive Downward trend is relatively slow, it is shown that the positive effect for alleviating test chicken heat shock.Meanwhile test group average daily gain and normal Group is substantially suitable, is not significantly affected, and test group feed to egg ratio is significantly improved than control group, to test 2 groups of improvement width Degree is maximum, substantially close to normal group.The result shows that under the high temperature conditions, experimental group and control group especially blank control group ratio Egg production, laying rate can be relatively significantly improved, and significantly improves food consumption doses, reduce feedstuff-egg ratio, difference has conspicuousness (P < 0.05) illustrates that additive of the present invention can preferably improve Layer Production Performance under heat stress state.

The egg qualities indexs such as the average egg weight (g) of 1.4 test groups and control group, shell thickness (mm), Eggshell weight (g) Each group chicken illness number of elements see the table below during (last day randomly selects 6 pieces of measurements from each repetition weekly), and test.

2 egg quality of table

The result shows that experimental group significantly improves Eggshell weight and shell thickness than control group, and egg size is improved, while significant It improves laying hen health rate and reduces illness rate, reduction stress be dead.Illustrate that Abwehrkraft des Koepers can be improved in additive of the present invention, and shows Write the anti-heat stress ability for improving laying hen.

To sum up, additive of the present invention has enhancing or adjusts and exempt from by immunopotentiator-resisting stress extract multi-effect cooperative Epidemic disease function is taken into account and adjusts the metabolisms such as energetic supersession and protein, and laying hen caused by mitigation heat stress is in disorder significantly, subtracts The excessive loss of nutriment and energy under few heat stress state；Meanwhile additive of the present invention has nutriment concurrently and is immunized anti- Stress double action, strengthen immunity and anti-heat stress on the basis of supplementing nutrient, thus reach laying rate, egg size increase Add, feedstuff-egg ratio reduces, and feed conversion rate improves and other effects.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution The range of scheme.

Claims (10)

1. a kind of composite feed additive for laying hen anti-heat stress, which is characterized in that by weight, comprising: heat resistanceheat resistant is answered Swash composite extract and broker wall bee pollen composition 10-15 parts of compound, 3-5 parts of glycine betaine, 0.5-1 parts of taurine and comprising 1-3 parts of the feed grade compound amino acid and suitable feed grade PURE WHEY of DL- METHIONINE and lysine；Wherein, described Anti-heat stress composite extract extract from the medicinal material being made of honeysuckle, charred FRUCTUS CRATAEGI, dry chrysanthemum, scutellaria, wilsonii, Radix Glycyrrhizae Group.

2. feed addictive as described in claim 1, which is characterized in that DL- METHIONINE and bad ammonia in the compound amino acid Sour amount ratio is 1:1-3.

3. feed addictive as described in claim 1, which is characterized in that by weight, the medicinal material group each component content Are as follows: 5-6 parts of honeysuckle, 6-10 parts of charred FRUCTUS CRATAEGI, 5-7 parts of dry chrysanthemum, 3-4 parts of scutellaria, 2-3 parts of wilsonii, 3-5 parts of Radix Glycyrrhizae.

4. the preparation method of feed addictive as described in claim 1-3, which comprises the steps of:

(S1) enzymatic hydrolysis-ultrasound alcohol extracting compound collecting method is taken to prepare anti-heat stress composite extract medicinal extract；

(S2) anti-heat stress extract-melissa flour complexes are prepared；

(S3) anti-heat stress extract-melissa flour complexes are mixed with appropriate glycine betaine, taurine and compound amino acid To basic mixture；

(S4) PURE WHEY of additional proportion amount is uniformly mixed and prepares softwood and be granulated, dry, obtains the anti-heat stress egg Chicken feed addictive.

5. preparation method as claimed in claim 4, which is characterized in that (S1) specific step is as follows:

(1) take it is dry and 5-6 parts of honeysuckle through dust removal process, 6-10 parts of charred FRUCTUS CRATAEGI, 5-7 parts of dry chrysanthemum, 3-4 parts of scutellaria, It 2-3 parts of wilsonii, 3-5 parts of Radix Glycyrrhizae, crushed 80-100 mesh after above-mentioned material is mixed, and add in mixed medicine material Enter the complex cellulase of cellulase and hemicellulase composition；

(2) above-mentioned medicinal powder is added to the distilled water of 10-15 times of weight, adjusts enzymolysis processing 3-5h at 5.0,50 DEG C of pH, so PH 6.5-7.0 is adjusted afterwards and is heated boils 15-20min enzyme deactivation, ultrasonic extraction after being cooled to 80-90 DEG C, using 200W ultrasonic extraction 2 times, each 25-30min, ultrasonic midfeather 30-60min twice；Filtrate is filtered and be concentrated after ultrasonic extraction, obtains water Mention filtrate；

It (3) is to be heated to 70-75 DEG C in the ethanol solution of 70-80% by the volume fraction that medicinal material filter residue is added to 5-6 times of quality Continue ultrasonic extraction 2 times, each 25-30min, twice ultrasonic midfeather 40-60min；It is filtered after ultrasonic extraction and dense Contracting filtrate obtains alcohol extracting filtrate；It is mentioned filtrate with above-mentioned water to merge, be concentrated under reduced pressure and continues to be concentrated after being diluted with water, is repeated To no alcohol taste, extract medicinal extract is obtained 1-2 times.

6. preparation method as claimed in claim 4, which is characterized in that (S2) specific step is as follows:

Said extracted object medicinal extract and the broker wall bee pollen of 2-5 times of quality are mixed, and adjusted with 3-5wt% chitosan solution Solid concentration prepares particle softwood, pelletizes in high-speed stirred or granulator, dry.

7. preparation method as claimed in claim 6, which is characterized in that the Bee Pollen be selected from rape pollen, zasiokaurin, Camellia pollen, and broken wall of melissa pollen rate is not less than 70%.

8. preparation method as claimed in claim 4, which is characterized in that (S3)-(S4) specific step is as follows:

(1) by weight, by anti-heat stress extract -10-15 parts of Bee Pollen composite particles, 3-5 parts of feed grade glycine betaine, 0.5-1 parts of taurine and 1-3 parts of feed grade compound amino acid comprising methionine and lysine are mixed to get basic mixture；

(2) the feed grade PURE WHEY for being equivalent to above-mentioned basic mixture weight 50-100wt% is added, uses after mixing evenly Chitosan containing 2-3wt%, 1-2wt% hydroxypropyl methylcellulose binder solution prepare softwood, pelletize simultaneously dry, obtained heat resistanceheat resistant is answered Swash egg feedstuff compound additive.

9. the purposes that feed addictive described in claim 1-3 is used for laying hen basal feed additive.

10. purposes as claimed in claim 9, which is characterized in that adding proportion of the additive in laying hen basal feed For 0.5-2wt%.