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CN109168877A - A kind of method that suitable industrial seedling rearing improves Fraxinus velutina hardwood cutting rooting rate - Google Patents

A kind of method that suitable industrial seedling rearing improves Fraxinus velutina hardwood cutting rooting rate Download PDF

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Publication number
CN109168877A
CN109168877A CN201811089277.0A CN201811089277A CN109168877A CN 109168877 A CN109168877 A CN 109168877A CN 201811089277 A CN201811089277 A CN 201811089277A CN 109168877 A CN109168877 A CN 109168877A
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cuttings
cutting
cuttage
matrix
rooting rate
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燕丽萍
王因花
姚俊修
吴德军
刘翠兰
任飞
李庆华
王开芳
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Shandong Academy of Forestry
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Shandong Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

本发明公开了一种适宜工厂化育苗快速提高绒毛白蜡硬枝扦插生根率的方法,是选择1~2年的硬枝作为插穗,剪好的枝条用浓度为75%乙醇+800~1000mg/L NAA+400~500mg/L IBA速蘸,然后插入由珍珠岩:蛙石:椰糠按体积为4:2:1的比例混合制成基质的育苗容器中,扦插后在湿度90%以上,温度36‑44℃条件下生根。实验证实本发明方法扦插成活率高达98.3%,根系发达,带基质移栽,移栽成活率高,生根时间能缩短20多天,2个月出圃,省时省力,管理方便,适用于大规模工厂化育苗,能广泛满足良种化生产的需求,具有极大的应用与推广价值。

The invention discloses a method suitable for industrial seedling rearing to rapidly increase the rooting rate of cuttings of P. chinensis hardwoods. NAA+400~500mg/L IBA was quickly dipped, and then inserted into a seedling container made of perlite:frog stone:coconut bran mixed in a volume ratio of 4:2:1 to make a matrix. After cutting, the humidity was above 90%, and the temperature Rooted at 36-44°C. Experiments have confirmed that the cutting survival rate of the method of the invention is as high as 98.3%, the root system is developed, the transplanting with substrates, the transplanting survival rate is high, the rooting time can be shortened by more than 20 days, and the nursery is produced in 2 months, saving time and labor, convenient management, and suitable for large-scale Factory seedlings can widely meet the needs of improved seed production, and have great application and promotion value.

Description

A kind of method that suitable industrial seedling rearing improves Fraxinus velutina hardwood cutting rooting rate
Technical field
The invention belongs to technical field of plant propagation, and in particular to a kind of suitable industrial seedling rearing quickly improves Fraxinus velutina The method of hardwood cutting rooting rate.
Background technique
Chinese wax (Fraxinus) is Oleaceae (oleaceae) Fraxinus (Fraxinus) plant general designation, and the whole world is existing white Wax belongs to more than 70, and about 34 kinds of China, foreign countries introduce about 10 kinds, spreads each provinces and regions.Wherein Fraxinus velutina (Fraxinus It velutina) is the higher Precious Timber Species of economic value and saline-alkali soil forestation vanguard tree seed.Traditional Fraxinus velutina is asexual Breeding is mainly bred using grafting, and the period is long, breeds low efficiency, at high cost, constrains the popularization and application of Chinese wax excellent variety. The features such as cutting propagation is simple and easy, the breeding cycle is shorter, inexpensive efficient and holding elite stand good characteristic, is widely used in producing Practice.However, Chinese wax cuttage raise seedling technique is always the technical problem produced in nursery, the slotting research of dialogue candlestick at present is reported It is very few.Growth regulator is studied to gold leaf Chinese wax using the spray for giving birth to semi-lignified for the year as material in road refined (2018) etc. The influence of (Fraxinus chinensis ' Jinguan ') cuttage root-taking and enzymatic activity, the results showed that 200mg/LIBA+ The effect that 400mg/L IAA impregnates cuttings 30s is best, rooting percent 94.44%.Zhao Shuan etc. (2018) is with gold leaf Chinese wax (Fraxinus chinensis ' Jinyebaila ') stiff wood and shoot cutting seedling are test material, analyze different cutting mediums, The shadow of cuttage period, plant growth regulator type, No. 1 soaking concentration of ABT and soaking time to Survival rate of nursery stock and situation of taking root It rings.The result shows that: cutting medium is up to 75% with turfy soil+thin river sand+perlite (volume ratio 1:1:2) survival rate;Most preferably Cuttage period is March and June, 70% or more shoot cutting survival rate, the place of No. 300mg/LABT1 immersion cuttings base portion 30min It is best to manage effect, 70% or more survival rate.Liang Youwang (2010) etc. is used using current year life and 2 years raw branches as cuttings material Different disposal method carries out cuttage and seedling culture test to evergreen Chinese wax (Fraxinus griffithii), the results showed that, annual shoot Raw rooting efficiency was good than 2 years for cuttage root-taking effect, with concentration was 500mg/L ABT 1 in the middle part of evergreen Chinese wax 1 year raw branch Root-inducing powder handles 10s, and rooting rate is up to 60%.Ye Jingfeng (2011) etc. is with half wood of White Ash (Fraxinus Americana) Matter spray is cuttings, has studied the shadow of different management methods and different plant growth regulator treatment White Ash cuttage and seedling cultures It rings, the results showed that No. 1 root-inducing powder of 100mg/L ABT handles 6h, and rooting rate is up to 93%.He Li (2017) etc. is to section Chinese wax (Fraxinus hupehensis) the spring tip and autumn growth spray are cuttings, have studied carefully callus after various concentration IBA, NAA aqueous solution soaking The influence of rate, rooting rate, height of seedling, ground diameter, internode number.The result shows that: the rooting rate of spring tip cuttings is imitated in IBA125mg/L processing Fruit is best, rooting rate 77%.The rooting rate of autumn growth cuttings is best in NAA150mg/L treatment effect, and rooting rate is up to 80.0%. Yan Liping (2016, patent No. ZL201410532141.8) etc. is 80 with concentration using No. 5 stiff branches of 1~2 year Shandong wax as cuttings It after~120mg/L ascorbic acid (vitamin C) and 80~120mg/L soaking with indole butyric acid 12h, then with concentration is 1000ppm's Special efficacy rooting powder solution impregnates 2~4min, and rooting rate is up to 93.3% or more.
Above studies have shown that different Chinese wax inter-species, in plant growth regulator type, handles time, concentration and matrix It is larger that difference is influenced on rooting rate.The different Chinese wax kind cuttage root-taking difference are larger, this may be related with Chinese wax genotype.
At present about the research of Fraxinus velutina, only 2 reported in literature, Yao Junxiu (2015) etc. is white with 2 years raw villus Wax (Fraxinus velutiwa) seedling spray is cuttings, using three factors (hormone kind, hormone concentration and processing time) Three horizontal quadrature experimental designs study Chinese wax softwood cutting propagation technology under different cuttage environment.The result shows that: 200mg/LNAA Handle optimal when 2h, cuttage survival rate is up to 100%.Zhang Jianguo etc. is using 1 year raw healthy and strong branch to Fraxinus velutina garden wax 2 (Fraxinus velutiwa yuanla2) cuttage raise seedling technique is studied, the scion that will be sheared, by the sand in a winter Hiding, then with concentration be No. 2 processing 8h of 200mg/L root-growing agent, rooting rate is up to 87%.However, using traditional hardwood cutting method, After needing one winter of sand storage, second year 3-5 month cuttage, cuttings is easy mildew, and operating method is cumbersome, time-consuming and laborious, exists The long defect of growing-seedling period, seedling cost is high and cuttage survival rate difficulty is secure.To sum up, the quick skewer of Fraxinus velutina batch production stiff wood It is slotting breeding be still nursery production in technical problem, this field is still blank, Fraxinus velutina hardwood cutting breeding method and Improving cuttage survival rate is still current project urgently to be resolved.
Summary of the invention
In view of the deficiencies of the prior art, the problem to be solved in the present invention is to provide a kind of suitable industrial seedling rearings quickly to improve The method of Fraxinus velutina hardwood cutting rooting rate, this method cuttings need not move through the sand storage in a winter, quickly dipped, time saving province Power, method are easy.Fraxinus velutina hardwood cutting Rapid Rooting and breeding may be implemented using the method.
The method that suitable industrial seedling rearing of the present invention improves Fraxinus velutina hardwood cutting rooting rate, step is:
(1) greenhouse is arranged: plastic foil scaffolding, top are covered with shading net, and automated spray, water system is good, and draining is logical Freely;
(2) Fraxinus velutina hardwood cutting matrix design and preparation;
(3) cutting medium sabot: the cutting medium disinfected is dispensed into seedlings nursing plate, and cutting medium thickness is not less than 8cm is sprinkled profoundly water, spare;
(4) prepared by cuttings: spring being taken to select the stiff branch of 1-2 healthy and strong, that no disease and pests harm is not germinateed as cuttings;
(5) it is pre-processed before cuttings cuttage;
(6) cuttings cuttage method: pretreated cuttings is inserted into matrix, and cuttage depth is 6 ± 1cm, is then irrigated Water, and the matrix around cuttings and jack is compacted;
(7) postcondition control and management are inserted;
(8) after cuttage root-taking, Kei removes shading net, carries out full exposure hardening;
It is characterized by:
Step (2) the Fraxinus velutina hardwood cutting matrix design and the method for preparation are: the component of cutting medium is precious Zhu Yan, frog stone and coco bran, mixed proportion are perlites: frog stone: coco bran is 4:2:1 by volume;1000 are used after being mixed Times liquid carbendazim sterilization, cuttage before repeat sterilizing one time for 24 hours;
Pretreated method is before step (5) described cuttings cuttage: by the cuttings concentration trimmed be 75% ethyl alcohol+ 800~1000mg/LNAA+400~500mg/L IBA solution is quickly dipped, then directly carries out cuttage operation;
The method of step (7) the slotting postcondition control and management is: being protected to the cuttings after cuttage using auto spraying method It is wet, make relative air humidity control 90% or more, temperature control is at 36~44 DEG C and continues 45 ± 1d, to improve cutting survival Rate.
Above-mentioned suitable industrial seedling rearing improves in the method for Fraxinus velutina hardwood cutting rooting rate: step (5) described cuttings Pretreated method is preferably before cuttage: being 75% ethyl alcohol+800mg/LNAA+400mg/L by the cuttings concentration trimmed IBA solution is quickly dipped, then directly carries out cuttage operation.
Above-mentioned suitable industrial seedling rearing improves in the method for Fraxinus velutina hardwood cutting rooting rate: step (7) is described insert after The method of condition control and management is preferably: to the cuttings after cuttage using the moisturizing of auto spraying method, making relative air humidity control For system 90% or more, temperature control is at 42 DEG C and continues 45d.
The invention discloses a kind of methods that suitable industrial seedling rearing quickly improves Fraxinus velutina hardwood cutting rooting rate, should Method has the advantage that is with beneficial effect:
1, the method for the present invention is bred for stiff wood, and cuttings concentration is 75% ethyl alcohol+800mg/L NAA+400mg/L IBA It is quickly dipped, and high humidity high temperature method is used, it effectively prevents polluting, makes cuttage root-taking high survival rate up to 98.3% or more, significantly improve Cuttage survival rate.
2, Fraxinus velutina hardwood cutting and seedling raising method disclosed by the invention, without overwintering Sand culture, method is easy, saves Shi Shenggong is easily promoted and applied, and rooting efficiency is good, and seedling raise period is short, it can be achieved that extensive nursery production, it is white to solve villus The technical problem of wax hardwood cutting breeding.
Detailed description of the invention
Fig. 1: 1 month cuttage rooting.
Fig. 2: 3 months cuttage rootings.
Fig. 3: the upgrowth situation of 1 month root of cuttage.
Fig. 4: the upgrowth situation of 3 months roots of cuttage.
Specific embodiment
Embodiment 1
(1) greenhouse is arranged: plastic foil scaffolding, top are covered with shading net, and automated spray, water system is good, and draining is logical Freely.
(2) cutting medium design and preparation: cutting medium is by perlite: frog stone: coco bran is mixed in the ratio that volume is 4:2:1 Conjunction is made, and is carried out disinfection sterilizing before cuttage for 24 hours with 1 000 times of carbendazim.
(3) sabot: the matrix disinfected being dispensed into 4 × 4 × 8cm seedlings nursing plate, is sprinkled profoundly water, spare.
(4) prepared by cuttings: March 28, acquiring healthy and strong, no disease and pests harm the, age was that the stiff branch of 1-2 is cuttings, Branch average length is 8~12cm, acquires 200 cuttings.For branch upper end clip away from straight snips at upper 1~2cm of bud, lower clip is oblique It cuts;
(5) Pretreatment: the cuttings concentration trimmed is 75% ethyl alcohol+800mg/LNAA+400mg/L IBA speed It dips in, can be used to cuttage.
(6) cottage method: pretreated cuttings is inserted into matrix, and cuttage depth is 6cm, is then sprinkled profoundly water, and will Matrix compacting around cuttings and jack;
(7) it is managed after inserting: using the moisturizing of auto spraying method, making relative air humidity control 90% or more, temperature controls In 42 DEG C of lasting 45d, to improve cuttage survival rate.
(8) after cuttage 45d, Kei removes shading net, carries out full exposure hardening.
As a result it counts, rooting rate 98.3%.
Embodiment 2
In addition to cuttings processing concentration is that 75% ethyl alcohol+900mg/LNAA+450mg/L IBA is quickly dipped, remaining operation and reality It is identical to apply example 1, statistical result after 45d, rooting rate 98.1%.
Embodiment 3
In addition to managing relative air humidity control after inserting 90% or more, temperature is controlled at 40 DEG C, remaining operation and implementation Example 1 is identical, statistical result after 45d, and rooting rate is rooting rate 98.0%.
Embodiment 4
In addition to cuttings processing is quickly dipped with 1000mg/L IBA, 1000mg/L NAA and 1000mg/L ABT1 respectively, remaining behaviour Make same as Example 1, statistical result after 45d, rooting rate is respectively 53.6%, 64.7 and 73.8%.
Embodiment 5
Preparing in addition to cutting medium is respectively perlite or perlite: vermiculite: turf volume ratio is 2:2:1, remaining operation Same as Example 1, statistical result after 45d, rooting rate is respectively 79.5% and 81.8%.
Embodiment 6
In addition to 60% or more, temperature is controlled at 24-28 DEG C for management relative air humidity control after inserting, remaining operation with it is real It is identical to apply example 1, statistical result after 45d, rooting rate is rooting rate 11.3%.

Claims (3)

1.一种适宜工厂化育苗提高绒毛白蜡硬枝扦插生根率的方法,步骤是:1. a method that is suitable for industrialized seedling raising and improves the rooting rate of P. chinensis hardwood cuttings, the steps are: (1)温室设置:塑料膜搭棚,顶部覆有遮阴网,自动化喷雾,供水系统良好,排水通畅;(1) Greenhouse setting: a plastic film canopy, the top is covered with a shade net, automatic spraying, a good water supply system, and smooth drainage; (2)绒毛白蜡硬枝扦插基质设计及制备;(2) Design and preparation of cutting substrates of P. tomentosa hardwood; (3)扦插基质装盘:将消毒好的扦插基质分装到育苗盘中,扦插基质厚度不低于8cm,浇透水,备用;(3) cuttage substrate loading: the sterilized cuttage substrate is divided into seedling trays, and the thickness of the cutting substrate is not less than 8cm, and water is permeable, for subsequent use; (4)插穗制备:取春季选择健壮的、无病虫害未发芽的1-2年生硬枝条作为插穗;(4) preparation of cuttings: take the 1-2 year hard branches selected in spring and select robust, no diseases and insect pests and do not germinate as cuttings; (5)插穗扦插前预处理;(5) pretreatment before cuttings; (6)插穗扦插方法:将预处理后的插穗插入基质中,扦插深度为6±1cm,然后浇透水,并将插穗与插孔周围的基质压实;(6) cutting method for cuttings: insert the pretreated cuttings into the matrix, the cutting depth is 6 ± 1cm, then pour water, and compact the cuttings and the matrix around the jack; (7)插后条件控制及管理;(7) Post-insertion condition control and management; (8)扦插生根后,掲除遮阴网,进行全光照炼苗;(8) After the cutting takes root, remove the shade net, and carry out full-illumination and hardening; 其特征在于:It is characterized by: 步骤(2)所述绒毛白蜡硬枝扦插基质设计及制备的方法是:扦插基质的组分是珍珠岩、蛙石和椰糠,其混合比例是珍珠岩:蛙石:椰糠按体积为4:2:1;混合制成后采用1000倍液多菌灵消毒灭菌,扦插24h前再重复灭菌一遍;The method of step (2) described in fluffy ash hardwood cutting matrix design and preparation is: the component of cutting matrix is perlite, frog stone and coconut bran, and its mixing ratio is perlite: frog stone: coconut bran is 4 by volume: 2:1; after mixing, 1000 times liquid carbendazim was used for disinfection and sterilization, and the sterilization was repeated 24 hours before cutting; 步骤(5)所述插穗扦插前预处理的方法是:将修剪好的插穗用浓度为75%乙醇+800~1000mg/LNAA+400~500mg/L IBA溶液速蘸,然后直接进行扦插操作;The method for pretreatment before cutting of the cuttings in step (5) is as follows: the pruned cuttings are quickly dipped in a solution with a concentration of 75% ethanol+800~1000mg/LNAA+400~500mg/L IBA, and then the cutting operation is directly performed; 步骤(7)所述插后条件控制及管理的方法是:对扦插后的插穗采用自动喷雾法保湿,使空气相对湿度控制在90%以上,气温控制在36~44℃并持续45±1d,以提高扦插成活率。The method for controlling and managing the post-insertion conditions in step (7) is as follows: adopt automatic spraying to keep the cuttings moisturizing, so that the relative air humidity is controlled at more than 90%, and the air temperature is controlled at 36~44° C. for 45±1d, To improve the survival rate of cuttings. 2.根据权利要求1所述适宜工厂化育苗提高绒毛白蜡硬枝扦插生根率的方法,其特征在于:步骤(5)所述插穗扦插前预处理的方法是:将修剪好的插穗用浓度为75%乙醇+800mg/LNAA+400mg/L IBA溶液速蘸,然后直接进行扦插操作。2. according to the described method of claim 1, it is characterized in that: the method for pretreatment before the described cuttings and cuttings of step (5) is: the cuttings that are pruned are used with a concentration of 75% ethanol+800mg/LNAA+400mg/L IBA solution was quickly dipped, and then the cutting operation was carried out directly. 3.根据权利要求1所述适宜工厂化育苗提高绒毛白蜡硬枝扦插生根率的方法,其特征在于:步骤(7)所述插后条件控制及管理的方法是:对扦插后的插穗采用自动喷雾法保湿,使空气相对湿度控制在90%以上,气温控制在42℃并持续45d。3. according to claim 1, it is characterized in that: the method for condition control and management after the described insertion of step (7) is: to the cutting after the cutting, adopt automatic Moisturizing by spray method, the relative humidity of the air is controlled above 90%, and the temperature is controlled at 42°C for 45 days.
CN201811089277.0A 2018-09-18 2018-09-18 A kind of method that suitable industrial seedling rearing improves Fraxinus velutina hardwood cutting rooting rate Pending CN109168877A (en)

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CN103430749B (en) * 2013-08-30 2014-09-10 河南科技大学 Seedling growing method for promoting fast rooting of plant cutting slips under high-temperature and high-humidity conditions
CN104529656A (en) * 2015-01-14 2015-04-22 郑州农达生化制品有限公司 Nursery stock growth promoting and thickening agent
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RJ01 Rejection of invention patent application after publication

Application publication date: 20190111

RJ01 Rejection of invention patent application after publication