CN109073622A

CN109073622A - The method and apparatus of the high production test of processing for the one or more features to plant

Info

Publication number: CN109073622A
Application number: CN201780027392.2A
Authority: CN
Inventors: F·莱恩斯; J·埃克豪特
Original assignee: BASF Plant Science Co GmbH
Current assignee: BASF Plant Science Co GmbH
Priority date: 2016-05-06
Filing date: 2017-04-28
Publication date: 2018-12-21
Also published as: WO2017191065A1; CA3023336A1; EP3452823A1; AU2017259723A1

Abstract

The present invention relates to a kind of methods and apparatus for assessing the effect of the processing to the one or more features of plant.More particularly it relates to a kind of method and apparatus for the effect for analyzing the processing to the one or more features of plant for high production.

Description

Processing for the one or more features to plant high production test method and Equipment

Technical field

The present invention relates to the assessments of the effect of the processing of the one or more features to plant.

More particularly it relates to a kind of for assessing the recruitment evaluation of the processing to the one or more features of plant Method and apparatus.

Background technique

Make plant growth, for example, in order to they seed (also referred to as seed crop) and cultivated plant --- such as water Rice, wheat, barley, corn and soybean, rape, sunflower, millet and safflower --- when, a main target is to support plant Growth, so that their seed or biomass or the yield of root are high.Peasant particularly by application fertilizer, herbicide, pesticide, kill Worm agent, fungicide, nematicide and/or fungicide support this growth.

In exploitation, these are used for the chemicals of application and/or biological products such as fertilizer, herbicide, pesticide, insecticide, kill When microbial inoculum, nematicide and/or fungicide, the test of these products and the test of the preparation comprising these products are a weights Want step.The application effect and its effective quantity for screening these products are carried out in the field conditions using a large amount of plants 's.Developed the acceleration system for screening herbicide treatment, such as such as Stanley et al. in Forests 2014,5, Described in 1584-1595.

For quickly, accurately and effectively to screen to the tool of the effect of the chemistry and/or biological treatment of plant be plant Cultivate the necessity of industry.But other types of processing may also provide further opinion for plant culture industry.

The conventional method of effect for assessing the processing to plant includes the phenotypic analysis of plant, is related to labour intensive Type program, such as the manual and visual measurement of size, such as biomass, pigment, shape, growth, plant parts above and below the ground Counting and for example single leaf of plant parts, inflorescence and seed weigh.A part of these operations is needed from opposite plant Interested plant parts are separated in sundries official.The progress of Plant phenotypic analysis can obtain, such as WO 2010/ Described in 031780 or WO2013/001436.

Summary of the invention

Therefore, it is an object of the present invention to provide one kind at least partly avoid the system learnt by the prior art and The shortcomings that method and insufficient device and method.

The present invention by provide it is a kind of for assess the device and method of the effect of the processing to the physical characteristic of plant come Overcome these disadvantages.In a preferred embodiment, which provides the place to the one or more features of plant The high production of the effect of reason and full-automatic assessment.

The invention further relates to a kind of for being selected most based on the scoring of the one or more features to processed plant The method and apparatus of ideal genotype and a kind of method for quickly analyzing the resistance of processed plant sample. For example, biotic stress can be caused by bacterium, fungi or virus disease, insect and nematode.For example, abiotic stress can be by Heat, arid, cold, wind, high salinity and low or excessively high trophic level cause.The invention further relates to chemistry needed for a kind of screening or Biologic artifact is to use itself or its preparation to handle plant or for example determine this chemistry or life according to the stage of development of plant The method and apparatus of the best application mechanism of compounds.The example of chemical compound includes fertilizer, herbicide, insecticide, removes Evil agent, fungicide, nematicide, induction or the compound and nutrient that inhibit certain development steps in plant growth；It is raw The example of compounds includes the preparation containing microorganism or spore, is had to plant growth specific, preferred beneficial to effect Fruit (growth promotes, disease treatment).

The device and method of the type can apply in all agricultural researches and business activity field and all and plant In object and the related chemistry of plant sample and/or field of biotechnology.Preferably, apparatus and method according to the invention can answer For the test of the test of plant, chemistry or biologic artifact and/or the technical field of the method testing for handling plant In, the test is, for example, one or more of following test: the test of the foliage applying of biological products and/or chemicals And/or assessment；Test of the processed plant to the resistance of specific type adverse circumstance；Specific fertilizer, insecticide, is removed the evil at herbicide Agent, the test of fungicide, nematicide and/or nutrient；The test of the effect and/or validity of particular treatment, such as 1 is carried out to plant or plant sample using fertilizer and/or biocide, 2,3,4,5,6,7,8,9,10,11,12,13,14,15, 16,17,18,19,20 processing.However, other application of the invention is possible.

Herein, we describe a kind of methods for analyzing the effect of the processing to the one or more features of plant And equipment, assessment is significantly improved compared with the conventional automated method based on " first-in last-out " or " first in first out " principle Statistical power, because it provides the true randomization of plant, to reduce orientation and/or edge effect (i.e. growth place Specific position caused by influence to plant growth).Method described herein and equipment realize the high production analysis of plant, Because these methods and apparatus realize Continuous maching and the processing of provided plant.Preferably, it method of the invention and sets Standby includes full-automatic imaging and image processing step.

More advantages of the invention will become obvious from being described below.

It can be by means of the present invention with the plant of equipment evaluation with similar or different gene information Any plant or group.These plants can be the hybridization, inbreeding and genetically modified plants of different cultivars.

One aspect of the present invention provides a kind of method for high production assessment to the effect of the processing of plurality of plants.

Method includes the following steps: providing at least one plant of plant in the multiple container for plants wherein grown, and it is used for The container moving system of the mobile container for plants.It provides and handles preceding randomization system to be randomized the suitable of the container for plants Sequence.Additionally provide randomization system after a kind of processing system and a kind of processing.The container moving system is by each plant Container is moved to randomization system before processing, is random in the sequence of wherein container for plants.Hereafter, container moving system will hold Device is moved to processing system, and then processing system provides at least single treatment.Hereafter, container moving system is mobile everywhere by container Randomization system after reason is randomized for the second time in the sequence of wherein container for plants.Hereafter, container moving system is mobile by container To growth position.Randomisation step ensures the orientation or edge of the growth position of the group from processed plant after processing Effect minimizes.Then one or more time points after the treatment are assessed in the next step described is handled at least once Effect.

Processing in the above method can be any processing that plant can undergo.This processing can be in following processing One of or more persons: foliar treatment, for example, carried out using chemistry or biological solution or powder foliar treatment, use coating solution Or the powder foliar treatment carried out, the foliar treatment that uses label solution or powder to carry out；With standard solution (nutrient solution and/or Biocide solution) it pours；Heat and/or cool the plant in container for plants；Illumination is provided；Shake container for plants；To plant Apply blowing；Rain and/or snow and/or hail apply；High pressure or low pressure atmospheric environment apply；Sootiness or gas apply；Or sound Or sound wave processing.Other processing are also possible to pollination or hormone and apply, or inoculation and/or insect and/or microorganism and/ Or fungal infection applies.

Plant in greenhouse is by growing environment caused by the variation supplied as such as temperature, humidity, light, nutrition and water Variation influences, and the variation depends on the position of plant in the greenhouse.The deep plant in greenhouse is exposed to and greenhouse center The different micro of plant.In general, plant is placed on the desk in rows of container for plants or in this greenhouse, and And another place by the way that container for plants to be moved in greenhouse is handled and the phenotype of environmental correclation and/or metabolin ingredient The problem of.In the case where placement in a row, most commercial system is worked with first in first out or first-in last-out.This cannot be mentioned For the true randomization of plant or container for plants.System and method through the invention overcome this point.Method of the invention And the advantages of equipment, is, randomization use original culture position as randomization factor/factor before handling, and after processing with Machineization uses the processing as randomization factor/factor.The first time is randomized the shadow of the growth position before being averaged processing It rings, and second of randomization after processing will be such that processed plant is randomized again, so that subsequent to one or more The measurement of feature will carry out on the plant of true random, these plants also have its specific micro after the treatment Cultivation position growth.In addition, in growth heterologous plant group (for example, plant group, mutagenic treatment with different genotype A series of afterwards or after transgenic events plant group) in the case where, it can be further randomized sowing or planting the stage, To minimize azimuthal effect or edge effect from growth position.

It is preferred that the plurality of plants (district's groups) for having carried out same treatment to one group are handled.Therefore, method of the invention mentions Supplied a kind of fully automatic system to carry out randomized block experiment, with test the effect of particular procedure or identify one group of processing it In for expectation or unexpected effect optimal representation processing；Such as the leaf of the same substance progress using different dilutions Face application.

In a preferred embodiment, method of the invention is the high production of foliar treatment, the effect preferably sprayed Assessment comprising step as provided.In a more preferred embodiment, then this method also provides dry system System, wherein container for plants passes through the drying system after sprinkling processing.In another preferred embodiment of the present, processing is with regulation Solution (nutrient solution as defined in such as or defined biocide) irrigating plant container.

Preferably, each plant is all associated with unique identifier.Preferably, identifier can be or may include but not It is limited to one or more of symbol identified below: bar code；Contactless electronic identifier, that is, including can be preferably in reading machine Between structure, preferably reader and identifier without any physical contact in the case where from identifier read at least one of information Identifier, most preferably, identifier can be at least one RFID tag (RFID tag).It is alternatively, however, or attached Add ground, other types of identifier is possible.The information can be simple mark, such as plant sample and/or genotype And/or growth conditions and/or processing.It is connect in general, at least one described identifier not necessarily must carry out physics with plant Touching, but should by it is any it is specific in a manner of be assigned to corresponding plant.

Assessment can be completed by visual score or by sampling or being imaged.

Preferably, this method also provides at least one imaging system.While executing this method, container for plants is also passed through The imaging system.Imaging can be completed before treatment and/or later.Preferably, it is handled using the imaging system The assessment of effect.The assessment of the effect of this processing preferably completion in the effect that plant has certain time to obtain complete process. In the case where foliage applying, preferably have an opportunity to be assessed after growth position is with random district's groups pattern growth in plant. It may then pass through using the imaging system of this method and plant be imaged.

Preferably, imaging system includes at least one detector.

The term " detector " being such as used in the present invention can refer to any kind of detector, be preferably used for electromagnetic wave Detector.The term " electromagnetic radiation " being such as used in the present invention may include visible light, X-ray, UV, infrared and near-infrared, Heat and terahertz emission.It may include monochromatic electromagnetism (EM) radiation and wide spectrum EM radiation, and may include discontinuous EM spoke It penetrates and link up EM radiation, polarization and non-polarized EM is radiated.Other types of electromagnetic wave is also possible.It is highly preferred that detection Device may include for the light at least one visible, infrared and ultraviolet wavelength region spectral wavelength region detector and Most preferably video camera/camera.Video camera/the camera can be the preferably number with space and/or temporal resolution Word video camera/camera.

Preferably, at least one spatial resolution images of detector acquisition.Then it is surveyed by software appropriate from described image One or more features are measured, then the software provides result information.

Preferably, imaging system is that the one or more features of each plant are imaged.Then, imaging system also mentions The image of the one or more features of each plant is analyzed for use by computer disposal, and respectively by result information and every plant of plant The unique identifier information of object is associated.

In a more preferred embodiment, this method further includes that electromagnetic wave is directed to plant so that the plant The step of transmitting or reflection electromagnetic wave.Then it will be planted under the different wave length for wherein obtaining the image including pixel by detector Object imaging.Based on the image alignment that pixel records these at different wavelengths, so that generating 3-D image.3-D image As cube includes 2 Spatial Dimensions and 1 spectral Dimensions.In the next step of this method, in conjunction with the weighting of different wave length The conventional mathematical prediction model of contribution is used to obtain the multispectral or Hyper spectral Imaging cube of plant, and then by suitable Software from the imaging one or more of features of cube bulk measurement.

The electromagnetic wave for emitting from plant or reflecting is preferably transmitted light.In another preferred embodiment of the present, it is sent out from plant The electromagnetic wave penetrated or reflected is reflected light.

In a preferred embodiment, in the near infrared range of spectrum (preferably between 900 and 1800nm) Image is collected under many different narrow-bands.

In a more preferred embodiment, this method includes the above combination 3D imaging ultraphotic spectrum or mostly light Spectrum imaging provides 4 dimension images.

Term " image " as used in the present invention can refer to any kind of image, preferably at least two dimensional image.Institute Stating image can be optical imagery.Image may include transmission image and/or shadow image and/or reflected image.Inspection can be passed through Transmitting signal --- such as fluorescence and/or phosphorescent signal --- is surveyed to generate image.Therefore, it can be measured by chlorophyll fluorescence And/or selectable marker fluorescence measurement generates image.Can be used to generate the signal of image, to can be the time upper discrete or can To be continuous signal.Other types of image is also possible, such as example described below.From the above, it is seen that as in the present invention The term " imaging " used can refer to any mode for obtaining image.It is one or more from image measurement by software appropriate A feature.If desired, algorithm can be used to assess the one or more features of measurement.

In a preferred embodiment, imaging system is moved simultaneously and is rotated so as to take in a controlled manner in plant Plant is imaged when obtaining the image of all sides of plant, and stores them in a digital format, such as such as institute in WO2010/031780 It states.By rotating plant while plant is imaged, can be filled to avoid plant is oriented its maximum radial axis towards imaging The additional step set, and obtain the more complete image of plant.

One or more of features include but is not limited to one or more of following: the physical table of the observable of plant Now, it phenotypic character, metabolic trait, color, green degree, yield, growth, biomass, maturity, transgene traits, blooms, nutrient Use, the use of water or the influence of disease, pest and/or adverse circumstance.Preferably, one or more of features include area, Highly, the presence or absence of width, Ye Jiao, the number of sheets, inflorescence and/or one or more of quantity, branch number and branching pattern/pattern. In another preferred embodiment of the present, one or more of features include one or more different metabolins, and may be needed Assess the presence or absence of specific metabolite, the quantity of metabolin, amount of specific metabolite, etc..

In a preferred embodiment, method of the invention can be used for detecting can measuring by being imaged for plant Any feature.Image can be obtained from aerial plant position and/or plant root.Aerial plant position can be branch, leaf, divide One or more of tiller, inflorescence, flower, seed.In a preferred embodiment, one or more of features are quantitative One or more of shape, biochemical trait and morphological characters.In a more preferred, biochemical trait is selected from oily group Divide, protein component, carbohydrate ingredient, fibre fractionation, oil content, protein content, carbohydrate content, starch contain The group that amount, fiber content, dry weight and water content form.In another more preferred embodiment, morphological characters, which is selected from, plants Rack structure, plant size, plant shape, ground biomass, vegetation color, plant growth rate, leaf surface texture, plant weight Amount, plant integrality, leaf integrality, leaf color, leaf shape, leaf size, leaf growth rate, underground biomass, root growth rate, Root thickness, root long degree, root anchoring, inflorescence framework, flower size, flower-shape shape, flower color, flower surface texture, flower weight, use up it is whole Property, endosperm size, embryo size, shape of the seed, seed sizes, seed color, the surface of the seed texture, seed weight, seed are close Degree and seed integrity.As used herein, integrality with disease, insect pest are infected, in fungal infection or environmental stress appoint The neurological susceptibility or resistance of what one are related.In an alternative preferred embodiment, quantitative character is selected from (green) leaf Amount, the amount of root (such as amount of hairy and/or branch root), the amount of little Hua, the amount of seed, the amount of deaf-seed, branch amount, seed Weight, seed weight and/or full rate.However, other types of parameter and/or the parameter referred to and/or other parameters Combination is possible, such as: the ground biomass of each plant and unit area；The underground biomass of unit area；It is raw on the ground The content of oil, starch and/or protein in object amount (such as seed or nutrition position of plant)；The flower (little Hua) of each plant Number；Or the framework of improvement, such as increased stem diameter, thickness or physical property (such as the soil permeability energy of the elasticity of root, root Power) improvement.Under applicable circumstances, can over time or by with suitable check plant (such as wild type or base Quasi- plant or untreated plant) it is compared to the variation of measurement plant characteristics.Untreated plant for example can be The plant being not handled by completely, or in the case where sprinkling only with water or not active constituent preparation processing plant. Those skilled in the art will know that selecting suitable check plant.

In another preferred embodiment of the present, what method of the invention can be used for detecting plant can be by obtaining plant Small sample is come any feature for measuring.Sample can be obtained from any position of plant, such as ground position, such as leaf or flower, Or it is obtained from underground position, such as root or root microorganism group.Then the one or more for analyzing sample by metabolic profile is special Sign.

In a preferred embodiment, this method further includes analyzing the one or more features of one plant or plurality of plants The step of result information is to determine the influence handled.

The influence that method of the invention can be used for analyzing gene modification to plant in conjunction with the assessment of the effect to processing, it is special It is not the influence to the one or more features of plant, and selects the plant with interested gene modification.This method packet Include following steps: firstly, culture has the plurality of plants of different genotype.Preferably, by each plant and identifier --- more Preferably, by the plant and machine-readable identification symbol that other plants distinguish --- it is associated.Handle plant and using herein The method of description obtains image or sample, then for one or more features as described above and/or character analysis these figures Picture or sample, to determine influence of the processing to gene modification.It then can be to about the interested gene modification of processing Plant or its seed are selected.If desired, algorithm can be used to select and assess the one or more features of measurement, and And statistic analysis result to be to identify the plant with interested gene modification, for selecting the candidate put up the best performance or selection Given the candidate with any given feature is further processed to any.

The formation of genotypic variation can be based on the gene modification completed in the lab, but also may rely on and pass through skill Art obtain genetic change generation, the technology include by conventional hybridization recombination, chemical mutagenesis, radiation-induced mutation, Somatic hybridization, interspecific hybridization and genetic engineering.It can be by the plant of acquisition and other non-transgenic plants, genetically modified plants And/or corresponding check plant is compared.After forming genotypic variation, execute to most desired one or more The selection of those of agronomic characteristics genotype.

By by suitable software from image or sample measure information that one or more features obtain preferably also with mark It is associated to know symbol.

The present invention provides a kind of for assessing and recording to the one or more features of plant in its another aspect The method of the effect of processing, method includes the following steps: using method described herein identify plant, handle and by plant at Picture determines one or more features, and result is recorded in computer data together with plant identifier with prescribed form In library.

The Computer Database worked out and being handled as described above plant can be asked and can be quick Compare the feature from a large amount of different plants, and the seed for therefore allowing quick determination that can therefrom derive output has expectation The seed of the other plant of feature.

The present invention provides a method that this method is used for the plant in the processed plant of a batch in its another aspect The one or more features of object are compared with the individual features for each batch of plant for having carried out another processing, wherein by each batch Plant carries out working out Computer Database according to the processing of the last period, but inquires this about one or more of features Computer Database.

Equally, the Computer Database worked out and being handled as described above plant can be asked and can The quickly feature relatively from a large amount of different plants, and therefore allow quickly to determine and can be used to handle plant and there is required spy The compound of sign.These compounds can be chemical compound or biologic artifact.In a similar way, computer can be inquired For database to determine optimal dose or treatment mechanism, this depends on how the processing of setting plant.Therefore, the present invention is another at its It is provided a method in aspect, this method is used to compare with each batch of untreated plant or carry out the plant of another processing Individual features compare on the influential one or more chemical compounds of the feature of plant in the processed plant of a batch or Biologic artifact.

Preferably, this method additionally provides a step, wherein one plant of selection or plurality of plants are to be further used for plant In breeding or Promoting Experiment or for introducing further modification in transgenic plants.Similarly, this method additionally provides one A step wherein selecting one or more compounds to be further used in Promoting Experiment, or has wherein advanced optimized meter Amount or treatment mechanism.The example of Promoting Experiment includes further optimization experiment, or further choice experiment, further sieve Choosing experiment etc..

Another aspect provides a kind of apply for high production just wherein to grow at least one plant of plant The equipment of the processing of multiple container for plants, wherein the equipment includes: the container moving system for moving container for plants；For with Randomization system before the processing of the sequence of machine container for plants；Processing system；With randomization system after processing.Each container for plants It is moved in equipment by container moving system and is moved to randomization system before processing.Then preceding randomization is through this process Container for plants is randomized by system, wherein in container after the enterprising moved further to processing system of container moving system, processing system Then system handles the plant in container for plants.Hereafter, container for plants is moved to after processing to be randomized via container moving system is System, randomization system executes second of randomization of container for plants after the processing.Hereafter, plant is held by container moving system Device removes from the device.Preferably, container for plants is then moved to plant growth position, such as greenhouse or screening room.

Processing in above equipment can be any processing that plant can undergo.This processing can be in following processing One of or more persons: foliar treatment；It is poured with specified solution such as nutrient solution and/or biocide solution；Heat and/or cool plant Plant in object container；Illumination is provided；Shake container for plants；Plant is applied and is dried；Rain and/or snow and/or hail apply；It is high Pressure or low pressure atmospheric environment apply；Sootiness or gas apply；Or sound or sound wave are handled.Other processing are also possible to pollination and make With or hormone apply, or inoculation and/or insect and/or microorganism and/or fungal infection apply.In general, the processing is for sieving It selects in program, wherein such as response of one plant of test or plurality of plants to processing, or the one or more chemical compounds of test Or influence of one or more biological agents to plant or plant growth.

The processing preferably divides the district's groups for the plurality of plants for having carried out same treatment and carries out.Therefore, equipment of the invention mentions Supplied a kind of fully automatic system to carry out randomized block experiment, with test the effect of particular procedure or identify one group of processing it In for expectation or unexpected effect optimal representation processing；Such as the phase jljl using different dilutions or different formulations The foliage applying that matter carries out.The container for plants with processed plant is randomly dispersed in growth position by randomisation step after processing It sets, to minimize the orientation or edge effect of growth position.

In a preferred embodiment, the height of effect that equipment of the invention can be realized foliar treatment, preferably spray Output assessment comprising step as provided.In a more preferred embodiment, which also provides dry system System, wherein container for plants passes through the drying system after sprinkling processing.

In another preferred embodiment of the present, processing is with standard solution (nutrient solution as defined in such as or defined biocidal Agent) irrigating plant container.

The equipment can also include one or more identifier readers, associated with the plant in container for plants to identify Identifier, which preferably provides the output of digital form.The example of this reader is but not limited to bar code reading Device, answering machine reader and RFID reader.In a preferred embodiment, which further includes at least one electronic code Reading device, to identify identifier associated with the plant.The identifier reader is being calculated preferably by Integrated Simulation In equipment and from being wherein fed to database.Database can be manipulated to check and compare the various features for determining plant Data.The equipment preferably includes required amount of identifier reader, so as to determine the plant in equipment at any position The mark of object: before or after being randomized the last stage, in processing stage, after treatment be randomized the stage before and/or it Afterwards.

In another preferred embodiment of the present, which further includes at least one imaging system.Preferably, imaging system includes One or more detectors.It is further preferred that imaging system includes at least one detector, it most preferably include at least one number Word video camera.The term " detector " being such as used in the present invention can refer to any kind of detector, be preferably used for electromagnetism The detector of wave.The term " electromagnetic wave " being such as used in the present invention may include visible light, infrared light and near infrared light.It can be with Including monochromatic light and broad-spectrum light, and it may include incoherent light and coherent light.Other types of electromagnetic wave is also can With.It is highly preferred that detector may include for selected from least one visible, infrared and ultraviolet wavelength region spectral wavelength area The detector of light in domain and most preferably video camera.The video camera, which can be, preferably has space and/or temporal resolution Digital camera.It is highly preferred that video camera is row scanning camera.

In a preferred embodiment, processing system and imaging system can operate independently of one another or they can be with Cooperation, to allow equipment that there is very big versatility: for example, plant is moved to imaging system from growth position to be divided Analysis, processing system can be moved to from growth position to be handled by being then moved back to growth position or plant, be then moved back to Imaging system can be moved to be analyzed from growth position to growth position or plant, be subsequently moved to processing system To be handled (vice versa), growth position is then moved back to.Therefore, which allows one group of plant in imaging systems Object container imaging, and at the same time handling another group of container for plants in the processing system without interfering with each other.

The equipment may also include at least one image analysis apparatus.The image analysis apparatus may be adapted to execute at least one figure The image analysis at least once of picture, it is preferable that the image analysis apparatus may be adapted at least one feature for exporting plant.Based on logical Appropriate software is crossed to the analysis of image to measure one or more of features.If desired, algorithm can be used to assess and survey Fixed one or more features.

The equipment can also have at least one database, be used to record with plant and carry out to each plant primary Or the data repeatedly handled.The data preferably can be at least one of following: at least one image of plant；From plant At least one or more feature derived from least one image of object or character；Information from identifier；Plant is carried out Processing.As being outlined above, at least one described feature or character may include characterize plant feature or one or more of character A parameter.

One or more of features include one or more of the following: the physical behavior of the observable of plant, phenotype Character, metabolic trait, color, green degree, yield, growth, biomass, maturity, transgene traits, bloom, the use of nutrient, The use or disease of water, the influence of pest and/or adverse circumstance.Preferably, one or more of features include area, height, width The presence or absence of degree, leaf angle, the number of sheets, inflorescence and/or one or more of quantity, branch number and branching pattern/branching pattern.

In a preferred embodiment, equipment of the invention can be used for detecting can measuring by being imaged for plant Any feature.Image can be obtained from aerial plant position and/or plant root.Aerial plant position can be branch, leaf, divide One or more of tiller, inflorescence, flower, seed.The system for root imaging is described in WO2010/031780.

In another preferred embodiment of the present, what equipment of the invention can be used for detecting plant can be by obtaining plant Small sample is come any feature for measuring.Sample can be obtained from any position of plant, such as ground position, such as leaf or flower, Or it is obtained from underground position, such as root or root microorganism group.Then for example by carrying out generation to one or more metabolic characteristics It thanks to object edge analysis/metabolism spectrum analysis or the presence or absence of certain micro-organisms is determined by microbiological analysis or is directed to tissue or cellular water Flat morphological analysis analyzes sample.

At least one described feature or character can be preferably selected from: quantitative character, biochemistry/biochemical trait and form One or more of character.In a preferred embodiment, biochemical trait is selected from oil ingredient, protein component, carbon aquation Polymer component, fibre fractionation, oil content, protein content, carbohydrate content, content of starch, fiber content, dry weight and water The group of content composition.In another preferred embodiment, morphological characters be selected from plant framework, plant size, plant shape, Ground biomass, vegetation color, plant growth rate, leaf surface texture, plant weight, plant integrality, leaf integrality, Ye Yan Color, leaf shape, leaf size, leaf growth rate, underground biomass, root growth rate, root thickness, root long degree, root anchoring, inflorescence frame Structure, flower size, flower-shape shape, flower color, flower surface texture, flower weight, flower integrality, endosperm size, plumule size, seed shape Shape, seed size, seed color, the surface of the seed texture, seed weight, seed density and seed integrity.As used herein, Integrality infected to disease, insect/insect pest and the neurological susceptibility of any of fungal infection or resistance it is related.

In an alternative preferred embodiment, it is (such as hairy that quantitative character is selected from the amount of (green) leaf, the amount of root The amount of root and/or branch root), the amount of little Hua, the amount of seed, the amount of deaf-seed, branch amount, seed weight, seed weight and/ Or full rate.However, the combination of other types of parameter and/or the parameter referred to and/or other parameters is possible, such as: Biomass on the ground of each plant and unit area；The underground biomass of unit area；Ground biomass (such as the kind of plant Son or nutrition position) in oil, starch and/or protein content；Flower (little Hua) number of each plant；Or the framework of improvement, Such as the improvement of increased stem diameter, thickness or physical property (such as elasticity).

In another preferred embodiment of the present, one or more of features include one or more different metabolins, and And may need to assess the presence or absence of specific metabolite, the quantity of metabolin, the amount of specific metabolite, etc..

Other than it may handle to plant offer, equipment permission according to the present invention is not artificially being done after the treatment It derives in the case where pre- about plant characteristics or the data of character.It can be used for various purposes, is used especially for assessment and kills livestock Agent, such as the herbicide of foliage applying difference dilution.This in use, the equipment is provided for assessing processed one The integrated automated procedure of the one or more features and/or phenotype of strain or plurality of plants.By using the equipment, people can be with Required data related with the interested key parameter of plant breeder are derived in single operation, such as specific compound is most The optimal formulation of the effective quantity or specific compound of good dilution or compound.Other purposes that the equipment can be used include Form is the assessment of chemical compound or the growth helping matter as microbial suspension.

In addition, the equipment may include control system, which may adapt to control and/or drive imaging system And/or conveyer and/or conveyer belt system and/or image analysis apparatus and/or reader and/or database and/or power supply.It should Control system may include computer and electric connector and/or signal connector, preferably electric wire and interface.

Preferably, imaging system is by shielding natural light.Light inside imaging system can be provided by one group of standardized lamp, The intensity of the lamp can be controlled.

Image Analysis software online processing can be used in the image obtained in imaging system, related with plant to extract Information, and preferably, processed data and image are associated with the unique identifier of corresponding plant, and more Preferably it is downloaded to computer.

In a preferred embodiment, imaging system include the following:

With at least one digital camera of sensitivity in visible, infrared and/or near infrared range；

At least one spectrometer is made of the optical dispersive element of such as grating or prism, many narrow to split the light into Adjacent wavelength band, the spectrometer be placed on before video camera and be it is adjustable, so as to select specific band And it is transmitted to video camera in a predetermined order；

At least one suitable optical lens；

For irradiating at least one light source near infrared range with suitable spectral composition of the plant,

Computer hardware element and from the connector of different aforementioned components, and

Special-purpose software element is used to drive the signal from the hardware element to export and to the hardware element Signal input, and the different step of method described herein is executed automatically.

This imaging is commonly referred to as imaging spectrography in the literature, is in the continuous frequency band of many spectrum while to obtain Take the image that space is registered altogether.The image generated by this imaging spectrography is similar to the figure generated by digital camera Picture, each pixel have many light intensity data bands rather than only except three bands: red, green and blue.In this field In, word " ultraphotic spectrum " data set be described as the number by relatively narrow bandwidth (for example, 1-10nm) it is relatively large (for example, Band composition 100-1000), and " multispectral " data set is usually the less of relatively large bandwidth (for example, 70-400nm) (for example, 5-10) band.

In a preferred embodiment, imaging system includes super spectrum camera.In another preferred embodiment of the present, at As system includes multispectral video camera.

Another aspect provides the effects that equipment as described herein is used to assess the processing to plurality of plants Purposes.Preferably, this equipment uses in method as described herein.

In another aspect, equipment as described herein can be for comparing the effect to the different disposal of similar plants Method in use.

In another aspect, equipment as described herein can compare plant in the effect for the processing about plurality of plants It is used in the method for the influence of the different growth conditions of object.

In another aspect, equipment as described herein can be used for the effect by measuring the processing in such as breeding experiment Fruit screens plant group.

In another aspect, equipment as described herein can make in the method for the effect for test processes plant With, such as one or more of following: the test of specific fertilizer and/or nutrient；The test of specific pesticide；In response to specific The effect of processing and selection and/or cultivation to the plant with one or more required characteristics；Particular procedure, for example using fertilizer Material, nutrient and/or biocide to plant or plant specimen carry out 1,2,3,4,5,6,7,8,9,10,11,12,13,14, 15, the test of the effect and/or validity of 16,17,18,19,20 processing.

At an alternative aspect, equipment described herein can for handle related phenotypic analysis and/ Or for being selected based on the phenotype of processed plant and/or metabolism spectrum scoring in metabolin profile/metabolism spectrum analysis method Select optimal genotype.

In another alternative aspect, equipment as described herein can be for analyzing processed plant sample Resistance method in use.

Conclude thought of the invention, following implementation it is preferable that

Embodiment 1: a method of effect that assessing the processing to plurality of plants for high production, the method includes Following steps:

Multiple container for plants 120 that at least one plant of plant 121 grows in the container for plants are provided

Container moving system 130 for moving the container for plants is provided

Randomization system 140 before the processing of sequence of the offer for being randomized the container for plants

Processing system 150 is provided, and

Randomization system 160 after offer processing,

Wherein, each container for plants 120 is randomized before being moved to the processing by the container moving system 130 System 140,

Randomization system 140 is by the order random-ising of container for plants 121 before the processing,

Then the container 121 is moved to the processing system 150 by the container moving system 130,

The processing system 150 provides at least single treatment,

Hereafter, the container is moved to randomization system 160 after the processing by the container moving system 130,

Randomization system 160 executes second of randomization of the sequence of the container for plants after the processing,

The container 120 is moved to growth position and then assesses the effect of the processing by the container moving system 130 Fruit.

Embodiment 2: according to method described in embodiment 1, wherein the plant in the container for plants and unique Identifier 180 is associated.

Embodiment 3: the method according to any one or more in foregoing embodiments, the method are also provided to A few imaging system 190, wherein the container for plants passes through the imaging system.

Embodiment 4: according to method described in previous embodiment, wherein the container for plants is in the processing Pass through imaging system 190 after preceding and/or processing.

Embodiment 5: the method according to any one or more in embodiment 3 or 4, wherein using it is described at The assessment of the effect of the processing is carried out as system 190.

Embodiment 6: the method according to any one or more in foregoing embodiments, wherein the imaging system System includes one or more detectors 191.

Embodiment 7: according to method described in previous embodiment, wherein the detector includes video camera, preferably Ground digital camera.

Embodiment 8: the method according to any one or more in foregoing embodiments 3 to 7, the imaging system 190 also provide:

Electromagnetic wave is directed on the plant, the electromagnetic wave for emitting from the plant or reflecting is consequently formed；

The plant is imaged at different wavelengths by the detector 191, thus to obtain the image including pixel；

Based on the described image that the pixel alignment records at different wavelengths, 3-D image, the three-dimensional are thus generated Image includes 2 Spatial Dimensions and 1 spectral Dimensions；

Using the conventional mathematical prediction model for the weighted contributions for combining different wave length, thus to obtain the mostly light of the plant Spectrum or Hyper spectral Imaging cube；

Pass through suitable software from the imaging cube bulk measurement one or more features.

Embodiment 9: according to method described in previous embodiment, wherein in the visible, infrared and/or close of spectrum Described image is collected in infra-red range, under many different narrow-bands preferably between 900 nanometers and 1800 nanometers.

Embodiment 10: the method according to any one or more in embodiment 6 to 9, wherein the detector At least one spatial resolution images is obtained, this method, which is also provided, measures one or more spies from described image by suitable software Sign, to provide result information.

Embodiment 11: the method according to any one or more in foregoing embodiments 3 to 10, wherein described The one or more features of the plant are imaged imaging system 190, and the institute of the plant is analyzed by computer disposal The image of one or more features is stated, and result information is related to 180 information of unique identifier for being used for the plant 121 Connection.

Embodiment 12: the method according to any one or more in embodiment 9 to 11, wherein one Or multiple features include one or more of the following: the physical behavior of the observable of plant, phenotypic character, metabolic trait, face Color, yield, growth, biomass, maturity, transgene traits (character changed due to the presence of transgenosis), is opened green degree Flower, the use of nutrient, the use of water or the influence of disease, pest and/or adverse circumstance.

Embodiment 13: according to method described in previous embodiment, wherein one or more of features are quantity One or more of character, biochemical trait and morphological characters.

Embodiment 14: the method according to any one or more in embodiment 8 to 12, wherein one Or multiple features include the presence or absence of area, height, width, Ye Jiao, the number of sheets, inflorescence and/or quantity, branch number and branching pattern/ One or more of pattern.

Embodiment 15: the method according to any one or more in embodiment 8 to 12, wherein the method Further include the steps that the result information of the one or more features of one plant of analysis or plurality of plants to determine the influence of processing.

Embodiment 16: according to method described in embodiment 1, wherein the plurality of plants include one plant or more plants and turn Gene plant.

Embodiment 17: according to method described in embodiment 1, wherein one plant of selection or plurality of plants are further to use In plant breeding or Promoting Experiment or for introducing further modification.

Embodiment 18: according to method described in embodiment 7, wherein described image and/or information are from aerial plant What position and/or plant root obtained.

Embodiment 19: according to the method for claim 18, wherein aerial plant position includes branch, leaf, divides Tiller, inflorescence, flower, seed or any combination thereof.

Embodiment 20: the method according to any one or more in foregoing embodiments, wherein the processing is Foliage spray processing.

Embodiment 21: according to method described in previous embodiment, the method also provides drying system 170, institute The method of stating further includes the steps that wherein container for plants passes through drying system after foliage spray processing.

Embodiment 22: the method according to any one or more in embodiment 1 to 19, wherein the processing It is with standard solution irrigating plant container.

Embodiment 23: according to method described in previous embodiment, wherein the standard solution is defined nutrition Liquid.

Embodiment 24: according to method described in embodiment 22, wherein the standard solution is defined biocide Solution.

Embodiment 25: a kind of equipment for applying the processing to multiple container for plants 120 for high production, at least one plant plant Object 121 is grown in the container for plants,

The equipment includes:

For moving the container moving system 130 of the container for plants；

Randomization system 140 before the processing for the sequence for being used to be randomized the container for plants,

Processing system 150, and

Randomization system 160 after processing,

Wherein, each container for plants 120 is moved to the place into the equipment from the container moving system 130 Randomization system 140 before managing, the sequence of container for plants are to further move to the processing on the container moving system 130 It is randomized before system 150 by randomization system 140 before the processing, the container for plants 120 quilt in the processing system Then processing, the container for plants 120 are moved to randomization system 160 after processing via container moving system 130, after processing with Machine system 160 provides second of randomization of the sequence of the container for plants, and

Hereafter, container for plants 120 is removed from the equipment by the container moving system 130.

Embodiment 26: according to equipment described in previous embodiment, wherein the equipment further includes unique identifier Reader 181.

Embodiment 27: the equipment according to any one or more in embodiment 25 or 26, the equipment are also wrapped Include at least one imaging system 190.

Embodiment 28: according to equipment described in previous embodiment, wherein the imaging system 190 includes one Or multiple detectors 191.

Embodiment 29: according to equipment described in previous embodiment, wherein the imaging system includes at least one Detector, preferably at least a digital camera.

Embodiment 30: the equipment according to any one or more in embodiment 25 to 29, wherein the processing System 150 includes foliage spray system 151.

Embodiment 31: according to equipment described in previous embodiment, wherein the equipment further includes drying system 170。

Embodiment 32: the equipment according to any one or more in embodiment 25 to 29, wherein the processing System 150 includes irrigation system 152.

Embodiment 33: the equipment according to any one or more in embodiment 27 to 32, wherein the imaging System 190 includes:

At least one spectrometer is made of the optical dispersive element of such as grating or prism, many narrow to split the light into Adjacent wavelength band, the spectrometer be placed on video camera before/upstream and be it is adjustable, it is specific so as to select Wave band is simultaneously transmitted to video camera in a predetermined order；

At least one suitable optical lens；

For irradiating at least one light source near infrared range with suitable spectral composition of the plant；

Computer hardware element and from the connector of different aforementioned components；

Special-purpose software element is used to drive the signal from the hardware element to export and to the hardware element Signal input, and the different step of method described in any one of being performed automatically in embodiment 1 to 24 and 39 to 41.

Embodiment 34: the equipment according to any one or more in embodiment 27 to 33 is for assessing to more plants The purposes of the effect of the processing of plant.

Embodiment 35: the equipment according to any one or more in embodiment 27 to 33 is according to embodiment Purposes in method described in any of 1 to 24 and 39 to 41.

Embodiment 36: the equipment according to any one or more in embodiment 27 to 33 is for about to more The effect of the processing of strain plant compares the purposes in the method for the influence of the different growth conditions of plant.

Embodiment 37: the equipment according to any one or more in embodiment 27 to 33 is being used for phenotypic analysis And/or for the table in the assessment of the effect based on the processing to plurality of plants in metabolin profile/metabolism spectrum analysis method Type is metabolized score to select the purposes of optimal genotype.

Embodiment 38: according to any one of embodiment 27 to 30 or the multinomial equipment for testing to plant Purposes in the method for the effect of processing, such as one or more of following: the test of specific fertilizer and/or nutrient；It is special Determine the test of biocide；In response to particular procedure effect and selection to the plant with one or more required characteristics And/or it cultivates；Particular procedure, for example using fertilizer, nutrient and/or biocide to plant or plant specimen carry out 1,2, 3, the test of the effect and/or validity of 4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20 processing.

Embodiment 39: according to method described in embodiment 2, wherein the unique identifier 180 and unique identifier Reader 181 communicates.

Embodiment 40: the method according to any one or more in embodiment 1 to 19, wherein the processing It is to ground plant parts.

Embodiment 41: according to method described in embodiment 22, wherein standard solution includes growth helping matter conduct Chemical compound or microbial suspension.

Embodiment 42: the equipment according to any one or more in embodiment 25 to 29, wherein the processing System 150 includes the sprinkling system 151 for being suitable for spraying ground plant parts.

In order to become more fully apparent the present invention, be next read in conjunction with the figure to apparatus and method according to the invention with And they description in order to demonstrate the invention select according to the method for the present invention in purposes description.These realities Example is applied only by way of explanation, it is not intended to limit or limit the scope of the invention completely in other ways.

Detailed description of the invention

Fig. 1 is an embodiment for high production assessment to the method and apparatus of the effect of the processing of plurality of plants Schematic diagram.

Fig. 2A and 2B is the signal for high production assessment to the method and apparatus of the effect of the sprinkling processing of plurality of plants Figure.

Fig. 3 A and 3B show the effect sprayed and be dried for combined type of the high production assessment to plurality of plants The schematic diagram of method and apparatus.

Fig. 4 shows the signal for high production assessment to the method and apparatus of the effect of the pouring processing of plurality of plants Figure.

110: the equipment for the high production processing to plurality of plants

120: container for plants

121: plant

130: container moving system (not shown)

140: randomization system before handling

141: randomization system before spraying

150: processing system

151: sprinkling system

152: irrigation system

160: randomization system after processing

161: randomization system after sprinkling

170: drying system

180: unique identifier (not shown)

181: unique identifier reader

190: imaging system

191: detector (not shown)

192: image

193: image analysis apparatus

200: control system

Specific embodiment

Embodiment

Figure 1A is the example for high production assessment to the method and apparatus of the effect of the processing of plurality of plants.Plant 121 The growth in the container for plants 120 (in this case, it is greenhouses) in growth place.This greenhouse to container for plants provide by The environment for controlling weather conditions is controllably supplied nutrient and water supply.But it is self-evident, in the container for plants in this greenhouse Variation (microcosmic weather of the plant by growing environment caused by the variation supplied as such as temperature, humidity, light, nutrition and water Variation) it influences, the variation depends on the position (azimuthal effect) of container for plants in the greenhouse.In greenhouse or grow the separate of platform Plant in the container for plants at center is exposed to the plant in the container for plants at the center of the heart or one group of plant in the greenhouse not Same micro (edge effect).In general, container for plants is in a row disposed or is placed on the desk in this greenhouse, and It is handled by another position that container for plants is moved in greenhouse and the phenotype of environmental correclation and/or metabolin ingredient Problem.In the case where placement in a row, most commercial system is worked with first in first out or first-in last-out.This cannot be provided The true randomization of plant or container for plants.System and method through the invention overcome this point.

110 (not shown) of equipment includes 130 (not shown) of container moving system for moving container for plants 120.Plant Container 120 is moved in equipment 110 from growth position.The equipment further include: for being randomized before the processing of container for plants at random Change system 140；Processing system 150；With randomization system 160 after processing.In the method for the invention, each container for plants 120 It is moved in equipment by container moving system 130 and is moved to randomization system 140 before processing.Container for plants is then by the processing Preceding randomization system 140 is randomized, and randomization system 140 uses original culture position as randomization factor before handling.Hereafter, Then container 120 handles container for plants in the enterprising moved further of container moving system 130 to processing system 150, processing system 150 In plant 121.Hereafter, the container for plants 120 for accommodating processed plant is moved to via container moving system 120 executes plant Randomization system 160 after the processing of second of randomization of object container, but processing now is randomization factor.Hereafter, pass through appearance Device mobile system removes container for plants from the device.Preferably, container for plants is then moved to plant growth position, such as Greenhouse or screening room.According to processing, assessment pair after the certain time after leaving equipment of the invention immediately or in container for plants The effect of the processing of plant in container for plants.This assessment can by the plant in plant growth position scored come It visually completes, or can complete in an automatic fashion.This automation may need that growth position will be in above plant Plant imaging, or can be executed by taking container for plants to imaging system, for example, as in wherein plant by being imaged Described in WO 2010/031780.

It, will after being randomized before randomisation step and after processing and processing in the exemplary implementation scheme of Figure 1B Container for plants imaging.Should be imaged for second can carry out immediately after randomization, or only have an opportunity in plant in growth position It is carried out after forming a period of time of (complete) effect of processing.By suitable software from the one or more of image measurement plant Feature.

If desired, algorithm can be used to assess the one or more features of measurement.

Imaging system 190 includes detector 191.In the embodiment of Figure 1B, detector 191 is digital camera/photograph Camera.

The imaging system of Figure 1B can also include at least one 193 (not shown) of image analysis apparatus.Image analysis apparatus 193 may be adapted to the image analysis at least once for executing at least one of image 192, it is preferable that image analysis apparatus 193 can fit In at least one feature or character of the plant for generating imaging.Term " generation " according to the present invention can refer to for example by image Analysis export.

Equipment of the invention can also include 181 (not shown) of identifier reader, to identify and the plant in container for plants The associated identifier of a group plant in object or even container for plants.This reader can be bar code reader, answering machine Reader and/or RFID reader.In a preferred embodiment, which further includes that at least one electronic code reads dress It sets, to identify identifier associated with the plant.

The device can also have at least one database (not shown), be used to record and plant, processing and processing The related data of effect, i.e. plant one or more features after the treatment and/or before the processing of specified plant and it The difference of one or more features afterwards.The data preferably can be at least one of following: on the ground and/or underground At least piece image of plant；At least one feature or character of at least piece image from plant；From what is obtained by plant At least one or more feature that the metabolite analysis of plant sample derives；Information from identifier；The related letter with processing Breath；With the effect time of timing related information really is carried out after processing.As being outlined above, at least one described feature or property Shape may include the one or more parameters for characterizing the phenotype of plant.In a preferred embodiment, method of the invention is available In any feature that can be measured by being imaged of detection plant.Image can be from aerial plant position and/or plant root It obtains.Aerial plant position can be one or more of branch, leaf, tiller, inflorescence, flower, seed.It is preferably implemented at one In scheme, it is characterized in one or more of quantitative character, biochemical trait and morphological characters.In a more preferred In, biochemical trait be selected from by oil ingredient, protein component, carbohydrate ingredient, amino acid composition, fibre fractionation, oil content, Protein content, carbohydrate content, content of starch, amino acid content, secondary metabolites content, fiber content, dry weight and The group of water content composition.In another more preferred embodiment, morphological characters is selected from plant framework, plant size, plant Object shape, branch, ground biomass, vegetation color, plant growth rate, leaf surface texture, plant weight, plant integrality, Leaf integrality, leaf color, leaf shape, leaf size, leaf growth rate, underground biomass, root growth rate, root thickness, root long degree, Root branch, root anchoring, inflorescence framework, flower size, flower-shape shape, flower color, flower surface texture, flower weight, flower integrality, endosperm ruler Very little, embryo size, shape of the seed, seed sizes, seed color, the surface of the seed texture, seed weight, seed density and seed are complete Whole property.As used herein, integrality with disease, insect/insect pest are infected and the neurological susceptibility of any of fungal infection Or resistance is related.In an alternative preferred embodiment, quantitative character be selected from the amount of (green) leaf, the amount of root (such as The amount of hairy and/or branch root), the amount of little Hua, the amount of seed, the amount of deaf-seed, branch amount, seed weight, seed gross weight Amount and/or full rate.However, the combination of other types of parameter and/or the parameter referred to and/or other parameters is possible, Such as: the ground biomass of each plant and unit area；The underground biomass of unit area；Ground biomass (such as plant Seed or nutrition position) in oil, starch and/or protein content；Flower (little Hua) number of each plant；Or the frame of improvement Structure, such as the improvement of increased stem diameter, thickness or physical property (such as elasticity).

In addition, the equipment may include control system 200, the control system 200 may adapt to control and/or drive at As system 190 and/or container moving system 130 and/or image analysis apparatus 193 and/or reader 181 and/or database and/ Or power supply.The control system 200 may include computer and electric connector and/or signal connector, preferably electric wire and interface.

Image Analysis software online processing can be used using the image that imaging system 190 obtains, to extract and plant The related information of one or more features, and preferably, processed data and image is related to unique identifier Connection, and more preferably it is downloaded to computer.

In third exemplary implementation scheme as shown in Figure 2 A, device and method and device and method described in Figure 1A It is identical, but processing is sprinkling processing 151.Sprinkling processing can be based on container for plants one by one in container for plants 120 At least one plant of plant 121 carries out, or sprinkling processing 151 can by the district's groups being made of the plurality of plants in multiple containers come into Row.In the exemplary implementation scheme of Fig. 2 B, district's groups are made of 12 container for plants.But it will be recognized that can be with Any amount of container for plants is taken to be formed for while spray the district's groups of container for plants.Sprinkling can from top to bottom and/or It is carried out in plant side to be processed.As described above, according to processing, after immediately or leaving equipment of the invention in container for plants The effect of the processing to the plant in container for plants is assessed after certain time.This assessment can be by plant growth position The plant set is scored visually to complete, or can be completed in an automatic fashion.This automation may be needed from plant Plant in growth position is imaged for top, or can be executed by taking container for plants to imaging system, for example, such as Described in the WO 2010/031780 that wherein plant is imaged.

Fig. 3 A shows the another exemplary implementation scheme based on embodiment as shown in Figure 2 A, and wherein this method further includes The step of drying the plant in 170 container for plants.Therefore, equipment 110 includes dry channel or similar device.At sprinkling and drying Reason can be carried out based on container for plants one by one at least one plant of plant 121 in container for plants 120 or sprinkling processing 151 can be carried out with partition group.In the exemplary implementation scheme of Fig. 3 B, district's groups are made of 12 container for plants.But technology people Member is it will be recognized that any amount of container for plants can be taken to be formed for while spray the district's groups with dried plant container. As described above, assessment is to plant after the certain time after leaving equipment of the invention immediately or in container for plants according to processing The effect of the processing of plant in container.This assessment can be by scoring come visual to the plant in plant growth position Ground is completed, or can be completed in an automatic fashion.This automation may need to be in the plant of growth position above plant Object imaging, or can be executed by taking container for plants to imaging system, for example, such as the WO that wherein plant is imaged Described in 2010/031780.

It should be understood to the one skilled in the art that the image-forming step as described in Figure 1B can also be increased to the side of Fig. 2A, 2B, 3A, 3B Method.Then container for plants is imaged after being randomized before randomization before step and after processing and processing.It should be second Imaging can carry out immediately after randomization, or only have an opportunity to form (complete) effect of processing in growth position in wherein plant It is carried out after a period of time of fruit.By suitable software from the one or more features of image measurement plant.If desired, can be with The one or more features of measurement are assessed using algorithm.

Fig. 4 shows another exemplary implementation scheme, wherein device and method and device and method phase described in Figure 1A Together, but processing is to pour processing 152.The particular solution that processing will be available to container for plants is poured, and the solution will include Specific nutrition liquid or biocide solution, or influence any other solution or suspension of plant growth.It then can be with one by one This standard solution is supplied at least one container for plants 120 via pouring based on container for plants, or can be with partition group Ground carries out pouring processing 151.It is used for while pouring to be formed it will be recognized that any amount of container for plants can be taken Fill the district's groups of container for plants.As described above, the timing according to processing, after leaving equipment of the invention immediately or in container for plants Between after assessment to the effect of the processing of the plant in container for plants.This assessment can be by the plant in plant growth position Object is scored visually to complete, or can be completed in an automatic fashion.This automation may be needed will above plant Plant imaging in growth position, or can be executed by taking container for plants to imaging system, for example, as wherein planted Described in the WO 2010/031780 that object is imaged.

It should be understood to the one skilled in the art that the method that the image-forming step as described in Figure 1B can also be increased to Fig. 4.

In a preferred embodiment, imaging system 190 include the following:

With at least one digital camera/camera of sensitivity in visible, infrared and/or near infrared range；

At least one suitable optical lens；

Use up the irradiation plant, near infrared range at least one light of suitable spectral composition Source,

This imaging is commonly referred to as imaging spectrography in the literature, is in the continuous frequency band of many spectrum while to obtain Take the image that space is registered altogether.In the art, word " HYPERSPECTRAL IMAGERY cube " is described as by relatively narrow bandwidth (example Such as, 1-10nm) many spectrally continuous bands compositions multichannel image, and " multispectral " image is usually relatively large Less (for example, the 5-10) band of bandwidth (for example, 70-400nm).

The imaging system includes at least detector 191.This detector can be super spectrum camera.In another preferred reality It applies in scheme, imaging system includes multispectral video camera.

It is further comprising the steps of according to the method for the present invention in another exemplary embodiment:

The digital picture of each plant is collected before treatment and/or after processing.It is imaged by using common RGB color Machine collects the piece image of every plant of independent plant.

One or more features are generated using suitable software.

It determines and contrasts from non-plant background the/different pixel for belonging to plant organ.This is handled using standard picture What algorithm (such as intensity threshold) was realized, wherein the pixel value for being different from predetermined background value is considered to belong to plant object.

It determines and contrasts from remaining vegetable organ the/different pixel for belonging to one or more of features.This is to pass through What standard picture Processing Algorithm (such as Morphological Segmentation) was realized, wherein when the geometric attribute of object corresponds to predetermined dimension, it is right As being identified as such as seed or non-seed, colored, leaf.

It is calculated and each of is identified in the picture individually based on the composite attribute for all independent pixels for constituting each object The metric attribute of object.These attributes especially include the amount of the physical size and plant characteristics in two-dimensional space.

In another exemplary implementation scheme, according to the method for the present invention the following steps are included:

The each plant measured by the identification of specific coded system or every group of plant.Ideally, the coding system Subordination is in the type that can electronically read, such as bar code or answering machine label.

Collect the digital picture of multiple individual plants.In the near infrared range of spectrum (namely in 900nm and 1700nm Between) many different narrow-bands under collect many images of identical each individual plants.

HYPERSPECTRAL IMAGERY cube is generated by being directed at the image recorded at different wavelengths, to generate including 2 The 3 d image of Spatial Dimension (x, y) and 1 spectral Dimensions (z).By these images, each picture in two-dimensional space can be generated The optical absorption spectra of element.

Estimate/estimate based on the habit predictability mathematical model for combining the weighted contributions of different wave length at each pixel The amount of corresponding with each pixel dry matter and basic chemical constituent.

It determines and contrasts from remaining vegetable organ the/different pixel for belonging to one or more of features.This is to pass through What standard picture Processing Algorithm (such as Morphological Segmentation) was realized, wherein when the geometric attribute of object corresponds to predetermined dimension, it is right As being identified as such as seed or non-seed, leaf, flower.

It is calculated and each of is identified in spectrum picture based on the composite attribute for all independent pixels for constituting each object The metric attribute of independent object.These attributes include: the physical size in two-dimensional space estimated by dry weight, and are pushed away Fixed chemical constituent.

Claims

1. a kind of method for the effect for assessing the processing to plurality of plants for high production, the described method comprises the following steps:

At least one plant of plant 121 is provided in the multiple container for plants 120 wherein grown；

Container moving system 130 for moving the container for plants is provided；

Randomization system 140 before processing of the offer for the container for plants to be randomized；

Processing system 150 is provided；And

Randomization system 160 after offer processing,

Wherein, each container for plants 120 is moved to before the processing to be randomized by the container moving system 130 is System 140,

The container for plants 121 is randomized by randomization system 140 before the processing,

The processing system 150 provides at least single treatment,

Randomization system 160 executes second of randomization of the container for plants after the processing,

The container 120 is moved to growth position by the container moving system 130, then the effect of assessment processing.

2. according to the method described in claim 1, wherein, the plant and 180 phase of unique identifier in the container for plants Association.

3. the method also includes providing at least one imaging according to any one of preceding claims or the multinomial method System 190, wherein the container for plants passes through the imaging system 190 before the processing and/or after the processing.

4. according to method described in preceding claims 3, wherein one or more of the imaging system 190 to the plant Feature is imaged, and analyzes by computer disposal the image of one or more of features of the plant, and will Result information is associated with for 180 information of unique identifier of the plant 121.

5. according to the method described in claim 4, wherein, one or more of features include one or more of the following: The physical behavior of the observable of plant, metabolic trait, color, green degree, yield, growth, biomass, maturity, turns phenotypic character Gene character is bloomed, the use of nutrient, the use of water or the influence of disease, pest and/or adverse circumstance.

6. according to any one of claim 4 to 5 or the multinomial method, wherein the method also includes one plant of analysis or The step of result information of the one or more features of plurality of plants is to determine the effect handled.

7. according to the method described in claim 1, wherein, the plurality of plants include one plant or more plants of genetically modified plants.

8. according to any one of preceding claims or the multinomial method, wherein the processing is foliage spray processing.

9., the method also includes providing drying system 170, the method is also wrapped according to method described in previous item claim Include the step of making the container for plants after foliage spray processing by drying system.

10. it is a kind of for high production apply at least one plant of plant 121 the multiple container for plants 120 wherein grown processing Equipment,

The equipment includes:

For moving the container moving system 130 of the container for plants；

Randomization system 140 before processing for the container for plants to be randomized；

Processing system 150；With

Randomization system 160 after processing,

Wherein, each container for plants 120 is moved to the processing into the equipment from the container moving system 130 Preceding randomization system 140, the container for plants are to further move to the processing system on the container moving system 130 It is randomized before 150 by randomization system 140 before the processing, the container for plants 120 quilt in the processing system Then processing, the container for plants 120 are moved to randomization system after the processing via the container moving system 130 160, randomization system 160 provides second of randomization of the container for plants after the processing,

Hereafter, the container for plants 120 is moved out of from the equipment by the container moving system 130.

11. according to equipment described in previous item claim, wherein the equipment further includes unique identifier reader 181.

12. any one of 0 or 11 or multinomial equipment according to claim 1, the equipment further includes at least one imaging System 190.

13. any one of 0 to the 12 or multinomial equipment according to claim 1, wherein the processing system 150 includes leaf Face sprinkling system 151.

14. according to equipment described in previous item claim, wherein the equipment further includes drying system 170.

15. any one of 0 to the 14 or multinomial equipment is used to assess the effect of the processing to plurality of plants according to claim 1 The purposes of fruit.

16. any one of 0 to the 14 or multinomial equipment is according to claim 1 to any one of 9 institutes according to claim 1 The purposes in method stated.