CN109045151A

CN109045151A - A kind of composition and preparation method thereof of regulation intestinal flora

Info

Publication number: CN109045151A
Application number: CN201811147197.6A
Authority: CN
Inventors: 缪应雷; 孙杨; 杨宇梅; 南琼; 马岚青; 王蓝; 顾雯茜; 牛俊坤; 张峰睿; 卜芝; 卜一芝
Original assignee: First Affiliated Hospital of Kunming Medical University
Current assignee: First Affiliated Hospital of Kunming Medical University
Priority date: 2018-09-29
Filing date: 2018-09-29
Publication date: 2018-12-21

Abstract

The present invention discloses a kind of composition and preparation method thereof of regulation intestinal flora, is related to intestinal flora control technique field.The composition of the regulation intestinal flora, the raw material including following parts by weight: 70-80 parts of buttered tea, 1-2 parts of apple polysaccharide, 3-5 parts of haw thorn extract, 4-6 parts of spirulina, 3-4 parts of propolis, papaya extractives 3-5,0.3-0.5 parts of probiotics powder agent；Using buttered tea as the composition of the regulation intestinal flora of major ingredient, intestinal flora can be regulated and controled；It can be widely applied to health care product, special used food and ordinary food, and advantage of lower cost, have universal usability, be suitble to promote the use of.

Description

A kind of composition and preparation method thereof of regulation intestinal flora

Technical field

The present invention relates to intestinal flora control technique field, be specifically related to it is a kind of regulate and control intestinal flora composition and its Preparation method.

Background technique

Modern microecology viewpoint thinks that the essence of life is the balance for the ecosystem that biology is formed with internal and external environment, The essence of disease is that the factors effect of various interference hosts transfinites and leads to ecological disturbance.It lives away from home in the gastrointestinal tract of Healthy People type Numerous microorganisms, these microorganisms are referred to as intestinal flora.Intestinal flora combines according to a certain percentage, makes mutually between each flora About, it depends on each other for existence, forms a kind of ecological balance on quality and quantity.Once body internal and external environment changes, as prolonged application is wide Antibiotic is composed, sensitive enterobacteriaceae is suppressed, and not repressed bacterium then seizes the opportunity breeding, normal raw so as to cause flora imbalance Reason combination is destroyed, and is generated pathologic combination and is caused clinical symptoms, referred to as intestinal bacilli illness disease.

Currently, the mode of adjustment intestinal flora is mainly with nutrition needed for probiotic supplemented type and quantity, probiotic supplemented Substance (the prebiotic factor) inhibits or kills harmful intestinal tract bacteria (mainly Gram-negative bacteria), enhances human immunity to improve Macrophage achievees the purpose that intestinal flora balance to modes such as the phagocytosis of harmful bacteria, discloses in the prior art a variety of Using the product of traditional probiotics and the prebiotic factor and combinations thereof for regulating and controlling intestinal flora, still, currently, existing regulation There is also the places of some shortcomings for the product of intestinal flora, and the product cost for such as regulating and controlling intestinal bacilli illness is relatively high, selected Medicinal material is also mostly the object of pharmacological property stimulation, there is certain injury to human body, and applicability is not wide, is not suitable for ordinary people and uses；At present still Lack the product with the regulation intestinal flora of general applicability.

Summary of the invention

In view of the above-mentioned problems existing in the prior art, the present invention provides the compositions and its system of a kind of regulation intestinal flora Preparation Method can regulate and control intestinal flora using buttered tea as the composition of the regulation intestinal flora of major ingredient；It can be widely applied to health care Product, special used food and ordinary food, and advantage of lower cost have universal usability, are suitble to promote the use of.

To realize above-mentioned technical purpose and the technique effect, the present invention is achieved through the following technical solutions:

A kind of composition of regulation intestinal flora, the raw material including following parts by weight: 70-80 parts of buttered tea, apple are more 1-2 parts sugared, 3-5 parts of haw thorn extract, 4-6 parts of spirulina, 3-4 parts of propolis, papaya extractives 3-5, probiotics powder agent 0.3- 0.5 part；

The probiotics powder agent is uniformly mixed by the pulvis of following parts by weight: 40-50 parts of lactobacillus plantarum, long double 30-45 parts of discrimination bacillus, 25-35 parts of bifidobacterium bifidum, 15-20 parts of bifidobacterium adolescentis, 15-20 parts of lactobacillus bulgaricus, 10-15 parts of lactobacillus acidophilus, 8-10 parts of Lactobacillus casei, 8-10 parts of streptococcus thermophilus, 8-10 parts of saccharomyces cerevisiae, Candida utilis ferment Female bacterium 4-6 parts.

Further, the composition of the control intestinal flora, the raw material including following parts by weight: 70 parts of buttered tea, apple 2 parts of fruit polysaccharide, 4 parts of haw thorn extract, 5 parts of spirulina, 3 parts of propolis, 4 parts of papaya extractives, 0.4 part of probiotics powder agent；

The probiotics powder agent is uniformly mixed by the pulvis of following parts by weight: 40 parts of lactobacillus plantarum, long bifid 35 parts of bacillus, 30 parts of bifidobacterium bifidum, 20 parts of bifidobacterium adolescentis, 15 parts of lactobacillus bulgaricus, lactobacillus acidophilus 15 Part, 9 parts of Lactobacillus casei, 9 parts of streptococcus thermophilus, 9 parts of saccharomyces cerevisiae, 5 parts of candida utili bacterium.

Adjuvant, which is added, in the composition of above-mentioned regulation intestinal flora can be prepared as pulvis, tablet, granule, capsule, liquid Body agent or drug, health food or beverage are edible for normal population.

Another object of the present invention is to provide a kind of preparation methods of the composition of regulation intestinal flora, including following step It is rapid:

1) prepared by raw material

Buttered tea is produced: first being heated milk, is subsequently poured into cream-milk separator, stirs so that water-oil separating, floats above The phat fat of one layer of lake yellow, cooling obtain butter；Later tealeaves or brick tea are first boiled into extract with water long, then tea is filtered After pour into buttered tea container, be put into the butter and salt prepared, stir to obtain oil tea blending, be then poured into pot and heat tanning, it is crisp Oil with tea juice is molten is integrated, be in emulsus；

Apple polysaccharide extracts: fresh apple be concentrated as cider, in the cider that will be concentrated with the dense poly- liquid of film filtering through alcohol Analysis, water-soluble impurity elimination, alcohol analyses to obtain apple polysaccharide again；

Haw thorn extract: dry haw raw material is removed into impurity and pulverization process, utilizes continuous flow upstream ultrasonic device pair Hawthorn fruit extracts；Extracting solution is concentrated, alcohol precipitation and is filtered；Extracting solution is isolated and purified by film device, Collect the higher haw thorn extract of purity；

Papaya extractives: splitting deseeding after pawpaw is cleaned, be cut into fragment, air-dries, is ground into powder, then passes through ethyl alcohol Middle immersion depressurizes rotary evaporation later and removes ethyl alcohol；Subsequent ultraviolet irradiation, then ethyl alcohol immersion is added, it is then centrifuged for collecting supernatant Supernatant is condensed into medicinal extract after dry and is ground into powder by liquid；

Probiotics powder agent: purchase object lactobacillus, bifidobacterium longum, bifidobacterium bifidum, bifidobacterium adolescentis, Bulgaria Lactobacillus, lactobacillus acidophilus, Lactobacillus casei, streptococcus thermophilus, saccharomyces cerevisiae, candida utili bacteria powder, later according to Special ratios are uniformly mixed,

Spirulina and propolis are directly bought；

2) apple polysaccharide extraction, haw thorn extract, papaya extractives spirulina and propolis are added to mixing in buttered tea, Mixture is heated after mixing, is stirred, is filtered after the completion of heating, is placed under room temperature cooling；

3) probiotics are added into mixture after cooling, are rocked, standing is regulated enterobacteriaceae after mixing evenly The composition of group, adjuvant, which can be added, can be prepared as pulvis, tablet, granule, capsule, liquid agent or drug, health food Or beverage is edible for normal population.

Further, the apple polysaccharide extraction includes filtering in dense poly- liquid to extract apple polysaccharide from film, takes film filtering dense Poly- liquid is heated to 60-100 DEG C, and the ethyl alcohol of 1-3 times of volume is added, obtains polysaccharide precipitate；1 times of polysaccharide volume is added in polysaccharide precipitate Boiling water dissolution, filtering, filtrate be concentrated into 1/2 volume, 2 times of volume ethanols of concentrate are added, obtain polysaccharide precipitate.

Further, the papaya extractives include splitting deseeding after cleaning pawpaw, are cut into fragment, air-dry, be ground into Powder is then placed in the ethyl alcohol that volume fraction is 70% and impregnates 30 minutes, is subject to and does not cross, and decompression rotary evaporation removes second Alcohol；Subsequent ultraviolet irradiation 15min, then add the ethyl alcohol that volume fraction is 85% and impregnate 30min, then 1000 turns/min centrifugation is received Collect supernatant, supernatant is condensed into the medicinal extract that density is 1.2g/ml and is ground into powder after 80 DEG C of dryings and obtains.

Further, described that apple polysaccharide extraction, haw thorn extract, papaya extractives spirulina and propolis are added to shortcake Mixing in oil tea carries out mixture after mixing to be heated to 60-70 °, be kept for temperature 20-30 minutes, and ceaselessly stirring is allowed to It is uniformly mixed, is filtered after the completion of heating, it is cooling to say that filtered mixture is placed under room temperature.

Further, the apple polysaccharide extract, haw thorn extract, papaya extractives, spirulina, propolis and buttered tea Probiotics are added at normal temperature and are uniformly mixed for mixture, probiotics can for above-mentioned probiotics or it is above-mentioned individually it is prebiotic Bacterium.

A kind of composition of regulation intestinal flora answering in preparation regulation intestinal flora drug, health food or beverage With.

It is a kind of regulate and control intestinal flora composition preparation improve individual immunity power drug in purposes.

Beneficial effects of the present invention: the present invention provides it is a kind of regulate and control intestinal flora composition and preparation method thereof, with Buttered tea is major ingredient, and is added to apple polysaccharide extract, haw thorn extract, papaya extractives, spirulina, propolis and probiotics On the one hand agent has preferable mouthfeel, while being remarkably improved body immunity and regulation intestinal flora, and then is increasing enteron aisle While probiotics flora type, when significantly enhancing colonization ability of the endogenous and external source probio in human body intestinal canal and field planting Between, the growth and breeding of harmful intestinal tract bacteria especially Gram-negative bacteria are effectively inhibited, beneficial bacteria of intestinal tract bacterium is sufficiently had adjusted The composition of group.It can be widely applied to preparation regulation intestinal flora drug, health food or application and preparation raising in beverage Application in the drug of individual immunity power, and advantage of lower cost have universal usability, are suitble to promote the use of.

Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.

Specific embodiment

Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described, Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention Embodiment, all other embodiment obtained by those of ordinary skill in the art without making creative efforts, all Belong to the scope of protection of the invention.

Embodiment 1

Probiotics powder agent mentioned by the present invention can pass through Online Shopping

A kind of composition of regulation intestinal flora, the raw material including following parts by weight: 80-90 parts of buttered tea, apple are more 1-2 parts sugared, 3-5 parts of haw thorn extract, 4-6 parts of spirulina, 3-4 parts of propolis, papaya extractives 3-5, probiotics powder agent 0.3- 0.5 part；

Further, the composition of the control intestinal flora, the raw material including following parts by weight: 70 parts of buttered tea, apple 2 parts of fruit polysaccharide, 4 parts of haw thorn extract, 5 parts of spirulina, 3 parts of propolis, papaya extractives 4,0.4 part of probiotics powder agent；

Embodiment 2

1) prepared by raw material

Apple polysaccharide extracts: fresh apple be concentrated as cider, including extracts apple polysaccharide from the dense poly- liquid of film filtering, is taken Film filters dense poly- liquid and is heated to 60-100 DEG C, and the ethyl alcohol of 1-3 times of volume is added, obtains polysaccharide precipitate；Polysaccharide precipitate is added 1 times The boiling water dissolution of polysaccharide volume, filtering, filtrate are concentrated into 1/2 volume, and 2 times of volume ethanols of concentrate are added, obtain polysaccharide precipitate For apple polysaccharide extract；

Papaya extractives: papaya extractives include splitting deseeding after cleaning pawpaw, are cut into fragment, air-dry, be crushed into powder End is then placed in the ethyl alcohol that volume fraction is 70% and impregnates 30 minutes, is subject to and does not cross, and decompression rotary evaporation removes ethyl alcohol； Subsequent ultraviolet irradiation 15min, then add the ethyl alcohol that volume fraction is 85% and impregnate 30min, then 1000 turns/min is collected by centrifugation Supernatant is condensed into the medicinal extract that density is 1.2g/ml and is ground into powder after 80 DEG C of dryings and obtains by supernatant.

Spirulina and propolis are directly bought；

2) apple polysaccharide extraction, haw thorn extract, papaya extractives spirulina and propolis are added to mixing in buttered tea, Mixture is carried out after mixing to be heated to 60-70 °, is kept for temperature 20-30 minutes, and ceaselessly stirring is allowed to uniformly mixed, adds It is filtered after the completion of heat, it is cooling to say that filtered mixture is placed under room temperature；

The apple polysaccharide extract, haw thorn extract, papaya extractives, spirulina, propolis and butter tea mixture exist Probiotics are added under room temperature and are uniformly mixed, probiotics can be above-mentioned probiotics or above-mentioned individual probiotics.

The composition of the regulation intestinal flora prepared in above-mentioned steps through organoleptic test, regulate and control the composition of intestinal flora at Product free from extraneous odour, smooth is sweet and refreshing, Hui Tian, has universal applicability.

Application effect of the invention is described in detail below with reference to experiment.

By the bacteria detection to SD rat excrement, using regulation intestinal flora of the invention composition and control group into Row comparative experiments:

Control experiment design:

20 SD rats are divided into four groups, every group 5；

First group is taken the composition for regulating and controlling intestinal flora of the invention daily；Second group is taken butter daily；Third group Take physiological saline, the 4th group take apple polysaccharide extract, haw thorn extract, papaya extractives, spirulina, propolis and prebiotic Microbial inoculum any one；

Respectively according to above-mentioned experimental design, equivalent timing is fed 15-20 days, takes the excrement of the SD rat of last day, into Row detection；

It is the results show that regulate and control the number of first group of macro base group of SD rat excrement of the composition of intestinal flora using the present invention It is found that a large amount of beneficial bacteriums are rich in, and in all control experiment groups, using the place of the composition of regulation intestinal flora of the invention Reason group beneficial bacterium quantity is much larger than other experimental groups, followed by uses the processing group of buttered tea, is thirdly using apple polysaccharide Any one processing group of extract, haw thorn extract, papaya extractives, spirulina, propolis and probiotics；

Further experiment, using the raw material of following parts by weight: 70 parts of buttered tea, 2 parts of apple polysaccharide, haw thorn extract 4 Part, 5 parts of spirulina, 3 parts of propolis, papaya extractives 4,0.4 part of probiotics powder agent；

The probiotics powder agent is uniformly mixed by the pulvis of following parts by weight: 40 parts of lactobacillus plantarum, long bifid 35 parts of bacillus, 30 parts of bifidobacterium bifidum, 20 parts of bifidobacterium adolescentis, 15 parts of lactobacillus bulgaricus, lactobacillus acidophilus 15 Part, 9 parts of Lactobacillus casei, 9 parts of streptococcus thermophilus, 9 parts of saccharomyces cerevisiae, 5 parts of candida utili bacterium and through the foregoing embodiment The composition of regulation intestinal flora of preparation step preparation 5 SD rats are fed, the amount of nursing and time with it is above-mentioned Test it is identical, feed 15-20 days, take the excrement of the SD rat of last day, detected；

Testing result shows that the beneficial bacterium that the data discovery of the macro base group of the excrement of the SD rat of further experiment is rich in omits Data higher than the above-mentioned macro base group of first group of SD rat excrement find the beneficial bacterium being rich in.

The present invention provides a kind of compositions and preparation method thereof of regulation intestinal flora, using buttered tea as major ingredient, and add Apple polysaccharide extract, haw thorn extract, papaya extractives, spirulina, propolis and probiotics are added, have on the one hand had preferable Mouthfeel, while being remarkably improved body immunity and regulation intestinal flora, and then increasing beneficial bacteria of intestinal tract flora type Meanwhile colonization ability and resident time of the endogenous and external source probio in human body intestinal canal are significantly enhanced, effectively inhibit intestines The growth and breeding of road harmful bacteria especially Gram-negative bacteria, sufficiently have adjusted the composition of beneficial bacteria of intestinal tract flora.It can be extensive Drug applied to application and preparation raising individual immunity power in preparation regulation intestinal flora drug, health food or beverage In application, and advantage of lower cost has universal usability, is suitble to promote the use of.

In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example. Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close Suitable mode combines.

Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification, It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only It is limited by claims and its full scope and equivalent.

Claims

1. A composition for regulating intestinal flora, characterized in that: it comprises the following raw materials in parts by weight: 70-80 parts of butter tea, 1-2 parts of apple polysaccharide, 3-5 parts of hawthorn extract, 4-5 parts of spirulina 6 parts, 3-4 parts of propolis, 3-5 parts of papaya extract, 0.3-0.5 parts of probiotic powder;

The probiotic powder is uniformly mixed by the following powders in parts by weight: 40-50 parts of Lactobacillus plantarum, 30-45 parts of Bifidobacterium longum, 25-35 parts of Bifidobacterium bifidum, 15 parts of Bifidobacterium adolescentis 20 parts, Lactobacillus bulgaricus 15-20 parts, Lactobacillus acidophilus 10-15 parts, Lactobacillus casei 8-10 parts, Streptococcus thermophilus 8-10 parts, Saccharomyces cerevisiae 8-10 parts, Candida utilis 4-6 servings.

2. The composition for controlling intestinal flora as claimed in claim 1, characterized in that: it comprises the following raw materials in parts by weight: 70 parts of butter tea, 2 parts of apple polysaccharide, 4 parts of hawthorn extract, 5 parts of spirulina, 3 parts of propolis, 4 parts of papaya extract, 0.4 part of probiotic powder;

The probiotic powder is uniformly mixed with the following powders by weight: 40 parts of Lactobacillus plantarum, 35 parts of Bifidobacterium longum, 30 parts of Bifidobacterium bifidus, 20 parts of Bifidobacterium adolescentis, 15 parts of Lactobacillus bulgaricus , 15 parts of Lactobacillus acidophilus, 9 parts of Lactobacillus casei, 9 parts of Streptococcus thermophilus, 9 parts of Saccharomyces cerevisiae, 5 parts of Candida utilis.

3. a preparation method of the composition of regulating intestinal flora as claimed in claim 1, is characterized in that, the preparation method of the composition of described regulating intestinal flora comprises the following steps:

1) Raw material preparation

Butter tea production: first heat the milk, then pour it into a cream separator, stir to separate the oil and water, and a layer of lake-yellow fat floats on it, and cool it to get butter; after that, boil the tea leaves or brick tea with water for a long time to make a thick juice. Then filter the tea water and pour it into the butter tea container, add the prepared butter and salt, stir until the oil tea blends, then pour it into the pot and heat it until the butter and tea juice are melted into one, and it becomes milky.

Extraction of apple polysaccharides: Concentrate fresh apples into apple juice, filter the concentrated apple juice with membrane to obtain apple polysaccharides through alcohol analysis, water dissolution to remove impurities, and alcohol analysis;

Hawthorn extract: The dried hawthorn raw material is cleaned of impurities and crushed, and the hawthorn powder is extracted by continuous countercurrent ultrasonic equipment; the extract is concentrated, alcohol-precipitated and filtered; the extract is separated and purified by membrane equipment, which can be collected Hawthorn extract with high purity;

Papaya extract: Wash the papaya, cut it open and remove the seeds, cut it into pieces, air-dry it, crush it into powder, then soak it in ethanol, and then remove the ethanol by rotary evaporation under reduced pressure; then irradiate with ultraviolet light, add ethanol to soak, and then centrifuge Collect the supernatant, concentrate the supernatant into an extract, dry it, and crush it into powder;

Probiotic powder: Lactobacillus, Bifidobacterium longum, Bifidobacterium bifidus, Bifidobacterium adolescent, Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Streptococcus thermophilus, Saccharomyces cerevisiae, Candida utilis Yeast powder, then mix evenly according to a specific ratio,

Spirulina and propolis purchased directly;

2) Add apple polysaccharide extract, hawthorn extract, papaya extract spirulina and propolis to butter tea and mix, heat and stir the mixture after mixing, filter after heating, and cool at room temperature;

3) Add probiotics to the cooled mixture, shake and stir evenly, and then stand still to obtain a composition for regulating intestinal flora, which can be prepared as powder, tablet, granule, capsule, liquid by adding auxiliary agents Drugs or medicines, health food or beverages for the normal population.

4. the preparation method of the composition of regulating intestinal flora as claimed in claim 3, is characterized in that: described apple polysaccharide extraction comprises extracting apple polysaccharide from membrane filtration concentrated liquid, gets membrane filtration concentrated liquid and is heated to 60-100 ℃, add 1-3 times the volume of ethanol, polysaccharide precipitates; polysaccharide precipitates are dissolved in boiling water with 1 times the volume of polysaccharides, filtered, the filtrate is concentrated to 1/2 volume, and 2 times the volume of ethanol is added to the concentrated solution to obtain Polysaccharide precipitates.

5. The preparation method of the composition regulating intestinal flora as claimed in claim 3, is characterized in that: described papaya extract comprises that after papaya is cleaned, cut open and remove seeds, cut into pieces, air-dried, pulverized into powder, then soaked in ethanol with a volume fraction of 70% for 30 minutes, subject to the ethanol, and removed the ethanol by rotary evaporation under reduced pressure; then irradiated with ultraviolet rays for 15 minutes, then added ethanol with a volume fraction of 85% for 30 minutes, and then 1000 rpm The supernatant was collected by centrifugation at 1/min, concentrated into an extract with a density of 1.2 g/ml, dried at 80° C., and crushed into powder.

6. The preparation method of the composition regulating intestinal flora as claimed in claim 3, characterized in that: the apple polysaccharide extract, hawthorn extract, papaya extract spirulina and propolis are added to butter tea and mixed, mixed Finally, heat the mixture to 60-70°, keep the temperature for 20-30 minutes, and keep stirring to make it evenly mixed, filter after heating, and place the filtered mixture at room temperature to cool.

7. The preparation method of the composition regulating intestinal flora as claimed in claim 3, is characterized in that: described apple polysaccharide extract, hawthorn extract, papaya extract, spirulina, propolis and butter tea mixture Add the probiotic agent and mix evenly, the probiotic agent can be the above-mentioned probiotic agent or the above-mentioned independent probiotic.

8. The composition for regulating intestinal flora according to any one of claims 1-2, characterized in that: adding auxiliary agents can be prepared as powder, tablet, granule, capsule, liquid or medicine, health care Food or drink is for normal population consumption.

9. Application of a composition for regulating intestinal flora according to any one of claims 1-7 in the preparation of medicines, health food or beverages for regulating intestinal flora.

10. The use of a composition for regulating intestinal flora according to any one of claims 1-7 in the preparation of medicines for improving individual immunity.