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CN109043063A - A kind of Yunnan arabiancoffee beans and its preparation process - Google Patents

A kind of Yunnan arabiancoffee beans and its preparation process Download PDF

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Publication number
CN109043063A
CN109043063A CN201810736542.3A CN201810736542A CN109043063A CN 109043063 A CN109043063 A CN 109043063A CN 201810736542 A CN201810736542 A CN 201810736542A CN 109043063 A CN109043063 A CN 109043063A
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China
Prior art keywords
honeybee
preparation process
process according
honey
days
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CN201810736542.3A
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Chinese (zh)
Inventor
杨波
侯佳欣
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Yunnan Benji Biotechnology Co Ltd
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Yunnan Benji Biotechnology Co Ltd
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Priority to CN201810736542.3A priority Critical patent/CN109043063A/en
Publication of CN109043063A publication Critical patent/CN109043063A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F5/00Coffee; Coffee substitutes; Preparations thereof
    • A23F5/02Treating green coffee; Preparations produced thereby
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F5/00Coffee; Coffee substitutes; Preparations thereof
    • A23F5/16Removing unwanted substances
    • A23F5/163Removing unwanted substances using enzymes or microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Jellies, Jams, And Syrups (AREA)

Abstract

The present invention relates to a kind of Yunnan arabiancoffee beans and its preparation processes, its technique from honey the following steps are included: isolate saccharomycete, carry out purification storage, coffee bean and white sugar after screening to be removed to pericarp are stored in container together while being added honeybee yeast and be sealed, stationary culture and periodic agitation is carried out under the conditions of 18-25 DEG C, it is filtered after 15-30 days, Yunnan arabiancoffee beans made of fermentation can be obtained.Compared to existing saccharomycete, the honeybee yeast in the present invention is resistant to -18 DEG C of freezings, easy to maintain, fragrance after manufactured coffee bean is baked, in good taste, and the sense of sweet tea acid balance is good.

Description

A kind of Yunnan arabiancoffee beans and its preparation process
Technical field
The present invention relates to technical field of food biotechnology, in particular to a kind of Yunnan arabiancoffee beans and its preparation process.
Background technique
Yeast is distributed in entire nature, is a kind of typical amphimicrobe, under aerobic and oxygen free condition all It can survive, be natural fermented dose a kind of.Saccharomycete is the quasi-microorganism for being applied earliest in history of human civilization, saccharomycete It is in close relations with the mankind, it is industrial most important, a most widely used quasi-microorganism, in brewing, food, medical industry etc. Aspect occupies an important position.Certain yeast can be grown in the few environment of moisture, such as honey and jam.
Yunnan arabiancoffee, Rubiaceae, Coffea, growing area are mainly distributed on Lincang, the Baoshan, Simao, Xishuangbanna, Dehong Etc. ground state.Maximum feature is that content of caffeine is low, and small to the irritation of stomach, mouthfeel is soft, and arabiancoffee suitable growth exists It is sour if height above sea level is too high on the mountainous region that 800~1800 meters of height above sea level, too low bitter.
Summary of the invention
It is an object of the invention to solve Yunnan arabiancoffee currently on the market shallowly to dry the dead acid of appearance often, can go out after finishing off The problems such as existing fermented soya bean taste, provides a kind of preparation method of Yunnan arabiancoffee, and the Yunnan arabiancoffee of technique preparation is than general The more yellow rice wine of coffee and caramel mixing it is fragrant, the tartaric acid etc. of similar red wine.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of preparation process of Yunnan arabiancoffee beans, preparation process the following steps are included:
(1) separation of honeybee barms is chosen: diluted honey is accessed in potato dextrose agar, Under the conditions of 28 ± 1 DEG C, cultivates 5-7 days, pick out strain and purified, obtain honeybee barms and save;
(2) the honeybee barms scribing line that step (1) obtains the culture of honeybee barms: is forwarded to potato grape In sugared agar medium, inoculum concentration is the 1-2% of potato dextrose agar weight, under the conditions of 28 ± 1 DEG C, culture 5-7 days, first class inoculum is made, then first class inoculum is inoculated into liquid culture medium;Inoculum concentration is the 1- of liquid culture medium weight 2%, stationary culture 3-7 days under conditions of 18-25 DEG C obtains honeybee yeast liquid strain;
(3) fresh Yunnan arabiancoffee is chosen, coffee drupe is screened using immersion way, then the fruit after screening is removed Pericarp obtains green coffee.
(4) green coffee in step (3) is packed into jar fermenter, the white sugar and honey of green coffee weight 18%-24% is added Bee yeast liquid strain covers cylinder mouth with gauze, and stationary culture is simultaneously periodically stirred, was carried out after 15-30 days by gauze Filter, can be obtained the green coffee fermented;
(5) green coffee fermented in step (4) is dried after washing, and Yunnan arabiancoffee beans both can be obtained.
Preferably, diluted honey is the diluted honey that honey is diluted by 10 multiple in step (1).
Preferably, the diluted honey for choosing 3 dilutions of 1:10,1:100,1:1000 in step (1) connects respectively Enter in potato dextrose agar.
Preferably, the separation of honeybee yeast is derived from the village the Guan Chuan honeybee base of Thousand-Island Lake Maple Ridge town in step (1) Honey.
Preferably, the potato dextrose agar formula in step (1)-(2) are as follows: potato 20%, grape Sugar 2.0%, agar 2.0%, water surplus.
Preferably, liquid culture medium formula in step (2) are as follows: fruit 50%, white sugar 20-30%, water surplus.
Preferably, fruit preferably apple and/or Kiwi berry.
Preferably, periodic agitation is that every 1 day, thoroughly stirring was primary after 3 days in step (4), such honeybee barms It can preferably be fermented using the white sugar of addition, and convert polysaccharide for fructose, white sugar.
Preferably, washing is 3-5 times in step (5), the sugar on raw beans surface is washed away, is conducive to later period drying, drying control Moisture processed is in 12-15%.
Compared with prior art, the invention has the following advantages that
1. the honeybee barms tolerance separated in the present invention is good, can survive in -18 DEG C.
2. product of the invention so that mouthfeel, fragrance are more abundant, and is converted fructose and white sugar to more sharp by fermentation In the polysaccharide of human body.
3. operation of the present invention simple possible is suitble to industrialized production.
Specific embodiment
Next combined with specific embodiments below invention is further explained, but does not limit the invention to these tools Body embodiment.One skilled in the art would recognize that present invention encompasses may include in Claims scope All alternatives, improvement project and equivalent scheme.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art. Method in following embodiments is unless otherwise instructed the conventional method of this field.
Embodiment 1
It separates honeybee barms: the honey in the village the Guan Chuan honeybee base of Thousand-Island Lake Maple Ridge town is pressed to 10 times Number dilution, 3 dilutions for choosing 1:10,1:100,1:1000 are respectively connected in potato dextrose agar, are inoculated with Amount is 1 milliliter, under the conditions of 28 DEG C, cultivates 5-7 days, barms is picked out, in potato dextrose agar plate Upper scribing line culture is purified, and pure honeybee barms can be obtained after cultivating by 3-5 scribing line.
Preparation level-one honeybee barms: honeybee barms are forwarded in potato dextrose agar, are connect Kind amount is the 1% of potato dextrose agar, under the conditions of 28 DEG C, cultivates 5 days, first class inoculum is made.
Potato dextrose agar formula are as follows: potato cleans peeling, weighs 200g and is cut into small pieces, adds boiling rotten (boiling 20~30 minutes, can be poked by glass bar), with eight layers of filtered through gauze, heating adds 20g agar, continues heating and stirs Mixing is mixed, after agar has dissolved, glucose is added, stirs evenly, supplies moisture again after slightly cooling down to 1000 milliliters, packing tries Pipe, jumps a queue, wraps up, and (121 DEG C) the rear taking-up test tubes of sterilizing 20 minutes or so put inclined-plane.
Embodiment 2
1) prepare honeybee yeast liquid strain: level-one honeybee barms obtained are inoculated into liquid culture in embodiment 1 In base, inoculum concentration is the 1% of liquid culture medium weight, cultivates 7 days under the conditions of 18 DEG C, obtains honeybee yeast liquid strain.
Liquid culture medium formula are as follows: apple 50%, white sugar 20%, water surplus, natural PH.
2) it weighs and handles rear green coffee 10kg well and 1.8kg white sugar is fitted into cylinder, honeybee yeast liquid bacterium is added in midway Kinds 5 milliliters, cylinder mouth is tightened with gauze, and 18 DEG C or so stationary cultures, since the 3rd day ing after 1 day stirs 1 time, 25 days The green coffee fermented can be obtained in filtering.
Embodiment 3
1) prepare honeybee yeast liquid strain: level-one honeybee barms obtained are inoculated into liquid culture in embodiment 1 In base, inoculum concentration is the 2% of liquid culture medium weight, cultivates 7 days under the conditions of 18 DEG C, obtains honeybee yeast liquid strain.
Liquid culture medium formula are as follows: apple 50%, white sugar 30%, water surplus, natural PH.
2) it weighs and handles rear green coffee 10kg well and 2.0kg white sugar is fitted into cylinder, honeybee yeast liquid bacterium is added in midway Kinds 7 milliliters, cylinder mouth is tightened with gauze, and 18 DEG C or so stationary cultures, since the 3rd day ing after 1 day stirs 1 time, 20 days The green coffee fermented can be obtained in filtering.
Embodiment 4
1) prepare honeybee yeast liquid strain: level-one honeybee barms obtained are inoculated into liquid culture in embodiment 1 In base, inoculum concentration is the 1% of liquid culture medium weight, cultivates 7 days at 20 °C, obtains honeybee yeast liquid strain.
Liquid culture medium formula are as follows: Kiwi berry 50%, white sugar 20%, water surplus, natural PH.
2) it weighs and handles rear green coffee 10kg well and 2.2kg white sugar is fitted into cylinder, honeybee yeast liquid bacterium is added in midway Kinds 8 milliliters, cylinder mouth is tightened with gauze, and 18 DEG C or so stationary cultures, since the 3rd day ing after 1 day stirs 1 time, 18 days The green coffee fermented can be obtained in filtering.
Embodiment 5
1) prepare honeybee yeast liquid strain: level-one honeybee barms obtained are inoculated into liquid culture in embodiment 1 In base, inoculum concentration is the 2% of liquid culture medium weight, cultivates 7 days at 20 °C, obtains honeybee yeast liquid strain.
Liquid culture medium formula are as follows: Kiwi berry 50%, white sugar 30%, water surplus, natural PH.
2) it weighs and handles rear green coffee 10kg well and 2.4kg white sugar is fitted into cylinder, honeybee yeast liquid bacterium is added in midway Kinds 8 milliliters, cylinder mouth is tightened with gauze, and 18 DEG C or so stationary cultures, since the 3rd day ing after 1 day stirs 1 time, 15 days The green coffee fermented can be obtained in filtering.
After -18 DEG C of honeybee barms be prepared by embodiment 1 save 1 month detection saccharomycete quantity 1 × 107A/gram.
There is stronger vinasse taste by the green coffee that embodiment 2-5 is prepared, the beans of similar red wine processing.Shallowly dried Dry perfume (or spice) has the fragrance of stronger caramel and yellow rice wine mixing after training milling, and the wet fragrant initial stage after brewing is fragrant in apparent almond, the later period Switch to yellow rice wine perfume, the sense of sweet tea acid balance is good, tart flavour similar to red wine tartaric acid, it is in good taste.Finishing off bottom of a cup does not have general Yunnan coffee bean The fermented soya bean taste that will appear, but the mixing of yellow rice wine and caramel is fragrant.
The above, the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although referring to before Stating embodiment, invention is explained in detail, those skilled in the art should understand that: it still can be to preceding Technical solution documented by each embodiment is stated to modify or equivalent replacement of some of the technical features;And these It modifies or replaces, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.

Claims (10)

1. a kind of preparation process of Yunnan arabiancoffee beans, it is characterised in that the production technology the following steps are included:
(1) separation of honeybee barms is chosen: diluted honey is accessed in potato dextrose agar, 28 ± Under the conditions of 1 DEG C, cultivates 5-7 days, pick out strain and purified, obtain honeybee barms and save;
(2) the honeybee barms scribing line that step (1) obtains the culture of honeybee barms: is forwarded to potato glucose fine jade In rouge culture medium, inoculum concentration is the 1-2% of potato dextrose agar weight, under the conditions of 28 ± 1 DEG C, cultivates 5-7 First class inoculum is made in it, and then first class inoculum is inoculated into liquid culture medium;Inoculum concentration is the 1-2% of liquid culture medium weight, Stationary culture 3-7 days under conditions of 18-25 DEG C obtains honeybee yeast liquid strain;
(3) fresh Yunnan arabiancoffee is chosen, coffee drupe is screened using immersion way, then the fruit after screening is removed into pericarp Obtain green coffee.
(4) green coffee in step (3) is packed into jar fermenter, the white sugar and honeybee ferment of green coffee weight 18%-24% is added Female liquid strain covers cylinder mouth with gauze, and stationary culture is simultaneously periodically stirred, is filtered after 15-30 days by gauze, i.e., The green coffee that can be fermented;
(5) green coffee fermented in step (4) is dried after washing, and Yunnan arabiancoffee beans both can be obtained.
2. a kind of preparation process according to claim 1, which is characterized in that diluted honey is pressed in step (1) for honey The diluted honey that 10 multiple is diluted.
3. a kind of preparation process according to claim 1, which is characterized in that choose 1:10,1:100,1 in step (1): The diluted honey of 1000 3 dilutions is respectively connected in potato dextrose agar.
4. a kind of preparation process according to claim 1, which is characterized in that the honeybee is derived from Thousand-Island Lake Maple Ridge town official Honey in river village honeybee base.
5. a kind of preparation process according to claim 1, which is characterized in that the potato dextrose agar is matched Side are as follows: potato 20%, glucose 2.0%, agar 2.0%, water surplus.
6. a kind of preparation process according to claim 1, which is characterized in that the liquid culture medium formula are as follows: fruit 50%, white sugar 20%-30%, water surplus.
7. a kind of preparation process according to claim 1, which is characterized in that the fruit is selected from apple and/or Kiwi berry.
8. a kind of preparation process according to claim 1, which is characterized in that periodic agitation is thoroughly to stir after 3 days every 1 day It mixes primary.
9. a kind of preparation process according to claim 1, which is characterized in that washing is 3-5 times in step (5), washes away life The sugar on beans surface, drying control moisture is in 12-15%.
10. a kind of Yunnan arabiancoffee beans that preparation process described in one of -9 is prepared according to claim 1.
CN201810736542.3A 2018-07-06 2018-07-06 A kind of Yunnan arabiancoffee beans and its preparation process Pending CN109043063A (en)

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CN201810736542.3A CN109043063A (en) 2018-07-06 2018-07-06 A kind of Yunnan arabiancoffee beans and its preparation process

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Application Number Priority Date Filing Date Title
CN201810736542.3A CN109043063A (en) 2018-07-06 2018-07-06 A kind of Yunnan arabiancoffee beans and its preparation process

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109938138A (en) * 2019-03-27 2019-06-28 云南省农业科学院热带亚热带经济作物研究所 A kind of coffee fresh fruit processing method improving coffee bean flavor

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CN1870899A (en) * 2003-09-25 2006-11-29 三得利株式会社 Method of processing green coffee beans
CN101040653A (en) * 2005-03-24 2007-09-26 三得利株式会社 Method of processing fresh coffee beans by using surface-treated coffee fruits
CN103349137A (en) * 2013-07-09 2013-10-16 德宏后谷咖啡有限公司 Production method for winey coffee beans
CN104719576A (en) * 2015-04-09 2015-06-24 德宏后谷咖啡有限公司 Yunnan arabica coffee bean preparation technology
CN105192212A (en) * 2014-06-11 2015-12-30 英发国际股份有限公司 Biomimetic fermentation method of green coffee beans

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Publication number Priority date Publication date Assignee Title
CN1870899A (en) * 2003-09-25 2006-11-29 三得利株式会社 Method of processing green coffee beans
CN101040653A (en) * 2005-03-24 2007-09-26 三得利株式会社 Method of processing fresh coffee beans by using surface-treated coffee fruits
CN103349137A (en) * 2013-07-09 2013-10-16 德宏后谷咖啡有限公司 Production method for winey coffee beans
CN105192212A (en) * 2014-06-11 2015-12-30 英发国际股份有限公司 Biomimetic fermentation method of green coffee beans
CN104719576A (en) * 2015-04-09 2015-06-24 德宏后谷咖啡有限公司 Yunnan arabica coffee bean preparation technology

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109938138A (en) * 2019-03-27 2019-06-28 云南省农业科学院热带亚热带经济作物研究所 A kind of coffee fresh fruit processing method improving coffee bean flavor

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