CN109055364A - A kind of instrument for extracting nucleic acid and its application method - Google Patents
A kind of instrument for extracting nucleic acid and its application method Download PDFInfo
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Abstract
The invention discloses a kind of instrument for extracting nucleic acid, utilize combination magnetic mechanism, the extraction of sample nucleic acid and the concentration for nucleic acid are realized simultaneously, including feeding mechanism, multiple deep-well plates, magnetic mechanism, magnetic-adsorption part is provided in the magnetic mechanism, the quantity of the magnetic mechanism is multiple and corresponding be distributed on feeding mechanism forms combination magnetic mechanism, the corresponding matching in position of the distributing position and deep-well plates of the magnetic mechanism, various sizes of magnetic-adsorption part is provided in different magnetic mechanisms, hole slot volume matching corresponding with various sizes of magnetic-adsorption part respectively in the deep-well plates.The mutual cooperation that the present invention passes through combination magnetic mechanism and the deep-well plates of multiple and different volume hole slots, the process for extracting, gradient rinsing and eluting can be smoothly sequentially completed, realize the process eluted from bulk sample to micro-scale volume purified product, so that the nucleic acid concentration finally obtained is very high, the extraction effect for nucleic acid is greatly improved.
Description
Technical field
The present invention relates to nucleic acid extraction technical fields, and in particular to a kind of instrument for extracting nucleic acid and its application method.
Background technique
Nucleic acids research is modern biology, the important topic in medical research.Carrier of the nucleic acid as hereditary information be
The material base of gene expression, in addition to normally grow in organism, develop and breed etc. in vital movements have it is particularly significant
Effect outside, the abnormal conditions of it and life, such as tumour generation, radiation insult, genetic disease also have substantial connection.
Instrument for extracting nucleic acid is the instrument that the extraction work of sample nucleic acid is automatically performed using matched nucleic acid extracting reagent
Device, instrument for extracting nucleic acid are divided into two classes: one kind is large-scale automation, commonly referred to as automated fluid work station；Another kind of is small
The automatic instrument for extracting nucleic acid of type is automatically performed extraction purification process using packaged matched reagent.Large-scale automated fluid work station
Because equipment cost is high, operating cost is high, is suitble to once extract thousands of a one species samples, so be really applied
It is fewer.And the instrument of small automatic, it is easy to operate to be widely used because instrument and equipment and operating cost are low.
The model of instrument for extracting nucleic acid on the market, magnetic bar can not change, and can only meet bulk sample processing, and large volume is washed
It is de-；Trace sample processing, micro-scale volume elution.Such as the patent document that number of patent application is 201810058189.8, it discloses
A kind of instrument for extracting nucleic acid there is such problem, when needing to handle bulk sample progress micro-scale volume elution, can not carry out
Pattern switching completes the process eluted from bulk sample to micro-scale volume purified product, leads to the nucleic acid concentration finally obtained
It is lower, requirement of experiment can not be flexibly met.
Summary of the invention
Goal of the invention: in order to overcome instrument for extracting nucleic acid existing in the prior art to be unable to complete from bulk sample to micro
The problem of volume purified product elutes, provides one kind and can be realized bulk sample to micro-scale volume purified product and afford height
The instrument for extracting nucleic acid of concentration nucleic acid.
Technical solution: to achieve the above object, the present invention provides a kind of instrument for extracting nucleic acid, including feeding mechanism, Duo Geshen
Orifice plate, magnetic mechanism, magnetic-adsorption part is provided in the magnetic mechanism, and the quantity of the magnetic mechanism is multiple and corresponding points
Cloth is on feeding mechanism, the corresponding matching in position of the distributing position and deep-well plates of the magnetic mechanism, different magnetic mechanisms
On be provided with various sizes of magnetic-adsorption part, the hole slot volume in the deep-well plates respectively with various sizes of magnetic-adsorption part
Corresponding matching, the feeding mechanism are used to for the deep-well plates of different hole slot volumes being respectively moved at corresponding magnetic mechanism,
Under cooperation by the magnetic-adsorption part of correspondingly-sized, according to the sequence that deep-well plates hole slot volume is descending, by magnetic-adsorption
Nucleic acid on part realizes gradient rinsing and elution, in the way of formation combination magnetic mechanism of multiple magnetic mechanisms, and meanwhile it is real
The now extraction of sample nucleic acid and the concentration for nucleic acid.
Further, the magnetic-adsorption part is bar magnet or magnet ring.
Further, the feeding mechanism is disc rotary feeding mechanism or linear feeding mechanism.
Further, the magnetic mechanism is arranged successively according to the size of magnetic-adsorption part is distributed in feeding mechanism
On, so that deep-well plates more convenient, fast and accurately can be transported to below specified magnetic mechanism by feeding mechanism, be mentioned
High working efficiency.
Further, the quantity of the deep-well plates that deep-well plates one, having more at least more than the quantity of magnetic mechanism
It can be used for placing the recycling of the buffer containing magnetic bead and last discarded magnetic bead.
Further, matched multiple positioning seats corresponding with deep-well plates, the positioning are provided on the feeding mechanism
Seat on be provided with positioning groove, the deep-well plates are entrenched in positioning groove so that deep-well plates have on feeding mechanism it is fabulous
Positional stability.
A kind of method for extracting nucleic acid of instrument for extracting nucleic acid, includes the following steps:
1) deep-well plates are chosen first, the buffer containing magnetic bead are placed in the hole slot of this deep-well plates, at it
In remaining deep-well plates, the sample containing lysate and blood will be injected in the hole slot of that maximum deep-well plates of hole slot size, it will
Eluent is injected in the hole slots of that smallest size of deep-well plates of hole slot, then will be injected separately into the hole slot of remaining deep-well plates pair
The rinsing liquid that should be measured；
2) deep-well plates containing buffer are transported to the maximum magnetic mechanism of magnetic-adsorption part size using feeding mechanism
Lower section corresponding position, using the magnetic mechanism controls, its magnetic-adsorption part is moved downward, and magnetic-adsorption part is extracted into hole slot
Magnetic bead in buffer, then magnetic-adsorption part resets；
3) deep-well plates containing sample are transported under the magnetic mechanism for extracting magnetic bead in step 2 using feeding mechanism
Square corresponding position, using the magnetic mechanism controls, its magnetic-adsorption part is moved downward, and the magnetic bead on magnetic-adsorption part is discharged into
In sample in hole slot, and magnetic-adsorption part resets；
4) after one section of reaction time, the nucleic acid in sample is adsorbed on magnetic bead completely, at this point, utilizing step 3 again
In magnetic mechanism controls its magnetic-adsorption parts move downward, extract the magnetic bead containing nucleic acid, and magnetic-adsorption part resets；
5) using feeding mechanism that maximum deep-well plates of hole slot size in the deep-well plates of rinsing liquid will be housed in step 1
It is transported to the lower section corresponding position for extracting the magnetic mechanism of magnetic bead in step 4, utilizes its magnetic-adsorption of the magnetic mechanism controls
Part moves downward, and is rinsed by the rinsing liquid in hole slot to the nucleic acid on magnetic-adsorption part, after rinsing completion, release
Magnetic bead, magnetic-adsorption part reset；
6) using feeding mechanism by the deep-well plates in step 5 be transported to its magnetic-adsorption part size Corresponding matching that
The lower section corresponding position of magnetic mechanism, its magnetic-adsorption part of magnetic mechanism controls move downward, and extract release and rinse in hole slot
Magnetic bead in liquid, magnetic-adsorption part reset；
7) using the operation of step 5 and step 6 as standard, and so on, with the size of hole slot and magnetic-adsorption part from big
Gradient rinsing is carried out to the nucleic acid on magnetic-adsorption part to small sequence；
8) after the process of step 7, magnetic bead is located on the small magnetic-adsorption part of size second, will be filled using feeding mechanism
There are the deep-well plates of eluent to be transported to the lower section corresponding position of the magnetic mechanism with magnetic bead, utilizes its magnetic of the magnetic mechanism controls
Power adsorption piece moves downward, and by eluting in the eluent in hole slot to magnetic bead, after eluting completion, discharges magnetic bead,
Magnetic-adsorption part resets；
9) using feeding mechanism by the deep-well plates that magnetic bead is equipped in step 8 be transported to magnetic-adsorption part it is smallest size of that
The lower section corresponding position of a magnetic mechanism, its magnetic-adsorption part of the magnetic mechanism controls move downward, and extract the magnetic bead in hole slot,
Complete the extraction for nucleic acid；
10) deep-well plates equipped with buffer are transported to corresponding position below the magnetic mechanism in step 9 using feeding mechanism
It sets, using the magnetic mechanism controls, its magnetic-adsorption part is moved downward, will be in the hole slot for being discharged into the deep-well plates of discarded magnetic bead.
Design principle of the invention are as follows: cooperate feeding mechanism using multiple magnetic mechanisms formation combination magnetic mechanism is configured
On multiple deep-well plates, by the way that the hole slot of different volume size specifications is respectively set in different deep-well plates, and multiple
The magnetic-adsorption part to match with the hole slot of these not co-contents is respectively set in magnetic mechanism, these magnetic-adsorption parts are according to magnetic
Inhale mechanism there are many thickness specification, matching corresponding with the volume specification of hole slot, by multiple deep-well plates successively with multiple magnetic
The switching interaction for inhaling mechanism so that after magnetic bead is extracted, can by hole slot volume from big to small in the way of to having core
The magnetic bead of acid successively carries out gradient rinsing, is finally eluted in the hole slot of minimum volume, while realizing mentioning for sample nucleic acid
It takes and the concentration for nucleic acid, because the volume of the hole slot finally eluted realizes extra small capacity, realizes micro body
The effect of product purified product elution, so that the nucleic acid concentration finally obtained is very high.
What needs to be explained here is that core of the invention technological improvement is exactly to have used gradient rinsing, nucleic acid extraction originally
Instrument if large capacity sample extraction nucleic acid, needs to be equipped with large-sized magnetic-adsorption part, when elution, needs corresponding great Rong
The eluent of amount, is not so washed to get off, and the present invention, which passes through, successively shifts magnetic bead using magnetic-adsorption part from big to small, is successively floated
It washes, last magnetic bead concentrates on the smallest magnetic-adsorption part, so it is small to correspond to elution finally only to need the eluent of low capacity
Magnetic bead on bar magnet, in whole process, the amount of magnetic bead is basically unchanged, and the nucleic acid amount combined on magnetic bead is also basically unchanged, and is eluted
The capacity of liquid is greatly reduced, therefore the nucleic acid concentration finally obtained has and greatly improves.
The utility model has the advantages that compared with prior art, the present invention by the magnetic mechanisms of multiple and different magnetic-adsorption part specifications and
The mutual cooperation of the deep-well plates of multiple and different volume hole slots, can smoothly be sequentially completed extract, gradient rinsing and elution
Process realizes the process eluted from bulk sample to micro-scale volume purified product, so that the nucleic acid concentration finally obtained is non-
Chang Gao greatly improves the extraction effect for nucleic acid.
Detailed description of the invention
Fig. 1 is schematic perspective view of the invention；
Fig. 2 is overlooking structure diagram of the invention.
Specific embodiment
In the following with reference to the drawings and specific embodiments, the present invention is furture elucidated.
The feeding mechanism of the present embodiment amplifying nucleic acid extraction apparatus is disc rotary mechanism, and disc rotary mechanism has turntable 100,
Magnetic-adsorption part in magnetic mechanism is selected as bar magnet.
As depicted in figs. 1 and 2, instrument for extracting nucleic acid includes disc rotary mechanism, 6 deep-well plates and 5 magnetic mechanisms, disk
It is recessed that the positioning that 6 positioning seats, 102,6 deep-well plates are entrenched in 6 positioning seats 102 respectively is provided on the turntable 100 of rotating mechanism
In slot, 6 deep-well plates are respectively the first deep-well plates 101, the second deep-well plates 6, third deep-well plates 7, the 8, the 5th depth of the 4th deep-well plates
Orifice plate 9 and the 6th deep-well plates 10,5 magnetic mechanisms are respectively the first magnetic mechanism 1, the second magnetic mechanism 2, third magnetic mechanism
3, the 4th magnetic mechanism 4 and the 5th magnetic mechanism 5,5 magnetic mechanisms are separately positioned on the periphery of turntable 100, wherein the first magnetic
It inhales mechanism 1, the second magnetic mechanism 2, third magnetic mechanism 3, the 4th magnetic mechanism 4 and the 5th magnetic mechanism 5 and is located at second
The surface of deep-well plates 6, third deep-well plates 7, the 4th deep-well plates 8, the 5th deep-well plates 9 and the 6th deep-well plates 10.
First deep-well plates 101, the second deep-well plates 6, third deep-well plates 7, the 4th deep-well plates 8, the 5th deep-well plates 9 and the 6th are deep
24 hole slots are respectively arranged on orifice plate 10 and according to 6 × 4 arrangements, the first deep-well plates 101, the second deep-well plates 6, third deep hole
In plate 7, the 4th deep-well plates 8, the 5th deep-well plates 9 and the 6th deep-well plates 10 the single hole volume of hole slot be respectively 48ml, 50ml,
20ml,10ml,3ml,0.5ml；First magnetic mechanism 1, the second magnetic mechanism 2, third magnetic mechanism 3,4 and of the 4th magnetic mechanism
24 bar magnets and 24 stirring sets are provided in 5th magnetic mechanism 5 and according to 6 × 4 arrangements, the first magnetic mechanism 1, second
Magnetic mechanism 2, third magnetic mechanism 3, the 4th magnetic mechanism 4 and bar magnet in the 5th magnetic mechanism 5 and stirring set are respectively the
One bar magnet 11 and first stirring set the 12, second bar magnet 21 and the second stirring set 22, third bar magnet 31 and third stirring set the 32, the 4th
Bar magnet 41 and the 4th stirring set 42, the 5th bar magnet 51 and the 5th stirring set 52, the first bar magnet 11, the second bar magnet 21, third bar magnet
31, the 4th bar magnet 41 and the 5th bar magnet 51 be its thickness of cylindrical structure according to diameter x length be respectively 11x133mm,
9.6x76mm、4.6x70.5mm、2.1x61mm、1.4x25mm。
It is configured according to above structure, the sample containing lysate and blood is carried out using the instrument for extracting nucleic acid of the present embodiment
Nucleic acid extraction, specific operating process are as follows:
1) buffer containing magnetic bead is placed in the hole slot of the first deep-well plates 101 first, by the hole of the second deep-well plates 6
The sample containing lysate and blood is injected in slot, will inject eluent in the hole slot of the 6th deep-well plates 10, then by third deep hole
Plate 7, the 4th deep-well plates 8, the 5th deep-well plates 9 hole slot in be injected separately into the rinsing liquid of corresponding amount；
2) turntable 100 is rotated using disc rotary mechanism controls, by the first deep-well plates 101 rotation to the first magnetic mechanism 1
Lower section corresponding position, control its first bar magnet 11 using the first magnetic mechanism 1 and move downward, the first bar magnet 11 is stirred by first
It mixes set 12 and extend into magnetic bead in the hole slot of the first deep-well plates 101 in Extraction buffer, then the first bar magnet 11 resets；
3) turntable 100 is rotated using disc rotary mechanism controls, by the second deep-well plates 6 rotation to the first magnetic mechanism 1
Lower section corresponding position controls its first bar magnet 11 using the first magnetic mechanism 1 and moves downward, and it is deep that the first bar magnet 11 extend into second
The magnetic bead on first bar magnet 11 is discharged into sample in the hole slot of orifice plate 6, and the first bar magnet 11 resets；
4) after one section of reaction time, the nucleic acid in sample is adsorbed on magnetic bead completely, at this point, utilizing the first magnetic again
It inhales its first bar magnet 11 of control of mechanism 1 to move downward, extracts the magnetic bead containing nucleic acid, and the first bar magnet 11 resets；
5) turntable 100 is rotated using disc rotary mechanism controls, by the rotation of third deep-well plates 7 to the first magnetic mechanism 1
Lower section corresponding position controls its first bar magnet 11 using the first magnetic mechanism 1 and moves downward, and the first bar magnet 11 extend into third depth
In rinsing liquid in 7 hole slot of orifice plate, the nucleic acid on the first bar magnet 11 is rinsed, after rinsing completion for a period of time, is released
Magnetic bead is put, 11 bar magnet of the first bar magnet resets；
6) turntable 100 is rotated using disc rotary mechanism controls, by the rotation of third deep-well plates 7 to the second magnetic mechanism 2
Lower section corresponding position, the second magnetic mechanism 2 control its second bar magnet 21 and move downward, and the second bar magnet 21 passes through the second stirring set 22
It extend into the hole slot of third deep-well plates 7 and extracts magnetic bead of the release in rinsing liquid, the second bar magnet 21 resets；
7) turntable 100 is rotated using disc rotary mechanism controls, by the 4th deep-well plates 8 rotation to the second magnetic mechanism 2
Lower section corresponding position, the second magnetic mechanism 2 control its second bar magnet 21 and move downward, and the second bar magnet 21 extend into the 4th deep-well plates
In rinsing liquid in 8 hole slot, the nucleic acid on the second bar magnet 21 is rinsed, after rinsing completion for a period of time, release
Magnetic bead, 21 bar magnet of the second bar magnet reset；
8) turntable 100 is rotated using disc rotary mechanism controls, by the 4th deep-well plates 8 rotation to third magnetic mechanism 3
Lower section corresponding position, third magnetic mechanism 3 control its third bar magnet 31 and move downward, and third bar magnet 31 passes through third stirring set 32
It extend into the hole slot of the 4th deep-well plates 8 and extracts magnetic bead of the release in rinsing liquid, third bar magnet 31 resets；
9) turntable 100 is rotated using disc rotary mechanism controls, by the 5th deep-well plates 9 rotation to third magnetic mechanism 3
Lower section corresponding position, third magnetic mechanism 3 control its third bar magnet 31 and move downward, and third bar magnet 31 extend into the 5th deep-well plates
In rinsing liquid in 9 hole slot, the nucleic acid on third bar magnet 31 is rinsed, after rinsing completion for a period of time, release
Magnetic bead, 31 bar magnet of third bar magnet reset；
10) turntable 100 is rotated using disc rotary mechanism controls, by the 5th deep-well plates 9 rotation to the 4th magnetic mechanism 4
Lower section corresponding position, the 4th magnetic mechanism 4 control its 4th bar magnet 41 and move downward, and the 4th bar magnet 41 passes through the 4th stirring set 42
It extend into the hole slot of the 5th deep-well plates 9 and extracts magnetic bead of the release in rinsing liquid, the 4th bar magnet 41 resets；
11) turntable 100 is rotated using disc rotary mechanism controls, by the 6th deep-well plates 10 rotation equipped with eluent to the
The lower section corresponding position of four magnetic mechanisms 4 controls its 4th bar magnet 41 using the 4th magnetic mechanism 4 and moves downward, the 4th bar magnet
Magnetic bead is eluted in 41 eluents extending into 10 hole slot of the 6th deep-well plates, after eluting completion for a period of time, is released
Magnetic bead is put, the 4th bar magnet 41 resets；
12) turntable 100 is rotated using disc rotary mechanism controls, by the 6th deep-well plates 10 rotation to the 5th magnetic mechanism 5
Lower section corresponding position, the 5th magnetic mechanism 5 control its 5th bar magnet 51 move downward, extract 10 hole slot of the 6th deep-well plates in
Magnetic bead completes the extraction for nucleic acid；
13) after to nucleic acid extraction, turntable 100 is rotated using disc rotary mechanism controls, by the equipped with buffer
One deep-well plates 101 turn to the lower section corresponding position of the 5th magnetic mechanism 5, control its 5th bar magnet using the 5th magnetic mechanism 5
51 move downward, and by being discharged into the hole slot of the first deep-well plates 101 for discarded magnetic bead, so far whole flow process terminates.
In above-mentioned experimental procedure, step 1 and 2 complete the magnetic bead for adsorbing nucleic acid extraction, step 3~step 10 is complete
It is rinsed at the gradient descending for the hole slot volume of nucleic acid, step 11 and step 12 complete the smallest by 6th in volume
Nucleic acid is eluted in 10 hole slot of deep-well plates, step 13 completes the recycling for discarding magnetic bead.
It can be seen that 1~step 13 of above-mentioned steps completes the mistake eluted from bulk sample to micro-scale volume purified product
Journey, since the volume of final nucleic acid elution has obtained micro-scale volume, so the nucleic acid concentration finally obtained is very high.
Using same batch of sample, the nucleic acid and existing nucleic acid that are obtained respectively by the experimental procedure of the above instrument for extracting nucleic acid
Extraction apparatus carries out nucleic acid extraction twice, carries out measurement of concetration to obtained nucleic acid, obtains following data contrast table:
| Instrument | Nucleic acid concentration |
| Instrument for extracting nucleic acid 1 of the present invention | 1013ng/ml |
| Instrument for extracting nucleic acid 2 of the present invention | 987ng/ml |
| Existing instrument for extracting nucleic acid 1 | 436ng/ml |
| Existing instrument for extracting nucleic acid 2 | 410ng/ml |
According to upper table it is found that be apparently higher than existing core using the nucleic acid concentration twice that instrument for extracting nucleic acid of the invention obtains
The nucleic acid concentration that sour extraction apparatus obtains.
Claims (9)
1. a kind of instrument for extracting nucleic acid, including feeding mechanism, multiple deep-well plates, magnetic mechanism, magnetic is provided in the magnetic mechanism
Power adsorption piece, it is characterised in that: the quantity of the magnetic mechanism is multiple and corresponding be distributed on feeding mechanism forms combination magnetic
Mechanism is inhaled, the corresponding matching in position of the distributing position and deep-well plates of the magnetic mechanism is provided in different magnetic mechanisms
Various sizes of magnetic-adsorption part, hole slot volume in the deep-well plates respectively with various sizes of magnetic-adsorption part corresponding
Match, the feeding mechanism passes through correspondence for the deep-well plates of different hole slot volumes to be respectively moved at corresponding magnetic mechanism
Under the cooperation of the magnetic-adsorption part of size, according to the sequence that deep-well plates hole slot volume is descending, by the core on magnetic-adsorption part
Acid carries out gradient rinsing and elution.
2. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the magnetic-adsorption part is bar magnet or magnetic
Ring.
3. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the quantity of the deep-well plates is than magnetic mechanism
Quantity it is one at least more.
4. a kind of instrument for extracting nucleic acid according to claim 3, it is characterised in that: the quantity of the deep-well plates is 6, described
The quantity of magnetic mechanism is 5.
5. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the feeding mechanism is disc rotary feeding
Mechanism or linear feeding mechanism.
6. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: be provided on the feeding mechanism and deep hole
Plate corresponding matched multiple positioning seats are provided with positioning groove on the positioning seat, and the deep-well plates are entrenched in positioning groove
It is interior.
7. a kind of instrument for extracting nucleic acid according to claim 1, it is characterised in that: the magnetic mechanism is according to magnetic-adsorption part
Size be arranged successively and be distributed on feeding mechanism.
8. a kind of method for extracting nucleic acid of instrument for extracting nucleic acid according to claim 1, it is characterised in that: including walking as follows
It is rapid:
1) deep-well plates are chosen first, the buffer containing magnetic bead are placed in the hole slot of this deep-well plates, at remaining
In deep-well plates, the sample containing lysate and blood will be injected in the hole slot of that maximum deep-well plates of hole slot size, by hole slot
Eluent is injected in the hole slot of that smallest size of deep-well plates, then will be injected separately into corresponding amount in the hole slot of remaining deep-well plates
Rinsing liquid；
2) deep-well plates containing buffer are transported under the maximum magnetic mechanism of magnetic-adsorption part size using feeding mechanism
Square corresponding position, using the magnetic mechanism controls, its magnetic-adsorption part is moved downward, and magnetic-adsorption part extracts buffering into hole slot
Magnetic bead in liquid, then magnetic-adsorption part resets；
3) lower section for the deep-well plates containing sample being transported to the magnetic mechanism that magnetic bead is extracted in step 2 using feeding mechanism is right
Position is answered, its magnetic-adsorption part moves downward using the magnetic mechanism controls, and the magnetic bead on magnetic-adsorption part is discharged into hole slot
In interior sample, and magnetic-adsorption part resets；
4) after one section of reaction time, the nucleic acid in sample is adsorbed on magnetic bead completely, at this point, again using in step 3
Its magnetic-adsorption part of magnetic mechanism controls moves downward, and extracts the magnetic bead containing nucleic acid, and magnetic-adsorption part resets；
5) using feeding mechanism that maximum deep-well plates of hole slot size in the deep-well plates of rinsing liquid will be housed in step 1 to transport
To the lower section corresponding position for the magnetic mechanism for extracting magnetic bead in step 4, using its magnetic-adsorption part of the magnetic mechanism controls to
Lower movement rinses the nucleic acid on magnetic-adsorption part by the rinsing liquid in hole slot, after rinsing completion, discharges magnetic
Pearl, magnetic-adsorption part reset；
6) deep-well plates in step 5 are transported to that magnetic with its magnetic-adsorption part size Corresponding matching using feeding mechanism
The lower section corresponding position of mechanism, its magnetic-adsorption part of magnetic mechanism controls move downward, and extract and discharge in hole slot in rinsing liquid
Magnetic bead, magnetic-adsorption part reset；
7) using the operation of step 5 and step 6 as standard, and so on, from big to small with the size of hole slot and magnetic-adsorption part
Sequence on magnetic-adsorption part nucleic acid carry out gradient rinsing；
8) after the process of step 7, magnetic bead is located on the small magnetic-adsorption part of size second, will be equipped with and be washed using feeding mechanism
The deep-well plates of de- liquid are transported to the lower section corresponding position of the magnetic mechanism with magnetic bead, are inhaled using its magnetic force of magnetic mechanism controls
Attachment moves downward, and by eluting in the eluent in hole slot to magnetic bead, after eluting completion, discharges magnetic bead, magnetic force
Adsorption piece resets；
9) deep-well plates that magnetic bead is equipped in step 8 are transported to that smallest size of magnetic of magnetic-adsorption part using feeding mechanism
The lower section corresponding position of mechanism is inhaled, its magnetic-adsorption part of the magnetic mechanism controls moves downward, and extracts the magnetic bead in hole slot, completes
Extraction for nucleic acid.
9. a kind of method for extracting nucleic acid of instrument for extracting nucleic acid according to claim 8, it is characterised in that: further include walking as follows
Rapid 10, specifically: the deep-well plates equipped with buffer are transported to below the magnetic mechanism in step 9 using feeding mechanism and are corresponded to
Position, using the magnetic mechanism controls, its magnetic-adsorption part is moved downward, by the hole slot for being discharged into the deep-well plates of discarded magnetic bead
In.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110333311A (en) * | 2019-07-08 | 2019-10-15 | 天津迪沃特生物电子科技有限公司 | A kind of auto extractive and purification device |
| CN110327659A (en) * | 2019-07-19 | 2019-10-15 | 天津迪沃特生物电子科技有限公司 | A kind of extraction and purification method and device based on magnetic fiber material |
| CN111996113A (en) * | 2020-08-18 | 2020-11-27 | 杭州博日科技股份有限公司 | Magnetic bead method nucleic acid extraction device and nucleic acid extraction method thereof |
| CN113046348A (en) * | 2021-05-13 | 2021-06-29 | 安图实验仪器(郑州)有限公司 | Magnetic bead adsorption system and magnetic bead adsorption mechanism thereof |
| CN113846009A (en) * | 2021-08-19 | 2021-12-28 | 韦玉军 | Liquid separating extractor by magnetic bead method |
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| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
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| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN107974394A (en) * | 2018-01-22 | 2018-05-01 | 无锡百泰克生物技术有限公司 | A kind of instrument for extracting nucleic acid |
| CN208995507U (en) * | 2018-09-27 | 2019-06-18 | 无锡百泰克生物技术有限公司 | A kind of instrument for extracting nucleic acid |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110333311A (en) * | 2019-07-08 | 2019-10-15 | 天津迪沃特生物电子科技有限公司 | A kind of auto extractive and purification device |
| CN110333311B (en) * | 2019-07-08 | 2022-05-20 | 苏州艾捷博雅生物电子科技有限公司 | Automatic extraction and purification device |
| CN110327659A (en) * | 2019-07-19 | 2019-10-15 | 天津迪沃特生物电子科技有限公司 | A kind of extraction and purification method and device based on magnetic fiber material |
| CN111996113A (en) * | 2020-08-18 | 2020-11-27 | 杭州博日科技股份有限公司 | Magnetic bead method nucleic acid extraction device and nucleic acid extraction method thereof |
| CN111996113B (en) * | 2020-08-18 | 2021-06-29 | 杭州博日科技股份有限公司 | Magnetic bead method nucleic acid extraction device and nucleic acid extraction method thereof |
| CN113046348A (en) * | 2021-05-13 | 2021-06-29 | 安图实验仪器(郑州)有限公司 | Magnetic bead adsorption system and magnetic bead adsorption mechanism thereof |
| CN113846009A (en) * | 2021-08-19 | 2021-12-28 | 韦玉军 | Liquid separating extractor by magnetic bead method |
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