CN109142500B - MALDI-TOF-MS desorption ionization control method, apparatus, computer device and storage medium - Google Patents

Abstract

The application relates to a MALDI-TOF-MS desorption ionization control method, a MALDI-TOF-MS desorption ionization control device, computer equipment and a storage medium. The method comprises the following steps: receiving target point information input from the outside, and acquiring an image of a target point from MALDI-TOF-MS target plate imaging according to the target point information; processing the image of the target spot to obtain a mass spectrogram of each crystallization site in the crystallization area, and searching for an effective crystallization site in the crystallization site, wherein the signal-to-noise ratio of a sample characteristic peak of the mass spectrogram of the effective crystallization site is a preset value; and planning the path of the effective crystallization site to generate a target path, outputting the target path to external control equipment, and performing desorption ionization by the external control equipment according to the target path. The desorption ionization can be carried out on the effective crystallization sites at every time, the analysis speed and the analysis and identification efficiency are improved, and the waste of an ionization source can be avoided.

Description

MALDI-TOF-MS desorption ionization control method, device, computer equipment and storage medium

technical field

The present application relates to the field of life science technology, and in particular to a MALDI-TOF-MS (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry, matrix-assisted laser desorption ionization time of flight mass spectrometry) ionization control method, device, computer equipment and storage medium.

Background technique

MALDI-TOF-MS is a kind of soft ionization biological mass spectrometer. MALDI-TOF-MS mainly includes matrix-assisted laser desorption ionization source (MALDI) and time-of-flight mass analyzer (TOF).

The principle of MALDI is to irradiate the co-crystal film formed by the sample and the matrix with a laser. The matrix absorbs energy from the laser and transmits it to the biomolecules. During the ionization process, the protons are transferred to the biomolecules or obtained from the biomolecules, and the biomolecules are ionized. process. Therefore, it is a soft ionization technique suitable for the determination of mixtures and biological macromolecules. The principle of TOF is that the ions are accelerated to fly through the flight pipe under the action of an electric field, and are detected according to the different flight times reaching the detector. MALDI-TOF-MS has the characteristics of high sensitivity, high accuracy and high resolution. It provides a powerful analysis and testing method for life science and other fields, and plays an increasingly important role.

For example, the matrix and the polypeptide sample are placed on the sample target, the sample is ionized by laser to form a mixture of polypeptide ions, and the polypeptide ions fly in the TOF tube. The flying speed depends on the M/Z size of the polypeptide ions. The ions reaching the detector pass the detection The detector detects the M/Z of each peptide ion. After detecting the M/Z of each ion, the computer outputs the M/Z of each peptide on the same spectrum, that is, the peptide map of the protein. The peptide fingerprints were compared to identify proteins. In the traditional MALDI-TOF-MS, it is difficult to ensure that the laser is in the effective area every time in the automatic shooting mode, which will reduce the analysis and identification efficiency of the object to be measured.

SUMMARY OF THE INVENTION

Based on this, it is necessary to provide a MALDI-TOF-MS desorption ionization control method, device, computer equipment and storage medium that can improve the analysis and identification efficiency of the object to be measured, aiming at the above technical problems.

A MALDI-TOF-MS desorption ionization control method, the method comprises:

Receive externally input target target information, and obtain the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information;

processing the image of the target to obtain the mass spectrum of each crystallization site in the crystallization region;

Searching for an effective crystallization site in the crystallization site, the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value;

Path planning is performed on the effective crystallization site to generate a target path, and the target path is output to an external control device, and the external control device performs desorption and ionization according to the target path.

In one embodiment, in the method, the image of the target is processed to obtain a mass spectrum of each crystallization site in the crystallization region, including:

processing the image of the target point to obtain the spatial position coordinate domain of the crystallization area;

Obtain a mass spectrum of each crystallization site in the spatial coordinate domain of the crystallization region.

In one embodiment, in the method, the processing of the image of the target point to obtain the spatial position coordinate domain of the crystallization region includes:

extracting features of the image of the target, and identifying the features to obtain a crystallization site;

Obtain the spatial position of the crystallization site, and obtain the crystallization area corresponding to the image of the target target according to the spatial position of the crystallization site;

According to the crystallization region corresponding to the image of the target point, the spatial position coordinate domain of the crystallization region is obtained.

In one embodiment, in the method, before the receiving the externally input target target information, and before acquiring the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information, the method further includes: :

Obtain the spatial position coordinate system of the MALDI-TOF-MS target plate, and the spatial position coordinate system includes the spatial position coordinates of each target point;

The receiving the externally input target target information, and obtaining the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information, including:

receiving externally input target target point information, matching the target target point information with the spatial position coordinates of each target point, and obtaining the target target point corresponding to the target target point information in the spatial position coordinate system;

The image of the target point is obtained from the MALDI-TOF-MS target plate imaging according to the target point.

In one embodiment, in the method, the acquiring the spatial position coordinate system of the MALDI-TOF-MS target plate includes:

Obtain the imaging of MALDI-TOF-MS target plate;

Obtain the spatial position coordinates of the preset target point on the MALDI-TOF-MS target board, and use the spatial position coordinates of the preset target point as the reference target point of the spatial position coordinate system of the MALDI-TOF-MS target board coordinate;

According to the imaging of the MALDI-TOF-MS target plate and the coordinates of the reference target point, the spatial position coordinate system of the MALDI-TOF-MS target plate is obtained.

In one embodiment, in the method, obtaining the spatial position coordinate system of the MALDI-TOF-MS target plate according to the imaging of the MALDI-TOF-MS target plate and the coordinates of the reference target plate, including :

calibrating the coordinates of the reference target point according to the imaging of the MALDI-TOF-MS target plate;

According to the calibrated reference target point coordinates, the spatial position coordinates of each target point on the MALDI-TOF-MS target board are obtained, and the MALDI is established based on the spatial position coordinates of each target point on the MALDI-TOF-MS target board. - The spatial position coordinate system of the TOF-MS target plate.

In one embodiment, in the method, performing path planning on the effective crystallization site to generate a target path, comprising:

Obtaining the spatial position coordinates of each boundary crystallization site in the effective crystallization site in the spatial position coordinate system;

Linking the spatial position coordinates of each boundary crystallization site to form a target area, and obtaining a discrete map of effective crystallization sites in the target area;

Path planning is performed on the discrete graph based on a preset model to generate a target path.

A MALDI-TOF-MS desorption ionization control device, the device comprises:

a target target acquisition module for receiving externally input target target information, and obtaining an image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information;

a mass spectrum acquisition module, configured to process the image of the target to obtain a mass spectrum of each crystallization site in the crystallization region;

a crystallization site screening module, configured to search for an effective crystallization site in the crystallization site, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value;

The target path generation module is used for performing path planning on the effective crystallization site, generating a target path, and outputting the target path to an external control device, and the external control device performs desorption and ionization according to the target path.

A computer device includes a memory and a processor, the memory stores a computer program, and the processor implements the following steps when executing the computer program:

Receive externally input target target information, and obtain the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information;

processing the image of the target to obtain the mass spectrum of each crystallization site in the crystallization region;

Searching for an effective crystallization site in the crystallization site, the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value;

Path planning is performed on the effective crystallization site to generate a target path, and the target path is output to an external control device, and the external control device performs desorption and ionization according to the target path.

A computer-readable storage medium on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented:

Receive externally input target target information, and obtain the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information;

processing the image of the target to obtain the mass spectrum of each crystallization site in the crystallization region;

Searching for an effective crystallization site in the crystallization site, the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value;

Path planning is performed on the effective crystallization site to generate a target path, and the target path is output to an external control device, and the external control device performs desorption and ionization according to the target path.

The above-mentioned MALDI-TOF-MS desorption ionization control method, device, computer equipment and storage medium, by receiving externally input target target information, and obtaining the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information , and then analyze and process the image of the target site to obtain the mass spectrum of each crystallization site in the crystallization area, and find the effective crystallization site in the crystallization site. Set the value, and then plan the path of the effective crystallization site, generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path to ensure the accuracy of laser desorption and ionization. It can not only improve the analysis Speed and analysis and identification efficiency can get identification results faster; desorption and ionization according to the target path can also effectively avoid the waste of ionization sources when analyzing samples in large quantities; and the desorption and ionization objects are effective crystallization sites, which can effectively avoid Wasting a lot of time on ineffective crystallization points can greatly improve sample analysis efficiency.

Description of drawings

Fig. 1 is the application environment diagram of MALDI-TOF-MS desorption ionization control method in one embodiment;

Fig. 2 is the schematic flow sheet of MALDI-TOF-MS desorption ionization control method in one embodiment;

3 is a schematic diagram of a partition of an image of a target point in one embodiment;

4 is a schematic flowchart of a step of obtaining a coordinate domain in one embodiment;

5 is a schematic flowchart of a coordinate system acquisition step in another embodiment;

6 is a schematic diagram of image position calibration in one embodiment;

7 is a schematic diagram of the effect of the MALDI-TOF-MS desorption ionization control method in one embodiment;

8 is a structural block diagram of a MALDI-TOF-MS desorption ionization control device in one embodiment;

Figure 9 is a diagram of the internal structure of a computer device in one embodiment.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present application more clearly understood, the present application will be described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application.

The MALDI-TOF-MS desorption ionization control method provided in this application can be applied to the application environment shown in FIG. 1 . The processing device is respectively connected with the MALDI-TOF-MS and the control device, and the control device is connected with the MALDI-TOF-MS. Among them, MALDI-TOF-MS includes a vacuum system, a vacuum rapid sample changing system, an optical system, an ion source, a time-of-flight mass analyzer and a data acquisition system. Spot the sample on the target plate to form a co-crystal (including matrix), put it into the sample target holder of MALDI-TOF-MS after it is completely dry, and inject the sample; adjust the brightness of the directional focusing illumination of the target point to ensure that the sample crystal is clearly visible. The laser is emitted by the optical system, and the laser irradiates the co-crystal film formed by the sample and the matrix. The matrix absorbs the energy from the laser and transfers it to the sample molecules, so that the sample is vaporized and ionized. After being accelerated by the same electric field, the ions enter the time-of-flight mass analyzer at different speeds due to their different mass numbers, and are separated and detected. The processing device receives the externally input target target information, obtains the target target image from the MALDI-TOF-MS target plate imaging according to the target target information, analyzes and processes the target target image, and obtains each crystal position in the crystallization area. Find the effective crystallization site in the crystallization site, the sample characteristic peak signal-to-noise ratio of the mass spectrum of the effective crystallization site is a preset value, and then perform path planning for the effective crystallization site, generate the target path, and output the target The route is sent to the control device, and the desorption ionization is carried out by the control device according to the target route.

In one embodiment, as shown in FIG. 2, a MALDI-TOF-MS desorption ionization control method is provided, and the method is applied to the processing device in FIG. 1 as an example to illustrate, including the following steps:

Step 202: Receive externally input target target information, and obtain an image of the target target from the MALDI-TOF-MS target plate imaging according to the target target information.

The target point refers to the externally input target point, and the target target point information refers to the externally input target point identification, such as the target point coordinates. MALDI-TOF-MS target plate imaging refers to the image corresponding to the MALDI-TOF-MS target plate, including multiple target images. Specifically, the image of the target plate can be obtained by the camera device, and the target point can be moved to the laser exit point by controlling the movement of the three-dimensional control platform of MALDI-TOF-MS. When the target target point moves to the laser exit point, the laser exit point and the imaging The centers of the windows coincide. At this time, the target point is scanned and imaged by the camera device to obtain the image of the target point.

In step 204, the image of the target point is processed to obtain a mass spectrum of each crystallization site in the crystallization region.

After obtaining the image of the target, analyze and process the image of the target. For example, the RGB image of the target can be converted into a grayscale image by a grayscale algorithm, and then the grayscale image of the target can be extracted by a neural network algorithm. and identify the extracted image features to distinguish the outer circle, crystalline area and blank area of the target, as shown in Figure 3. Among them, the crystalline region refers to the region in which the fiber macromolecules are arranged regularly and tightly, also known as the crystalline region. The crystal region of the fiber is composed of tiny crystallites. The size of the crystallites in the crystal region is generally 1nm-10nm. The bonding force is great.

Specifically, processing the image of the target point to obtain the mass spectrum of each crystallization site in the crystallization region, including: processing the image of the target point to obtain the spatial position coordinate domain of the crystallization region; obtaining the spatial coordinates of the crystallization region Mass spectra of each crystallization site in the domain. The spatial position coordinate domain of the crystalline region includes the spatial position coordinates of each microcrystal in the crystalline region in the spatial position coordinate system of the MALDI-TOF-MS target plate. The crystallization area includes multiple crystallization sites. The crystallization site is the position of the microcrystals in the crystal region. The spatial position coordinates of each crystallization site in the spatial position coordinate system of the MALDI-TOF-MS target plate are obtained, and each crystallization site is calculated. The spatial position coordinates of the points are connected to obtain the spatial position coordinate domain of the crystalline region. The mass spectrum refers to the spectrum that ions with different mass-to-charge ratios are separated by the mass analyzer and detected by the detector and recorded.

Step 206 , searching for an effective crystallization site among the crystallization sites, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value.

The signal-to-noise ratio of the characteristic peak of the sample refers to the ratio of the spectral peak (signal response intensity) to the noise level in the mass spectrum. By controlling the equipment to desorb the crystallization sites in the ionized crystallization area one by one, analyze the mass spectrum generated after each crystallization site is clicked, for example, analyze the first 5-10 mass spectra, if the signal-to-noise ratio of the sample characteristic peak reaches the preset If required, the crystallization site is an effective crystallization site, the control device controls the laser to continue to emit, and the mass spectrum is accumulated to a specified number of times. If the signal-to-noise ratio of the characteristic peak of the sample does not meet the preset requirements, the crystallization site is determined to be an ineffective crystallization site, such as matrix crystallization, sample crystallization, etc., and the mass spectrum is no longer accumulated, and the next crystallization site is switched to analysis. This can effectively avoid wasting a lot of time on ineffective crystallization points, which can greatly improve the efficiency of sample analysis. Like other analytical instruments, the sensitivity of a mass spectrometry instrument refers to the amount of signal response produced by the amount of sample on the instrument. The sensitivity index is a reflection of the overall performance of the instrument, which is closely related to the sample, resolution, scanning speed, injection method and ionization method. The sensitivity of the mass spectrometer can be expressed in two ways: direct injection and gas chromatography injection. The direct injection method is to directly enter a standard solid sample, such as cholesterol and methyl stearate, into the ionization chamber in a certain amount, where it is vaporized and ionized, the characteristic peaks of the sample are monitored, and the number of charged ions is calculated. flow is generated. The sensitivity of the MALDI-TOF-MS instrument is based on the concentration of the sample added to the substrate, and the signal-to-noise ratio of the characteristic peaks of the mass spectrum of the sample is obtained from the instrument for measurement. The gas chromatography injection method is to inject a certain amount of sample into a GC (Gas Chromatography, gas chromatography) column, and then express the sensitivity by the signal-to-noise ratio of the characteristic peaks of the mass spectrum of the sample.

In step 208, a path planning is performed on the effective crystallization site, a target path is generated, and the target path is output to an external control device, and the external control device performs desorption and ionization according to the target path.

For example, the spatial position coordinates of each boundary crystallization site in the effective crystallization site in the spatial position coordinate system can be obtained first, and the spatial position coordinates of each boundary crystallization site can be connected to form a target area. Then, the discrete graph of each effective crystallization site in the target area is obtained, and the path planning is carried out on the discrete graph of each effective crystallization site according to the preset model. Control the external control device to desorb and ionize each crystallization site in the path one by one according to the target path. The boundary crystallization site refers to the crystallization site in the boundary position in the spatial position coordinate domain of the crystallization region, for example, it can be defined as the position away from the preset value of the horizontal and vertical coordinates in the spatial position coordinate system as the boundary position, wherein the spatial position coordinate system is the coordinate system corresponding to the spatial position coordinate domain.

The above-mentioned MALDI-TOF-MS desorption ionization control method obtains the image of the target point from the MALDI-TOF-MS target plate imaging by receiving the information of the target point input from the outside, and then analyzes the image of the target point. Perform analysis and processing to obtain a mass spectrum of desorption and ionization of each crystallization site in the crystallization area, find effective crystallization sites in the crystallization site, and the signal-to-noise ratio of the sample characteristic peaks of the mass spectrum of the effective crystallization site is a preset value, and then determine the effective crystallization site. The crystallization site carries out path planning, generates the target path, outputs the target path to the external control device, and the external control device performs desorption and ionization according to the target path to ensure the accuracy of laser desorption and ionization. It can not only improve the analysis speed and analysis and identification efficiency, but also The identification result can be obtained faster; desorption and ionization are performed according to the target path, which can effectively avoid the waste of ionization sources when analyzing samples in large quantities; and the desorption and ionization objects are effective crystallization sites, which can effectively avoid the ineffective crystallization sites. Waste a lot of time, can greatly improve the efficiency of sample analysis.

In one embodiment, as shown in FIG. 4 , in the MALDI-TOF-MS desorption ionization control method, the image of the target point is processed to obtain the spatial position coordinate domain of the crystallization area, including: Step 402 , extracting the target point The characteristics of the image are obtained, and the characteristics are identified to obtain the crystallization site; step 404, the spatial position of the crystallization site is obtained, and the crystallization area corresponding to the image of the target target is obtained according to the spatial position of the crystallization site; step 406, according to the target The crystalline area corresponding to the image of the target point is obtained, and the spatial position coordinate domain of the crystalline area is obtained.

Feature extraction refers to extracting image information to determine whether the points of each image belong to an image feature. The result of feature extraction is to divide the points on the image into different subsets. These subsets often belong to isolated points and continuous curves. or a continuous area. Extract the characteristics of the image of the target and identify it to obtain the crystallization site. For example, the grayscale-based method can be used to detect the crystallization site by using the local change of the grayscale of the image pixel. The crystallization site is based on a certain preset. Algorithmically, the pixel with the largest grayscale change in this algorithm. The position of the crystallization site can be obtained by obtaining the derivative of the grayscale around the pixel by using the differential operation. The crystallization region includes a plurality of crystallization sites. The crystallization region corresponding to the image of the target target is obtained according to the spatial position of the crystallization site, and the spatial position coordinate domain of the crystallization region is obtained according to the crystallization region corresponding to the image of the target target. Specifically, based on the spatial position coordinate system, the spatial position coordinates of each boundary crystallization site in the crystallization region corresponding to the image of the target target can be calculated respectively; and then the spatial position coordinates of each boundary crystallization site are connected to obtain the spatial position of the crystallization region. Coordinate field.

In one embodiment, the method of receiving externally input target target information, and before acquiring the image of the target target from the MALDI-TOF-MS target plate image according to the target target information, further includes: acquiring the space of the MALDI-TOF-MS target plate Position coordinate system, the spatial position coordinate system includes the spatial position coordinates of each target point; receives the target target point information input from the outside, and obtains the image of the target target point from the MALDI-TOF-MS target plate image according to the target target point information, including: Receive the externally input target target information, match the target target information with the spatial position coordinates of each target, and obtain the target target corresponding to the target target information in the spatial position coordinate system; - Acquire the image of the target target in the MS target plate image.

In the reference system, an ordered set of data selected according to a prescribed method is called a coordinate. A method of specifying coordinates in a problem is the coordinate system used for that problem. The MALDI-TOF-MS target plate is used for mass spectrometry detection of the sample, and the sample can be placed on the target plate, which can include PAC target (points 384, 96), NALDI target (points 96), AnchorChip target (points 1536, 384) and the standard 384MTP target. The target plate includes a plurality of target points, and the spatial position coordinate system of the MALDI-TOF-MS target plate is established based on the spatial position coordinates of each target point on the target plate. The spatial position coordinate system of the MALDI-TOF-MS target plate includes the spatial position coordinates of each target point. A target point on the target plate can be selected by the user as the target target point. According to the spatial position coordinates of the target target point, By controlling the movement of the three-dimensional control platform of MALDI-TOF-MS, the target point is moved to the laser exit point, and then the imaging window of the target plate can be observed through the camera device. When the target point moves to the laser exit point, the laser exit point Coincides with the center of the imaging window. At this time, the target point is scanned and imaged by the camera device to obtain the image of the target point.

In one embodiment, as shown in FIG. 5 , in the MALDI-TOF-MS desorption ionization control method, establishing a spatial position coordinate system of the MALDI-TOF-MS target plate includes: Step 502 , acquiring the MALDI-TOF-MS target plate In step 504, the spatial position coordinates of the preset target points on the MALDI-TOF-MS target board are obtained, and the spatial position coordinates of the preset target points are used as the reference target points of the spatial position coordinate system of the MALDI-TOF-MS target board. Coordinates; Step 506 , obtain the spatial position coordinate system of the MALDI-TOF-MS target plate according to the imaging of the MALDI-TOF-MS target plate and the coordinates of the reference target point.

Scan the MALDI-TOF-MS target plate with the camera to obtain the image of the MALDI-TOF-MS target plate. According to the spatial position coordinates of the preset target on the MALDI-TOF-MS target plate, the The imaging is calibrated, and the spatial position coordinates of the preset target point are used as the reference target point coordinates of the spatial position coordinate system of the MALDI-TOF-MS target plate. According to the imaging of the MALDI-TOF-MS target plate and the reference target point coordinates, MALDI is obtained. - The spatial position coordinate system of the TOF-MS target plate. Wherein, according to the imaging of the MALDI-TOF-MS target plate and the coordinates of the reference target point, the spatial position coordinate system of the MALDI-TOF-MS target plate is obtained, which may specifically include: aligning the reference target point according to the imaging of the MALDI-TOF-MS target plate The coordinates are calibrated; according to the calibrated reference target coordinates, the spatial position coordinates of each target point on the MALDI-TOF-MS target board are obtained, and the MALDI-TOF is established based on the spatial position coordinates of each target point on the MALDI-TOF-MS target board. - The spatial position coordinate system of the MS target plate. After the sample enters the target, the target plate is automatically reset to the default target point as the reference initial point of the spatial position coordinate system, and the image position is calibrated to realize the coordinateization of the spatial position of each target point, and the position of a single target point corresponds to its imaging area, such as shown in Figure 6.

In one embodiment, in the MALDI-TOF-MS desorption ionization control method, before processing the image of the target point to obtain the mass spectrum of each crystallization site in the crystallization region, the method further includes: when the image of the target point is a color image When the color image is grayscale, the color image is converted into a grayscale image; the image of the target target is processed to obtain the mass spectrum of each crystallization site in the crystallization area, including: processing the grayscale image to obtain Mass spectrum of each crystallization site in the crystalline region. The process of converting a color image into a grayscale image is called grayscale processing of the image. The color of each pixel in a color image is determined by three components of R, G, and B, and each component has 255 possible values, so that a pixel can have more than 16 million (255*255*255) color changes scope. The grayscale image is a special color image with the same three components of R, G, and B, and the variation range of one pixel point is 255, so that the calculation amount of subsequent images will become less. The description of grayscale images, like color images, still reflects the distribution and characteristics of the overall and local chromaticity and brightness levels of the entire image. The grayscale processing of an image can be realized by the following two methods. The first method is to find the average of the three components of R, G, and B for each pixel, and then assign this average to the three components of the pixel. The second method is based on the YUV color space, the physical meaning of the Y component is the brightness of the point, and the brightness level is reflected by this value. According to the relationship between the RGB and YUV color spaces, the brightness Y and R, G, and B can be established. Correspondence of color components: Y=0.3R+0.59G+0.11B, the gray value of the image is expressed by this luminance value.

In the MALDI-TOF-MS desorption ionization control method in this application, the laser output is dotted through image recognition, which can realize that each desorption ionization is within the effective area, which can improve the analysis and identification efficiency of the object to be tested. Figure 7 shows automatic blind typing and Schematic diagram of the contrast effect of image recognition dots in the present application. In the image recognition dots, path planning is performed on the effective crystallization sites, and the target paths are connected by the effective crystallization sites, and the non-effective crystallization sites are skipped.

It should be understood that although the steps in the flowcharts of FIGS. 2 , 4 and 5 are displayed in sequence according to the arrows, these steps are not necessarily executed in the sequence indicated by the arrows. Unless explicitly stated herein, the execution of these steps is not strictly limited to the order, and these steps may be performed in other orders. Moreover, at least a part of the steps in FIGS. 2 , 4 and 5 may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily executed at the same time, but may be executed at different times. These sub-steps are not necessarily completed at the same time. Alternatively, the order of execution of the stages is not necessarily sequential, but may be performed alternately or alternately with other steps or sub-steps of other steps or at least a portion of a stage.

In one embodiment, as shown in FIG. 8, a MALDI-TOF-MS desorption ionization control device is provided, including a target target acquisition module 802, a mass spectrum acquisition module 804, a crystallization site screening module 806 and a target path generation Module 808. Among them, the target target acquisition module is used to receive the target target information input from the outside, and according to the target target information, the image of the target target is obtained from the MALDI-TOF-MS target plate imaging; the mass spectrum acquisition module is used for the target target. The image of the target site is processed to obtain the mass spectrum of each crystallization site in the crystallization area; the crystallization site screening module is used to find the effective crystallization site in the crystallization site, and the sample characteristic peak signal noise of the mass spectrum of the effective crystallization site The ratio is a preset value; the target path generation module is used to plan the path of the effective crystallization site, generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path.

In one embodiment, the mass spectrum acquisition module includes a coordinate domain acquisition unit, which is used to process the image of the target to obtain the spatial position coordinate domain of the crystallization area; the mass spectrum unit is used to acquire the spatial coordinate domain of the crystallization area. Mass spectra of each crystallization site.

In one embodiment, the coordinate domain acquisition unit in the MALDI-TOF-MS desorption ionization control device includes: a feature extraction unit for extracting features of the image of the target, and identifying the features to obtain a crystallization site; a crystallization region; The acquisition unit is used to obtain the spatial position of the crystallization site, and obtain the crystallization area corresponding to the image of the target target according to the spatial position of the crystallization site; the coordinate domain unit is used to obtain the crystallization area corresponding to the image of the target target according to the crystallization area. The spatial location coordinate field of the region.

In one embodiment, the coordinate domain unit in the MALDI-TOF-MS desorption ionization control device includes: a boundary point calculation unit, configured to calculate each boundary crystallization site in the crystallization region corresponding to the image of the target target based on the spatial position coordinate system The space position coordinates of ; the boundary point connection unit is used to connect the space position coordinates of each boundary crystallization site to obtain the space position coordinate domain of the crystallization area.

In one embodiment, the target target acquisition module further includes a coordinate system acquisition module for acquiring the spatial position coordinate system of the MALDI-TOF-MS target plate, and the spatial position coordinate system includes the spatial position coordinates of each target point; The point acquisition module includes a coordinate matching unit, receives externally input target target information, matches the target target information with the spatial position coordinates of each target, and obtains the target target corresponding to the target target information in the spatial position coordinate system; The image acquisition unit is used for acquiring the image of the target point from the MALDI-TOF-MS target plate imaging according to the target point.

In one embodiment, the coordinate system acquisition module in the MALDI-TOF-MS desorption ionization control device includes: a target plate imaging acquisition unit for acquiring the image of the MALDI-TOF-MS target plate; a reference target point acquisition unit for acquiring The spatial position coordinates of the preset target point on the MALDI-TOF-MS target board are used as the reference target point coordinates of the spatial position coordinate system of the MALDI-TOF-MS target board; the coordinate system establishment unit, It is used to obtain the spatial position coordinate system of the MALDI-TOF-MS target plate according to the imaging of the MALDI-TOF-MS target plate and the coordinates of the reference target point.

In one embodiment, the coordinate system establishment unit in the MALDI-TOF-MS desorption ionization control device includes: a calibration unit for calibrating the coordinates of the reference target point according to the imaging of the MALDI-TOF-MS target plate; According to the calibrated reference target coordinates, the spatial position coordinates of each target on the MALDI-TOF-MS target plate are obtained, and the MALDI-TOF-MS target is established based on the spatial position coordinates of each target on the MALDI-TOF-MS target plate. The coordinate system of the spatial position of the board.

In one embodiment, before the mass spectrum acquisition module in the MALDI-TOF-MS desorption ionization control device further includes: a grayscale conversion module, configured to perform grayscale processing on the color image when the image of the target point is a color image, The color image is converted into a grayscale image; the mass spectrum acquisition module is used to process the grayscale image to obtain the mass spectrum of each crystallization site in the crystallization area.

In one embodiment, the target path generation module in the MALDI-TOF-MS desorption ionization control device includes: a coordinate acquisition unit for acquiring the spatial position coordinates of each boundary crystallization site in the effective crystallization site in the spatial position coordinate system; The discrete graph acquisition unit is used to connect the spatial position coordinates of each boundary crystallization site to form a target area, and obtain the discrete graph of the effective crystallization sites in the target area; the path planning unit is used to perform path planning on the discrete graph based on the preset model , to generate the target path.

For the specific definition of the MALDI-TOF-MS desorption ionization control device, reference may be made to the above definition of the MALDI-TOF-MS desorption ionization control method, which will not be repeated here. Each module in the above-mentioned MALDI-TOF-MS desorption ionization control device can be realized in whole or in part by software, hardware and combinations thereof. The above modules can be embedded in or independent of the processor in the computer device in the form of hardware, or stored in the memory in the computer device in the form of software, so that the processor can call and execute the operations corresponding to the above modules.

In one embodiment, a computer device is provided, and the computer device may be a terminal, and its internal structure diagram may be as shown in FIG. 9 . The computer equipment includes a processor, memory, a network interface, a display screen, and an input device connected by a system bus. Among them, the processor of the computer device is used to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium, an internal memory. The nonvolatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the execution of the operating system and computer programs in the non-volatile storage medium. The network interface of the computer device is used to communicate with an external terminal through a network connection. The computer program, when executed by the processor, implements a MALDI-TOF-MS desorption ionization control method. The display screen of the computer equipment may be a liquid crystal display screen or an electronic ink display screen, and the input device of the computer equipment may be a touch layer covered on the display screen, or a button, a trackball or a touchpad set on the shell of the computer equipment , or an external keyboard, trackpad, or mouse.

Those skilled in the art can understand that the structure shown in FIG. 9 is only a block diagram of a part of the structure related to the solution of the present application, and does not constitute a limitation on the computer equipment to which the solution of the present application is applied. Include more or fewer components than shown in the figures, or combine certain components, or have a different arrangement of components.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: receiving externally input target target information, according to the target target Information Obtain the image of the target from the MALDI-TOF-MS target plate imaging; process the image of the target to obtain the mass spectrum of each crystallization site in the crystallization area; find the effective crystallization site in the crystallization site, effectively The signal-to-noise ratio of the sample characteristic peaks of the mass spectrum of the crystallization site is a preset value; the path planning of the effective crystallization site is performed to generate the target path, and the target path is output to the external control device, and the external control device performs desorption and ionization according to the target path.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: receiving externally input target target information, according to the target target The information is obtained from the MALDI-TOF-MS target plate imaging to obtain the image of the target point; the image of the target point is processed to obtain the spatial position coordinate domain of the crystallization region, and the mass spectrum of each crystallization site in the spatial coordinate domain of the crystallization region is obtained. Figure; find the effective crystallization site in the crystallization site, the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; carry out the path planning for the effective crystallization site, generate the target path, and output the target path to the external control The device, which is desorbed and ionized by the external control device according to the target path.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: receiving externally input target target information, according to the target target Information Obtain the image of the target from the MALDI-TOF-MS target plate imaging; extract the features of the image of the target, identify the features, and obtain the crystallization site; obtain the spatial position of the crystallization site, according to the crystallization site obtain the crystallization area corresponding to the image of the target target; obtain the spatial coordinate domain of the crystalline area according to the crystalline area corresponding to the image of the target target; obtain the mass spectrum of each crystallization site in the spatial coordinate domain of the crystalline area , find the effective crystallization site in the crystallization site, the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; carry out the path planning for the effective crystallization site, generate the target path, and output the target path to the external control device , and the desorption ionization is carried out by the external control device according to the target path.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: receiving externally input target target information, according to the target target Information Obtain the image of the target from the MALDI-TOF-MS target plate imaging; extract the features of the image of the target, identify the features, and obtain the crystallization site; obtain the spatial position of the crystallization site, according to the crystallization site Obtain the crystallization area corresponding to the image of the target target point based on the spatial position of the target point; calculate the spatial position coordinates of each boundary crystallization site in the crystallization area corresponding to the image of the target target point based on the spatial position coordinate system; connect the spatial position of each boundary crystallization site coordinates to obtain the spatial position coordinate field of the crystallization region; obtain the mass spectrum of each crystallization site in the spatial position coordinate field of the crystallization region, find the effective crystallization site in the crystallization site, and the sample characteristic peak information of the mass spectrum of the effective crystallization site. The noise ratio is a preset value; the path planning is performed on the effective crystallization site, the target path is generated, and the target path is output to the external control device, and the external control device performs desorption and ionization according to the target path.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and when the processor executes the computer program, the processor implements the following steps: acquiring the spatial position coordinate system of the MALDI-TOF-MS target plate , the spatial position coordinate system includes the spatial position coordinates of each target point; receive the externally input target target point information, match the target target point information with the spatial position coordinates of each target point, and obtain the target target point information in the spatial position coordinate system. Corresponding target point; obtain the image of the target point from the MALDI-TOF-MS target plate imaging according to the target point; process the image of the target point to obtain the mass spectrum of each crystallization site in the crystallization area, and find the crystallization The effective crystallization site in the site, the sample characteristic peak signal-to-noise ratio of the mass spectrum of the effective crystallization site is a preset value; the path planning for the effective crystallization site is performed, the target path is generated, and the target path is output to the external control device, and the external The control device performs desorption and ionization according to the target path.

In one embodiment, a computer device is provided, comprising a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: acquiring an image of a MALDI-TOF-MS target plate, acquiring a MALDI-TOF-MS target - The spatial position coordinates of the preset target points on the TOF-MS target board, and the spatial position coordinates of the preset target points are used as the reference target point coordinates of the spatial position coordinate system of the MALDI-TOF-MS target board; according to MALDI-TOF-MS The imaging of the target plate and the coordinates of the reference target points are used to obtain the spatial position coordinate system of the MALDI-TOF-MS target plate. The spatial position coordinate system includes the spatial position coordinates of each target point; The information is matched with the spatial position coordinates of each target point to obtain the target target point corresponding to the target target point information in the spatial position coordinate system; the image of the target target point is obtained from the MALDI-TOF-MS target plate imaging according to the target target point; The image of the target is processed to obtain the mass spectrum of each crystallization site in the crystallization area, and the effective crystallization site in the crystallization site is searched, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; Carry out path planning for the effective crystallization site, generate the target path, output the target path to the external control equipment, and the external control equipment will desorb and ionize according to the target path.

In one embodiment, a computer device is provided, comprising a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: acquiring an image of a MALDI-TOF-MS target plate, acquiring a MALDI-TOF-MS target - The spatial position coordinates of the preset target points on the TOF-MS target board, and the spatial position coordinates of the preset target points are used as the reference target point coordinates of the spatial position coordinate system of the MALDI-TOF-MS target board; according to MALDI-TOF-MS The imaging of the target plate calibrates the coordinates of the reference target point; according to the calibrated reference target point coordinates, the spatial position coordinates of each target point on the MALDI-TOF-MS target plate are obtained, based on each target point on the MALDI-TOF-MS target plate The spatial position coordinate system of the MALDI-TOF-MS target board is established, and the spatial position coordinate system includes the spatial position coordinates of each target point; the target target point information input from the outside is received, and the target target point information is compared with the target point information. The spatial position coordinates are matched to obtain the target target point corresponding to the target target information in the spatial position coordinate system; the image of the target target point is obtained from the MALDI-TOF-MS target plate imaging according to the target target point; the image of the target target point is obtained. Perform processing to obtain the mass spectrum of each crystallization site in the crystallization area, find the effective crystallization site in the crystallization site, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; conduct a path for the effective crystallization site Plan, generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: receiving externally input target target information, according to the target target The information is obtained from the MALDI-TOF-MS target plate imaging to obtain the image of the target point; when the image of the target point is a color image, grayscale processing is performed on the color image, and the color image is converted into a grayscale image; Carry out processing to obtain the mass spectrum of each crystallization site in the crystallization area, find the effective crystallization site in the crystallization site, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; Path planning, generating a target path, outputting the target path to an external control device, and desorption and ionization by the external control device according to the target path.

In one embodiment, a computer device is provided, including a memory and a processor, a computer program is stored in the memory, and the processor implements the following steps when executing the computer program: receiving externally input target target information, according to the target target The information is obtained from the MALDI-TOF-MS target plate imaging to obtain the image of the target point; the image of the target point is processed to obtain the mass spectrum of each crystallization site in the crystallization area, and the effective crystallization site in the crystallization site is searched. The signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the crystallization site is a preset value; the spatial position coordinates of each boundary crystallization site in the effective crystallization site in the spatial position coordinate system are obtained; the spatial position coordinates of each boundary crystallization site are connected , form the target area, and obtain the discrete graph of the effective crystallization sites in the target area; perform path planning on the discrete graph based on the preset model, generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path. .

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: receiving externally input target target information, and obtaining target target information from MALDI according to the target target information. -Acquire the image of the target in the TOF-MS target plate imaging; process the image of the target to obtain the mass spectrum of each crystallization site in the crystallization area; find the effective crystallization site and effective crystallization site in the crystallization site The signal-to-noise ratio of the sample characteristic peaks of the mass spectrogram is a preset value; the path planning is performed on the effective crystallization site, the target path is generated, and the target path is output to the external control device, and the external control device performs desorption and ionization according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: receiving externally input target target information, and obtaining target target information from MALDI according to the target target information. -Acquire the image of the target point in the TOF-MS target plate imaging; process the image of the target point to obtain the spatial position coordinate domain of the crystallization area, and obtain the mass spectrum of each crystallization site in the spatial coordinate domain of the crystallization area; search The signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site and the effective crystallization site is a preset value; the path planning for the effective crystallization site is performed, the target path is generated, and the target path is output to the external control device, which is controlled by The external control device performs desorption and ionization according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: receiving externally input target target information, and obtaining target target information from MALDI according to the target target information. - Obtain the image of the target in the TOF-MS target plate imaging; extract the features of the image of the target, identify the features, and obtain the crystallization site; obtain the spatial position of the crystallization site, according to the spatial position of the crystallization site Obtain the crystallization area corresponding to the image of the target point; obtain the spatial position coordinate domain of the crystallization area according to the crystallization area corresponding to the image of the target target point; The effective crystallization site in the site, the sample characteristic peak signal-to-noise ratio of the mass spectrum of the effective crystallization site is a preset value; the path planning for the effective crystallization site is performed, the target path is generated, and the target path is output to the external control device, and the external The control device performs desorption and ionization according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: receiving externally input target target information, and obtaining target target information from MALDI according to the target target information. - Obtain the image of the target in the TOF-MS target plate imaging; extract the features of the image of the target, identify the features, and obtain the crystallization site; obtain the spatial position of the crystallization site, according to the spatial position of the crystallization site Obtain the crystallization area corresponding to the image of the target target; based on the spatial position coordinate system, calculate the spatial position coordinates of each boundary crystallization site in the crystallization area corresponding to the image of the target target; connect the spatial position coordinates of each boundary crystallization site to obtain The spatial position coordinate field of the crystallization region; the mass spectrum of each crystallization site in the spatial position coordinate field of the crystallization region is obtained, and the effective crystallization site is found in the crystallization site. The sample characteristic peak signal-to-noise ratio of the mass spectrum of the effective crystallization site is Preset value; carry out path planning for the effective crystallization site, generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: acquiring the spatial position coordinate system of the MALDI-TOF-MS target plate, the spatial position The coordinate system includes the spatial position coordinates of each target point; receives the target target information input from the outside, matches the target target information with the spatial position coordinates of each target point, and obtains the target corresponding to the target target information in the spatial position coordinate system. Target; obtain the image of the target from the MALDI-TOF-MS target plate imaging according to the target; process the image of the target to obtain the mass spectrum of each crystallization site in the crystallization area, and find the The effective crystallization site, the sample characteristic peak signal-to-noise ratio of the mass spectrum of the effective crystallization site is a preset value; the path planning for the effective crystallization site is performed, the target path is generated, and the target path is output to the external control device. The target path is desorbed and ionized.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, realizes the following steps: acquiring an image of a MALDI-TOF-MS target plate, acquiring a MALDI-TOF- The spatial position coordinates of the preset target point on the MS target plate are used as the reference target point coordinates of the spatial position coordinate system of the MALDI-TOF-MS target plate; Image and refer to the coordinates of the target points to obtain the spatial position coordinate system of the MALDI-TOF-MS target plate, which includes the spatial position coordinates of each target point; The spatial position coordinates of the target points are matched to obtain the target target points corresponding to the target target information in the spatial position coordinate system; the images of the target target points are obtained from the MALDI-TOF-MS target plate imaging according to the target target points; The image of the crystallization point is processed to obtain the mass spectrum of each crystallization site in the crystallization area, and the effective crystallization site in the crystallization site is searched. The signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; The site performs path planning, generates a target path, outputs the target path to an external control device, and the external control device performs desorption and ionization according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, realizes the following steps: acquiring an image of a MALDI-TOF-MS target plate, acquiring a MALDI-TOF- The spatial position coordinates of the preset target point on the MS target plate are used as the reference target point coordinates of the spatial position coordinate system of the MALDI-TOF-MS target plate; The imaging calibrates the coordinates of the reference target; according to the calibrated coordinates of the reference target, the spatial position coordinates of each target on the MALDI-TOF-MS target plate are obtained, based on the spatial position of each target on the MALDI-TOF-MS target plate The coordinates establish the spatial position coordinate system of the MALDI-TOF-MS target board, and the spatial position coordinate system includes the spatial position coordinates of each target point; receives the externally input target target point information, and compares the target target point information with the spatial position coordinates of each target point. Carry out matching to obtain the target target corresponding to the target target information in the spatial position coordinate system; obtain the image of the target target from the MALDI-TOF-MS target plate imaging according to the target target; process the image of the target target, Obtain the mass spectrum of each crystallization site in the crystallization area, find the effective crystallization site in the crystallization site, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; perform path planning on the effective crystallization site, generate Target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: receiving externally input target target information, and obtaining target target information from MALDI according to the target target information. -In the TOF-MS target plate imaging, the image of the target point is obtained; when the image of the target point is a color image, grayscale processing is performed on the color image, and the color image is converted into a grayscale image; after processing the grayscale image, Obtain the mass spectrum of each crystallization site in the crystallization area, find the effective crystallization site in the crystallization site, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum of the effective crystallization site is a preset value; perform path planning for the effective crystallization site, Generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path.

In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored, and when the computer program is executed by a processor, the following steps are implemented: receiving externally input target target information, and obtaining target target information from MALDI according to the target target information. -Acquire the image of the target in the TOF-MS target plate imaging; process the image of the target to obtain the mass spectrum of each crystallization site in the crystallization area, and find the effective crystallization site and effective crystallization site in the crystallization site The signal-to-noise ratio of the sample characteristic peaks of the mass spectrogram is a preset value; the spatial position coordinates of each boundary crystallization site in the effective crystallization site in the spatial position coordinate system are obtained; the spatial position coordinates of each boundary crystallization site are combined to form a target area, obtain the discrete graph of effective crystallization sites in the target area; perform path planning on the discrete graph based on the preset model, generate the target path, output the target path to the external control device, and the external control device will desorb and ionize according to the target path.

Those of ordinary skill in the art can understand that all or part of the processes in the methods of the above embodiments can be implemented by instructing relevant hardware through a computer program, and the computer program can be stored in a non-volatile computer-readable storage In the medium, when the computer program is executed, it may include the processes of the above-mentioned method embodiments. Wherein, any reference to memory, storage, database or other medium used in the various embodiments provided in this application may include non-volatile and/or volatile memory. Nonvolatile memory may include read only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in various forms such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous chain Road (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.

The technical features of the above embodiments can be combined arbitrarily. In order to make the description simple, all possible combinations of the technical features in the above embodiments are not described. However, as long as there is no contradiction in the combination of these technical features It is considered to be the range described in this specification.

The above-mentioned embodiments only represent several embodiments of the present application, and the descriptions thereof are specific and detailed, but should not be construed as a limitation on the scope of the invention patent. It should be pointed out that for those skilled in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the scope of protection of the patent of the present application shall be subject to the appended claims.

Claims (10)

1. A MALDI-TOF-MS desorption ionization control method, the method comprising:

receiving target point information input from the outside, and acquiring an image of the target point from MALDI-TOF-MS target plate imaging according to the target point information;

processing the image of the target spot to obtain a mass spectrogram of each crystallization site in a crystallization area;

searching effective crystallization sites in the crystallization sites, wherein the signal-to-noise ratio of sample characteristic peaks of mass spectrograms of the effective crystallization sites is a preset value;

and planning a path of the effective crystallization site to generate a target path, outputting the target path to external control equipment, and carrying out desorption ionization by the external control equipment according to the target path.

2. The method of claim 1, wherein the processing the image of the target point to obtain a mass spectrogram of each crystallization site in the crystallization region comprises:

processing the image of the target spot to obtain a spatial position coordinate domain of a crystallization area;

and acquiring a mass spectrogram of each crystallization site in the space coordinate domain of the crystallization region.

3. The method according to claim 2, wherein the processing the image of the target point to obtain a spatial position coordinate domain of the crystalline region comprises:

extracting the characteristics of the image of the target spot, and identifying the characteristics to obtain a crystallization site;

acquiring the spatial position of the crystallization site, and acquiring a crystallization area corresponding to the image of the target spot according to the spatial position of the crystallization site;

and obtaining a spatial position coordinate domain of the crystallization region according to the crystallization region corresponding to the image of the target point.

4. The method according to claim 1, wherein the receiving of the externally input target point information further comprises, before acquiring the image of the target point from the MALDI-TOF-MS target plate imaging based on the target point information:

acquiring a space position coordinate system of the MALDI-TOF-MS target plate, wherein the space position coordinate system comprises space position coordinates of each target point;

the receiving of externally input target point information and the obtaining of the target point image from MALDI-TOF-MS target plate imaging according to the target point information include:

receiving target point information input from the outside, and matching the target point information with the spatial position coordinates of each target point to obtain a corresponding target point of the target point information in the spatial position coordinate system;

and acquiring an image of the target point from MALDI-TOF-MS target plate imaging according to the target point.

5. The method according to claim 4, wherein acquiring a spatial position coordinate system of the MALDI-TOF-MS target plate comprises:

acquiring an image of the MALDI-TOF-MS target plate;

acquiring the space position coordinate of a preset target point on the MALDI-TOF-MS target plate, and taking the space position coordinate of the preset target point as the reference target point coordinate of the space position coordinate system of the MALDI-TOF-MS target plate;

and obtaining a space position coordinate system of the MALDI-TOF-MS target plate according to the imaging of the MALDI-TOF-MS target plate and the reference target point coordinate.

6. The method according to claim 5, wherein obtaining the spatial position coordinate system of the MALDI-TOF-MS target plate from the imaging of the MALDI-TOF-MS target plate and the reference target coordinates comprises:

calibrating the reference target coordinates based on the imaging of the MALDI-TOF-MS target plate;

and obtaining the space position coordinates of each target point on the MALDI-TOF-MS target plate according to the calibrated reference target point coordinates, and establishing a space position coordinate system of the MALDI-TOF-MS target plate based on the space position coordinates of each target point on the MALDI-TOF-MS target plate.

7. The method of claim 4, wherein the path planning the effective crystallization site to generate a target path comprises:

acquiring the space position coordinates of each boundary crystallization site in the effective crystallization sites in the space position coordinate system;

connecting the spatial position coordinates of the boundary crystallization sites to form a target area, and acquiring a discrete graph of effective crystallization sites in the target area;

and planning the path of the discrete graph based on a preset model to generate a target path.

8. A MALDI-TOF-MS desorption ionization control apparatus, comprising:

the target point acquisition module is used for receiving target point information input from the outside and acquiring an image of the target point from MALDI-TOF-MS target plate imaging according to the target point information;

the mass spectrogram acquisition module is used for processing the image of the target spot to obtain a mass spectrogram of each crystallization site in the crystallization area;

the crystallization site screening module is used for searching for effective crystallization sites in the crystallization sites, and the signal-to-noise ratio of sample characteristic peaks of mass spectrograms of the effective crystallization sites is a preset value;

and the target path generation module is used for carrying out path planning on the effective crystallization sites, generating a target path, outputting the target path to external control equipment, and carrying out desorption ionization by the external control equipment according to the target path.

9. A computer device comprising a memory and a processor, the memory storing a computer program, wherein the processor implements the steps of the method of any one of claims 1 to 7 when executing the computer program.

10. A computer-readable storage medium, on which a computer program is stored, which, when being executed by a processor, carries out the steps of the method of any one of claims 1 to 7.

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