CN109142728A - The kit of pancreatic elastase 1 and its application in a kind of quantitative determination excrement - Google Patents
The kit of pancreatic elastase 1 and its application in a kind of quantitative determination excrement Download PDFInfo
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- CN109142728A CN109142728A CN201811024574.7A CN201811024574A CN109142728A CN 109142728 A CN109142728 A CN 109142728A CN 201811024574 A CN201811024574 A CN 201811024574A CN 109142728 A CN109142728 A CN 109142728A
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- Prior art keywords
- pancreatic elastase
- buffer
- kit
- reagent
- excrement
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- 102100035371 Chymotrypsin-like elastase family member 1 Human genes 0.000 title claims abstract description 51
- 101000737684 Homo sapiens Chymotrypsin-like elastase family member 1 Proteins 0.000 title claims abstract description 50
- 108010067372 Pancreatic elastase Proteins 0.000 claims abstract description 59
- 102000016387 Pancreatic elastase Human genes 0.000 claims abstract description 59
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 46
- 239000004816 latex Substances 0.000 claims abstract description 44
- 229920000126 latex Polymers 0.000 claims abstract description 44
- 239000000872 buffer Substances 0.000 claims abstract description 39
- 239000002245 particle Substances 0.000 claims abstract description 35
- 239000003381 stabilizer Substances 0.000 claims abstract description 31
- 239000003755 preservative agent Substances 0.000 claims abstract description 26
- 230000002335 preservative effect Effects 0.000 claims abstract description 24
- 239000000427 antigen Substances 0.000 claims abstract description 22
- 102000036639 antigens Human genes 0.000 claims abstract description 22
- 108091007433 antigens Proteins 0.000 claims abstract description 22
- 239000003792 electrolyte Substances 0.000 claims abstract description 21
- 239000004094 surface-active agent Substances 0.000 claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims description 30
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000004132 cross linking Methods 0.000 claims description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000007853 buffer solution Substances 0.000 claims description 10
- 239000006193 liquid solution Substances 0.000 claims description 10
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 9
- 229940098773 bovine serum albumin Drugs 0.000 claims description 9
- 238000010382 chemical cross-linking Methods 0.000 claims description 9
- 239000007987 MES buffer Substances 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- 230000003213 activating effect Effects 0.000 claims description 8
- 230000004204 exocrine pancreatic function Effects 0.000 claims description 8
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- 239000003431 cross linking reagent Substances 0.000 claims description 6
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 235000002639 sodium chloride Nutrition 0.000 claims description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000594 mannitol Substances 0.000 claims description 5
- 235000010355 mannitol Nutrition 0.000 claims description 5
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 4
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 239000005018 casein Substances 0.000 claims description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 4
- 235000021240 caseins Nutrition 0.000 claims description 4
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- 235000011147 magnesium chloride Nutrition 0.000 claims description 4
- -1 polyoxyethylene phenyl ether Polymers 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 3
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 claims description 3
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 claims description 3
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 3
- 239000007995 HEPES buffer Substances 0.000 claims description 3
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 3
- 239000008351 acetate buffer Substances 0.000 claims description 3
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 claims description 3
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 claims description 3
- 229940042795 hydrazides for tuberculosis treatment Drugs 0.000 claims description 3
- 150000002466 imines Chemical class 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 229920000151 polyglycol Polymers 0.000 claims description 3
- 239000010695 polyglycol Substances 0.000 claims description 3
- CCMKPCBRNXKTKV-UHFFFAOYSA-N 1-hydroxy-5-sulfanylidenepyrrolidin-2-one Chemical compound ON1C(=O)CCC1=S CCMKPCBRNXKTKV-UHFFFAOYSA-N 0.000 claims description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims description 2
- 239000007993 MOPS buffer Substances 0.000 claims description 2
- ZGABFIHCVASNKH-UHFFFAOYSA-N [K].N=C=O Chemical compound [K].N=C=O ZGABFIHCVASNKH-UHFFFAOYSA-N 0.000 claims description 2
- 150000002191 fatty alcohols Chemical class 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 239000002953 phosphate buffered saline Substances 0.000 claims description 2
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical group ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 claims 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 16
- 238000005259 measurement Methods 0.000 abstract description 8
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 238000004879 turbidimetry Methods 0.000 abstract description 8
- 230000002708 enhancing effect Effects 0.000 abstract description 7
- 238000003556 assay Methods 0.000 abstract description 4
- 238000002649 immunization Methods 0.000 abstract description 3
- 230000003053 immunization Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000002835 absorbance Methods 0.000 description 9
- 210000000496 pancreas Anatomy 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 239000012452 mother liquor Substances 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 102000016942 Elastin Human genes 0.000 description 3
- 108010014258 Elastin Proteins 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000003908 quality control method Methods 0.000 description 3
- 108010085895 Laminin Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- BXGTVNLGPMZLAZ-UHFFFAOYSA-N n'-ethylmethanediimine;hydrochloride Chemical group Cl.CCN=C=N BXGTVNLGPMZLAZ-UHFFFAOYSA-N 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000000668 Chronic Pancreatitis Diseases 0.000 description 1
- 101710138848 Chymotrypsin-like elastase family member 1 Proteins 0.000 description 1
- 101710099240 Elastase-1 Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 1
- 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 206010033649 Pancreatitis chronic Diseases 0.000 description 1
- 101710180319 Protease 1 Proteins 0.000 description 1
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 description 1
- ZGBSOTLWHZQNLH-UHFFFAOYSA-N [Mg].S(O)(O)(=O)=O Chemical compound [Mg].S(O)(O)(=O)=O ZGBSOTLWHZQNLH-UHFFFAOYSA-N 0.000 description 1
- 235000010358 acesulfame potassium Nutrition 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000012490 blank solution Substances 0.000 description 1
- 150000001642 boronic acid derivatives Chemical group 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 108010011793 cholesterol binding protein Proteins 0.000 description 1
- 102000049842 cholesterol binding protein Human genes 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 210000003499 exocrine gland Anatomy 0.000 description 1
- 210000003020 exocrine pancreas Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 108010057670 laminin 1 Proteins 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000009256 replacement therapy Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000003871 sulfonates Chemical class 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of kits of pancreatic elastase 1 in quantitative determination excrement, including 1 antigen calibration object solution of R1 reagent, R2 reagent and pancreatic elastase；R1 reagent includes electrolyte, stabilizer, surfactant, preservative and buffer；R2 reagent includes latex particle, electrolyte, stabilizer, surfactant, preservative, the buffer for being coated with anti-human 1 polyclonal antibody of pancreatic elastase；1 antigen calibration object solution of pancreatic elastase includes pancreatic elastase 1 and stabilizer.The kit is a kind of device with pancreatic elastase 1 in latex enhancing immune turbidimetry for Determination excrement; it is accurate, time saving and energy saving to stablize; the effect of the clinical detection assays of full-automatic quickly measurement sample can be achieved; compared with traditional immunization is than turbid reagent; detection sensitivity improves 10 times or more; polyclonal antibody is coupled to latex particle surface by the method being chemically crosslinked, and the effective protection active region of antibody and antigen binding improves detection sensitivity.
Description
Technical field
The invention belongs to medical test and vitro diagnostic techniques field, it is related to pancreatic elastase 1 in a kind of measurement excrement
Kit, relate in particular to a kind of utilize pancreatic elastase in latex enhancing immune turbidimetry quantitatively determining human excrement
1 kit and its application.
Background technique
Excrement pancreatic elastase 1 (Fecal elastase 1, FE-1) is a kind of pancreas inscribe by exocrine gland secretion
Protease is discharged into duodenum through duodenofiberscope, concentration can specifically prompt to occur when reducing chronic pancreatitis or
The infull situation of exocrine pancreatic function.Excrement pancreatic elastase 1 has stability in enteron aisle, by detecting it in excrement
Content can reflect the function of exocrine pancreas indirectly, it is this method safety, noninvasive and do not influenced by pancreatin replacement therapy.But
It is that the method that currently used immunochromatography detects excrement pancreatic elastase 1 has that accuracy and specificity are low, and grasps
Make complicated, detection inconvenience.
Latex enhancing immune turbidimetry (Latex-enhanced t μ rbidimetric imm μ noassay) is a kind of body
The white homogeneous transmission immunological turbidimetry detection method of liquid eggs, principle are the surface-crosslinked Anti-TNF-αs in nanoscale polymer latex microballoon
Body can flock together rapidly in a short time after the microballoon and antigen binding for being crosslinked with antibody, change the saturating of reaction solution
Optical property；The change of reaction solution light transmission (i.e. absorbance) and the concentration of tested antigen have stronger correlation, in certain model
It can reflect the concentration of tested antigen in enclosing.It has the advantages that 1, is time saving and energy saving: being resisted in homogeneous reaction system
Former, antibody response, directly measures the absorbance value of reaction solution using automatic clinical chemistry analyzer, a few minutes can obtain as a result,
Enzyme-linked immunization is save to be incubated for repeatedly and the tedious operation steps such as board-washing；2, it is accurate to stablize: the simplification of operating procedure is also correspondingly
The interference of the extraneous factors such as many manual operation factors and reagent, environment is avoided, stability and repeatability are all preferable, can be serious
Reflect the content of pancreatic elastase 1 in tested blood of human body or excrement on the spot；3, it is widely used: latex enhancing immune
The sensitivity of turbidimetry is enough to detect the lower limit value of pancreatic elastase 1, can fully meet clinical detection requirement, hence it is evident that be better than
Immunochromatographic method (colloidal gold).But in the prior art, it there is no at present and utilize latex enhancing immune turbidimetry for Determination human excrement and urine
The related kit and detection method of middle pancreatic elastase 1.
Summary of the invention
For this purpose, the present invention exactly will solve above-mentioned technical problem, to propose a kind of utilization latex enhancing immune turbidimetry
The kit of quantitatively determining human excrement pancreatic elastase 1 and its application.
In order to solve the above technical problems, the technical solution of the present invention is as follows:
The present invention provides a kind of kit for quantitative determining pancreatic elastase 1 in excrement, and the kit includes R1 examination
1 antigen calibration object solution of agent, R2 reagent and pancreatic elastase；Wherein, the R1 reagent includes electrolyte, stabilizer, surface
Activating agent, preservative and buffer；The R2 reagent includes the latex for being coated with anti-human 1 polyclonal antibody of pancreatic elastase
Grain, electrolyte, stabilizer, surfactant, preservative, buffer；The 1 antigen calibration object solution of pancreatic elastase includes
Pancreatic elastase 1 and stabilizer.
Preferably, 1 polyclonal antibody of anti-human pancreatic elastase is coupled to the latex by chemical crosslinking
Grain surface, the chemical crosslinking include the following steps:
S1, latex particle and cross-linked surface activating agent is added into crosslinking buffer solution, obtains latex particle solution；
S2, anti-human 1 polyclonal antibody of pancreatic elastase is dissolved in crosslinking buffer solution, until concentration is 1-10 μ
Mol/mL obtains anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase；
S3, the latex particle solution is mixed with the anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase, additionization
React 2-4h at room temperature after learning crosslinking agent to get the latex particle of anti-human 1 polyclonal antibody of pancreatic elastase is coated with.
Preferably, the electrolyte is at least one of sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, in reagent
Concentration be 0.1-10%.
Preferably, the stabilizer is casein, mannitol, chitosan, disodium ethylene diamine tetraacetate, bovine serum albumin
At least one of white, the concentration in reagent is 0.1-10%.
Preferably, the buffer be MES buffer, it is borate buffer, acetate buffer, phosphate buffer, sweet
One of propylhomoserin buffer, the concentration of the buffer are 10-500mmol/L, and the pH value of the buffer is 5-9.
Preferably, the surfactant be tween, fatty alcohol polyglycol ether, in polyoxyethylene phenyl ether at least
One kind, concentration of the surfactant in reagent are 0.1-10%.
Preferably, the preservative be Sodium azide, phenol, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate,
Proclin at least one of the preservatives, concentration of the preservative in reagent are 0.1-10%.
Preferably, the chemical cross-linking agent is EDC, n-hydroxysuccinimide, N- hydroxy thiosuccinimide, carbon
Change at least one of imines, hydrazides, isocyanic acid potassium；The cross-linking buffer is MES, MOPSO, MOPS, HEPES, PBS buffering
One of liquid, the pH value of the cross-linking buffer are 6-9.
Preferably, the concentration of pancreatic elastase 1 is 0.01- in the 1 antigen calibration object solution of pancreatic elastase
1500μg/mL。
The present invention also provides a kind of kits of pancreatic elastase 1 in quantitative determination excrement in detection exocrine pancreas function
Application in energy.
The above technical solution of the present invention has the following advantages over the prior art:
The kit of pancreatic elastase 1 in quantitative determination excrement of the present invention, the kit include R1 reagent,
1 antigen calibration object solution of R2 reagent and pancreatic elastase；Wherein, the R1 reagent includes electrolyte, stabilizer, surface-active
Agent, preservative and buffer；The R2 reagent includes latex particle, the electricity for being coated with anti-human 1 polyclonal antibody of pancreatic elastase
Xie Zhi, stabilizer, surfactant, preservative, buffer；The 1 antigen calibration object solution of pancreatic elastase includes pancreas bullet
Property protease 1 and stabilizer.The kit is a kind of to utilize pancreas elastin laminin in latex enhancing immune turbidimetry for Determination excrement
The device of enzyme 1, it is accurate, time saving and energy saving to have the advantages that stablize, and can combine with automatic clinical chemistry analyzer, realizes full-automatic fast
Speed measurement sample clinical detection assays effect, compared with traditional immunization is than turbid reagent, detection sensitivity improve 10 times with
On, anti-human 1 polyclonal antibody of pancreatic elastase is coupled to latex particle surface by the method being chemically crosslinked, latex particle with
By chemical bonds between antibody Fc fragment, the Percentage bound and stability of antibody are improved, be effectively protected antibody and is resisted
The active region that original combines, improves detection sensitivity.
Detailed description of the invention
In order to make the content of the present invention more clearly understood, it below according to specific embodiments of the present invention and combines
Attached drawing, the present invention is described in further detail, wherein
When Fig. 1 is the kit detection sample of pancreatic elastase 1 in quantitative determination excrement described in the embodiment of the present invention
Absorbance difference curve graph；
Fig. 2 is the kit test pancreas elasticity of pancreatic elastase 1 in quantitative determination excrement described in the embodiment of the present invention
The canonical plotting of 1 standard items of protease.
Specific embodiment
Material and source
Pancreatic elastase 1: people's pancreatic elastase 1 of purification is obtained by gene recombination technology；Other reagents are purchased from
Sigma Co., USA.
Embodiment 1
The present embodiment provides a kind of kits of pancreatic elastase 1 in quantitative determination excrement, and the kit includes R1
1 antigen calibration object solution of reagent, R2 reagent and pancreatic elastase.
Wherein, the R1 reagent is grouped as by the group matched as follows: 0.1% electrolyte, 10% stabilizer, 0.1%
Surfactant, 10% preservative, surplus be concentration 10mmol/L buffer, the electrolyte be sodium chloride, it is described
Stabilizer is casein, and the surfactant is tween, and the preservative is Sodium azide, and the buffer is MED buffer,
Its pH value is 5.
The R2 reagent is grouped as by the group matched as follows: anti-human 1 Anti-TNF-α of pancreatic elastase of the coating of 2mg/mL
The latex particle of body, 0.1% electrolyte, 10% stabilizer, 0.1% surfactant, 10% preservative, surplus are
The buffer of concentration 10mmol/L.The electrolyte is potassium chloride, and the stabilizer is mannitol, and the surfactant is rouge
Fat alcohol polyglycol ether, the preservative are phenol, and the buffer is phosphate buffer, pH value 5.
In the present embodiment, anti-human 1 polyclonal antibody of pancreatic elastase is coupled to glue by the method being chemically crosslinked
Newborn particle surface, specifically comprises the following steps:
S1, by the latex particle of 100mg in 5ml MES buffer (50mM, pH 6.0), be added cross-linked surface activating agent
Dodecyl sodium sulfate (ultimate density 0.01%), obtains latex particle solution, and the partial size of the latex particle is 50-500nm.
S2, anti-human 1 polyclonal antibody of pancreatic elastase is dissolved in 5ml MES crosslinking buffer solution (50mM, pH6.0)
In, until concentration is 1 μm of ol/mL, obtain anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase.
S3, the latex particle solution is mixed with the anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase, is added
2h is reacted at room temperature after 100mg chemical cross-linking agent 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC),
Up to the latex particle for being coated with anti-human 1 polyclonal antibody of pancreatic elastase.
The 1 antigen calibration object solution of pancreatic elastase includes pancreatic elastase 1 and stabilizer, the pancreas elasticity egg
The concentration of white enzyme 1 is 0 μ g/mL, 100 μ g/mL, 250 μ g/mL, 500 μ g/mL, 750 μ g/mL, 1500 μ g/mL, the stabilizer
For bovine serum albumin(BSA).
The pancreatic elastase 1 extracts by the following method:
1 recombination of pancreatic elastase of people is expressed by Escherichia coli fermentation, by ion exchange resin and parent
With chromatographic purifying column purification, high concentration recombined human pancreatic elastase 1 is obtained, and measures protein concentration with BCA method, for connecing
The Antibody preparation got off, while can also be used as the mother liquor of standard items and quality-control product.
1 polyclonal antibody of anti-human pancreatic elastase is prepared via a method which:
By 100 μ g pancreatic elastases 1 with equivalent Freund's complete adjuvant emulsify antigen, dorsal sc multi-point injection 2.2~
The New Zealand White Rabbit of 2.5kg emulsifies antigen every two weeks thereafter with incomplete Freund's adjuvant, carries out reinforcing exempting from method duplicate injection
Epidemic disease 3 times, amount to 4 times immune.Then from venous puncture 100ml rabbit blood, centrifugation obtains serum.Take 10ml rabbit anteserum by exempting from
Epidemic disease affinity column, it is anti-with 1000ml TBS (20mM Tris, pH 7.4,500mM NaCl, 0.05%Tween-20) buffer
Chromatographic column is rinsed again, discards efflux, after flushing, is washed with glycine/HCl (100mM, pH 2.5) buffer of 10ml
The de- antibody combined, is collected in bag filter, and be placed in 1000ml TBS (20mM Tris, pH 7.4,500mM NaCl,
0.05%Tween-20) in buffer, 4 DEG C of dialysed overnights in refrigerator obtain anti-human 1 polyclonal antibody of pancreatic elastase.
The kit of pancreatic elastase 1 can be applied to detection exocrine pancreatic function in the quantitative determination excrement.
Embodiment 2
The present embodiment provides a kind of kits of pancreatic elastase 1 in quantitative determination excrement, and the kit includes R1
1 antigen calibration object solution of reagent, R2 reagent and pancreatic elastase.
Wherein, the R1 reagent is grouped as by the group matched as follows: 10% electrolyte, 0.1% stabilizer, 10%
Surfactant, 0.1% preservative, surplus be concentration 500mmol/L buffer, the electrolyte be magnesium chloride, sulfuric acid
Magnesium compound, the two mass ratio be 1:1, the stabilizer be disodium ethylene diamine tetraacetate, bovine serum albumin(BSA) mixture, two
Person's mass ratio is 2:1, and the surfactant is the mixture of polyoxyethylene phenyl ether, tween, and the two mass ratio is 1:1, institute
The mixture that preservative is P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate is stated, the two mass ratio is 1:2, and the buffer is
Acetate buffer, pH 9.
The R2 reagent is grouped as by the group matched as follows: anti-human 1 Anti-TNF-α of pancreatic elastase of the coating of 6mg/mL
The latex particle of body, 10% electrolyte, 0.1% stabilizer, 10% surfactant, 0.1% preservative, surplus are
The buffer of concentration 500mmol/L.The electrolyte is potassium chloride, magnesium chloride mixture, and the two equivalent, the stabilizer is sweet
Reveal the mixture of pure and mild casein, the two mass ratio is 1:3, and the surfactant is polyoxyethylene phenyl ether, the anti-corrosion
Agent is Proclin series preservative, and the buffer is glycine buffer, pH value 9.
In the present embodiment, anti-human 1 polyclonal antibody of pancreatic elastase is coupled to glue by the method being chemically crosslinked
Newborn particle surface, specifically comprises the following steps:
S1, by the latex particle of 100mg in 5ml PBS buffer solution (50mM, pH 9), be added cross-linked surface activating agent ten
Dialkyl sulfonates (ultimate density 0.02%), obtain latex particle solution, and the partial size of the latex particle is 50-500nm.
S2, anti-human 1 polyclonal antibody of pancreatic elastase is dissolved in 5ml MOPSO crosslinking buffer solution (50mM,
PH9.0 in), until concentration is 10 μm of ol/mL, anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase is obtained.
S3, the latex particle solution is mixed with the anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase, is added
4h is reacted after 100mg chemical cross-linking agent n-hydroxysuccinimide at room temperature to get being coated with anti-human more than 1 gram of pancreatic elastase
The latex particle of grand antibody.
The 1 antigen calibration object solution of pancreatic elastase includes pancreatic elastase 1 and stabilizer, the pancreas elasticity egg
The concentration of white enzyme 1 is 0 μ g/mL, 50 μ g/mL, 100 μ g/mL, 250 μ g/mL, 500 μ g/mL, 750 μ g/mL, and the stabilizer is
Disodium ethylene diamine tetraacetate.Wherein pancreatic elastase 1,1 polyclonal antibody of anti-human pancreatic elastase are according to described in embodiment 1
Method preparation.
The kit of pancreatic elastase 1 can be applied to detection exocrine pancreatic function in the quantitative determination excrement.
Embodiment 3
The present embodiment provides a kind of kits of pancreatic elastase 1 in quantitative determination excrement, and the kit includes R1
1 antigen calibration object solution of reagent, R2 reagent and pancreatic elastase.
Wherein, the R1 reagent is grouped as by the group matched as follows: 3% electrolyte, 2.5% stabilizer, 5% table
Face activating agent, 0.5% preservative, surplus is the buffer of concentration 250mmol/L, and the electrolyte is sodium chloride, described steady
Determining agent is mannitol, and the surfactant is Tween-80, and the preservative is Sodium azide, and the buffer is MES buffering
Liquid, pH 7.4.
The R2 reagent is grouped as by the group matched as follows: anti-human 1 Anti-TNF-α of pancreatic elastase of the coating of 4mg/mL
The latex particle of body, 3% electrolyte, 2.5% stabilizer, 5% surfactant, 0.5% preservative, surplus are dense
Spend the buffer of 250mmol/L.The electrolyte is potassium chloride, and the stabilizer is mannitol, and the surfactant is to spit
Temperature -80, the preservative are Sodium azide, and the buffer is MES buffer, pH value 7.4.
In the present embodiment, anti-human 1 polyclonal antibody of pancreatic elastase is coupled to glue by the method being chemically crosslinked
Newborn particle surface, specifically comprises the following steps:
S1, by the latex particle of 100mg in 5ml PBS buffer solution (50mM, pH 7.2), be added cross-linked surface activating agent
Dodecyl sodium sulfate (ultimate density 0.01%), obtains latex particle solution, and the partial size of the latex particle is 50-500nm.
S2, anti-human 1 polyclonal antibody of pancreatic elastase is dissolved in 5ml HEPES crosslinking buffer solution (50mM,
PH8.0 in), until concentration is 7 μm of ol/mL, anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase is obtained.
S3, the latex particle solution is mixed with the anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase, is added
React 4h at room temperature after 100mg chemical cross-linking agent carbonization imines, hydrazides mixture to get anti-human pancreatic elastase 1 is coated with
The latex particle of polyclonal antibody.
The 1 antigen calibration object solution of pancreatic elastase includes pancreatic elastase 1 and stabilizer, the pancreas elasticity egg
The concentration of white enzyme 1 is 0 μ g/mL, 75 μ g/mL, 150 μ g/mL, 300 μ g/mL, 600 μ g/mL, 1200 μ g/mL, and the stabilizer is
The mixture of bovine serum albumin(BSA) and chitosan, the two mass ratio are 1:1.Wherein pancreatic elastase 1, anti-human pancreas elastin laminin
1 polyclonal antibody of enzyme is prepared according to method described in embodiment 1.
The kit of pancreatic elastase 1 can be applied to detection exocrine pancreatic function in the quantitative determination excrement.
Experimental example
1, the preparation of standard items
People's pancreatic elastase 1 to be purified by gene recombination technology uses bovine serum albumin gradient dilution for mother liquor
Mother liquor prepares calibration object: S5:1500 μ g/mL, S4:750 μ g/mL, S3:500 μ g/mL, S2:250 μ g/mL, S1:100 μ g/mL,
S0:0 μ g/mL.The bovine serum albumin(BSA) concentration is 1~1000 μ g/mL.
2, the preparation of quality-control product
It is dilute using bovine serum albumin gradient with people's pancreatic elastase 1 for being purified by gene recombination technology for mother liquor
Mother liquor is released, the quality-control product of two kinds of concentration: C1:300 μ g/mL, C2:110 μ g/mL is prepared.The bovine serum albumin(BSA) concentration 1~
1000μg/mL。
3, the formulation of standard curve
Dominant wavelength: 600nm, commplementary wave length: 750nm is detected using automatic clinical chemistry analyzer.
Reagent dosage: 3 μ l of sample；300 μ l of R1 reagent；100 μ l of R2 reagent.
Measuring method (Two point end assay): 3 μ l samples are added in 300 μ l R1 reagents, react 5 minutes in 37 DEG C, are then added
100 μ l R2 reagents i.e. beginning read point measures absorbance A 1,10 minutes, and read point measures absorbance A 2 again later, calculates absorbance
Difference DELTA A=A2-A1, as shown in Figure 1.
It making standard curve: using 1 standard items of pancreatic elastase of the invention, concentration is respectively S5:1500 μ g/mL,
S4:750 μ g/mL, S3:500 μ g/mL, S2:250 μ g/mL, S1:100 μ g/mL, S0:0 μ g/mL.This is measured according to above-mentioned steps
The standard curve of 1 standard items of invention pancreatic elastase.As shown in Figure 2.Each point in Fig. 2 on curve represents a content
Standard items, wherein x-axis indicates that the concentration of pancreatic elastase 1, y-axis indicate the difference of absorbance.
4, the determination of the range of linearity
By close to 1 high concentration sample of pancreatic elastase, the 1500 μ g/mL of the range of linearity upper limit, with physiological saline by 1/2,
1/4,1/8,1/16,1/32,1/64 dilution proportion is configured to the solution of 7 various concentrations altogether, separately to be free of pancreas elastin laminin
The physiological saline of enzyme 1 is as blank solution.Detect each concentration with the Two point end assay, will measurement concentration value and theoretical concentration into
Row linear regression analysis calculates regression equation are as follows: y=1.0102x-0.1321, correlation coefficient r=0.9995 show the present invention
Kit good relationship in 0~1500 μ g/mL range of linearity.
5, accuracy determination
Using kit of the invention, using automatic clinical chemistry analyzer, to 20 people, (The Third People's Hospital of Shenzhen digests
Section and clinical laboratory) carrying out excrement measurement, (>=200 μ g/mL are normal specimen, and 100-200 μ g/mL is light moderate exocrine pancreas function
Can not full sample, < 100 μ g/mL be the infull sample of severe exocrine pancreatic function).Test result is as follows shown in table 1, as a result shows
Show that the correlation of kit and clinical disease of the invention is very high.
Table 1
6, sensitivity determination
Sensitivity definition is the variation of unit concentration absorbance.With 1 calibration object of pancreatic elastase to pancreatic elastase 1
In automatic clinical chemistry analyzer upscaling, record the absorbance value that calibration object (concentration is 100 μ g/mL) is reacted with reagent is reagent
0.06, i.e. sensitivity of the reagent when calibration concentration is 100 μ g/mL is 0.06.
7, the measurement of withinrun precision
By kit measurement of the present invention with a sample 10 times, measurement mean value and withinrun precision are calculated, test result is such as
Shown in table 2, withinrun precision is 1.9% as the result is shown.
Table 2
8, anti-Interference Analysis
Chaff interferent selection is formulated and test result is as follows shown in table 3.The results show that anti-using kit of the present invention
Interference performance is good.
Table 3
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. the kit of pancreatic elastase 1 in a kind of quantitative determination excrement, which is characterized in that the kit includes R1 examination
1 antigen calibration object solution of agent, R2 reagent and pancreatic elastase；Wherein, the R1 reagent includes electrolyte, stabilizer, surface
Activating agent, preservative and buffer；The R2 reagent includes the latex for being coated with anti-human 1 polyclonal antibody of pancreatic elastase
Grain, electrolyte, stabilizer, surfactant, preservative, buffer；The 1 antigen calibration object solution of pancreatic elastase includes
Pancreatic elastase 1 and stabilizer.
2. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 1, which is characterized in that described
Anti-human 1 polyclonal antibody of pancreatic elastase is coupled to the latex particle surface, the chemical crosslinking packet by chemical crosslinking
Include following steps:
S1, latex particle and cross-linked surface activating agent is added into crosslinking buffer solution, obtains latex particle solution；
S2, anti-human 1 polyclonal antibody of pancreatic elastase is dissolved in crosslinking buffer solution, until concentration is 1-10 μm of ol/mL,
Obtain anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase；
S3, the latex particle solution is mixed with the anti-human 1 Anti-TNF-α liquid solution of pancreatic elastase, chemistry is added and hands over
2-4h is reacted after connection agent at room temperature to get the latex particle of anti-human 1 polyclonal antibody of pancreatic elastase is coated with.
3. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 1 or 2, which is characterized in that institute
Stating electrolyte is at least one of sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, and the concentration in reagent is 0.1-10%.
4. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 3, which is characterized in that described
Stabilizer is at least one of casein, mannitol, chitosan, disodium ethylene diamine tetraacetate, bovine serum albumin(BSA), in reagent
In concentration be 0.1-10%.
5. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 4, which is characterized in that described
Buffer is MES buffer, in borate buffer, acetate buffer, phosphate buffer (PBS), glycine buffer
One kind, the concentration of the buffer are 10-500mmol/L, and the pH value of the buffer is 5-9.
6. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 5, which is characterized in that described
Surfactant is at least one of tween, fatty alcohol polyglycol ether, polyoxyethylene phenyl ether, and the surfactant exists
Concentration in reagent is 0.1-10%.
7. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 6, which is characterized in that described
Preservative be Sodium azide, phenol, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate, Proclin at least one of the preservatives,
Concentration of the preservative in reagent is 0.1-10%.
8. the kit of pancreatic elastase 1 in quantitative determination excrement according to claim 2, which is characterized in that described
Chemical cross-linking agent is EDC, n-hydroxysuccinimide, N- hydroxy thiosuccinimide, be carbonized imines, hydrazides, isocyanic acid potassium
At least one of；The cross-linking buffer is one of MES, MOPSO, MOPS, HEPES, PBS buffer solution, the crosslinking
The pH value of buffer is 6-9.
9. according to the kit of pancreatic elastase 1 in the described in any item quantitative determination excrement of claim 4-8, feature exists
In in the 1 antigen calibration object solution of pancreatic elastase, the concentration of pancreatic elastase 1 is 0.01-1500 μ g/mL.
10. application of the kit of pancreatic elastase 1 in detection exocrine pancreatic function in a kind of quantitative determination excrement.
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| WO2022201056A1 (en) * | 2021-03-23 | 2022-09-29 | Kashiv Biosciences, Llc | An extraction process of pancrelipase and evaluation threof |
| CN117169519A (en) * | 2023-10-26 | 2023-12-05 | 艾康生物技术(杭州)有限公司 | Dissociation agent and kit for detecting TT3 and/or TT4 in sample |
| CN118443937A (en) * | 2024-04-03 | 2024-08-06 | 中国人民解放军海军军医大学第一附属医院 | Reagent strip and kit for detecting pancreatic elastase-1 and preparation method |
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