CN109045368A - A kind of medical high-strength degree high tenacity absorbable composite biomaterial - Google Patents
A kind of medical high-strength degree high tenacity absorbable composite biomaterial Download PDFInfo
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- CN109045368A CN109045368A CN201810726129.9A CN201810726129A CN109045368A CN 109045368 A CN109045368 A CN 109045368A CN 201810726129 A CN201810726129 A CN 201810726129A CN 109045368 A CN109045368 A CN 109045368A
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- composite biomaterial
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- 239000012620 biological material Substances 0.000 title claims abstract description 25
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 238000000465 moulding Methods 0.000 claims abstract description 18
- 229920001661 Chitosan Polymers 0.000 claims abstract description 16
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229920000747 poly(lactic acid) Polymers 0.000 claims abstract description 13
- 239000004626 polylactic acid Substances 0.000 claims abstract description 13
- 239000000126 substance Substances 0.000 claims abstract description 5
- 238000012545 processing Methods 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims 2
- 239000002042 Silver nanowire Substances 0.000 claims 1
- 229910000391 tricalcium phosphate Inorganic materials 0.000 abstract description 10
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 9
- 239000002994 raw material Substances 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 4
- 230000000399 orthopedic effect Effects 0.000 abstract description 3
- 238000005056 compaction Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 11
- 210000000988 bone and bone Anatomy 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 6
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 6
- 229910019142 PO4 Inorganic materials 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 238000002513 implantation Methods 0.000 description 4
- 239000007769 metal material Substances 0.000 description 4
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000001506 calcium phosphate Substances 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 229940078499 tricalcium phosphate Drugs 0.000 description 3
- 235000019731 tricalcium phosphate Nutrition 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 229940058573 b-d glucose Drugs 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 239000011173 biocomposite Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N glucosamine group Chemical group OC1[C@H](N)[C@@H](O)[C@H](O)[C@H](O1)CO MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 229920001523 phosphate polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000005837 radical ions Chemical class 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/022—Metals or alloys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/028—Other inorganic materials not covered by A61L31/022 - A61L31/026
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/042—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/148—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- Epidemiology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention provides a kind of medical high-strength degree high tenacity absorbable composite biomaterial, chemical component contains by mass percentage: nanometer β-Ca3(PO4)210%~15%, chitosan 6%~12%, nano-silver thread 2.5%~4.0%, surplus are polylactic acid；Preparation process of the present invention are as follows: through heat-die compaction at the processing temperature of hot moulding is 75~250 DEG C, and the pressure of hot moulding is 2~10MPa after mixing raw material；Composite biological material prepared by the present invention has the characteristics that intensity height, good toughness, antibacterial, good biocompatibility, has self-bone grafting and osteoacusis active, can be made into various orthopedic fixer tools.
Description
Technical field
The invention belongs to medical new material technology field, in particular to a kind of medical high-strength degree high tenacity can absorb compound life
Object material.
Background technique
Bone fixation means are the implantation intracorporal medical devices of people, are the treatment indispensable medical instrument of orthopaedic disease, mesh
There are three types of preceding common internal fixation device part implantation materials: metal material, inorganic non-metallic and molecular absorbable material.Gold
Belong to material there is needing to carry out higher second operation taking-up, elasticity modulus, stress-shielding effect, internal foreign matter, nothing
Machine is nonmetallic and molecular absorbable material becomes current internal fixation device part widely used biomaterial in implantation material.
Tricalcium phosphate [Ca3(PO4)2] there is chemical composition similar with inorganic phase in bone, it is that most common biology is compound
The filler of material, Chinese patent (CN1403167) and United States Patent (USP) (US5981619) once disclose tricalcium phosphate polymer are added
In obtain the preferable Biocomposite material of mechanical property.Tricalcium phosphate can be special in implantation interfacial initiation as bone renovating material
Biologically, chemical bonding can be formed between material and osseous tissue interface, induce the reparation and regeneration of bone.
Polylactic acid (PLLA) is a kind of novel biodegradable material, have good mechanical property, biocompatibility,
It is degradable and be absorbed, but polylactic acid inactive, and its acid degradation products can cause internal inflammatory reaction, cause office
The damage of portion's tissue.
Chitosan (CS) is that the chitin (chitin) being widely present by nature is obtained by deacetylation, chemistry
Entitled Chitosan (1-4) -2- amino-B-D glucose, biological functionality and compatibility, the blood of this natural polymer
The excellent performances such as liquid phase capacitive, safety, microbic resolvability are by pharmaceuticals industry extensive concern；Chitosan is a kind of degradable
Natural biological polysaccharide has the effects that promote blood coagulation and wound healing；Chitosan and human-body biological compatibility are good, but lack bone
Conduction and bone-inducting active；But chitosan exclusive use mechanical property is undesirable, especially in vivo under wet condition due to inhaling
Aqueous to cause very much mechanical strength loss by force quickly, catabolite is Glucosamine, is in alkalescent.
But no matter which kind of inorganic non-metallic material or molecular absorbable material all exist tenacity it is low, can not be with metal
The deficiency that material is mentioned in the same breath.
Domestic number of patent application is 201210255385.7 to disclose a kind of absorbable composite biomaterial and its preparation side
Method, application No. is 201210524447.X to disclose a kind of absorbable bone screw and its preparation method and application, by both skills
The bone anchor of art preparation deposits intensity in use and the low problem of toughness, and part thereof does not fully recover in patient
When occur as soon as the accidents such as fracture, abrasion.
Summary of the invention
To solve the above-mentioned problems, the invention reside in by nanometer-Ca3(PO4)2, polylactic acid, chitosan, nano-silver thread phase
In conjunction with, a kind of medical high-strength degree high tenacity absorbable composite biomaterial is provided, have intensity height, good toughness, antibacterial, biofacies
Capacitive is good, have the characteristics that self-bone grafting and osteoacusis is active.
Based on object above, the present invention provides a kind of medical high-strength degree high tenacity absorbable composite biomaterials, special
Sign is, described its chemical component of medical high-strength degree high tenacity absorbable composite biomaterial is by mass percentage are as follows: receives
Rice β-Ca3(PO4)210%~15%, chitosan 6%~12%, nano-silver thread 2.5%~4.0%, surplus are polylactic acid.
Nanometer β-the Ca3(PO4)2Partial size be 40~100nm, be in nano-scale particle.
The partial size of the chitosan is 80~320 μm.
The polylactic acid is average molecular weight 1 × 105~7 × 105L-lactic acid.
The diameter of the nano-silver thread is 50~100nm.
Further, a kind of medical high-strength degree high tenacity absorbable composite biomaterial is made by following steps:
By the nanometer β-Ca of formula ratio3(PO4)2, chitosan, nano-silver thread, polylactic acid mixed, then through hot moulding
It is made；The processing temperature of hot moulding is 75~250 DEG C, and the pressure of hot moulding is 2~10MPa.
Innovative characteristics of the invention are as follows: metal material, inorganic non-metallic material, organic material are combined, prepare one
Kind medical high-strength degree high tenacity absorbable composite biomaterial has intensity height, good toughness, antibacterial, good biocompatibility, has
The features such as self-bone grafting and active osteoacusis, can be made into various orthopedic fixer tools.
Nano-silver thread in the present invention not only has antibacterial action, also largely enhances the intensity of material and tough
Property；Nano beta-Ca3(PO4)2With good bioactivity and degradability, a large amount of phosphoric acid for being released in degradation process
The deposition of osteoid apatite mineral can be formed in situ in radical ion and calcium ion, is conducive in internal orthopedic fixer tool degradation process
The regeneration of bone tissue；The porous structure formed after chitosan fast degradation is conducive to growing into for bone tissue, the weak base of degradation product
Property can neutralize the acidity of polylactic acid degradation product.
Detailed description of the invention
Fig. 1 is medical high-strength degree high tenacity absorbable composite biomaterial SEM prepared by the embodiment of the present invention 3.
Specific embodiment
Principles and features of the present invention are described with reference to embodiments, listed give an actual example is served only for explaining this hair
It is bright, not limit the scope of the invention.
Embodiment 1:
1) ingredient: by mass percentage are as follows: the nanometer β-Ca of partial size 40nm3(PO4)210%, 150 μm of partial size of shell is poly-
The nano-silver thread 3.0% of sugar 12%, diameter 60nm, surplus are molecular weight 5 × 105L-lactic acid；
2) raw material prepared being placed in mold, is heated to 150 DEG C of progress hot mouldings, the pressure of hot moulding is 5MPa,
Pressure maintaining 30min；
3) Mechanics Performance Testing；
4) 50mm × 50mm × 10mm test piece antibacterial experiment: is made through anhydrous second in the composite biological material prepared
Alcohol, deionized water ultrasonic cleaning, each 8min are then placed in 75% alcoholic solution of volume fraction and sterilize 45min.Testing bacterium is
Staphylococcus aureus.The test plate (panel) after disinfection is put into culture dish first, addition 50mL bacteria suspension, static gas wave refrigerator 2h, so that
Bacterium is sufficiently sticked；The bacterium that surface porosity is sticked is rinsed out with PBS solution later, is rinsed 3 times altogether；Answering after will be flushed
It closes biomaterial test plate (panel) to put into 50mLPBS solution, sonic oscillation 3min rinses out the bacterium of surface adhesion.It will contain later
There is the PBS solution of surface adhesion bacterium to take 5mL, be uniformly layered on plate, plate is put into 37 DEG C of constant temperature incubation
12h is cultivated in case, observation calculates clump count.
5) bone-inducting active is tested: 100mm × 100mm × 5mm test piece is made in the composite biological material prepared,
Neonate rat Calvarial osteoblast is inoculated in test piece, inoculum density is 300/mm2, with the new life for being 10% containing volume fraction
The DMEM culture medium culture of cow's serum, every 1d change liquid, cultivate 3d and 5d respectively, and then MTT 0.1mL, 37 DEG C of cultures are added in every hole
4h inhales and abandons supernatant, then DMSO 0.5mL is added in every hole, and absorbance is measured at wavelength 490nm with microplate reader.
Embodiment 2:
1) ingredient: by mass percentage are as follows: the nanometer β-Ca of partial size 80nm3(PO4)212%, 300 μm of partial size of shell is poly-
The nano-silver thread 4.0% of sugar 9%, diameter 100nm, surplus are molecular weight 6 × 105L-lactic acid；
2) raw material prepared being placed in mold, is heated to 190 DEG C of progress hot mouldings, the pressure of hot moulding is 8MPa,
Pressure maintaining 30min；
3) Mechanics Performance Testing；
4) it antibacterial experiment: is carried out by embodiment 1；
5) bone-inducting active is tested: being carried out by embodiment 1.
Embodiment 3:
1) ingredient: by mass percentage are as follows: the nanometer β-Ca of partial size 100nm3(PO4)215%, 200 μm of partial size of shell
The nano-silver thread 3.5% of glycan 10%, diameter 75nm, surplus are molecular weight 5 × 105L-lactic acid；
2) raw material prepared being placed in mold, is heated to 205 DEG C of progress hot mouldings, the pressure of hot moulding is 10MPa,
Pressure maintaining 30min；
3) Mechanics Performance Testing；
4) it antibacterial experiment: is carried out by embodiment 1；
5) bone-inducting active is tested: being carried out by embodiment 1.
Comparative example 1:
1) ingredient: by mass percentage are as follows: the nanometer β-Ca of partial size 100nm3(PO4)215%, 200 μm of partial size of shell
Glycan 10%, surplus are molecular weight 5 × 105L-lactic acid；
2) raw material prepared being placed in mold, is heated to 205 DEG C of progress hot mouldings, the pressure of hot moulding is 10MPa,
Pressure maintaining 30min；
3) Mechanics Performance Testing；
4) it antibacterial experiment: is carried out by embodiment 1；
5) bone-inducting active is tested: being carried out by embodiment 1.
Comparative example 2:
1) ingredient: by mass percentage are as follows: the nano-silver thread of the chitosan 10%, diameter 75nm that 200 μm of partial size
3.5%, surplus is molecular weight 5 × 105L-lactic acid；
2) raw material prepared being placed in mold, is heated to 205 DEG C of progress hot mouldings, the pressure of hot moulding is 10MPa,
Pressure maintaining 30min；
3) Mechanics Performance Testing；
4) it antibacterial experiment: is carried out by embodiment 1；
5) bone-inducting active is tested: being carried out by embodiment 1.
Embodiment and comparative example obtains that the results are shown in Table 1.
Table 1
Examples detailed above shows: composite biological material intensity height prepared by the present invention, has antibiotic property and self-bone grafting at good toughness
Active (embodiment 1,2,3)；Be not added with nano-silver thread composite biological material intensity and poor toughness, without antibiotic property (comparative example 1)；
It is not added with a nanometer β-Ca3(PO4)2Composite biological material bone-inducting active it is poor (comparative example 2).
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (7)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810726129.9A CN109045368A (en) | 2018-07-04 | 2018-07-04 | A kind of medical high-strength degree high tenacity absorbable composite biomaterial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810726129.9A CN109045368A (en) | 2018-07-04 | 2018-07-04 | A kind of medical high-strength degree high tenacity absorbable composite biomaterial |
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